Consulta: subjectFacets:"Functional analysis"
Registros recuperados: 9
Data/hora: 04/06/2024 02:46:43

Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants
Provedor de dados: 74
Autores: Homrich,Milena Schenkel; Wiebke-Strohm,Beatriz; Weber,Ricardo Luís Mayer; Bodanese-Zanettini,Maria Helena.
Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenic plants; Particle bombardment; Agrobacterium systems; Functional analysis; Genetic improvement.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015

Functional abilities of cultivable plant growth promoting bacteria associated with wheat (Triticum aestivum L.) crops
Provedor de dados: 74
Autores: Moreira,Fernanda da S.; Costa,Pedro B. da; Souza,Rocheli de; Beneduzi,Anelise; Lisboa,Bruno B.; Vargas,Luciano K.; Passaglia,Luciane M. P.
Abstract In the pursuit of sustainable agriculture, bioinoculants usage as providers of a crop's needs is a method to limit environmental damage. In this study, a collection of cultivable putative plant growth promoting (PGP) bacteria associated with wheat crops was obtained and this bacterial sample was characterized in relation to the functional diversity of certain PGP features. The isolates were obtained through classical cultivation methods, identified by partial 16S rRNA gene sequencing and characterized for PGP traits of interest. Functional diversity characterization was performed using Categorical Principal Component Analysis (CatPCA) and Multiple Correspondence Analysis (MCA). The most abundant genera found among the 346 isolates were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant growth promoting bacteria; Categorical Principal Component Analysis; Functional analysis; Burkholderia genus.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100111

Some considerations for analyzing biodiversity using integrative metagenomics and gene networks
Provedor de dados: 5
Autores: Bittner, Lucie; Halary, Sebastien; Payri, Claude; Cruaud, Corinne; De Reviers, Bruno; Lopez, Philippe; Bapteste, Eric.
Background:  Improving knowledge of biodiversity will benefit conservation biology, enhance bioremediation studies, and could lead to new medical treatments. However there is no standard approach to estimate and to compare the diversity of different environments, or to study its past, and possibly, future evolution. Presentation of the hypothesis:  We argue that there are two conditions for significant progress in the identification and quantification of biodiversity. First, integrative metagenomic studies - aiming at the simultaneous examination (or even better at the integration) of observations about the elements, functions and evolutionary processes captured by the massive sequencing of multiple markers - should be preferred over DNA barcoding projects...
Tipo: Text
Palavras-chave: Microbial diversity; Species concept; Dna barcodes; Ecological perspective; Community; Functional analysis; Rhodophyta; Systematics; Fragments; Taxonomy.
Ano: 2010
URL: https://archimer.ifremer.fr/doc/00202/31321/29733.pdf

Aislamiento, clonaje y caracterización biológica del ADN  complementario de la Hormona Hiperglucemiante de Crustáceos de  Litopenaeus schmitti (Decapoda: Penaeidae)
Provedor de dados: 20
Autores: Yuliet Morera Darias.
El desarrollo alcanzado en la camaronicultura hasta el presente exige estudios cada vez más  novedosos en vista a enfrentar nuevos retos. En este sentido resulta necesario el conocimiento de  la estructura-función de los neuropéptidos de la familia de la Hormona Hiperglucemiante de  Crustáceos (familia HHC). Ella  juega un importante papel en el metabolismo y la fisiología  influyendo directamente sobre la muda, el crecimiento, la reproducción, el metabolismo de la  glucosa y lípidos, entre otras. La Hormona Hiperglucemiante de Crustáceos (HHC) es el  componente mayoritario de la familia HHC y el único neuropéptido aislado y secuenciado hasta  el presente. Ella es una hormona pleiotrópica y su función principal es regular el nivel de glucosa  en la...
Tipo: Theses and Dissertations
Palavras-chave: Marine shrimp culture; Anatomical structures; Functional analysis.
Ano: 2009
URL: http://hdl.handle.net/1834/5528

Recent developments in nucleic acid based techniques for use in rumen manipulation
Provedor de dados: 96
Autores: McSweeney,Christopher; Kang,Seungha; Gagen,Emma; Davis,Carl; Morrison,Mark; Denman,Stuart.
Nucleic acid-based techniques which can be used to characterise complex microbial communities without incubation are now being employed regularly in ruminant nutrition studies. Conventional culture-based methods for enumerating rumen microorganisms (bacteria, archaea, protozoa, and fungi) have been superseded and are now used mainly to obtain pure isolates of novel organisms and reference strains that are required for the development and validation of the nucleic acid approaches. These reference strains are also essential for physiological studies of the lifestyle of the organisms as well as sources of genomic DNA and RNA that can be analysed for functional gene activity. The foundation of the molecular ecology techniques is 16S/18S rDNA sequence analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecology; Functional analysis; Microbial; Molecular; Ribosomal; Rumen.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300034

Physical-chemical characterization of quinoa (Chenopodium quinoa Willd.), amaranth (Amaranthus caudatus L.), and chia (Salvia hispanica L.) flours and seeds
Provedor de dados: 109
Autores: García-Salcedo,Ángela Janet; Torres-Vargas,Olga Lucía; Ariza-Calderón,Hernando.
Abstract Quinoa, amaranth, and chia flours have considerable amounts of protein, starch, dietary fiber, lipids, minerals, vitamins, and bioactive components, which provide in them exceptional properties for human nutrition. The aim of this research was the physical-chemical characterization of quinoa, amaranth, and chia flours and seeds through proximal, optical, functional, thermal, and structural analyses. From the results obtained during the proximal analysis, considerable proportions were determined of protein and fiber for the three flours, with the highest values in chia flour at 28.56% and 39.8%, respectively. These results were corroborated by optical microscopy analysis performed on longitudinal cuts in the three types of seeds. Thermal parameters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioactive components; Functional analysis; Protein alternatives; Starch; Structural characterization.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000200215

Analysis of gene expression changes associated with human carcinoma-associated fibroblasts in non-small cell lung carcinoma
Provedor de dados: 48
Autores: Wu,Xiaofen; Ruan,Lei; Yang,Yi; Mei,Qi.
Abstract  Background  This study aimed to investigate the gene expression changes associated with carcinoma-associated fibroblasts (CAFs) involving in non-small cell lung carcinoma (NSCLC).  Methods  We downloaded the GEO series GSE22862, which contained matched gene expression values for 15 CAF and normal fibroblasts samples, and series GSE27289 containing SNP genotyping for four matched NSCLC samples. The differentially expressed genes in CAF samples were identified using the limma package in R. Then we performed gene ontology (GO) and pathway enrichment analysis and protein&amp;#8211;protein interaction (PPI) network construction using the identified DEGs. Moreover, aberrant cell fraction, ploidy, allele-specific copy number, and loss of heterozygosity...
Tipo: Journal article
Palavras-chave: Carcinoma-associated fibroblast; Non-small cell lung carcinoma; Differentially expressed genes; Allele-specific copy number; Functional analysis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100204

Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
Provedor de dados: 52
Autores: Santos,Neyliane Gonçalves dos; Amaral,Ademir; Cavalcanti,Mariana Brayner; Neves,Maria Amélia Batista; Machado,Cíntia Gonsalves de Faria.
The aim of this work was to establish a protocol to evaluate ionizing radiation effects on P-glycoprotein (P-gp) activity. For this, human peripheral blood samples were irradiated in vitro with different doses and P-gp activity was analyzed for CD4 and CD8 T lymphocytes through rhodamine123-efflux assay by flow cytometry. By simultaneous employment of percentage and mean fluorescence index parameters, subject-by-subject analysis pointed out changes in P-gp activity for some individuals and irradiated samples. Based on this work, the proposed protocol was considered adequate for evaluating P-gp activity on cells after radioactive stress. Besides, this research suggests that P-gp activity could be an important factor to define patient-specific protocols in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Combined therapy; Radiation effect; P-glycoprotein; Multidrug resistance; Functional analysis; Flow cytometry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700018

Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans
Provedor de dados: 58
Autores: Cheng,Jianhui; Wang,Hu; Xu,Xin; Wang,Liangyan; Tian,Bing; Hua,Yuejin.
Deinococcus radiodurans (DR) is an extremophile that is well known for its resistance to radiation, oxidants and desiccation. The gene dr1790 of D. radiodurans was predicted to encode a yellow-related protein. The primary objective of the present study was to characterize the biological function of the DR1790 protein, which is a member of the ancient yellow/major royal jelly (MRJ) protein family, in prokaryotes. Fluorescence labeling demonstrated that the yellow-related protein encoded by dr1790 is a membrane protein. The deletion of the dr1790 gene decreased the cell growth rate and sensitivity to hydrogen peroxide and radiation and increased the membrane permeability of D. radiodurans. Transcript profiling by microarray and RT-PCR analyses of the dr1790...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deinococcus radiodurans; Dr1790 disruptant; Characteristics; Functional analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200601