Consulta: subjectFacets:"GUS"
Registros recuperados: 5
Data/hora: 30/05/2024 11:46:48

Acetosyringone, pH and temperature effects on transient genetic transformation of immature embryos of Brazilian wheat genotypes by Agrobacterium tumefaciens
Provedor de dados: 74
Autores: Manfroi,Ernandes; Yamazaki-Lau,Elene; Grando,Magali F.; Roesler,Eduardo A.
Abstract Low transformation efficiency is one of the main limiting factors in the establishment of genetic transformation of wheat via Agrobacterium tumefaciens. To determine more favorable conditions for T-DNA delivery and explant regeneration after infection, this study investigated combinations of acetosyringone concentration and pH variation in the inoculation and co-cultivation media and co-culture temperatures using immature embryos from two Brazilian genotypes (BR 18 Terena and PF 020037). Based on transient expression of uidA, the most favorable conditions for T-DNA delivery were culture media with pH 5.0 and 5.4 combined with co-culture temperatures of 22 °C and 25 °C, and a 400 μM acetosyringone supplement. These conditions resulted in blue foci...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-cultivation conditions; Protocol establishment; GUS; T-DNA delivery.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400470

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency
Provedor de dados: 74
Autores: Carvalho,Carlos Henrique S.; Zehr,Usha B.; Gunaratna,Nilupa; Anderson,Joseph; Kononowicz,Halina H.; Hodges,Thomas K.; Axtell,John D.
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agrobacterium tumefaciens; GUS; Transformation; Sorghum.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200022

Sorção, dessorção e potencial de lixiviação de dimethenamid em solos brasileiros
Provedor de dados: 87
Autores: Archangelo,E.R.; Karam,D.; Ferreira,F.A.; Prates,H.T.; Ferreira,L.R.; Cardoso,A.A.
Determinar a sorção, a dessorção e o potencial de lixiviação do dimethenamid em diferentes solos foi o objetivo deste trabalho. As amostras dos solos foram agitadas por 24 horas e centrifugadas, sendo o sobrenadante filtrado e analisado por HPLC. As etapas da dessorção foram conduzidas por meio da reposição do sobrenadante removido com o mesmo volume de solução de 0,01 M de CaCl2 com agitação por 24 h e posterior centrifugação, repetindo-se o processo por três vezes consecutivas (24, 48 e 72 h) sendo o sobrenadante filtrado e analisado por HPLC. A sorção do dimethenamid, descrita pela isoterma de Freundlich, foi calculada pela diferença entre as concentrações adicionadas e aquelas quantificadas no HPLC. Foram calculadas as percentagens de dessorção a 24,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoterma de Freundlich; Kf; Kfoc; GUS; Herbicida.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582004000300018

PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus
Provedor de dados: 94
Autores: MIYATA,LUZIA YURIKO; HARAKAVA,RICARDO; ATTÍLIO,LÍSIA BORGES; MENDES,BEATRIZ MADALENA JANUZZI; LOPES,JOÃO ROBERTO SPOTTI; COLETTA-FILHO,HELVÉCIO DELLA; SOUZA,ALESSANDRA ALVES DE; MOURÃO FILHO,FRANCISCO DE ASSIS ALVES.
ABSTRACT  The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 ( pCitrus phloemrotein 2), known to be expressed in Citrus sinensis phloem. ‘Hamlin’ sweet orange plants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus sinensis; Diaphorina citri; Genetic transformation; GUS; Huanglongbing; Quantitative real-time PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000300101

Chloroplast localization of Cry1Ac and Cry2A protein- an alternative way of insect control in cotton
Provedor de dados: 48
Autores: Muzaffar,Adnan; Kiani,Sarfraz; Khan,Muhammad Azmat Ullah; Rao,Abdul Qayyum; Ali,Arfan; Awan,Mudassar Fareed; Iqbal,Adnan; Nasir,Idrees Ahmad; Shahid,Ahmad Ali; Husnain,Tayyab.
BACKGROUND: Insects have developed resistance against Bt-transgenic plants. A multi-barrier defense system to weaken their resistance development is now necessary. One such approach is to use fusion protein genes to increase resistance in plants by introducing more Bt genes in combination. The locating the target protein at the point of insect attack will be more effective. It will not mean that the non-green parts of the plants are free of toxic proteins, but it will inflict more damage on the insects because they are at maximum activity in the green parts of plants. RESULTS: Successful cloning was achieved by the amplification of Cry2A, Cry1Ac, and a transit peptide. The appropriate polymerase chain reaction amplification and digested products confirmed...
Tipo: Journal article
Palavras-chave: Chloroplast transient peptide; Cry1Ac; Cry2A; Bt; CTP; Cry genes; Endotoxins; GUS; Transgenic plants.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100014