Consulta: subjectFacets:"Hepatitis E virus"
Registros recuperados: 10
Data/hora: 09/06/2024 01:06:31

Detection of Hepatitis E Virus in Sewage After an Outbreak on a French Island
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Miura, Takayuki; Lhomme, Sebastien; Le Saux, Jean-claude; Le Mehaute, Philippe; Guillois, Yvonnick; Couturier, Elizabeth; Izopet, Jacques; Abranavel, Florence; Le Guyader, Soizick.
A hepatitis E outbreak, which occurred on a small isolated island, provided an opportunity to evaluate the association between the number of hepatitis E cases in the community and the concentration of virus detected in sewage. Samples were collected from the different sewage treatment plants from the island and analyzed for the presence of hepatitis E (HEV) virus using real-time RT-PCR. We demonstrated that if 1–4 % of inhabitants connected to a WWTP were infected with HEV, raw sewage contained HEV at detectable levels. The finding that such a small number of infected people can contaminate municipal sewage works raises the potential of the further distribution of the virus. Indeed, investigating the routes of transmission of HEV, including the potential...
Tipo: Text
Palavras-chave: Hepatitis E virus; Wastewater treatment plant; Quantification.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00334/44482/44199.pdf

High load of hepatitis E viral RNA in pork livers but absence in pork muscle at French slaughterhouses
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Feurer, C.; Le Roux, A.; Rossel, R.; Barnaud, E.; Dumarest, M.; Garry, Pascal; Pavio, N.
Pork ham muscle can be contaminated with HEV via blood vessels during viremia and represents a possible source of human contamination via the consumption of dried ham. This study evaluated the prevalence of HEV RNA in pork ham muscles and pork livers at slaughterhouses. Serology was determined on the corresponding serum samples. The apparent individual seroprevalence rate in the 49 pig farms studied was 59% [55.5%–61.4%]. None of the 1134 ham muscles tested was positive for the presence of HEV. HEV prevalence in paired liver samples was 2.8% with a level of contamination of up to 1.46 108 copies/g. Sequences of viral strains isolated from positive livers belonged to genotype 3 and subtypes 3c, 3e, 3f and 3j. Our results confirmed that raw pork liver food...
Tipo: Text
Palavras-chave: Pork muscles; Hepatitis E virus; Food safety.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00405/51647/52196.pdf

Molecular detection of hepatitis E virus in feces and slurry from swine farms, Rio Grande do Sul, Southern Brazil
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Vasconcelos,J.; Soliman,M.C.; Staggemeier,R.; Heinzelmann,L.; Weidlich,L.; Cimirro,R.; Esteves,P.A.; Silva,A.D.; Spilki,F.R.
Hepatitis E virus (HEV) is highly disseminated among swine herds worldwide. HEV is also a threat to public health, since particularly genotypes 3 and 4 may cause acute hepatitis in human beings. No previous studies were done on the occurrence of HEV in environmental samples in Rio Grande do Sul, Brazil. In the present study, reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect the presence of HEV in swine feces and in effluents from slurry lagoons in farms located in the municipality of Teutônia, inside the area of swine husbandry in the state. Pooled fecal samples from the floor of pig barns from 9 wean-to-finish farms and liquid manure samples were collected from the slurry lagoons from 8 of these farms. From the pooled fecal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis E virus; Swine HEV; Slurry; Zoonosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300777

Hepatitis E virus seroprevalence among schistosomiasis patients in Northeastern Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Passos-Castilho,Ana Maria; de Sena,Anne; Domingues,Ana Lucia Coutinho; Lopes-Neto,Edmundo Pessoa; Medeiros,Tibério Batista; Granato,Celso Francisco Hernandez; Ferraz,Maria Lúcia.
Abstract  Background Hepatitis E virus (HEV) can cause chronic infection with rapid progression to liver cirrhosis in immunocompromised patients. HEV seroprevalence in patients with Schistosoma mansoni in Brazil is unknown. We evaluated the prevalence of past or present HEV infection in schistosomiasis patients in Recife, Pernambuco, Brazil. A total of 80 patients with Schistosoma mansoni were consecutively enrolled in a cross-sectional study. Serum samples were tested for the presence of anti-HEV IgG antibodies by enzyme immunoassay (Wantai anti-HEV IgG, Beijing, China) and for the presence of HEV RNA using real time reverse transcriptase-polymerase chain reaction with primers targeting the HEV ORF2 and ORF3. Clinical and laboratory tests as well as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Epidemiology; Hepatitis E virus; Schistosomiasis; Seroprevalence.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300007

Seroprevalence of hepatitis E virus in chronic hepatitis C in Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Bricks,Guilherme; Senise,Jorge Figueiredo; Pott Junior,Henrique; Grandi,Giuliano; Passarini,Amanda; Caldeira,Débora Bellini; Carnaúba Junior,Dimas; Moraes,Hamilton Antonio Bonilha de; Granato,Celso Franscisco Hernandes; Castelo,Adauto.
ABSTRACT  Background and aims: Hepatitis E virus infection in patients with underlying chronic liver disease is associated with liver decompensation and increased lethality. The seroprevalence of hepatitis E virus in patients with chronic hepatitis C in Brazil is unknown. This study aims to estimate the seroprevalence of hepatitis E virus in patients with chronic hepatitis C and to describe associated risk factors.  Methods: A total of 618 patients chronically infected with hepatitis C virus from three reference centers of São Paulo, Brazil were included. Presence of anti-HEV IgG was assessed by enzyme-linked immunosorbent assay (WANTAI HEV-IgG ELISA).  Results: Out of the 618 patients tested, 10.2% turned out positive for anti-HEV IgG (95% CI 8.0–12.8%)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis E virus; Hepatitis C virus; Brazil; Seroprevalence.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200085

Previous hepatitis E virus infection, cirrhosis and insulin resistance in patients with chronic hepatitis C
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Bricks,Guilherme; Senise,Jorge Figueiredo; Pott-Jr,Henrique; Grandi,Giuliano; Carnaúba-Jr,Dimas; Moraes,Hamilton Antonio Bonilha de; Granato,Celso Franscisco Hernandes; Castelo,Adauto.
ABSTRACT  Background: Hepatitis E virus (HEV) infection in patients with pre-existing liver disease has shown high morbidity and lethality. The consequences of HEV superinfection in patients with chronic hepatitis C virus (HCV) infection are not fully understood. This study aimed to evaluate the association between the presence of anti-HEV antibodies, liver cirrhosis, and insulin resistance.  Methods: A total of 618 patients chronically infected with HCV were included from three reference centers for viral hepatitis in São Paulo, Brazil. Presence of anti-HEV IgG was assessed by enzyme-linked immunosorbent assay (WANTAI HEV-IgG ELISA).  Results: The seroprevalence of anti-HEV in patients with cirrhosis was significantly higher than in patients without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis E virus; Hepatitis C virus; Liver cirrhosis; Insulin resistance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100045

High prevalence of hepatitis E virus antibodies in Sao Paulo, Southeastern Brazil: analysis of a group of blood donors representative of the general population
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Passos-Castilho,Ana Maria; Reinaldo,Mônica Renata; Sena,Anne de; Granato,Celso F.H.
Abstract Brazil is a non-endemic country for hepatitis E virus (HEV) infection with seroprevalence from 1% to 4% in blood donors and the general population. However, data on seroprevalence of HEV in the country are still limited. This study evaluated the prevalence of past or present HEV infection in a group of blood donors representative of the general population of the city of Sao Paulo, Southeastern Brazil. Serum samples from 500 blood donors were tested from July to September 2014 by serological and molecular methods. Anti-HEV IgG antibodies were detected in 49 (9.8%) subjects and categorized age groups revealed an age-dependent increase of HEV seroprevalence. Among the anti-HEV IgG positive subjects, only 1 had anti-HEV IgM while none tested positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis E virus; Blood donors; Seroprevalence; Brazil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500535

Development and validation of a genotype 3 recombinant protein-based immunoassay for hepatitis E virus serology in swine
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: van der Poel,W.H.M.; Pavio,N.; van der Goot,J.; van Es,M.; Martin,M.; Engel,B.
Hepatitis E virus (HEV) is classified within the family Hepeviridae, genus Hepevirus. HEV genotype 3 (Gt3) infections are endemic in pigs in Western Europe and in North and South America and cause zoonotic infections in humans. Several serological assays to detect HEV antibodies in pigs have been developed, at first mainly based on HEV genotype 1 (Gt1) antigens. To develop a sensitive HEV Gt3 ELISA, a recombinant baculovirus expression product of HEV Gt3 open reading frame-2 was produced and coated onto polystyrene ELISA plates. After incubation of porcine sera, bound HEV antibodies were detected with anti-porcine anti-IgG and anti-IgM conjugates. For primary estimation of sensitivity and specificity of the assay, sets of sera were used from pigs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis E virus; Zoonosis; Swine; ELISA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400334

Molecular characterization of swine hepatitis E virus from Southeastern Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Paiva,Helder Henrique; Tzaneva,Valentina; Haddad,Rodrigo; Yokosawa,Jonny.
Despite serological evidences of presence of hepatitis E virus (HEV) in humans or other animals, until this study the virus had not been detected and molecular characterization of the isolate that circulates in Brazil had not been described. Thus, we collected stool samples of young pigs and tested for presence of HEV RNA by RT-PCR, using primers for partial amplification of ORF2 sequence. Phylogenetic analysis with sequence obtained from the amplified products revealed that the HEV isolate identified here was most closely related to HEV isolates of genotype 3, which is commonly detected in HEV infected pigs. Nucleotide sequence analyses carried out with the entire amplified fragment, ORF2/ORF3 overlapping and ORF2 non-overlapping sequences showed highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Viral hepatitis; Hepatitis E virus; HEV; Molecular virology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400020

High frequency of hepatitis E virus infection in swine from South Brazil and close similarity to human HEV isolates
Provedor de dados: Brazilian Journal of Microbiology
Autores: Passos-Castilho,Ana Maria; Granato,Celso Francisco Hernandes.
Abstract Hepatitis E virus is responsible for acute and chronic liver infections worldwide. Swine hepatitis E virus has been isolated in Brazil, and a probable zoonotic transmission has been described, although data are still scarce. The aim of this study was to investigate the frequency of hepatitis E virus infection in pigs from a small-scale farm in the rural area of Paraná State, South Brazil. Fecal samples were collected from 170 pigs and screened for hepatitis E virus RNA using a duplex real-time RT-PCR targeting a highly conserved 70 nt long sequence within overlapping parts of ORF2 and ORF3 as well as a 113 nt sequence of ORF2. Positive samples with high viral loads were subjected to direct sequencing and phylogenetic analysis. hepatitis E virus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Genotype 3; Hepatitis E virus; Pigs; Zoonosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200373