Consulta: subjectFacets:"Hamsters"
Registros recuperados: 7
Data/hora: 08/06/2024 04:24:21

Response activity of alveolar macrophages in pulmonary dysfunction caused by Leptospira infection
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Marinho,M.; Oliveira-Júnior,I. S.; Perri,S. H.V; Peiró,J. R.; Pavanelli,T. F.; Salomão,R.
Leptopspirosis is a syndrome with different clinical manifestations including the most severe and often fatal forms of pulmonary disease of unknown etiology. Pulmonary injury during the inflammatory process has been associated with the excessive number of alveolar macrophages (AMs) and polymorphonuclear leukocytes stimulated in the lungs and with the production of reactive oxygen and nitrogen intermediates and other inflammatory mediators. The aim of the present work was to evaluate the cellular immune response of AMs or inflammatory cells of hamsters during leptospirosis. The activity of AMs was determined by measuring nitric oxide (NO) and protein production as well as inflammatory cell infiltration in bronchoalveolar lavage (BAL) fluid. Pulmonary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alveolar macrophages; Bronchoalveolar lavage; Gasometry; Pulmonary dysfunction; Hamsters; Leptospira.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100005

Influência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus) experimentalmente infectados com Leptospira interrogans sorovar pomona
Provedor de dados: Brazilian Jounarl of Veterinary
Autores: Macedo,Nicodemos Alves de; Morais,Zenáide Maria de; Camargo,Cláudio Roberto de Almeida; Alves,Clebert José; Azevedo,Sérgio Santos de; Nümberger Júnior,Rodolfo; Vasconcellos,Sílvio Arruda.
Foi investigada a influência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus) experimentalmente infectados com Leptospira interrogans sorovar pomona. As vias de inoculação ensaiadas foram: intraperitoneal, subcutânea, oral, conjuntival e escarificação cutânea. O inóculo infeccioso foi constituído por uma cultura em meio de Fletcher, com 20 a 30 leptospiras por campo microscópio no aumento de 200 vezes. Os animais controle foram inoculados apenas com meio de Fletcher. Foram colhidas amostras de soro sanguíneo e fragmentos de rins na fase agônica da doença ou no 21º dia pós-infecção, quando todos os animais foram sacrificados. Para a pesquisa de leptospiras, foi feito o exame direto com microscopia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Via de inoculação; Hamsters; Sorovar pomona.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000300008

Effect of two ram sperm capacitating media on acrosome reaction and zona-free hamster oocyte penetration test
Provedor de dados: Brazilian Jounarl of Veterinary
Autores: FERRARI,Silvia; BARNABE,Valquíria Hyppolito; ZÜGE,Roberta Mara; ZOGNO,Maria Amélia.
An in vitro zona-free hamster oocyte penetration test was utilized in 24 trials in order to evaluate the capacitation media used for ram sperm. A pool of fresh semen was collected from three crossed breed rams. Two semen drops were washed by centrifugation and incubated in high ionic strength treatment (HIS) or in a defined medium with HEPES, on heat ewe serum and heparin. After the incubation to promote capacitation, simplified triple-stain technique was used to evaluate the spontaneous acrosome reaction of the capacitated sperm. Superovulation in 96 golden hamsters was induced by PMSG and hCG. The oocytes were treated with hyaluronidase and trypsin to remove, respectively, the cumulus cells and the zona pellucida. Oocytes and capacitated sperm were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capacitation; Spermatozoa; Rams; Oocytes; Hamsters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000300010

Effect of different anesthetic agents on left ventricular systolic function assessed by echocardiography in hamsters
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Tanaka,D.M.; Romano,M.M.D.; Carvalho,E.E.V.; Oliveira,L.F.L.; Souza,H.C.D.; Maciel,B.C.; Salgado,H.C.; Fazan-Júnior,R.; Simões,M.V.
Determination of left ventricular ejection fraction (LVEF) using in vivo imaging is the cardiac functional parameter most frequently employed in preclinical research. However, there is considerable conflict regarding the effects of anesthetic agents on LVEF. This study aimed at assessing the effects of various anesthetic agents on LVEF in hamsters using transthoracic echocardiography. Twelve female hamsters were submitted to echocardiography imaging separated by 1-week intervals under the following conditions: 1) conscious animals, 2) animals anesthetized with isoflurane (inhaled ISO, 3 L/min), 3) animals anesthetized with thiopental (TP, 50 mg/kg, intraperitoneal), and 4) animals anesthetized with 100 mg/kg ketamine plus 10 mg/kg xylazine injected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echocardiography; Anesthetics; Systolic function; Hamsters.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000602

Comparison of Pavlovian serial conditional discrimination in rats and hamsters in the same experimental situation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bueno,J.L.O.; Figueiredo,T.H.; Mello,F.M.C.
The present study compares behavioral changes between two distinct rodent groups, hamsters (Mesocricetus auratus) and Wistar rats, when submitted in the same homogeneous experimental situations to a serial conditional discrimination procedure which involves water deprivation and the processing of temporal variables. Both hamsters and rats acquired serial positive conditional discrimination as indicated by higher frequencies of magazine-oriented behavior during the tone followed by reinforcement (T+) and preceded by the feature stimulus light (L) and during the empty interval, than during the tone alone not followed by reinforcement (T-). Rats' frequencies of magazine-oriented behavior were high during T+ and T-, initially during training, and decreased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conditional discrimination; Hamsters; Wistar rats; Behavior repertoire.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200012

Cross-protection between experimental anti-leptospirosis bacterins
Provedor de dados: Brazilian Journal of Microbiology
Autores: Dib,Cristina Corsi; Gonçales,Amane Paldês; Morais,Zenaide Maria de; Souza,Gisele Oliveira de; Miraglia,Fabiana; Abreu,Patricia Antonia Estima; Vasconcellos,Silvio Arruda.
We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pomona; Hamsters; Bacterin; Cross-protection.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300042

Cross neutralizing antibodies in hamsters vaccinated with leptospiral bacterins produced with three serovars of serogroup Sejroe
Provedor de dados: Brazilian Journal of Microbiology
Autores: Tabata,Rosana; Scanavini Neto,Hugo; Zuanaze,Marcelo Alexandre Fagnani; Oliveira,Eugenia Márcia de Deus; Dias,Ricardo Augusto; Morais,Zenaide Maria de; Ito,Fumio Honma; Vasconcellos,Silvio Arruda.
Three leptospiral bacterins, produced with different serovars of Serogroup Sejroe, namely the hardjo (bacterin A), wolffi (bacterin B) and guaricura (bacterin C), were evaluated in male hamsters (Mesocricetus auratus) by comparing the agglutinating and neutralizing antibodies titers using microscopic agglutination (MAT) and in vitro growth inhibition (GIT) tests. The immunization schedule was based on two 1.0 mL doses of non-diluted formalininactivated whole culture bacterin given through subcutaneous route with 10-day interval. The challenge was performed ten days after the second vaccine dose, when the animals were inoculated with 0.2 mL of non-inactivated cultures of each serovar through intraperitoneal route. On the 21st post-challenge day (PCD), all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Bacterins; Hamsters; Neutralizing antibodies; Serogroup Sejroe.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300016