Consulta: subjectFacets:"High Perfomance Liquid Chromatography/quantitative analysis."
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Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook
Provedor de dados: 60
Autores: Emir,Ahmet; Emir,Ceren; Bozkurt,Buket; Onur,Mustafa Ali; Somer,Nehir Unver; Kaya,Gulen Irem.
ABSTRACT In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkaloids; Amaryllidaceae; Plant extracts; High Performance Liquid Chromatography/method validation; High Perfomance Liquid Chromatography/quantitative analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100603