Consulta: subjectFacets:"Histochemistry"
Registros recuperados: 58
Data/hora: 16/06/2024 19:17:04

Dynamique et enzymologie de la digestion chez l'huitre Crassostrea gigas
Provedor de dados: 5
Autores: Boucaud, C; Lebesnerais, C; Lubet, P; Lihrmann, I.
Feeding experiments carried out on Crassostrea gigas allowed the authors to set up the stages and the timing of the movement of food throughout the digestive tract. Various activities involved in digestion (glycosidases, esterases, and peptidases) were investigated histochemically in the digestive tract in order to localize the sites of absorption and the sites of protein and carbohydrate digestion. An explicative model of the different stages of digestion is proposed.
Tipo: Text
Palavras-chave: Crassostrea gigas; Bivalvia; Cytology; Histochemistry; Histology; Digestive system; Enzymatic activity; Digestive glands; Digestion; Enzymes; Transit digestif; Digestion; Huitre; Crassostrea gigas.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1179.pdf

Trophic relationships gonad-intestinal loop in Pecten maximus .
Provedor de dados: 5
Autores: Lepennec, M; Dorange, G; Beninger, P; Donval, A; Widowati, I.
Histological, histochemical and ultrastructural studies were performed on the region of the gonad traversed by the intestine in Pecten maximus . The results indicate a digestive and absorptive function of intestine epithelial cells and a transfer of metabolites to the surrounding gonad tissue. Several possible mechanisms of translocation are presented, including diffusion into the haemolymph and transfer via vacuolated cells of the gonad interstitial connective tissue.
Tipo: Text
Palavras-chave: Pecten maximus; Bivalvia; Histology; Haemolymph; Histochemistry; Cells; Metabolites; Epithelia; Intestines; Ultrastructure; Animal reproductive organs.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/acte-1668.pdf

Localisation immunohisto chimique de peptides apparentes aux hormones de vertebres chez Pecten maximus
Provedor de dados: 5
Autores: Donval, A.
Substances related to vertebrate peptidic hormones have been searched by immunohistochemical methods in Pecten maximus . Neurosecretory cells have been detected in the nervous ganglia with antisera raised against vertebrate hormones, gastrin/CCK like, glucagon like, insuline like, somatostatine like. Other gastro-intestinal hormones like are localized in some cells of the gut epithelium: bombesine and vasoactive intestinal peptide.
Tipo: Text
Palavras-chave: Pecten maximus; Bivalvia; Immunology; Histochemistry; Cells; Neurosecretory system; Juveniles; Hormones.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/acte-1724.pdf

Localization of Sugar Residues in the Stomach of Three Species of Monkeys (Tupaiidae glis, Nycticebus cocang and Callithrix jacchus) by Lectin Histochemistry
Provedor de dados: 77
Autores: Fayed,Masoud Hassan; Elnasharty,Mohamed; Shoaib,Mahmoud.
The stomach of three species of non-human primates was investigated by lectin histochemistry to clarify the staining affinity and distribution patterns of their sugar residues. All gastric regions, with little differences between the deep and superficial parts of the same region, were rich in. in N-acetylglucosamine and/or neuraminic acid. Although, the superficial regions of the gastric mucosa were scanty in N-acetylgalactosamine, a- D-glucose and a -D-mannose, the deep parts of the gastric mucosa were rich in these sugars. In conclusion, there is a difference among the mucosubstances of surface and foveolar mucous cells, mucous neck cells, and gastric gland cells. This indicates heterogeneous composition of gastric mucus, or mucus molecules with...
Tipo: Journal article
Palavras-chave: Histochemistry; Lectins; Non-human primates; Stomach.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100016

Clinical and Histochemical Alterations of the Periodontal Ligament in Gerbils after Malocclusion Induced
Provedor de dados: 77
Autores: Naves,Leandro Moura Leite; Issa,João Paulo Mardegan; Pitol,Dimitrius Leonardo; Fukada,Sandra Yasuyo; di Matteo,Miguel Ángel Sala; Iyomasa,Mamie Mizusaki.
The aim of this article is to show the clinical and histochemical alterations of the first periodontal ligament, on the right side, after upper molars teeth extraction on the left side in gerbils. After two months, the periodontal ligaments were removed and processed for histochemical analysis. The data showed that TRAP reaction was able to evidence the osteoclastic activity in the hyperfunction hemimandible, right side, explaining the functional changes in the periodontal ligament after teeth extraction, and a little gingival recession and radicular exposure of teeth without function was observed at inferior molars of the left side
Tipo: Journal article
Palavras-chave: Periodontal ligament; Histochemistry; Malocclusion; Gerbil; Meriones unguiculatus.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400036

Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion
Provedor de dados: 77
Autores: Chagas,M. A.; Babinski,M. A.; Abidu-Figueiredo,M.; Costa,W. S.
The otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ANS). Similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. We have studied rat otic ganglion in 10 animals through light microscopy. After routine histological methods, 5 µm sections were obtained and coloured by Gomori`s trichrome, periodic acid-Schiff (PAS), Alcian Blue pH 2.5 and pH1.0, acetylation + PAS, acetylation + deacetylation + PAS, acid hydrolysis + PAS, phenylhydrazine + PAS and thiosemicarbazide +...
Tipo: Journal article
Palavras-chave: Rat; Otic ganglion; Autonomic nervous system; Histochemistry.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000100015

Histological and Histochemical Changes by Clove Essential Oil Upon the Gonads of Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae)
Provedor de dados: 77
Autores: Cruz,Glaucilane dos Santos; Teixeira,Valeria Wanderley; de Oliveira,Jose Vargas; Teixeira,Alvaro Aguiar Coelho; Araújo,Alicely Correia; Alves,Thiago Jose de Souza; da Cunha,Franklin Magliano; Breda,Mariana Oliveira.
Spodoptera frugiperda is a polyphagous insect that causes economic losses to several crops in Brazil and is the major obstacle to corn production. Researches focusing on alternative control, e.g. botanical products are expanding to offer a wide variety of molecules that interfere with different biological parameters of insect pests. Thus, this study tested the hypothesis that clove essential oil affects the spermatogenesis, ovarioles histochemistry and the fertility of S. frugiperda. The results showed that clove essential oil affects the gametogenesis of S. frugiperda ovarioles, reflecting negatively on its reproduction, proving to be a promising tool for controlling this pest.
Tipo: Journal article
Palavras-chave: Fall armyworm; Spodoptera frugiperda; Reproduction; Histochemistry; Histology; Syzygium aromaticum.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400034

Influence of Reduced Salinity on the Histochemical Fiber Type Composition of the Claw Closer Muscle of Two Estuarine Crab Species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae)
Provedor de dados: 77
Autores: Longo,María Victoria; Díaz,Alcira Ofelia.
The estuarine crustaceans are exposed to frequent and abrupt environmental salinity changes that involve adjustments at different levels in muscle tissue. The histochemical composition of fiber types of the claw closer muscle of Cyrtograpsus angulatus and Neohelice granulata (Dana, 1851) under hyper regulatory conditions was analyzed. Cryosections of the muscle were treated with myosin-adenosine triphosphatase (m-ATPase), succinic dehydrogenase (SDH), periodic acid Schiff (PAS) and Sudan Black B. The mean diameters, the relative area and the proportion of each muscle fiber type were calculated. Types I and IV would belong to 'extreme' groups, whereas types II and III would be considered 'intermediate'. Type I fibers were large and exhibited a weak reaction...
Tipo: Journal article
Palavras-chave: Euryhaline crabs; Histochemistry; Hyper regulation; Muscle fibers; Salinity stress.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200025

Lymphoglandular Complexes in Proximal Colon of Buffalo Calves (Bubalus bubalis)
Provedor de dados: 77
Autores: Kapoor,Kritima; Singh,Opinder.
The present study was conducted on six healthy early neonatal and six prepubertal buffalo calves to study the location, gross morphology, histomorphology and histochemistry of lymphoglandular complexes in proximal colon. In very proximal part of colon of buffalo calves, an irregular oval mucosal lymphoid patch was found grossly as a proximal colon (PC) patch. Histologically, in proximal colon patch of early neonates (3-4 weeks), an extensive invasion of mucosal glands was observed towards lymphoid nodules that were present in submucosa. The structure as a whole thus formed a complex known as lymphoglandular complex (LGC). Large number of such complexes i.e., LGCs were observed in submucosa of proximal colon at this age. At some places, invasion of mucosal...
Tipo: Journal article
Palavras-chave: Proximal colon; Lymphoglandular complex; Histomorphology; Histochemistry; Buffalo calves.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300052

Histological and Histochemical Studies of the Aorta and Pulmonary Trunk in STZ-induced Diabetic Wistar Rats Treated with Momordica charantia
Provedor de dados: 77
Autores: Komolafe,O. A; Ofusori,D. A; Adewole,O. S; Ayoka,A. O; Bejide,R.
Diabetes mellitus (DM) is a serious metabolic disorder with micro and macro-vascular complications that result in a significant morbidity and mortality. The present study investigated the effects of Momordica charantia (M. charantia) on histological changes of the aorta and pulmonary trunk in streptozotocin-induced diabetic Wistar rats. Forty healthy adult Wistar rats of both sexes were randomly assigned into five groups A, B, C, D and E of eight rats each. Group A were the control (normal rats); B were the experimentally-induced diabetic rats; C were diabetic rats treated with methanolic extracts of M. charantia for two weeks (withdrawal group); D were diabetic rats treated with methanolic extracts of M. charantia for four weeks. E was diabetic rats...
Tipo: Journal article
Palavras-chave: Diabetes; Momordica charantia; Histological; Histochemistry; Aorta; Pulmonary trunk.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200060

Age Related Structural Changes in the Bulbourethral Gland of Gaddi Goat (Capra hircus)
Provedor de dados: 77
Autores: Archana,P; Katiyar,R. S; Sharma,D. N; Farooqui,M. M; Prakash,Ajay.
A study was conducted on the bulbourethral glands of 30 Gaddi goats, divided into three groups of 10 animals in each viz; Prepubertal (1 day old to &lt; 18 months of age), Pubertal (18 months to &lt; 5yrs of age) and Postpubertal (&gt;5yrs of age). The study revealed that the glands grew at a faster speed up-to 6 months of postnatal life after which the pace slowed down considerably. Almost 10 times increase in weight and more than three times increase in all the linear mensurations were observed in prepubertal group from the time of birth. The stroma vs parenchyma ratio which was 80:20 in one-day-old kids showed three times increase in parenchyma in prepubertal animals, and the ratio became 43:57. In one day old kids luminated and non-luminated secretory...
Tipo: Journal article
Palavras-chave: Bulbourethral gland; Gaddi goat; Histology; Histochemistry.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200049

Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions
Provedor de dados: 77
Autores: Rosero-Salazar,Doris Haydee.
Different situations cause ischemia and reperfusion injury, affecting tissues under the level of compression. In this research, abnormal characteristics in distribution of muscle fibers types in soleus and extensor carpi radialis longus, during short periods of ischemia and short and long periods of reperfusion, were determined. Fibers were classified by enzyme histochemistry techniques NADH-TR and myosin-ATPase. Measurements of areas were carried out through semiautomatic image processing by using segmentation based on regions, which evidenced significant changes in distribution during reperfusion followed to one and three hours of ischemia, as well as in comparisons of areas for all periods of reperfusion. This study strengthens the evidence about using...
Tipo: Journal article
Palavras-chave: Histochemistry; Ischemia; Reperfusion; Image analysis; Segmentation; Histoquímica; Isquemia; Reperfusión; Análisis de imágenes; Segmentación.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100019

Mucous Membrane of the Rumen of Ovines, Fed With Spineless, Forrage Cactus or Palm (Barbary Fig) (Opuntia ficus indica Mil): Hystochemical Study by Means of Light Microscopy
Provedor de dados: 77
Autores: Neiva,Gentileza Santos Martins; da Mota,Diógenes Luís; Batista,Ângela Maria Vieira; Sousa-Rodrigues,Célio Fernando de.
The mucosa of the rumen performs the functions of absorption, transportation, metabolism and protection, due to the presence of microorganisms in this compartment of the digestive tract, which are responsible for the digestion of approximately 60% of the organic material apparently digested. The histological structure and the histochemical profile of glicoconjugate of the mucosa of the stomach of sheep that were being fed with forrage cactus or palm (Giant Palm - Opuntia ficus indica Mil)1. Twelve sheep were used, with approximately 14 months old, which were oddly distributed in four diets: D1- 60.0% forrage palm + 40.0% concentrated ration; D2- 47.5% forrage palm + 12.5% elephant, napier grass + 40.0% concentrated ration, and D3 - 34,8% forrage palm +...
Tipo: Journal article
Palavras-chave: Mucous membrane; Histology; Histochemistry; Forrage palm; Forrage cactus; Ovine.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500034

Preliminary Study of the Sonic Muscle of Micropogonias furnieri (Actinopterygii, Sciaenidae): Morfología e Histoquímica
Provedor de dados: 77
Autores: Devincenti,Clelia Viviana; Díaz,Alcira Ofelia; Longo,María Victoria; García,Alicia Mabel; Figueroa,Daniel; Goldemberg,Adriana Lía.
The present study highlights the morphology of the Micropogonias furnieri sonic muscle by means of histochemical techniques, relating it to previous histochemical studies of the pectoral fin and myotomal muscles. In order to classify the muscle fibres, succinic dehydrogenase (SDH) for mitochondria, periodic acid Schiff (PAS) for glycogen, Sudan Black and Red for lipids and myosin adenosine triphosphatase (m-ATPase) pre-incubated at alkaline and acid pHs to visualize the contraction velocity, were used. The sonic fibres were smaller than the white myotomal and pectoral fibres, showed homogenous size and distribution and had features common to white fibres: negative to SDH and lipids, weakly positive to PAS and m-ATPase following acid pre-incubation, and...
Tipo: Journal article
Palavras-chave: Sonic muscle; Micropogonias furnieri; Morphology; Histochemistry.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400027

Morphometry and Histochemistry of the Rectus abdominis Muscle Fibers of Tufted Capuchin Monkeys (Cebus apella Linnaeus, 1758)
Provedor de dados: 77
Autores: Simionato,L. H.; Andreo,J. C.; Oliveira,J. A. de; Bortoluci,C. H. F.; dos Santos,N. B.; Moraes,L. H. R.; Rodrigues,A. C.; Andreo,M. B.
The phylogenetic proximity of primates to humans, along with their behavioral, biochemical, and anatomical similarities, make such animals more interesting experimental models for biomedical researches, as compared to classical laboratory animals. Another aspect that has called the attention of researchers is the differentiated quadrupedalism present in some primates. The tufted capuchin monkey uses the ground and tree branches as its support for locomotion, showing various postures while performing this task. On the basis of this information, we have decided to study the rectus abdominis muscle of the tufted capuchin monkey, with the following goals: the frequency and area of fiber types; its possible compartmentalization; and identify if this muscle is...
Tipo: Journal article
Palavras-chave: Rectus abdominis muscle; Histochemistry; Musculars fiber types; Tufted capuchin monkey; Cebus apella; Primates; Simians.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000100011

Comparative Intestinal Histomorphology of Five Species of Phyllostomid Bats (Phyllostomidae, Microchiroptera): Ecomorphological Relations with Alimentary Habits
Provedor de dados: 77
Autores: Gadelha-Alves,Rafaela; Rozensztranch,Andréa Melo Da Silva; Rocha- Barbosa,Oscar.
The intestines of Sturnira lilium, Phyllostomus hastatus, Carolliaperspicillata, GlossophagasoricinaíindDesmodus rotundus were analized through macroscopic, morphometric and light microscopy studies. The species studied presented some common charactenstics generally found in bats, such as the absence of a caecum and an appendix. The frugivorous bat S. lilium has a longer intestine than the other species. The intestinal epithelium is of the simple columnar type, constituted of absorptive cells characterized by microvilli and goblet cells, which in all the species proved to be PAS + and AB +. These cells presented some differences in quantity and in distribution among the species. The intestinal mucosa presented morphologic and morphometric differences, such...
Tipo: Journal article
Palavras-chave: Intestines; Bats; Phyllostomidae; Histology; Histochemistry; Micrometry; Ecomorphology.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000300014

Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat
Provedor de dados: 77
Autores: Núñez Chichet,M E; Genovese,P; Bielli,A.
The vomeronasal organ (VNO) is a tubular chemoreceptory organ which detects environmental pheromones and is essential for mammalian normal reproductive activity. A sensitive chemoreceptory neuroepithelium lines the concave VNO wall and the opposite, convex wall is lined by a seudostratified, receptor-free epithelium. The secretion of the Jacobson glands (JG) -tubuloacinary glands lying in the lamina propia of the VNO - is essential for pheromones contacting vomeronasal organ chemoreceptors. Tubuloacinary glands lying in the connective tissue of the nasal septum mucosa (Bowman glands), can be classified as ventral and dorsal, according to their location. Positivity to PAS and Alcyan blue reactions and androgen receptor immunolocalization was evaluated with...
Tipo: Journal article
Palavras-chave: Vomeronasal organ; Chemoreception; Development; Histochemistry; Immunohistochemistry.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300018

Histopathology of Organs from Neonate Offspring Born to Female Rats Exposed to Sublethal Doses of Biological and Synthetic Insecticides
Provedor de dados: 77
Autores: dos Santos,Hilda Michelly Paiva; Teixeira,Valéria Wanderley; Teixeira,Álvaro Aguiar Coelho; da Silva,Rosimere; Lemos,Ana Janaina Jeanine Martins; Neto,Clovis José Cavalcanti Lapa; Ferreira,Cintia Giselle Martins.
The current annual consumption of insecticides in agriculture in Brazil is over three million tons. The exposure happens through food that is contaminated with toxic waste and respiratory and dermal absorption. Biological control with Bacillus thuringiensis Berliner (Bt) has assumed increasing importance replacing the use of conventional insecticides. However, the tests used to justify approval for use and market release of Bt products, and the protocols used to assess the development of toxicity, are extremely superficial. In addition, more studies comparing effects between males and females and during pregnancy and lactation have not been conducted. Thus, the hypothesis tested in this study was if the administration of the XenTari® WG (Bacillus...
Tipo: Journal article
Palavras-chave: Bacillus thuringiensis; Synthetic insecticide; Rats; Histology; Histochemistry.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100040

Histology and histochemistry of the venom apparatus of the black scorpion Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae)
Provedor de dados: 80
Autores: Jarrar,BM; Al-Rowaily,MA.
The venom apparatus of the black scorpion Androctonus crassicauda has been characterized histologically and histochemically in the present study. The results showed that this apparatus consists of paired venom glands, each of which initially presents its own canal and posteriorily both fuse into a single common one. Each gland is covered by a sheath of striated muscle and is lined with extensively folded secretory epithelium (formed of non-secretory and secretory venom-producing cells). The outcomes also revealed that the venom-producing cells of both glands produce neutral mucosubstances, sialomucins, sulfomucins and proteins, but are devoid of glycogen. Cysteine, tyrosine, tryptophan and arginine were also detected along with activities of acid and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Androctonus; Scorpion; Venom apparatus; Histochemistry; Phosphatases; Esterases; Peptidases.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300010

Ultramorphological analysis of the venom glands and their histochemical relationship with the convoluted glands in the primitive social paper wasp Polistes versicolor (Hymenoptera: Vespidae)
Provedor de dados: 80
Autores: Britto,F. B.; Caetano,F. H.
The venom glands are part of the most important defense weapon in Aculeata: the venom apparatus. The arrangement of these glands can vary among species, but in general they are composed of long secretory tubules connected to a muscular sac-like reservoir. Although the occurrence of these variations has been documented, many studies neglected the existence of a well-developed secretory portion in the lumen of the reservoir named convoluted gland. This study is an ultramorphological analysis of the venom glands and their histochemical relationship with the convoluted glands in the primitive social wasp Polistes versicolor. In this wasp, the venom glands are constituted by two tubular portions that penetrate individually in the venom reservoir, inside of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wasps; Venom glands; Convoluted glands; Ultramorphology; Histochemistry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200007

Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae
Provedor de dados: 82
Autores: Pinheiro,Daniela O.; Quagio-Grassiotto,Irani; Gregório,Elisa A.
The sugarcane borer, Diatraea saccharalis Fabricius, is a pest to sugarcane and many other crops. This work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of D. saccharalis larvae. Fragments of the midgut (anterior, middle and posterior regions) were fixed and processed by light and scanning electron microscopy. There are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. The apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions. There is an increase in the amount of PAS-positive (Periodic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphology; Sugarcane borer; Histochemistry; Scanning electron microscopy; Light microscopy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000400009

Development and some histochemical aspects of foliar glandular trichomes of Stevia rebaudiana (Bert.) Bert. - Asteraceae
Provedor de dados: 88
Autores: MONTEIRO,WALKYRIA ROSSI; CASTRO,MARÍLIA DE MORAES; MAZZONI-VIVEIROS,SOLANGE CRISTINA; MAHLBERG,PAUL GORDON.
The ten-celled biseriate glandular trichome of Stevia rebaudiana (Bert.) Bert.-Asteraceae, found on both leaf surfaces, originates from a single protruding, protodermal cell undergoing an anticlinal division. A subsequent series of periclinal divisions, occurring in acropetal sequence, leads to the formation of the trichome, composed of five pairs of cells, one pair of basal cells, another of stalk cells and three pairs of secretory head cells. Developing, still two-celled glandular trichomes already occur on leaf primordia of the second pair (these primordia measuring, in some cases, ca. 0.30 mm in length), and most of the glandular trichomes are at the mature phase on very young, expanding leaves, for example on those of the sixth pair. The secretory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stevia rebaudiana; Asteraceae; Glandular trichomes; Ontogeny; Histochemistry.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000300013

Oil glands in the Neotropical genus Dahlstedtia Malme (Leguminosae, Papilionoideae, Millettieae)
Provedor de dados: 88
Autores: Teixeira,Simone de Pádua; Rocha,Joecildo Francisco.
Dahlstedtia pentaphylla (Taub.) Burkart and D. pinnata (Benth.) Malme belong to the Millettieae tribe and are tropical leguminous trees that produce a strong and unpleasant odour. In the present work, we investigated the distribution, development and histochemistry of foliar and floral secretory cavities that could potentially be related to this odour. The ultrastructure of foliar secretory cavities were also studied and compared with histochemical data. These data were compared with observations recorded for other species of Millettieae in order to gain a phylogenetic and taxonomic perspective. Foliar secretory cavities were only recorded for D. pentaphylla. Floral secretory cavities were present in the calyx, wings and keels in both species; in D....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Development; Histochemistry; Secretory cavity; Ultrastructure.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000100006

Anatomy, ultrastructure and secretion of Hibiscus pernambucensis Arruda (Malvaceae) extrafloral nectary
Provedor de dados: 88
Autores: Rocha,Joecildo Francisco; Machado,Silvia Rodrigues.
This paper reports on the extrafloral nectary (EFN) of Hibiscus pernambucensis, a native shrub species occurring in mangrove and restinga along Brazil's coastline. EFNs occur as furrows with a protuberant border on the abaxial surface veins of the leaf blade. Each nectary consists of numerous secretory multicellular trichomes, epidermal cells in palisade-like arrangements and non-vascularized parenchyma tissue. Nectar secretion is prolonged, since secretion starts in very young leaves and remains up to completely expanded leaves. Reduced sugars, lipids, and proteins were histochemically detected in all the nectary cells; phenolic substances were detected in the vacuoles of the epidermal palisade cells and in some secretory trichome cells. The secretory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Extrafloral nectary; Hibiscus pernambucensis; Histochemistry; Ultrastructure.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000300008

Structural Differences of the Harderian Gland between Common Pheasants (Phasianus Colchicus Talischensis) and Hybrids of Italian Amber and Common Pheasants
Provedor de dados: 89
Autores: Klećkowska-Nawrot,J; Goździewska-Harłajczuk,K; Kowalczyk,A; Łukaszewicz,E; Nowaczyk,R.
ABSTRACT The aim of the present study was to demonstrate the histological and histochemical structure of the Harderian gland in wild and hybrid of wild and domestic birds. The samples were stained with haematoxylin-eosin, methyl green-pyronin Y, periodic acid-Schiff, alcian blue pH 2.5, aldehyde fuchsin and Hale's dialyzed iron staining's. In both species, the glands had multilobar tubuloacinar structure type I. The Harderian gland was located in the orbit near the inter-orbital septum, between the medial rectus muscle, the pyramidal muscle of the third eyelid, and the ventral oblique muscle. In the common pheasant, the gland was wider in the proximal and distal part. The common pheasant had more elongated lobes of the Harderian gland than in the hybrid....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Harderian gland; Histology; Histochemistry; Common pheasant; Hybrid of Italian amber and common pheasant.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200309

MORPHOANATOMY OF Garcinia madruno (KUNTH) HAMMEL (CLUSIACEAE) UNDER WATERLOGGED CONDITIONS
Provedor de dados: 94
Autores: ABREU,NATÁLIA DO COUTO; BARBOSA,SUZANA MARQUES; GURGEL,ELY SIMONE CAJUEIRO; CARVALHO,WENDELL VILHENA DE.
ABSTRACT Garcinia madruno (Kunth) Hammel is a neotropical tree that naturally occurs in terra firme forests and is important as a source of income and medicine for Amazonian populations. This study describes and compares the morphoanatomical responses of plants under conditions of stress and normoxia. Young plants of Garcinia madruno were subjected to two water regimes: daily controlled irrigation and waterlogged for 84 days. Hypertrophic lenticels, adventitious roots and anatomical characteristics of the roots and leaves were evaluated on days 0, 21, 42, 63 and 84. There were no changes in leaf structure, but cracks and hypertrophic lenticels appeared on the stems, there was an increase in blackness of the roots and the plants formed adventitious roots to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon; Bacurizinho; Stress; Fruit; Histochemistry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000500201

Comparative analysis of the integument of different tree frog species from Ololygon and Scinax genera (Anura: Hylidae)
Provedor de dados: 103
Autores: Silva,Henrique Alencar Meira da; Silva-Soares,Thiago; Brito-Gitirana,Lycia de.
ABSTRACT The integuments of ten treefrog species of two genera from Scinaxnae - O. angrensis (Lutz, 1973), O. flavoguttata (Lutz &amp; Lutz, 1939), O. humilis (Lutz &amp; Lutz, 1954), O. perpusilla (Lutz &amp; Lutz, 1939), O. v-signata (Lutz, 1968), Scinax hayii (Barbour, 1909), S. similis (Cochran, 1952), O. trapicheroi (Lutz &amp; Lutz, 1954) and S. x-signatus (Spix, 1824) - were investigated using conventional and histochemical techniques of light microscopy, and polarized light microscopy. All integuments showed the basic structure of the anuran integument. Moreover, the secretory portions of exocrine glands, such as serous merocrine and apocrine glands, were found to be restricted to the spongious dermis. Lipid content occurred together with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian Atlantic forest; Histochemistry; Hylids; Treefrog.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702017000100327

The sexual dimorphic inguinal glands of the frog species Ololygon centralis (Anura: Hylidae) at light and transmission electron microscopy
Provedor de dados: 103
Autores: Brito,Pedro; Targueta,Cíntia P.; Arruda,Walquíria; Santos,Fernanda; Bastos,Rogério.
ABSTRACT The anuran skin characteristically has different types of glands, most of which are microscopic and are spread throughout the skin. Some species have specialized regions where glands agglomerate, forming macroglands. The description of the external morphology of Ololygon centralis (Pombal &amp; Bastos, 1996) revealed the presence of an inguinal gland. Ololygon centralis is the only species of the genus that has a macrogland. The present study found these inguinal macroglands to be present only on male specimens, thus characterizing it as a sexually dimorphic skin gland. Microscopic analysis revealed that these glands are composed of many syncytial units involved by myoepithelial cells. The center of the syncytium is full of a proteinaceous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histochemistry; Morphology; Skin; Serous gland.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702019000100317

The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study
Provedor de dados: 103
Autores: Hernández,María Sol; Longo,María Victoria; Devincenti,Clelia Viviana; Díaz,Alcira Ofelia.
ABSTRACT The adductor pectoral fin muscle in post-spawning females of the white croaker Micropogonias furnieri (Desmarest, 1823) is described from a morphological, histochemical and morphometric perspectives. A description of the morphological characteristics was conducted after dissections in different layers, down to the deep layer. Five muscles were found: superficial, medial, radial and deep adductors, and dorsal arrector. Their fibers were studied after applying histochemical techniques: succinic dehydrogenase (SDH) to reveal mitochondria, periodic acid-Schiff (PAS) for glycogen, Sudan black for lipids, myosin adenosinetriphosphatase (mATPase) to reveal the types of fibers, and modified mATPase to evidence the capillaries. Fiber diameters were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fin musculature; Histochemistry; Morphology; Morphometry; White croaker.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702016000600902

Histochemical aspects of wheat resistance to leaf blast mediated by silicon
Provedor de dados: 63
Autores: Silva,Washington Luís da; Cruz,Maria Fernanda Antunes; Fortunato,Alessandro Antônio; Rodrigues,Fabrício Ávila.
Blast, caused by Pyricularia oryzae, has become a significant disease threat to wheat (Triticum aestivum L.) in Brazil. This study aimed to investigate at the histochemical level if silicon (Si) could enhance the production of flavonoids in the leaves of wheat plants in response to P. oryzae infection. Plants from the Aliança cultivar, which are susceptible to blast, were grown in hydroponic cultures containing 0 (-Si) or 2 mM of Si (+Si) and inoculated by spraying a conidial suspension of P. oryzae (1 × 105 conidia mL−1) on all adaxial leaf surfaces of plants at 60 days after emergence (growth stage 65). The fourth and fifth leaves of each plant were used to evaluate blast severity at 24, 36, 48, 72 and 96 h after inoculation (hai). At 96 hai, leaves were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyricularia oryzae; Flavonoids; Histochemistry; Phenolics.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000400322

Aspectos reprodutivos de Sphoeroides greeleyi (Gilbert), Pisces, Osteichthyes, Tetraodontidae, da gamboa do Baguaçu, Baia De Paranaguá, Paraná, Brasil
Provedor de dados: 104
Autores: Schultz,Yart Damasceno; Favaro,Luís Fernando; Spach,Henry Louis.
The present study analyses the reproductive aspects of Sphoeroides greeleyi (Gilbert, 1900), such as morphology and histochemistry of carbohydrates in the oogenesis phases, maturity scale, time and type of spawn, sexual rate and first ripened mature size for females. From the monthly captured animais (Nov 1998 to Nov 1999) the morphometric data (entire length and total weight) were extracted and the sex and the gonadal maturity were determined. Some ovaries were prepared for microscopic analysis that charac-terized six phases for the development of the ovarian folheies. Through the carbohydrates analysis the acid carbohydrates could be related to the oogenesis maintenance and the neutral carbohydrates to the beginning of embryonic development. A maturity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Reproduetion; Histology; Histochemistry.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752002000100004

Aspectos histológicos e histoquímicos da cloaca feminina de Columba livia (Gmelin) (Columbidae, Columbiformes)
Provedor de dados: 104
Autores: Teles,Maria Eloíza de Oliveira; Ribeiro,Maria das Graças; Maruch,Sandra Maria das Graças; Ribeiro,Roseli Deolinda.
The domestic dove Columba livia (Gmelin, 1789) is a species well adapted to Brazil and the study of its reproductive biology is part of a broad research project on birds. This essay describes the morphological aspects of the cloaca of female Columba livia, describing compartments lengthwise starting from the head such as the coprodeo, urodeo and proctodeo limited by mucosal folds. Each compartment of the cloaca presents its own morphological characteristics which differ one from the other in form, height and position of mucosal projections, kinds of tissues, presence and histological aspects of glands, presence of lymphonodus. The rectum opens into the coprodeo, the ureter opens into the oviduct and the cloacal sac opening into the proctodeo. Histochemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Domestic dove; Morphological aspects; Histochemistry; Cloaca.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000100013

Morphoanatomy of nectaries of Chamaecrista (L.) Moench sections Chamaecrista , Caliciopsis and Xerocalyx (Leguminosae: Caesalpinioideae)
Provedor de dados: 39
Autores: Silva,Marinalva dos Santos; Coutinho,Ítalo Antônio Cotta; Araújo,Maicon Nascimento; Meira,Renata Maria Strozi Alves.
ABSTRACT Nectaries are specialized structures that secrete nectar. Several species of Chamaecrista possess nectaries on the petiole, which have been shown to vary widely in morphology and the chemical nature of their secretion. However, a comprehensive investigation of the nectaries of the clade formed by sect. Chamaecrista, Caliciopsis and Xerocalyx has yet to be performed. Our study aimed to confirm whether or not the leaf glands of species of this clade are in fact nectaries, determine the chemical nature of their secretion and expand the morphoanatomical database on leaf nectaries in Chamaecrista with the intention of contributing to the taxonomy and phylogeny of the genus. Samples from herbarium and field-collected material were subjected to standard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Foliar glands; Histochemistry; Leaf glands; Secretory structures; Taxonomy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300445

Extrafloral nectaries of annatto ( Bixa orellana L.): anatomy, nectar composition and activity during organ development
Provedor de dados: 39
Autores: Miranda,Rafaela Marques de; Nery,Lays Araújo; Ventrella,Marília Contin.
ABSTRACT This study aimed to anatomically characterize the extrafloral nectaries (EFNs) of annatto (Bixa orellana) and determine the composition of its nectar in order to better understand their structure and function during organ development. Standard light and scanning electron microscopy techniques were used for anatomical analysis, and test-strips and a refractometer for determining nectar composition. Both receptacle and stem EFNs were found to possess similar anatomy and nectar composition, and to secrete from early to advanced developmental stages of the organs to which they are associated. EFNs consisting of uniseriate epidermis, nectariferous parenchyma and subnectariferous parenchyma were found located where vascular tissue is immersed. Some...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ants; Histochemistry; Nectar release; Secretory structure; Urucum.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300468

Does an urban environment affect leaf structure of Eugenia uniflora L. (Myrtaceae)?
Provedor de dados: 39
Autores: Bezerra,Laís de Almeida; Callado,Cátia Henriques; Cunha,Maura Da.
ABSTRACT External factors can interfere with the structure and biological activity of plants. Nevertheless, the susceptibility of plants to specific environmental conditions varies, which raises many questions about the behavior of medicinal plants when grown in urban areas. This study aims to detect possible changes induced by exposure of Eugenia uniflora L. to an urban environment, with emphasis on variation in external and internal leaf structure and differences in the production of its main metabolites. We compared leaves of E. uniflora cultivated in forest and urban sites and analyzed them for structural plasticity and characteristics indicative of stress in the urban environment. The leaves of the urban site revealed necrosis and reddish spots,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atmospheric pollution; Brazilian cherry; Environmental change; Histochemistry; Pitangueira; Urban environment.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000200266

Leaf glands of Banisteriopsis muricata (Malpighiaceae): distribution, secretion composition, anatomy and relationship to visitors
Provedor de dados: 39
Autores: Nery,Lays Araújo; Vieira,Milene Faria; Ventrella,Marília Contin.
ABSTRACT Leaf glands are common structures in Malpighiaceae and exhibit great morphological diversity, yet information on their anatomy, secretion and type of visitors remains scarce. The aim of this study was to describe the distribution, anatomical development and chemical and functional properties of leaf glands of Banisteriopsis muricata (Malpighiaceae). Leaves at different stages of development were collected and processed according to standard techniques for light and scanning electron microscopy. Secretion composition was determined by histochemical tests and test-strips, while gland funciton was determined by field observation of interactions with visitors. Leaf glands were located on the petiole and on the abaxial base of the leaf blade. The gland...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Ants; Extrafloral nectaries (EFNs); Histochemistry; Leaf glands; Nectar; Ontogeny.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300459

Comparative anatomy of calyx and foliar glands of Banisteriopsis C. B. Rob. (Malpighiaceae)
Provedor de dados: 39
Autores: Araújo,Josiane Silva; Meira,Renata Maria Strozi Alves.
Banisteriopsis is considered one of the largest genera of Malpighiaceae with 58 species, of which 47 occur in Brazil. The typical calyx and leaf glands of Banisteriopsis are considered relevant to the adaptive success of Malpighiaceae. Comparative studies of anatomical and histochemical characteristics may reveal similarities and assist in the interpretation of the functions performed by such glands. The present study aimed to describe the anatomy of the calyx and leaf glands of 38 species ofBanisteriopsis that occur in Brazil, and to analyze these structures histochemically in B. campestris, B. laevifolia and B malifolia, using standard methods. Calyx glands differ from leaf glands by possessing an irregular surface that is covered by a thick cuticle that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Banisteriopsis; Calyx glands; Histochemistry; Leaf glands; Lipids; Micromorphology; Polysaccharides; Protein.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000100112

Antiherbivory defense mechanisms along an environmental gradient in restinga
Provedor de dados: 39
Autores: Silva,Maiara Matilde da; Boeger,Maria Regina Torres; Melo Júnior,João Carlos Ferreira de; Santos,Bruno Francisco Sant’Anna dos.
ABSTRACT Plant-herbivore interactions have directed the evolution of defense mechanisms in plants, which can vary intraspecifically according to environmental quality. This study evaluated variation in the production of antiherbivory defenses in plant species distributed along an environmental gradient of restinga. The study was performed in Parque Estadual Acaraí, state of Santa Catarina, Brazil. Three species of four plant formations with different abiotic conditions were selected for study. Specific leaf area; water, nitrogen and carbon content; and presence of phenols, tannins, lignin and alkaloids, were evaluated in leaves of ten plants per species per plant formation. The results indicated that Symphyopappus casarettoi possesses Low Nutritional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coastal plain; Herbivore-plant interaction; Histochemistry; Parque Estadual Acaraí; Physical defense; Secondary metabolites.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000400583

Corona development and floral nectaries of Asclepiadeae (Asclepiadoideae, Apocynaceae)
Provedor de dados: 39
Autores: Monteiro,Mariana Maciel; Demarco,Diego.
ABSTRACT Flowers of Asclepiadoideae are notable for possessing numerous nectaries and elaborate coronas, where nectar can accumulate but is not necessarily produced. Given the complexity and importance of these structures for reproduction, this study aimed to analyze the ontogeny of the corona, the structure and position of nectaries and the histochemistry of the nectar of species of Asclepiadeae. Two types of coronas were observed: androecial [C(is)] and corolline (Ca). The development of the C(is)-type of corona initiates opposite the stamens in all species examined with the exception of Matelea in which it begins to develop as a ring around the filament tube. Despite their morphological variation, coronas typically originate from the androecium. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asclepiads; Histochemistry; Ontogeny; Nectar; Structural; Diversity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300420

Nutritious tissue in petals of Annonaceae and its function in pollination by scarab beetles
Provedor de dados: 39
Autores: Gottsberger,Gerhard; Webber,Antonio Carlos.
ABSTRACT The feeding of pollinating dynastid-scarab beetles on nutritious tissue of Annonaceae flowers results in macroscopically visible gnawing marks on petals. In the present paper, we present and discuss examples of such gnawing marks on Annonaceae from the Cerrado and the Amazon Forest in Brazil. The localization of gnawing marks on the petals and the histochemistry of the nutritious tissues are emphasized. In some species, nutritious tissue is apparently distributed among all petals, while in other species it is more or less diffusely localized. There are also cases in which nutritious tissue occurs only on clearly localized regions of the inner petals. Petals of selected Amazon species were stained, and studied by light and scanning electron...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado and Amazon forest Annonaceae; Electron microscopy; Histochemistry; Lipids; Mucilage cells in petals; Starch; Tannins.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000200279

Leaf anatomy and histochemistry of Macropeplus dentatus (Perkins) I. Santos &amp;amp; Peixoto and Macropeplus ligustrinus (Tul.) Perkins (Monimiaceae)
Provedor de dados: 39
Autores: Costa,Fernanda Flores; Lima,Helena Regina Pinto; Cunha,Maura Da; Santos,Inês da Silva.
The Monimiaceae is represented in the Neotropical region by six genera. Macropeplus Perkins is a Brazilian genus comprising four species restricted to high-montane Atlantic Forest areas, which have not been properly examined from the anatomical point of view. This study characterizes the anatomical structure and histochemical aspects of the leaves of Macropeplus dentatus and M. ligustrinus, and adds to our taxonomic knowledge ofthe genus. The individuals studied were collected in the Serra dos Órgãos National Park and in the Caraça Natural Park in southeastern Brazil. Among the characteristics common to the two species, hypostomatic leaves, dorsiventral structure, paracytic stomata, a subepidermal layer, collateral vascular system in the form of an arc in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atlantic Rain Forest; Histochemistry; Leaf anatomy; Macropeplus; Monimiaceae.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000300027

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)
Provedor de dados: 39
Autores: Soares,Taliane Leila; Jesus,Onildo Nunes de; Souza,Everton Hilo de; Santos-Serejo,Janay Almeida dos; Oliveira,Eder Jorge de.
The characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Passiflora setacea DC. Pollen morphology descriptions were made using light microscopy and scanning electron microscopy, whereas the viability analysis was performed by in vitro germination and histochemical analysis (Lugol's solution and 2,3,5-triphenyltetrazolium chloride). Pollen grains assessed for germination were inoculated in culture medium containing Ca(NO3).4H2O (0.03%), Mg(SO4).7H2O (0.02%), KNO3 (0.01%), H3BO3 (0.01%), sucrose (15%) and agar (0.8%)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro germination; Histochemistry; Scanning electron microscopy; Passifloraceae; Pollen tube.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400018

A reduced, yet functional, nectary disk integrates a complex system of floral nectar secretion in the genus Zeyheria (Bignoniaceae)
Provedor de dados: 39
Autores: Machado,Silvia Rodrigues; Souza,Camila Vaz de; Guimarães,and Elza.
ABSTRACT The genus Zeyheria (Bignoniaceae) comprises only two species, both of which have been described as possessing a reduced and non-functional nectary disk. Despite the importance of this evolutionary change in the floral nectary, these functional assumptions have been based on disk size and on the distribution, abundance and histochemistry of corolla-borne trichomes. By combining methods on light and electron microscopy, here we investigated the functionality of the reduced nectary disk and describe all of the tissues and structures of the nectar chamber in order to determine the sites of floral nectar secretion in both Zeyheria species. . Our data find the floral nectary traits of both species to be very similar, although differing in their cellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bignoniaceae; Floral nectary; Functional anatomy; Histochemistry; Ultrastructure.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300344

Histochemical changes during the ontogeny of malagueta and biquinho pepper seeds
Provedor de dados: 1
Autores: Abud, Haynna Fernandes; Araújo, Eduardo Fontes; Araujo, Roberto Fontes; Picoli, Edgard Augusto de Toledo; Gallão, Maria Izabel.
This study aimed to evaluate the structural changes and reserve deposition of malagueta and biquinho pepper seeds and to determine their chemical composition during ontogeny. The fruits were collected 25, 40, 55, 70, 85, and 100 days after anthesis (DAA). After collection, the seeds were extracted and fixed in FAA 50 and then stored in 70% ethanol. Subsequently, the seeds were embedded in methacrylate (Historesin) and sectioned with a microtome. The sections were stained with Toluidine blue O for anatomical analysis and Xylidine Ponceau, Periodic acid Schiff (PAS) and Lugol’s iodine solution for histochemical analysis. Sections were made using a cryomicrotome for acid phloroglucinol staining and Sudan III tests. Progressive lignification of the seed coat...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum frutescens; C. chinense; Chemical composition; Histochemistry; Reserves.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32492

Sublethal effect of concentrations of Metarhizium anisopliae (metsch.) sorok on the larval stage and immunologic characteristics of Diatraea flavipennella (box) (lepidoptera: crambidae)
Provedor de dados: 42
Autores: GUIMARÃES,JENNIFER; MARQUES,EDMILSON J.; WANDERLEY-TEIXEIRA,VALÉRIA; ALBUQUERQUE,AURISTELA C. DE; PASSOS,ELIANA M. DOS; SILVA,CÍNTHIA C.M.; TEIXEIRA,ÁLVARO A.C.
This work aimed to evaluate the effects of Metarhizium anisopliae on Diatraea flavipennella and investigate their immune response. Was observed the effect of M. anisopliae against larvae of D. flavipennella sprayed at concentrations of 103, 104, 105 conidia / mL, in which showed differences relative the larval period, extending up to 72.0 days in treatment and 25.0 days in the control. The results for hemocytes revealed that the most frequent cells when sprayed at the concentrations of 103, 105, 107 conidia / mL were the prohemocytes, spherulocytes, plasmatocytes and granulocytes in relation to adipohemocytes and oenocytoids. The level of nitric oxide was different between the control and the concentration 107 spores / mL (24), while the activity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogen; Hemocytes; Histochemistry; Nitric oxide; Phenoloxidases; Sugarcane borer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401973

Trichomes and chemical composition of the volatile oil of Trichogonia cinerea (Gardner) R. M. King &amp;amp; H. Rob. (Eupatorieae, Asteraceae)
Provedor de dados: 42
Autores: FERNANDES,YANNE S.; TRINDADE,LUMA M.P.; REZENDE,MARIA HELENA; PAULA,JOSÉ R.; GONÇALVES,LETÍCIA A.
Trichogonia cinerea is endemic to Brazil and occurs in areas of cerrado and campo rupestre. In this study, we characterized the glandular and non-glandular trichomes on the aerial parts of this species, determined the principal events in the development of the former, and identified the main constituents of the volatile oil produced in its aerial organs. Fully expanded leaves, internodes, florets, involucral bracts, and stem apices were used for the characterization of trichomes. Leaves, internodes, florets, and involucral bracts were examined by light microscopy and scanning electron microscopy, whereas stem apices were examined only by light microscopy. Branches in the reproductive phase were used for the extraction and determination of the composition...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Volatile oil; Histochemistry; Ontogeny; Secretory structure.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100309

Anatomical, phytochemical and histochemical study of Solidago chilensis Meyen
Provedor de dados: 42
Autores: SOUZA,DOUGLAS M.F. DE; SÁ,RAFAELA D.; ARAÚJO,EVANI L.; RANDAU,KARINA P.
ABSTRACT Solidago chilensis Meyen, belonging to the family Asteraceae, is a plant native to South America and the only representative of the genus in Brazil. This species is popularly known as “arnica” and is used to treat bruises, muscle pain and inflammation. The objective of this work was to contribute to the pharmacognostic standardization of S. chilensis. Cross-sections were obtained, by freehand, for microscopic analysis of root, stem and leaf; for these parts of the plant maceration was also performed according to the method of Jeffrey. For the leaf were still made paradermal sections, scanning electron microscopy analysis, phytochemical and histochemical tests. Thus, it was determined anatomical features useful for diagnosis of the species which,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Arnica; Asteraceae; Histochemistry; Phytochemistry; Solidago chilensis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502107

All green, but equal? Morphological traits and ecological implications on spores of three species of mosses in the Brazilian Atlantic forest
Provedor de dados: 42
Autores: MACIEL-SILVA,ADAÍSES S.; SILVA,FLÁVIA C.L. DA; VÁLIO,IVANY F.M.
Spores of the tropical mosses Pyrrhobryum spiniforme, Neckeropsis undulata and N. disticha were characterized regarding size, number per capsule and viability. Chemical substances were analyzed for P. spiniforme and N. undulata spores. Length of sporophyte seta (spore dispersal ability) was analyzed for P. spiniforme. Four to six colonies per species in each site (lowland and highland areas of an Atlantic Forest; Serra do Mar State Park, Brazil) were visited for the collection of capsules (2008 – 2009). Neckeropsis undulata in the highland area produced the largest spores (ca. 19 µm) with the highest viability. The smallest spores were found in N. disticha in the lowland (ca. 13 µm). Pyrrhobryum spiniforme produced more spores per capsule in the highland...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bryophytes; Histochemistry; Life histories; Spore size; Spore production; Tropical rain forest.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301249

Morphological and histochemical study of the masseter muscle after occlusal alteration
Provedor de dados: 47
Autores: Mardegan Issa,João Paulo; Tiossi,Rodrigo; Mizusaki Iyomasa,Mamie.
The aim of this study was to evaluate alterations in the masseter muscle of 30 male guinea-pigs submitted to occlusal alteration. The animals were divided into 2 equal groups, the control group (C) only submitted to surgical stress, and the occlusal altered group (T) submitted to teeth extraction. Each group was subdivided into 3 groups, with 5 animals, for the following studies: macroscopy and vessels distribution, light microscopy and histochemical analysis, with animals' perfusion 2 months after surgery. Data were submitted to statistical analysis. Macroscopically, an anteroposterior orientation of the muscular fibers was found and statistical difference between C and T groups in width (p&lt;0.05). Microscopically, C and T groups showed polygonal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Masseter muscle; Morphology; Histology; Histochemistry.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300003

Structural, hystochemical and cytochemical characteristics of the stigma and style in Passiflora edulis f. flavicarpa (Passifloraceae)
Provedor de dados: 52
Autores: Souza,Margarete Magalhães; Pereira,Telma Nair Santana; Dias,Angelo José Burla; Ribeiro,Beatriz Ferreira; Viana,Alexandre Pio.
Studies were carried out on anatomic aspects of the stigma and style of yellow passionfruit. The stigmatic style consisted of papillae, many layers of sub-epidermal cells and a central transmitting tissue. The stigma was of dry, unbranched, multicellular multiseriate papillate type and the cells were highly vacuolated with walls that were relatively thin consisting of compactly arranged fibrils. The style was solid with a central core of transmitting tissue that had intercellular spaces containing a matrix that included polysaccharidic and lipidic substances. During development, cells of the transmitting tissue were arranged compactly while the mature pistil showed cells with a little more loosely arranged intercellular spaces. Both the mature stigma and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yellow passionfruit; Anatomy; Stigma and style; Histochemistry; Cytochemistry.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100011

Morphological and histochemical analysis of the antennal gland of Macrobrachium Potiuna (Muller, 1880) (Crustácea, Decapoda, Palaemonidae)
Provedor de dados: 52
Autores: Moraes,Neci; Scaff,Rejane Cirra.
The objective of our study was to describe by histologic methods the structural organization of the antennal gland of M. potiuna as well as the location through histochemical methods of the neutral and sulfated polissacharides that exist in these regions. This study showed that the gland is composed of the a non-secretive tubular portion and a secretive portion which is formed by a cubic simple epithelium, sub-epithelium layer and cellular chains
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antennal gland; Macrobrachium potiuna; Morphology; Histochemistry.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300005

Storage sites in seeds of Caesalpinia echinata and C. ferrea (Leguminosae) with considerations on nutrients flow
Provedor de dados: 52
Autores: Teixeira,Simone de Pádua; Machado,Sílvia Rodrigues.
The seeds of Caesalpinia echinata and C. ferrea behaved as typical endospermic seeds, despite their different morphological classification (exendospermic seeds were described for C. echinata and endospermic seeds for C. ferrea). Then, the aim of this work was to compare, under ultrastructural and histochemical terms, the nature of the storage substances and their accumulation sites, as well as the nutrient flow in seeds of these species. Cotyledons in C. echinata accumulate carbohydrates, lipids and proteins, which are mobilized from the outer to the inner parts as revealed by the position of plasmodesmata. Endosperm in C. ferrea accumulates carbohydrates and in C. echinata accumulates substances during the initial embryogenic phases. Such tissue develops...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotyledon; Embryo; Endosperm; Histochemistry; Reserve substances; Ultrastructure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100016

Effect of salbutamol on innervated and denervated rat soleus muscle
Provedor de dados: 56
Autores: Šoic-Vranic,T.; Bobinac,D.; Bajek,S.; Jerkovic,R.; Malnar-Dragojevic,D.; Nikolic,M.
The objective of the present investigation was to perform a 14-day time-course study of treatment with salbutamol, a ß2 adrenoceptor agonist, on rat soleus muscle in order to assess fiber type selectivity in the hypertrophic response and fiber type composition. Male Wistar rats were divided into four groups: control (N = 10), treated with salbutamol (N = 30), denervated (N = 30), and treated with salbutamol after denervation (N = 30). Salbutamol was injected intraperitoneally in the rats of the 2nd and 4th groups at a concentration of 0.3 mg/kg twice a day for 2 weeks. The muscles were denervated using the crush method with pean. The animals were sacrificed 3, 6, 9, 12, and 14 days after treatment. Frozen cross-sections of soleus muscle were stained for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salbutamol; Rat skeletal muscle; Soleus muscle; Denervation; Histochemistry; Muscle fibers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200008

Effects of sciatic nerve transection on ultrastructure, NADPH-diaphorase reaction and serotonin-, tyrosine hydroxylase-, c-Fos-, glucose transporter 1- and 3-like immunoreactivities in frog dorsal root ganglion
Provedor de dados: 56
Autores: Rigon,F.; Rossato,D.; Auler,V.B.; Dal Bosco,L.; Faccioni-Heuser,M.C.; Partata,W.A.
Frogs have been used as an alternative model to study pain mechanisms. Since we did not find any reports on the effects of sciatic nerve transection (SNT) on the ultrastructure and pattern of metabolic substances in frog dorsal root ganglion (DRG) cells, in the present study, 18 adult male frogs (Rana catesbeiana) were divided into three experimental groups: naive (frogs not subjected to surgical manipulation), sham (frogs in which all surgical procedures to expose the sciatic nerve were used except transection of the nerve), and SNT (frogs in which the sciatic nerve was exposed and transected). After 3 days, the bilateral DRG of the sciatic nerve was collected and used for transmission electron microscopy. Immunohistochemistry was used to detect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Axotomy; Immunoreactivity; Histochemistry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600513

Morphometry and acetylcholinesterase activity of the myenteric plexus of the wild mouse Calomys callosus
Provedor de dados: 56
The myenteric plexus of the digestive tract of the wild mouse Calomys callosus was examined using a histochemical method that selectively stains nerve cells, and the acetylcholinesterase (AChE) histochemical technique in whole-mount preparations. Neuronal density was 1,500 ± 116 neurons/cm2 (mean ± SEM) in the esophagus, 8,900 ± 1,518 in the stomach, 9,000 ± 711 in the jejunum and 13,100 ± 2,089 in the colon. The difference in neuronal density between the esophagus and other regions was statistically significant. The neuron profile area ranged from 45 to 1,100 µm2. The difference in nerve cell size between the jejunum and other regions was statistically significant. AChE-positive nerve fibers were distributed within the myenteric plexus which is formed by...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myenteric plexus; Wild mouse; Calomys callosus; Morphometry; Histochemistry.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500009

Studies on the intrinsic nervous system of the wild rodent Calomys callosus digestive tract. II: The submucous plexus
Provedor de dados: 56
Autores: Souza,N.B.; Liberti,E.A.; De-Souza,R.R.
The submucous plexus of the normal small and large intestine of Calomys callosus was studied by NADH and AChE histochemical techniques and by transmission and scanning electron microscopy. The plexus contains (mean ± SD) 7,488 ± 293 neurons/cm2 in the duodenum, 5,611 ± 836 in the jejunum, 2,741 ± 360 in the ileum, 3,067 ± 179 in the cecum, and 3,817 ± 256 in the proximal colon. No ganglia or nerve cell bodies were seen in the esophagus, stomach, distal colon or rectum. The neurons are pear-shaped with a round or oval nucleus and the neuronal cell profile areas were larger in the large intestine than in the small intestine. Most of the neurons display intense AChE activity in the cytoplasm. AChE-positive nerve fibers are present in a primary meshwork of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Submucous plexus; Wild mouse; Morphometry; Histochemistry; Ultrastructure; Calomys callosus.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500007

Pharmacobotanical study of Manilkara zapota (L.) P.Royen (Sapotaceae)
Provedor de dados: 60
Autores: Moura,Bruna Izabela de Vasconcelos; Araújo,Bárbara Priscylla Lira de; Sá,Rafaela Damasceno; Randau,Karina Perrelli.
Manilkara zapota (L.) P. Royen, popularly known as sapoti or sapota (sapodilla), is a tree bearing an important fruit, in addition to different parts of the plant being widely used in folk medicine in the management of inflammation, pain, fevers, coughs, diarrhea, dysentery, among other ailments. This study aimed to conduct a pharmacobotany standardization study of M. zapota. Semi-permanent slides, containing transversal sections of stem, petiole, leaf blade and fruit; and paradermic sections of leaf blade were prepared, and analyzed by light microscopy. Histochemical tests were also performed in cross-sections of the leaf blade. Microscopic analysis allowed the identification of important elements in the diagnosis of the species; while the use of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Histochemistry; Sapota; Sapoti; Manilkara/drug effects; Sapotaceae/drug effects.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100547

Light exercise causes adaptive changes in muscles of young Brasileiro de Hipismo horses
Provedor de dados: 65
Autores: D'Angelis,Flora Helena de Freitas; Silva,Marco Augusto Giannoccaro da; Martins,Carla Braga; Ferraz,Guilherme de Camargo; Oliveira,João Ademir de; Boleli,Isabel Cristina; Lacerda-Neto,José Corrêa de; Queiroz-Neto,Antonio de.
This study aimed to determine the changes in the Gluteus medius of 4 year-old Brasileiro de Hipismo (BH) horses submitted to light physical activity for 10 months. The study involved 11 horses from the "Nove de Julho" Battalion of the Military Police of São Paulo State (PM-SP). Percutaneous muscle biopsy was performed in horses at maintenance and in those that had participated in routine police work for 10 months with the PM-SP. Fragments of the left Gluteus medius muscle was removed at depths of 20mm and 60mm. To determine the fiber types, the histochemical analyses were performed for myofibrillar adenosine triphosphatase (mATPase) and nicotinamide adenine dinucleotide tetrazolium reductase (NADH-TR). The morphometry of the fibers was performed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brasileiro de Hipismo breed; Horse; Histochemistry; Gluteus medius muscle; Low intensity exercise.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000500017