Consulta: subjectFacets:"Fluorescent in situ hybridization"
Registros recuperados: 9
Data/hora: 01/06/2024 18:39:33

A quantitative PCR approach for determining the ribosomal DNA copy number in the genome of Agave tequila Weber
Provedor de dados: Electronic Journal of Biotechnology
Autores: Rubio-Piña,Jorge; Quiroz-Moreno,Adriana; Sánchez-Teyer,L. Felipe.
Background: Agave tequilana has a great economic importance in Mexico in order to produce alcoholic beverages and bioenergy. However, in this species the structure and organization of the rDNAs in the genome are limited, and it represents an obstacle both in their genetic research and improvement as well. rDNA copy number variations per eukaryotic genome have been considered as a source of genetic rearrangements. In this study, the copy number of 18S and 5S rDNAs in the A. tequilana genome was estimated, and an absolute quantitative qPCR assay and genome size was used. In addition, an association between the rDNAs copy number and physical mapping was performed to confirm our results. Results: The analysis were successfully applied to determine copy number...
Tipo: Journal article
Palavras-chave: Agave tequilana; Copy number; Fluorescent in situ hybridization; Ribosomal DNA; Quantitative PCR.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400002

Effect of cycle time and airflow in biological nitrogen removal from poultry slaughterhouse wastewater using sequencing batch reactor
Provedor de dados: Engenharia Agrícola
Autores: Lopes,Carla L.; Mees,Juliana B. R.; Sene,Luciane; Carvalho,Karina Q. de; Christ,Divair; Gomes,Simone D.
This study aimed to evaluate the influence of airflow (0.25, 0.50 and 0.75 L.L-1.min-1) and cycle time (10.45 h, 14.25 h and 17.35 h) on a sequencing batch reactor (SBR) performance in promoting nitrification and denitrification of poultry slaughterhouse wastewater. The operational stages included feeding, aerobic and anoxic reactions, sedimentation and discharge. SBR was operated in a laboratory scale with a working volume of 4 L, keeping 25% of biomass retained inside the reactor as inoculum for the next batch. In the anoxic stage, C: N ratio was maintained between 5 and 6 by adding cassava starch wastewater. A factorial design (22) with five repetitions was designed at the central point to evaluate the influence of cycle time and airflow on total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitrification; Denitrification; Response surface methodology; Fluorescent in situ hybridization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162015000300567

Comparative analyses of three swallow species (Aves, Passeriformes, Hirundinidae): Insights on karyotype evolution and genomic organization
Provedor de dados: Genetics and Molecular Biology
Autores: Barcellos,Suziane Alves; Kretschmer,Rafael; Souza,Marcelo Santos de; Costa,Alice Lemos; Degrandi,Tiago Marafiga; Lopes,Cassiane Furlan; Ferguson-Smith,Malcolm A.; Pereira,Jorge; Oliveira,Edivaldo Herculano Correa de; Gunski,Ricardo José; Garnero,Analía del Valle.
Abstract Despite the richness of species in the Hirudinidae family, little is known about the genome organization of swallows. The Progne tapera species presents genetic and morphological difference when compared to other members of the same genus. Hence, the aims of this study were to analyze the chromosomal evolution of three species Progne tapera, Progne chalybea and Pygochelidon cyanoleuca - by comparative chromosome painting using two sets of probes, Gallus gallus and Zenaida auriculata, in order to determine chromosome homologies and the relationship between these species. All karyotypes exhibited 76 chromosomes with similar morphology, except for the 5th, 6th and 7th chromosome pairs in P. cyanoleuca. Additionally, comparative chromosome painting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homology; Molecular cytogenetics; Fluorescent in situ hybridization; Hirundinidae.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100201

A remarkable autosomal heteromorphism in Pseudoryzomys simplex 2n = 56; FN = 54-55 (Rodentia, Sigmodontinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Moreira,Camila Nascimento; Di-Nizo,Camilla Bruno; Silva,Maria José de Jesus; Yonenaga-Yassuda,Yatiyo; Ventura,Karen.
Pseudoryzomys simplex, the false rice rat, is a monotypic genus of the Oryzomyini tribe (Sigmodontinae) distributed in part of Bolivia, Paraguay, Argentina and Brazil. Its diploid number has been described as 56 acrocentric chromosomes decreasing in size and no karyotype figure has been depicted. Herein, we present karyotypic data on P. simplex, including chromosome banding and molecular fluorescent in situ hybridization using telomeric sequences and the whole X-chromosome of its sister clade Holochilus brasiliensis (HBR) as probes. A case of remarkable autosomal heteromorphism due to the presence of a whole heterochromatic arm leading to the variability of FN is reported, as well as the occurrence of regions of homology between the X and Y chromosomes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Fluorescent in situ hybridization; Heterochromatin; Oryzomyini.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200010

Chromosome divergence and NOR polymorphism in Bryconamericus aff. iheringii (Teleostei, Characidae) in the hydrographic systems of the Paranapanema and Ivaí Rivers, Paraná, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Capistano,Thiago Gomes; Castro,Ana Luiza de Brito Portela; Julio-Junior,Horácio Ferreira.
Cytogenetic studies were carried out in three populations of Bryconamericus aff. iheringii from two hydrographic systems of the Paranapanema and Ivaí Rivers, separated by a watershed, both belonging to the upper Paraná River basin. Specimens had a constant diploid number 2n = 52 chromosomes. However, three karyotype formulae were identified in the three populations: B. aff. iheringii from the Maringá stream had 12M+18SM+8ST+14A (FN = 90); specimens from Keller River showed 8M+28SM+6ST+10A (FN = 94) and specimens from the Tatupeba stream had 8M+20SM+8ST+16A (FN = 88). Nucleolar organizer regions (NORs) were identified by silver nitrate staining and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. Specimens from Tatupeba stream had a simple...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bryconamericus; Characid fish; Chromosome divergence; Fluorescent in situ hybridization; NOR polymorphism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200006

Karyotypic diversification in Mytilus mussels (Bivalvia: Mytilidae) inferred from chromosomal mapping of rRNA and histone gene clusters
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Perez Garcia, Maria Concepcion; Moran, Paloma; Pasantes, Juan J.
Background: Mussels of the genus Mytilus present morphologically similar karyotypes that are presumably conserved. The absence of chromosome painting probes in bivalves makes difficult verifying this hypothesis. In this context, we comparatively mapped ribosomal RNA and histone gene families on the chromosomes of Mytilus edulis, M. galloprovincialis, M. trossulus and M. californianus by fluorescent in situ hybridization (FISH). Results: Major rRNA, core and linker histone gene clusters mapped to different chromosome pairs in the four taxa. In contrast, minor rRNA gene clusters showed a different behavior. In all Mytilus two of the 5S rDNA clusters mapped to the same chromosome pair and one of them showed overlapping signals with those corresponding to one...
Tipo: Text
Palavras-chave: Mytilus; Chromosome; Fluorescent in situ hybridization; Histone genes; Ribosomal RNA genes; Fibre-FISH.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00204/31500/29906.pdf

Molecular detection of the oyster parasite Mikrocytos mackini, and a preliminary phylogenetic analysis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Carnegie, Ryan; Meyer, Gary; Blackbourn, Janice; Cochennec, Nathalie; Berthe, Franck; Bower, Susan.
The protistan parasite Mikrocytos mackini, the causative agent of Denman Island disease in the oyster Crassostrea gigas in British Columbia, Canada, is of wide concern because it can infect other oyster species and because its life cycle, mode of transmission, and origins are unknown. PCR and fluorescent in situ hybridization (FISH) assays were developed for M mackini, the PCR assay was validated against standard histopathological diagnosis, and a preliminary phylogenetic analysis of the M mackini small-subunit ribosomal RNA gene (SSU rDNA) was undertaken. A PCR designed specifically not to amplify host DNA generated a 544 bp SSU rDNA fragment from M mackini-infected oysters and enriched M. mackini cell isolates, but not from uninfected control oysters....
Tipo: Text
Palavras-chave: Fluorescent in situ hybridization; PCR validation; Mikrocytos mackini; Denman Island disease.
Ano: 2003
URL: https://archimer.ifremer.fr/doc/2003/publication-2814.pdf

Occurrence of Listeria monocytogenes in silages assessed by fluorescent in situ hybridization
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Oliveira,M.; Guerra,M.; Bernardo,F.
Avaliou-se a qualidade microbiológica da silagem produzida em Portugal e otimizaram-se alguns aspectos relacionados com a eficácia de detecção de Listeria monocytogenes mediante protocolo baseado na técnica de hibridação in situ fluorescente (FISH) para detecção directa desse microrganismo em amostras de silagens. O protocolo foi aplicado a 74 amostras, em simultâneo com o método bacteriológico convencional. Este último permitiu a detecção de L. monocytogenes em 11 silos (15%). Por meio do protocolo FISH, observou-se que 22 silos (29,7%) se encontravam contaminados. O método FISH apresentou precisão (77,0%), especificidade (92,5%) e sensibilidade (72,7%) elevadas, sendo adequado para a análise microbiológica presuntiva de silagens, uma vez que é um método...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silage; Listeriosis; Fluorescent in situ hybridization; Listeria monocytogenes; Portugal.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100038

Large scale distribution of bacterial communities in the upper Paraná River floodplain
Provedor de dados: Brazilian Journal of Microbiology
Autores: Chiaramonte,Josiane Barros; Roberto,Maria do Carmo; Pagioro,Thomaz Aurélio.
A bacterial community has a central role in nutrient cycle in aquatic habitats. Therefore, it is important to analyze how this community is distributed throughout different locations. Thirty-six different sites in the upper Paraná River floodplain were surveyed to determine the influence of environmental variable in bacterial community composition. The sites are classified as rivers, channels, and floodplain lakes connected or unconnected to the main river channel. The bacterial community structure was analyzed by fluorescent in situ hybridization (FISH) technique, based on frequency of the main domains Bacteria and Archaea, and subdivisions of the phylum Proteobacteria (Alpha-proteobacteria, Beta-proteobacteria, Gamma-proteobacteria) and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterioplankton distribution; Floodplain; Fluorescent in situ hybridization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400009