Consulta: subjectFacets:"GST"
Registros recuperados: 8
Data/hora: 04/06/2024 04:11:30

Population analysis of xenobiotic metabolizing genes in South Brazilian Euro and Afro-descendants
Provedor de dados: 74
Autores: Maciel,Marcos Euzébio; Oliveira,Fausto Koga; Propst,Gustavo Bonfim; Bicalho,Maria da Graça; Cavalli,Iglenir João; Ribeiro,Enilze Maria de Souza Fonseca.
Individual variability in xenobiotic metabolism has been associated with susceptibility to developing complex diseases. Genes involved in xenobiotic metabolism have been evaluated in association studies; the difficulty of obtaining accurate gene frequencies in mixed populations makes interpretation of the results difficult. We sought to estimate population parameters for the cytochrome P450 and glutathione S-transferase gene families, thus contributing to studies using these genes as markers. We describe the frequencies of six genes (CYP1A1, CYP2D6, CYP2E1, GSTM1, GSTT1, and GSTP1) and estimate population parameters in 115 Euro-descendants and 196 Afro-descendants from Curitiba, South of Brazil. PCR-based methods were used for genotyping, and statistical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYP; GST; Population study.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400010

Multiple biomarkers of pollution effects in caged mussels on the Greek coastline
Provedor de dados: 5
Autores: Tsangaris, C.; Kormas, K.; Strogyloudi, E.; Hatzianestis, I.; Neofitou, C.; Andral, Bruno; Galgani, Francois.
A suite of biomarkers was measured in caged mussels at areas impacted by different anthropogenic activities along the Greek coastline to assess biological effects of environmental pollution. Mussels were caged at coastal sites in the vicinity of major cities, in areas influenced by major industries, agricultural practices and in islands away from known sources of pollution. Biomarkers indicative of neurotoxicity (acetylcholinesterase, AchE), oxidative stress (catalase, CAT), phase 11 biotransformation of xenobiotics (glutathione S-transferase, GST), metal exposure (metallothioneins, MTs) and protein synthesis (RNA:DNA ratio) were measured to assess effects of various types of pollutants. AchE activity proved to be the most responsive biomarker with...
Tipo: Text
Palavras-chave: Biomarkers; AchE; CAT; GST; MTS; RNA:DNA ratio; Mytilus galloprovincialis; Mediterranean.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11286/7890.pdf

Bioindicators of pollutant exposure in the northwestern Mediterranean sea
Provedor de dados: 5
Autores: Burgeot, Thierry; Bocquene, Gilles; Porte, C; Dimeet, Joel; Santella, Rm; Garcia De La Parra, L.m.; Pihol-leszkowicz, A.; Raoux, C.; Galgani, Francois.
Several bioindicators were used to evaluate the biological and genotoxic effects of marine pollutants near large coastal cities in the northwestern part of the Mediterranean Sea. Three target species of teleosts were selected: red mullet Mullus barbatus and 2 types of comber (Serranus hepatus and S. cabrilla). Induction of ethoxyresorufin-O-deethylase (EROD) activity specific for polycyclic aromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCB) was measured in the livers of the fish, and inhibition of acetylcholinesterase (AChE) by organophosphorus insecticides and carbamates was measured in their muscle tissues. Maximal EROD activities (16.8 +/- 2.7 to 19.4 +/- 4.2 pmol min(-1) mg protein(-1)) recorded in red muller near Barcelona (Spain),...
Tipo: Text
Palavras-chave: EROD; GST; AChE; Antioxidant enzymes; Biomonitoring; DNA adducts; PAH; Mullus barbatus.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00183/29410/27779.pdf

Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus )
Provedor de dados: 83
Autores: Copatti,Carlos Eduardo; Baldisserotto,Bernardo; Souza,Carine de Freitas; Monserrat,José María; Garcia,Luciano.
ABSTRACT This study aimed to assess the effects of low and high water hardness in interaction with different water pH in pacu (Piaractus mesopotamicus). Pacu juveniles were subjected to low (50 mg CaCO3 L-1 - LWH) or high water hardness (120 mg CaCO3 L-1 - HWH) at water pH of 5.5 (acidic), 7.5 (circumneutral) or 9.0 (alkaline) for 15 days. Gills and kidneys were collected (days 1, 5 and 15). Gill Na+/K+-ATPase (NKA) and vacuolar-type H+-ATPase (V-ATPase) activities were higher in alkaline pH with HWH on day 1. Gill and kidney NKA and V-ATPase activities were higher in acidic pH with LWH on day 15. Gill NKA activity of pacus under alkaline pH with LWH was higher than those exposed to HWH. Reduced antioxidant capacity in the gills and kidney and enhanced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acidic pH; Alkaline pH; GST; Na+/K+-ATPase; TBARS.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500204

Oxidative stress parameters in juvenile Brazilian flounder Paralichthys orbignyanus (Valenciennes, 1839) (Pleuronectiformes: Paralichthyidae) exposed to cold and heat shocks
Provedor de dados: 83
Autores: Garcia,Luciano de O.; Okamoto,Marcelo H.; Riffel,Ana Paula K.; Saccol,Etiane M.; Pavanato,Maria A.; Sampaio,Luís André N.
The aim of this study was to determine oxidative stress parameters in the liver and gill of Brazilian flounder juveniles (307.0 ± 16.0 g and 30.0 ± 4.0 cm) submitted to different water temperature (17.1, 23.0 and 28.8ºC) for 72 h and maintained at salinity 25‰. After the acclimation of 7 days, in 23ºC, fish were transferred to 200 L tanks containing seawater (salinity 25‰) at 28.8ºC (heat shock), 17.1ºC (cold shock) or 23.0ºC (control), five replicates (five fish tank-1). The sampled collection occurred in 0 (pre-challenge), 3, 24, 48 and 72 h after temperature shock. Flounder exposed to 17.1ºC and 28.8ºC showed significantly higher TBARS levels and GST activity in the liver post-exposition (PE) in relation to the control (23ºC). CAT activity in liver...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; GST; Lipid peroxidation; TBARS; Thermal shocks.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300607

CONSUMER AND IMPORT TAXES IN THE WORLD WINE MARKET: AUSTRALIA IN INTERNATIONAL PERSPECTIVE
Provedor de dados: 31
Autores: Berger,   Nicholas; Anderson,   Kym.
Virtually all countries tax the consumption of wine (and other alcoholic beverages). However, the rates of taxation, and the tax instruments used, vary enormously between countries. This paper details for all OECD and some other countries the consumer tax rates as of 1996, showing specific or ad valorem excise or wholesale sales taxes, import tariffs, export subsidies and value-added or goods-and-services taxes. It also aggregates them into an ad valorem consumer tax equivalent (CTE) at various wine price levels (since many are specific taxes and so their CTE varies with the price). T he consumer tax equivalent tends to be lower the greater a country's per capita production of wine, especially for premium wine. Australia and New Zealand are shown to have...
Tipo: Presentation
Palavras-chave: Consumer wine taxation; GST; Excise taxes; Consumer/Household Economics; H21; H22; H23; F13.
Ano: 1999
URL: http://purl.umn.edu/123770

Oxidative stress responses of juvenile tambaqui Colossoma macropomum after short-term anesthesia with benzocaine and MS-222
Provedor de dados: 42
Autores: STRINGHETTA,GIOVANNA R.; BARBAS,LUIS A.L.; MALTEZ,LUCAS C.; SAMPAIO,LUÍS A.; MONSERRAT,JOSÉ M.; GARCIA,LUCIANO O.
ABSTRACT The present study aimed to evaluate the effects of benzocaine and tricaine methanesulfonate on oxidative stress parameters of juvenile tambaqui tissues. Fish (n=80) were anesthetized with benzocaine (100 mg L-1) or tricaine (240 mg L-1) and two control groups were used (non-anesthetized fish and fish exposed to ethanol-only). After anesthetic induction 10 fish/anesthetic were euthanized after 3, 12 and 24 hours post-anesthesia and tissue samplings (gills, liver and brain) were performed. Samples were submitted to analyses of enzyme activity glutathione-S-transferase (GST), cellular lipid peroxidation (TBARS) and total antioxidant capacity (ACAP). ACAP increased in gills of benzocaine treatment after 12 hours. The liver showed a reduction in ACAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anesthetics; Fish; GST; Lipid peroxidation; Total antioxidant capacity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502209

Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis
Provedor de dados: 56
Autores: Sato,N.S.; Suzuki,T.; Ueda,T.; Watanabe,K.; Hirata,R.D.C.; Hirata,M.H.
Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purified fusion protein yields were 1.9, 1.7 and 5.3 mg/l of cell culture for GST-rTp47, GST-rTp17 and GST-rTp15, respectively. The identities of the antigens obtained were confirmed by automated DNA sequencing using ABI Prism 310 and peptide mapping by Finningan LC/MS. These recombinant antigens were evaluated by immuno-slot blot techniques applied to 137 serum samples from patients with a clinical and laboratory diagnosis of syphilis (61 samples), from healthy...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Treponema pallidum; Cloning; Recombinant antigen; Syphilis; GST.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700002