Consulta: subjectFacets:"Herpesvirus"
Registros recuperados: 31
Data/hora: 04/06/2024 05:45:58

Infections à virus de type herpes chez les bivalves : large spectre d'hôte et détection au stade adulte
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan.
In 1972, Farley reported the presence of herpes-type viral particles in adult American oysters (Crassostrea virginica) for the first time. Since then, this pathogen has been detected in various marine bivalve species, usually associated with massive mortality events. To date, herpes-type viruses have been detected in the following species: C gigas, Ostrea edulis, O. angasi, Tiostrea chilensis, Ruditapes decussatus, R. philippinarum and most recently, Pecten maximus.
Tipo: Text
Palavras-chave: Pathogènes; Bivalves; Herpesvirus; Herpesviridae.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3266.pdf

Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan; Thebault, Anne; Gerard, Andre.
Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of...
Tipo: Text
Palavras-chave: Protein; DNA; Healthy carrier; Adult oyster; Crassostrea gigas; Herpesvirus.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-697.pdf

Shellfish viruses
Provedor de dados: 5
Autores: Renault, Tristan.
Mortalities in a number of commercially important mollusc species have been associated with the detection of viruses belonging to several families. The first description of a virus was in adult eastern oyters, Crassostrea virginica, with the detection of virus particles resembling members of the family Herpesviridae. Subsequently, mass mortalities in French stocks of adult Portuguese oysters, C. angulata, were associated with irido-like virus infection. Other viruses observed in molluscs are described as members of the families Iridoviridae, Papovaviridae and Reoviridae. Little information is available on viral infections that affect molluscs due to primarily to the inadequacy of diagnostic methods that are employed when mass mortality events occur. Most...
Tipo: Text
Palavras-chave: Virus; Scallops; Picornavirus; Oyter herpesvirus 1; Oysters; Molluscs; Irido Like virus; Diseases; Herpesvirus; Clams; Bivalves; Birnavirus; Abalones.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4932.pdf

Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection
Provedor de dados: 5
Autores: Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie.
Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters...
Tipo: Text
Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf

The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA
Provedor de dados: 5
Autores: Chen, Meilin; Kuo, S. T.; Renault, Tristan; Chang, P. H.
A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63 °C and 60 min, respectively. LAMP amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. The method developed was specific for the detection of abalone herpesvirus, without cross-reactions with other tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 100 folds more sensitive than a conventional PCR and 10...
Tipo: Text
Palavras-chave: Herpesvirus; Abalone; Haliotis diversicolor supertexta; Polymerase chain reaction; Loop-mediated isothermal amplification; SYBR green PCR.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00166/27690/25882.pdf

Contribution à l'étude de mollusques marins : Iridovirus-like et Herpèsvirus-like. Description, caractérisation biochimique, cycle de multiplication viral, diagnostic et étude épidémiologique
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Among the marine molluscs, Pacifie oyster, Crassostrea gigas, is actually the most reared specie in the world. Noticely in France, where it was imported between 1960 and 1970, and replaced by Portuguese oyster, Crassostrea angulata, which specie was decimated during this period because of iridoviruses. Uncontroled introductions of new species may however be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases of bivalve molluscs in importing country. In addition to these observations, the quasi monobreeding of Pacific oyster in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this specie, namely the diagnosis of viral diseases. Several families of viruses...
Tipo: Text
Palavras-chave: Mollusques; Crassostrea gigas; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Molluscs; Crassostrea gigas; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00041/15230/12548.pdf

Effects of temperature on herpes-like virus detection among hatchery-reared larval Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Le Deuff, Rose-marie; Renault, Tristan; Gerard, Andre.
This paper describes effects of temperature on herpes-like virus detection in Pacific oyster Crassostrea gigas larvae held at different temperatures. Intranuclear, intracytoplasmic and extracellular viral particles were observed in velum and mantle connective tissues of oyster larvae reared at 25-26°C. In larvae held at 22-23OC, although nuclear lesions were observed, the presence of viral particles was not detected. Results were obtained for oyster larvae with 4 different parental origins. Herpesvirus infection was found in 3 of the 4 groups of oyster larvae held In the same conditions at the higher temperature.
Tipo: Text
Palavras-chave: Temperature; Mortality; Larval culture; Crassotrea gigas; Pacific oyster; Herpesvirus.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/1996/publication-2900.pdf

Evidence for interspecies transmission of oyster herpesvirus in marine bivalves
Provedor de dados: 5
Autores: Arzul, Isabelle; Renault, Tristan; Lipart, Cécile; Davison, Andrew.
Since 1991, numerous herpesvirus infections associated with high mortality have been reported around the world in various marine bivalve species. In order to determine whether these infections are due to ostreid herpesvirus-l (OsHV1), a previously characterized pathogen of the Japanese oyster (Crassostrea gigas), PCR analysis was carried out on 30 samples of larvae collected from four bivalve species (C. gigas, Ostrea edulis, Ruditapes decussatus and Ruditapes philippinarum), most exhibiting mortality prior to collection. All samples were shown to be infected by OsHV1. Viral genomes in three samples of C. gigas and three of R. philippinarum that originated from the same hatchery were unusual in bearing a deletion of at least 2.8 kbp in an inverted repeat...
Tipo: Text
Palavras-chave: Bivalve; Crassostrea gigas; Herpesvirus; Japanese oyster; Transmission.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-705.pdf

Ontogeny and water temperature influences the antiviral response of the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Green, Timothy; Montagnani, Caroline; Benkendorff, Kirsten; Robinson, Nick; Speck, Peter.
Disease is caused by a complex interaction between the pathogen, environment, and the physiological status of the host. Determining how host ontogeny interacts with water temperature to influence the antiviral response of the Pacific oysters, Crassostrea gigas, is a major goal in understanding why juvenile Pacific oysters are dying during summer as a result of the global emergence of a new genotype of the Ostreid herpesvirus, termed OsHV-1 μvar. We measured the effect of temperature (12 vs 22 °C) on the antiviral response of adult and juvenile C. gigas injected with poly I:C. Poly I:C up-regulated the expression of numerous immune genes, including TLR, MyD88, IκB-1, Rel, IRF, MDA5, STING, SOC, PKR, Viperin and Mpeg1. At 22 °C, these immune genes showed...
Tipo: Text
Palavras-chave: Crassostrea; OsHV-1; Water temperature; Antiviral response; Herpesvirus.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00162/27318/25674.pdf

Rapid and sensitive detection of ostreid herpesvirus 1 in oyster samples by real-time PCR
Provedor de dados: 5
Autores: Pepin, Jean-francois; Riou, Antoine; Renault, Tristan.
Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region. The melt curve analysis of OsHV-1 DNA or DNA extracted from infected material showed only one melting temperature peak (75.75 ± 0.1 °C). The assay had a detection...
Tipo: Text
Palavras-chave: SYBR® Green; Real time PCR; Crassostrea gigas; Ostreid herpesvirus 1; Herpesvirus.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3951.pdf

Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review
Provedor de dados: 5
Autores: Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan.
Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1...
Tipo: Text
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf

Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri
Provedor de dados: 5
Autores: Ren, Weicheng; Chen, Haixia; Renault, Tristan; Cai, Yuyong; Bai, Changming; Wang, Chongming; Huang, Jie.
Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV. The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb,...
Tipo: Text
Palavras-chave: Acute viral necrosis virus; Herpesvirus; OsHV-1; Genome; Scallop; Chlamys farreri; AVNV.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00135/24620/22650.pdf

Contribution à l'étude de virus de mollusques marins apparentés aux Iridoviridae et aux Herpesviridae
Provedor de dados: 5
Autores: Le Deuff, Rose-marie.
Among the marine molluscs, Pacific oyster, Crassostrea gigas, is actually the most reared species in the world. Noticeably in France, where it was imported between 1960 and 1970, and replaced Portuguese oyster, Crassostrea angulata. Intensification of breeding and the associated introductions of new species may be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases in bivalve molluscs. In addition to these observations, the quasi monobreeding of C. gigas in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this species, namely the diagnosis of viral diseases. Several families of viruses have been described within marine bivalves molluscs. The purpose of this...
Tipo: Text
Palavras-chave: Mollusques bivalves; Crassostrea gigas; Huître japonaise; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Bivalve molluscs; Crassostrea gigas; Pacific oyster; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00107/21792/19367.pdf

Deciphering the effect of food availability, growth and host condition on disease susceptibility in a marine invertebrate
Provedor de dados: 5
Autores: Pernet, Fabrice; Tamayo, David; Fuhrmann, Marine; Petton, Bruno.
Food provisioning influences disease risk and outcome in animal populations in two ways. On the one hand, unrestricted food supply improves the physiological condition of the host and lowers its susceptibility to infectious disease, reflecting a trade-off between immunity and other fitness-related functions. On the other hand, food scarcity limits the resources available to the pathogen and slows the growth and metabolism of the host on which the pathogen depends to proliferate. Here, we investigated how food availability, growth rate and energetic reserves drive the outcome of a viral disease affecting an ecologically relevant model host, the Pacific oyster, Crassostrea gigas. We selected fast- and slow-growing animals, and we exposed them to high and low...
Tipo: Text
Palavras-chave: Energetics; Health; Herpesvirus; Nutrition; Physiological trade-off.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00513/62431/66716.pdf

Establishing causal link between an infectious agent and mortalities in marine molluscan aquaculture on the example of Bonamia ostreae and Herpèsvirosis in oysters : proposal of a causal grid analysis
Provedor de dados: 5
Autores: Thebault, Anne; Cochennec, Nathalie; Arzul, Isabelle; Renault, Tristan.
In marine molluscan aqauculture, infectious agents are regularly cited in literature associated with mass mortalities as emergin diseases, but the causal link is often based on very few argumeents. 24 ways to demonstrate causal links from epidemiolgical, ecological and marine pathology literature feasible for marine molluscs are given. Those criteria were applied to the ewample of Bonamia ostreae for Ostrea edulis and Herpesvirus for spats and larvae of Crassostrea gigas. Three principles could be applied in marine field for choosing criteria wich should be taken in priority : the demonstration must be given at three levels of organization, organ individual, population, the demonstration must keeps maximum epidemiological criterias, and then mixes...
Tipo: Text
Palavras-chave: Herpesvirus; Bonamia ostrea; Crassostrea gigas; Ostrea edulis; Marine pathology; Ecology; Epidemiology; Infectious agents; Molluscan aquaculture.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3341.pdf

Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae
Provedor de dados: 5
Autores: Batista, Frederico; Taris, Nicolas; Boudry, Pierre; Renault, Tristan.
A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
Tipo: Text
Palavras-chave: DNA extraction; Detection; Larvae; Oyster; OsHV 1; Herpesvirus.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2916.pdf

Infection à virus de type herpès chez les larves de coquilles Saint-Jacques
Provedor de dados: 5
Autores: Arzul, Isabelle; Nicolas, Jean-louis; Renault, Tristan.
King scallop (Pecten maximus) larvae suffered massive mortality during the summer of 2000 at a hatchery located in Brittany. Samples were taken for various types of analyses.
Tipo: Text
Palavras-chave: Pathologie; Pecten maximus; Coquilles St Jacques; Herpesvirus; Herpesviridae.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3267.pdf

French scallops: A new host for ostreid herpesvirus-1
Provedor de dados: 5
Autores: Arzul, Isabelle; Nicolas, Jean-louis; Davison, Andrew J.; Renault, Tristan.
Sporadic high mortalities were reported among larval French scallops (Pecten maximus). Electron microscopy of moribund larvae revealed particles with the characteristics of a herpesvirus in association with cellular lesions. PCR and DNA sequencing showed that the virus is a variant of ostreid herpesvirus-1 that has already been described in clams and oysters. This is the first description of a herpesvirus infection of a scallop species. The virus was transmitted successfully from an extract of infected scallop larvae to uninfected scallop or oyster (Crassostrea, gigas) larvae, demonstrating that it is able to infect both species. Detection of viral DNA in asymptomatic adult scallops by in situ hybridisation indicates that the herpesvirus may have been...
Tipo: Text
Palavras-chave: Bivalve; Host range; Transmission; Polymorphism; OsHV 1; Pecten maximus; Scallop; Herpesvirus.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-739.pdf

Emergence de maladies chez les organismes d'intérêt aquacole : quelques scénarios illustrés d'exemples
Provedor de dados: 5
Autores: Saulnier, Denis; Reynaud, Yann; Arzul, Isabelle; Miossec, Laurence; Le Roux, Frédérique; Goarant, Cyrille.
According to the world organisation for animal health (OIE) an emerging disease is defined as a recently admitted serious illness, whose aetiology can, or not, have already been established, and which is likely to be propagated within a population or between populations, for example at the time of international exchanges of aquatic animals and/or products of aquatic animals. Even though the emerging diseases that affect human health have been much studied, those which affect marine organisms and species of aquaculture interest in particular are poorly documented. By restricting emergence to only infectious diseases, we aim at presenting in a non-exhaustive way some scenarios of the emergence of the diseases of aquacultured species by illustrating them with...
Tipo: Text
Palavras-chave: Cyprinus carpio; Ostrea edulis; Bonamia ostreae; Peneides; Vibrio nigripulchritudo; Herpesvirus; Agent pathogène.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2980.pdf

Recherche d'AND d'herpèsvirus infectant les bivalves dans des échantillons d'eau de claires ostréicoles
Provedor de dados: 5
Autores: Solliec, Gaëlle; Vigneron, Valérie; Montanie, Hélène; Renault, Tristan.
Viral particles, associated with high mortality rates, have been reported among larvae and juveniles in different marine bivalve specie and characterized as member of the Hespesviridae family. Most studies focused on the detection of such viral infections among economically important species. However, the persistence of bivalve herpes viruses in marine environment is poorly documented. This topic appears as an important research domain in order to clarify the natural infection cycle of the herpesvirus of marine bivalves.
Tipo: Text
Palavras-chave: Herpesviridae; Oyster; PCR; DNA; Herpesvirus; Huître; PCR; ADN.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2856.pdf

LATENCIA DEL HERPESVIRUS BOVINO-1: EL PAPEL DE LOS TRANSCRITOS RELACIONADOS CON LATENCIA (RL)
Provedor de dados: 37
Autores: RUIZ,JULIÁN; JAIME,JAIRO; VERA,VÍCTOR.
El herpesvirus bovino-1 es un virus de distribución mundial causante de graves pérdidas económicas debidas principalmente a la disminución de la eficiencia y en los indicadores de salud y productividad de cualquier hato ganadero infectado. Luego de la infección inicial del tracto respiratorio de los animales, el virus establece un estado de latencia viral en las neuronas sensoriales del ganglio trigémino y en los centros germinales de las tonsilas faríngeas. Periódicamente, el virus es reactivado y excretado en secreciones a través de las cuales puede infectar a otros animales susceptibles. Durante dicho estado de latencia hay disminución dramática de la expresión de genes virales, llevando solo a la expresión de dos transcritos: El RNA codificado por el...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herpesvirus; Latencia; Transcritos; ORF.
Ano: 2008
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2008000100001

Tracking viral particles in the intestinal contents of the American bullfrog, Lithobates catesbeianus, by Transmission Electron Microscopy
Provedor de dados: 45
Autores: Antonucci,A.M.; Catroxo,M.H.; Hipolito,M.; Takemoto,R.M.; Melo,N.A.; França,F.M.; Teixeira,P.C.; Ferreira,C.M.
Feces are an important viral agent elimination route for infected carrier animals and in aquatic organisms these pathogenic agents can very rapidly propagate due to the habitation environment. The objective of this work is to track viral particles in the intestinal contents of bullfrogs (Lithobates catesbeianus) from five commercial frog farms in the region of Vale do Paraíba, in the State of São Paulo, Brazil, using negative contrast transmission electron microscopy (TEM). The Coronaviridae, Paramyxoviridae, Parvoviridae and Herpesviridae families were observed and photographed in specimens. This work emphasizes the importance of adopting sanitary measures in commercial farms and confirms that observing feces by TEM is an efficient and rapid diagnostic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphibians; Herpesvirus; Coronavirus; Paramixovirus; Parvovirus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200001

Distribuição de anticorpos para herpesvírus bovino 1 em rebanhos bovinos
Provedor de dados: 45
Autores: Melo,C.B.; Lobato,Z.I.P.; Camargos,M.F.; Souza,G.N.; Martins,N.R.S.; Leite,R.C.
A distribuição de anticorpos neutralizantes para o herpesvirus bovino 1 (HVB 1) foi estudada em quatro faixas etárias de bovinos, em 21 rebanhos de leite e de corte. Os resultados da sorologia foram analisados e relacionados com as respostas de questionários aplicados aos responsáveis pelos rebanhos. As taxas de freqüência de anticorpos neutralizantes para o HVB 1 foram comparadas segundo a aptidão e a faixa etária. Fatores como tipo de manejo e idade dos animais influenciaram na distribuição de anticorpos para o HVB 1.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Herpesvirus; HVB 1; RIB; Anticorpo; Rinotraqueíte.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000600003

Meningoencefalite por Herpesvirus bovino 5 em Minas Gerais: relato de caso clínico
Provedor de dados: 45
Autores: Aquino Neto,H.M.; Carvalho,A.U.; Facury Filho,E.J.; Ferreira,P.M.; Barbosa-Stancioli,E.F.; Lobato,Z.I.P.; Alvarenga,M.R.; Serrano,A.L.; Martins,R.A.; Afonso,D.A.F.
Relata-se um caso de meningoencefalite causada por Herpesvirus bovino 5 (BoHV-5) heritabilityem uma vaca com cinco anos de idade. O animal manifestou quadro clínico inicial de síndrome medular baixa, caracterizada por incoordenação dos membros pélvicos, sinais estes ainda não descritos para a enfermidade. Dentro de pouco tempo a doença evoluiu para síndrome cerebral, e o óbito ocorreu seis dias após o inicio dos sintomas. Na histopatologia, evidenciou-se meningoencefalite difusa, não supurada, e a confirmação do diagnóstico foi feita por reação em cadeia de polimerase e sequenciamento do segmento parcial da glicoproteína G do vírus. O trabalho confirma a presença do BoHV-5 em Minas Gerais, descreve características clínicas novas para a enfermidade e...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Bovino; Herpesvirus; Meningoencefalite; Neuropatia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100001

Visceral Marek's disease in white-peafowl (Pavo cristatus)
Provedor de dados: 45
Autores: Blume,G.R.; Cardoso,S.P.; Oliveira,M.L.B.; Matiolli,M.P.; Gómez,S.Y.M.; Reis Júnior,J.L.; Sant'Ana,F.J.F.; Martins,N.R.S.
ABSTRACT Marek's disease (MD) is a lymphoproliferative disorder caused by Gallid herpesvirus 2 (MDV) that infects mainly domestic gallinaceous birds although wild birds may occasionally be affected. The current report describes the anatomopathological and molecular findings of a case of MD in a white-peafowl (Pavo cristatus). The signs included apathy, hyporexia, and diarrhea. Grossly, 0.5 to 1.5cm in diameter, yellow, soft nodules were observed in the skeletal muscle, lung, kidney, air sacs, small intestine, heart, ovary, ventriculus, and proventriculus. Microscopically, numerous atypical round neoplastic cells were noted. The molecular detection of MDV DNA was implemented to amplify part of the meq gene and products were sequenced for the phylogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marek's disease; Lymphoma; Oncogenic virus; MDV; Herpesvirus.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601602

Use of an ELISA system for detection of equine herpesvirus 1 (EHV-1) antibodies in non-symptomatic pregnant mares and neonatal foals
Provedor de dados: 45
Autores: Carvalho,R.; Passos,L.M.F.; Gouvea,A.M.G.; Resende,M.; Martins,A.S.; Franco,G.C.
An ELISA system was applied to study the prevalence of EHV-1 in vaccinated and non-vaccinated Brazilian horses. Serum samples were taken from non-symptomatic pregnant mares, before and after the parturition, and from their neonatal foals. The antigen was prepared from a purified virus fraction (HVE-1 AB1 strain). The ELISA absorbance of 0.3516 was determined as the threshold of negative values. This cut-off level was defined from a selected interval, which determined a sensibility of 100% and a specificity of 94.7%, from a serum collection obtained from pregnant mares and fetuses. In this study, a strong correlation was found between the ELISA and serum neutralization for specific EHV-1 antibody detection.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Herpesvirus; ELISA.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000300003

Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals
Provedor de dados: 58
Autores: Silva,Débora Scopel e; Castro,Clarissa Caetano de; Silva,Fábio da Silva e; Sant'anna,Voltaire; Vargas,Gilberto D'Avila; Lima,Marcelo de; Fischer,Geferson; Brandelli,Adriano; Motta,Amanda de Souza da; Hübner,Silvia de Oliveira.
P34 is an antimicrobial peptide produced by a Bacillus sp. strain isolated from the intestinal contents of a fish in the Brazilian Amazon basin with reported antibacterial activity. The aim of this work was to evaluate the peptide P34 for its in vitro antiviral properties against canine adenovirus type 2 (CAV-2), canine coronavirus (CCoV), canine distemper virus (CDV), canine parvovirus type 2 (CPV-2), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1). The results showed that the peptide P34 exhibited antiviral activity against EAV and FHV-1. The peptide P34 inhibited the replication of EAV by 99.9% and FHV-1 by 94.4%. Virucidal activity was detected only against EAV. When P34 and EAV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial peptides; Antiviral activity; Herpesvirus; Equine viral arteritis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300043

Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice
Provedor de dados: 60
Autores: Mancini,Dalva Assunção Portari; Torres,Rosângela Pavan; Pinto,José Ricardo; Mancini-Filho,Jorge.
This work aimed to evaluate antiviral properties in antioxidants from spices. Phenolic compounds extracted from rosemary (Rosmarinus officinallis, L) by hot water, had their antioxidant activity determined by spectrophotometry using β carotene/linoleic acid system. The rosemary extract was evaluated by antiviral assay of Herpes Virus type-1 (HSV-1) replication in VERO cells, in the presence or absence of the spice. 10,000 TCID50/mL of the HSV-1 was kept for 3 h at 4º C, with 300 ppm of rosemary extract, and 100 ppm of butyl hydroxyl toluene (BHT). Then, these viruses were inoculated in VERO cells incubated at 37º C in CO2-5 %, for seven days. Daily, they were examined and the end point was based on 100% of CPE in virus control (without antioxidants). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural antioxidants; Virus replication; Phenolic compounds; Herpesvirus; Rosemary.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100016

Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1
Provedor de dados: 65
Autores: Castro,Clarissa Caetano de; Silva,Débora Scopel e; Costa,Géssica Aracéli; Fischer,Geferson; Vargas,Gilberto D’Avila; Brandelli,Adriano; Lima,Marcelo de; Motta,Amanda de Souza da; Hübner,Silvia de Oliveira.
ABSTRACT:  Previous studies have demonstrated the antimicrobial activity of the peptide P34. In this study, the antiviral potential of P34 and the in vitro mechanism of action were investigated against bovine alphaherpesvirus type 1 (BoHV1). P34 exhibited low toxicity, a high selectivity index (22.9) and a percentage of inhibition of up to 100% in MDBK cells. Results from antiviral assays indicated that P34 did not interact with cell receptors, but it was able to inhibit the viral penetration immediately after pre-adsorption. In addition, BoHV1 growth curve in MDBK cells in the presence of P34 revealed a significant reduction in virus titer only 8h post-infection, also suggesting an important role at late stages of the replicative cycle. Virucidal effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiviral; Herpesvirus; Peptide.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600451

Transcriptomic analysis of differential gene expression reveals an increase in COX2 levels during in vitro canine herpesvirus infection
Provedor de dados: 65
Autores: Kurissio,Jacqueline Kazue; Araújo Júnior,João Pessoa.
ABSTRACT: Canine herpesvirus (CaHV-1) affects canids worldwide, causing death in neonates and immunosuppressed hosts. Acute infection by CaHV-1 can cause reproductive, respiratory, and neurological problems in adult animals. Viral pathogenesis and host genes expressions during of CaHV-1infection are not clearly understood. In the present study, the transcriptome of canine kidney cell Mardin-Darby (MDCK) infected in vitro with canine herpesvirus was explored. For this, RNA sequencing (RNA-seq) of the samples in different moments during infection was carried out. Subsequently, the transcriptomic analysis genes related to cell activities and process involved to viral cycle infection were evaluated until 32h post-inoculation (pi). Among evaluated genes, was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transcriptome; Herpesvirus; CaHV-1; PTGS2; COX2; Canine pathogen.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000451

Felis catus gammaherpesvirus 1 (FcaGHV1) and coinfections with feline viral pathogens in domestic cats in Brazil
Provedor de dados: 65
Autores: Kurissio,Jacqueline Kazue; Rodrigues,Marianna Vaz; Taniwaki,Sueli Akemi; Zanutto,Marcelo de Souza; Filoni,Claudia; Galdino,Maicon Vinícius; Araújo Júnior,João Pessoa.
ABSTRACT: Felis catus gammaherpesvirus 1 (FcaGHV1) may causes an asymptomatic infection that result in an efficient transmission and subsequently dissemination of the virus in feline population. This study used molecular detection by qPCR (quantitative PCR) based on DNA polymerase gene fragment amplification to evaluate the occurrence of FcaGHV1 and its correlation with other feline viral pathogens, such as Carnivore protoparvovirus 1 (CPPV-1), Felid alphaherpesvirus 1 (FeHV-1), and feline retroviruses such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). Of the 182 blood samples evaluated 23.6% (43/182) were positives for FcaGHV1. Approximately 37.9% (33/87) of the samples that tested positive for retrovirus were also were positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gammaherpesvirus; Herpesvirus; Feline pathogen; Infectious diseases; Diagnosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300451