Consulta: subjectFacets:"Genetic variability"
Registros recuperados: 158
Data/hora: 11/06/2024 06:24:36

Diversidad de maíces nativos de tres nichos ecológicos del altiplano poblano-tlaxcalteca.
Provedor de dados: 32
Autores: Alvarado Beltrán, Gregorio.
Con la finalidad de evaluar el nivel de diversidad morfológica y genética de maíces nativos de los nichos ecológicos de Libres, Mazapiltepec y Huamantla, correspondientes a los estados de Puebla y Tlaxcala, se desarrolló la presente investigación, la cual se dividió en dos etapas. La primera comprendió una caracterización morfológica, evaluando 134 poblaciones nativas de la región de estudio, siete testigos raciales y tres testigos comerciales, en un diseño experimental látice simple 12 x 12 con dos repeticiones en tres localidades, registrándose 21 caracteres morfológicos. La segunda consistió en un análisis isoenzimático en 49 poblaciones nativas representativas y cinco testigos raciales, explorando diez sistemas enzimáticos que codifican para 19 loci....
Palavras-chave: Zea mays L.; Poblaciones nativas; Recursos fitogenéticos; Variabilidad genética; Landraces; Plant genetic resources; Genetic variability; Doctorado; Genética.
Ano: 2010
URL: http://hdl.handle.net/10521/182

Identificación y fluctuación poblacional de trips (Insecta: Thysanoptera) asociados con hortalizas de la región central de México.
Provedor de dados: 32
Autores: Turcios Palomo, Ciro Luis Antonio.
En la actualidad los cultivos de hortalizas en México son severamente afectados por  insectos conocidos como trips (Thysanoptera: Insecta), las pérdidas son notables en jitomate, chile, tomate de cáscara, calabacita, pepino y cebolla por la importancia económica que representan. La afectación es, por los  daños que ocasionan cuando se alimentan de sus brotes y frutos tiernos, follaje y flores, por la transmisión de virus fitopatógenos, por su periodicidad  y por las constantes aplicaciones de productos fitosanitarios para su control. Las especies Frankliniella occidentalis y Thrips tabaci, destacan como las más relevantes en estos cultivos por su capacidad invasiva, su amplia distribución y polifagia. De febrero de 2010 a febrero de 2011 se muestrearon...
Palavras-chave: Frankliniella; Thrips; Fluctuación poblacional; Variabilidad genética; Population fluctuation; Genetic variability; Entomología y Acarología; Doctorado.
Ano: 2013
URL: http://hdl.handle.net/10521/2184

Diversidad de maíces nativos de tres nichos ecológicos del altiplano poblano-tlaxcalteca.
Provedor de dados: 32
Autores: Alvarado Beltrán, Gregorio.
Con la finalidad de evaluar el nivel de diversidad morfológica y genética de maíces nativos de los nichos ecológicos de Libres, Mazapiltepec y Huamantla, correspondientes a los estados de Puebla y Tlaxcala, se desarrolló la presente investigación, la cual se dividió en dos etapas. La primera comprendió una caracterización morfológica, evaluando 134 poblaciones nativas de la región de estudio, siete testigos raciales y tres testigos comerciales, en un diseño experimental látice simple 12 x 12 con dos repeticiones en tres localidades, registrándose 21 caracteres morfológicos. La segunda consistió en un análisis isoenzimático en 49 poblaciones nativas representativas y cinco testigos raciales, explorando diez sistemas enzimáticos que codifican para 19 loci....
Palavras-chave: Zea mays L.; Poblaciones nativas; Recursos fitogenéticos; Variabilidad genética; Landraces; Plant genetic resources; Genetic variability; Doctorado; Genética.
Ano: 2010
URL: http://hdl.handle.net/10521/182

Evolución genética de cuatro especies de Anastrepha spp. (Díptera: Tephritidae).
Provedor de dados: 32
Autores: Teodoro Pardo, Claudia Verónica de.
El objetivo de este estudio fue analizar la variabilidad genética en diferentes especies del género Anastrepha (Anastrepha ludens, A. obliqua, A. serpentina y A. striata), estudiar el flujo genético en A. ludens entre diferentes poblaciones de México y de Guatemala mediante un análisis isoenzimático, cuantificar ADN del ganglio cerebral y glándulas salivales y explorar la hibridación genómica in situ (GISH) en cada una de las especies de moscas en condiciones de laboratorio. Se analizaron los sistemas isoenzimáticos 6PGDH, IDH, ME, MDH, GPI y GOT en las cuatro especies de moscas en condiciones de laboratorio y campo, y para el flujo genético sólo cuatro sistemas fueron estudiados. Se examinaron 30 individuos (15 hembras y 15 machos) de cada población. Los...
Palavras-chave: Anastrepha; Contenido de ADN; Citometría de flujo; Electroforesis; GISH; Isoenzimas; Flujo genético; Variabilidad genética; DNA content; Flow cytometry; Electrophoresis; Isozymes; Gene flow; Genetic variability; Doctorado; Genética.
Ano: 2011
URL: http://hdl.handle.net/10521/569

Evolución genética de cuatro especies de Anastrepha spp. (Díptera: Tephritidae).
Provedor de dados: 32
Autores: Teodoro Pardo, Claudia Verónica de.
El objetivo de este estudio fue analizar la variabilidad genética en diferentes especies del género Anastrepha (Anastrepha ludens, A. obliqua, A. serpentina y A. striata), estudiar el flujo genético en A. ludens entre diferentes poblaciones de México y de Guatemala mediante un análisis isoenzimático, cuantificar ADN del ganglio cerebral y glándulas salivales y explorar la hibridación genómica in situ (GISH) en cada una de las especies de moscas en condiciones de laboratorio. Se analizaron los sistemas isoenzimáticos 6PGDH, IDH, ME, MDH, GPI y GOT en las cuatro especies de moscas en condiciones de laboratorio y campo, y para el flujo genético sólo cuatro sistemas fueron estudiados. Se examinaron 30 individuos (15 hembras y 15 machos) de cada población. Los...
Palavras-chave: Anastrepha; Contenido de ADN; Citometría de flujo; Electroforesis; GISH; Isoenzimas; Flujo genético; Variabilidad genética; DNA content; Flow cytometry; Electrophoresis; Isozymes; Gene flow; Genetic variability; Doctorado; Genética.
Ano: 2011
URL: http://hdl.handle.net/10521/569

Analysis of diversity among six populations of Colombian mango (Mangifera indica L. cvar. Hilacha) using RAPDs markers
Provedor de dados: 69
Autores: Díaz-Matallana,Marcela; Schuler-García,Ingrid; Ruiz-García,Manuel; Hodson de Jaramillo,Elizabeth.
Currently in Colombia, there are only records of morph-agronomic characterizations of Mangifera indica cvar. Hilacha; molecular studies on this mango variety have not been carried out. The aim of this work was to identify the genetic diversity of six populations of mango Hilacha by RAPDs markers, as a fundamental base for breeding programs, conservation and selection of promissory materials for the fruit industry at the national level. From 60 primers evaluated in the populations, five primers were selected and were launched in the six populations. Polymorphic bands of RAPDs were transformed into binary matrices, which were then processed with NTSYS-PC, POPGENE and TFPGA softwares. The overall genetic diversity, H T = 0.468 ± 0.0016, is very similar to the...
Tipo: Journal article
Palavras-chave: Genetic variability; Mango Hilacha; Organoleptic quality; RAPDs.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300001

Genetic diversity of breeding popcorn lines determined by SSR markers
Provedor de dados: 69
Autores: Ribeiro Trindade,Ana Paula; Barth Pinto,Ronald José; Amaral Júnior,Antonio Teixeira do; Mangolin,Claudete Aparecida; Silva Machado,Maria de Fátima Pires da; Scapim,Carlos Alberto.
Information about genetic dissimilarity is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations. Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers. Of a total of 51 evaluated polymorphic primers, 15 were used for polymerase chain reaction (PCR) amplification. The genetic distance was estimated by Rogers&amp;#8217; modified distance. The different popcorn breeding programs in Brazil are possibly using highly similar base-populations. The genetic similarity of lines P1-3 and P8-1 was lowest, while P3-3 and P8-2 were genetically more similar. The cophenetic correlation showed that the Unweighted Pair-Group Method Using Arithmetic Averages...
Tipo: Journal article
Palavras-chave: Advanced generations; Genetic variability; Molecular markers; Zea mays.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100004

Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markers
Provedor de dados: 69
Autores: da Silva,Tereza Aparecida; Belizário Cantagalli,Liriana; Saavedra,Javier; Lopes,Ana Daniela; Aparecida Mangolin,Claudete; Pires da Silva Machado,Maria de Fatima; Scapim,Carlos Alberto.
Background The genetic diversity and structure of 31 popcorn accessions of the germplasm bank of the State University of Maringá were assessed using 30 microsatellite primers. Results 127 alleles were identified from 30 evaluated loci. The number of alleles per locus ranged from two to eight. The overall mean of the polymorphic loci averaged 79.89%. The primers UMC1549 and UMC1072 detected polymorphism in all accessions analyzed. The mean observed heterozygosity ranged from 0.07 to 0.30 and the highest proportion of heterozygous plants was observed in accession BOZM 260 (Ho = 0.30). The analysis of molecular variance revealed that 60% of the total genetic variation was found within accessions and 40% was found between accessions. The Bayesian clustering...
Tipo: Journal article
Palavras-chave: Genetic variability; Maize; Molecular marker.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300007

Genetic diversity analysis of traditional and improved cultivars of Pakistani rice (Oryza sativa L.) using RAPD markers
Provedor de dados: 69
Autores: Rabbani,Malik Ashiq; Pervaiz,Zahida Hassan; Masood,Muhammad Shahid.
The molecular marker is a useful tool for assessing genetic variations and resolving cultivar identities. Information on genetic diversity and relationships among rice genotypes from Pakistan is currently very limited. The objective of this study was to evaluate the genetic polymorphisms and identities of 10 traditional, 28 improved and 2 Japanese cultivars of rice using the random amplified polymorphic DNA technique. Twenty-five decamer-primers could generate a total of 208 RAPD fragments, of which 186 or 89.4% were polymorphic. The number of amplification products produced by each primer varied from 4 to 16 with an average of 8.3 bands primer-1. The size of amplified fragments were ranged from 200 to 4000 bp. Pair-wise Nei and Li's similarity had...
Tipo: Journal article
Palavras-chave: Cultivar discrimination; Genetic variability; Oryza sativa; Pakistani rice; RAPDs.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300006

WITHIN-POPULATION GENETIC DIVERSITY OF CLIMBING PLANTS AND TREES IN A TEMPERATE FOREST IN CENTRAL CHILE
Provedor de dados: 73
Autores: Torres-Díaz,Cristian; Ruiz,Eduardo; Salgado-Luarte,Cristian; Molina-Montenegro,Marco A; Gianoli,Ernesto.
The climbing habit is a key innovation in angiosperm evolution: climbing plant taxa have greater species richness than their non-climbing sister groups. It is considered that highly diversified clades should show increased among-population genetic differentiation. Less clear is the expected pattern regarding within-population genetic diversity in speciose lineages. We tested the hypothesis of greater within-population genetic diversity in climbing plants compared to trees in a temperate forest in central Chile. The assumption underlying this hypothesis is that higher among-population differentiation in climbers compared to trees should reflect higher genetic diversity as well. AFLP markers from 167 individual plants from 14 species (seven climbers and...
Tipo: Journal article
Palavras-chave: AFLP; Climbers; Evolution; Genetic variability; Key innovation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432013000100005

Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
Provedor de dados: 74
Autores: Silveira,Sheila Recepute; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Sera,Tumoru; Carvalho,Valdemar de Paula; Coelho,Alexandre Siqueira Guedes.
The RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67%) were polymorphic. AMOVA showed that 38.5% and 61.5% of the genetic variation was distributed among and within populations, respectively. The fixation index (F ST) of the genotypes was 0.385....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee breeding; Genetic variability; Molecular variance; RAPD markers.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300018

Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers
Provedor de dados: 74
Autores: Veasey,Elizabeth Ann; Borges,Aline; Rosa,Mariana Silva; Queiroz-Silva,Jurema R.; Bressan,Eduardo de Andrade; Peroni,Nivaldo.
We used simple sequence repeat (SSR) markers to investigate the genetic diversity of 78 sweet potato (Ipomoea batatas) accessions (58 landraces and 20 putative clones) from traditional agricultural households from 19 local communities in the Vale do Ribeira, São Paulo, Brazil. Eight SSR loci were assessed using 6% (w/v) polyacrylamide gels stained with silver nitrate and the accessions genotyped considering the presence or absence of bands. The results were subjected to analysis of molecular variance (AMOVA), and cluster and principal coordinate analyses. Spatial structure was assessed using Mantel's test to compare genetic and geographic distances. Each primer pair generated between three and ten clearly scorable polymorphic fragments. Cluster analyses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Landraces; SSR; Traditional agriculture; Vale do Ribeira.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400020

High levels of genetic variability and differentiation in hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) populations revealed by PCR-RFLP analysis of the mitochondrial DNA D-loop region
Provedor de dados: 74
Autores: Mazumder,Sabuj Kanti; Alam,Md. Samsul.
The hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) is an important anadromous clupeid species from the Western division of the Indo-Pacific region. It constitutes the largest single fishable species in Bangladesh. Information on genetic variability and population structure is very important for both management and conservation purposes. Past reports on the population structure of T. ilisha involving morphometric, allozyme and RAPD analyses are contradictory. We examined genetic variability and divergence in two riverine (the Jamuna and the Meghna), two estuarine (Kuakata and Sundarbans) and one marine (Cox's Bazar) populations of T. ilisha by applying PCR-RFLP analysis of the mtDNA D-loop region. The amplified PCR products were restricted with four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; PCR-RFLP; MtDNA; Tenualosa ilisha.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100029

Genetic variability in the endophytic fungus Guignardia citricarpa isolated from citrus plants
Provedor de dados: 74
Autores: Glienke-Blanco,Chirlei; Aguilar-Vildoso,Carlos Ivan; Vieira,Maria Lúcia Carneiro; Barroso,Paulo Augusto Vianna; Azevedo,João Lúcio.
During some phases of of their life-cycle endophytic fungi colonize plants asymptomatically being found most frequently inside the aerial part of plant tissues. After surface disinfection of apparently healthy leaves from three varieties of mandarin orange and one tangor, and after incubation on appropriate culture medium, 407 fungal isolates were obtained, giving a total infection frequency of 81%. No fungal growth was observed from disinfected seeds, indicating that fungi are probably not transmitted via seeds. Of the fungal isolates, 27% belonged to the genus Guignardia, with 12 isolates being identified as Guignardia citricarpa Kiely, which is described as a citrus pathogen. The isolates were variable in respect to the presence of sexual structures and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Endophytes; Genetic variability; Guignardia citricarpa; Mandarin; RAPD.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200021

Genetic characterization of 18 novel microsatellite loci in northern pike (Esox lucius L.)
Provedor de dados: 74
Autores: Wang,Jun; Wang,Chenghui; Qian,Long; Ma,Yuqing; Yang,Xinxin; Jeney,Zsigmond; Li,Sifa.
The northern pike (Esox lucius L.), an important predatory freshwater species, is undergoing significant population decline. In this study, 18 novel polymorphic microsatellite loci were isolated and used for assessing genetic variation in the Chinese Ulungur and Hungarian Balaton populations of the species. The number of alleles ranged from 2 to 13, observed heterozygosity from 0.154 to 0.920 and expected heterozygosity from 0.145 to 0.921, thereby indicating the specific usefulness of these suites of markers for investigating genetic variability.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Northern pike; Microsatellite loci; Genetic variability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100029

Molecular characterization of  Chaetomium  species using URP-PCR
Provedor de dados: 74
Autores: Aggarwal,Rashmi; Sharma,Vandana; Kharbikar,Lalit L.; Renu,.
Chaetomium  spp. are common colonizers of soil and cellulose-containing substrates. Seventeen isolates of  Chaetomium  spp., which included 15 isolates of  C. globosum  and one each of  C. reflexum  and  C. perlucidum , were genetically characterized with universal rice primers (URP - primers derived from DNA repeat sequences in the rice genome) using polymerase chain reaction (URP-PCR). Out of the 12 URP's used in the study, nine primers were effective in producing polymorphic fingerprint patterns from DNA of  Chaetomium spp . Analysis of the entire fingerprint profile using the unweighted pair-group method with arithmetic averages (UPGMA) clearly differentiated  C. globosum  isolates from  C. perlucidum  and  C. reflexum . One of the primers, URP-2R,...
Palavras-chave: Chaetomium globosum; Chaetomium reflexum; Chaetomium perlucidum; Genetic variability; URP-PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500023

Genetic structure analysis of Eufriesea violacea (Hymenoptera, Apidae) populations from southern Brazilian Atlantic rainforest remnants
Provedor de dados: 74
Autores: Sofia,Silvia H.; Paula,Francine M. de; Santos,Aline M. dos; Almeida,Fernanda S.; Sodré,Leda M.K.
Random amplified polymorphic DNA (RAPD) markers were used to analyze the genetic structure of Eufriesea violacea populations in three fragments (85.47, 832.58 and 2800 ha) of Atlantic rainforest located in the north of the Brazilian state of Paraná. A total of twelve primers produced 206 loci, of which 129 were polymorphic (95% criterion). The proportions of polymorphic loci in each population ranged from 57.28% to 59.2%, revealing very similar levels of genetic variability in the groups of bees from each fragment. Unbiased genetic distances between groups ranged from 0.0171 to 0.0284, the smallest genetic distance occurring between bees from the two larger fragments. These results suggest that the E. violacea populations from the three fragments have...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Euglossine bees; Euglossina; Rainforest fragments; Genetic variability; RAPD.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300026

Allozyme variation among three populations of the armored catfish Hypostomus regani (Ihering, 1905) (Siluriformes, Loricariidae) from the Paraná and Paraguay river basins, Brazil
Provedor de dados: 74
Autores: Zawadzki,Cláudio H.; Renesto,Erasmo; Peres,Maria Dolores; Paiva,Suzana.
Three Brazilian populations of the armored catfish Hypostomus regani (Ihering, 1905) were sampled, one from the Corumbá Reservoir in Goiás state, another from the Itaipu Reservoir in Paraná state and a third from the Manso Reservoir in Mato Grosso state. Allozyme electrophoresis was used to establish the genetic structure of the species, with the analysis of liver, heart and muscles tissues allowing the scoring of 25 loci from 14 enzymatic systems. Although no diagnostic loci were found, some exclusive rare alleles were recorded for the three populations. The genetically most similar populations were those from Corumbá and Itaipu, and the most distant were the populations from Manso and Corumbá. The allozyme data showed three structured populations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; Genetic variability; Hypostomus regani; Loricariidae; Paraguay and Paraná Rivers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400025

Mitochondrial DNA variation in wild populations of Leporinus elongatus from the Paraná River basin
Provedor de dados: 74
Autores: Martins,Cesar; Wasko,Adriane P.; Oliveira,Claudio; Foresti,Fausto.
Leporinus elongatus, a fish species widely distributed throughout the Paraná River basin in South America, is an important fishery resource and a valuable species in aquaculture programs. Despite its great economic importance, several wild populations have been suffering a drastic reduction. The comprehension of its population structure represents an important step for the conservation of these organisms in natural environments, and also for the selection of wild stocks to be used in hatchery programs. In order to understand the genetic-population structure of L. elongatus, D-loop mitochondrial DNA analyses were applied in six wild populations of the species. The results were used to estimate the levels of within and among population genetic variability....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; D-loop; Genetic variability; Leporinus elongatus; MtDNA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100006

Esterase-3 polymorphism in the sugarcane borer Diatraea saccharalis (Lepidoptera, Pyralidae)
Provedor de dados: 74
Autores: Ruvolo-Takasusuki,Maria Claudia C.; Machado,Maria de Fátima P.S.; Conte,Hélio.
The migration rate of esterases and their substrate specificity for 4-methylumbelliferyl esters (acetate, propionate, and butyrate) and alpha- and beta-naphthyl esters were analyzed in Diatraea saccharalis by starch gel electrophoresis. Substrate preference of esterases was observed with Est-2 and Est-8 isozymes showing substrate specificity for 4-methylumbelliferyl esters and Est-4 isozyme showing specificity for 4-methylumbelliferyl butyrate and alpha-naphthyl butyrate. Allele variation was detected at the Est-3 locus. Two alleles, Est-3F and Est-3S, were identified in pupae with fluorogenic and ester-naphthyl substrates. Chi-square analysis showed no differences between the observed genotypic frequencies and those expected on the basis of Hardy-Weinberg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esterase; Genetic variability; Isozymes; Moth; Sugarcane borer.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100012

Genetic diversity of Xanthomonas axonopodis pv. citri based on plasmid profile and pulsed field gel electrophoresis
Provedor de dados: 74
Autores: Carvalho,Flávia Maria de Souza; Caramori,Lázara Pereira Campos; Leite Júnior,Rui Pereira.
Xanthomonas axonopodis pv. citri strains that cause disease in citrus were investigated by pulsed field and plasmid profile analysis. For the first method, genomic DNA was digested by the rare-cutting enzymes Xba I and Vsp I. The strains evaluated were collected in seven different States of Brazil and in Argentina, Bolivia, Paraguay and Uruguay. Genetic variability was found among strains of X. axonopodis pv. citri from different geographical areas Argentina, Bolivia and Uruguay, with similarities varying from 0.62 to 0.83. However, the strains collected in Brazil, despite being from different States, have shown a genetic similarity ranging from 0.83 to 1.00. Cluster analysis showed a relationship between genomic similarity and geographical origin of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Genetic variability; Bacterium; Citrus; PFGE.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300020

Genetic variability in Astyanax altiparanae Garutti &amp;amp; Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil
Provedor de dados: 74
Autores: Peres,Maria Dolores; Vasconcelos,Marcio dos Santos; Renesto,Erasmo.
Allozyme data was used to assess the genetic diversity Astyanax altiparanae populations from the floodplain of the Upper Paraná River (PR). Specimens were collected in the southern Brazilian state of Paraná from PR in Porto Rico municipality and Ribeirão Ficha (RF) in Ubiratã municipality. The authors used 15% (w/v) corn starch gel electrophoresis to identify 21 putative loci for 13 enzymatic systems: Aspartate aminotransferase, 2.6.1.1 (AAT), Acid phosphatase, 3.1.3.2 (ACP), Esterase, 3.1.1.1 (EST), Glycerol-3-phosphate dehydrogenase, 1.1.1.8 (G3PDH), Glucose-6-phosphate dehydrogenase, 1.1.1.49 (G6PDH), Glucose-6-phosphate isomerase, 5.3.1.9 (GPI), Iditol dehydrogenase, 1.1.1.14 (IDDH), Isocitrate dehydrogenase - NADP+, 1.1.1.42 (IDH), L-Lactate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; Astyanax; Fishes; Genetic variability; Paraná River floodplain.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500011

Genetic variability in geographical populations of Aedes aegypti (Diptera, Culicidae) in Brazil elucidated by molecular markers
Provedor de dados: 74
Autores: Paduan,Karina dos Santos; Araújo-Júnior,João P.; Ribolla,Paulo E.M.
The tropical mosquito, Aedes aegypti is the most important domestic vector of urban yellow fever and dengue. Genetic population studies on this vector are important because they may lead to new tools for surveillance. An analysis of genetic structure was conducted among populations of A. aegypti from 11 localities in four demographic regions within six Brazilian federal states. Markers included 21 random amplified polymorphic DNA (RAPD) loci. RAPD markers were detected among populations and cluster analysis revealed two main groups. We found high genetic polymorphism (H S = 0.224) and high levels of genetic differentiation between populations from different states (G ST = 0.430), as well as in populations from cities in the same state (G ST = 0.410). These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aedes aegypti; Population genetics; Random amplified polymorphic DNA; Genetic variability.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200030

Reproductive strategies and genetic variability in tropical freshwater fish
Provedor de dados: 74
Autores: Lassala,Maria Dolores Peres; Renesto,Erasmo.
We estimated the genetic variability of nine fish species from the Brazilian upper Paraná River floodplain (Astyanax altiparanae, Hoplias malabaricus, Leporinus lacustris, Loricariichthys platymetopon, Parauchenipterus galeatus, Pimelodus maculatus, Rhaphiodon vulpinus, Roeboides paranensis and Serrasalmus marginatus) based on data for 36 putative allozyme loci obtained using corn starch gel electrophoresis of 13 enzymatic systems: aspartate aminotransferase (EC 2.6.1.1), acid phosphatase (EC 3.1.3.2), esterase (EC 3.1.1.1), glycerol-3-phosphate dehydrogenase (EC 1.1.1.8), glucose-6-phosphate dehydrogenase (EC 1.1.1.49), glucose-6-phosphate isomerase (EC 5.3.1.9), Iditol dehydrogenase (EC 1.1.1.14), isocitrate dehydrogenase - NADP+ (EC 1.1.1.42), L-lactate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Heterozygosity; Paraná River; Polymorphism; Reproductive strategies; Tropical fish.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400030

Genetic characterization of Bhutanese native chickens based on an analysis of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), domestic Southeast Asian and commercial chicken lines (Gallus gallus domesticus)
Provedor de dados: 74
Autores: Dorji,Nedup; Duangjinda,Monchai; Phasuk,Yupin.
The genetic diversity of Bhutanese chickens needs to be understood in order to develop a suitable conservation strategy for these birds in Bhutan. In this, work, we used microsatellite markers to examine the genetic diversity of Bhutanese chickens. Four Bhutanese chicken varieties (Black plumage, Frizzle, Naked neck and Red Junglefowllike, corresponding to Yuebjha Narp, Phulom, Khuilay and Seim, respectively), two subspecies of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), two varieties of Thai native chickens (Pradhu Hang Dam and Chee; Gallus gallus domesticus) representing the Southeast Asian domestic chicken, and two commercial lines (Broiler and Single Comb White Leghorn) were genotyped with 18 microsatellites that included 16...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Genetic comparisons; Genetic variability; Microsatellites.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400010

Genetic variability of Herpailurus yagouaroundi, Puma concolor and Panthera onca (Mammalia, Felidae) studied using Felis catus microsatellites
Provedor de dados: 74
Autores: Moreno,Vanessa Roma; Grisolia,Alexéia Barufatti; Campagnari,Francine; Milazzotto,Marcela; Adania,Cristina Harumi; Garcia,José Fernando; Souza,Edislane Barreiros de.
We used four microsatellite loci (Fca08, Fca45, Fca77 and Fca96) from the domestic cat, Felis catus, to investigate genetic variability in specimens of Herpailurus yagouaroundi (jaguarundi, otter cat, eyra), Puma concolor (cougar, mountain lion, puma) and Panthera onca (jaguar) held in various Brazilian zoos. Samples of DNA from the cats were PCR amplified and then sequenced before being analyzed using the CERVUS program. Our results show a mean polymorphic information content (PIC) of 0.83 for H. yagouaroundi, 0.66 for P. concolor and 0.69 for P. onca and a mean of 10.3 alleles for the Fca08 locus, 5.3 for Fca 45, 9 for Fca 77 and 14 for Fca 96. These results indicate a relatively high level of genetic diversity for the specimens studied.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Mammals; Felines; Microsatellites.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200017

Genetic polymorphisms and haplotypes of the organic cation transporter 1 gene (SLC22A1) in the Xhosa population of South Africa
Provedor de dados: 74
Autores: Jacobs,Clifford; Pearce,Brendon; Du Plessis,Mornè; Hoosain,Nisreen; Benjeddou,Mongi.
Human organic cation transporter 1 is primarily expressed in hepatocytes and mediates the electrogenic transport of various endogenous and exogenous compounds, including clinically important drugs. Genetic polymorphisms in the gene coding for human organic cation transporter 1, SLC22A1, are increasingly being recognized as a possible mechanism explaining the variable response to clinical drugs, which are substrates for this transporter. The genotypic and allelic distributions of 19 nonsynonymous and one intronic SLC22A1 single nucleotide polymorphisms were determined in 148 healthy Xhosa participants from South Africa, using a SNAPshot® multiplex assay. In addition, haplotype structure for SLC22A1 was inferred from the genotypic data. The minor allele...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Haplotype; Population genetic structure; Genotyping; Genetic variability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300006

Genetic diversity and DNA content of three South American and three Eurasiatic Trifolium species
Provedor de dados: 74
Autores: Rizza,Marco Dalla; Real,Daniel; Reyno,Rafael; Porro,Valentina; Burgueño,Juan; Errico,Eugenia; Quesenberry,Kenneth H.
Six species of Trifolium (T. polymorphum Poir., T. riograndense Burkart, T. argentinense Speg., T. medium L., T. pratense L. and T. repens L.) were analyzed using inter-simple sequence repeats (ISSR) markers. Six selected primers generated 186 polymerase chain reaction (PCR) products exploring 112 loci in 34 genotypes analyzed with molecular sizes ranging from 200 to 1300 bp. These primers were able to discriminate among and within species, with the PCR products being on average 41.6% species-specific and 59.9% polymorphic at the within species level. Nuclear DNA content was determined by flow cytometry and revealed variation among species. The 1Cx genome size values were calculated and were found to range from 0.46 pg (T. pratense) to 0.96 pg (T....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA content; Genetic variability; Inter-SSR; Trifolium spp.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600015

Allozyme differentiation of two populations of the genus Neoplecostomus Eigenmann &amp;amp; Eigenmann, 1888 (Teleostei, Loricariidae) from the upper Paraná River basin, Brazil
Provedor de dados: 74
Autores: Reusing,Ana Flávia; Renesto,Erasmo; Roxo,Fábio F.; Zawadzki,Cláudio H.
Allozyme electrophoresis was used to examine 12 enzymatic systems in two populations of the genus Neoplecostomus from the Paraná River basin. Samples of Neoplecostomus sp. 1 were collected in Paraitinguinha stream of the Tietê River basin, in the municipality of Salesópolis, São Paulo State, and those of Neoplecostomus sp. 2 from São Domingos stream of the Rio Grande River basin, in the municipality of Muzambinho, Minas Gerais State. The genetic variability of the two populations was estimated by Nei's expected heterozygosity and was considered lower than average for populations of freshwater fish. The proportion of polymorphic loci was low (only 5.26% for the locus Idh). The low frequency of heterozygosity for both populations revealed a high fixation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; Genetic variability; Neoplecostominae; Neotropical fishes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300021

Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
Provedor de dados: 74
Autores: Kumar,Dinesh; Gupta,Neelam; Ahlawat,S.P.S.; Satyanarayana,R.; Sunder,Shyam; Gupta,S.C.
The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect &lt;FONT FACE=Symbol&gt;a&lt;/font&gt;-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the a-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These a-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-lactalbumin; Genetic variability; Goat; Single-strand conformation polymorphism (SSCP).
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200016

Microsatellite variability analysis in farmed catfish (Ictalurus punctatus) from Tamaulipas, Mexico
Provedor de dados: 74
Autores: Perales-Flores,Laura E.; Sifuentes-Rincón,Ana María; León,Francisco J. García de.
Analysis of cultured catfish from six farms in Tamaulipas, Mexico was achieved using a combination of microsatellite PCR analysis and semiautomatic fluoresce-based detection, in order to provide a first assessment of the genetic variability on cultured catfish in Mexico. Five microsatellites showed extensive polymorphism with allele numbers ranging from 10 and 20. Overall observed heterozygosity at each locus ranged between 0.76 and 0.91 and the average polymorphic information content (PIC) for the five loci was 0.811, indicating that these loci can be used for studies of paternity identification, linkage and population genetics. On the basis of the F ST values (F ST = 0.03829; p = 0.00000) it appears that there was a small amount of genetic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ictaluridae; Molecular characterization; Genetic variability.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400011

Genetic diversity among maize (Zea mays L.) landraces assessed by RAPD markers
Provedor de dados: 74
Autores: Carvalho,Valdemar P.; Ruas,Claudete F.; Ferreira,Josué M.; Moreira,Rosângela M.P.; Ruas,Paulo M.
The genetic relationships among 81 maize accessions consisting 79 landraces and two improved varieties, maintained by farmers in southern Brazil were investigated using Random Amplified Polymorphic DNA (RAPD). Thirty-two highly informative primers amplified 255 markers of which 184 (72.2%) were polymorphics. Based on the RAPD markers, a dendrogram was constructed using the UPGMA method. The range of genetic similarity was from 0.78 to 0.91. The molecular data grouped the accessions into two main clusters, which were correlated according to kernel colors. Small clusters were seen associated to characteristics, such as kernel morphology. The analysis of the molecular data revealed that maize management adopted by small-scale farmers has contributed to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn landrace; Genetic variability; Molecular genetic markers; RAPD.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200017

Population structure analysis of Pimelodus maculatus (Pisces, Siluriformes) from the Tietê and Paranapanema Rivers (Brazil)
Provedor de dados: 74
Autores: Almeida,Fernanda Simões de; Sodré,Leda Maria Koelblinger; Contel,Eucleia Primo Betioli.
Pimelodus maculatus populations from the Tietê and Paranapanema rivers were sampled and had their genetic structure analyzed by using RAPD markers, with the aim of contributing to future conservation studies. The proportion of polymorphic loci was greater than 50% in the populations of both rivers. Genetic diversity data showed that, in spite of its nine hydroelectric plants, the Tietê river population was genetically homogeneous, whereas the Paranapanema river population was structured. This might be due to the presence of high waterfalls distributed all along its course. These data may serve as indicators for future conservation studies on the Tietê and Paranapanema rivers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD markers; Population structure; Conservation; Genetic variability; Pimelodus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300014

Genetic diversity in cultivated carioca common beans based on molecular marker analysis
Provedor de dados: 74
Autores: Perseguini,Juliana Morini Küpper Cardoso; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Colombo,Carlos Augusto; Carbonell,Sérgio Augusto Moraes; Mondego,Jorge Mauricio Costa; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Campos,Tatiana de; Souza,Anete Pereira de; Rubiano,Luciana Benchimol.
A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLPs; Genetic structure; Genetic variability; Phaseolus vulgaris L.; SSRs.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017

Genetic relationships among American species of Prosopis (Leguminosae) based on enzyme markers
Provedor de dados: 74
Autores: Bessega,Cecilia; Saidman,Beatriz Ofelia; Vilardi,Juan César.
In the present work, isoenzyme electrophoresis was used to analyze the variability and phenetic relationships among seven American species of genus Prosopis belonging to three different sections: P. argentina (Monilicarpa), P. glandulosa, P. velutina, P. flexuosa, P. ruscifolia, P. kuntzei (Algarobia), and P. reptans (Strombocarpa). The genetic variability in P. argentina, P. reptans, and P. kuntzei was significantly lower than in the rest of the species analyzed. The species belonging to different sections are highly differentiated, but the relationships retrieved among species belonging to the section Algarobia suggested that the series of this section are not natural groups. P. kuntzei is as differentiated from the remaining species of Algarobia as from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prosopis; Isoenzymes; Genetic variability; Phenetic relationships.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200018

Environmental interaction, additive and non-additive genetic variability is involved in the expression of tissue and whole-plant heat tolerance in upland cotton (Gossypium hirsutum. L)
Provedor de dados: 74
Autores: Hafeez-ur-Rahman,.
Heat tolerance is measured at tissue level by cellular membrane thermostability (CMT) and at the whole plant level by the heat tolerance index (HTI). Eight upland cotton cultivars and 15 crosses were used to determine the type and extent of genetic variability associated with the expression of these traits between and within environments. Heat stress and non-stress conditions were used as the CMT environments and years for HTI. The wide variation in heterotic expression and combining ability effects observed for CMT and HTI suggest multigenic inheritance of these traits. Significant genetic variability across environments was evident but the traits were not highly heritable because of substantial environmental interaction. The available genetic variability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotton; Environmental interaction; Genetic variability; Heat tolerance; Membrane stability.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300022

Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers
Provedor de dados: 74
Autores: Silva,Roxane Wirschum; Freitas,Thales R.O. de; Sbalqueiro,Ives José.
In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the 16 alleles in collared peccaries, and 3 of the 10 alleles in the white-lipped peccaries. Polymorphic information content (PIC) in both species and all the loci was highly informative. The probability of paternity exclusion (PEC), if one of the parents is known, was almost as high in white-lipped peccaries (95.53%) as in the collared (99,48%). The Fst values for collared (0.042) and white-lipped...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite markers; Tayassu pecari; Pecari tajacu; Genetic variability; Animals of captivity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100012

High genetic diversity and connectivity in Colossoma macropomum in the Amazon basin revealed by microsatellite markers
Provedor de dados: 74
Autores: Fazzi-Gomes,Paola; Guerreiro,Sávio; Palheta,Glauber David Almeida; Melo,Nuno Filipe Alves Correa de; Santos,Sidney; Hamoy,Igor.
Abstract Colossoma macropomum is the second largest scaled fish of the Amazon. It is economically important for commercial fisheries and for aquaculture, but few studies have examined the diversity and genetic structure of natural populations of this species. The aim of this study was to investigate the levels of genetic variability and connectivity that exist between three natural populations of C. macropomum from the Amazon basin. In total, 247 samples were collected from the municipalities of Tefé, Manaus, and Santarém. The populations were genotyped using a panel of 12 multiplex microsatellite markers. The genetic diversity found in these populations was high and similar to other populations described in the literature. These populations showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tambaqui; Genetic variability; Gene flow; Genetic structure; Single sequence repeats.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100142

Populational genetic structure and sociogenetic structure of cocoon masses of Digelasinus diversipes (Kirby, 1882) (Hymenoptera: Symphyta: Argidae)
Provedor de dados: 74
Autores: Boraschi,Daniele; Del Lama,Marco Antonio.
Gene variation and population genetic structure of the Neotropical sawfly Digelasinus diversipes were measured by allozyme analyses using starch gel electrophoresis. Cocoon masses were collected in Eugenia glazioviana (Myrtaceae) stems, in two areas of the "Estação Ecológica Jataí" (Luiz Antônio, SP, Brazil - 21°25' S, 47°50' W), in 2000 and 2001. The average heterozygosity observed in this species (Hobs = 0.094 ± 0.025) was not significantly different from other Symphyta groups; it was, however, significantly higher than in other Hymenoptera populations. No significant levels of inbreeding were found (F IS = 0.062; chi2 = 29.9; p &gt; 0.05), but the population was subdivided (F ST = 0.070; chi2 = 458.9; p &lt; 0.05), suggesting the absence of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Population genetics; Digelasinus diversipes; Neotropical sawfly; Allozymes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300013

Population structures of Brazilian Tall coconut (Cocos nucifera L.) by microsatellite markers
Provedor de dados: 74
Autores: Ribeiro,Francisco E.; Baudouin,Luc; Lebrun,Patricia; Chaves,Lázaro J.; Brondani,Claudio; Zucchi,Maria I.; Vencovsky,Roland.
Coconut palms of the Tall group were introduced to Brazil from the Cape Verde Islands in 1553. The present study sought to evaluate the genetic diversity among and within Brazilian Tall coconut populations. Samples were collected of 195 trees from 10 populations. Genetic diversity was accessed by investigating 13 simple sequence repeats (SSR) loci. This provided a total of 68 alleles, ranging from 2 to 13 alleles per locus, with an average of 5.23. The mean values of gene diversity (He) and observed heterozygosity (Ho) were 0.459 and 0.443, respectively. The genetic differentiation among populations was estimated at θ = 0.1600 and the estimated apparent outcrossing rate was t a = 0.92. Estimates of genetic distances between the populations varied from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coconut; Genetic diversity; Genetic variability; Molecular markers; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400016

Very low mitochondrial variability in a stingless bee endemic to cerrado
Provedor de dados: 74
Autores: Brito,Rute Magalhães; Francisco,Flávio de Oliveira; Françoso,Elaine; Santiago,Leandro Rodrigues; Arias,Maria Cristina.
Partamona mulata is a stingless bee species endemic to cerrado, a severely threatened phytogeographical domain. Clearing for pasture without proper soil treatment in the cerrado facilitates the proliferation of termite ground nests, which are the nesting sites for P. mulata. The genetic consequences of these changes in the cerrado environment for bee populations are still understudied. In this work, we analyzed the genetic diversity of 48 colonies of P. mulata collected throughout the species' distribution range by sequencing two mitochondrial genes, cytochrome oxidase I and cytochrome B. A very low polymorphism rate was observed when compared to another Partamona species from the Atlantic forest. Exclusive haplotypes were observed in two of the five areas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; MtDNA; Genetic variability; Partamona mulata.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100018

Genetic divergence among eggplant genotypes under high temperatures
Provedor de dados: 75
Autores: Valadares,Ricardo de N; Nóbrega,Danieli A; Lima,Lilian B de; Mendes,Adônis Q; Silva,Fabian S; Melo,Roberto de A; Menezes,Dimas.
ABSTRACT The aim of this study was to estimate the genetic divergence among eggplant genotypes for agronomic traits in order to gather information for the selection of genotypes in eggplant breeding programs for tolerance to high temperatures. Ten traits recommended by the International Board for Plant Genetic Resources were analyzed in 24 genotypes, arranged in a randomized complete block design with four replicates. Data were submitted to analysis of variance (P&lt;0.01) and later to the UPGMA and Tocher grouping methods, using the generalized Mahalanobis distance (D2) as dissimilarity measure. Three and six groups of similarity were obtained, respectively, for the multivariate techniques used, UPGMA and Tocher, with concordance in the grouping of 87.50%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum melongena; Genetic variability; Protected cultivation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000300272

Multivariate techniques in the determination of genetic diversity in pest-resistant mini tomato genotypes
Provedor de dados: 75
Autores: Maciel,Gabriel M; Peixoto,Joicy Vitória M; Rocha,Jaíne Priscila R; Almeida,Rafaela S; Oliveira,Camila S; Mendonça,Thiago Fellipe N.
ABSTRACT The objective of this study was to compare methods of multivariate analysis on the evaluation of genetic diversity of mini tomato and to identify promising genotypes with resistance to pests. The experiment was conducted at the Vegetable Experiment Station of the Universidade Federal de Uberlândia, Monte Carmelo campus, from April 2013 to November 2016. The experimental design was a randomized complete block design with 16 treatments and four replications totaling 64 plots, and each plot represented by five plants. Sixteen genotypes were characterized, 12 from the F2RC1 generation, obtained through the interspecific crossing between the wild access LA-716 (Solanum pennellii) and pre-commercial lines of mini tomato (UFU-73 and UFU-2) (Solanum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum pennellii; Solanum lycopersicum; Acyl sugar; Genetic variability.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400504

Biochemical descriptors: importance of the genetic divergence study in peppers
Provedor de dados: 75
Autores: Araújo,Lourismar M; Neves,Leonarda G; Sousa,Demétrio A; Zeviani,Walmes M; Silva,Lucinéia da R; Marostega,Thalita N.
ABSTRACT The genetic diversity of pepper genotypes is usually evaluated based on morphological and molecular descriptors. However, studies using biochemical descriptors of peppers are still incipient. Thus, the objective of this study was identify biochemical descriptors of Capsicum spp. with antioxidant action, most responsive to the selection of superior genotypes, and evaluate the correlation between concentrations of the parameters using principal components analysis. The experiment was conducted in the city of Cáceres, southwest Mato Grosso, Brazil. Seventy-eight pepper accessions from the Active Germplasm Bank of UNEMAT were planted in randomized block design. The harvested fruits were immersed in liquid nitrogen for two minutes, and the following...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum spp.; Main components; Genetic variability.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000200210

Multicategorical descriptors for creole genotypes of Amazon chicory (Eryngium foetidum)
Provedor de dados: 75
Autores: Gomes,Rafaelle F; Gonçalves,Viviane P; Arruda,Renata da S; Santos,Lucas da S.
ABSTRACT The knowledge concerning genetic variability of creole genotypes by using morphological descriptors in species such as Amazon chicory is crucial to quantify local diversity and protect improved cultivars. Accordingly, this study aimed to propose multicategory leaf morphological descriptors for Amazon chicory species. To achieve that, an experiment was arranged in randomized block design with four repetitions. The treatments comprised eight creole genotypes of Amazon chicory, which were obtained from local producers’ collection. The proposed multi-categorical descriptors are based on limb shape, leaf apex shape, leaf base shape, leaf margin, bract consistency, and bract margin traits. Once the multicategorical clustered data were obtained, they...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pará chicory; Landraces; Genetic variability.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000300334

Genetic diversity in accessions of melon belonging to momordica group
Provedor de dados: 75
Autores: Valadares,Ricardo N; Melo,Roberto A; Sarinho,Isabel VF; Oliveira,Natália S; Rocha,Fernando AT; Silva,José W; Menezes,Dimas.
ABSTRACT The genetic divergence of melon genotypes belonging to momordica group, collected in five Brazilian States, was estimated, and the relative contribution of the morphological characters was determined for the genetic variability. The experimental design was randomized blocks, with four replicates. We evaluated 19 accessions of melon, momordica group, two accessions of cantaloupensis group and two commercial cultivars of inodorus group. These genotypes were characterized by 42 morphological descriptors. The data were submitted to Tocher and UPGMA grouping methods using the genetic dissimilarity matrix, using Mahalanobis’ distance. Singh criterion was used to identify the relative contribution of each character to the genetic divergence. Four groups...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cucumis melo var. momordica; Genetic variability; Snow melon; Papoco melon.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000200253

Prediction of genetic gains through selection of sweet potato accessions
Provedor de dados: 75
Autores: Vargas,Pablo F; Otoboni,Maria Eduarda F; Lopes,Beatriz G; Pavan,Bruno E.
ABSTRACT Due to the high genetic variability found in sweet potato and a low number of cultivars available on the market, there are opportunities for necessary improvements in crop breeding programs. The selection indexes are a favorable strategy to achieve higher yields through genetic gains obtained with the future population. Thus, the objective was to evaluate the selection gain of agronomic characters from sweet potato accessions for root production and dual-aptitude. 95 accessions and two commercial cultivars (Braslandia Branca and Brazlândia Roxa) were evaluated. A randomized block design with three replications of ten plants per plot was used. The index proposed by Mulamba &amp; Mock was used to select superior individuals. The evaluated population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ipomoea batatas; Plant breeding; Genetic variability.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000400387

Agronomic potential and selection of okra hybrids to obtain potential genitors
Provedor de dados: 75
Autores: Maciel,Gabriel M; Finzi,Rafael R; Marra,Alexandre William C; Carvalho,Fábio J; Nogueira,Ana Paula O.
ABSTRACT Evaluation of pre-commercial hybrids in a germplasm bank is essential for determining its commercial potential or its utility as a potential genitor in a breeding program. The objective of this study was to determine genetic divergence and per se behavior of 47 pre-commercial hybrids from okra germplasm bank of the Universidade Federal de Uberlândia. Precocity index (%), number of fruits (fruits per plant), average fruit mass (g) and productivity (g per plant) were evaluated. Analysis of genetic divergence was performed by multivariate analysis using Mahalanobis distance with different clustering methods (UPGMA and canonical analysis). The performance of hybrids was compared by Scott-Knott (p= 0.05). A significant genetic variability among okra...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abelmoschus esculentus; Genetic variability; Heterosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000100112

Analysis of phenotypes altered by temperature stress and hipermutability in Drosophila willistoni
Provedor de dados: 76
Autores: D'Ávila,Marícia F.; Garcia,Rosane N.; Loreto,Elgion L. S.; Valente,Vera Lúcia da S.
Drosophila willistoni (Sturtevant, 1916) is a species of the willistoni group of Drosophila having wide distribution from the South of USA (Florida) and Mexico to the North of Argentina. It has been subject of many evolutionary studies within the group, due to its considerable ability to successfully occupy a wide range of environments and also because of its great genetic variability expressed by different markers. The D. willistoni 17A2 strain was collected in 1991 in the state of Rio Grande do Sul, Brazil (30°05'S, 51°39'W), and has been maintained since then at the Drosophila laboratory of UFRGS. Different to the other D. willistoni strains maintained in the laboratory, the 17A2 strain spontaneously produced mutant males white-like (white eyes) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: D. willistoni; 17A2 strain; Temperature stress; Genetic variability; Hypermutability.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212008000300009

Effect of age and environment on the summer mortality in cupped oyster Crassostrea gigas during the first two years
Provedor de dados: 5
Autores: Degremont, Lionel; Boudry, Pierre; Soletchnik, Patrick; Bedier, Edouard; Ropert, Michel; Samain, Jean-francois.
Three successive generations were produced between 2001 and 2003 to assess to what extent genetic variability exists for this trait in juvenile oysters Crassostrea gigas.  For each generation, two groups were selected for their high ("R" for resistant) and low ("S" for susceptible) survival.  Significant differences of mortality were observed during the first year in Rivière d'Auray (RA) (Brittany-France).  However, low and similar mortality were observed for both groups, with no significant difference between groups in the first and second generation in this site, where the first summer is the critical sensitive period with a clear age effect.  In Baie des Veys (BDV) (Normandie-France), where mortalities usually mainly affect adults, mortality between...
Tipo: Text
Palavras-chave: Genetic variability; Genetic; Summer mortality; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3442.pdf

Overwiew on selective breeding and genetic improvement in bivalve shellfish
Provedor de dados: 5
Autores: Boudry, Pierre.
Dr. Boudry focussed his talk on oysters, which are the most important bivalve species produced world wide (more than 4 million tons). One of the main characteristic of bivalve aquaculture is that most of the juveniles ("seed or "spat") are collected from natural recruitment (e.g. 100% of the European mussel production). Hatchery propagation is used in the case of introduced species (e.g. Crassostrea gigas in USA)  or limited natural recruitment.    The most significant genetic improvement for the production of Pacific oyster (C. gigas) to date has been obtained through the breeding of triploids, especially since the development of tetraploids. Triploidy lead to highly reduced gametogenesis and, as a result better growth and survival.  Many quantitative...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oyster; Genetic variability; Bivalve shellfish; Selective breeding; Genetic.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3479.pdf

Assessment of Genetic Variability of Fish Personality Traits using Rainbow Trout Isogenic Lines
Provedor de dados: 5
Autores: Millot, Sandie; Pean, Samuel; Labbe, Laurent; Kerneis, Thierry; Quillet, Edwige; Dupont-nivet, Mathilde; Begout, Marie-laure.
The study of inter-individual variability of personality in fish is a growing field of interest but the genetic basis of this complex trait is still poorly investigated due to the difficulty in controlling fish genetic origin and life history. When available, isogenic lines that allow performing independent tests on different individuals having identical genotype constitute a very relevant experimental material to disentangle the genetic and environmental components of behavioural individuality. We took advantage of heterozygous isogenic lines to investigate the personality in rainbow trout through the analysis of their reactions to different experimental situations. To this end, seven to ten rainbow trout isogenic lines were screened for their spatial...
Tipo: Text
Palavras-chave: Personality; Isogenic lines; Genetic variability; Risk taking; Spatial exploration; Oncorhynchus mykiss.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00194/30496/29976.pdf

Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
The most significant genetic improvement for production of Pacific oyster (Crassastrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have slowed the development of selective breeding programs. Recently, in the USA, Australia and New Zealand, family-based selective breeding programs have been initiated to improve growth and yield. In Europe, where both natural and...
Tipo: Text
Palavras-chave: Microsatellites; Quantitative genetic; Genetic variability; Crassostrea gigas; Oysters.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2861.pdf

Is avoiding full sib matings really worthwhile in a breeding program?
Provedor de dados: 5
Autores: Dupont-nivet, Mathilde; Vandeputte, Marc.
Stochastic simulations were used to compare genetic progress and inbreeding depending on whether full sib matings were allowed or not when applying individual selection. Fifteen selection cycles were considered, using a single pair mating design. Two levels of heritability (h(2)) were studied (0.1; 0.5) as well as four different proportions of selected animals (2, 5, 10 and 20%), four population sizes (1000, 2,000, 4,000 and 10,000 candidates) and two levels of inbreeding depression (0 and 5% decrease of phenotype for 10% increase in inbreeding). Fifty replicates were generated for each set of parameters. The simulations revealed that avoiding full sib matings became less and less relevant as population size and proportion of selected animals increased and...
Tipo: Text
Palavras-chave: Selection; Sib mating; Inbreeding; Genetic variability; Stochastic simulations; Fish.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00035/14587/11952.pdf

Residual genetic variability in domesticated populations of the Pacific blue shrimp (Litopenaeus stylirostris) of New Caledonia, French Polynesia and Hawaii and some management recommendations
Provedor de dados: 5
Autores: Goyard, Emmanuel; Arnaud, Sophie; Vonau, Vincent; Bishoff, Vincent; Mouchel, Olivier; Pham, Dominique; Wyban, Jim; Boudry, Pierre.
The Latin American shrimp Litopenaeus stylirostris was introduced in three different Pacific islands (Tahiti, New Caledonia via Tahiti, and Hawaii) and hatchery-propagated for 7-25 generations to develop shrimp farming based on these domesticated stocks. Three microsatellite markers have been used in an attempt to assess the genetic bases of the populations available to start a selective breeding program. The comparison of eight hatchery stocks (five New Caledonian, two Hawaiian and one Tahitian stocks) and one wild Ecuadorian population showed a much lower variability in the domesticated stocks than in the wild population, especially in New Caledonia and Tahiti (2-3.7 vs. 14-27 alleles per locus; 20-60% vs. 90% expected heterozygosity). The Tahitian and...
Tipo: Text
Palavras-chave: Microsatellite marker; Genetic variability; Domestication; Litopenaeus stylirostris; Shrimp.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-596.pdf

Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas) (slides)
Provedor de dados: 5
Autores: Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained in diploids using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. Recently, in the U.S.A., Australia and New Zealand, countries where hatcheries are a major source of C. gigas juveniles, family-based selective...
Tipo: Text
Palavras-chave: Microsatellite markers; Selective breeding; Genetic; Genetic variability; Crassostrea gigas; Pacific oysters.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3449.pdf

Response to unintentional selection for faster development associated with inbreeding depression in Crassostrea gigas larvae, resulting from a broodstock selective breeding plan
Provedor de dados: 5
Autores: Taris, Nicolas; Batista, Frederico; Marissal, Eric; Boudry, Pierre.
Direct and indirect consequences of selective breeding in marine bivalves still remain largely unexplored. For species with two-phase life cycle, like the Pacific oyster (Crassostrea gigas), most studies have focused on juvenile and adult stages, but relatively few have focused on juvenile and adult stages, but relatively few have considered the larval stage, especially in a domestication context. We assessed the impact of hatchery practices on larval traits, notably on larval growth (due to the culling of slow growing larvae), by the study of larval progenies. Larvae originating from crosses using parental oysters both from natural beds and from hatchery broodstock which had been selected for adult growth and shell shape for seven generations. A set of...
Tipo: Text
Palavras-chave: Inbreeding depresion; Larvae stage; Genetic variability; Hatchery; Domestication; Genetic; Crassostrea gigas; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3337.pdf

Impact of intensive larval rearing practices in oyster hatcheries : selective processes and loss of genetic variability (Poster)
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Early-life history of oysters, like in most marine bivalves, is characterized by high fecundity and low survival rate. In hatcheries, which are ecoming more and more important for the production of juveniles for aquaculture production, rearing conditions are optimised (density, feeding, temperature...), and contrast with those observed in the wild. Additionally, slow growing larvae are culled by sieving.
Tipo: Text
Palavras-chave: Crassostrea gigas; Juvenile; Genetic variability; Selection; Genetic; Hatcheries; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3444.pdf

Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Boudry, Pierre.
Several studies have suggested that significant genetic variability might exist for some early developmental traits in the Pacific oyster, C. gigas. However, most studies have examined only limited numbers of families.  More critically, these families were rarely replicated, leading to possible bias due to heterogeneous larval rearing conditions among families. To overcome these potential problems, we used a mixed-family approach with subsequent parentage analysis. A recently developed set of three multiplexed microsatellite markers was used to determine parentage in a complete factorial cross between 12 males and 4 females. Larvae were reared at two different temperatures (20 and 26°C) to provide a contrast resembling wild versus hatchery conditions....
Tipo: Text
Palavras-chave: Selection; Genetic variability; Microsatellites; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3440.pdf

Genetic effect of domestication selective pressures on Pacific oyster at larval stage
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Boudry, Pierre.
Among bivalve species, the Pacific oyster, Crassostrea gigas, is the most economically important bivalve production over the world. Today, C. gigas is subject to an important production effort that leads to an intensive artificial selection. Larval stage is relatively unknown, specifically in a domestication context. Genetic consequence of artificial selection is still at a preliminary study. We aimed to tackle the consequence of inconscient domestication on the variance reproductive success focusing on larval stage, keystone of the life cycle. We studied two kinds of specific selective processes that common hatchery rearing practices exert : the effect of discarding the smallest larvae on genetic diversity and the artificial environment rearing effect via...
Tipo: Text
Palavras-chave: Selection; Genetic correlations; Genetic variability; Genetic; Domestication selective; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3441.pdf

Genetic differentiation in Gammarus fossarum and G. caparti (Crustacea, Amphipoda) with reference to G. pulex pulex in northwestern Europe
Provedor de dados: 16
Autores: Scheepmaker, Maarten; Dalfsen, Jan van.
Genetic differentiation among G. fossarum Koch, 1835 from different stations in Germany, Switzerland, Belgium and northern France, and the closely related Belgian form G. caparti Pètre-Stroobants, 1980 was investigated by electrophoresis at 20 enzyme loci. Although morphologically variable, geographically distant populations of G. cf. fossarum were hitherto considered conspecific. In the present study, populations of G. cf. fossarum and G. caparti were examined with reference to G. pulex pulex as an estimate for genetic differentiation at the species level. With G. p. pulex as a standard, genetic differentiation among geographically distant populations of G. cf. fossarum is occasionally observed at species level. The populations of G. caparti studied were...
Tipo: Article / Letter to the editor
Palavras-chave: Taxonomy; Distribution; Morphology; Genetic variability; Gammarus.
Ano: 1989
URL: http://www.repository.naturalis.nl/record/504137

Isoenzymatic polymorphism in the leaf-cutting ant Atta capiguara Gonçalves (hymenoptera: formicidae)
Provedor de dados: 82
Autores: Cantagalli,Liriana B; Mangolin,Claudete A; Ruvolo-Takasusuki,Maria C C.
This study was carried out to analyze the genetic population structure of Atta capiguara from 12 nests collected in Tapejara in the state of Paraná, Brazil, using isoenzyme polymorphisms. The analyzed isoenzymes were esterases (EST - EC 3.1.1.1), acid phosphatase (ACP - EC 3.1.3.2) and carbonic anhydrase (CA - EC 4.2.1.1). Ten loci were found in A.capiguara and four polymorphic loci were detected. The observed heterozigosity (0.0296) was low when compared to the expected heterozigosity (0.1461). The high value of F IS (0.7954) shows an excess of homozygous genotypes probably caused by inbreeding.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoenzyme; Genetic variability; Population genetics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000100007

RAPD variation within and among species of Melittobia Westwood (Hymenoptera: Eulophidae)
Provedor de dados: 82
Autores: Sari,Eloisa H.R.; Tavares,Mara G.; Campos,Lucio A.O.
The genus Melittobia Westwood comprises several species of microparasitoids and only two of them are know to occur in Brazil up to now: M. australica Girault and M. hawaiiensis Perkins. Nevertheless, the differentiation between these two species using traditional taxonomy is very difficult. In the present study, we used random amplified polymorphic DNA chain reaction (RAPD-PCR) to test for its ability to discriminate between these two species and to examine the genetic variation among the studied populations of M. australica. Most of the generated fragments were species-specific, occurring in all individuals of one species and absent in the individuals of the other species demonstrating the appropriateness of such technique to distinguish between both of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melittobia australica; M. hawaiiensis; Molecular marker; Genetic variability; Microparasitoid.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000200007

Genetic similarity among Agistemus pallinii Matioli et al (Acari: Stigmaeidae) found in citrus orchards in Viçosa, Minas Gerais State, Brazil
Provedor de dados: 82
Autores: Matioli,André L; Pallini,Angelo; Tavares,Mara G.
Stigmaeidae are very important predators of mite and insect pests on several crops in Brazil. It is considered the second most important family of predatory mites in citrus orchards in Brazil. However, their identification, especially that of the members of the genus Agistemus, is rather difficult based only on morphology. Hence, this study describes the use of random amplified polymorphic DNA (RAPD-PCR) markers to determine the genetic similarity of an Agistemus pallinii Matioli et al population found in 2004 in a citrus orchard in Viçosa, Minas Gerais State, Brazil, preying on Panonychus citri (McGregor). Amplifi cations were performed with 12 random primers (OPAA8, OPAA19, OPAB1, OPAB5, OPAB18, OPAC9, OPAC17, OPAC19, OPAD10, OPAE9, OPAE12 and OPAE17),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Predatory mite; Genetic variability; Molecular marker.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000200015

Estrutura genética populacional de Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) utilizando marcadores microssatélites
Provedor de dados: 82
Autores: Valle,GE do; Zucchi,MI; Stabellini,NS; Lourenção,AL; Pinheiro,JB.
We aimed to characterize the population genetic structure within and among five Bemisia tabaci (Gennadius) populations collected from different host plants and geographic regions by using microssatelites as a molecular marker. Each population was represented by 19 specimens. The host plants and geographic origins of these populations were described as follows: Pop 1: Squash Barreiras (BA); Pop 2: Cotton Barreiras (BA); Pop 3: Soybean Campinas (SP); Pop 4: Tomato Cruz das Almas (BA); and Pop 5: Soybean Rondonópolis (MT). Six polymorphic loci were observed, which discriminated 31 different alleles in the studied populations, with a mean number of alleles per population of 3.30 (2.67 - 4.00). Using Fisher's Exact test, it was observed that at least three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Molecular markers; Whitefly.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000200008

Mitochondrial DNA divergence between wild and laboratory populations of Anopheles albimanus Wiedemann (Diptera: Culicidae)
Provedor de dados: 82
Autores: Arias,Lida; Bejarano,Eduar E.; Márquez,Edna; Moncada,John; Vélez,Iván; Uribe,Sandra.
Studies of insect vectors may be facilitated by using laboratory colonies. However, it has been suggested that the colony insects are not representative of natural populations, sometimes yealding to erroneous interpretations of the intraspecific genetic variation between the individuals. In the present study the variability of the mitochondrial gene cytochrome b was evaluated among a closed laboratory colony of Anopheles albimanus that was founded 20 years ago and the field population from which it was derived. The analyses revealed the presence of five and three nucleotide haplotypes in the wild and colony populations, respectively. Wild individuals presented greater variability than those of the colony based on the number of polymorphic sites, haplotype...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Malaria; Mosquito.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300020

Characterization and genetic relationships among Brazilian biotypes of Schizaphis graminum (Rondani) (Hemiptera: Aphididae) using RAPD markers
Provedor de dados: 82
Autores: Lopes-da-Silva,Marcelo; Tonet,Gabriela E.L.; Vieira,Luiz G.E.
The greenbug Schizaphis graminum (Rondani) is one of the most important cereals pests in the world. Within populations of this species, several biotypes, which are clones that share same virulence relationships with cultivated plants, can be distinguished. Molecular markers have been used to genetically characterize insect populations because they are fast and cost effective. In order to find RAPD markers to identify Brazilian S. graminum biotypes, nineteen clonal populations of three biotypes (B, C and E) from Brazil and three clonal populations from the U.S. were examined. Eighteen primers were used to analyze the material, but only six primers revealed polymorphisms and among those, three produced diagnostic band profiles that allowed biotype...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Molecular marker; Greenbug; Insect-plant relationship.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000100009

Genetic evaluation of Eucalyptus camaldulensis in Mato Grosso State, Brazil
Provedor de dados: 190
Autores: Costa, Reginaldo Brito da; Azevedo, Luana Pâmella de Almeida; Martinez, Diego Tyszka; Tsukamoto Filho, Antonio de Arruda; Fernandes, Dayane Ávila; Oliveira, Oacy Eurico de; Resende, Marcos Deon Vilela de.
The study aimed to estimate parameters, values and genetic gain for growth traits in progenies of Eucalyptus camaldulensis, in the State of Mato Grosso. The progenies test was conducted under a randomized block design with 132 progenies, five replications and three plants in single rows, spaced 3 m x 2 m in Santo Antonio do Leverger, MT. At 24 months of age, progenies were evaluated for the characters: a) diameter at base height (DBH), in centimeters, b) total height, in meters, c) survival. The individual in the narrow sense heritability for the traits studied can be considered medium to high magnitude (0.13 and 0.21) for height and diameter, respectively. The heritability for survival were practically nil, due to low genetic variability among the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Genetic gain; Early selection; Mixed linear model Variabilidade genética; Ganho genético; Seleção precoce; Modelo linear misto.
Ano: 2012
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/337

Parâmetros genéticos para caracteres de crescimento inicial em progênies de bracatinga
Provedor de dados: 190
Autores: Menegatti, Renata Diane; Mantovani, Adelar; Navroski, Márcio Carlos.
Objetivou-se com este trabalho estimar parâmetros e valores genéticos para os caracteres diâmetro do coleto e altura de progênies de bracatinga em fase inicial de desenvolvimento. As sementes foram coletadas de 40 matrizes nativas nos municípios de Abelardo Luz, Chapadão do Lageado, Lages e Três Barras, SC. O teste de progênie foi conduzido na Fazenda Experimental do Centro de Ciências Agroveterinárias da Universidade do Estado de Santa Catarina, em Lages, visando à seleção de materiais genéticos adaptados ao local. O delineamento utilizado foi o de blocos ao acaso, com quarenta tratamentos (progênies), 30 repetições e dez plantas por parcela em linhas simples. Aos 12 meses de idade, foram avaliados os caracteres: diâmetro do coleto e altura total dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melhoramento Florestal; Engenharia Florestal; Genética Vegetal herdabilidade  melhoramento florestal  teste de progênies  variabilidade genética. Heritability; Tree breeding; Genetic variability.
Ano: 2016
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1003

Avaliação de progênies de macaúba na fase juvenil e estimativas de parâmetros genéticos e diversidade genética
Provedor de dados: 190
Autores: Manfio, Candida Elisa; Motoike, Sérgio Yoshimitsu; Resende, Marcos Deon Vilela de; Santos, Carlos Eduardo Magalhães dos; Sato, Aurora Yoshiko.
A macaúba (Acrocomia aculeata) é uma palmeira oleaginosa e o extrativismo de seus frutos é de grande importância socioeconômica para os pequenos produtores rurais. O objetivo deste trabalho foi avaliar a variabilidade genética e obter informações sobre o controle genético de características morfológicas relacionadas ao crescimento e vigor da macaúba. Foram coletadas 51 progênies em Minas Gerais e São Paulo, as quais são integrantes do banco ativo de germoplasma da Universidade Federal de Viçosa. Doze meses após o plantio, essas progênies foram avaliadas quanto ao crescimento da planta (cm) e número de folhas emitidas. Estas características apresentaram grande variabilidade entre as progênies. As estimativas dos coeficientes de herdabilidade foram de 87% e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrocomia aculeata; Variabilidade genética; Recursos genéticos; Herdabilidade Acroconia aculeata; Genetic variability; Genetic resources; Heritability.
Ano: 2012
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/283

Incorporation of compound inflorescences and selection of high-yielding progenies in cowpea
Provedor de dados: 86
Autores: Bernardo,Karla Annielle da Silva; Freire Filho,Francisco Rodrigues; Ribeiro,Valdenir Queiroz; Vieira,Paulo Fernando de Melo Jorge; Lopes,Ângela Celis de Almeida; Oliveira,Rosana Mendes de Moura.
Abstract: The objective of this work was to obtain cowpea (Vigna unguiculata) progenies with compound inflorescences, short peduncles, early cycle, good plant architecture, and high-yielding potential. The initial genetic material consisted of 453 F2:3 progenies, selected from ten crosses and evaluated using Federer’s augmented block design, with two controls. Of these, 60 progenies from the F4:5 and F4:6 generations were selected and assessed in two consecutive experiments, both in a 8x8 lattice design with four controls and four replicates. Early selection was efficient at fixing the evaluated traits. A wide genetic variability was detected among and within progenies. The estimated and obtained heritability and genetic gains in the F4:5 and F4:6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vigna unguiculata; Breeding; Genetic variability; Heritability; Quantitative genetics.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018001001150

Isozymic variability in a Brazilian collection of annatto (Bixa orellana L.)
Provedor de dados: 86
Autores: Carvalho,Jane Fiuza Rodrigues Portela de; Robinson,Ingrid Peters; Alfenas,Acelino Couto.
The objectives of this work were to optimize the isozyme electrophoresis technique for Bixa orellana, and use isozyme markers for a preliminary survey on the genetic variability in Brazilian annatto germplasm accessions. Collection consisted of seed samples from sixty open pollinated trees, representing two Northern and four Southern geographic provenances. The extraction, electrophoresis, and interpretation of annatto isozymes are described. Three out of the twenty-one identified isozyme loci were polymorphic in the collection. The percentage of polymorphic loci (P = 21.05) and the expected heterozygosity in annatto (H T = 0.064) were low, compared to other tropical woody species. A UPGMA phenogram, constructed with Nei's genetic distances, clearly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germplasm; Lipstick plant; Genetic variability; Allozyme; Phenogram.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000700005

Phytogeographical origin of Madeiran common beans based on phaseolin patterns
Provedor de dados: 86
Autores: Silva,Emanuel Marques da; Correia,Anísia Soraia Abreu; Lopes,Nuno Alexandre Amaral; Nóbrega,Humberto Gil Moreira; Ganança,José Filipe Teixeira; Domingues,Ana Maria; Khadem,Manhaz; Slaski,Jan Jacek; Carvalho,Miguel Ângelo Almeida Pinheiro de.
The objective of this work was to determine the geographic origin of the Madeiran common bean (Phaseolus vulgaris) gene pool. Phaseolin patterns of 50 accessions representing the diversity of common bean collected in Madeira, Portugal, and conserved in the ISOPlexis Germplasm Bank, were analysed using the Experion automated electrophoresis system, based on lab-on-a-chip technology. Five common bean standard varieties with typical phaseolin patterns were used to determine the phytogeographical origin of the Madeiran common bean accessions. Ninety two percent of the accessions exhibited a phaseolin pattern consistent with the one of common bean types belonging to the Andean gene pool, while the origin of the remaining 8% of the accessions was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Gene pool; Genetic variability; Germplasm; Lab-on-a-chip technology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000800012

Morphological, pedigree, and molecular distances and their association with hybrid wheat performance
Provedor de dados: 86
Autores: Bertan,Ivandro; Carvalho,Fernando Irajá Félix de; Oliveira,Antonio Costa de; Benin,Giovani; Vieira,Eduardo Alano; Valério,Igor Pirez.
The objectives of this work were to estimate the genetic distance among wheat genotypes using morphological, pedigree, molecular, and combined morphological and molecular measures, to determine the correlations between these measures, and to evaluate the combining ability of the genotypes. Three generations and two planting designs were studied. Six wheat genotypes were crossed using a diallel design. The F1, F2 and F3generations were evaluated in the field, in the crop seasons of 2003, 2004 and 2005, under spaced plant and full-row planting designs. The estimated general and specific combining abilities of tested hybrids were influenced both by the generation and the planting design. The correlation coefficients among the distance measures and between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Combining ability; Genetic variability; Parental selection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000200007

Selection of common bean lines with high agronomic performance and high calcium and iron concentrations
Provedor de dados: 86
Autores: Ribeiro,Nerinéia Dalfollo; Domingues,Lucas da Silva; Zemolin,Allan Emanoel Mezzomo; Possobom,Micheli Thaise Della Flora.
The objective of this work was to evaluate the genetic variability of common bean lines for cycle, weight of 100 grains, grain yield, cooking time, and grain calcium and iron concentrations. Twenty-four common bean lines were evaluated in two crop cycles (2010 and 2011). The ¯Z index was used for the selection of superior lines for most of the traits. The DF 06-19, DF 06-03, DF 06-17, DF 06-20, DF 06-11, DF 06-14, DF 06-01, DF 06-08, DF 06-22, and DF 06-04 lines showed high grain yield. All lines were of semi-early cycle and of fast cooking. The DF 06-08 and DF 06-23 lines showed high calcium concentration in grains (&gt;1.4 g kg-1 dry matter - DM), and the DF 06-09, DF 06-03, DF 06-04, and DF 06-06 lines presented high iron concentration in grains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Cooking time; Genetic variability; Genotype x environment interaction; Nutritional quality; ¯Z index.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013001000008

Molecular characterization of grapevine from Santa Catarina, Brazil, using microsatellite markers
Provedor de dados: 86
Autores: Schuck,Mariane Ruzza; Moreira,Flavia Maia; Guerra,Miguel Pedro; Voltolini,José Afonso; Grando,Maria Stella; Silva,Aparecido Lima da.
The objective of this work was to characterize the grape germplasm in Santa Catarina, Brazil, using microsatellite DNA markers (simple sequence repeats - SSR). The DNA samples were collected from leaves and shoots of accessions of public and private collections from the counties Urussanga, Nova Trento, Rodeio, São Joaquim, Campos Novos, Videira, and Água Doce. Ten SSR loci (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, VVMD31, and VVMD32) were analysed by capillary electrophoresis. Molecular profiling was conducted for 190 grapevines (European, American, and hybrids), and 67 genotypes were obtained. The data were compared with each other and with those from the literature and from online databases, in order to identify varieties and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis spp.; Cultivar identification; Genetic variability; Germplasm collection; Molecular markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000500008

Transgene inheritances and genetic similarities of near isogenic lines of genetically modified common beans
Provedor de dados: 86
Autores: Pinheiro,Patrícia Valle; Faria,Josias Corrêa de; Nogueira,Elsa Oliveira Paranaguá e Lago; Aragão,Francisco José Lima.
The objective of the present work was to determine the inheritance and stability of transgenes of a transgenic bean line expressing the genes rep-trap-ren from Bean golden mosaic virus and the bar gene. Crosses were done between the transgenic line and four commercial bean cultivars, followed by four backcrosses to the commercial cultivars. Progenies from each cross were evaluated for the presence of the transgenes by brushing the leaves with glufosinate ammonium and by polymerase chain reaction using specific oligonucleotides. Advanced generations were rub-inoculated with an isolate of Bean common mosaic necrosis virus (BCMNV). The transgenes were inherited consistently in a Mendelian pattern in the four crosses studied. The analyzed lines recovered close...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus; BCMNV; Genetic engineering; Genetic variability; Transgene genotypic stability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000900015

Genetic Dissimilarity in Conyza sumatrensis Revealed by Simple Sequence Repeat (SSR) Markers
Provedor de dados: 87
Autores: SCHNEIDER,T.; RIZZARDI,M.A.; BRAMMER,S.P.; SCHEFFER-BASSO,S.M.; NUNES,A.L.
ABSTRACT: In view of the rapid evolution of Conyza sumatrensis populations resistant to glyphosate, it is necessary to understand the genetic diversity aimed to improve strategies for managing this weed. We investigated the genetic dissimilarity among 15 biotypes of C. sumatrensis from different geographic regions using microsatellite loci. The biotypes, were cultivated in a greenhouse to obtain vegetal material for DNA extraction. Nineteen microsatellite markers (SSR), were developed for C. sumatrensis biotypes. The genetic dissimilarity was estimated by the Jaccard coefficient (JC) and the biotypes grouped by the UPGMA method. The results demonstrated a high dissimilarity (JC = 7.14 to 82.62) of the analyzed material, with the biotypes forming five...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; SSR; Tall fleabane; Genetic variability.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100218

Chemical Control and Morphoanatomical Analysis of Leaves of Different Populations of Sourgrass
Provedor de dados: 87
Autores: GOMES,L.J.P.; SANTOS,J.I.; GASPARINO,E.C.; CORREIA,N.M.
ABSTRACT Digitaria insularis has been selected by frequent glyphosate applications both in no-till areas and in fruit orchards. The objective of this paper was to evaluate control efficacy of D. insularis populations by glyphosate, alone and mixed with quizalofop-p-tefuryl, as well as classify them for herbicide sensitivity as susceptible, moderately susceptible and tolerant. This study also aimed to evaluate the relations between herbicide sensitivity and morphoanatomic features of leaf tissues. For chemical control, the experiment was conducted in a completely randomized design, with a 12 x 4 factorial arrangement with four replications. Twelve populations of D. insularis were treated with glyphosate alone at two concentrations (1.44 and 2.16 kg a.e....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Digitaria insularis; Glyphosate; Tolerance; Genetic variability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100202

Chemical control of different Digitaria insularis populations and management of a glyphosate-resistant population
Provedor de dados: 87
Autores: CORREIA,N.M.; ACRA,L.T.; BALIEIRO,G.
This study aimed to control different populations of Digitaria insularisby glyphosate herbicide, isolated and mixed, besides the combination of methods (chemical and mechanical) to manage resistant adult plants. Three experiments were conducted, one in pots which were maintained under non-controlled conditions and two under field conditions. In the experiment in pots, twelve populations of D. insularis were sprayed with isolated glyphosate (1.44 and 2.16 kg a.e. ha-1) and mixed (1.44 and 2.16 kg a.e. ha-1) with quizalofop-p tefuryl (0.12 kg i.a. ha-1). The treatment of 1.44 kg a.e. ha-1 of glyphosate plus 0.12 kg a.i. ha-1 of quizalofop was sufficient for adequate control (&gt;95%) of all populations. Population 11 (area of grain production in Itumbiara,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Desiccation; Glyphosate; Tolerance; Genetic variability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000100093

Evidence of natural hybridization and introgression in Bulbophyllum involutum Borba, Semir &amp;amp; F. Barros and B. weddellii (Lindl.) Rchb. f. (Orchidaceae) in the Chapada Diamantina, Brazil, by using allozyme markers
Provedor de dados: 88
Autores: Azevedo,Cecília O.; Borba,Eduardo L.; Van Den Berg,Cássio.
Hybridization between B. involutum and B. weddellii (Orchidaceae) has been first observed in the Serra do Cipó, Minas Gerais State, Brazil, the hybrid being described as B. ×cipoense Borba &amp;amp; Semir. In this study, allozime electrophoresis was used to test the hypothesis of occurrence of hybridization between these two species, as suggested by morphological characters, in the Chapada Diamantina, Bahia State, Brazil. The lack of a diagnostic locus does not allow definite confirmation of the natural hybridization, although this hypotheses is reinforced by the absence of exclusive alleles in the putative hybrid individuals. The existence of several different genotypes points out to either population derived from multiple hybridization events or the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bulbophyllum; Genetic variability; Hybridization; Introgression; Orchidaceae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042006000300008

Variabilidade intra-específica em cinco populações de Oncidium varicosum Lindl. (Orchidaceae -- Oncidiinae) em Minas Gerais
Provedor de dados: 88
Autores: CARDIM,DARUSKA CAVALCANTE; CARLINI-GARCIA,LUCIANA APARECIDA; MONDIN,MATEUS; MARTINS,MARINA; VEASEY,ELIZABETH ANN; ANDO,AKIHIKO.
Com o objetivo de estudar a variabilidade genética intra-específica em Oncidium varicosum Lindl. (Orchidaceae - Oncidiinae), foram avaliadas cinco populações de Minas Gerais, coletadas em Alfenas (AL), Estiva (ES), Pedra do Coração (PC), Pedra Branca (PB) e Pouso Alegre (PA). Foram considerados 22 caracteres florais, medidos a partir de "vouchers" (fichas florais), empregando-se métodos estatísticos univariados e multivariados (análise discriminante canônica - CDA e análise de agrupamento a partir de distância de Mahalanobis). Houve diferença significativa (p &lt; 0,05) entre populações, pelo teste F, para 13 caracteres. O primeiro eixo canônico separou a população PA de PB e de PC. Os indivíduos de PA são menores que os de PC, que são menores que os de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orchidaceae; Oncidium varicosum; Morphometric analysis; Genetic variability; Multivariate analysis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000500010

Leaf area of common bean genotypes during early pod filling as related to plant adaptation to limited phosphorus supply
Provedor de dados: 90
Autores: Trindade,Roberto Santos; Araújo,Adelson Paulo; Teixeira,Marcelo Grandi.
Low phosphorus supply markedly limits leaf growth and genotypes able to maintain adequate leaf area at low P could adapt better to limited-P conditions. This work aimed to investigate the relationship between leaf area production of common bean (Phaseolus vulgaris) genotypes during early pod filling and plant adaptation to limited P supply. Twenty-four genotypes, comprised of the four growth habits in the species and two weedy accessions, were grown at two P level applied to the soil (20 and 80 mg kg-1) in 4 kg pots and harvested at two growth stages (pod setting and early pod filling). High P level markedly increased the leaf number and leaf size (leaf area per leaf), slightly increased specific leaf area but did not affect the net assimilation rate. At...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Growth habit; Phaseolus vulgaris; Phosphorus efficiency; Plant growth analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000100012

Isolation, bioprospecting and diversity of salt-tolerant bacteria associated with sugarcane in soils of Pernambuco, Brazil
Provedor de dados: 91
Autores: Leite,Maria C. B. S.; Farias,Andreza R. B. de; Freire,Fernando J.; Andreote,Fernando D.; Kuklinsky-Sobral,Júlia; Freire,Maria B. G. S.
ABSTRACT The selection of salinity tolerant bacteria may provide greater promotion of plant growth in soils with salt stress. This study aimed to isolate and select bacterial isolates of sugarcane, tolerant to salinity and plant growth promoters, and assess genetic diversity. To do so, endophytic root and rhizosphere of sugarcane, were isolated in culture medium with and without NaCl. The bacterial population density, the potential for biological nitrogen fixation (BNF), production of indole acetic acid (IAA), inorganic phosphate solubilization and quorum sensing molecule production were evaluated. Furthermore, the genetic diversity was assessed by Denaturing Gradient Gel Electrophoresis (DGGE) of nifH. Salinity tolerance tests were also conducted. There...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic; Genetic variability; Plant growth promotion.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662014001300073

Genetic variability of a population of Aedes aegypti from Paraná, Brazil, using the mitochondrial ND4 gene
Provedor de dados: 92
Autores: Twerdochlib,Adriana L.; Bonna,Ana C. Dalla; Leite,Selene S.; Chitolina,Rodrigo F.; Westphal,Betina; Navarro-Silva,Mario A.
Genetic variability of a population of Aedes aegypti from Paraná, Brazil, using the mitochondrial ND4 gene. To analyze the genetic variability of populations of Aedes aegypti, 156 samples were collected from 10 municipalities in the state of Paraná, Brazil. A 311 base pairs (bp) region of the NADH dehydrogenase subunit 4 (ND4) mitochondrial gene was examined. An analysis of this fragment identified eight distinct haplotypes. The mean genetic diversity was high (h = 0.702; p = 0.01556). AMOVA analysis indicated that most of the variation (67%) occurred within populations and the F ST value (0.32996) was highly significant. F ST values were significant in most comparisons among cities. The isolation by distance was not significant (r = -0.1216 and p = 0,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aedes aegypti; Genetic variability; Mitochondrial DNA; Genetic population; State of Paraná.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262012000200013

PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae)
Provedor de dados: 92
Autores: Calado,Daniéla; Navarro-Silva,Mario Antonio; Sallum,Maria Anice Mureb.
The aim of the present study was to examine genetic variability in populations of An. cruzii by employing PCR-RAPD and PCR-RFLP markers. All analyses were carried out using individuals of the F1 generation of wild caught females obtained in Santa Catarina State (Florianópolis and São Francisco do Sul), Paraná State (Morretes, Paranaguá and Guaratuba) and São Paulo State (Cananéia). In the PCR-RAPD experiments, seven primers were used for comparisons within and among populations. The restriction profile of the ITS2 including a fragment of both 5.8S and 28S regions of the rDNA was obtained with the enzymes BstUI, HaeIII, TaqI, HhaI, Sau96I, HinfI, HincII and NruI. The PCR-RAPD method detected a large number of polymorphic bands. Genetic distance among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anophelinae; Genetic variability; ITS2; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262006000300014

Genetic polymorphism among natural populations of Anacardium humile A. ST-HIL
Provedor de dados: 94
Autores: Santos,Silvia Correa; Carvalho,Raquel dos Santos; Davide,Livia Maria Chamma.
Abstract  The genus Anacardium presents nine species, of these, three have sub-bush size, common in the Cerrado of the Center-West of Brazil. The objective of this work was to evaluate the genetic variability of the species, collected in eleven provenances, using RAPD markers. Genomic DNA from 122 accessions was extracted and amplified with 25 decamer primers. The results indicated polymorphism, ranging from 77.71% to 96.18%. The distribution of genetic diversity among and within populations shows that 27.14% of the variability is found between populations and 37.44% within the populations, suggesting the existence of genetic variability that may be related to the reproductive strategies adopted by the species throughout its evolution. The index of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Genetic variability; Diversity; Native species; Cerrado.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452020000100802

Diversity and genetic structure in natural populations of Hancornia speciosa var. speciosa Gomes in northeastern Brazil
Provedor de dados: 94
Autores: Martins,Georgia Vilela; Martins,Luiza Suely Semen; Veasey,Elizabeth Ann; Lederman,Ildo Eliezer; Silva,Edson Ferreira da.
Hancornia speciosa Gomes is a fruit tree native from Brazil that belongs to Apocinaceae family, and is popularly known as Mangabeira. Its fruits are widely consumed raw or processed as fruit jam, juices and ice creams, which have made it a target of intense exploitation. The extractive activities and intense human activity on the environment of natural occurrence of H. speciosa has caused genetic erosion in the species and little is known about the ecology or genetic structure of natural populations. The objective of this research was the evaluation of the genetic diversity and genetic structure of H. speciosa var. speciosa. The genetic variability was assessed using 11 allozyme loci with a sample of 164 individuals distributed in six natural populations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Gene flow; Genetic resources; Genetic variability; Isozyme; Mangabeira.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452012000400023

Repeatability of morpho-agronomic characters of Theobroma grandiflorum fruits
Provedor de dados: 94
Autores: Alcoforado,Ayulle Thalía Watson; Pedrozo,Cássia Ângela; Mayer,Marcos Miguel; Lima-Primo,Hyanameyka Evangelista de.
Abstract  The objective of this study was to estimate coefficients of repeatability, to determine the minimum number of fruits necessary for genotype selection and to estimate phenotypic correlation coefficients between morpho-agronomic characters of Theobroma grandiflorum fruits. It was evaluated twenty - two genotypes of an agroforestry system implanted in the municipality of Cantá - RR. Ten fruits per genotype were collected and evaluated for longitudinal length, transverse diameter, total weight, peel thickness, peel weight, pulp weight, placental weight and wet seed weight. Four statistical methods were used to estimate the repeatability coefficients: variance analysis, main components based on the correlation matrix and the covariance matrix, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Selection of genotypes; Phenotypic correlation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000200801

Germplasm characterization of three jabuticaba tree species
Provedor de dados: 94
Autores: Danner,Moeses Andrigo; Citadin,Idemir; Sasso,Simone Aparecida Zolet; Sachet,Marcos Robson; Mazaro,Sérgio Miguel.
The purpose of this study was to characterize cultivated genotypes of three jabuticaba species (Plinia cauliflora, P. trunciflora, and P. jaboticaba). Phenology and fruit growth, as well as leaf, flower and fruit traits were evaluated. Variability in all traits was observed among genotypes of the three jabuticaba species. The trait peduncle size is indicated for differentiation of the three species under study. The leaf and fruit sizes of the genotypes P. trunciflora 3, P. trunciflora 4, P. trunciflora 5 and P. jaboticaba 1 differ from those described in the literature for these species, indicating the formation of ecotypes. Jabuticaba fruit skin contains high anthocyanin and flavonoid concentrations, with potential use in food and pharmaceutical industries.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plinia sp; Germplasm bank; Genetic variability; Fruit quality.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000300019

HETEROTIC GROUP FORMATION IN PSIDIUM GUAJAVA L. BY ARTIFICIAL NEURAL NETWORK AND DISCRIMINANT ANALYSIS
Provedor de dados: 94
Autores: CAMPOS,BIANCA MACHADO; VIANA,ALEXANDRE PIO; QUINTAL,SILVANA SILVA RED; BARBOSA,CIBELLE DEGEL; DAHER,ROGÉRIO FIGUEIREDO.
ABSTRACT  The present study aimed at evaluating the heterotic group formation in guava based on quantitative descriptors and using artificial neural network (ANN). For such, we evaluated eight quantitative descriptors. Large genetic variability was found for the eight quantitative traits in the 138 genotypes of guava. The artificial neural network technique determined that the optimal number of groups was three. The grouping consistency was determined by linear discriminant analysis, which obtained classification percentage of the groups, with a value of 86 %. It was concluded that the artificial neural network method is effective to detect genetic divergence and heterotic group formation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Guava; Genetic variability; Multivariate analysis; Heterotic group.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000100151

Managing genetic diversity and society needs
Provedor de dados: 96
Autores: Mariante,Arthur da Silva; Egito,Andréa Alves; Albuquerque,Maria do Socorro Maués; Paiva,Samuel Rezende; Ramos,Alexandre Floriani.
Most livestock are not indigenous to Brazil. Several animal species were considered domesticated in the pre-colonial period, since the indigenous people manage them as would be typical of European livestock production. For over 500 years there have been periodic introductions resulting in the wide range of genetic diversity that for centuries supported domestic animal production in the country. Even though these naturalized breeds have acquired adaptive traits after centuries of natural selection, they have been gradually replaced by exotic breeds, to such an extent, that today they are in danger of extinction To avoid further loss of this important genetic material, in 1983 Embrapa Genetic Resources and Biotechnology decided to include conservation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal genetic resources; Brazilian conservation program; Genetic variability; Microsatellites.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982008001300016

Behavior of genetic (co)variance components in populations simulated from non-additive genetic models of dominance and overdominance
Provedor de dados: 96
Autores: Cunha,Elizângela Emídio; Euclydes,Ricardo Frederico; Lopes,Paulo Sávio; Torres,Robledo de Almeida; Carneiro,Paulo Luiz Souza.
The aim of this work was to investigate the short-term behavior of the genetic variability of quantitative traits simulated from models with additive and non-additive gene action in control and phenotypic selection populations. Both traits, one with low (h² = 0.10) and the other with high (h² = 0.60) heritability, were controlled by 600 biallelic loci. From a standard genome, it was obtained six genetic models which included the following: only the additive gene effects; complete and positive dominance for 25, 50, 75 and 100% of the loci; and positive overdominance for 50% of the loci. In the models with dominance deviation, the additive allelic effects were also included for 100% of the loci. Genetic variability was quantified from generation to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Closed population; Computer simulation; Dominance deviation; Genetic variability; Intralocus interaction.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000900013

Patterns of genetic diversity of local pig populations in the State of Pernambuco, Brazil
Provedor de dados: 96
Autores: Silva,Elizabete Cristina da; Dutra Junior,Wilson Moreira; Ianella,Patrícia; Gomes Filho,Manoel Adrião; Oliveira,Cláudio José Parro de; Ferreira,Débora Nathália de Moura; Caetano,Alexandre Rodrigues; Paiva,Samuel Rezende.
This study estimated the genetic diversity and structure of 12 genetic groups (GG) of locally adapted and specialized pigs in the state of Pernambuco using 22 microsatellite markers. Nine locally adapted breeds (Baé, Caruncho, Canastra, Canastrão, Mamelado, Moura, Nilo, Piau and UDB (Undefined Breed)) and 3 specialized breeds (Duroc, Landrace and Large White), totaling 190 animals, were analyzed. The Analysis of Molecular Variance (AMOVA) showed that 3.2% of the total variation was due to differences between genetic groups, and 3.6% to differences between local and commercial pigs. One hundred and ninety eight alleles were identified and apart from the Large White breed, all GG presented Hardy-Weinberg Equilibrium deviations for some loci. The total and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal genetics resources; Genetic variability; Microsatellites loci; Sus Scrofa.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000800010

Multivariate approach in the selection of superior soybean progeny which carry the RR gene
Provedor de dados: 124
Autores: Dallastra,Anderson; Unêda-Trevisoli,Sandra Helena; Ferraudo,Antonio Sergio; Di Mauro,Antonio Orlando.
Efficiency in the use of genetic variability, whether existing or created, increases when properly explored and analysed. Incorporation of biotechnology into breeding programs has been the general practice. The challenge for the researcher is the constant development of new and improved cultivars. The aim of this experiment was to select progenies with superior characteristics, whether or not carriers of the RR gene, derived from bi-parental crosses in the soybean, with the help of multivariate techniques. The experiment was carried out in a family-type experimental design, including controls, during the agricultural year 2010/2011 and 2011/2012 in Jaboticabal in the Brazilian State of São Paulo. From the F3 generation, phenotypically superior plants were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Genetic variability; Multivariate analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000300021

Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs
Provedor de dados: 124
Autores: Freitas,Suzane Fonseca; Moreira,Heden Luiz Marques; Souza,Daiane Machado; Moreira,Carla Giovane Ávila; Tavares,Rafael Aldrighi; Dionello,Nelson José Laurino.
ABSTRACT This study aimed to develop PCR assays, reaction combinations, and validation of tetranucleotide SSR loci for tilapia, to minimize the impact of erroneous allele inferences on genotype determination of this marker. Microsatellites containing tetranucleotide repeats were obtained from tilapia genome, version 2.1, avoiding loci in the same linking group. Primers were designed for different fragment sizes, and fluorescence added to each locus. A total of 10 loci were amplified, separately and in combination, and loaded into a single capillary sequencer panel. Alleles were amplified without stutters and easily interpreted. PCR amplifications of DNA repeatedly extracted from samples, and genotyping at different PCR rounds were performed to infer allele...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Genotyping; Microsatellite markers.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400409

Genetic tolerance to low temperatures in irrigated rice
Provedor de dados: 124
Autores: Streck,Eduardo Anibele; Aguiar,Gabriel Almeida; Silva,Pedro Ujacov da; Fronza,Rafael Tobias Lang; Magalhães Júnior,Ariano Martins de.
ABSTRACT The aim of this study was to characterise genetic variability to low-temperature tolerance in the emergence stage of irrigated-rice genotypes under controlled and field conditions. Thirty-seven genotypes were evaluated, interspersed with controls of different levels of low-temperature tolerance. The design used was of randomised blocks with three replications. The genotypes were submitted to germination and emergence in controlled-temperature trials at 13 ºC (Trial I) and 17 ºC (Trial II), and in field environments with no cover (Trial III) and with a cover of polyethylene (Trial IV), where the soil temperature was monitored. The evaluations were carried out daily by counting the emerged seedlings and later inferring the speed of emergence index...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa L.; Abiotic stress; Cold; Genetic variability.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000300416

Agro-morphological characterization of upland rice accessions
Provedor de dados: 63
Autores: Nascimento,Wellington Ferreira do; Silva,Edson Ferreira da; Veasey,Elizabeth Ann.
The agro-morphological characterization is fundamental in order to provide information for plant breeding programs. The aim of the present study was to characterize 146 accessions of upland rice (Oryza sativa L.), based on qualitative and quantitative agro-morphological descriptors. The experiment was conducted in Recife, state of Pernambuco, Brazil, using a randomized block design with three replicates. Polymorphism was observed among 12 of 14 qualitative characters evaluated, whereas significant differences (p &lt; 0.05) were observed for 11 of the 14 analysed quantitative traits. Genetic variance was higher than environmental variance and the average inheritability coefficients were above 80 % for all characters, which ensures the predominance of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Germplasm; Ex-situ conservation; Pre-breeding.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000600008

Aggressiveness between genetic groups I and II of isolates of Cercospora zeae-maydis
Provedor de dados: 63
Autores: Mathioni,Sandra Marisa; Carvalho,; Brunelli,Kátia Regiane; Beló,André; Camargo,Luis Eduardo Aranha.
For many years, the gray leaf spot disease (GLS) caused by the fungus Cercospora zeae-maydis Tehon &amp;amp; Daniels, was not considered an important pathogen of maize (Zea mays, L.) in Brazil. However, the recent adoption of agronomical practices such as no-tillage and cultivation under central pivot irrigation systems increased the incidence and severity to the extent that GLS is now one of the most important diseases of maize. Isolates of C. zeae-maydis can be distinguished by two genetic groups (I and II) based on AFLP markers and on polymorphisms of the ITS and 5.8S rDNA regions. Until now, however, the biological implications of this distinction remain unclear. This study investigated whether isolates from the two genetic groups differ in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disease resistance; Gray leaf spot; Genetic variability; Pathogenicity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000600006

Isoenzymatic variability of cassava accessions from different regions in Brazil
Provedor de dados: 63
Autores: Cabral,Betânia Lúcia Rocha; Souza,Joelson Araújo de; Ando,Akihiko; Veasey,Elizabeth Ann; Cardoso,Eloisa Maria Ramos.
Cassava (Manihot esculenta Crantz) belongs to the Euphorbiaceae family, and is widely cultivated in Brazil. The objective of this study was to evaluate the isoenzymatic variability of 200 cassava accessions from the germplasm bank of Embrapa Amazonia Oriental. Seven groups were formed according to their origin: 1-Amazonas, 2-Amapa, 3-Bahia, 4-Para, 5-Rondonia, 6-Various, for accessions with a maximum of three individuals per place of origin, and 7 - Accessions of indefinite origin. The accessions were also evaluated as a whole. For the electrophoretic analyses, samples of young leaves were used in a 12% starch gel. Eight isoenzymatic systems were evaluated: acid phosphatase (ACP), leucine aminopeptidase (LAP), glucose-6-phosphate dehydrogenase (G6PDH),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Genetic variability; Germplasm bank; Isozymes.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000300017

Recurrent mass selection for upright plant architecture in common bean
Provedor de dados: 63
Autores: Pires,Luiz Paulo Miranda; Ramalho,Magno Antonio Patto; Abreu,Ângela de Fátima Barbosa; Ferreira,Monica Christina.
Plant with a more upright architecture offers many advantages to farmers. Recurrent mass selection (RS) programs for carioca type common bean have been implemented for the purpose of obtaining new lines that will generate the high yields that are associated with upright plant archi tecture. This study aimed to assess the efficiency of recurrent mass selection (RS) for upright plant architecture in common bean (Phaseolus vulgaris) and the effect of RS on grain yield and to verify whether or not there is still variability in the population that favors continuing selection programs, using information obtained from progenies evaluated in cycle five (CV) and cycle eight (CVIII) of the RS program. Mass selection for more upright plants was performed visually in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant Breeding; Genetic progress; Genetic variability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000300009

Morphoagronomic variation in natural populations of Trifolium riograndense (Burkart)
Provedor de dados: 63
Autores: Conterato,Ionara Fatima; Dall'Agnol,Miguel; Schifino-Wittmann,Maria Teresa; Montardo,Daniel Portela; Pontalti,Gabriel Colombo; Almeida,Danielle.
Several species of Trifolium are widely cultivated as forage for animals and green manure. This work aimed to characterize and estimate through morphological and agronomic traits the genetic diversity of natural populations of T. riograndense, an important forage legume from native pastures of the state of Rio Grande do Sul, Brazil. Plant height and diameter, number of primary and secondary stolons, stolon length, number of nodes per primary stolon, internode length, primary stolon diameter, leaf area, petiole length and dry matter production were analyzed in 29 accessions. The first five attributes and dry matter production were evaluated twice. A broad phenotypic variability was observed for almost all characteristics. Positive correlations occurred...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Forage legume; Genetic variability; Morphology; Plant breeding.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000600009

Quantitative variation in the tropical maize population, ESALQ-PB1
Provedor de dados: 63
Autores: Andrade,João Antonio da Costa; Miranda Filho,José Branco de.
Good yield and intermediate plant height, ear height, and tassel size characterize the maize population ESALQ-PB1 as an outstanding breeding population. Estimates of genetic parameters are reported for 13 traits: plant height (PH), ear height (EH), ear placement (EP), tassel length (TL), tassel weight (TW), tassel branch number (TB), ear weight (EW), total grain weight (GW), ear length (EL), ear diameter (ED), kernel row number (RN), kernel number per row (KR) and prolificacy (PR). Results refer to one location and one year. Genetic variation was detected for all traits, and the estimates of the additive genetic variance are presented. The coefficients of heritability (individual basis) varied from 0.14 to 0.72 and were considered high for PH, EH and TB;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic parameters; Genetic variability; Heritability; Recurrent selection.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000200011

Root phosphatase activity, plant growth and phosphorus accumulation of maize genotypes
Provedor de dados: 63
Autores: Machado,Cynthia Torres de Toledo; Furlani,Ângela Maria Cangiani.
The activity of the enzyme phosphatase (P-ase) is a physiological characteristic related to plant efficiency in relation to P acquisition and utilization, and is genetically variable. As part of a study on maize genotype characterization in relation to phosphorus (P) uptake and utilization efficiency, two experiments were set up to measure phosphatase (P-ase) activity in intact roots of six local and improved maize varieties and two sub-populations. Plants were grown at one P level in nutrient solution (4 mg L-1) and the P-ase activity assay was run using 17-day-old plants for varieties and 24-day-old plants for subpopulations. Shoot and root dry matter yields and P concentrations and contents in plant parts were determined, as well as P-efficiency...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays L.; Genetic variability; Enzymatic activity; Nutritional efficiency.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000200015

Resistance to Pratylenchus brachyurus in Vitis species population through multivariate approaches and mixed models
Provedor de dados: 63
Autores: Santos,Paulo Ricardo dos; Viana,Alexandre Pio; Gomes,Vicente Martins; Preisigke,Sandra da Costa; Almeida,Odimar Ferreira de; Santos,Eileen Azevedo; Rodrigues,Daniele Lima; Rodrigues,Rosana; Walker,Michael Andrew.
ABSTRACT: Genetically diverse interspecific hybrids of Vitis were selected for resistance to Pratylenchus brachyurus. Three segregating populations with 57 hybrid crosses were evaluated. The parents included Vitis romanetiiC166-043 × 07355-075, 06354-047 × Cereza and 06354-047 × Nocera, selected from the germplasm bank at the University of California, Davis, the United States. The experiment was arranged in a randomized block design, with three replications and three plants per plot. Root mass, nematodes per gram of root and reproduction factor were determined and used as quantitative variables; 16 multi categoric descriptors were also evaluated. The traits were analyzed using the Ward-Modified Location Model procedure (Ward-MLM) for the composition of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis genotypes; Nematode; Root; Genetic variability.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001500424

Kinetics of phosphorus uptake and root morphology of local and improved varieties of maize
Provedor de dados: 63
Autores: Machado,Cynthia Torres de Toledo; Furlani,Ângela Maria Cangiani.
Interspecific and intraspecific differences in the efficiency of utilization of soil phosphorus (P) are explained, in part, by plant morphological and physiological variations, which characterize the plant for nutrient acquisition. As part of a study on maize genotypes characterization for P-uptake and use efficiency, kinetic parameters of P uptake and root morphological characters were evaluated for six local and improved maize varieties, grown in nutrient solution. The kinetic parameters related to P influx were: maximal transport rate (Vmax), the Michaelis-Menten constant (Km), and the external concentration when net uptake is zero (Cmin). Measured root morphological characters were: root length, radius, and surface area. Shoot and root dry matter yield...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Phopshorus acquisition; Kinetics parameters; Root system; Nutritional efficiency.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000100012

Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil
Provedor de dados: 63
Autores: Povh,Jayme Aparecido; Ribeiro,Ricardo Pereira; Lopera-Barrero,Nelson Mauricio; Jacometo,Carolina Bespalhok; Vargas,Lauro; Gomes,Patrícia Cristina; Lopes,Taís da Silva.
Monitoring the genetic diversity has fundamental importance for fish stocking programs. This experiment aims to evaluate the genetic diversity in two hatchery stations (A and B) with pacu Piaractus mesopotamicus (Holmberg, 1887) in Andirá, state of Paraná, Brazil used in stocking programs of Paranapanema River. Six microsatellite loci were amplified using DNA extracted from 60 fin-clipping samples. The broodstock B had the average number of alleles and the mean heterozygosity (alleles: 3.7 and H O: 0.628) higher than the broodstock A (alleles: 3.5 and H O: 0.600). Alleles with low frequency levels were observed in the both broodstocks. The positive coefficients of endogamy in the locus Pme2 (broodstock A: F IS = 0.30 and broodstock B: F IS = 0.20), Pme5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piaractus mesopotamicus; Genetic variability; Hatchery stations; Molecular markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300006

Isoenzymatic variability in tropical maize populations under reciprocal recurrent selection
Provedor de dados: 63
Autores: Pinto,Luciana Rossini; Vieira,Maria Lúcia Carneiro; Souza Jr.,Cláudio Lopes de; Silva,Rainério Meireles da.
Maize (Zea mays L.) is one of the crops in which the genetic variability has been extensively studied at isoenzymatic loci. The genetic variability of the maize populations BR-105 and BR-106, and the synthetics IG-3 and IG-4, obtained after one cycle of a high-intensity reciprocal recurrent selection (RRS), was investigated at seven isoenzymatic loci. A total of twenty alleles were identified, and most of the private alleles were found in the BR-106 population. One cycle of reciprocal recurrent selection (RRS) caused reductions of 12% in the number of alleles in both populations. Changes in allele frequencies were also observed between populations and synthetics, mainly for the Est 2 locus. Populations presented similar values for the number of alleles per...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Genetic markers; Genetic variability; Isozymes; Population improvement.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000200013

PCR amplification and sequence analyses of reverse transcriptase-like genes in Crinipellis perniciosa isolates
Provedor de dados: 102
Autores: Pereira,Jorge F.; Ignacchiti,Mariana D.C.; Araújo,Elza F.; Brommonschenkel,Sérgio H.; Cascardo,Júlio C.M.; Pereira,Gonçalo A. G.; Queiroz,Marisa V.
Reverse transcriptase (RT) sequence analysis is an important technique used to detect the presence of transposable elements in a genome. Putative RT sequences were analyzed in the genome of the pathogenic fungus C. perniciosa, the causal agent of witches' broom disease of cocoa. A 394 bp fragment was amplified from genomic DNA of different isolates of C. perniciosa belonging to C-, L-, and S-biotypes and collected from various geographical areas. The cleavage of PCR products with restriction enzymes and the sequencing of various RT fragments indicated the presence of several sequences showing transition events (G:C to A:T). Southern blot analysis revealed high copy numbers of RT signals, forming different patterns among C-, S-, and L-biotype isolates....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Transposable elements; Witches' broom; Theobroma cacao.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500001

Transferability of heterologous primers in Brycon falcatus
Provedor de dados: 36
Autores: PENHA,Diego dos Santos; SOUZA,Felipe Pinheiro de; LIMA,Ed Christian Suzuki de; URREA-ROJAS,Angela Maria; PANDOLFI,Victor César Freitas; YAMACHITA,Andrei Lincoln; POVH,Jayme Aparecido; LEITE,Natalia Gonçalves; PEREIRA,Ulisses de Pádua; LOPERA-BARRERO,Nelson Mauricio.
ABSTRACT The genusBryconcomprises fish species of significant socioeconomic and biological importance in Brazil. Despite that, the genetic knowledge about these species is scarce, especially regardingBrycon falcatus. Thus, the objective of this study was to evaluate the transferability of heterologous microsatellite primers inB. falcatus for the first time. Heterologous primers obtained from B. opalinus, B. hilarii, B. insignis, B. orbignyanus, B. amazonicus, Prochilodus argenteus, Prochilodus lineatus, Piaractus mesopotamicus, and Colossoma macropomum were evaluated. The primers that showed the best amplification patterns were applied to a sample of 22 individuals and the genetic parameters were calculated. Nine primers displayed satisfactory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bryconidae; Cross amplification; Genetic conservation; Genetic variability; SSR.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300232

A NEW WORLD MONKEY MICROSATELLITE (AP74) HIGHLY CONSERVED IN PRIMATES
Provedor de dados: 37
Autores: OKLANDER,LUCIANA INÉS; STEINBERG3,ELIANA RUTH; DOLORES MUDRY,MARTA.
Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primates; Microsatellite markers; PCR amplification; Non-invasive sampling; Genomic conservation; Genetic variability.
Ano: 2012
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000100007

Use of microsatellite markers to assess the identity and genetic diversity of Vitis labrusca and Vitis rotundifolia cultivars
Provedor de dados: 1
Autores: Schuck, Mariane Ruzza; Universidade Federal do Paraná; Biasi, Luiz Antonio; Universidade Federal do Paraná; Moreira, Flavia Maia; Instituto Federal de Educação, Ciência e Tecnologia de Santa Catarina; Silva, Aparecido Lima da; Universidade Federal de Santa Catarina; Riaz, Summaira; University of California; Walker, Michael Andrew; University of California.
Ten grapevine cultivars were genotyped at eight microsatellite loci to characterize their identity and genetic diversity. Of these, nine cultivar profiles matched with those of databases and ‘Magoon’ matched with ‘Regale’ in the present study and ‘Regale’ in the University of California (Davis) database, implicating a likely error in planting. The number of alleles ranged from 5 (VVM5) to 9 (VVMD31), and the observed heterozygosity ranged from 37.14 (VVMD5) to 97.14% (VVMD27), with no significant differences in relation to the expected values for any of the loci, with the exception of VVMD5. The polymorphism information content values were observed to be above 0.25 in more than 85% of the loci analyzed, and VVMD31 was the most informative. The UPGMA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agronomia; Melhoramento Vegetal; Fitotecnia grapevine; Genetic variability; Molecular marker Melhoramento Vegetal.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/17869

Marcadores moleculares RAPD e descritores morfológicos na avaliação da diversidade genética de goiabeiras (Psidium guajava L.) - doi: 10.4025/actasciagron.v32i4.4720
Provedor de dados: 1
Autores: Gomes Filho, Aroldo; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF; Oliveira, Jurandi Gonçalves de; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF; Viana, Alexandre Pio; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF; Siqueira, Ana Paula de Oliveira; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF; Oliveira, Marcos Góes; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF; Pereira, Messias Gonzaga; Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF.
O conhecimento da variabilidade genética e fenotípica entre diferentes acessos de goiabeiras é importante para se apoiar programas de melhoramento dessa espécie na região Norte Fluminense que carece de novas culturas capazes de gerar renda aos produtores locais. O objetivo deste trabalho foi avaliar a divergência genética entre seis cultivares e 19 acessos de goiabeiras, por meio de marcadores moleculares RAPD e características morfoagronômicas. Foram obtidas 117 marcas polimórficas, utilizando-se 28 iniciadores. Os resultados mostraram uma concordância parcial entre os métodos de agrupamentos estudados, com a formação de 12 grupos. O acesso Vita 3 e o acesso 6 foram os mais divergentes, apresentando distância genética de 0,663. A análise comparativa dos...
Tipo: Pesquisa de campo e laboratório
Palavras-chave: 5.01.03.05-9 Polimorfismo; Variabilidade genética; Método de otimização de Tocher; Método hierárquico UPGMA Melhoramento Vegetal Polymorphic; Genetic variability; Tocher’s optimization method; UPGMA cluster analysis.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/4720

Genetic diversity among Brazilian okra landraces detected by morphoagronomic and molecular descriptors
Provedor de dados: 1
Autores: Massucato, Luana Rainieri; Nakamura, Karina Kazue; Ruas, Paulo Mauricio; Zefa, Douglas Mariani; Silva, Derly José Henrique da; Gonçalves, Leandro Simões Azeredo.
The conservation of okra landraces [Abelmoschus esculentus (L.) Moench] in gene banks is essential for the success of their use in breeding programmes. This study evaluated the genetic diversity among okra landraces in Brazil based on morphoagronomic descriptors and AFLP markers. We studied 30 accessions of the vegetable gene bank of the Universidade Federal de Viçosa. To this end, 17 morphoagronomic descriptors and five combinations of AFLP primers were used. Genetic parameters were estimated for the quantitative traits and the accessions were grouped by Ward’s method, using the Gower’s and Jaccard’s distance measures, respectively, for the morphoagronomic and molecular data. Polymorphisms were observed for all qualitative traits, while the quantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Molecular markers; Phenotype; Bayesian analysis.; Recursos Genéticos.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/43426

A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.) - doi:  10.4025/actasciagron.v34i2.12616
Provedor de dados: 1
Autores: Moulin, Monique Moreira; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Rodrigues, Rosana; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Gonçalves, Leandro Simões Azeredo; Universidade Estadual do Norte Fluminense; Sudré, Cláudia Pombo; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Pereira, Messias Gonzaga; Universidade Estadual do Norte Fluminense Darcy Ribeiro.
Small farmers have played an important role in maintaining genetic resources of traditional varieties. Collecting and characterizing germplasm, especially landraces and heirloom varieties, are strategic activities that would create a knowledge base describing the traditional varieties of a given region and reduce the risk of genetic erosion. The objectives of this work were as follows: collect sweet potato roots from rural properties and local markets in Rio de Janeiro State, Brazil; characterize the accessions using RAPD and ISSR molecular markers, comparing the efficiency in discrimination of these two types of molecular markers; and estimate the genetic variability of the population. Fifty-nine samples from rural properties and 19 from local markets...
Palavras-chave: Genetic resources; Germplasm characterization; Genetic variability; Heirloom seeds.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12616

Cycle, canopy architecture and yield of common bean genotypes (Phaseolus vulgaris) in Santa Catarina State - doi: 10.4025/actasciagron.v35i1.15516
Provedor de dados: 1
Autores: Zilio, Marcio; Universidade do Estado de Santa Catarina; Souza, Clovis Arruda; Universidade do Estado de Santa Catarina; Coelho, Cileide Maria Medeiros; Universidade do Estado de Santa Catarina; Miquelluti, David Jose; Universidade do Estado de Santa Catarina; Michels, Adelina Ferreira; Universidade do Estado de Santa Catarina.
The objective of this study was to identify the phenotypic and genotypic correlations between the plant cycle and plant habit and the effect on yield in landrace bean genotypes. The experiment was conducted in Joaçaba and Lages, Santa Catarina State for the 2008/2009 crop using 26 bean genotypes: 22 landrace and 4 commercial genotypes obtained from the UDESC. We evaluated the number of days from emergence to flowering, number of days from flowering to physiological maturity and number of days from emergence to physiological maturity in relation to the genotypes’ cycle. The aerial plant architecture characteristics evaluated were the growth habit, plant habit, plant height, stem diameter, height of the first pod insertion and number of nodes on the main...
Tipo: Pesquisa científica de campo e laboratório
Palavras-chave: 5.01.03.00-8  Fitotecnia 5.01.03.01-6  Manejo e Tratos Culturais trail analysis; Genetic variability; Short cycle; Upright habit 5.01.03.00-8  Fitotecnia 5.01.03.01-6  Manejo e Tratos Culturais.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15516

Characterization of race 65 of Colletotrichum lindemuthianum by sequencing ITS regions
Provedor de dados: 1
Autores: Coelho, Marcela; Gonçalves-Vidigal, Maria Celeste; Sousa, Lorenna Lopes de; Nunes, Maria Paula Barion Alves; Azevedo, Rafhael Felipin; Galván, Marta Zulema.
The present work aimed characterize isolates of C. lindemuthianum race 65 from different regions in Brazil by ITS sequencing. A total of 17 isolates of race 65, collected in the states of Mato Grosso, Minas Gerais, Paraná, Santa Catarina and São Paulo, were studied. Analysis of the sequences of isolates 8, 9, 12, 14 and 15 revealed the presence of two single nucleotide polymorphisms (SNPs) in the ITS1 region at the same positions. These isolates, when analyzed together with the sequence of isolate 17, revealed a SNP in the ITS2 region. The highest genetic dissimilarity, observed between isolates 11 and  3 and between isolates 11 and 10, was 0.772. In turn, isolates 7 and 2 were the most similar, with a value of 0.002 for genetic distance. The phylogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.01.00.00-9; 5.01.03.00-8 anthracnose; Genetic variability; Pathogens; Dissimilarity Melhoramento Vegetal.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30586

Agrupamento de acessos e cultivares de três espécies de Brachiaria por RAPD - DOI: 10.4025/actasciagron.v30i4.5298
Provedor de dados: 1
Autores: Ambiel, Ana Claudia; UNOESTE; Guaberto, Luciana Machado; UNOESTE; Vanderlei, Talita Marques; UNOESTE; Machado Neto, Nelson Barbosa; UNOESTE.
O propósito deste trabalho foi determinar a similaridade genética entre acessos de germoplasma e cultivares comerciais de três espécies de Brachiaria (inter e intraespecífica), por meio de marcadores RAPD. Sementes foram utilizadas como fonte de DNA. 10 “primers” decâmeros foram selecionados de 120 “primers” avaliados, produzindo 107 bandas polimórficas, as quais foram utilizadas para a análise de variância molecular (Amova), o coeficiente de similaridade de Jaccard e índices de fixação gênica. 24,40% da variabilidade genética total estão contidas entre as espécies e 75,60% dentro destas, com índice de fixação gênica (FST) 0,24. No dendrograma, houve a formação de dois ramos, um formado por P. maximum e B. arrecta e o outro subdividido em três: todas...
Palavras-chave: 5.02.01.03-4    Genética e Melhoramento Florestal Brachiaria; Marcadores moleculares; Variabilidade genética; Dendrograma; Índice de fixação gênica. Brachiaria; Molecular markers; Genetic variability; Dendrogram; Genetic fixation index.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5298

Higher-precision experimental statistics for the selection of early and upright common bean lines
Provedor de dados: 1
Autores: Ribeiro, Nerinéia Dalfollo; Steckling, Skarlet De Marco; Maziero, Sandra Maria; Klasener, Greice Rosana.
The development of early and upright common bean cultivars is necessary to meet the demand of producers. The objectives of this work were to evaluate more precise experimental statistics for the selection of early and upright common bean lines and identify the traits that provide better genetic differentiation between lines. For this purpose, 156 common bean lines of different grain colours were evaluated in 23 experiments carried out in the southern region of Brazil between 1998 and 2015. The traits related to earliness (flowering and cycle) and upright plant architecture (lodging, insertion of the first pod and insertion of the last pod) were evaluated with high experimental precision by determining F-test values for genotype (Fc), heritability (h2), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Genetic variability; Statistical parameters; Selection efficiency.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42725

Morphophysiological characterization in artichoke accessions aimed at selecting materials for in natura consumption - doi: 10.4025/actasciagron.v34i4.14551
Provedor de dados: 1
Autores: Costa, Angélica Reolon da; Universidade de Passo Fundo; Grando, Magali Ferrari; Universidade de Passo Fundo.; Scheffer-Basso, Simone Meredith; Universidade de Passo Fundo; Cravero, Vanina Pamela; Universidade Nacional de Rosário.
A morphophysiological characterization of 19 accessions within an artichoke germplasm collection was conducted with the aim of determining genetic variability and to select materials suitable for in natura consumption to be used in genetic breeding. The collection was composed of commercial accessions, materials collected in South Brazil, and of foreign origin. Twenty quantitative, five multicategorical and two binary traits were evaluated. Quantitative data were submitted to a multivariate analysis. Genetic variability was observed for almost all characteristics. The traits with the greatest relative contribution to genetic divergence, which accounted for 79.54% of the variability, were as follows: the thickness and diameter bottom, bottom fresh mass/head...
Tipo: Pesquisa Empírica de Campo
Palavras-chave: 5.01.00.00-9  Agronomia; 5.01.03.05-9  Melhoramento Vegetal Cynara cardunculus var. scolymus (L.) Fiori; Genetic variability; Germplasm; Multivariate analysis Melhoramento Vegetal.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/14551

Genetic monitoring by rapd markers for repopulation programs of Salminus brasiliensis (Pisces, Characiformes) - doi: 10.4025/actascianimsci.v35i2.15904
Provedor de dados: 2
Autores: Almeida, Fernanda Simões de; Universidade Estadual de Londrina; Lopes, Carla Martins; Universidade Federal do Rio Grande do Sul; Orsi, Mário Luís; Universidade Estadual de Londrina; Sirol, Rodolfo Nardez; Companhia Paulista de Força e Luz; Sodré, Leda Maria Koelblinger; Universidade Estadual de Londrina.
The preservation of the genetic variability of hatchery stocks used to supplement natural populations is a priority. Current study employed RAPD markers to examine the genetic diversity of dourado samples from the middle Paranapanema river and from the broodstock used in the stock enhancement program of the Aquaculture and Hydrobiology Station at the Salto Grande Hydroelectric Power Plant. Nineteen RAPD primers were analyzed, which generated 299 bands and the latter were used for genetic analysis. The percentage of polymorphic fragments was higher in stock captured in the Paranapanema river than from fry stocks. The genetic diversity was lower in the broodstock than in natural population. Amova results showed that most inter-population genetic variation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.06.03.00-0 ou 2.02.04.00-0 dourado; Genetic variability; Hatchery stocks; Fry stocks Dourado; Genetic variability; Hatchery stocks; Fry stocks.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/15904

Diversidade genética de três estoques de piapara (Leporinus elongatus), utilizando RAPD - DOI: 10.4025/actascianimsci.v30i2.4710
Provedor de dados: 2
Autores: Gomes, Patricia Cristina; UEM; Ribeiro, Ricardo Pereira; UEM; Barrero, Nelson Lopera; UEM; Povh, Jayme Aparecido; UEM; Vargas, Lauro; UEM; Sirol, Rodolfo Nardez; Duke Energy International Geração Paranapanema.
Recentemente a produção aquícola brasileira tem apresentado grande progresso. Dentre as espécies nativas cultivadas no Brasil, a piapara (Leporinus elongatus) tem sido amplamente preconizada. Com objetivo de avaliar os programas de repovoamento, foram analisadas a variabilidade e a divergência genética de três estoques de piapara com a técnica de RAPD (Random Amplified Polymorphic). O primeiro estoque pertence à Estação de Aquicultura e Hidrologia da Duke Energy International (A); o segundo, à piscicultura de Rolândia (B) e o terceiro, ao Programa de Repovoamento dos Rios do Paraná (C). Os dez primers para RAPD utilizados produziram 105 fragmentos polimórficos, conferindo um polimorfismo de 98,1% para os três estoques avaliados. A porcentagem de locos...
Palavras-chave: 5.05.04.00-2    Reprodução Animal divergência genética; Locos polimórficos; Peixe; RAPD; Reprodutores; Variabilidade genética. genetic divergence; Polymorphic loci; Fish; RAPD; Reproducers; Genetic variability.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4710

Avaliação da variabilidade das gerações G0 e F1 da linhagem GIFT de tilápia do Nilo (Oreochromis niloticus) por RAPD - DOI: 10.4025/actascianimsci.v30i2.4708
Provedor de dados: 2
Autores: Lupchinski Junior, Enio; UEM; Vargas, Lauro; UEM; Povh, Jayme Aparecido; UEM; Ribeiro, Ricardo Pereira; UEM; Mangolin, Claudete Aparecida; UEM; Barrero, Nelson Maurício Lopera; UEM.
Este trabalho teve como objetivo analisar, pela técnica RAPD, a variabilidade e a divergência genética de duas gerações da linhagem GIFT. Foram estimados parâmetros para os reprodutores (G0) e para a progênie (F1). A variabilidade genética foi determinada pela porcentagem de loci polimórficos e pelo índice de Shannon. As gerações apresentaram 69,6% de loci polimórficos (G0) e 60,0% de polimorfismo (F1). Os valores para o índice de Shannon foram de 0,367 para a geração G0 e de 0,317 para a F1. Os valores de divergência genética, calculados pelo teste de Mantel, foram de 0,213 para a G0 e 0,208 para a geração F1. Os resultados obtidos indicaram que houve perda da variabilidade genética da geração G0 para a F1. No entanto, um fato a ser destacado foi a alta...
Palavras-chave: 5.05.04.00-2    Reprodução Animal índice de Shannon; Gerações de cultivo; Divergência genética; Variabilidade genética. Shannon index; Cultivated generations; Genetic divergence; Genetic variability.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4708

Genetic diversity of Lippia rotundifolia Cham. in Minas Gerais, Brazil
Provedor de dados: 3
Autores: Meira, Messulan Rodrigues; Martins, Ernane Ronie; Resende, Luciane Vilela.
Lippia rotundifolia is an aromatic species, endemic from Campos rupestres and isolated by mountains. We aimed with the research to study the genetic variability of chá-de-pedestre (Lippia rotundifolia Cham.) with natural occurrence in ten places of the State of Minas Gerais, from molecular markers of ISSR type. The places of collection were: Parque Estadual de Serra Nova; Parque Estadual Veredas do Peruaçu; Abóboras community; Gigante community; edge of Rio do Peixe; environmental preservation area of Olhos d´água; private property in Joaquim Felício; Parque Estadual do Rio Preto; São Gonçalo do Rio das Pedras and brook of Rio Tigre. After the establishment of the extraction and amplification of DNA fragments with primers ISSR, an array of genetic distance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.02.03.00-4 pedestrian tea; Genetic variability; Medicinal plant; Conservation Genética Vegetal.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42754

Allozyme analysis of the four species of Hypostomus (Teleostei: Loricariidae) from the Ivaí river, upper Paraná river basin, Brazil - doi: 10.4025/actascibiolsci.v35i4.16355
Provedor de dados: 3
Autores: Paiva, Suzana de; Universidade Estadual de Maringá; Zawadzki, Claudio Henrique; Universidade Estadual de Maringa; Ruvulo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá; Lapenta, Ana Silvia; Universidade Estadual de Maringá; Renesto, Erasmo; Universidade Estadual de Maringá.
Allozyme electrophoresis analysis were performed in four species of Hypostomus (Loricariidae), H. albopunctatus, H. hermanni, H. regani, e Hypostomus sp. 1/NUP 5612 from the Ivaí river, a tributary of the upper Paraná river. The study of 14 loci revealed diagnostic characters and exclusive alleles in a low frequency. The heterozygosity ranged from 0.000 in H. albopunctatus to 0.199  in H. hermanni, which was higher than the heterozygosity in other samples of Hypostomus in literature, as well as in other fish groups. Hypostomus albopunctatus and H. regani revealed higher similarity (I = 0.804), while H. hermanni and Hypostomus sp. 1/NUP 5612 showed the least genetic identity (I = 0.569). All samples were genetically distinguished, despite there were several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.02.00.00-5 catfish; Heterozygosity; Neotropical fishes; Genetic variability; Siluriformes Genética.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/16355

Genetic variation in native and farmed populations of Tambaqui (Colossoma macropomum) in the Brazilian Amazon: regional discrepancies in farming systems
Provedor de dados: 42
Autores: Aguiar,Jonas; Schneider,Horacio; Gomes,Fátima; Carneiro,Jeferson; Santos,Simôni; Rodrigues,Luis R.; Sampaio,Iracilda.
The tambaqui, Colossoma macropomum, is the most popular fish species used for aquaculture in Brazil but there is no study comparing genetic variation among native and farmed populations of this species. In the present study, we analyzed DNA sequences of the mitochondrial DNA to evaluate the genetic diversity among two wild populations, a fry-producing breeding stock, and a sample of fish farm stocks, all from the region of Santarém, in the west of the Brazilian state of Pará. Similar levels of genetic diversity were found in all the samples and surprisingly the breeding stock showed expressive representation of the genetic diversity registered on wild populations. These results contrast considerably with those of the previous study of farmed stocks in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tambaqui; Mitochondrial DNA; Genetic variability; Aquaculture.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401439

Genetic diversity assessed by microsatellite markers in the amphicarpic species Trifolium polymorphum Poir.
Provedor de dados: 42
Autores: CONTERATO,IONARA F.; SCHIFINO-WITTMANN,MARIA TERESA; GUERRA,DIVANILDE; BÜTTOW,MIRIAM V.; AGNOL,MIGUEL DALL’; DAHMER,NAIR.
ABSTRACT Trifolium polymorphum Poir. is an amphicarpic forage legume from southern Brazil, Uruguay, Argentina, Paraguay and Chile. Information on the genetic diversity of natural populations in natural grasslands in southern Brazil is limited. In order to increase the knowledge about this species, an analysis of the genetic diversity was carried out in 10 natural populations of T. polymorphum with the use of 20 microsatellite markers. The expected heterozygosity in T. polymorphum populations ranged from 0.40 to 0.43, with a mean of 0.42. A total of 193 alleles were detected with a mean of 9.3 alleles per locus and polymorphic information content (PIC) for these markers of 0.62 to 0.89 with a mean of 0.84.The grouping based on the Jaccard’s coefficient of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Microsatellites; Natural grasslands; Pampa biome.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401685

Molecular characterization of the invasive aquatic macrophyte Hydrilla verticillata (Hydrocharitaceae) in Brazil
Provedor de dados: 42
Autores: LUCIO,LÉIA CAROLINA; THOMAZ,SIDINEI M.; PRIOLI,SÔNIA MARIA A.P.; BONI,TALGE A.; OLIVEIRA,ALESSANDRA V. DE; PRIOLI,ALBERTO JOSÉ.
Abstract: Invasive populations of macrophytes are widely distributed and have been successfully introduced and established in freshwater habitats. Hydrilla verticillata was first recorded in 2005 in the Upper Paraná River floodplain and in 2007 at the Itaipu Reservoir (Brazil-Paraguay border, ca. 300 km downstream from its first record). However, its genetic variability within different sites in South America is unknown. We used nucleotide sequences corresponding to the trnL-trnF fragment cpDNA to genetically characterize populations of H. verticillata in different ecosystems of the Upper Paraná River basin. The results indicated an absence of genetic differentiation within and between populations of the basin, and even individuals collected 600 km apart...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CpDNA; Genetic variability; TrnL-trnF; Upper Paraná River basin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500623

RAPD analysis revealing polymorphism in egg parasitoids of soybean stink bugs (Hemiptera: Pentatomidae)
Provedor de dados: 44
Autores: Aljanabi,Salah M.; Loiácono,Marta S.; Lourenço,Rodrigo T.; Borges,Miguel; Tigano,Myrian S.
The solitary egg parasitoids (Hymenoptera: Scelionidae) of Pentatomidae species complex are potential candidates for soybean (Glycine max L.) integrated pest management programs in Brazil. The correct identification of species and strains of these parasitoids is necessary to implement the use of biological control. Polymerase chain reaction (PCR) was used to generate random amplification of polymorphic DNA (RAPD) from male individuals of Telenomus podisi Ashmead and Trissolcus spp. collected in a soybean field in Brasília, Brazil in 1996. The analysis of 18 primers used to screen Trissolcus spp. individuals revealed different patterns related to the species analyzed. These results indicate the potential of RAPD markers to differentiate T. teretis Johnson,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Microhymenoptera parasitoids; Genetic variability; Molecular markers; RAPD.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000300009

Polymorphism of the grasshopper Schistocerca pallens (Thunberg) (Orthoptera: Acrididae) and its natural pathogen Metarhizium flavoviride Gams &amp;amp; Rozsypal (Hyphomycetes), revealed by RAPD analysis
Provedor de dados: 44
Autores: Silveira,Eni B.; Al-Janabi,Salah M.; Magalhães,Bonifácio P.; Carvalho,Luiz J.C.B.; Tigano,Myrian S.
The genetic variability of 27 individuals from two distinct populations (Northeast and Central Brazil) of the grasshopper Schistocerca pallens (Thunberg) and eight isolates of its natural pathogen, the fungus Metarhizium flavoviride Gams &amp;amp; Rozsypal, was investigated using RAPD analysis. For the grasshoppers, ten different 10-mer oligonucleotide primers of arbitrary sequence were selected for analysis, resulting in 79 scorable binary characters. The program RAPDDIP applied to S. pallens revealed nucleotide diversity of 2.3 and 2.2% for the populations from Rio Grande do Norte (Northeast Brazil) and Federal District (Central Brazil), respectively. These values indicate the presence of high genetic variability within these populations. Conversely, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Genetic variability; Entomopathogenic fungus; Biological control; Molecular markers.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000100012

Utilization of microsatellite markers to form families of "pejerrey" Odontesthes bonariensis in a genetic breeding program
Provedor de dados: 45
Autores: Tavares,R.A; Nunes,M.D; Almeida,D.B; Silva,J.C; Vaz,B.S; Moreira,C.G.A; Dionello,N.J.L; Piedras,S.R.N; Moreira,H.L.M.
Foram identificadas a divergência e a variabilidade genética, por meio do polimorfismo de seis marcadores microssatélites, de duas populações de Odontesthes bonariensis, utilizadas em manejos de cultivo e com potencial para fornecimento de reprodutores para programas de melhoramento genético. Do total de seis loci, cinco demonstraram eficiência para análise genética nas duas populações de O. bonariensis. A diferenciação genética significante nas populações analisadas pode fornecer a base para futuros programas de melhoramentos genéticos, através da combinação de material das populações divergentes para o desenvolvimento de linhagens ou execução de um programa de seleção.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Genetic variability; Polymorphism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500034

Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis
Provedor de dados: 48
Autores: SANT’ANNA,JULIANE R; MIYAMOTO,CLÁUDIA T; ROSADA,LÚCIA J; FRANCO,CLAUDINÉIA C S; KANESHIMA,EDILSON N; CASTRO-PRADO,MARIALBA A A.
The genetic variation among nine soybean-originating isolates of Colletotrichum truncatum from different Brazilian states was studied. Nitrate non-utilizing (nit) mutants were obtained with potassium chlorate and used to characterize vegetative compatibility reactions, heterokaryosis and RAPD profile. Based on pairings of nit mutants from the different isolates, five vegetative complementation groups (VCG) were identified, and barriers to the formation of heterokaryons were observed among isolates derived from the same geographic area. No complementation was observed among any of the nit mutants recovered from the isolate A, which was designed heterokaryon-self-incompatible. Based on RAPD analysis, a polymorphism was detected among the wild isolate C and...
Tipo: Journal article
Palavras-chave: Genetic variability; Heterokaryosis; Parasexual recombination; RAPD.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100007

Genetic diversity in Mexican wild populations of the Great Curassow (Crax rubra)
Provedor de dados: 49
Autores: Morales-Contreras,Jonathan; Escalante,Patricia; Matías-Ferrer,Noemí.
Abstract The Great Curassow (Crax rubra) is a Neotropical bird with a wide distribution; it is classified under different threat categories and is listed as a vulnerable species by the IUCN. The Official Mexican Standard, the NOM-059-SEMARNAT-2010, indicates that the Great Curassow is a threatened species, and the subspecies Crax rubra griscomi, which is restricted to the island of Cozumel, is classified as critically endangered. Habitat loss and fragmentation, hunting, overexploitation, and illegal trade are among the main factors that have placed the bird at an endangered status. The objective of the present study was to determine the genetic structure and variation of the species within the Mexican populations of Crax rubra by using three mitochondrial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cracids; Noninvasive collection; Genetic variability; Mitochondrial genes; Nuclear gene; Gene pool; Conservation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032019000100209

Morphological and AFLP markers for describing genetic relationships among white-oat genotypes
Provedor de dados: 53
Autores: Benin,Giovani; Carvalho,Fernando Irajá Félix de; Oliveira,Antonio Costa de; Marchioro,Volmir Sérgio; Vieira,Eduardo Alano; Bertan,Ivandro; Valério,Igor Pires; Marchese,José Abramo; Matei,Gilvani.
The use of different genetic dissimilarity indicators can help in both the selection of crop improvement strategies and as artificial crosses. The objectives of this work were to estimate the genetic dissimilarity among seven oat genotypes (Avena sativa L.) through molecular (dg) and morphological (dm) markers. Data were obtained from two experiments carried out in Capão do Leão County, RS, Brazil, in 2000. The estimated correlation coefficient (r = 0.33) indicated a low association between dg and dm estimates. The genotypes with similar pedigrees (UPF 16 and UPF 17), with a bootstrapping value of 82.7%, performed the most consistent group (dg) and clustered more closely in both techniques (dg and dm). However, genotypes with similar pedigree clustered in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Choice of parents; Genetic dissimilarity; Genetic variability.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052008000300002

Fusarium verticillioides strains isolated from corn feed: characterization by fumonisin production and RAPD fingerprinting
Provedor de dados: 52
Autores: Ono,Elisabete Yurie Sataque; Fungaro,Maria Helena Pelegrinelli; Sofia,Silvia Helena; Miguel,Tatiana de Ávila; Sugiura,Yoshitsugu; Hirooka,Elisa Yoko.
In this study a total of 16 Fusarium verticillioides strains isolated from corn feed samples were characterized by fumonisin (FB) production and random amplified polymorphic DNA (RAPD). All the strains produced FB1 and FB2 with levels ranging from 2.41 to 3996.36 µg/g, and from 1.18 to 1209.91 µg/g, respectively. From the 16 F. verticillioides strains, four were identified as low (3.59 to 1289.84 µg/g), eight as intermediate (&gt;1289.84 to 3772.44 µg/g) and four strains as high (&gt;3772.44 µg/g) fumonisin producers. From the total of 105 loci amplified, 60 (57.14%) were polymorphic. RAPD analysis showed very similar patterns among low, moderate and high fumonisin-producing strains. Although RAPD markers were capable of discriminating the different F....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fumonisin; Fusarium verticillioides; Genetic variability; RAPD.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400026

Random amplified polymorphisms between two South American subspecies of rattlesnakes (Crotalus durissus collilineatus and Crotalus durissus terrificus)
Provedor de dados: 52
Autores: Echeverrigaray,Sergio; Grazziotin,Gilberto; Grazziotin,Felipe; Agostini,Gustavo.
The present work was made to determine the suitability of RAPD analysis for the identification of specimens of two subspecies of South American rattlesnakes (Crotalus durissus terrificus and Crotalus durissus collilineatus). The 11 arbitrary primer sequence tested amplified a total of 161 bands, of which 31% were polymorphics when compared with Crotalus specimens. The combining results from different primers allowed the identification of all the specimens under analysis. Several characteristic bands of each subspecies were identified. Dendrogram, based on RAPD markers, show three groups that corresponded to the subspecies of Crotalus, and to Bothrops jararaca specimen.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rattlesnake; RAPD; Genetic variability; Molecular markers.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300014

Phenetic analysis of Panstrongylus megistus Burmeister, 1835 (Hemiptera: Reduviidae: Triatominae) in the State of Paraná-Brazil
Provedor de dados: 52
Autores: Kopp,Rogério Luiz; Thomaz-Soccol,Vanete; Klisiowicz,Débora do Rocio; Membrive,Norberto; Barata,José Maria Soares; Jurberg,José; Steindel,Mário; Kopp,Denize Cristina Trevisan; Castro,Edilene Alcântara de; Luz,Ennio.
Panstrongylus megistus is an important Chagas Disease vector and is said to be one of the species that might replace Triatoma infestans as the main vector of that disease in Brazil. The different degrees of P. megistus domiciliation in Brazil and its epidemiological relevance draw forth the need for the development of genetic studies that make it possible to analyze and understand the interchange of individual and gene fluxes among different populations. Thus, the present work aimed at studying the genetic variability of P. megistus in the State of Paraná - south of Brazil- and at comparing it with populations of the same species from five other states in Brazil (SP, MG, SC, RS, SE). In order to attain the proposed objective, 25 populations were studied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panstrongylus megistus; Isoenzymes; Genetic variability; Triatomines.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200012

Genetic variability in regenerated Metarhizium flavoviride protoplasts
Provedor de dados: 52
Autores: Kuklinsky-Sobral,Júlia; Luna-Alves-Lima,Elza Áurea de; Araújo,Janete Magali de; Azevedo,João Lúcio.
Protoplast isolation and regeneration were evaluated in two wild-type and two colour mutant strains of Metarhizium flavoviride. Cultivation in liquid medium, followed by mycelium treatment with Novozym 234 in the presence of KCl 0.7M as osmotic stabilizer, produced 5.05 x 10(6) to 1.15 x 10(7)x mL-1 protoplasts. The percentage of regeneration ranged from 6.65 to 27.92%. Following protoplast regeneration, one strain produced spontaneously stable morphological variant colonies. Although colonies with altered morphology have been reported in bacteria following protoplast regeneration, this is the first time that the same is described in a filamentous fungus. The original strain and one derived variant were tested for sensitivity to the fungicides benomyl and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metarhizium flavoviride; Protoplasts; Genetic variability; Benomyl; Captan.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100001

Genetic variability of pre and post-fragmentation cohorts of Aspidosperma polyneuron Muell. Arg. (Apocynaceae)
Provedor de dados: 52
Autores: Torezan,José Marcelo Domingues; Souza,Rogério Fernandes de; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Camargo,Elvis Hassmann; Vanzela,André Luís Laforga.
RAPD was used to access the genetic variability in Aspisdosperma polyneuron, a long-lived, late-reproducing tropical tree, and highly important for the Atlantic Forest. RAPD profiles from adults (pre-fragmentation, &gt;300 years old) and seedlings (post-fragmentation, &lt;&lt;50 years old) were analyzed. Results showed a decrease of genetic polymorphism of post-fragmentation cohorts in small fragments and higher genetic diversity within population. The genetic diversity distribution suggested the establishment of fragments as protected reserves, and the transference of seedlings among fragments for conservation of A. polyneuron.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspidosperma; DNA markers; Forest fragmentation; Genetic variability; Peroba-rosa; RAPD.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200002

Microsatellite analysis of the parental contribution of Piaractus mesopotamicus to the production of offspring in the semi-natural system of reproduction
Provedor de dados: 52
Autores: Povh,Jayme Aparecido; Ribeiro,Ricardo Pereira; Sirol,Rodolfo Nadez; Streit Jr.,Danilo Pedro; Moreira,Heden Luiz Marques; Siewerdt,Frank; Lopera-Barrero,Nelson Mauricio; Mangolin,Claudete Aparecida; Vargas,Lauro.
The objective of this study was to evaluate the genetic diversity and the parental contribution of Piaractus mesopotamicus in the production of offspring in the semi-natural system of reproduction. Twenty parental fishes (eleven males and nine females) and the total of 100 larvae were evaluated by microsatellite marker. The parents and offspring had thirty-one alleles and heterozygosity of 0.550 and 0.563, respectively. The females were fertilised by two up to six males while the males fertilised three up to five females. The contribution of the females and males to the offspring were 66.6 and 58%, respectively. Such results indicated no loss in the genetic variability in the offspring, and the parents had multiple paternity and reasonable contribution to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Stock enhancement programme; Reproductive management.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200018

Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion
Provedor de dados: 52
Autores: Diniz,Leandro Eugênio Cardamoni; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Torres,Fabrício Medeiros; Ruas,Eduardo Augusto; Santos,Melissa de Oliveira; Sera,Tumoru; Ruas,Paulo Maurício.
The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Genetic variability; RAPD; Restriction endonucleases.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500002

Population Structure and Genetic Diversity of Sweet Cassava Accessions from the Midwestern, Southeastern and Southern Regions of Brazil
Provedor de dados: 52
Autores: Ortiz,Alex Henrique Tiene; Vidigal Filho,Pedro Soares; Rocha,Vanesca Priscila Camargo; Ferreira,Rebecca Caroline Ulbricht; Gonçalves,Tiago Maretti; Gonçalves-Vidigal,Maria Celeste.
Abstract The objective of this work was to screen sweet cassava accessions collected in smallholding areas in the Midwestern, Southeastern and Southern regions of Brazil, using 15 SSR molecular markers, to determine population structure and genetic diversity. Polymorphism was detected in every loci analyzed, with mean of 6.33 alleles per locus, and mean polymorphism information content (PIC) of 0.6057, pointing out that the primers were highly informative. The observed heterozygosity ranged from 0.0709 (SSRY 101) to 0.9398 (GA 12), with a mean of 0.6511, and mean genetic diversity of 0.6578, ranging from 0.3592 (GA 136) to 0.8116 (SSRY 21). The most dissimilar combinations observed were BGM526PR-BGM596MS, BGM526PR-BGM622MS and BGM526PR-BGM629MS. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Germplasm characterization; Genetic variability; SSR markers.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100206

Preliminary analysis of the genetic variability of two natural beds of the Scallop Euvola ziczac (Linnaeus, 1758) in Brazil
Provedor de dados: 52
Autores: Wangüemert,Mercedes Gonzalez; Pezzuto,Paulo Ricardo; Borzone,Carlos Alberto.
Euvola ziczac (formerly Pecten ziczac), a simultaneous hermaphroditic scallop was heavily fished in Brazil between 1972 and 1980. The production peaked in 1980 with 8,800 tons and was followed by the total collapse of the resource. In order to investigate the possible loss of genetic variability of the stock associated to overfishing and self-fertilization, the polymorphism of phosphoglucomutase (PGM) and glucose phosphate isomerase (GPI) was analyzed by electrophoresis of the adductor muscle of scallops from São Francisco (26° 20.583’S; 48° 16.507’W) and Bom Abrigo (25° 28.735’S; 47° 37.621’W) beds; the southern and northern extremes of the scallop fishing ground, respectively. Animals from São Francisco showed a strong deficiency of heterozygosity for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Stock depletion; Scallop; Euvolaziczac; Brazil.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200014

Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800)
Provedor de dados: 56
Autores: Artico,L.O.; Bianchini,A.; Grubel,K.S.; Monteiro,D.S.; Estima,S.C.; de Oliveira,L.R.; Bonatto,S.L.; Marins,L.F.
The South American sea lion, Otaria flavescens, is widely distributed along the Pacific and Atlantic coasts of South America. However, along the Brazilian coast, there are only two nonbreeding sites for the species (Refúgio de Vida Silvestre da Ilha dos Lobos and Refúgio de Vida Silvestre do Molhe Leste da Barra do Rio Grande), both in Southern Brazil. In this region, the species is continuously under the effect of anthropic activities, mainly those related to environmental contamination with organic and inorganic chemicals and fishery interactions. This paper reports, for the first time, the genetic diversity of O. flavescens found along the Southern Brazilian coast. A 287-bp fragment of the mitochondrial DNA control region (D-loop) was analyzed. Seven...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Otaria flavescens; MtDNA; D-loop; Genetic variability; New haplotypes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900002

Genetic variability of Brazilian isolates of Alternaria alternata detected by AFLP and RAPD techniques
Provedor de dados: 58
Autores: Dini-Andreote,Francisco; Pietrobon,Vivian Cristina; Andreote,Fernando Dini; Romão,Aline Silva; Spósito,Marcel Bellato; Araújo,Welington Luiz.
The Alternaria brown spot (ABS) is a disease caused in tangerine plants and its hybrids by the fungus Alternaria alternata f. sp. citri which has been found in Brazil since 2001. Due to the recent occurrence in Brazilian orchards, the epidemiology and genetic variability of this pathogen is still an issue to be addressed. Here it is presented a survey about the genetic variability of this fungus by the characterization of twenty four pathogenic isolates of A. alternata f. sp. citri from citrus plants and four endophytic isolates from mango (one Alternaria tenuissima and three Alternaria arborescens). The application of two molecular markers Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) had revealed the isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternaria brown spot; Molecular markers; Citrus plants; PCA analysis; Genetic variability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300032

Genetic variability of Streptococcus mutans isolated from low-income families, as shown by RAPD markers
Provedor de dados: 58
Autores: Moreira,Mônica; Vicente,Vânia Aparecida; Glienke,Chirlei.
The detection of Streptococcus mutans isolates with high genetic variability in different individuals indicates the occurrence of transmissibility. In this context, nine low-income families (40 individuals in total) with similar social conditions were assessed to identify the bioserotypes of S. mutans using both biochemical and RAPD markers and to establish the degree of similarity among the intra-familial isolates. The polymorphism analysis used the coefficient of Jaccard in both a Principal Coordinates Analysis (PCO) and in a UPGMA clustering method. A total of 157 isolates were obtained from salivary samples, using the morphology of colonies recovered using MSB agar as indicators. From those, 64 were characterized biochemically as S. mutans and 10 as S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus mutans; Genetic variability; RAPD.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400026

Genetic Parameters and Predictive Genetic Gain in Maize with Modified Recurrent Selection Method
Provedor de dados: 117
Autores: Afonso Doná,Anderson; Vieira Miranda,Glauco; Oliveira DeLima,Rodrigo; Gonçalves Chaves,Luciana; Gomes e Gama,Elto Eugênio.
The modified recurrent selection method suggests a less productive population to test the other population more productive and itself aiming to have more genetic gains than tradition recurrent selection method that uses one population to test the other one. The objectives of this work were to estimate the genetic components, to predict and to evaluate the genetic gains of the SynFlint and Syndent populations of maize (Zea mays L.), applying a modified recurrent selection method. Two recombination cycles were carried out, with 144 Si progeny per cycle. These progenies were test crossed with SynFlint, generating a SynFlint x Syndent inter-population and a SynFlint x SynFlint intra-population. The progenies were evaluated in five environments in the first...
Tipo: Journal article
Palavras-chave: Breeding methods; Genetic variability.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000100006

Diversity of Genetic Resources and Genetic Association Analyses of Green and Dry Chillies of Eastern India
Provedor de dados: 117
Autores: Chattopadhyay,Arup; Baran Sharangi,Amit; Dai,Nuka; Dutta,Subrata.
Chilli (Capsicum annuum L.) is regarded as one of the main commercial vegetable and spice crops at the global level. Maximum diversity can be noted among the cultivars/landraces available in India with respect to shape, size, yield, quality, and other traits. The present experiment was conducted to identify the most promising chilli variety suited for green and dry purposes, to study the genetic variability for different traits and to assess the association of different yield attributing traits with the green and dry yield of chilli. Thirty four genotypes were characterized during a 2-yr period. Most of the genotypes possessed the character constellation of C. annuum. Two genotypes, ‘Chaitali Pointed’ and ‘BC CH Sel-4’ were found most promising with...
Tipo: Journal article
Palavras-chave: Quantitative traits; Qualitative traits; Genetic variability; Heritability; Selection index.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300002

Genetic variation within three populations of Phycella australis (Phil.) Ravenna from Biobío Region, Chile, evaluated using ISSR markers
Provedor de dados: 117
Autores: Flores,Cristian; Delaveau,Catherine; Alvarez,Carolina; Vergara,Dafne; Baeza,Carlos; Ruiz,Eduardo; Uribe,Matilde.
Phycella australis (Phil.) Ravenna is a Chilean plant with high ornamental potential; however, the intensive extraction as a cut flower might be detrimental for the conservational state by ignoring the state of genetic variation. The objective of this investigation was to assess genetic variability within and between three populations of P. australis in the Biobío Region using inter-simple sequence repeat (ISSR) markers. The evaluated areas correspond to three locations in the province of Concepción, Biobío Region: Desembocadura (36°48' S, 73°10' W), Santa Juana (36°58' S, 72°58' W), and Lipinhue (37°00' S, 72°58' W). Six ISSR primers were used obtaining 51 fragments, from which 72.5% were polymorphic. From the three evaluated sites Santa Juana showed a...
Tipo: Journal article
Palavras-chave: Amaryllidaceae; Genetic variability; Molecular marker; Ornamental plant.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000100012

Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers
Provedor de dados: 118
Autores: Alem,Diego; Narancio,Rafael; Díaz Dellavalle,Paola; Rebuffo,Mónica; Zarza,Rodrigo; Dalla Rizza,Marco.
Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite...
Tipo: Journal article
Palavras-chave: Genetic variability; Lotus japonicus; SSR.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000300015

Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
Provedor de dados: 65
Autores: Busso,Cleverson; Kaneshima,Edilson Nobuyoshi; Franco,Francisco de Assis; Querol,Carmen Boto; Castro-Prado,Marialba Avezum Alves de.
Fusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusarium graminearum; Genetic variability; RAPD; Vegetative compatibility groups.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050

Molecular variants of Grapevine rupestris stem pitting-associated virus infecting grapevines (Vitis spp.) in Brazil
Provedor de dados: 65
Autores: Fajardo,Thor Vinícius Martins; Kin,Aléxis Cardama; Nickel,Osmar.
ABSTRACT: Grapevine rupestris stem pitting-associated virus (GRSPaV) is one of the most common viruses of grapevine. It is involved in the graft-transmissible disease rupestris stem pitting of the rugose wood complex. The objective of the research was to perform the molecular characterization of the coat protein (CP) gene of sixteen Brazilian GRSPaV isolates aiming to determine the occurrence of molecular variants (strains) of this virus. Nine grapevine samples were evaluated, from which dsRNA was extracted. Nucleotide sequences were generated by Next generation sequencing (NGS). Fifteen complete sequences of the GRSPaV CP gene were obtained and phylogenetically analyzed. Multiple alignments of the sequences showed identities of nucleotides ranging from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GRSPaV; Foveavirus; Strains; Lineages; Genetic variability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100153

Genetic divergence among soybean cultivars in irrigated lowland in the State of Tocantins
Provedor de dados: 65
Autores: Peluzio,Joênes Mucci; Pires,Luiz Paulo Miranda; Cancellier,Leandro Lopes; Afférri,Flávio Sérgio; Colombo,Gustavo André; Teixeira Júnior,Thadeu; Ribeiro,Glauber Ronery dos Santos.
In order to evaluate the genetic divergence among soybean cultivars, an assay was carried out at Formoso do Araguaia, TO, Brazil, in off-season of 2007, in irrigated lowland conditions. The experimental design were randomized blocks with 12 treatments and three replications. Genetic divergence was evaluated by multivariate procedures: Mahalanobis distance, Tocher clustering method's and nearest neighbor method. It was observed the formation of two distinct groups by the dendrogram of genetic dissimilarity, which were identical to those groups formed by Tocher's method. The Tocher optimization method and the nearest neighbor agreed among themselves. The traits number of days to maturity, plant height and weight of 100 seed were the ones that most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Genetic variability; Hybrid combination.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000300002

Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
Provedor de dados: 65
Autores: Freitas,Loreta Brandão de; Koehler-Santos,Patrícia; Salzano,Francisco Mauro.
Leaves from 14 Brazilian genotypes of Triticum aestivum L. were treated with salicylic acid to induce pathogenesis-related (PR) proteins. Inter and intracellular extracts were then obtained and investigated through polyacrilamide gel electrophoresis. Seven bands were observed. Material related to two of them (of 40 and 24 kDa) occurred in intracellular spaces only. DNA from these same genotypes was then amplified through PCR using primers developed from three sequences encoding PR proteins, and compared with previously described sequences. The fragments presented homologies to PR groups 1, 3 (chitinases), and 5 (thaumatin-like). The PR3-like sequence also showed a site characteristic of PRs induced by ethylene and a portion without homology with previous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum L.; Defense proteins; Genetic variability.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000300016

Simulation of pattern of gene flow in Canjerana fragments in the Brazilian Atlantic Rainforest for evaluating genetic conservation strategies
Provedor de dados: 65
Autores: Serrote,Caetano Miguel Lemos; Reiniger,Lia Rejane Silveira; Costa,Leonardo Severo da; Stefanel,Charlene Moro; Silva,Karol Buuron da; Rabaiolli,Silvia Machado dos Santos.
ABSTRACT: Gene flow is important for the conservation of genetic resources to allow connectivity of geographically isolated populations and which genetic variability is reduced. Gene movement is a function of flow rate and model. Understanding how gene flow occurs can contribute to the conservation and selection of priority populations that could benefit from an eventual intervention. Simulation softwares allow making inferences about past events based on current datasets or predict future phenomena under real genetic scenarios. Adverse phenomena can be predicted and actions can be taken to avoid them. The aim of this study was to identify a model and the gene flow rates that could explain genetic structure of eight forest fragments of Cabralea canjerana...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cabralea canjerana; Reproductive isolation; Genetic variability; EASYPOP; Microsatellites.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800301

Development of rice seedlings grown from pre-hydrated seeds and irradiated with gamma rays
Provedor de dados: 64
Autores: Silva,Aline Scheer da; Danielowski,Rodrigo; Braga,Eugenia Jacira Bolacel; Deuner,Sidnei; Magalhães Junior,Ariano Martins de; Peters,Jose Antonio.
Techniques used to induce mutations, such as ionizing radiation, are an effective tool in increasing genetic variability in breeding programs of species of economic interest. This study aimed to evaluate the effect of different doses of Co60 gamma radiation on previously soaked seeds on the emergence and early growth of seedlings of two rice cultivars. To do this, seeds (25% moisture) of BRS Querência and BRS Fronteira were irradiated with zero (control), 50, 100, 150 and 200 Gy, and then sown in trays containing soil and kept in a greenhouse. The results showed that gamma radiation affected seedling development, where the highest dose tested (200 Gy) significantly reduced the emergence and the index of emergence speed. Growth parameters were reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa L; Humidity; Genetic variability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000600008

Drought tolerance evaluated in common bean genotypes
Provedor de dados: 64
Autores: Gonçalves,João Guilherme Ribeiro; Andrade,Estela Reis de; Silva,Daiana Alves da; Esteves,Jose Antonio de Fátima; Chiorato,Alisson Fernando; Carbonell,Sérgio Augusto Morais.
ABSTRACT Given the impact of climate issues and their direct influence on agricultural production, the aim of this study was to identify superior genotypes of dry edible common bean under water deficit. Thus, 30 common bean genotypes were evaluated under controlled greenhouse conditions in a randomized block experimental design with split plots and four replications; the plots consisted of the water treatments (irrigated and water deficit) and the split plots consisted of the genotypes. The results showed genetic variability among the accessions evaluated, and in spite of significant reduction in grain yield and stomatal conductance under water deficit, these two traits showed significant, positive correlation and are able to be applied in early selection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Genetic variability; Plant selection; Water deficit.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100211

Measurement of genetic diversity in progenies of sour passion fruit by ward-mlm methodology: a strategy for heterotic group formation
Provedor de dados: 64
Autores: Silva,Fernando Higino de Lima e; Viana,Alexandre Pio; Ferreira,Rulfe Tavares; Freitas,Jôsie Cloviane de Oliveira; Santos,Jardel Oliveira; Rodrigues,Daniele Lima.
Passion fruit belongs to the family Passifloraceae, and the genus Passiflora is regarded the most economically important. The present study aimed to quantify genetic diversity among progenies of sour passion fruit, in order to define potential heterotic groups, based on morpho-agronomic descriptors, using the Ward-MLM procedure. It is useful for generation advancement in the passion fruit breeding program, via recurrent selection, under development at the Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF). For such, 81 full-sib progenies belonging to the third recurrent selection cycle (C03) were assessed. Twenty-three descriptors were used, five of which were qualitative and 18, quantitative. The quantitative and qualitative variables were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora edulis Sims.; Genetic variability; Multivariate analysis; Characterization of germplasm.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000300003