Consulta: subjectFacets:"Genome size"
Registros recuperados: 10
Data/hora: 01/06/2024 17:39:03

Genome analysis of thirteen Colombian clostridial strains by pulsed field gel electrophoresis
Provedor de dados: Electronic Journal of Biotechnology
Autores: Quilaguy Ayure,Diana Milena; Suárez Moreno,Zulma Rocío; Aristizábal Gutierrez,Fabio Ancizar; Bernal Morales,Jose Mauricio; Montoya Castaño,Dolly.
Pulsed field gel electrophoresis was used for estimating the size of the genome and evaluating the presence of megaplasmids in 13 native Colombian solventogenic Clostridium strains. DNA preparation and purification were optimised for obtaining differentiated restriction fragments in electrophoresis. Genomic DNA was digested with ApaI, Eco52I, SmaI and XhoI enzymes. Estimated genome size for native strains ranged from 4.0 to 4.2 mega base pairs. Larger sized plasmids were detected and the presence of genes related to megaplasmid pSOL1 was determined by polymerase chain reaction. adc gene region amplification suggested that genes related to solventogenesis in native strains may be located in an extra-chromosomal element. Determining genome size provides...
Tipo: Journal article
Palavras-chave: Clostridium; Genome size; PFGE; PSOL1.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500009

Genome size of three Brazilian flies from the Sciaridae family
Provedor de dados: Genetics and Molecular Biology
Autores: Saccuti,Cecília Ferreira; Soares,Maria Albertina de Miranda; Falco,José Ricardo Penteado; Fernandez,Maria Aparecida.
We determined the genome size of the Brazilian sciarid flies Bradysia hygida, Rhynchosciara americana and Trichosia pubescens (Diptera: Sciaridae) using absorbance measurements of Feulgen-stained nuclei belonging to these species (and chicken erythrocytes as a standard) to calculate the amount of DNA in picograms (pg) and the number of base pairs (bp), or C-value, for each of these species. The C-values were: 3 x 10(8) bp (0.31 pg) for B. hygida; 3.6 x 10(8) bp (0.37 pg) for R. americana; and 1 x 10(9) bp (1.03 pg) for T. pubescens. The sciarids investigated in this work had considerably higher C-values than the average for previously described dipteran species, including D. melanogaster.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Genome size; Feulgen reaction; Sciaridae; Bradysia hygida; Rhynchosciara americana; Trichosia pubescens; Brazilian flies.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500015

Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Tavares,Mara Garcia; Carvalho,Carlos Roberto; Soares,Fernanda Aparecida Ferrari; Fernandes,Anderson.
When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetic; Flow cytometry; Genome size; Karyotype; Stingless bee.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300019

Karyotype and nuclear DNA content of hexa-, octo-, and duodecaploid lines of Bromus subgen. Ceratochloa
Provedor de dados: Genetics and Molecular Biology
Autores: Klos,Joanna; Sliwinska,Elwira; Kula,Adam; Golczyk,Hieronim; Grabowska-Joachimiak,Aleksandra; Ilnicki,Tomasz; Szostek,Krzysztof; Stewart,Alan; Joachimiak,Andrzej J.
The subgenus Ceratochloa of the genus Bromus includes a number of closely related allopolyploid forms or species that present a difficult taxonomic problem. The present work combines data concerning chromosome length, heterochromatin distribution and nuclear genome size of different 6x, 8x and 12x accessions in this subgenus. Special attention is paid to the karyotype structure and genomic constitution of duodecaploid plants recently found in South America. Hexaploid lineages possess six almost indistinguishable genomes and a nuclear DNA content between 12.72 pg and 15.10 pg (mean 1Cx value = 2.32 pg), whereas octoploid lineages contain the same six genomes (AABBCC) plus two that are characterized by longer chromosomes and a greater DNA content (1Cx = 4.47...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-banding; Heterochromatin; Flow cytometry; Genome size.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300016

Recent advances in EST sequencing in Crassostrea gigas: towards the sequencing of the Pacific oyster genome
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boudry, Pierre; Huvet, Arnaud; Lapegue, Sylvie; Sauvage, Christopher; Tanguy, A.; Moraga, D.; Goostrey, A.; Prunet, P.; Boulo, Viviane; Gueguen, Y.; Moal, Jeanne; Fleury, Elodie; Samain, Jean-francois; Mathieu, M.; Favrel, P.
Rationale for EST and genome sequencing of the Pacific oyster: • its membership of the Lophotrochozoa, an little studied branch of the Eukaryotes, • its worldwide aquacultural importance (4.2 million metric tons, worth 3.5 billion US dollars), • its high fecundity, with concomitantly high DNA polymorphism, • its use as a biosensor of coastal pollution. In 2004, an international community of biologists, the Oyster Genome Consortium (OGC) led by D. Hedgecock (University of Southern California) and uniting 70 participants from 10 countries, first presented the Pacific oyster Crassostrea gigas as a genome-sequencing candidate to Joint Genome Institute (JGI, USA) (Hedgecock et al., 2005). Besides this project (that remains to be accepted), several EST...
Tipo: Text
Palavras-chave: Characteristics; Genome size; Genome; Crassostrea gigas; Oyster; Genetic.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3414.pdf

Het Dryopteris affinis-complex in Nederland
Provedor de dados: Naturalis Digital Academic Repository
Autores: Hovenkamp, P.; Hendrikx, B.; Roskam, H.; Winter, W. de.
With the help of flow-cytometric analysis of nuclear DNA content and morphology, we establish the presence in the Netherlands of two taxa of the Dryopteris affinis complex: diploid D. affinis subsp. affinis, triploid D. affinis subsp. borreri, and the hybrids D × complexa (tetraploid) and D. × critica (pentaploid).  Onderzoek aan kern-DNA gewicht en morfologie van recente vondsten van het Dryopteris affinis-complex wijst uit dat in Nederland twee taxa en twee hybrides met redelijke zekerheid zijn gevonden: dediploïde D. affinis subsp. affinis, de triploïde D. affinis subsp. borreri, en de hybriden D × complexa (tetraploïd) en D. × critica (pentaploïd). We analyseren een aantal onderscheidende kenmerken en maken de keuze om deze taxa te behandelen als...
Tipo: Article / Letter to the editor
Palavras-chave: Dryopteris affinis; Soortscomplex; Species complex; Uitbreiding; Range expansion; Genoomgrootte; Genome size; Cytotypes; Polyploidie; Polyploidy; Morfometrie; Morphometrics.
Ano: 2018
URL: http://www.repository.naturalis.nl/record/669050

DNA content estimation of Fig and Black Mulberry using flow cytometry
Provedor de dados: Revista Brasileira de Fruticultura
Autores: Dalkiliç,Zeynel; Dalkiliç,Gonca Günver.
Abstract  In this study, fig and black mulberry DNA contents were estimated using DAPI fluorescence stain in flow cytometry. The 2C DNA contents of the fig and black mulberry were found as 0.82 pg and 8.34 pg, respectively. The calculated 1C value of genome size of fig is 401.8 Mbp and that of black mulberry is 4086.6 Mbp. The ratio of 2C DNA content and 1C genome of the black mulberry was 10.17 times that of the fig although fig is diploid and black mulberry is decosaploid.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ficus carica; Morus nigra; DAPI; DNA content; Flow cytometry; Genome size.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000200801

Cytogenetics of Chilean angiosperms: Advances and prospects
Provedor de dados: Revista Chilena de Historia Natural
Autores: JARA-SEGUEL,PEDRO; URRUTIA,JONATHAN.
Cytogenetic data on Chilean angiosperms have been reported since at least eight decades ago; however, much of this information is disperse in diverse sources and is not readily available as a comprehensive document that allows having a general vision on advances and gaps in this matter. The goal of this paper is to summarize the advances and prospets on cytogenetic studies of the Chilean angiosperms based on compiled publications from 1929 to 2010. We found 78 publications supplied by four groups of Chilean researchers and some foreign specialists. Cytogenetic data have been reported for 139 Chilean angiosperm species (2.8 % of the total), which belong to 58 genera and 34 families. During 2001-2010 there was an increase in the number of publications, being...
Tipo: Journal article
Palavras-chave: Chromosome banding; Chromosome number; Genome size; Karyotype morphology.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2012000100001

Genome size and ploidy variations in pearl millet (Pennisetum glaucum) and napier grass (Pennisetum purpureum) genotypes
Provedor de dados: Acta Agronómica
Autores: Animasaun,David Adedayo; Morakinyo,Joseph Akintade; Mustapha,Oba Toyin; Krishnamurthy,Rama.
Abstract Pennisetum glaucum (Pearl millet) and P. purpureum (Napier grass) are economically important members of the genus  Pennisetum . The knowledge of variability in chromosome number, size and genomic content of the species could provide clues on the mechanisms responsible for decrease or increase in genomic content in the evolutionary pathway. In the present study, twenty nine genotypes consisting of 24 pearl millet and 5 Napier grass were assessed for inter- and intra-variations in chromosome number, ploidy status and genome content. Conventional cytogenetic was used for chromosome counting and flow cytometry technique for assessing genomic contents of the genotypes. Pearl millet genotypes were diploid (2n = 14) while Napier grass were mainly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genome size; Flow cytometry; Napier grass; Pearl millet; Pennisetum; Ploidy analysis.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122019000400299

rDNA mapping, heterochromatin characterization and AT/GC content of Agapanthus africanus (L.) Hoffmanns (Agapanthaceae)
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: REIS,ARYANE C.; FRANCO,ANA LUIZA; CAMPOS,VICTÓRIA R.; SOUZA,FLÁVIA R.; ZORZATTO,CRISTIANE; VICCINI,LYDERSON F.; SOUSA,SAULO M.
ABSTRACT Agapanthus (Agapanthaceae) has 10 species described. However, most taxonomists differ respect to this number because the great phenotypic plasticity of the species. The cytogenetic has been an important tool to aid the plant taxon identification, and to date, all taxa of Agapanthus L'Héritier studied cytologically, presented 2n = 30. Although the species possess large chromosomes, the group is karyologically little explored. This work aimed to increase the cytogenetic knowledge of Agapanthus africanus (L.) Hoffmanns by utilization of chromosome banding techniques with DAPI / CMA3 and Fluorescent in situ Hybridization (FISH). In addition, flow cytometry was used for determination of DNA content and the percentage of AT / GC nitrogenous bases....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: African lily; Chromosome banding; Cytotaxonomy; Flow cytometry; Genome size; Molecular cytogenetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501727