Consulta: subjectFacets:"Gene structure"
Registros recuperados: 4
Data/hora: 10/06/2024 09:59:11

Diversity and evolution of plant diacylglycerol acyltransferase (DGATs) unveiled by phylogenetic, gene structure and expression analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Turchetto-Zolet,Andreia Carina; Christoff,Ana Paula; Kulcheski,Franceli Rodrigues; Loss-Morais,Guilherme; Margis,Rogerio; Margis-Pinheiro,Marcia.
Abstract Since the first diacylglycerol acyltransferase (DGAT) gene was characterized in plants, a number of studies have focused on understanding the role of DGAT activity in plant triacylglycerol (TAG) biosynthesis. DGAT enzyme is essential in controlling TAGs synthesis and is encoded by different genes. DGAT1 and DGAT2 are the two major types of DGATs and have been well characterized in many plants. On the other hand, the DGAT3 and WS/DGAT have received less attention. In this study, we present the first general view of the presence of putative DGAT3 and WS/DGAT in several plant species and report on the diversity and evolution of these genes and its relationships with the two main DGAT genes (DGAT1 and DGAT2). According to our analyses DGAT1, DGAT2,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triacylglycerol biosynthesis; DGAT; Phylogeny; Gene structure.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400524

Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species
Provedor de dados: Genetics and Molecular Biology
Autores: Wasko,Adriane P.; Severino,Fábio E.; Presti,Flávia T.; Poletto,Andréia B.; Martins,Cesar.
An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actin gene; Amino acid residues; Gene structure; Nile tilapia; Nucleotide sequence; Oreochromis niloticus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600010

Structure analysis of the endoxylanase A gene from Penicillium purpurogenum
Provedor de dados: Biological Research
Autores: CHÁVEZ,RENATO; ALMARZA,CAROLINA; SCHACHTER,KAREN; PEIRANO,ALESSANDRA; BULL,PAULINA; EYZAGUIRRE,JAIME.
Penicillium purpurogenum produces several endoxylanases, two of which (XynA and XynB) have been purified and characterized. XynB has been sequenced, and it belongs to glycosyl hydrolase family 11. In this publication we report the structure of the xynA gene. The amino terminal sequence of the protein was determined and this allowed the design of oligonucleotides for use in polymerase chain reactions. Different polymerase chain reaction strategies were used to amplify and sequence the entire cDNA and the gene. The gene has an open reading frame of 1450 base pairs, including 8 introns with an average length of 56 base pairs each. Only one copy of this gene is present in the P. purpurogenum genome as shown by Southern blot. The gene encodes a protein of 329...
Tipo: Journal article
Palavras-chave: Family 10 endoxylanases; Gene structure; Penicillium purpurogenum.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300009

Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride
Provedor de dados: Brazilian Journal of Microbiology
Autores: Matroudi,S.; Zamani,M.R.; Motallebi,M.
In this study Trichoderma atroviride was selected as over producer of chitinase enzyme among 30 different isolates of Trichoderma sp. on the basis of chitinase specific activity. From this isolate the genomic and cDNA clones encoding chit33 have been isolated and sequenced. Comparison of genomic and cDNA sequences for defining gene structure indicates that this gene contains three short introns and also an open reading frame coding for a protein of 321 amino acids. The deduced amino acid sequence includes a 19 aa putative signal peptide. Homology between this sequence and other reported Trichoderma Chit33 proteins are discussed. The coding sequence of chit33 gene was cloned in pEt26b(+) expression vector and expressed in E. coli.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichoderma atroviride; Chit33; Chitinase activity; Gene structure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300005