Consulta: subjectFacets:"IFN-gamma"
Registros recuperados: 6
Data/hora: 01/06/2024 18:48:25

Inhibition of caspase-8 activity reduces IFN-gamma expression by T cells from Leishmania major infection
Provedor de dados: 42
Autores: Pereira,Wânia F.; Guillermo,Landi V.C.; Ribeiro-Gomes,Flávia L.; Lopes,Marcela F.
Following infection with Leishmania major, T cell activation and apoptosis can be detected in draining lymph nodes of C57BL/6-infected mice. We investigated the mechanisms involved in apoptosis and cytokine expression following Tcellactivation. After two weeks of infection, apoptotic T cells were not detected in draining lymph nodes but activation with anti-CD3 induced apoptosis in both CD4 and CD8 T cells. Treatment with anti-FasLigand, caspase-8 or caspase- 9 inhibitors did not block activation-induced T-cell death. We also investigated whether the blockade of caspase-8 activity would affect the expression of type-1 or type-2 cytokines. At early stages of infection, both CD4 and CD8 T cells expressed IFN-gamma upon activation. Treatment with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Caspase-8; CD8 T cells; IFN-gamma; Leishmaniasis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000100008

Spontaneous neutrophil activation in HTLV-1 infected patients
Provedor de dados: 55
Autores: Guerreiro,Jaqueline B.; Porto,Maria Aurélia F.; Santos,Silvane B.; Lacerda,Lino; Ho,John L.; Carvalho,Edgar M.
Human T cell lymphotropic Virus type-1 (HTLV-1) induces lymphocyte activation and proliferation, but little is known about the innate immune response due to HTLV-1 infection. We evaluated the percentage of neutrophils that metabolize Nitroblue tetrazolium (NBT) to formazan in HTLV-1 infected subjects and the association between neutrophil activation and IFN-gamma and TNF-alpha levels. Blood was collected from 35 HTLV-1 carriers, from 8 patients with HAM/TSP (HTLV-1- associated myelopathy); 22 healthy individuals were evaluated for spontaneous and lipopolysaccharide (LPS)-stimulated neutrophil activity (reduction of NBT to formazan). The production of IFN-gamma and TNF-alpha by unstimulated mononuclear cells was determined by ELISA. Spontaneous NBT levels,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HTLV-1; IFN-gamma; TNF-alpha; LPS; Neutrophil activation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600010

Normal expression of IFN-gammaR in four patients with uncommon mycobacterial infection phenotypes
Provedor de dados: 56
Autores: Rugeles,M.T.; Rincón,B.; Rugeles,C.; Montoya,C.J.; Hernández,M.; Estrada,C.; Olivares,M.M.; Patiño,P.J.
Several primary immunodeficiency diseases affecting the interleukin 12/interferon gamma (IFN-gamma) pathway have been identified, most of them characterized by recurrent and protracted infections produced by intracellular microorganisms, particularly by several species of mycobacteria. In the present study we analyzed the expression of IFN-gamma receptor (IFN-gammaR) and signal transducer and activator of transcription 1 (STAT-1) in 4 children with Mycobacterium tuberculosis infection of uncommon clinical presentation. These molecules were evaluated by flow cytometry and Western blotting in B cells transformed with Epstein-Barr virus and mutations were scanned by single-strand conformational polymorphisms and DNA sequencing. The expression of IFN-gammaR1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterial disease; IFN-gamma; IFN-gamma receptor; STAT-1; SSCP-PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900010

Myosin V and iNOS expression is enhanced in J774 murine macrophages treated with IFN-gamma
Provedor de dados: 56
Autores: Reis,D.S.; Souza,M.A.; Mineo,J.R.; Espindola,F.S.
Actin-based motor protein requirements and nitric oxide (NO) production are important features of macrophage activity during phagocytosis or microbicidal processes. Different classes of myosins contribute directly or indirectly to phagocytosis by providing mechanical force for phagosome closure or organelle movement. Recent data have shown the presence of myosins IC, II, V and IXb in phagosomes of bone marrow-derived murine macrophages. In our investigation we demonstrated the presence of different classes of myosins in J774 macrophages. We also analyzed the effect of gamma interferon (IFN-gamma), with or without calcium ionophore or cytochalasin B, on myosins as well as on inducible nitric oxide synthase (iNOS) expression and NO production. Myosins IC,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myosins; Macrophages; IFN-gamma; INOS.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200009

Patterns of intracellular cytokines in CD4 and CD8 T cells from patients with mycobacterial infections
Provedor de dados: 56
Autores: Antas,P.R.Z.; Sales,J.S.; Pereira,K.C.; Oliveira,E.B.; Cunha,K.S.; Sarno,E.N.; Sampaio,E.P.
Using a short-term bulk culture protocol designed for an intracellular-staining method based on a flow cytometry approach to the frequencies of cytokine-producing cells from tuberculosis and leprosy patients, we found distinct patterns of T cell subset expression. The method also reveals the profile of peak cytokine production and can provide simultaneous information about the phenotype of cytokine-producing cells, providing a reliable assay for monitoring the immunity of these patients. The immune response of Mycobacterium leprae and purified protein derivative (PPD) in vitro to a panel of mycobacteria-infected patients from an endemic area was assessed in primary mononuclear cell cultures. The kinetics and source of the cytokine pattern were measured at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Leprosy; IFN-gamma; TNF-alpha; Anti-hCD28 mAb.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800003

Gene expression in IFN-gamma-activated murine macrophages
Provedor de dados: 56
Autores: Pereira,C.A.; Modolell,M.; Frey,J.R.; Lefkovits,I.
Macrophages are critical for natural immunity and play a central role in specific acquired immunity. The IFN-gamma activation of macrophages derived from A/J or BALB/c mice yielded two different patterns of antiviral state in murine hepatitis virus 3 infection, which were related to a down-regulation of the main virus receptor. Using cDNA hybridization to evaluate mRNA accumulation in the cells, we were able to identify several genes that are differently up- or down-regulated by IFN-gamma in A/J (267 and 266 genes, respectively, up- and down-regulated) or BALB/c (297 and 58 genes, respectively, up- and down-regulated) mouse macrophages. Macrophages from mice with different genetic backgrounds behave differently at the molecular level and comparison of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene array; MRNA; Mouse hepatitis virus 3 (MHV3); IFN-gamma; BALB/c mouse; A/J mouse; Macrophages.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200005