Consulta: subjectFacets:"Experimental infection"
Registros recuperados: 31
Data/hora: 03/06/2024 16:25:58

Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples
Provedor de dados: 5
Autores: Schikorski, David; Faury, Nicole; Pepin, Jean-francois; Saulnier, Denis; Tourbiez, Delphine; Renault, Tristan.
Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas.  In the present work, we have developed an experimental protocol of horizontal transmission based on the cohabitation between healthy and experimentally infected oysters. Through a cohabitation trial, the kinetics of OsHV-1 detection in different oyster organs and seawater samples were investigated and characterized for the first time using real time quantitative PCR.
Tipo: Text
Palavras-chave: Ostreid herpesvirus 1; Cupped Pacific oyster; Crassostrea gigas; Experimental infection; Kinetic viral DNA quantification.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00025/13607/10688.pdf

Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf

Fasciola hepatica-Pseudosuccinea columella interaction: effect of increasing parasite doses, successive exposures and geographical origin on the infection outcome of susceptible and naturally-resistant snails from Cuba
Provedor de dados: 5
Autores: Alba, Annia; Vazquez, Antonio A.; Sanchez, Jorge; Duval, David; Hernandez, Hilda M.; Sabourin, Emeline; Vittecoq, Marion; Hurtrez-bousses, Sylvie; Gourbal, Benjamin.
Background: Pseudosuccinea columella is one of the most widespread vectors of Fasciola hepatica, a globally distributed trematode that affects humans, livestock and wildlife. The exclusive occurrence in Cuba of susceptible and naturally-resistant populations to F. hepatica within this snail species, offers a fascinating model for evolutionary biology, health sciences and vector control strategies. In particular, resistance in P. columella is characterized by the encapsulation of the parasite by host's immune cells and has been experimentally tested using different Cuban F. hepatica isolates with no records of successful infection. Here, we aimed to explore for the first time, the effect of different parasite doses, successive exposures and different...
Tipo: Text
Palavras-chave: Snail-trematode interaction; Lymnaeidae; Liver fluke; Experimental infection; Immune priming; Compatibility; Allopatric parasites.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00467/57894/71760.pdf

Experimental herpes-like viral infections in marine bivalves: demonstration of interspecies transmission
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan; Lipart, Cecile.
Since 1972, herpes-like virus infections have been reported in several marine bivalve species around the world. Viral detection was often associated with high mortality rates in larvae and spat. To determine whether a single virus is able to infect different bivalve host species, we carried out experimental transmission assays. As a first step, 8 assays were performed to infect axenic Crassostrea gigas larvae with virus from infected C. gigas larvae using a previously described protocol. The protocol appeared reliable and PCR was confirmed as a powerful technique for detecting viral DNA in experimentally infected oysters. The defined protocol was then applied to infect different bivalve species. Interspecies viral transmission was demonstrated under...
Tipo: Text
Palavras-chave: Experimental infection; Host specificty; Interspecies transmission; Marine bivalves; Herpes like virus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-819.pdf

Experimental infection of adult Scapharca broughtonii with Ostreid herpesvirus SB strain
Provedor de dados: 5
Autores: Bai, Chang-ming; Wang, Qing-chen; Morga, Benjamin; Shi, Jie; Wang, Chong-ming.
We investigated the susceptibility of ark shell, Scapharca broughtonii, adults to Ostreid herpesvirus SB strain (OsHV-1-SB) through experimental infection by intramuscular injection assays. Results showed the onset of mortality occurred at 3 days post injection, one day after the water turbidity became evident in rearing tanks. The mortality curves for the challenged group were similar to those observed at affected hatcheries. Histological lesions, herpesvirus-like particles and high OsHV-1-SB quantities were detected in challenged ark shells. This is the first study to successfully reproduce OsHV-1 disease in Arcoida species, and very few studies in adult bivalves (over 24 months old).
Tipo: Text
Palavras-chave: Scapharca broughtonii; Ostreid herpesvirus SB strain; OsHV-1-SB; Experimental infection; Viral quantity.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00360/47119/47081.pdf

Vibrio tubiashii en France: description d'isolats pathogènes affectant des mollusques et étude de leurs mécanismes de virulence
Provedor de dados: 5
Autores: Mersni Achour, Rachida.
The oyster farming constitutes one of the major components of the global aquaculture. However, this sector is facing abnormal mortalities outbreaks that affect diploid and triploid oyster at their different life stages, in the hatcheries and in the field. During bivalve molluscs mortality events in France, bacteria initially classified into Harveyi group, were regularly isolated along with herpes virus, V. splendidus or V. aestuarianus. In order to fine tune the taxonomic affiliation of those isolates, a genotypic and phenotypic approach was used. The bacterial isolates, initially misclassified into the Harveyi clade, were shown to be genetically closed to V. tubiashii strains already recognized as the main causative agents of larvae and juvenile mollusc...
Tipo: Text
Palavras-chave: Crassostrea gigas; Isolats français; Vibrio tubiashii; Hémocytes; Pathogénicité; Infection expérimentale; Produits extracellulaires; Facteurs de virulence; Metalloprotéases; Crassostrea gigas; French isolates; Vibrio tubiashii; Hemocytes; Pathogenicity; Experimental infection; Extracellular products.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00230/34093/32540.pdf

Crassostrea gigas mortality in France: the usual suspect, a herpes virus, may not be the killer in this polymicrobial opportunistic disease
Provedor de dados: 5
Autores: Petton, Bruno; Bruto, Maxime; James, Adele; Labreuche, Yannick; Alunno-bruscia, Marianne; Le Roux, Frederique.
Successive disease outbreaks in oyster (Crassostrea gigas) beds in France have resulted in dramatic losses in production, and subsequent decline in the oyster-farming industry. Deaths of juvenile oysters have been associated with the presence of a herpes virus (OsHV-1 µvar) and bacterial populations of the genus Vibrio. Although the pathogenicity of OsHV-1 µvar, as well as several strains of Vibrio has been demonstrated by experimental infections, our understanding of the complexity of infections occurring in the natural environment remains limited. In the present study, we use specific-pathogen-free (SPF) oysters infected in an estuarine environment to study the diversity and dynamics of cultured microbial populations during disease expression. We observe...
Tipo: Text
Palavras-chave: Pacific oysters; Summer mortality; Herpes virus; Vibrio pathogenicity; Experimental infection.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00271/38220/36377.pdf

Siderophore production and probiotic effect of Vibrio sp. associated with turbot larvae, Scophthalmus maximus
Provedor de dados: 5
Autores: Gatesoupe, Fj.
The proportion of vibrio E, dominant in healthy turbot larvae, was artificially increased in the rotifer enrichment medium. The maid effect of this vibrio enrichment was to improve the resistance of larval turbot challenged with a pathogenic strain of Vibrio splendidus, vibrio P. The improvement of the survival rates was significant with 1.5 x 10(6) CFU of vibrio E added per mi of enrichment medium, and it was still maximum at the hi hest dose tested (5 x 10(7) CFU.ml(-1); 80 +/- 2% of survivors after 48 h of challenge, versus 50 +/- 2 % for the control group). Further in vitro characterisation showed that vibrio E was able to grow in the presence of the chelating agents EDDHA and EDTA, while producing siderophore, as revealed by chrome azurol S dyeing....
Tipo: Text
Palavras-chave: Bacteria; Pathogenic Vibrio; Probiotic Vibrio; Experimental infection; Siderophore; Turbot; Larva.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00065/17671/15199.pdf

Experimental infection with Leishmania chagasi in immunosuppressed Balb/c mice: cytokines and parasite burdens
Provedor de dados: 80
Autores: Hoffmann,JL; Machado,JG; Gaio,FC; Dias-Melicio,LA; Langoni,H.
The immune response in leishmaniasis may result in a polarization of the T lymphocyte subpopulation, altering cell phenotype and resulting in immune protection or disease exacerbation. Leishmania may persist in the body either during asymptomatic infections or after treatment, which represents high risk under immunosuppression. The objective of this study was to evaluate the effect of infection with immunosuppression by dexamethasone associated with pentoxifylline on animal weight, spleen weight, spleen and hepatic parasitic load and immunopathology, as well as the IFN-γ and IL-10 production in spleen cell culture of Balb/c mice infected with Leishmania chagasi. The infection did not cause body weight gain in animals, but both the weight and size of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania chagasI; Immunosuppression; Cell-mediated response; Parasite burden; Experimental infection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300004

Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected
Provedor de dados: 80
Autores: Langoni,H.; Das Dores,C. B.; Silva,R. C.; Pezerico,S. B.; Castro,A. P. B.; Da Silva,A. V.
Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agent's DNA was not detected in any sample of the other experimental groups. New studies must be carried out to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmosis; PCR; Serum; Experimental infection; Mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200004

The Pathogenic Effects of a UK field Isolate of Eimeria tenella in Modern Broiler and Layer Chickens
Provedor de dados: 19
Autores: Marshall, R. N.; Taylor, M. A.; Green, J. A.; Catchpole, J.
Palavras-chave: Coccidiosis; Histopathological fidings; Bird; Experimental infection.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/227

Susceptibility of different mouse strains to experimental infection with a Venezuelan isolate of Trypanosoma evansi
Provedor de dados: 19
Autores: Perrone Carmona, Trina Mercedes; Garrizzo, Juan; Roschman-González, Antonio; Tejero, Felix; Escalante, Ananías; Aso, Pedro María.
The susceptibility of BALB/c, C57Bl/6, DBA/2, NMRI, NIH and CD1 mouse strains to experimental infection with a Venezuelan equine isolate of Trypanosoma evansi was examined through hemoglobin concentration, packed red cell volume, number of erythrocytes per blood μl, parasitemia, body weight, and mice survival time. The said variables seem to be suitable markers to contrast the mouse susceptibility to T. evansi experimental infections. Among the evaluated mouse strains, NMRI was the more susceptible and DBA/2 the less vulnerable to the local T. evansi isolate used in the study. The resistant mouse strains could help in the identification of factors conferring natural resistance against T. evansi, while the susceptible could be used to investigate the...
Palavras-chave: Trypanosoma evansi; Mouse susceptibility; Experimental infection.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/141

Kinetic analysis of specific antibodies in serum and urine of experimentally Encephalitozoon cuniculi-infected rabbits
Provedor de dados: 19
Autores: Oda, M.; Ueno, A.; Adilbish, A.; Li, Z.; Hernandez. H., O.; Okada, T.; Furuya, K.; Igarashi, M.
Encephalitozoonosis is a common infectious disease caused by Encephalitozoon cuniculi in rabbits. The purpose of this study was to examine the levels of antibody in urines of experimentally E. cuniculi-infected rabbits. Humoral immune response and spore excretion in urines were also examined. Rabbits were orally infected with E. cuniculi, and sera and urines were collected. Levels of serum IgG, IgM, IgA and urinary IgG were examined. The presences of spores in urines were examined by quantitative PCR. Specific IgG in sera started to be detected at day 20 after infection and kept high level during the course of experiment. Serum IgM started to be detected 1 week earlier than that of serum IgG and decreased to almost undetectable level by day 100 after...
Palavras-chave: Encephalitozoonsosis; Experimental infection; Urinary antibody.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4494

Experimental infection of dogs with Babesia microti
Provedor de dados: 19
Autores: Ohmori, T.; Uetsuka, K.; Nunoya, T.
Palavras-chave: Babesia microti; Dog; Experimental infection; Polymerase chain reaction; Indirect immunofluorescence test.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3132

北海道における夏季乳房炎 : Actinomyces pyogenesによる実験的乳房炎の臨床と病理学的所見
Provedor de dados: 19
Autores: 米道, 裕彌; 更科, 孝夫; Yonemichi, Hiromi; Sarashina, Takao.
Palavras-chave: 夏季乳房炎; 実験感染; Actinomyces pyogenes; Smmer mastitis; Experimental infection.
Ano: 1991
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1640

Metodologia de infecção experimental e grau de susceptibilidade do híbrido "tambacu" e Leporinus macrocephalus Garavello &amp;amp; Britski (Osteichthyes, Anostomidae) a quatro inóculos de trofozoítos de Icthyophthirius multifiliis Fouquet (Protozoa, Ciliophora)
Provedor de dados: 104
Autores: Souza,Valéria Nogueira de; Martins,Maurício Laterça; Moraes,Flávio Ruas de; Kronka,Sérgio do Nascimento.
Tropical fishes like Leporinus macrocephalus Garavello &amp;amp; Britski, 1988 ("piauçu") and the hybrid "tambacu" (Piaractus mesopotamicus Holmberg, 1887 "pacu" male x Colossoma macropomum Cuvier, 1818 "tambaqui" female) were experimentally infected with four inocula of trophonts of Ichthyophthirius multifiliis Fouquet, 1876 (Protozoa, Ciliophora) and the infection level was evaluated. Each one of twelve experimental units consisted of a tank with 200 liters of water and two specimens of each kind of fish. The following treatments were designed to each tank, in triplicate: 8, 16,32 and 64 trophonts. After 8 days of exposure the fishes were killed and the number of trophonts was counted at a stereoscopic microscope. This assay showed to be feasible at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ichthyophthirius multifiliis; Tropical fish; Experimental infection; Susceptibility.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000300016

Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil - doi: 10.4025/actascibiolsci.v32i3.5471
Provedor de dados: 3
Autores: Buglione, Celso Carlos; UFSC; Vieira, Felipe do Nascimento; UFSC; Mouriño, José Luiz Pedreira; UFSC; Pedrotti, Fabiola Santiago; UFSC; Jatoba, Adolfo; UFSC; Martins, Maurício Laterça; UFSC.
This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their  virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7%...
Tipo: Resistencia a infecção
Palavras-chave: 2.12.01.02-1 Shrimp; Experimental infection; Vibrio; Virulence; Immunology bacteriologia Shrimp; Experimental infection; Vibrio; Virulence; Immunology.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5471

Parameters for evaluation of clinical trial in mice infected with Trypanosoma cruzi
Provedor de dados: 45
Autores: Falkowski,G.J.S.; Sandri,P.F.; Tiyo,R.; Aleixo,D.L.; Araújo,S.M.
The effectiveness of clinical parameters in the evaluation of Trypanosoma cruzi infection was analyzed in male Swiss mice at 8 weeks old Animals were divided into HG (healthy) and IG (1400 trypomastigotes, intraperitoneally, Y strain - Trypanosoma cruzi). Quantitative and qualitative parameters were evaluated in non-consecutive days in the period, from 7th-11th and 15th-18th days of infection. There were significant differences (P&lt;0.05) between both groups in both periods regarding water consumption, abdominal circumference and weight. The second group presented differences in amount of excreta, body temperature, move-up and mortality. There was no difference (P&amp;gt;0.05) between the groups in food consumption, exploration of self-cleaning and skin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clinical analysis; Chagas disease; Experimental infection; Mice; Trypanosoma cruzi.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600020

Bovine respiratory syncytial virus: immunohistochemichal detection in mouse and bovine tissues using a Mab against human respiratory syncytial virus
Provedor de dados: 45
Autores: Almeida,R.S.; Spilki,F.R.; Roehe,P.M.; Verinaud,L.M.C.; Arns,C.W.
An immunoistochemical (IHC) test was developed to detect bovine respiratory syncytial virus (BRSV) in cell cultures and tissues of experimentally infected mice and calves, using a commercial monoclonal antibody (Mab) against human respiratory syncytial virus (HRSV), as a less expensive alternative, instead of producing specific monoclonal antibodies to BRSV. Clinical samples from calves suffering respiratory disease were also submitted to this test. IHC detected BRSV antigens in mouse tracheas (3, 5 and 7 days post-infection) and lungs (5 and 7 days post-infection), and in one of three lungs from experimentally infected calves. Lungs samples from two naturally infected calves were tested and resulted positive for BRSV by the IHC test. These results suggest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine respiratory syncytial virus; Immunohistochemistry; Calf; Mice; Experimental infection; Monoclonal antibody.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600001

Experimental Trypanosoma evansi infection in donkeys: hematological, biochemical and histopathological changes
Provedor de dados: 45
Autores: Cadioli,F.A.; Marques,L.C.; Machado,R.Z.; Alessi,A.C.; Aquino,L.P.C.T.; Barnabé,P A.
Five adult donkeys were experimentally infected with Brazilian strain of Trypanosoma evansi originally isolated from a naturally infected dog to study the hematological biochemical and histopathological alterations during the evolution of the disease. The course of the experimental infection was followed up to 145 days. Hematological analyses of the infected donkeys revealed a marked decline in hemoglobin, packed-cell volume, and erythrocyte count. Anemia was observed after successive peaks of parasitemia. Biochemical analyses showed increased levels of icterus index, serum globulins and decreased serum albumin and glucose values. All infected donkeys revealed enlargement of spleen and its white pulp, enlargement of mediastinal lymph nodes and lungs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Donkey; Experimental infection; Trypanosoma evansi; Trypanosomiasis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500008

Experimental infection in Notodiaptomus sp. (Crustacea: Calanoida) with larvae of Camallanus sp. (Nematoda: Camallanidae)
Provedor de dados: 45
Autores: Martins,M.L.; Ghiraldelli,L.; Garcia,F.; Onaka,E.M.; Fujimoto,R.Y.
This trial registered the experimental infection viability with nematode larvae Camallanus sp. in Notodiaptomus sp., a crustacean, which can be an intermediate host. Adult females of nematode were dissected from the intestines of Xiphophorus maculatus (Osteichthyes: Poeciliidae), at a fish farm in the State of São Paulo. Females were slightly compressed for larvae release, collected with Pasteur pipette and separated on Petri dishes with 9ml filtered water at 28.1ºC, from zooplankton culture. Treatments consisted of Petri dishes with 60 and 105 copepods, in which 120, 150 and 210 larvae of nematode were added in four replications. Twenty-four and 36h after exposition to the larvae, the copepods were fixed in 70% alcohol to record the amount of fixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental infection; Nematoda; Camallanus; Crustacea; Notodiaptomus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200018

Effects of experimental infections with larvae of Eustrongylides ignotus Jäegerskiold, 1909 and Contracaecum multipapillatum (Drasche, 1882) Baylis, 1920 in rabbits
Provedor de dados: 45
Autores: Barros,L.A.; Tortelly,R.; Pinto,R.M.; Gomes,D.C.
Rabbits were infected per os with 10 Eustrongylides ignotus L4 and with 50 Contracaecum multipapillatum L3 per rabbit, recovered from naturally infected freshwater fishes (Hoplias malabaricus) in order to evaluate the patogenicity of these two nematode species in mammalian host. Two rabbits (20%) infected with E. ignotus died before the fourth day post-inoculation (one after 51 and the other after 78 hours). Six rabbits (60%) were inappetent until the fifth day following experimental inoculation. No clinical signs in rabbits inoculated with C. multipapillatum were observed; nevertheless, eight (80%) animals were positive for this nematode species. Rabbits inoculated with E. ignotus, had gastric congestion with hematoma of the gastric wall in 60% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eustrongilydes ignotus; Contracaecum multipapillatum; Experimental infection; Rabbits; Pathology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300007

Association between polyclonal B cell activation and the presence of autoantibodies in mice infected with Yersinia enterocolitica O:3
Provedor de dados: 56
Eight-week old conventional female Swiss mice were inoculated intravenously with Yersinia enterocolitica O:3. A second group of normal mice was used as control. Five mice from each group were bled by heart puncture and their spleens were removed for spleen cell collection on the 3rd, 5th, 7th, 10th, 14th and 21st day after infection. Immunoglobulin-secreting spleen cells were detected by the isotype-specific protein A plaque assay. Total immunoglobulin levels were determined in mouse serum by single radial immunodiffusion and the presence of autoantibodies was determined by ELISA. We observed a marked increase in the total number of cells secreting immunoglobulins of all isotypes as early as on the 3rd day post-infection and the peak of secretion occurred...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Yersinia enterocolitica; Polyclonal activation; Experimental infection; Autoantibodies.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300015

Experimental murine mycobacteriosis: evaluation of the functional activity of alveolar macrophages in thalidomide- treated mice
Provedor de dados: 56
Autores: Arruda,M.S.P.; Richini,V.B.; Oliveira,S.M.A.; Vilani-Moreno,F.R.
Thalidomide is a selective inhibitor of tumor necrosis factor-alpha (TNF-alpha), a cytokine involved in mycobacterial death mechanisms. We investigated the role of this drug in the functional activity of alveolar macrophages in the presence of infection induced by intranasal inoculation of Mycobacterium avium in thalidomide-treated and untreated adult Swiss mice. Sixty animals were inoculated with 5 x 10(6) M. avium by the respiratory route. Thirty animals received daily thalidomide (30 mg/kg mouse) and 30 received water by gavage up to sacrifice. Ten non-inoculated mice were used as a control group. Lots of animals from each group were evaluated until 6 weeks after inoculation. Infection resulted in an increased total number of inflammatory cells as well...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium avium; Alveolar macrophages; Thalidomide; Experimental infection.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400005

The use of non-human primates as animal models for the study of hepatitis viruses
Provedor de dados: 56
Autores: Vitral,C.L.; Yoshida,C.F.T.; Gaspar,A.M.C.
Hepatitis viruses belong to different families and have in common a striking hepatotropism and restrictions for propagation in cell culture. The transmissibility of hepatitis is in great part limited to non-human primates. Enterically transmitted hepatitis viruses (hepatitis A virus and hepatitis E virus) can induce hepatitis in a number of Old World and New World monkey species, while the host range of non-human primates susceptible to hepatitis viruses transmitted by the parenteral route (hepatitis B virus, hepatitis C virus and hepatitis delta virus) is restricted to few species of Old World monkeys, especially the chimpanzee. Experimental studies on non-human primates have provided an invaluable source of information regarding the biology and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis viruses; Non-human primates; Animal model; Experimental infection.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800003

Antifungal activity of the naphthoquinone beta-lapachone against disseminated infection with Cryptococcus neoformans var. neoformans in dexamethasone-immunosuppressed Swiss mice
Provedor de dados: 56
Autores: Medeiros,C.S.; Pontes-Filho,N.T.; Camara,C.A.; Lima-Filho,J.V.; Oliveira,P.C.; Lemos,S.A.; Leal,A.F.G.; Brandão,J.O.C.; Neves,R.P.
The in vivo antifungal activity of the naphthoquinone beta-lapachone against disseminated infection by Cryptococcus neoformans was investigated. Swiss mice were immunosuppressed daily with dexamethasone (0.5 mg per mouse) intraperitoneally for 3 days, the procedure was repeated 4 days later, and the animals were then challenged intravenously with C. neoformans (10(6) CFU/mL) 1 week later. Seven days after infection, the mice were divided into groups and treated daily with beta-lapachone (10 mg/kg, iv) for 7 (N = 6) and 14 days (N = 10). Amphotericin B (0.5 mg/kg) was used as comparator drug and an additional group received PBS. Treatment with beta-lapachone cleared the yeast from the spleen and liver, and the fungal burden decreased approximately 10(4)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Beta-lapachone; Cryptococcus neoformans; Cryptococcosis; Immunesuppression; Experimental infection; Amphotericin B.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400004

Model of experimental infection in healthy and immunosuppressed swiss albino mice (Mus musculus) using Candida albicans strains with different patterns of enzymatic activity
Provedor de dados: 58
Autores: Chaves,Guilherme M.; Cavalcanti,Maria Auxiliadora de Q.; Carneiro-Leão,Ana Maria dos A.; Lopes,Silvia L.
With the purpose to evaluate Candida albicans virulence in vivo, two strains selected were based on their phospholipase and proteinase activity, and used in a model of experimental infection. One strain, isolated from vaginal secretion, was stocked at the URM Culture Collection for 43 years and presented high phospholipase (Pz=0.217) and proteinase (1.386 U.mL-1) activity. The other strain was a fresh strain isolated from oropharyngeal secretion of an AIDS patient, and presented low phospholipase (Pz=0.482) and proteinase (0.780 U.mL-1) activity. The strains were inoculated via intraperitoneum in immunosuppressed and non-immunosuppressed mice (Mus musculus) and the infection was evaluated over a period of 21 days. Liver, spleen, lungs and kidneys were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida albicans; Extracellular enzymes; Virulence; Experimental infection.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300010

Experimental infection with Brazilian Newcastle disease virus strain in pigeons and chickens
Provedor de dados: 58
Autores: Carrasco,Adriano de Oliveira Torres; Seki,Meire Christina; Benevenute,Jyan Lucas; Ikeda,Priscila; Pinto,Aramis Augusto.
Abstract This study was designed with the goal of adding as much information as possible about the role of pigeons (Columba livia) and chickens (Gallus gallus) in Newcastle disease virus epidemiology. These species were submitted to direct experimental infection with Newcastle disease virus to evaluate interspecies transmission and virus-host relationships. The results obtained in four experimental models were analyzed by hemagglutination inhibition and reverse transcriptase polymerase chain reaction for detection of virus shedding. These techniques revealed that both avian species, when previously immunized with a low pathogenic Newcastle disease virus strain (LaSota), developed high antibody titers that significantly reduced virus shedding after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallus gallus; Columba livia; Experimental infection; RT-PCR; Serology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100231

Experimental infection of horses with Vaccinia virus
Provedor de dados: 65
Autores: Barbosa,Claudio Henrique Gonçalves; Sant'Ana,Fabiano José Ferreira de; Cargnelutti,Juliana Felipetto; Flores,Eduardo Furtado; Teixeira Neto,Antônio Raphael; Santana,Rosália Bezerra de; Reis Junior,Janildo Ludolf.
ABSTRACT: Vaccinia virus (VACV) is the etiologic agent of bovine vaccinia, an emerging zoonotic disease with potential health issues for dairy herds and humans. VACV may occasionally infect other species, including horses. In this sense, an outbreak of VACV disease in horses was described in Pelotas, RS, in 2008, where a co-infection with two VACV strains (named Pelotas Virus 1 [P1V] and Pelotas Virus 2 [P2V]) was detected. Considering the rare occurrence of VACV infection in horses, the objective of this study was to investigate the susceptibility and pathogenesis of VACV infection in this species. Six adult horses were inoculated with VACV P1V or P2V (106.3TCID50/ml) through scarification of the nasolabial surface and monitored for virological and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: VACV; Experimental infection; Horses; Bovine vaccinia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300519

Clinical and virological characteristics of calves experimentally infected with a Brazilian isolate of bovine viral diarrhea virus type 1a
Provedor de dados: 65
Autores: Quadros,Luana Marchi; Jardim,José Conrado dos Santos; Merchioratto,Ingryd; Brum,Mário Celso Sperotto.
ABSTRACT: To study the pathogenicity of the Brazilian bovine viral diarrhea virus (BVDV) type 1a 241.10 isolate, four calves were intranasally inoculated with a viral suspension containing 107.2 TCID50 mL-1. One calf was left uninoculated and kept in contact with the other calves to investigate viral transmissibility. After inoculation, the animals were monitored daily for clinical signs of infection. The presence of the virus in the blood and nasal secretions was confirmed by virus isolation in cell culture. White blood cells were quantified prior to and every 3 days after infection, and the presence of antibodies was checked every 7 days, starting at day 0 until day 42 post-inoculation (pi). After infection, nasal and ocular serous secretions were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calf; Experimental infection; Pestivirus; Bovine; BVDV.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101986

Histopathological analysis of the reproductive system of male dogs experimentally infected with Toxoplasma gondii
Provedor de dados: 65
Autores: Arantes,Tiago Pereira; Lopes,Welber Daniel Zanetti; Ferreira,Roberta Machado; Pieroni,Juliana de Souza Pinto; Pinto,Vanessa Marigo Rocha; Santos,Thaís Rabelo dos; Sakamoto,Claúdio Alessandro; Costa,Alvimar José da.
The present research aimed to describe possible histopathological alterations in the reproductive system (testicles and epididymis) of male dogs experimentally infected with Toxoplasma gondii. Canines (n=10) serologically negative for T. gondii were selected and distributed into three experimental groups: GI, 3 inoculated with 2.0 x 10(5)P strain oocysts; GII, 3 infected with 1.0 x 10(6)RH strain tachyzoites; and GIII, 4 control dogs. Antibody research (IFAT) against T. gondii was realized. Toxoplasma gondii infection was confirmed by seroconversion of the 6 males infected with tachyzoites and oocysts from postinoculation day (PID) 7 and 14, respectively. At PID 70, all dogs were submitted to orchiectomy and testicle and epididymis samples were collected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine; Experimental infection; Toxoplasmosis; Histopathology; Testicle; Epididymis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000700026