Consulta: subjectFacets:"Extracellular DNA"
Registros recuperados: 2
Data/hora: 04/06/2024 01:38:45

Archaeal communities associated with shallow to deep subseafloor sediments of the New Caledonia Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Roussel, Erwan; Sauvadet, Anne-laure; Chaduteau, Carine; Fouquet, Yves; Charlou, Jean-luc; Prieur, Daniel; Cambon, Marie-anne.
P&gt;The distribution of the archaeal communities in deep subseafloor sediments [0-36 m below the seafloor (mbsf)] from the New Caledonia and Fairway Basins was investigated using DNA- and RNA-derived 16S rRNA clone libraries, functional genes and denaturing gradient gel electrophoresis (DGGE). A new method, Co-Migration DGGE (CM-DGGE), was developed to access selectively the active archaeal diversity. Prokaryotic cell abundances at the open-ocean sites were on average similar to 3.5 times lower than at a site under terrestrial influence. The sediment surface archaeal community (0-1.5 mbsf) was characterized by active Marine Group 1 (MG-1) Archaea that co-occurred with ammonia monooxygenase gene (amoA) sequences affiliated to a group of uncultured...
Tipo: Text
Palavras-chave: Marine subsurface sediments; Sea floor biosphere; Gradient gel electrophoresis; Microbial communities; Molecular diversity; Anaerobic oxidation; Sequence alignment; Phylogenetic trees; Extracellular DNA; Methane hydrate.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6801.pdf

An Assessment of Environmental Metabarcoding Protocols Aiming at Favoring Contemporary Biodiversity in Inventories of Deep-Sea Communities
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Brandt, Miriam; Trouche, Blandine; Henry, Nicolas; Liautard-haag, Cathy; Maignien, Lois; De Vargas, Colomban; Wincker, Patrick; Poulain, Julie; Zeppilli, Daniela; Arnaud-haond, Sophie.
The abyssal seafloor covers more than 50% of planet Earth and is a large reservoir of still mostly undescribed biodiversity. It is increasingly targeted by resource-extraction industries and yet is drastically understudied. In such remote and hard-to-access ecosystems, environmental DNA (eDNA) metabarcoding is a useful and efficient tool for studying biodiversity and implementing environmental impact assessments. Yet, eDNA analysis outcomes may be biased toward describing past rather than present communities as sediments contain both contemporary and ancient DNA. Using commercially available kits, we investigated the impacts of five molecular processing methods on eDNA metabarcoding biodiversity inventories targeting prokaryotes (16S), unicellular...
Tipo: Text
Palavras-chave: Environmental metabarcoding; RNA versus DNA; Extracellular DNA; Deep-sea biodiversity; Benthic ecology; Biomonitoring; Method testing.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00630/74165/73774.pdf