Consulta: subjectFacets:"Gus"
Registros recuperados: 2
Data/hora: 10/06/2024 08:15:22

Co-transformation of a tropical maize endophytic isolate of Fusarium verticillioides (synonym F. moniliforme) with gusA and nia genes
Provedor de dados: Genetics and Molecular Biology
Autores: Pamphile,João A.; Rocha,Carmen Lúcia M.S.C. da; Azevedo,João L.
A tropical endophytic isolate of the fungus Fusarium verticillioides (synonym Fusarium moniliforme) obtained from Zea mays was co-transformed with plasmid pNH24 containing the Fusarium oxysporum nitrate reductase nia gene and plasmid pNOM 102 carrying the Escherichia coli b-glucuronidase gusA gene. Transformation frequency for the nia marker was 75 transformants mg-1 vector DNA and introduction of the gusA gene by co-transformation was 57.2% as indicated by the presence of the GUS+ phenotype on plates. Southern analyses confirmed the integration of both plasmids into the genome of ten GUS+ transformants. All co-transformants showed mitotic stability in respect of the GUS+ phenotype. To assess the potential of transformed endophytic fungi as vectors for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-transformation; Gus; Nia; Fusarium verticillioides; Endophyte.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200021

Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: CABRAL,GLAUCIA B.; CARNEIRO,VERA T.C.; GOMES,ANA CRISTINA M.M.; LACERDA,ANA LUIZA; MARTINELLI,ADRIANA P.; DUSI,DIVA M.A.
ABSTRACT Brachiaria brizantha is a forage grass well adapted to tropical areas and cultivated in millions of hectares in Brazil. The apomictic mode of reproduction in this species, in addition to differences in ploidy between sexual and apomictic plants, impairs crossbreeding. The development of a methodology to transform apomictic cultivars will provide an option to introduce agronomic important traits to B. brizantha cv. Marandu. In addition, it will open the possibility to study in vivo the function of candidate genes involved in the apomictic reproduction. The objective of this work was to evaluate peeled seeds, isolated embryo from mature seeds, embryogenic calluses and embryogenic cell suspensions, as target explant for genetic transformation via...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Forage; Gus; HptII; Hygromycin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401789