Consulta: subjectFacets:"HPLC analysis"
Registros recuperados: 3
Data/hora: 06/06/2024 04:44:44

Antioxidant flavonols from fruits, vegetables and beverages: measurements and bioavailability
Provedor de dados: Biological Research
Autores: CROZIER,ALAN; BURNS,JENNIFER; AZIZ,AZLINA A; STEWART,AMANDA J; RABIASZ,HELENA S; JENKINS,GARETH I; EDWARDS,CHRISTINE A; LEAN,MICHAEL EJ.
Flavonols are polyphenolic secondary plant metabolites that are present in varying levels in commonly consumed fruits, vegetables and beverages. Flavonols have long held an interest for nutritionists, which has increased following a Dutch study in the early 1990’s showing that dietary intake of flavonols was inversely correlated with the incidence of coronary heart disease. The main factors that have hindered workers in the field of flavonol research are (i) the accurate measurement of these compounds in foods and biological samples, and (ii) a dearth of information on their absorption and metabolism. This review aims to highlight the work of the authors in attempting to clarify the situation. The sensitive and selective HPLC procedure to identify and...
Tipo: Journal article
Palavras-chave: Bioavailability; Dietary antioxidants; Flavones; Flavonols; HPLC analysis.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200007

Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysis
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Kapnoor,Shankar; Mulimani,Veerappa Hanumanth.
α-Galactosidase was produced by Aspergillus oryzae on red gram plant waste-wheat bran based media in solid-state fermentation (SSF). Optimum temperature for α-galactosidase production was 35 0C and upto 4 cm of bed height of substrate had no inhibitory effect on enzyme production. Hydrolysis of galactooligosaccharides in soymilk was carried out by α-galactosidase. Optimum temperature and pH for the hydrolysis of raffinose and stachyose of soymilk were 55(0)C and 5.2-6.2, respectively. The enzymatic treatment for 3 h completely removed the raffinose oligosaccharides in soymilk. Crude extract also showed considerable amount of invertase activity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus oryzae; Α-galactosidase; SSF; Soymilk; Invertase; HPLC analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100026

Sustainable production with obtaining glucosamine from crab exoskeletons
Provedor de dados: Ciência Rural
Autores: Montiel-Montoya,Jorge; Valdez-Morales,Maribel; Reyes,Cesar; Barrales-Cureño,Hebert Jair.
ABSTRACT:  Two chemical treatments, five enzymatic (pectinase, lipase, hemicellulase, hemicellulose-cellulase or lipase-pectinase) and one microbiological (Bacillus subtilis) treatment were evaluated to obtain glucosamine hydrochloride (Gluc-HCl) from the chitin obtained from crab (Callinectes bellicosus) exoskeletons. Chemical treatments were referred as Method A (HCl hydrolysis during 75 min at 90°C) and Method B (HCl hydrolysis during 20 min and 14 h of rest). Glucosamine and, in some cases, N-acetyl-D-glucosamine were identified and quantified by HPLC. Treatments with the greater concentrations of Gluc-HCl in descending order were: lipase (94.4 mg/g), microbiological (45.7 mg/g), lipase-pectinase (22.9 mg/g), hemicellulase-cellulase (20.9 mg/g),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callinectes bellicosus; Extraction methods; Glucosamine hydrochloride; HPLC analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900752