Consulta: subjectFacets:"Human"
Registros recuperados: 29
Data/hora: 10/06/2024 02:53:28

CLIMATE CHANGE AND RURAL WORKERS THERMAL COMFORT: HISTORICAL AND FUTURE IMPACTS
Provedor de dados: Engenharia Agrícola
Autores: Amaro,André L. N.; Yanagi Junior,Tadayuki; Yanagi,Sílvia de N. M.; Ferraz,Gabriel A. E S.; Campos,Alessandro T.
Abstract The aim of the present research was to propose a bioclimatic mapping to classify the thermal comfort and discomfort of rural workers within the state of Minas Gerais, considering historical and future scenarios. Monthly historical series (1976-2014) of minimum, mean and maximum temperature-humidity index (THI), determined through the values of air temperature (minimum, mean and maximum) and relative humidity from 48 weather stations located in the state of Minas Gerais were used to analyze the trends through Mann-Kendall and linear regression assays. The bioclimatic mapping of human comfort, obtained via geostatistical analysis, was developed as a function of the minimum, medium and maximum THI for the historical period (1976-2014) and future...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human; Biometeorology; Human thermal comfort; THI; Time series.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162018000200173

True polyploid meiosis in the human male
Provedor de dados: Genetics and Molecular Biology
Autores: Pearson,Peter L.; Madan,Kamlesh.
Abstract Polyploidy does not usually occur in germinal cells of mammals and other higher vertebrates. We describe a unique example of mosaic autotetraploidy in the meiosis of a human male. Although the original observations were made in the late 1960s, we did not publish them at that time, because we expected to detect further examples that could be described together. However, this did not occur and we have now decided to make the observations available to demonstrate that polyploidy in mammalian male meiosis can arise at a higher frequency than expected by random polyploidization of individual meiotic cells, by either DNA duplication or cell fusion prior to synapsis. This is the first description of a population of primary spermatocytes exhibiting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyploidy; Meiosis; Human; Male.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300410

Comparative assessment of human and farm animal faecal microbiota using real-time quantitative PCR
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Furet, Jean-pierre; Firmesse, Olivier; Gourmelon, Michele; Bridonneau, Chantal; Tap, Julien; Mondot, Stanislas; Dore, Joel; Corthier, Gerard.
Pollution of the environment by human and animal faecal pollution affects the safety of shellfish, drinking water and recreational beaches. To pinpoint the origin of contaminations, it is essential to define the differences between human microbiota and that of farm animals. A strategy based on real-time quantitative PCR (qPCR) assays was therefore developed and applied to compare the composition of intestinal microbiota of these two groups. Primers were designed to quantify the 16S rRNA gene from dominant and subdominant bacterial groups. TaqMan((R)) probes were defined for the qPCR technique used for dominant microbiota. Human faecal microbiota was compared with that of farm animals using faecal samples collected from rabbits, goats, horses, pigs, sheep...
Tipo: Text
Palavras-chave: Quantitative PCR; Faecal microbiota; Human; Farm animals.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00000/11071/11297.pdf

Molecular cloning of flounder Xp18, a newly identified highly conserved protein mainly expressed in the ovary
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cachot, Jerome; Bultelle, Florence; Drouot, Laurent; Galgani, Francois; Frebourg, Thierry; Leboulenger, François; Danger, Jean-michel.
Screening of a flounder ovary cDNA library with a rainbow trout p53 probe led to the isolation of a p53-unrelated cDNA encoding an unknown 161 amino acid protein. In view of its apparent molecular weight and yet unknown function, the deduced protein was named Xp18. Corresponding orthologous cDNAs or expressed sequence tags have been identified in several species including human, rodents, bovine, chicken and zebrafish and a related cDNA has also been isolated in the fruit fly. Deduced amino acid sequences appeared to be extremely well conserved throughout vertebrate evolution. Structure predictions suggested that Xp 18 may correspond to an integral protein comprising four transmembrane domains. The charged C-termini of all known vertebrate Xp18-like...
Tipo: Text
Palavras-chave: X chromosome; Brain; Ovary; Human; Teleostean; Platichthys flesus.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-756.pdf

Diagnostic moléculaire de l'origine des contaminations fécales dans l'environnement littoral - Développement de marqueurs Bacteroidales spécifiques de l'hôte
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mieszkin, Sophie.
Human and animal faecal pollution affects environmental water in inland and coastal areas, with negative implications for recreational uses, public safety and shellfish sanitary status due to the presence of enteric pathogens. Starting from 2011, the revised Bathing Water European Directive (2006/7/CE) requires the establishment of bathing water profiles with an inventory and study of the pollution sources likely to affect water quality. The faecal microbiological indicators used in these regulations, Escherichia coli and enteroccoci, cannot distinguish between human and animal faecal contamination. Thus, alternative methods, that focus on target microorganisms such as bacteria belonging to the Bacteroidales order were developed or are being developed to...
Tipo: Text
Palavras-chave: Contaminations fécales humaines; Porcines et ruminants; Bacteroidales; Gènes codant les ARNr 16S; PCR quantitative en temps réel; Eaux; Persistance; Huîtres; Human; Porcine and ruminant faecal contaminations; Bacteroidales; 16S rRNA genes; Quantitative real-time PCR; Water; Persistence; Oyster.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00021/13206/10258.pdf

Comparative Scanning Electron Microscopy of Platelets and Fibrin Networks of Human and Differents Animals
Provedor de dados: International Journal of Morphology
Autores: Pretorius,E; Vieira,W. A; Oberholzer,H. M; Auer,R. E. J.
The purpose of the present study was to compare the platelet and fibrin network ultrastructure of humans to eight different animal species in order to determine the differences between human and animal platelet and fibrin morphology, and to determine whether the animals studied differ in their platelet and fibrin morphology, and whether these differences can be observed by scanning electron microscopy. Platelets and fibrin networks play an important role both in the coagulation process as well as physiologically in allergic processes and immunological mechanisms. The thickness of human fibrin networks were compared to mouse (Mus musculus), equine (Equus caballus), vervet monkey (Chlorocebus aethiops previously Cercopithecus aethiops), oryx (Oryx gazella),...
Tipo: Journal article
Palavras-chave: Scanning electron microscopy; Platelets; Fibrin networks; Human; Animals.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000100013

Analysis of Bone Variations of the Occipital Bone in Man
Provedor de dados: International Journal of Morphology
Autores: da Mata,João Roberto; da Mata,Fabiana Ribe; Aversi-Ferreira,Tales Alexandre.
Inadequate ossification of the interparietal region induces the appearance of interparietal and wormian bones, also associated with genetic factors. The formation of the interparietal bone depends on the separation of the intermediate segment from the lateral plate by the transverse occipital suture, which means that this bone is formed by the medial and lateral plates. Wormian interparietal bones or epactal bones are located within the interparietal region, being single or multiple, and are located in the upper central region of the interparietal region, the sutural bones, however, are formed from additional ossification centers that can occur in near sutures. The aim of this work was to macroscopically evaluated the presence of changes in the structure...
Tipo: Journal article
Palavras-chave: Skull; Human; Interparietal bone; Sutural bone.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100035

Comparative Morphometry of the Olfactory Bulb, Tract and Stria in the Human, Dog and Goat
Provedor de dados: International Journal of Morphology
Autores: Kavoi,Boniface M; Jameela,Hassanali.
Morphometric parameters of olfactory brain components show species-dependent variations. However, the association of these parameters with olfactory function vis-à-vis ecological and evolutionary behaviors is poorly understood. In this study, a morphometric analysis of the olfactory bulb, tract and stria was carried out in three ecologically diverse animals comprising humans (primate), dogs (carnivore) and goats (herbivore) to elucidate differences in morphometry in relation to olfactory function. Using formalin-fixed brains, volumes and linear measurements of the olfactory structures were determined and correlated with those of cerebrum and the whole brain. The volume of the olfactory bulb was greatest in dogs, followed by goats and humans and constituted...
Tipo: Journal article
Palavras-chave: Morphometry; Olfactory brain; Human; Dog; Goat.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300047

Triple Right Renal Vein: An Uncommon Variation
Provedor de dados: International Journal of Morphology
Autores: Fernandes,R. M. P.; Conte,F. H. P.; Favorito,L. A.; Abidu-Figueiredo,M.; Babinski,M. A.
We report an unilateral three renal veins the right kidney in an elderly male cadaver. They were three calibrous veins having independent courses up to the inferior vena cava. No other vascular anomalies were noted in this specimen. This anatomical curiosity should be kept in mind by clinicians and academics that may manipulate this anatomical area
Tipo: Journal article
Palavras-chave: Anatomy; Anomalies; Human; Kidney; Vein.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300007

CHORDAE TENDINEAE ARCHITECTURE IN THE PAPILLARY MUSCLE INSERTION
Provedor de dados: International Journal of Morphology
Autores: Wang Gusukuma,Luciana; Prates,José Carlos; Smith,Ricardo Luiz.
Chordae tendineae architecture and their junction with papillary muscle were studied with scanning electron microscopy, comparing human and swine hearts. Papillary muscles with the corresponding chordae tendineae were obtained from human and pig (Sus scrofa) hearts after exposition of the left ventricle cavity, fixed with 4% formaldehyde. The specimens were sectioned in small samples and divided in four groups, a control one and the others submitted to digestion in a solution of 2.5% sodium hypochlorite in saline solution during 5, 10 and 15 minutes respectively. The specimens were washed and fixed in 1% osmium tetroxide in cacodylate buffer, kept in 1% tannic acid for 30 minutes, dehydrated and critical point dried, mounted and sputtered with gold. The...
Tipo: Journal article
Palavras-chave: Chordae tendineae; Architecture; Human; Pigs; Scanning electron microscopy.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400005

Study on the Morphology and Frequency of the Vomeronasal Organ in Humans
Provedor de dados: International Journal of Morphology
Autores: Carvalho,Maria de Fátima Pereira de; Alves,Adriana Leal; Barros,Mirna Duarte.
The vomeronasal organ was first described in humans in the seventeenth century. It has a chemosensory function and is found in the mucosa of the nasal septum of mammals and consists of an opening in the mucosa at the base of the nasal septum. For this study, 143 individulas undergoing nasofibrolaryngoscopy were studied, and presence of the vomeronasal organ was considered to be a finding from the examination. Three morphological types of vomeronasal organ were observed: fissure, fossette and circular. The total prevalence of the vomeronasal organ among these patients was 28% (40 individulas ). The prevalence of the vomeronasal organ in this study population is compatible with what has been reported in other studies. The forms of the vomeronasal organ can...
Tipo: Journal article
Palavras-chave: Vomeronasal organ; Human; Anatomy.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000200006

Bronchial tree Architecture in Mammals of Diverse Body Mass
Provedor de dados: International Journal of Morphology
Autores: Monteiro,Adilson; Smith,Ricardo Luiz.
The anatomy of mammal's lung air space constitutes the bronchial tree which disposition is associated to air flux dynamics. Casts obtained from human, pig and rat lungs were studied to analyze possible differences of the bronchial tree architecture in mammals with diverse dimensions and posture. Air spaces were filled with polymers through trachea followed by acid corrosion. Tracheal and main bronchial division's diameters were measured to relate with body mass using allometry. The results revealed a dichotomic bronchial branching pattern in the human casts and a monopodial pattern in animals. In allometric relationship trachea was larger in rats, then pigs and lastly in humans, differences were statistically significant, the same occurs in right bronchus,...
Tipo: Journal article
Palavras-chave: Bronchia; Anatomy; Human; Pig; Rat.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100050

Regression and Simulation Models for Human and Baboon Brain Parameters
Provedor de dados: International Journal of Morphology
Autores: Pokhariyal,Ganesh; Hassanali,Jameela.
The variations in morphometric parameter of mammalian brains may be influenced by process of functional complexity, evolution and adaptation. Comparative analysis of linear measurements of cerebrum in the human and baboon has shown morphometric differences. In the present study linear measurements from human and baboon cerebrum (n=10 each) were used to predict various values for human and baboon brain and body parameters through multiple regression models. The average brain weights were found to be 2.08% and 0.84% of the body weights for humans and baboons respectively. The elasticity of regression models revealed that unit percentage increase in Occipital-Frontal (OF) distance would increase the human brain weight by 66.19%, while the baboon brain weight...
Tipo: Journal article
Palavras-chave: Morphometric parameters; Human; Baboon; Brains; Regression and simulation models.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300052

Morphometry Research of Deer, Sheep, and Human Lumbar Spine: Feasibility of Using Deerand Sheep in Spinal Animal Models
Provedor de dados: International Journal of Morphology
Autores: Bai,Xiaofan; Liu,Guomin; Xu,Chuanjie; Zhuang,Yingying; Zhang,Jinlong; Jia,Yuyan; Liu,Yi.
Deer and sheep are used as spinal animal models in clinical and basic research. In this paper, the anatomical morphology, curvature, and morphology index parameters were investigated to assess the feasibility of using deer and sheep as animal models of the human spine. Fresh adult male sheep, deer, and human spine specimens (n = 10 each) were screened and subjected to morphological analyses. The statistical software package SPSS (version 17.0) was used to analyze the statistical similarity and variability among the 3 species. Deer displayed good similarity to human in terms of the vertebral transverse diameter, radius vector, spinal canal transverse diameter, radius vector, and vertebral upper and lower endplate curvature radii. Sheep displayed good...
Tipo: Journal article
Palavras-chave: Lumbar spine; Animal model; Human; Deer; Sheep; Morphometry.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200025

Anatomical Characteristics of Deer and Sheep Lumbar Spines: Comparison to the Human Lumbar Spine
Provedor de dados: International Journal of Morphology
Autores: Wang,Yang; Liu,Ting; Song,Liang-Song; Zhang,Zhi-Xin; Li,You-Qiong; Lu,Lai-Jin.
Deer and sheep spines are often used as models of the human spine. A prerequisite for the use of animal models is information regarding the interspecies differences in the parameters of general interest. This would clarify the limitations of each animal model and substantiate the applicability of the obtained results to humans. Since sufficient data appear to be currently unavailable, we sought to investigate the feasibility of using deer and sheep as animal models for studies on the human spine. The objective of this study was a thorough comparison of the anatomical parameters of deer and sheep spines with those of the human spine. We employed three-dimensional reconstructions of computed tomography images, generated using figure analysis software, which...
Tipo: Journal article
Palavras-chave: Spine; Animal model; Comparative anatomy; Sheep; Deer; Human.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100018

H9N2 avian influenza virus antibody titers in human population in fars province, Iran
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Hadipour,MM.
Among the avian influenza A virus subtypes, H5N1 and H9N2 viruses have the potential to cause an influenza pandemic because they are widely prevalent in avian species in Asia and have demonstrated the ability to infect humans. This study was carried out to determined the seroprevalence of H9N2 avian influenza virus in different human populations in Fars province, which is situated in the south of Iran. Antibodies against H9N2 avian influenza virus were measured using hemagglutination-inhibition (HI) test in sera from 300 individuals in five different population in Fars province, including poultry-farm workers, slaughter-house workers, veterinarians, patients with clinical signs of respiratory disease, and clinically normal individuals, who were not or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian influenza virus; Fars province; Human; H9N2; Iran; Seroprevalence.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000300004

Assessment of health risks and local intervention associated to living together with dogs and cats, developed at Jardim Parana, district of Brasilandia. São Paulo, Sp,- Brazil, 2003
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Gomes, Luciana Hardt; de Almeida, Marilene Fernandes; Paranhos, Noêmia Tucunduva; Garcia, Rita de Cássia; Nunes, Vânia de Fátima Plaza; Montenegro Neto, Hildebrando; Cardoso, Sônia Maria Sodré.
Objective: To carry out a situational diagnosis of interactions among man, animal and environment, to test intervention and control model in dogs and cats and lo make local population aware in order to improve human and animal health. Material and Method: The project "Evaluation of risks to the heath and local intervention associated to living together with dogs and cats" was carried out in the area invaded in Jardim Paraná, District of Brasilândia, São PauLo city, Brazil, in 2003 by the Center of Zoonosis Control of the São Paulo Municipality. Animal census, vaccinations, treatment of ectoparasitosis and endoparasitosis, animal register, animal sterilization, serological and soil examinations and removal of animals was carried through. Forty seven percent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zoonoses; Community health; Local planning; Animal health; Environment; Risk factors; Human; Animal; Interactions; Intervention; Population control; Sterilization; Dogs; Cats Zoonosis; Planificación en salud comunitaria; Estrategias locales; Salud animal; Medio ambiente y salud publica; Factores de riesgo; Hombres; Animales; Vínculo ser humano-animal; Estudios de intervención; Regulación de la población zoonoses; Planejamento em saúde comunitária; Estratégias locais; Saúde animal; Meio ambiente e saúde pública; Fatores de risco; Homens; Animais; Vínculo homem-animal; Estudos de intervenção; Controle da população; Esterilização reprodutiva; Cães; Gatos.
Ano: 2003
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3262

Prolonged exposure of human embryonic stem cells to heat shock induces necrotic cell death
Provedor de dados: Biocell
Autores: Heng,Boon Chin; Vinoth,Kumar Jayaseelan; Lu,Kai; Deng,Xuliang; Ge,Zigang; Bay,Boon Huat; Cao,Tong.
We investigated the effects of prolonged heat shock treatment on human embryonic stem cell (hESC) viability. The hESC viability steadily declined with longer exposure to heat shock treatment (43ºC). After 4 h of exposure to heat shock at 43ºC, only 56.2 ± 1.5% of cells were viable. Viability subsequently declined to 37.0 ± 3.3% and 3.5 ± 0.7% after 8 h and 16 h, respectively of heat shock treatment at 43ºC. Transmission electron micrographs showed that the morphology of the dead/dying cells after heat shock treatment was characteristic of cellular necrosis with an uncondensed chromatin and a non-intact plasma membrane. This was further confirmed by flow cytometry analysis which showed that the DNA of the dead/ dying cells was still mostly intact, unlike...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryonic; Heat shock; Human; Necrosis; Stem cells.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300007

Effect of exogenous nitric oxide on sperm motility in vitro
Provedor de dados: Biological Research
Autores: Wang,Jiangtao; He,Qingliu; Yan,Xingyu; Cai,Youmei; Chen,Junyi.
BACKGROUND: Nitric oxide (NO) has been shown to be important in sperm function, and the concentration of NO appears to determine these effects. Studies have demonstrated both positive and negative effects of NO on sperm function, but have not been able to provide a clear link between NO concentration and the extent of exposure to NO. To study the relationship between nitric oxide and sperm capacitationin vitro, and to provide a theoretical basis for the use of NO-related preparations in improving sperm motility for in vitro fertilization, we investigated the effects of NO concentration and time duration at these concentrations on in vitro sperm capacitation in both normal and abnormal sperm groups. We manipulated NO concentrations and the time duration of...
Tipo: Journal article
Palavras-chave: Nitric oxide; Sperm capacitation; Sperm motility; Time; Human.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100044

Fatal adenoviral necrotizing bronchiolitis case in a post-cardiac surgery intensive care unit
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Castelli,Jussara Bianchi; Siciliano,Rinaldo F; Vieira,Ricardo D; Aiello,Vera D; Strabelli,Tânia MV.
We report a case of a 67 year-old-male patient admitted to the intensive care unit in the post-coronary bypass surgery period who presented cardiogenic shock, acute renal failure and three episodes of sepsis, the latter with pulmonary distress at the 30th post-operative day. The patient expired within five days in spite of treatment with vancomycin, imipenem, colistimethate and amphotericin B. At autopsy severe adenovirus pneumonia was found. Viral pulmonary infections following cardiovascular surgery are uncommon. We highlight the importance of etiological diagnosis to a correct treatment approach.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Adenovirus infections; Human; Intensive care units; Bronchiolitis; Viral; Thoracic surgery.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300018

Does the change on gastrointestinal tract microbiome affects host?
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Beirão,Elisa M.; Padovan,Ana Carolina B.; Furtado,Juvêncio J.D.; Colombo,Arnaldo L.; Medeiros,Eduardo A.S.
During the past decade, studies on the composition of human microbiota and its relation to the host became one of the most explored subjects of the medical literature. The development of high-throughput molecular technologies allowed a deeper characterization of human microbiota and a better understanding of its relationship with health and disease. Changes in human habits including wide use of antimicrobials can result in dysregulation of host–microbiome homeostasis, with multiple consequences. The purpose of this review is to highlight the most important evidence in the literature of host–microbiome interactions and illustrate how these intriguing relations may lead to new treatment and prevention strategies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastrintestinal microbiome; Human.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600660

Infectious keratitis secondary to Histoplasma capsulatum: the first case reports in humans
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Arcieri,Enyr S.; Rocha,Ademir; Mendonça,Camila N.; Andreo,Eduardo G. V.; Finotti,Ioná G.A.; Furlanetto,Rafael L.; Arcieri,Rafael S.; Rocha,Flávio J.; Rizzo,Luiz V.
The authors report an unusual case of fungal keratitis caused by Histoplasma capsulatum in a male immunocompetent patient. PCR confirmed the presence of the fungus DNA in the material studied. To our knowledge this is the first reported case in humans described all over the world.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human; Keratitis; Histoplasma; Diagnosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000600013

The use of reverse transcription-PCR for the diagnosis of X-linked chronic granulomatous disease
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Agudelo-Flórez,P.; López,J.A.; Redher,J.; Carneiro-Sampaio,M.M.S.; Costa-Carvalho,B.T.; Grumach,A.S.; Condino-Neto,A.
Chronic granulomatous disease (CGD) is an inherited disorder of the innate immune system characterized by a defective oxidative burst of phagocytes and subsequent impairment of their microbicidal activity. Mutations in one of the NADPH-oxidase components affect gene expression or function of this system, leading to the phenotype of CGD. Defects in gp91-phox lead to X-linked CGD, responsible for approximately 70% of CGD cases. Investigation of the highly heterogeneous genotype of CGD patients includes mutation analysis, Northern blot or Western blot assays according to the particular case. The aim of the present study was to use reverse transcription (RT)-PCR for the analysis of molecular defects responsible for X-linked CGD in eight Brazilian patients and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide; Phagocytes; Primary immunodeficiency; Respiratory burst; Neutrophils; Human.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500001

Up-regulated expression of CD147 gene in malignant bone tumor and the possible induction mechanism during osteoclast formation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Qiao,Suchi; Liu,Chang; Xu,Weijie; AZhaTi,WuBuLi; Li,Cheng; Wang,Zhiwei.
It is increasingly evident that the microenvironment of bone can influence cancer phenotype in many ways that favor growth in bone. CD147, a transmembrane protein of the immunoglobulin (Ig) superfamily, was identified independently in different species and has many designations across different species. However, expression levels of CD147 mRNA in bone cancer have not been described. In this study, we have used real-time fluorescence quantification (RT-PCR) to demonstrate CD147 expression in malignant bone cancer and benign bone tumor tissues. The results suggested that the expression of CD147 gene was significantly up-regulated in malignant bone cancer. Moreover, we found that over-expressed RANKL progressively enhanced osteoclast formation up to 48 h,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone cancer; RT-PCR; Western blot; RANKL; Human.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900608

Association of Bartonella spp bacteremia with Chagas cardiomyopathy, endocarditis and arrythmias in patients from South America
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Corrêa,F.G.; Pontes,C.L.S.; Verzola,R.M.M.; Mateos,J.C.P.; Velho,P.E.N.F.; Schijman,A.G.; Selistre-de-Araujo,H.S.
Infection with Bartonella spp may cause cardiac arrhythmias, myocarditis and endocarditis in humans. The aim of the present study was to evaluate a possible association between Bartonella spp bacteremia and endocarditis, arrhythmia and Chagas cardiomyopathy in patients from Brazil and Argentina. We screened for the presence of bacterial 16S rRNA in human blood by PCR using oligonucleotides to amplify a 185-bp bacterial DNA fragment. Blood samples were taken from four groups of subjects in Brazil and Argentina: i) control patients without clinical disease, ii) patients with negative blood-culture endocarditis, iii) patients with arrhythmias, and iv) patients with chronic Chagas cardiomyopathy. PCR products were analyzed on 1.5% agarose gel to visualize the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human; PCR; Bartonella spp; Chagas disease; Endocarditis; Arrhythmia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700012

Evaluation of behavioral states among morning and evening active healthy individuals
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Hidalgo,M.P.; Camozzato,A.; Cardoso,L.; Preussler,C.; Nunes,C.E.; Tavares,R.; Posser,M.S.; Chaves,M.L.F.
The Horne-Östberg questionnaire partly covers some factors that may be important determinants of peak time and characterize patterns of behavior. We conducted a study for the evaluation of self-reported behavioral states (hunger sensation, availability for study, physical exercise, solving daily problems, and time preferences) as expressions of underlying cyclic activity. Three hundred and eighteen community subjects without history of medical, psychiatric, or sleep disorders were evaluated in a cross-sectional design. A self-report about daily highest level of activity was used to categorize individuals into morning, evening, and indifferently active. Time-related behavioral states were evaluated with 23 visual analog questions. The responses to most...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chronobiology; Morningness; Eveningness; Circadian rhythm; Behavior; Human.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700012

Anti-Leptospira spp and anti-Brucella spp antibodies in humans from rural area of Monte Negro municipality, state of Rondônia, Brazilian Western Amazon
Provedor de dados: Brazilian Journal of Microbiology
Autores: Aguiar,Daniel M.; Cavalcante,Guacyara T.; Camargo,Luis M. A.; Labruna,Marcelo B.; Vasconcellos,Silvio A.; Souza,Gisele O.; Gennari,Solange M.
Sera from 276 humans living in 71 farms located in Monte Negro Municipality, RO., Western Amazon, Brazil were examined for anti-Leptospira spp antibodies by Microscopic Agglutination Test and for anti-Brucella spp antibodies by Tube Agglutination Test. Leptospira spp antibodies were detected in 28 (10.2%) of them with at least one positive case in 23 farms (32.4%). The most frequent leptospira serovars were Patoc (46.7%), Autumnalis (10.0%) and Shermani (10.0%). The proportion of positive males (14.5%) were higher than females (5.0%; P &lt;0.05) and the contact with river water presented association with Leptospira spp infection (OR: 27; P=0.01). A total of 04 (1.4%) humans reacted against Brucella antigens; three farms (4.2%) presented at least one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human; Leptospirosis; Brucellosis; Amazon; Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100019

Zoonoses in humans from small rural properties in Jataizinho, Parana, Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Gonçalves,Daniela Dib; Benitez,Aline; Lopes-Mori,Fabiana Maria Ruiz; Alves,Lucimara Aparecida; Freire,Roberta Lemos; Navarro,Italmar Teodorico; Santana,Maria Aparecida Zanella; Santos,Luís Roberto Alves dos; Carreira,Teresa; Vieira,Maria Luísa; Freitas,Julio Cesar de.
The aim of this study was to conduct a serological survey for Lyme diseases, brucellosis, leptospirosis and toxoplasmosis and identify the risk variables related to these zoonoses in humans living in the rural area of Jataizinho, state of Parana, Brazil. A total of 63 rural properties were surveyed. Additionally, 207 serum samples collected from these rural area inhabitants were tested for indirect immunofluorescence (IFI) and western blots (WB) were performed to detect Borrelia burgdorferi (sensu lato); a tamponated acidified antigen test (AAT) and 2-mercaptoethanol (2-ME) were used to detect antibodies of Brucella abortus; the microscopic agglutination test (MAT) was carried out to detect antibodies anti-Leptospira spp. and IFI was used to find...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lyme disease; Leptospirosis; Brucellosis; Toxoplasmosis; Human; Antibody.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100018

Borrelia burgdorferi sensu lato in humans in a rural area of Paraná State, Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Gonçalves,Daniela Dib; Moura,Rodrigo Assunção; Nunes,Mônica; Carreira,Teresa; Vidotto,Odilon; Freitas,Julio Cesar; Vieira,Maria Luísa.
This study describes the detection of Borrelia garinii and Borrelia burgdorferi sensu stricto (s.s.) in Brazilian individuals using PCR and DNA sequencing. Our results suggest that these species are emerging pathogens in this country, and additional studies are necessary to determine the epidemiological characteristics of this disease in Brazil.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Brazil; Human; Lyme borreliosis; PCR; Sequencing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200571