Consulta: subjectFacets:"Heparin"
Registros recuperados: 12
Data/hora: 12/06/2024 07:26:50

Effects of a sulfated exopolysaccharide produced by Altermonas infernus on bone biology
Provedor de dados: 5
Autores: Velasco, C. Ruiz; Baud'Huin, M.; Sinquin, Corinne; Maillasson, M.; Heymann, D.; Colliec-jouault, Sylvia; Padrines, M.
The growth and differentiation of bone cells is controlled by various factors which can be modulated by heparan sulphates. Here, we investigated the effects of an oversulphated exopolysaccharide (OS-EPS) on bone. We compared the effect of this compound with that of a native exopolysaccharide (EPS). Long-term administration of OS-EPS causes cancellous bone loss in mice due, in part, to an increase in the number of osteoclasts lining the trabecular bone surface. No significant difference in cancellous bone volume was found between EPS-treated mice and age-matched control mice, underlying the importance of sulphation in trabecular bone loss. However, the mechanism sustaining this osteoporosis was unclear. To clarify OS-EPS activities, we investigated the...
Tipo: Text
Palavras-chave: Bone metabolism; Bone remodeling; Exopolysaccharide; Glycosaminoglycan; Heparin.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00032/14300/15749.pdf

Effect of Low Molecular Weight Fucoidan and Low Molecular Weight Heparin in a Rabbit Model of Arterial Thrombosis
Provedor de dados: 5
Autores: Durand, E; Helley, D; Zen, A; Dujols, C; Bruneval, P; Colliec-jouault, Sylvia; Fischer, Aurelie; Lafont, A.
Background: Therapeutic use of unfractionated heparin and low molecular weight heparins (LMWHs) is limited by hemorrhagic adverse effects. We compared the antithrombotic effect of LMW fucoidan (LMWF) and LMWH in an experimental model. Methods: Thrombosis was induced in femoral arteries of male New Zealand White rabbits by in situ induction of endothelial apoptosis with staurosporine (10(-5) M for 30 min). Starting the day before apoptosis induction, the animals received subcutaneous LMWF (15 mg/kg), LMWH (enoxaparin 2.5 mg/kg) or saline solution (control group) twice a day for 4 days. Results: The degrees of apoptosis and endothelial denudation were similar in the 3 groups. The thrombotic score was significantly lower in the LMWF group than in the LMWH and...
Tipo: Text
Palavras-chave: Tissue factor; Tissue factor pathway inhibitor; Heparin; Fucoidan; Thrombosis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4744.pdf

Heparin and commercial bothropic antivenom against the paralyzing effect of Bothrops jararacussu snake venom
Provedor de dados: 80
Autores: Rostelato-Ferreira,S; Rodrigues-Simioni,L; Oshima-Franco,Y.
The crude venom of Bothrops jararacussu (Bjssu) is known to induce muscular paralysis in vitro. Many studies have shown that various substances, including heparin, neutralize the damage caused by snake venom. In the present study, the ability of heparin (Hep) and commercial bothropic antivenom (CBA) to neutralize neuromuscular effects of Bjssu venom, at different time-points, was analyzed. Mouse phrenic nerve-diaphragm preparation was used through a conventional myographic technique, following five different protocols: Group 1 was incubated with Bjssu (40 µg/mL) without any other treatment; Groups 2 and 3 were pretreated with heparin (1 µL/mL) and CBA (120 µL/mL), respectively, for 15 minutes before venom addition; Group 4 after 50% neuromuscular blockade...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Heparin; Neutralization; Nerve-muscle preparation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100005

Structure and anticoagulant properties of sulfated glycosaminoglycans from primitive Chordates
Provedor de dados: 42
Autores: PAVÃO,MAURO S. G.
Dermatan sulfates and heparin, similar to the mammalian glycosaminoglycans, but with differences in the degree and position of sulfation were previously isolated from the body of the ascidian Styela plicata and Ascidia nigra. These differences produce profound effects on their anticoagulant properties. S. plicata dermatan sulfate composed by 2-O-sulfatedalpha-L-iduronic acid and 4-O-sulfated N-acetyl-beta-D-galactosamine residues is a potent anticoagulant due to a high heparin cofactor II activity. Surprisingly, it has a lower potency to prevent thrombus formation on an experimental model and a lower bleeding effect in rats than the mammalian dermatan sulfate. In contrast, A. nigra dermatan sulfate, also enriched in 2-O-sulfated alpha-L-iduronic acid, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascidian; Heparin; Dermatan sulfate; Heparin cofactor II; Anticoagulant; Antithrombotic.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000100007

The specificity of interactions between proteins and sulfated polysaccharides
Provedor de dados: 42
Autores: Mulloy,Barbara.
Sulfated polysaccharides are capable of binding with proteins at several levels of specificity. As highly acidic macromolecules, they can bind non-specifically to any basic patch on a protein surface at low ionic strength, and such interactions are not likely to be physiologically significant. On the other hand, several systems have been identified in which very specific substructures of sulfated polysaccharides confer high affinity for particular proteins; the best-known example of this is the pentasaccharide in heparin with high affinity for antithrombin, but other examples may be taken from the study of marine invertebrates: the importance of the fine structure of dermatan sulfate (DS) to its interaction with heparin cofactor II (HCII), and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sulfated polysaccharides; Fucans; Dermatan sulfate; Heparan sulfate; Heparin; Protein structure; Growth factors; Development.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000400007

Clinical biochemistry of rescued Magellanic Penguins (Spheniscus magellanicus): serum or heparinized plasma?
Provedor de dados: 45
Autores: Morais,J.; Volpato,J.; Costa,A.; Kolesnikovas,C.; Souza,M.; Saito,M.
ABSTRACT In the last few years, an increasing number of debilitated Magellanic penguins (Spheniscus magellanicus) has been rescued and taken to rehabilitation centers on Brazil’s southern coast to be clinically treated and evaluated for re-introduction. This work aims to compare the viability of heparinized plasma with the viability of serum for biochemistry analyses under rehabilitation conditions. Blood sampled from 31 physically healthy rescued penguins was processed into serum/plasma-paired samples and analyzed for 12 biochemical parameters: alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), cholesterol (CHOL), creatine kinase (CK), gamma-glutamyl transpeptidase (GGT), glucose, (GLU) lactate dehydrogenase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penguin; Rehabilitation; Blood sampling; Heparin; Optimization of resources.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300782

Proacrosin-acrosin activity in capacitated and acrosome reacted sperm from cryopreserved bovine semen
Provedor de dados: 47
Autores: Rosatti,M. I.; Beconi,M. T.; Córdoba,M.
Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-a-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acrosin at pH 8. Capacitation with heparin and quercitin failed to induce conversion of proacrosin to acrosin. An increase in acrosin activity produced by the presence of progesterone, in a dose-dependent manner, was related with the induction of true acrosome reaction. The total level of acrosin activity registered showed that 96% of acrosin of capacitated sperm samples and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Bovine sperm; Proacrosin-acrosin; Heparin; Quercitin; Progesterone.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300007

The functional links between prion protein and copper
Provedor de dados: 48
Autores: VARELA-NALLAR,LORENA; TOLEDO,ENRIQUE M; CHACÓN,MARCELO A; INESTROSA,NIBALDO C.
Prion diseases are fatal neurodegenerative disorders associated with the conversion of the cellular prion protein (PrPC) into a pathologic isoform. Although the physiological function of PrPC remains unknown, evidence relates PrPC to copper metabolism and oxidative stress as suggested by its copper-binding properties in the N-terminal octapeptide repeat region. This region also reduces copper ions in vitro, and this reduction ability is associated with the neuroprotection exerted by the octarepeat region against copper in vivo. In addition, the promoter region of the PrPC gene contains putative metal response elements suggesting it may be regulated by heavy metals. Here we address some of the evidence that support a physiological link between PrPC and...
Tipo: Journal article
Palavras-chave: Prion protein; Copper; Heparin; HSPG; Prnp; MRE.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100005

Effect of heparin on in vitro capacitation of boar sperm
Provedor de dados: 48
Autores: DAPINO,DORA G; MARINI,PATRICIA E; CABADA,MARCELO O.
Chlortetracycline (CTC) fluorescent pattern, the ability to undergo acrosome reaction (AR) upon exposure to 10 µM calcium ionophore A23187 and vitality estimation were used to investigate the effect of the sulfated glycosaminoglycan heparin on the in vitro capacitation of porcine spermatozoa. Sperm incubation in capacitating medium (CM) supplemented with 10 mM heparin for up to 120 min, showed an increase in the number of capacitated sperm (B pattern) and acrosome reacted sperm (AR pattern), without affecting their viability. In this condition, spermatozoa were incubated in CM depleted of albumin, calcium, bicarbonate or combinations, in the presence of heparin. In either calcium or bicarbonate-free media, capacitation was only basal and did not show...
Tipo: Journal article
Palavras-chave: Boar sperm; Capacitation; Heparin; Sulfated glycosaminoglycans.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500006

Development of new heparin-like compounds and other antithrombotic drugs and their interaction with vascular endothelial cells
Provedor de dados: 56
Autores: Nader,H.B.; Pinhal,M.A.S.; Baú,E.C.; Castro,R.A.B.; Medeiros,G.F.; Chavante,S.F.; Leite,E.L.; Trindade,E.S.; Shinjo,S.K.; Rocha,H.A.O.; Tersariol,I.L.S.; Mendes,A.; Dietrich,C.P.
The anticlotting and antithrombotic activities of heparin, heparan sulfate, low molecular weight heparins, heparin and heparin-like compounds from various sources used in clinical practice or under development are briefly reviewed. Heparin isolated from shrimp mimics the pharmacological activities of low molecular weight heparins. A heparan sulfate from Artemia franciscana and a dermatan sulfate from tuna fish show a potent heparin cofactor II activity. A heparan sulfate derived from bovine pancreas has a potent antithrombotic activity in an arterial and venous thrombosis model with a negligible activity upon the serine proteases of the coagulation cascade. It is suggested that the antithrombotic activity of heparin and other antithrombotic agents is due...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparin; Anticlotting activity heparan sulfate; Antithrombotic activity low molecular weight heparins; Preparation and antithrombotic activity antithrombotic agents; Development for clinical use crustacean heparin-like compounds; Antithrombotic activity antithrombotic agents vascular endothelial cells.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600002

P-selectin, carcinoma metastasis and heparin: novel mechanistic connections with therapeutic implications
Provedor de dados: 56
Autores: Varki,A.; Varki,N.M.
Metastasis is a multistep cascade initiated when malignant cells penetrate the tissue surrounding the primary tumor and enter the bloodstream. Classic studies indicated that blood platelets form complexes around tumor cells in the circulation and facilitate metastases. In other work, the anticoagulant drug heparin diminished metastasis in murine models, as well is in preliminary human studies. However, attempts to follow up the latter observation using vitamin K antagonists failed, indicating that the primary mechanism of heparin action was unrelated to its anticoagulant properties. Other studies showed that the overexpression of sialylated fucosylated glycans in human carcinomas is associated with a poor prognosis. We have now brought all these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparin; Carcinomas; P-selectin; Mucin; Metastasis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600003

In vivo antithrombotic properties of a heparin from the oocyte test cells of the sea squirt Styela plicata(Chordata-Tunicata)
Provedor de dados: 56
Autores: Cardilo-Reis,L.; Cavalcante,M.C.M.; Silveira,C.B.M.; Pavão,M.S.G.
In the ascidian Styela plicata, the oocytes are surrounded by two types of accessory cells named follicle cells and test cells. A heparin-like substance with an anticoagulant activity equivalent to 10% of mammalian heparin and about 5% as potent as the mammalian counterpart for the inhibition of thrombin by antithrombin was isolated from the oocyte test cells. In the present study, we compared the antithrombotic and hemorrhagic effects of sea squirt oocyte test cell heparin with those of porcine heparin in rat models of venous thrombosis and blood loss. Intravenous administration of the oocyte test cell heparin to Wistar rats (both sexes, weighing ~300 g, N = 4 in each group) at a dose of 5.0 mg/kg body weight, which produced a 1.8-fold increase in plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascidian; Heparin; Experimental thrombosis; Antithrombotics; Hemorrhage.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100004