Consulta: subjectFacets:"Hepatocellular carcinoma"
Registros recuperados: 24
Data/hora: 09/06/2024 01:37:08

Association of polymorphisms in the heparanase gene (HPSE) with hepatocellular carcinoma in Chinese populations
Provedor de dados: 74
Autores: Yu,Lixia; Zhang,Xiaoai; Zhai,Yun; Zhang,Hongxing; Yue,Wei; Zhang,Xiumei; Wang,Zhifu; Zhou,Hong; Zhou,Gangqiao; Gong,Feng.
Abstract Heparanase activity is involved in cancer growth and development in humans and single nucleotide polymorphisms (SNPs) in the heparanase gene (HPSE) have been shown to be associated with tumors. In this study, we investigated whether SNPs in HPSE were a risk factor for hepatocellular carcinoma (HCC) by undertaking a comprehensive haplotype-tagging, case-control study. For this, six haplotype-tagging SNPs (htSNPs) in HPSE were genotyped in 400 HCC patients and 480 controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. A log-additive model revealed significant correlations between the HPSE polymorphisms rs12331678 and rs12503843 and the risk of HCC in the overall samples (p = 0.0046 and p = 0.0055). When...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic association; Heparanase; Hepatocellular carcinoma; HPSE gene; PCR-RFLP.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500743

Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells
Provedor de dados: 48
Autores: Doan,Chung Chinh; Le,Long Thanh; Hoang,Son Nghia; Do,Si Minh; Van Le,Dong.
BACKGROUND: Vascular endothelial growth factor (VEGF) is involved in the growth of new blood vessels that feed tumors and kinesin spindle protein (KSP) plays a critical role in mitosis involving in cell proliferation. Simultaneous silencing of VEGF and KSP, an attractive and viable approach in cancer, leads on restricting cancer progression. The purpose of this study is to examine the therapeutic potential of dual gene targeted siRNA cocktail on human hepatocellular carcinoma Hep3B cells. RESULTS: The predesigned siRNAs could inhibit VEGF and KSP at mRNA level. siRNA cocktail showed a further downregulation on KSP mRNA and protein levels compared to KSP-siRNA or VEGF-siRNA, but not on VEGF expression. It also exhibited greater suppression on cell...
Tipo: Journal article
Palavras-chave: Vascular endothelial cell growth factor (VEGF); Kinesin spindle protein (KSP); SiRNA cocktail; Proliferation; Apoptosis; Hepatocellular carcinoma.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100064

Effect of sustained virologic response on the incidence of hepatocellular carcinoma in patients with HCV cirrhosis
Provedor de dados: 55
Autores: Cheinquer,Nelson; Cheinquer,Hugo; Wolff,Fernando H; Coelho-Borges,Silvia.
BACKGROUND AND OBJECTIVES: Evidence suggests that sustained virologic response to interferon treatment decreases incidence of hepatocellular carcinoma in patients with hepatitis C virus cirrhosis. This study was designed to compare the incidence of hepatocellular carcinoma among cirrhotic patients exposed to interferon based treatment with or without achieving a sustained virological response, in order to evaluate the role of interferon itself in the prevention hepatocellular carcinoma. METHODS: A cohort of 85 patients with compensated hepatitis C cirrhosis was followed after treatment with interferon and ribavirin. Sustained virological response was defined as negative polymerase chain reaction assay 24 weeks after the end of treatment. Patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; HCV interferon; Sustained virologic response; Cirrhosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500006

Retrospective analysis of hepatitis B virus chronic infection in 247 patients: clinical stages, response to treatment and poor prognostic factors
Provedor de dados: 55
Autores: Cunha-Silva,Marlone; Marinho,Fábio R.T.; Oliveira,Paulo F.; Lopes,Tirzah M.; Sevá-Pereira,Tiago; Lorena,Sonia L.S.; Almeida,Jazon R.S.
Abstract  Background: Chronic hepatitis B is a major cause of cirrhosis, and the natural history of the disease has several clinical stages that should be thoroughly understood for the implementation of proper treatment. Nonetheless, curing the disease with antiviral treatment remains a challenge.  Aims: To describe the clinical course, response to treatment, and poor prognostic factors in 247 hepatitis B virus chronic infection patients treated in a tertiary hospital in Brazil.  Methods: This was a retrospective and observational study, by analyzing the medical records of HBV infected patients between January 2000 and January 2015.  Results: Most patients were male (67.2%) and 74.1% were HBeAg negative. Approximately 41% had cirrhosis and 8.5% were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B; Clinical stages; Treatment; Liver cirrhosis; Hepatocellular carcinoma.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400441

Long-term follow-up of patients with chronic hepatitis C with sustained virologic response to interferon
Provedor de dados: 55
Autores: Ferreira,Sandro da Costa; Carneiro,Marcos de Vasconcelos; Souza,Fernanda Fernandes; Teixeira,Andreza Corrêa; Villanova,Marcia Guimarães; Figueiredo,José Fernando de Castro; Passos,Afonso Dinis Costa; Ramalho,Leandra Naira Zambelli; Zucoloto,Sergio; Martinelli,Ana de Lourdes Candolo.
BACKGROUND AND AIM: The durability of the sustained virologic response (SVR) in patients with chronic hepatitis C after treatment and the ideal follow-up time for these patients remains undefined. The objective of the study was to evaluate the durability of the virologic response in patients with chronic hepatitis C followed up for at least 12 months after SVR at HCFMRP-USP. METHODS: The study was conducted on 174 patients with chronic hepatitis C treated with different antiviral regimens who had achieved SVR. Qualitative serum HCV-RNA was determined by the commercial kit (COBAS AMPLICOR HCV, v2.0). RESULTS: There was predominance of male (73%) with a mean age of 45.6 ± 10 years. Liver cirrhosis was present in 16.1% of the study subjects. Mean follow-up...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Interferon; HCV; Follow-up; Hepatocellular carcinoma.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400003

Schistosomal liver fibrosis and hepatocellular carcinoma - case series of patients submitted to liver transplantation
Provedor de dados: 55
Autores: Filgueira,Norma Arteiro; Saraiva,Camilla Maria de Alencar; Jucá,Norma Thomé; Bezerra,Matheus Filgueira; Lacerda,Claudio Moura.
ABSTRACT Schistosomiasis affects approximately 207 million people in 76 countries. The association between hepatocellular carcinoma and Schistosoma mansoni infection has been investigated. Studies using animal models suggest that the parasite may accelerate the oncogenic process when combined with other factors, such as hepatitis C virus infection or exposure to a carcinogen. Herein, we report a case series of six hepatocellular carcinoma patients from Northeast Brazil, with negative serology for both hepatitis B and C virus, submitted to liver transplantation, whose explant showed evidence of schistosomal liver fibrosis. Since all patients enrolled in this study were submitted to liver transplantation, we were able to access the whole explanted liver and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Schistosoma mansoni; Hepatocellular carcinoma; Liver transplantation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400352

Coexistence of low levels of HBsAg and high levels of anti-HBs may increase risk of hepatocellular carcinoma in chronic hepatitis B patients with high HBV load
Provedor de dados: 55
Autores: Jin,Zi-zheng; Jin,Fang-fang; Liu,Xin; Liu,Ning; Wen,Feng; Lou,Jin-li.
ABSTRACT  Objective: The clinical significance of coexistence of HBsAg/anti-HBs in chronic hepatitis B (CHB) patients remains controversial. This study was aimed to assess the association of this serological pattern with hepatocellular carcinoma (HCC) in patients with CHB.  Methods: In this cross-section study, 206 CHB patients with coexistence of HBsAg/anti-HBs and 206 CHB patients with HBsAg alone were included to evaluate the risk of HCC development by logistic regression analysis. In addition, a retrospective cohort of 260 patients with CHB was recruited to estimate the cumulative incidence of HCC by Kaplan-Meier analysis.  Results: The serological pattern of coexistence of HBsAg/anti-HBs, with high levels of ("High") HBsAg/low levels of ("Low")...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B virus; HBsAg; Hepatocellular carcinoma.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500343

Antiproliferative effect of urolithin A, the ellagic acid-derived colonic metabolite, on hepatocellular carcinoma HepG2.2.15 cells by targeting Lin28a/let-7a axis
Provedor de dados: 56
Autores: Qiu,Zhenpeng; Zhou,Junxuan; Zhang,Cong; Cheng,Ye; Hu,Junjie; Zheng,Guohua.
An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. The RNA and protein expressions of targets were assessed by quantitative PCR and western blot, respectively. Results revealed that urolithin A induced cytotoxicity in HepG2.2.15 cells, which was accompanied by the cleavage of caspase-3 protein and down-regulation of Bcl-2/Bax ratio. Moreover, urolithin A suppressed the protein expressions of Sp-1, Lin28a,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urolithin A; Hepatocellular carcinoma; Lin28a; Let-7a; Cell proliferation; HBx.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700602

IL-6 upregulation contributes to the reduction of miR-26a expression in hepatocellular carcinoma cells
Provedor de dados: 56
Autores: Zhang,Yafei; Zhang,Bicheng; Zhang,Anran; Li,Xiaohua; Liu,Jian; Zhao,Jie; Zhao,Yong; Gao,Jianfei; Fang,Dianchun; Rao,Zhiguo.
A recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a expression was found to be transcriptionally downregulated by oncogene c-Myc in various cancers, and the expression of c-Myc was increased by IL-6 stimulation, we hypothesized that IL-6 contributes to reduction of miR-26a in hepatocellular carcinoma. Serum IL-6 was measured by ELISA and miR-26a was detected by qRT-PCR. The data of 30 patients with hepatocellular carcinoma who had undergone surgical tumor resection revealed that serum IL-6 could be considered to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-26a; IL-6; C-Myc; Hepatocellular carcinoma; Carcinoma prognosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100032

Low occurrence of occult hepatitis B virus infection and high frequency of hepatitis C virus genotype 3 in hepatocellular carcinoma in Brazil
Provedor de dados: 56
Autores: Alencar,R.S.M.; Gomes,M.M.S.; Sitnik,R.; Pinho,J.R.R.; Malta,F.M.; Mello,I.M.V.G.C.; Mello,E.S.; Bacchella,T.; Machado,M.C.C.; Alves,V.A.F.; Carrilho,F.J.
Occult hepatitis B virus (HBV) infection has been reported among patients with hepatitis C virus (HCV) infection and hepatocellular carcinoma (HCC). Our aim was to evaluate the presence of occult HBV infection in patients with HCV-related liver cirrhosis (LC) with or without HCC in São Paulo, Brazil. Serum and liver tissue samples from 50 hepatitis B surface antigen-negative patients with HCV-related LC who underwent liver transplantation at the University of São Paulo School of Medicine Hospital from 1993 to 2004 were divided into groups with LC only (N = 33) and with LC plus HCC (N = 17). HBV DNA was assayed for serum and paraffin-embedded liver tissue (tumoral and non-tumoral) using real time PCR and only 1 case with HCC had HBV DNA-positive serum. All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; Occult hepatitis B; Hepatocellular carcinoma; HCV genotypes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300010

Resistance of multicellular aggregates to pharmorubicin observed in human hepatocarcinoma cells
Provedor de dados: 56
Autores: Jianmin,Z.; Hongfang,W.; Meifu,F.
The objective of the present study was to investigate the multicellular resistance of human hepatocarcinoma cells BEL-7402 to pharmorubicin. Cells (1 x 10(4)) and 200 microcarrier Cytodex-3 beads were seeded onto a 24-well plate and cultured in RPMI 1640 medium. After the formation of multicellular aggregates, morphology and cell viability were analyzed by scanning electron microscopy, transmission electron microscopy and flow cytometry, respectively. The IC50 was determined by flow cytometry and MTT assay after the cells cultured in aggregates and monolayers were treated with pharmorubicin. The culture products exhibited structural characteristics somewhat similar to those of trabecular hepatocarcinoma in vivo. Among the microcarriers, cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Spheroids; Drug resistance; Microcarrier; Epirubicin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200015

Retrospective analysis of efficacy, safety, and prognostic factors in a cohort of Chinese hepatocellular carcinoma patients treated with drug-eluting bead transarterial chemoembolization
Provedor de dados: 56
Autores: Chen,Sihan; Ji,Rengbin; Shi,Xiaojun; Wang,Zhe; Zhu,Dedong.
The aim of our study was to assess the efficacy, safety, and prognostic factors of drug-eluting bead transarterial chemoembolization (DEB-TACE) in Chinese hepatocellular carcinoma (HCC) patients. Patients (n=102) diagnosed as primary HCC were consecutively enrolled in this retrospective cohort study. Treatment responses were assessed following the modified Response Evaluation Criteria in Solid Tumors. Progression-free survival (PFS) and overall survival (OS) were evaluated, and adverse events (AEs) as well as liver function-related laboratory indexes of all DEB-TACE records (N=131) were assessed. Complete response (CR) rate, objective response rate, and disease control rate were 51.0, 87.3, and 95.1%, respectively, at 1–3 months post DEB-TACE. The mean PFS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Drug-eluting bead transarterial chemoembolization; China; Treatment response; Survival; Adverse events.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200608

Does hepatocellular carcinoma in non-alcoholic steatohepatitis exist in cirrhotic and non-cirrhotic patients?
Provedor de dados: 56
Autores: Chagas,A.L.; Kikuchi,L.O.O.; Oliveira,C.P.M.S.; Vezozzo,D.C.P.; Mello,E.S.; Oliveira,A.C.; Cella,L.C.; Herman,P.; Bachella,T.; Caldwell,S.H.; Alves,V.A.F.; Carrilho,F.J.
Non-alcoholic steatohepatitis (NASH) has been associated with hepatocellular carcinoma (HCC) often arising in histologically advanced disease when steatohepatitis is not active (cryptogenic cirrhosis). Our objective was to characterize patients with HCC and active, histologically defined steatohepatitis. Among 394 patients with HCC detected by ultrasound imaging over 8 years and staged by the Barcelona Clinic Liver Cancer (BCLC) criteria, we identified 7 cases (1.7%) with HCC occurring in the setting of active biopsy-proven NASH. All were negative for other liver diseases such as hepatitis C, hepatitis B, autoimmune hepatitis, Wilson disease, and hemochromatosis. The patients (4 males and 3 females, age 63 ± 13 years) were either overweight (4) or obese...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Hepatocellular carcinoma; Liver cancer; Fatty liver; Steatohepatitis; Cirrhosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000014

Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway
Provedor de dados: 56
Autores: Wang,J.; Lu,M.L.; Dai,H.L.; Zhang,S.P.; Wang,H.X.; Wei,N.
This study investigated the in vitro and in vivo antiproliferative activity of esculetin against hepatocellular carcinoma, and clarified its potential molecular mechanisms. Cell viability was determined by the MTT (tetrazolium) colorimetric assay. In vivo antitumor activity of esculetin was evaluated in a hepatocellular carcinoma mouse model. Seventy-five C57BL/6J mice were implanted with Hepa1-6 cells and randomized into five groups (n=15 each) given daily intraperitoneal injections of vehicle (physiological saline), esculetin (200, 400, or 700 mg·kg-1·day-1), or 5-Fu (200 mg·kg-1·day-1) for 15 days. Esculetin significantly decreased tumor growth in mice bearing Hepa1-6 cells. Tumor weight was decreased by 20.33, 40.37, and 55.42% with increasing doses of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esculetin; Apoptosis; Hepatocellular carcinoma; Mitochondrial-dependent pathway; Antitumor activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300245

TIMP-1 mediates the inhibitory effect of interleukin-6 on the proliferation of a hepatocarcinoma cell line in a STAT3-dependent manner
Provedor de dados: 56
Autores: Guo,S.-Y.; Shen,X.; Yang,J.; Yuan,J.; Yang,R.-L.; Mao,K.; Zhao,D.-H.; Li,C.-J.
The tissue inhibitor of metalloproteinases (TIMP)-1 is a multifunctional protein which is not only an inhibitor of matrix metalloproteinases (MMPs) but also to have a possible "cytokine-like" action. Here, we first compared mRNA expression of TIMP-1 and MMP-9 in BEL-7402 (a hepatocellular carcinoma cell line), L-02 (a normal liver cell line) and QSG-7701 (a cell line derived from peripheral tissue of liver carcinoma) using real-time quantitative RT-PCR. By evaluating the variation of the MMP-9/TIMP-1 ratio as an index of reciprocal changes of the expression of the two genes, we observed that the MMP-9/TIMP-1 ratio was about 13- and 5-fold higher in BEL-7402 than in L-02 and QSG-7701, respectively. Significantly, overexpression of TIMP-1 decreased the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue inhibitor of metalloproteinases-1; Hepatocellular carcinoma; Interleukin-6; STAT3; P21.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500004

Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition
Provedor de dados: 56
Autores: Zhou,Yuan; Xue,Ruifeng; Wang,Jinglin; Ren,Haozhen.
Hepatocellular carcinoma (HCC) is one of the most common primary malignant tumors of the liver worldwide. Liver resection and transplantation are currently the only effective treatments; however, recurrence and metastasis rates are still high. Previous studies have shown that the epithelial-mesenchymal transition (EMT) is a key step in HCC invasion and metastasis. Inhibition of EMT has become a new therapeutic strategy for tumors. Recently, puerarin, a well-characterized component of traditional Chinese medicine, has been isolated from Pueraria radix and exerts positive effects on many diseases, particularly cancers. In this study, CCK-8, EdU immunofluorescence, colony formation, wound healing, and migration assays were used to detect the effects of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Metastasis; Epithelial-mesenchymal transition; PTEN; MiR-21.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400611

Differential gene expression profiles of hepatocellular carcinomas associated or not with viral infection
Provedor de dados: 56
Autores: Bellodi-Privato,M.; Kubrusly,M.S.; Stefano,J.T.; Soares,I.C.; Wakamatsu,A.; Oliveira,A.C.; Alves,V.A.F.; Bacchella,T.; Machado,M.C.C.; D’Albuquerque,L.A.C.
Chronic hepatitis B (HBV) and C (HCV) virus infections are the most important factors associated with hepatocellular carcinoma (HCC), but tumor prognosis remains poor due to the lack of diagnostic biomarkers. In order to identify novel diagnostic markers and therapeutic targets, the gene expression profile associated with viral and non-viral HCC was assessed in 9 tumor samples by oligo-microarrays. The differentially expressed genes were examined using a z-score and KEGG pathway for the search of ontological biological processes. We selected a non-redundant set of 15 genes with the lowest P value for clustering samples into three groups using the non-supervised algorithm k-means. Fisher’s linear discriminant analysis was then applied in an exhaustive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Molecular biomarkers; Viral infection; Oligo-microarrays.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200001

Diagnosis, staging and treatment of hepatocellular carcinoma
Provedor de dados: 56
Autores: França,A.V.C.; Elias Junior,J.; Lima,B.L.G.; Martinelli,A.L.C.; Carrilho,F.J.
Hepatocellular carcinomas are aggressive tumors with a high dissemination power. An early diagnosis of these tumors is of great importance in order to offer the possibility of curative treatment. For an early diagnosis, abdominal ultrasound and serum alpha-fetoprotein determinations at 6-month intervals are suggested for all patients with cirrhosis of the liver, since this disease is considered to be the main risk factor for the development of the neoplasia. Helicoidal computed tomography, magnetic resonance and/or hepatic arteriography are suggested for diagnostic confirmation and tumor staging. The need to obtain a fragment of the focal lesion for cytology and/or histology for a diagnosis of hepatocellular carcinoma depends on the inability of imaging...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Tumor diagnosis; Tumor staging; Carcinoma therapy; Liver cirrhosis; Barcelona Classification.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100015

BC047440 antisense eukaryotic expression vectors inhibited HepG2 cell proliferation and suppressed xenograft tumorigenicity
Provedor de dados: 56
Autores: Zheng,Lu; Liang,Ping; Zhou,JianBo; Huang,XiaoBing; Wen,Yu; Wang,Zheng; Li,Jing.
The biological functions of the BC047440 gene highly expressed by hepatocellular carcinoma (HCC) are unknown. The objective of this study was to reconstruct antisense eukaryotic expression vectors of the gene for inhibiting HepG2 cell proliferation and suppressing their xenograft tumorigenicity. The full-length BC047440 cDNA was cloned from human primary HCC by RT-PCR. BC047440 gene fragments were ligated with pMD18-T simple vectors and subsequent pcDNA3.1(+) plasmids to construct the recombinant antisense eukaryotic vector pcDNA3.1(+)BC047440AS. The endogenous BC047440 mRNA abundance in target gene-transfected, vector-transfected and naive HepG2 cells was semiquantitatively analyzed by RT-PCR and cell proliferation was measured by the MTT assay. Cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; BC047440; Antisense eukaryotic expression vector; Cell proliferation; Tumorigenicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200002

Polydatin inhibits cell proliferation, invasion and migration, and induces cell apoptosis in hepatocellular carcinoma
Provedor de dados: 56
Autores: Jiao,Yang; Wu,Yan; Du,Dong.
Polydatin, a small molecule from Polygonum cuspidatum, has many biological functions, particularly anti-cancer effects. However, the anti-cancer effects of polydatin in hepatocellular carcinoma (HCC) have not been examined yet. In the present study, MTT assay, BrdU assay, transwell invasion assay, and wound healing assay were performed to determine cell proliferation, invasion and migration. Flow cytometry and TUNEL assay were used to measure cell apoptosis. Quantitative real-time PCR and western blotting assays were used to determine mRNA and protein expression levels. Xenograft experiment was performed to determine the in vivo anti-tumor effect of polydatin. Immunostaining was performed to analyze the expression of caspase-3 and Ki-67. Our results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polydatin; Hepatocellular carcinoma; Proliferation; Apoptosis; Wnt/beta-catenin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400608

Association of MDR1 gene polymorphisms with the risk of hepatocellular carcinoma in the Chinese Han population
Provedor de dados: 56
Autores: Gao,Jian.
The multidrug resistance 1 gene (MDR1) is an important candidate gene for influencing susceptibility to hepatocellular carcinoma (HCC). The objective of the present study was to evaluate the association ofMDR1 polymorphisms with the risk of HCC in the Chinese Han population. A total of 353 HCC patients and 335 healthy subjects were enrolled in the study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), created restriction site-PCR (CRS-PCR) and DNA sequencing methods were used to identify MDR1 gene polymorphisms. Two allelic variants (c.335T&gt;C and c.3073A&gt;C) were detected. The CC genotype of the c.335T&gt;C polymorphism was associated with an increased risk of developing HCC compared to the TT genotype (OR = 2.161, 95%CI...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Multidrug resistance 1 gene; Single nucleotide polymorphisms; Susceptibility; Risk factors.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300311

A poor prognosis in human hepatocellular carcinoma is associated with low expression of DPP4
Provedor de dados: 56
Autores: Yu,Hao; Mei,Xiao-Ping; Su,Peng-Fei; Jin,Guang-Zhi; Zhou,Hong-Kun.
This study aimed to explore the prognostic role of dipeptidyl peptidase 4 (DPP4) expression in hepatocellular carcinoma (HCC). DPP4 expression was measured in formalin-fixed paraffin-embedded specimens that were gathered from 327 HCC patients. Immunohistochemistry analyses were utilized to examine DPP4 expression characteristics and prognostic values (overall survival (OS) and time to recurrence) of DDP4 in HCC tissues. In addition, a patient-derived xenograft (PDX) model was used to assess the correlation between DPP4 expression and tumor growth in vivo. DPP4 was expressed in low levels in HCC tissues in contrast to paired peritumoral tissues (38 cases were down-regulated in a total of 59 cases, 64.4%. P=0.0202). DPP4 expression was significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dipeptidyl peptidase 4; Hepatocellular carcinoma; Immunohistochemistry; X-tile; Overall survival; Patient-derived xenograft.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400609

Aflatoxin M1 in the urine of non-carriers and chronic carriers of hepatitis B virus in Maringa, Brazil
Provedor de dados: 60
Autores: Giolo,Marcel Padovani; Oliveira,Christiane Minervino de; Bertolini,Dennis Armando; Lonardoni,Maria Valdrinez Campana; Gouveia,Matheus Sampaio; Netto,Daisy Pontes; Nixdorf,Suzana Lucy; Machinski Junior,Miguel.
Exposure to aflatoxins (AFs) in the diet may favour the development of hepatocellular carcinoma (HCC) and the acute exacerbation of hepatitis in chronic hepatitis B virus (HBV) carriers. Measurement of biomarkers such as aflatoxin M1 (AFM1), a metabolite of aflatoxin B1 (AFB1), in urine allows for the assessment of populations exposed to aflatoxins. The aim of this study was to investigate the occurrence of aflatoxin M1 in the urine of HBV carrier and non-carrier patients. One group included 43 randomly selected HBV carriers treated at two hospitals in the city of Maringa, Brazil, from March to June 2008. Control group consisted of 29 healthy adult volunteers with anti-HBs positive and HBsAg negative test results. Detection of AFM1 was performed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxins; Biomarkers; Hepatitis B; Hepatocellular carcinoma; Urina/análise toxicológica; Epidemiology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300011

Essential oil from Euphorbia esula inhibits proliferation and induces apoptosis in HepG2 cells via mitochondrial dysfunction
Provedor de dados: 60
Autores: Lv,Dan; Pan,Li-hong; Zhang,Ren; Yang,Jie; Chen,Hao; Wen,Yanzhang; Huang,Mi; Ma,Xinhua; Wang,Qiang; Yang,Xinzhou.
Hepatocellular carcinoma is one of the most prevalent malignancies and a leading cause of cancer-related mortality worldwide. However, the therapies to prevent hepatocellular carcinoma are still limited and the emergence of drug resistance leads to the development of new anti-cancer drugs and combinational chemotherapy regimens. Our study was aimed to explore the anticancer effects of the essential oil extract (EEEO) from Euphorbia esula which has been widely used in traditional Chinese folk medicine and possessed potential cytotoxic effects in several human tumor cells. However, the mechanisms of EEEO-induced anti-proliferation and apoptosis have not been completely elucidated. In this study, EEEO was prepared by hydro-distillation and the main chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbia esula; Hepatocellular carcinoma; Apoptosis; Reactive oxygen species; Mitochondrial pathway.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100506