Consulta: subjectFacets:"ITS"
Registros recuperados: 69
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ESTUDIO POBLACIONAL DEL RECURSO ANCHOVETA (ENGRAULIS RINGENS JENYNS 1842) (CLUPEIFORMES, ENGRAULIDAE), MEDIANTE ANALISIS DE ADN
Provedor de dados: 72
Autores: Ferrada,Sandra; Hernández,Klaudia; Montoya,R.; Galleguillos,Ricardo.
Se estudió la variabilidad de 2 genes nucleares, el intrón 4/5 del gen de Calmodulina (CaM) por amplificación PCR y la región ITS (Internal Transcribed Spacers) del ADNr, por PCR y digestión en 139 ejemplares de E. ringens provenientes de las costas de Iquique (20°13'S; 70°09'W) y Talcahuano (36°41'S; 73°06'W). Para CaM se identificaron 8 genotipos en las muestras de Talcahuano y 7 en Iquique, presentando 1 a 3 fragmentos amplificados de 550 a 1.100 pb. En la digestión del fragmento ITS amplificado, sólo la endonucleasa TaqªI detectó 3 diferentes genotipos, 2 presentes en Talcahuano y 3 en Iquique. Se discuten las frecuencias observadas para cada gen y área de estudio. Los resultados llevan a confirmar la existencia de un solo stock, desde un punto de...
Tipo: Journal article
Palavras-chave: Engraulis ringens; ITS; CaM; Variabilidad genética; Stock.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382002000200022

Relaciones filogenéticas de Rhodolirium montanum Phil. y especies afines, basadas en secuencias nucleotídicas de la región ITS y análisis cariotípico
Provedor de dados: 73
Autores: Muñoz,Manuel; Riegel,Ricardo; Seemann,Peter; Peñailillo,Patricio; Schiappacasse,Flavia; Núñez,José.
La taxonomía de los géneros chilenos de Amaryllidaceae es confusa, persistiendo problemas taxonómicos tanto a nivel de géneros como de especies. Actualmente existe controversia acerca de la correcta clasificación de especies hasta ahora asignadas a Rhodophiala C.Presl. A través del estudio de aspectos morfológicos, recientemente se ha sugerido la rehabilitación del género Rhodolirium Phil., donde Rhodolirium montanum Phil. es considerada como especie tipo. Esta especie ha sido denominada Rhodophiala rhodolirion (Baker) Traub en las clasificaciones tradicionales. Dado que los datos morfológicos han provisto información limitada y ambigua en la clasificación de las Amarilidáceas chilenas, este trabajo evalúa la pertinencia de formalizar la rehabilitación del...
Tipo: Journal article
Palavras-chave: Rhodophiala; Rhodolirium montanum; Amaryllidaceae; ITS; Cariotipo.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432011000100005

Evidencia de ADN ribosomal y del cloroplasto para la diversificaciOn de las PortulacAceas de AmErica occidental en la regiOn andina
Provedor de dados: 73
Autores: Hershkovitz,Mark A.
Se determinaron las secuencias del espaciador interno (ITS) del ADN ribosomal y del espaciador intergénico de ycf3-trnS del ADN del cloroplasto para 183 muestras de taxas, chilenos y no chilenos, de Portulacaceae de América occidental, y sus grupos externos. Los datos permiten refinar previas inferencias sobre la circunscripción e interrelaciones de los géneros estudiados. En particular estos datos revelan que una circunscripción anteriormente publicada de Cistanthe Spach es polifilética y también revelan la existencia de un clado norteamericano compuesto por Claytonia L., Lewisia Pursh, Lewisiopsis R. Govaerts, and Montia L. De los datos de los géneros sudamericanos surgieron dos patrones: (1) en algunos casos la divergencia interespecífica es...
Tipo: Journal article
Palavras-chave: Portulacaceae; Chile; ITS; Ycf3-trnS; Hibridización.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432006000100002

PHYLOGENETIC RELATIONSHIPS AMONG CHILEAN SPECIES OF DRIMYS (WTNTERACEAE) BASED ON ITS SEQUENCES AND INSERTION/DELETION EVENTS
Provedor de dados: 73
Autores: Ruiz,Eduardo; Toro,Oscar; Crawford,Daniel J; Stuessy,Tod F; Negritto,María A; Baeza,Carlos; Becerra,José.
Phylogenetic relationships among Chilean species of Drimys as well asD. granadensis from Central America andD. angustifolia from Brazil were reconstructed using internal transcribed spacers (ITS) of nuclear ribosomal DNA and a parsimony analysis. Our results at the family level were congruent with previous phylogenetic hypotheses. Molecular data indicate that the Juan Fernandez Island endemic D. confertifolia diverged early in the evolution of the genus, and it is not closely related to the continental species D. winteri, as might be expected on the basis of morphology and biogeography. The ITS phytogeny also provides general support for a recent distribution of species on the western side of the Andes Mountains; the only exception is D. andina, which may...
Tipo: Journal article
Palavras-chave: Drimys; Evolution; ITS; Indels; Winteraceae.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432008000200008

Multiple introductions and gene flow in subtropical South American populations of the fireweed, Senecio madagascariensis(Asteraceae)
Provedor de dados: 74
Autores: Mäder,Geraldo; Castro,Luana; Bonatto,Sandro Luis; Freitas,Loreta Brandão de.
Abstract Non-indigenous plants exhibit different attributes that make them aggressive competitors with indigenous plants and serious threats to biodiversity.Senecio madagascariensis (fireweed, Asteraceae), a native from southern Africa, is a strong competitor in agricultural activities and has toxic alkaloids that may result in high cattle mortality. In Brazil, this weed was collected for the first time in 1995 and has since spread quickly throughout the Pampas region. To better understand the invasion of the fireweed in South America, we used a genetic characterization with internal transcribed spacer (ITS) and microsatellite markers. Based on the ITS data, the southern Brazil populations of S. madagascariensis shared genetic homology with samples taken...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; ITS; Pampas; Weed; Spread.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100135

Molecular evidence of polyphyletism in the plant genus Carum L. (Apiaceae)
Provedor de dados: 74
Autores: Papini,Alessio; Banci,Francesca; Nardi,Enio.
An analysis of internal transcribed spacer (ITS) DNA sequences of the four species of Carum L. (Apiaceae) known in Italy revealed that this genus is polyphyletic. Maximum parsimony with bootstrap resampling, maximum likelihood and Bayesian inference analyses resulted in three distinct clades: Carum carvi L. clustered within tribe Careae Baill. (former Aegopodium clade); Hellenocarum multiflorum (= Carum multiflorum), Carum heldreichii and Carum appuanum clustered within the tribe Pyramidoptereae Boiss.; and H. multiflorum and C. heldreichii formed a well supported clade. Since the sister group of H. multiflorum and C. heldreichii was Bunium elegans the autonomy of Hellenocarum from Carum is confirmed by our study. We also found that C. appuanum clustered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apiaceae; Carum; Hellenocarum; ITS; Phylogeny.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300029

Evolutionary history of the Tip100 transposon in the genus Ipomoea
Provedor de dados: 74
Autores: Christoff,Ana-Paula; Loreto,Elgion L.S.; Sepel,Lenira M.N.
Tip100 is an Ac-like transposable element that belongs to the hAT superfamily. First discovered in Ipomoea purpurea (common morning glory), it was classified as an autonomous element capable of movement within the genome. As Tip100 data were already available in databases, the sequences of related elements in ten additional species of Ipomoea and five commercial varieties were isolated and analyzed. Evolutionary analysis based on sequence diversity in nuclear ribosomal Internal Transcribed Spacers (ITS), was also applied to compare the evolution of these elements with that of Tip100 in the Ipomoea genus. Tip100 sequences were found in I. purpurea, I. nil, I. indica and I. alba, all of which showed high levels of similarity. The results of phylogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HAT; Transposable elements; Ac-Ds; Ipomoea; Genome evolution; ITS.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300013

The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
Provedor de dados: 74
Autores: Mäder,Geraldo; Zamberlan,Priscilla M.; Fagundes,Nelson J.R.; Magnus,Tielli; Salzano,Francisco M.; Bonatto,Sandro L.; Freitas,Loreta B.
The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Intraspecific variability; ITS; Passiflora; Phylogeography.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018

Characterization of the protozoan parasite Marteilia refringens infecting the dwarf oyster Ostrea stentina in Tunisia
Provedor de dados: 5
Autores: Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle.
Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010...
Tipo: Text
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf

The name game: morpho-molecular species boundaries in the genus Psammocora (Cnidaria, Scleractinia)
Provedor de dados: 5
Autores: Benzoni, Francesca; Stefani, Fabrizio; Pichon, Michel; Galli, Paolo.
The morphometric and molecular boundaries between twelve Psammocora (Cnidaria, Scleractinia) nominal species were addressed. The type specimens of Psammocora haimiana Milne Edwards &amp; Haime, 1851, P. togianensis Umbgrove, 1940, P. folium Umbgrove, 1939, P. digitata Milne Edwards &amp; Haime, 1851, Maeandroseris australiae Rousseau, 1854, P. samoensis Hoffmeister, 1925, P. superficialis Gardiner, 1898, P. profundacella Gardiner, 1898, P. nierstraszi Van der Horst, 1921, P. verrilli Vaughan, 1907, and P. albopicta Benzoni, 2006, were analysed together with specimens from museum collections, including those depicted in widely cited taxonomic descriptions, and material collected for this study in different parts of the Indo-Pacific. Morphometric analyses of...
Tipo: Text
Palavras-chave: 5; 8S; Calice morphometry; Enclosed petaloid septa; ITS; Phylogeny; Psammocora haimiana; Psammocora vaughani; Scleractinian coral.
Ano: 2010
URL: https://archimer.ifremer.fr/doc/00138/24941/23034.pdf

Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the     Chaetoceros neogracilis     species complex
Provedor de dados: 5
Autores: Balzano, Sergio; Percopo, Isabella; Siano, Raffaele; Gourvil, Priscillia; Chanoine, Melanie; Marie, Dominique; Vaulot, Daniel; Sarno, Diana.
Seventy-five diatoms strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy (SEM and TEM) as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the genera Arcocellulus, Attheya, Chaetoceros, Cylindrotheca, Eucampia, Nitzschia, Porosira, Pseudo-nitzschia, Shionodiscus, Thalassiosira, Synedropsis. Most of the species have a distribution confined to the northern/polar area. Chaetoceros neogracilis and Chaetoceros gelidus were the most represented taxa. Strains of C. neogracilis were morphologically similar and shared identical 18S rRNA gene sequences, but belonged to four distinct genetic clades based on 28S rRNA,...
Tipo: Text
Palavras-chave: Biogeography; ITS; ITS2 secondary structure; LSU; Morphology; Phylogeny; Polar diatoms; SSU.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00356/46718/46584.pdf

Resolving the phylogenetic and taxonomic status of dark-spored teleomorph genera in the Botryosphaeriaceae
Provedor de dados: 16
Autores: Phillips, A.J.L.; Alves, A.; Pennycook, S.R.; Johnston, P.R.; Ramaley, A.; Akulov, A.; Crous:, P.W.
Species in the Botryosphaeriaceae are common plant pathogens and saprobes found on a variety of mainly woody hosts. Teleomorphs typically have hyaline, aseptate ascospores. However, some have been reported with brown ascospores and their taxonomic status is uncertain. A multi-gene approach (SSU, ITS, LSU, EF1-α and β-tubulin) was used to resolve the correct phylogenetic position of the dark-spored ‘Botryosphaeria’ teleomorphs and related asexual species. Neodeightonia and Phaeobotryon are reinstated for species with brown ascospores that are either 1-septate (Neodeightonia) or 2-septate (Phaeobotryon). Phaeobotryosphaeria is reinstated for species with brown, aseptate ascospores that bear an apiculus at either end. The status of Sphaeropsis is clarified...
Tipo: Article / Letter to the editor
Palavras-chave: Barriopsis; Diplodia; Dothiorella; EF1-α; ITS; Lasiodiplodia; LSU; Neodeightonia; Phaeobotryon; Phaeobotryosphaeria; Phylogeny; Spencermartinsia; Sphaeropsis; SSU.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/532050

Coniochaeta (Lecythophora), Collophora gen. nov. And Phaeomoniella species associated with wood necroses of Prunus trees
Provedor de dados: 16
Autores: Damm, U.; Fourie, P.H.; Crous, P.W.
Species of the genus Coniochaeta (anamorph: Lecythophora) are known as pathogens of woody hosts, but can also cause opportunistic human infections. Several fungi with conidial stages resembling Lecythophora were isolated from necrotic wood samples of Prunus trees in South Africa. In order to reveal their phylogenetic relationships, these fungi were studied on a morphological and molecular (5.8S nrDNA, ITS-1, ITS-2, GAPDH, EF-1α, 28S nrDNA, 18S nrDNA) basis. Some of the isolates were identified as Coniochaeta (Sordariomycetes), including C. velutina and two new species, C. africana and C. prunicola. The majority of the isolates, however, formed pycnidial or pseudopycnidial synanamorphs and were not closely related to Coniochaeta. According to their 28S...
Tipo: Article / Letter to the editor
Palavras-chave: Collophora; Coniochaeta; EF-1α; GAPDH; ITS; Lecythophora; LSU; Pathogenicity; Phaeomoniella; Prunus; SSU; Systematics.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/531834

Stilbosporaceae resurrected: generic reclassification and speciation
Provedor de dados: 16
Autores: Voglmayr, H.; Jaklitsch, W.M.
Following the abolishment of dual nomenclature, Stilbospora is recognised as having priority over Prosthecium. The type species of Stilbospora, S. macrosperma, is the correct name for P. ellipsosporum, the type species of Prosthecium. The closely related genus Stegonsporium is maintained as distinct from Stilbospora based on molecular phylogeny, morphology and host range. Stilbospora longicornuta and S. orientalis are described as new species from Carpinus betulus and C. orientalis, respectively. They differ from the closely related Stilbospora macrosperma, which also occurs on Carpinus, by longer, tapering gelatinous ascospore appendages and by distinct LSU, ITS rDNA, rpb2 and tef1 sequences. The asexual morphs of Stilbospora macrosperma, S. longicornuta...
Tipo: Article / Letter to the editor
Palavras-chave: Alnecium; Calospora; Calosporella; ITS; LSU; Molecular phylogeny; Phaeodiaporthe; Rpb2; Systematics; Tef1.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/532218

The largest type study of Agaricales species to date: bringing identification and nomenclature of Phlegmacium (Cortinarius) into the DNA era
Provedor de dados: 16
Autores: Liimatainen, K.; Niskanen, T.; Dima, B.; Kytövuori, I.; Ammirati, J.F.; Frøslev, T.G.
Cortinarius is a species-rich and morphologically challenging genus with a cosmopolitan distribution. Many names have not been used consistently and in some instances the same species has been described two or more times under separate names. This study focuses on subg. Phlegmacium as traditionally deﬁned and includes species from boreal and temperate areas of the northern hemisphere. Our goals for this project were to: i) study type material to determine which species already have been described; ii) stabilize the use of Friesian and other older names by choosing a neo- or epitype; iii) describe new species that were discovered during the process of studying specimens; and iv) establish an accurate ITS barcoding database for Phlegmacium species. A total...
Tipo: Article / Letter to the editor
Palavras-chave: Basidiomycota; Diversity; DNA barcoding; ITS; Taxonomy; Typiﬁcation.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/532330

Two new species of Metrosideros (Myrtaceae) from New Caledonia: dual characterisation with morphology and nrDNA sequence variation
Provedor de dados: 16
Autores: Dawson, John W.; Whitaker, Anthony H.; Whitaker, Vivienne A.; Gardner, Richard C.; Wright, Shane D.
We have identified two new species of Metrosideros subg. Mearnsia (Myrtaceae) growing on, and apparently endemic to, the massif known as the Roches d’Ouaïème in New Caledonia. Of these two species, we include M. whitakeri in sect. Mearnsia and M. rotundifolia in sect. Calyptropelala. Both morphological assessments and phylogenetic analyses based on nrDNA sequence variation produced congruent interpretations of the novelty of the new species and of their classification.
Tipo: Article / Letter to the editor
Palavras-chave: ITS; Metrosideros; Mearnsia; Myrtaceae; Nuclear rDNA.
Ano: 2000
URL: http://www.repository.naturalis.nl/record/526039

Searching for cryptic species in Erpobdella octoculata (L.) (Hirudinea: Clitellata): discordance between the results of genetic analysis and cross-breeding experiments
Provedor de dados: 16
Autores: Koperski, P.; Milanowski, R.; Krzyk, A.
The main aim of this study was to reveal reproduction barriers and potentially cryptic radiation within the very common and morphologically variable leech species - Erpobdella octoculata (L., 1758). The differences in reproductive success of the morphological forms were compared experimentally. The data based on the results of field sampling and analysis of ITS and COI sequences is also presented. The results of the analysis of DNA sequences clearly show lack of reproduction barriers between the analysed morphological forms. Subtle differences in DNA sequences between individuals, found with the use of the arbitrary primers method, seem to be related mainly to geographical distance between sub-populations. On the basis of these results, E. octoculata...
Tipo: Article / Letter to the editor
Palavras-chave: COI; Cryptic species; Discordance; Hirudinea; ITS; Molecular taxonomy; 42.76.
Ano: 2011
URL: http://www.repository.naturalis.nl/record/369712

A re-appraisal of Harknessia (Diaporthales), and the introduction of Harknessiaceae fam. nov.
Provedor de dados: 16
Autores: Crous, P.W.; Summerell, B.A.; Shivas, R.G.; Carnegie, A.J.; Groenewald, J.Z.
Harknessiaceae is introduced as a new family in the ascomycete order Diaporthales to accommodate species of Harknessia with their Wuestneia-like teleomorphs. The family is distinguished by having pycnidial conidiomata with brown, furfuraceous margins, brown conidia with hyaline, tube-like basal appendages, longitudinal striations, and rhexolytic secession. Six species occurring on Eucalyptus are newly introduced, namely H. australiensis, H. ellipsoidea, H. pseudohawaiiensis, and H. ravenstreetina from Australia, H. kleinzeeina from South Africa, and H. viterboensis from Italy. Epitypes are designated for H. spermatoidea and H. weresubiae, both also occurring on Eucalyptus. Members of Harknessia are commonly associated with leaf spots, but also occur as...
Tipo: Article / Letter to the editor
Palavras-chave: Biodiversity; Fungal pathogens; Harknessiaceae; ITS; LSU; Phylogeny; Systematics.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/532250

Taxonomy and phylogeny of European Gymnopus subsection Levipedes (Basidiomycota, Omphalotaceae)
Provedor de dados: 16
Autores: Antonín, V.; Sedlák, P.; Tomšovský, M.
The systematic integrity of European Gymnopus subsect. Levipedes is verified based on anatomicmorphological characters with support from DNA sequences of ITS and translation elongation factor 1-alpha genes. Seven species (G. alpinus, G. aquosus, G. dryophilus – including var. lanipes, G. erythropus, G. fagiphilus, G. hybridus, and G. ocior) belonging to this subsection are included. We clarify the concepts of G. dryophilus and G. ocior, which were occasionally confused in older literature. Due to unavailability of previously selected neotype of G. dryophilus the substitute neotype specimen is selected. Gymnopus dryophilus var. lanipes is confirmed as a variety; no important differences from nominotypical variability were detected. All discriminative...
Tipo: Article / Letter to the editor
Palavras-chave: ITS; Neotype; Nomenclature; Translation elongation factor 1-alpha.
Ano: 2013
URL: http://www.repository.naturalis.nl/record/532186

Novel genera and species of coniothyrium-like fungi in Montagnulaceae (Ascomycota)
Provedor de dados: 16
Autores: Verkley, G.J.M.; Dukik, K.; Renfurm, R.; Göker, M.; Stielow, J.B.
Based on analyses of concatenated internal transcribed spacer regions of the nrDNA operon (ITS), large subunit rDNA (LSU), γ-actin and β-tubulin gene sequences the taxonomy of coniothyrium-like fungi belonging in the family Montagnulaceae, order Pleosporales, was re-assessed. Two new genera are proposed, Alloconiothyrium, to accommodate A. aptrootii sp. nov., and Dendrothyrium for D. longisporum sp. nov. and D. variisporum sp. nov. One new species is described in Paraconiothyrium, viz. Parac. archidendri sp. nov., while two species so far classified in Paraconiothyrium are transferred to Paraphaeosphaeria, viz. Paraph. minitans comb. nov. and Paraph. sporulosa comb. nov. In Paraphaeosphaeria five new species are described based on asexual morphs, viz....
Tipo: Article / Letter to the editor
Palavras-chave: Γ-actin; Β-tubulin; ITS; LSU; Microsphaeropsis; Paraconiothyrium; Taxonomy.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/532399

Why everlastings don’t last
Provedor de dados: 16
Autores: Crous, P.W.; Groenewald, J.Z.
The Cape Floral Region represents one of the world’s biodiversity hot spots, with a high level of plant, animal and insect endemism. The fungi occurring in this region, however, remain poorly studied. It is widely postulated that each plant species should harbour at least five to six unique fungal species, a number that we regard to be a huge underestimate. To test this hypothesis, we decided to study a single senescent flower of Phaenocoma prolifera (‘everlasting’; Asteraceae) collected in South Africa, and posed the question as to how many different species of fungi could be isolated and cultivated from 10 leaf bracts. Using a damp chamber technique, numerous microfungi could be induced to sporulate, enabling most of them to be successfully isolated on...
Tipo: Article / Letter to the editor
Palavras-chave: Batcheloromyces; Catenulostroma; Cladosporium; Devriesia; Exophiala; ITS; LSU; Penicillium; Penidiella; Phaenocoma prolifera; Systematics; Teratosphaeria; Toxicocladosporium; Xenophacidiella.
Ano: 2011
URL: http://www.repository.naturalis.nl/record/531877

Resolving the Diaporthe species occurring on soybean in Croatia
Provedor de dados: 16
Autores: Santos, J.M.; Vrandečić, K.; Ćosić, J.; Duvnjak, T.; Phillips , A.J.L.
Diaporthe (anamorph = Phomopsis) species are plant pathogens and endophytes on a wide range of hosts including economically important crops. At least four Diaporthe taxa occur on soybean and they are responsible for serious diseases and significant yield losses. Although several studies have extensively described the culture and morphological characters of these pathogens, their taxonomy has not been fully resolved. Diaporthe and Phomopsis isolates were obtained from soybean and other plant hosts throughout Croatia. Phylogenetic relationships were determined through analyses of partial translation elongation factor 1-alpha (EF1-α) gene and ITS nrDNA sequence data. By combining morphological and molecular data, four species could be distinguished on...
Tipo: Article / Letter to the editor
Palavras-chave: Anamorph; EF1-α; ITS; Mating-types; Phylogeny; Systematics; Taxonomy; Teleomorph.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/531776

Segregation of Leptatherum from Microstegium (Andropogoneae, Poaceae) confirmed by Internal Transcribed Spacer DNA sequences
Provedor de dados: 16
Autores: Chen, C.-H.; Veldkamp, J.F.; Kuoh, C.-S.; Tsai, C.-C.; Chiang, Y.-C.
Phylogenetic analyses of Microstegium (Andropogoneae, Poaceae) and some other Andropogoneae species were conducted inferred from the ITS/5.8S sequences. As a result of this study, Microstegium is polyphyletic. There are two rather distant monophyletic clades, one with Microstegium nudum (type of Leptatherum), M. japonicum and M. somae, the other one with M. ciliatum, M. fauriei, M. geniculatum and M. vimineum (type of Microstegium). Therefore we feel justified to reinstate the genus Leptatherum and three new combinations, Leptatherum boreale, L. nudum and L. somae, are proposed.
Tipo: Article / Letter to the editor
Palavras-chave: Andropogoneae; ITS; Leptatherum; Microstegium; Phylogeny; Poaceae; Taxonomy.
Ano: 2009
URL: http://www.repository.naturalis.nl/record/524656

A phylogenetic study of Dothiorella and Spencermartinsia species associated with woody plants in Iran, New Zealand, Portugal and Spain
Provedor de dados: 16
Autores: Abdollahzadeh, J.; Javadi, A.; Zare, R.; Phillips, A.J.L.
Dothiorella and Spencermartinsia are two botryosphaeriaceous genera with dark 2-celled conidia and found in parasitic, saprophytic or endophytic association with various woody host plants. Based on ITS and EF1-α sequence data and morphology, eight new species are described from Iran, New Zealand, Portugal and Spain. Of these, five species are placed in Dothiorella, namely D. iranica, D. parva, D. prunicola, D. sempervirentis and D. striata, and three species belong to Spencermartinsia named as S. citricola, S. mangiferae and S. plurivora. An identification key to the species of each genus is provided.
Tipo: Article / Letter to the editor
Palavras-chave: Botryosphaeriaceae; Dothiorella; ITS; Phylogeny; Spencermartinsia; Systematics; Taxonomy.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/532314

Plectosphaerella species associated with root and collar rots of horticultural crops in southern Italy
Provedor de dados: 16
Autores: Carlucci, A.; Raimondo, M.L.; Santos, J.; Phillips, A.J.L.
Plectosphaerella cucumerina, most frequently encountered in its Plectosporium state, is well known as a pathogen of several plant species causing fruit, root and collar rot, and collapse. It is considered to pose a serious threat to melon (Cucumis melo) production in Italy. In the present study, an intensive sampling of diseased cucurbits as well as tomato and bell pepper was done and the fungal pathogens present on them were isolated. Phylogenetic relationships of the isolates were determined through a study of ribosomal RNA gene sequences (ITS cluster and D1/D2 domain of the 28S rRNA gene). Combining morphological, culture and molecular data, six species were distinguished. One of these (Pa. cucumerina) is already known. Four new species are described as...
Tipo: Article / Letter to the editor
Palavras-chave: D1/D2; ITS; LSU; Phylogeny; Plectosporium; RDNA; Systematics; Taxonomy.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/531914

Fungal pathogens of Proteaceae
Provedor de dados: 16
Autores: Crous, P.W.; Summerell, B.A.; Swart, L.; Denman, S.; Taylor, J.E.; Bezuidenhout, C.M.; Palm, M.E.; Marincowitz, S.; Groenewald, J.Z.
Species of Leucadendron, Leucospermum and Protea (Proteaceae) are in high demand for the international floriculture market due to their brightly coloured and textured flowers or bracts. Fungal pathogens, however, create a serious problem in cultivating flawless blooms. The aim of the present study was to characterise several of these pathogens using morphology, culture characteristics, and DNA sequence data of the rRNA-ITS and LSU genes. In some cases additional genes such as TEF 1-α and CHS were also sequenced. Based on the results of this study, several novel species and genera are described. Brunneosphaerella leaf blight is shown to be caused by three species, namely B. jonkershoekensis on Protea repens, B. nitidae sp. nov. on Protea nitida and B....
Tipo: Article / Letter to the editor
Palavras-chave: Biodiversity; Cut-flower industry; Fungal pathogens; ITS; LSU; Phylogeny; Systematics.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/532487

Trichoderma (Hypocrea) species with green ascospores from China
Provedor de dados: 16
Autores: Zhu, Z.X.; Zhuang, W.Y.
Stromata of Trichoderma species having green ascospores were collected in various regions of China. Based on morphology of the sexual and asexual morph, culture characteristics, and sequence analyses of rpb2 and tef1 genes, 17 species with green ascospores were identified. Among them, Trichoderma rosulatum, T. rufobrunneum and T. stipitatum are described as new species, and seven other species are reported for the first time from China. Trichoderma rosulatum produces small bright yellow or pale greenish stromata with dense dark green ostioles and gliocladium-like conidiophores, shows a close relationship to T. thelephoricola, and belongs to the Chlorospora clade. Trichoderma rufobrunneum, which typically forms reddish brown stromata, is recognised as a...
Tipo: Article / Letter to the editor
Palavras-chave: Ascomycota; Hypocreales; ITS; Morphology; Rpb2; Sequence analysis; Systematics; Tef1.
Ano: 2015
URL: http://www.repository.naturalis.nl/record/560056

Phylogeny and morphology of four new species of Lasiodiplodia from Iran
Provedor de dados: 16
Autores: Abdollahzadeh, J.; Javadi, A.; Mohammadi Goltapeh, E.; Zare, R.; Phillips, A.J.L.
Four new species of Lasiodiplodia; L. citricola, L. gilanensis, L. hormozganensis and L. iraniensis from various tree species in Iran are described and illustrated. The ITS and partial translation elongation factor-1α sequence data were analysed to investigate their phylogenetic relationships with other closely related species and genera. The four new species formed well-supported clades within Lasiodiplodia and were morphologically distinct from all other known species.
Tipo: Article / Letter to the editor
Palavras-chave: Botryosphaeriaceae; EF-1α; ITS; Lasiodiplodia; Phylogeny; Taxonomy.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/532326

A monograph of Otidea (Pyronemataceae, Pezizomycetes)
Provedor de dados: 16
Autores: Olariaga, I.; Vooren, N. van; Carbone, M.; Hansen, K.
The easily recognised genus Otidea is subjected to numerous problems in species identiﬁcation. A number of old names have undergone various interpretations, materials from different continents have not been compared and misidentiﬁcations occur commonly. In this context, Otidea is monographed, based on our multiple gene phylogenies assessing species boundaries and comparative morphological characters (see Hansen &amp; Olariaga 2015). All names combined in or synonymised with Otidea are dealt with. Thirty-three species are treated, with full descriptions and colour illustrations provided for 25 of these. Five new species are described, viz. O. borealis, O. brunneo­parva, O. oregonensis, O. pseudoleporina and O. subformicarum. Otidea cantharella var. minor...
Tipo: Article / Letter to the editor
Palavras-chave: Flavoscypha; ITS; ITS1 minisatellites; LSU; Otideopsis; Resinous exudates.
Ano: 2015
URL: http://www.repository.naturalis.nl/record/588730

DNA barcoding in Mucorales: an inventory of biodiversity
Provedor de dados: 16
Autores: Walther, G.; Pawłowska, J.; Alastruey-Izquierdo, A.; Wrzosek, M.; Rodriguez-Tudela, J.L.; Dolatabadi, S.; Chakrabarti, A.; Hoog, G.S. de.
The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and...
Tipo: Article / Letter to the editor
Palavras-chave: Backusella; Biodiversity; Clinical relevance; DNA barcoding; Intraspecific variability; ITS; LSU; Mucor; Mucorales; Nomenclature; Rhizomucor; Taxonomy; Zygorhynchus.
Ano: 2013
URL: http://www.repository.naturalis.nl/record/532353

Marasmioid and gymnopoid fungi of the Republic of Korea. 2. Marasmius sect. Globulares
Provedor de dados: 16
Autores: Antonín, V.; Ryoo, R.; Shin, H.-D.
Seven species of Marasmius sect. Globulares with smooth pileipellis cells (sect. Globulares s. Singer) have been collected in the Republic of Korea (South Korea) to date, viz. M. aurantioferrugineus, M. brunneospermus, M. maximus, M. nivicola, M. purpureostriatus, M. wynneae and M. fusicystidiosus. Descriptions of their macro- and microscopic features with a discussion of similar taxa are given. Their taxonomic position was confirmed using DNA data. Marasmius fusicystidiosus is described as a new species. A key to aid in their identification is also provided.
Tipo: Article / Letter to the editor
Palavras-chave: ITS; LSU; Nomenclature; Taxonomy.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/531895

Debating phylogenetic relationships of the scleractinian Psammocora: molecular and morphological evidences
Provedor de dados: 16
Autores: Benzoni, F.; Stefani, F.; Stolarski, J.; Pichon, M.; Mitta, G.; Galli, P.
The phylogenetic relationships of the scleractinian genus Psammocora with the other genera traditionally included in the family Siderastreidae and some Fungiidae are assessed based on combined skeletal and molecular data. P. explanulata differs from the other examined congeneric species (P. contigua, P. digitata, P. nierstraszi, P. profundacella, P. superficialis, and P. stellata) in possessing interstomatous septa between adult corallites, costae, and in having continuous buttress-like structures joining septal faces (i.e., fulturae) which typically occur in fungiids. These characters are shared with Coscinaraea wellsi but not with the remainder of the examined siderastreids (the congeneric C. columna, and Anomastraea irregularis, Horastrea indica,...
Tipo: Article / Letter to the editor
Palavras-chave: Psammocora; Siderastreidae; Fungiidae; Synapticulae; Fulturae; ITS; Statistical parsimony; 42.79.
Ano: 2007
URL: http://www.repository.naturalis.nl/record/217424

A fissitunicate ascus mechanism in the Calosphaeriaceae, and novel species of Jattaea and Calosphaeria on Prunus wood
Provedor de dados: 16
Autores: Damm, U.; Crous, P.W.; Fourie, P.H.
During a survey of Prunus wood from South Africa, isolations were made of three presumably Calosphaerialean fungi that formed hyphomycetous, phialidic anamorphs in culture. In order to reveal the phylogenetic relationship of these fungi, they were characterised on a morphological and molecular (LSU and ITS rDNA) basis. Two isolates that formed a teleomorph in culture are newly described as Calosphaeria africana sp. nov. Although asci of Calosphaeria are characterised by having non-amyloid apical rings, two functional wall layers were observed in asci of C. africana, which has hitherto not been observed in any member of the Calosphaeriaceae. However, Calosphaeriaceae (Calosphaeriales, Sordariomycetes) are not closely related to other bitunicate fungi like...
Tipo: Article / Letter to the editor
Palavras-chave: Apical ring; Ascospore discharge; Ascus dehiscence; Beta-tubulin; Calosphaeria; Calosphaeriaceae; ITS; Jattaea; LSU; Phialophora; Prunus; SSU; Systematics.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/531626

Host specificity and speciation of Mycosphaerella and Teratosphaeria species associated with leaf spots of Proteaceae
Provedor de dados: 16
Autores: Crous, P.W.; Summerell, B.A.; Mostert, L.; Groenewald, J.Z.
Cheirosporium gen. nov. is characterised by the production of sporodochial conidiomata, semi-macronematous to macronematous onidiophores that possess several distinct sterile branches, and cheiroid, smooth-walled conidia with rhexolytic secession. The 28S rDNA and ITS rDNA operon of this taxon were amplified and sequenced. A BLAST search revealed low homology between Cheirosporium triseriale and existing sequences in public databases, supporting the hypothesis that the species is new to science. Phylogenetic analysis showed that C. triseriale groups with Dictyosporium and allied species, and nests within the Pleosporales (Dothideomycetes, Ascomycota).heirosporium is morphologically distinct from the cheirosporous genera Cheiromyces, Cheiromycina,...
Tipo: Article / Letter to the editor
Palavras-chave: ITS; Leucadendron; Leucospermum; Mycosphaerella; Protea; Teratosphaeria.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/532268

Stripe smuts of grasses: one lineage or high levels of polyphyly?
Provedor de dados: 16
Autores: Savchenko, K.G.; Carris, L.M.; Castlebury, L.A.; Heluta, V.P.; Wasser, S.P.; Nevo, E.
Stripe smut of grasses, Ustilago striiformis s.l., is a complex of smut fungi widely distributed over temperate and subtropical regions. The disease results in the shredding and death of leaf tissue following the rupture of elongated sori. Nearly 100 different grass species in more than 30 genera are infected by stripe smut. During the last two centuries more than 30 smut taxa have been described from members of this complex. The present study attempts to clarify the taxonomy and phylogeny of stripe smuts on grasses by analysing both morphological and molecular data. More than 200 specimens from different continents and host plants were examined. DNA was extracted from teliospores of 23 specimens from different hosts collected in Europe, Asia, and North...
Tipo: Article / Letter to the editor
Palavras-chave: Fungi; ITS; LSU; Phylogeny; Plant pathogens; Ustilaginaceae.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/531715

Stem cankers on sunflower (Helianthus annuus) in Australia reveal a complex of pathogenic Diaporthe (Phomopsis) species
Provedor de dados: 16
Autores: Thompson, S.M.; Tan, Y.P.; Young, A.J.; Neate, S.M.; Aitken, E.A.B.; Shivas, R.G.
The identification of Diaporthe (anamorph Phomopsis) species associated with stem canker of sunflower (Helianthus annuus) in Australia was studied using morphology, DNA sequence analysis and pathology. Phylogenetic analysis revealed three clades that did not correspond with known taxa, and these are believed to represent novel species. Diaporthe gulyae sp. nov. is described for isolates that caused a severe stem canker, specifically pale brown to dark brown, irregularly shaped lesions centred at the stem nodes with pith deterioration and midstem lodging. This pathogenicity of D. gulyae was confirmed by satisfying Koch’s Postulates. These symptoms are almost identical to those of sunflower stem canker caused by D. helianthi that can cause yield reductions...
Tipo: Article / Letter to the editor
Palavras-chave: Diaporthe gulyae; Diaporthe kochmanii; Diaporthe kongii; ITS; Phylogeny; Sunflower taxonomy; TEF-1α.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/531766

Niche sharing reflects a poorly understood biodiversity phenomenon
Provedor de dados: 16
Autores: Crous, P.W.; Wingfield, M.J.; Groenewald, J.Z.
Eucalyptus spp. are susceptible to a large number of foliar pathogens, some of which can cause serious defoliation and die-back. In this study, a single leaf spot on a Eucalyptus leaf collected in Madagascar revealed an unusual association of microfungi with disease symptoms. Initial observations indicated that the leaf spot was associated with Mycosphaerella marksii, a common pathogen of eucalypts. However, more intensive scrutiny showed the presence of several other microfungi co-occurring in this, and other leaf spots on the leaf. A total of 41 single conidial propagules were subsequently obtained from a single lesion for morphological study and DNA sequence comparisons. Based on these data, 11 members of the Capnodiales, including one species of...
Tipo: Article / Letter to the editor
Palavras-chave: Eucalyptus; ITS; Mycosphaerella; Systematics; Teratosphaeria.
Ano: 2009
URL: http://www.repository.naturalis.nl/record/531761

Phylogenetic and taxonomic revision of Lopadostoma
Provedor de dados: 16
Autores: Jaklitsch, W.M.; Fournier, J.; Rogers, J.D.; Voglmayr, H.
The genus Lopadostoma (Xylariaceae, Xylariales) is revised. Most species formerly assigned to Lopadostoma do not belong to the genus. Twelve species are herein recognised, of which two are only known from morphology. Ten species, of which six (L. americanum, L. fagi, L. insulare, L. lechatii, L. meridionale and L. quercicola) are newly described, are characterised by both morphology and DNA phylogeny using LSU, ITS and rpb2 sequences. Morphologically, ecologically and phylogenetically Lopadostoma is a well-defined genus comprising exclusively species with pustular pseudostroma development in bark of angiospermous trees. Phaeosperma ailanthi, Phaeosperma dryophilum and Sphaeria linosperma are combined in Lopadostoma. Lopadostoma gastrinum is neotypified and...
Tipo: Article / Letter to the editor
Palavras-chave: Anthostoma; Anthostomella; Ascomycota; Diatrypaceae; ITS; LSU; Phaeosperma; Pyrenomycetes; Rpb2; Xylariaceae; Xylariales.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/531871

Taxonomy and multi-gene phylogeny of Datronia (Polyporales, Basidiomycota
Provedor de dados: 16
Autores: Li, H.J.; Cui, B.K.; Dai, Y.C.
Taxonomic and phylogenetic studies on Datronia were carried out. Phylogeny based on ITS, nLSU and RBP2 regions revealed that Datronia in current sense includes species belonging to three distantly related clades in polypores. The Datronia in a restricted sense is proposed for the clade including the type species D. mollis and D. stereoides. Neodatronia gen. nov. was proposed for two new resupinate species, N. gaoligongensis and N. sinensis. Species of Neodatronia differ from Datronia s.s. by their resupinate basidiomes, moderately to frequently branched skeletal hyphae in subiculum. Datroniella gen. nov., typified by D. scutellata was proposed for species in the other clade. Four new species of Datroniella, D. melanocarpa, D. subtropica, D. tibetica and D....
Tipo: Article / Letter to the editor
Palavras-chave: ITS; NLSU; Polyporaceae; RPB2; Wood-inhabiting fungi.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/532030

Molecular phylogenetic analyses reveal a close evolutionary relationship between Podosphaera (Erysiphales: Erysiphaceae) and its rosaceous hosts
Provedor de dados: 16
Autores: Takamatsu, S.; Niinomi, S.; Harada, M.; Havrylenko, M.
Podosphaera is a genus of the powdery mildew fungi belonging to the tribe Cystotheceae of the Erysiphaceae. Among the host plants of Podosphaera, 86 % of hosts of the section Podosphaera and 57 % hosts of the subsection Sphaerotheca belong to the Rosaceae. In order to reconstruct the phylogeny of Podosphaera and to determine evolutionary relationships between Podosphaera and its host plants, we used 152 ITS sequences and 69 28S rDNA sequences of Podosphaera for phylogenetic analyses. As a result, Podosphaera was divided into two large clades: clade 1, consisting of the section Podosphaera on Prunus (P. tridactyla s.l.) and subsection Magnicellulatae; and clade 2, composed of the remaining member of section Podosphaera and subsection Sphaerotheca. Because...
Tipo: Article / Letter to the editor
Palavras-chave: 28S rDNA; Evolution; ITS; Molecular clock; Phylogeny; Powdery mildew fungi; Rosaceae.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/532391

Novel Curvularia species from clinical specimens
Provedor de dados: 16
Autores: Madrid, H.; da Cunha, K.C.; Gené, J.; Dijksterhuis, J.; Cano, J.; Sutton, D.A.; Guarro, J.; Crous, P.W.
The fungal genus Curvularia includes numerous plant pathogens and some emerging opportunistic pathogens of humans. In a previous study we used morphology and sequences of the nuclear ribosomal internal transcribed spacer region (ITS) and the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene to identify species within a set of 99 clinical Curvularia isolates from the USA. Seventy-two isolates could be identiﬁed while the remaining 27 isolates belonged in three unclassiﬁed clades that were tentatively labelled Curvularia sp. I, II and III. In the present study, we further assess the taxonomic placement of these isolates using sequences of ITS, gpd, the large subunit rDNA, and the second largest subunit of RNA polymerase II. DNA sequence comparisons with a...
Tipo: Article / Letter to the editor
Palavras-chave: Bipolaris; Curvularia; Gpd; ITS; LSU; Phylogeny; RPB2; Systematics.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/532180

Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype
Provedor de dados: 16
Autores: Woudenberg, J.H.C.; Aveskamp, M.M.; de Gruyter, J.; Spiers, A.G.; P.W.Crous.
The fungal pathogen Phoma clematidina is used as a biological agent to control the invasive plant species Clematis vitalba in New Zealand. Research conducted on P. clematidina as a potential biocontrol agent against,C. vitalba, led to the discovery of two perithecial-forming strains. To assess the diversity of P. clematidina and to clarify the teleomorph-anamorph relationship, phylogenetic analyses of 18 P. clematidina strains, reference strains representing the Phoma sections in the Didymellaceae and strains of related species associated with Clematis were conducted. Partial sequences of the ITS1, ITS2 and 5.8S rRNA gene, the ß-tubulin gene and 28S rRNA gene were used to clarify intra- and inter-species relationships. These analyses revealed that P....
Tipo: Article / Letter to the editor
Palavras-chave: Ascochyta vitalbae; SS-tubulin; Clematis; Didymella clematidis; Didymella vitalbina; DNA phylogeny; ITS; LSU; Taxonomy.
Ano: 2009
URL: http://www.repository.naturalis.nl/record/532396

Zymoseptoria gen. nov.: a new genus to accommodate Septoria-like species occurring on graminicolous hosts
Provedor de dados: 16
Autores: Quaedvlieg, W.; Kema, G.H.J.; Groenewald, J.Z.; Verkley, G.J.M.; Seifbarghi, S.; Razavi, M.; Mirzadi Gohari, A.; Mehrabi, R.; Crous, P.W.
The Mycosphaerella complex is both poly- and paraphyletic, containing several different families and genera. The genus Mycosphaerella is restricted to species with Ramularia anamorphs, while Septoria is restricted to taxa that cluster with the type species of Septoria, S. cytisi, being closely related to Cercospora in the Mycosphaerellaceae. Species that occur on graminicolous hosts represent an as yet undescribed genus, for which the name Zymoseptoria is proposed. Based on the 28S nrDNA phylogeny derived in this study, Zymoseptoria is shown to cluster apart from Septoria. Morphologically species of Zymoseptoria can also be distinguished by their yeast-like growth in culture, and the formation of different conidial types that are absent in Septoria s.str....
Tipo: Article / Letter to the editor
Palavras-chave: Hordeum vulgare; ITS; LSU; Multilocus sequence typing; Mycosphaerella; Septoria; Systematics; Triticum aestivum.
Ano: 2011
URL: http://www.repository.naturalis.nl/record/532144

Morphological plasticity in Cladosporium sphaerospermum
Provedor de dados: 16
Autores: Dugan, F.M.; Braun, U.; Groenewald, J.Z.; Crous, P.W.
A morphologically distinct isolate of Cladosporium sphaerospermum from a North American patent collection, referenced as Cladosporium lignicola in the patent, was examined. Generic affinity was confirmed by scanning electron microscopic examination of conidiogenous loci and conidial hila. Species identity as C. sphaerospermum was indicated by DNA sequence data derived from actin and translation elongation factor 1-α genes, and the internal transcribed spacer region. The isolate broadens the morphological limits of C. sphaerospermum by production of obclavate, occasionally transversely septate conidia with subrostrate conidiogenous apices (‘alternarioid’ conidia), and by production of conidia larger than those in prior standard descriptions. Type material...
Tipo: Article / Letter to the editor
Palavras-chave: Actin; Cladosporium Davidiella; EF1-α; ITS; Morphology; Wood-inhabiting.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/531979

Barriopsis iraniana and Phaeobotryon cupressi: two new species of the Botryosphaeriaceae from trees in Iran
Provedor de dados: 16
Autores: Abdollahzadeh, J.; Mohammadi Goltapeh, E.; Javadi, A.; Shams-bakhsh, M.; Zare, R.; Phillips:, A.J.L.
Species in the Botryosphaeriaceae are well known as pathogens and saprobes of woody hosts, but little is known about the species that occur in Iran. In a recent survey of this family in Iran two fungi with diplodia-like anamorphs were isolated from various tree hosts. These two fungi were fully characterised in terms of morphology of the anamorphs in culture, and sequences of the ITS1/ITS2 regions of the ribosomal DNA operon and partial sequences of the translation elongation factor 1-α. Phylogenetic analyses placed them within a clade consisting of Barriopsis and Phaeobotryon species, but they were clearly distinct from known species in these genera. Therefore, they are described here as two new species, namely Barriopsis iraniana on Citrus, Mangifera and...
Tipo: Article / Letter to the editor
Palavras-chave: Citrus; EF 1-α; ITS; Mangifera; Olea; Phylogeny; Systematics; Taxonomy.
Ano: 2009
URL: http://www.repository.naturalis.nl/record/532017

Global diversity and molecular systematics of Wrightoporia s.l. (Russulales, Basidiomycota)
Provedor de dados: 16
Autores: Chen, J.J.; Cui, B.K.; Dai, Y.C.
Wrightoporia accommodates polypores producing finely asperulate and amyloid basidiospores, and causing white rot. Thirty-nine species have been described or transferred to this genus; however, only a few species have been referred to molecular phylogeny. In this study, about 140 worldwide specimens of Wrightoporia s.l. were studied morphologically, and ITS and/or nLSU regions from 37 samples, representing 19 species, were sequenced for phylogenetic analysis. Six clades of Wrightoporia s.l. were recognized. The Wrightoporia s.str. clade includes W. avellanea, W. lenta (the generic type) and W. subavellanea. Three clades segregating from Wrightoporia s.str. were proposed separately as three new genera, namely Larssoniporia gen. nov., Pseudowrightoporia gen....
Tipo: Article / Letter to the editor
Palavras-chave: ITS; NLSU; Polypore; Wood-inhabiting fungi.
Ano: 2016
URL: http://www.repository.naturalis.nl/record/611851

How important are conidial appendages?
Provedor de dados: 16
Autores: Crous, P.W.; Verkley, G.J.M.; Christensen, M.; Castañeda-Ruiz, R.F.; Groenewald, J.Z.
The genus Dinemasporium is used as a case study to evaluate the importance of conidial appendages for generic level classification of coelomycetous fungi. Based on morphology and sequence data of the large subunit nuclear ribosomal RNA gene (LSU, 28S) and the internal transcribed spacers and 5.8S rRNA gene of the nr DNA operon, the genus Dinemasporium is circumscribed, and an epitype designated for D. strigosum, the type of the genus. A further five species are introduced in Dinemasporium, namely D. pseudostrigosum (isolated from Triticum aestivum, Germany and Stigmaphyllon sagraeanum, Cuba), D. americana (soil, USA), D. polygonum (Polygonum sachalinense, Netherlands), D. pseudoindicum (soil, USA), and D. morbidum (human sputum, Netherlands and hare dung,...
Tipo: Article / Letter to the editor
Palavras-chave: Chaetosphaeriaceae; Dinemasporium; ITS; LSU; Sordariomycetes; Systematics.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/531966

Two new Ophiostoma species from Protea caffra in Zambia
Provedor de dados: 16
Autores: Roets, F.; Wingfield, B.D.; Beer, Z.W. de; Wingfield, M.J.; Dreyer, L.L.
The genus Ophiostoma (Ophiostomatales) has a global distribution and species are best known for their association with bark beetles (Curculionidae: Scolytinae) on conifers. An unusual assemblage of these fungi is closely associated with the African endemic plant genus Protea (Proteaceae). Protea-associated Ophiostoma species are ecologically atypical as they colonise the fruiting structures of various serotinous Protea species. Seven species have been described from this niche in South Africa. It has been speculated that novel species may be present in other African countries where these host plants also occur. This view was corroborated by recent collections of two unknown species from Protea caffra trees in Zambia. In the present study we evaluate the...
Tipo: Article / Letter to the editor
Palavras-chave: Β-tubulin; ITS; Ophiostoma; Phylogeny; Protea; Taxonomy.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/532360

First morphogenetic identification of Fusarium solani isolated from orange fruit in Egypt
Provedor de dados: 85
Autores: Elsalam,KA; Youssef,K; Almoammar,H.
Losses due to postharvest decay may occur at any time during postharvest handling, from harvest to consumption affecting the produce quality and quantity. Accurate identification of the pathogen causing postharvest disease is essential to the selection of an appropriate disease control approach. Nine isolates of Fusarium recovered from orange fruit were identified as Fusarium solani. The fungus is involved with fruit decay. The obtained cultures were purified and grown on potato-dextrose agar (PDA), malt yeast agar (MYA), and Czapek's nutrient media (CNM) under light for identification. A pathogenicity test was carried out to fulfil Koch's postulates. The pathogen could only enter ripe orange fruit through wounds and cracks causing the rot disease. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusarium fruit rot; Orange; Pathogenicity; ITS.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000100018

Avaliação de populações de possíveis rizobactérias em solos sob espécies florestais
Provedor de dados: 90
Autores: Pereira,Rodrigo Matheus; Silveira,Érico Leandro da; Carareto-Alves,Lúcia Maria; Lemos,Eliana Gertrudes de Macedo.
Embora estudos recentes relatem a utilização de RCPC (Rizobactérias Promotoras de Crescimento de Plantas) no Brasil, raríssimos trabalhos avaliam a presença natural dessas espécies bacterianas no solo. O objetivo deste trabalho foi avaliar a ocorrência de RPCP em duas amostras de solo sob diferentes tipos de manejo, através da construção e do seqüenciamento de bibliotecas de DNA metagenômico. Utilizaram-se oligonucleotídeos específicos para amplificação da região hipervariável do espaço intergênico dos genes ribossomais 16S-23S de DNA extraído de diferentes solos, sob Eucalyptus sp. e sob mata. Os fragmentos obtidos foram inseridos em vetor e clonados. As bibliotecas geraram 495 clones, que foram seqüenciados e identificados através de comparações...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RPCP; Ecologia microbiana; Eucalyptus sp.; ITS.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832008000500013

Diversity of the marine picocyanobacteria Prochlorococcus and Synechococcus assessed by terminal restriction fragment length polymorphisms of 16S-23S rRNA internal transcribed spacer sequences
Provedor de dados: 97
Autores: LAVIN,PARIS; GÓMEZ,PATRICIA; GONZÁLE,BERNARDO; ULLOA,OSVALDO.
In order to assess the appropriateness of the use of internal transcribed spacer (ITS) sequences for the study of population genetics of marine cyanobacteria, we amplified and cloned the 16S rRNA gene plus the 16S-23S ITS regions of six strains of Prochlorococcus and Synechococcus. We analyzed them by denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphisms (T-RFLP). When using the standard application of these techniques, we obtained more than one band or terminal restriction fragment (T-RF) per strain or cloned sequence. Reports in literature have suggested that these anomalies can result from the formation of secondary structures. Secondary structures of the ITS sequences of Prochlorococcus and Synechococcus...
Tipo: Journal article
Palavras-chave: Cyanobacteria; DGGE; T-RFLP; ITS; Secondary structure.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2008000400006

Fusarium oxysporum and F. verticillioides associated with damping-off in Pinus spp.
Provedor de dados: 124
Autores: Maciel,Caciara Gonzatto; Walker,Clair; Santos,Ricardo Feliciano dos; Muniz,Marlove Fátima Brião; Brum,Daniele Lemos.
ABSTRACT Occurrence of Fusarium spp. is one of the problems, most limiting to growth of seedlings, in nurseries. This pathogen can be transmitted via seeds and causes damages to the seedlings during pre- and post-emergence stages. The present study aimed to identify Fusarium spp. at the species level based on morphological and molecular characteristics and to verify the pathogenicity of these isolates in seeds lots of Pinus elliottii and P. taeda. For this, we used two Fusarium isolates and five lots of Pinus spp. seeds. Morphological characterization was performed based on a key, specific to Fusarium spp. identification, whereas, molecular identification was carried out by amplification and sequencing of the regions from internal transcribed spacer (ITS)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ITS; Elongation factor (tef1); Pinus taeda; Pinus elliottii.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000100134

Diversidad genética de Trichoderma spp. en Venezuela, determinada mediante análisis combinado ITS-AFLP
Provedor de dados: 100
Autores: Sosa,Daynet; Pérez Martínez,Simón; Molina,Sandy; Demey,Jhonny; Gómez,Kelly; Domínguez,Diamarys; Istúriz,María; Rumbos,Raisa; Parra,Dercy.
Se estudió la diversidad genética entre 68 aislados de Trichoderma procedentes de diferentes cultivos, sustratos y localidades, mediante los análisis ITS-AFLP. Se secuenció la región ITS1-5.8S-ITS2 del ADN ribosomal amplificada con los cebadores ITS1 e ITS4 y en el caso de los AFLP se utilizaron cuatro combinaciones de oligonucleótidos. Las relaciones genéticas entre los aislados se analizaron mediante el uso combinado del Análisis de Coordenadas Principales, el Análisis de Conglomerados y el ajuste de un Biplot Logístico Externo sobre datos de disimilitud, utilizando los coeficientes de Jaccard, Emparejamiento simple, Dice y Rogers y Tanimoto. Se identificaron nueve especies y las más abundantes fueron Hypocrea lixii (anamorfoTrichoderma harzianum) y T....
Tipo: Journal article
Palavras-chave: Trichoderma; ITS; AFLP; Polimorfismo; Control biológico.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000100005

VARIABILIDAD MOLECULAR EN AISLAMIENTOS DE Phytophthora nicotianae VAN BREDA DE HAAN
Provedor de dados: 100
Autores: Peteira,Belkis; Toledo,Verónica; Martínez,B.
La pata prieta, causada por el patógeno Phytophthora nicotianae es una de las enfermedades fungosas importantes que atacan al cultivo del tabaco. Para apoyar de manera racional el programa de mejoramiento del cultivo para la obtención de variedades resistentes se hace necesario el conocimiento de la variabilidad de los aislamientos del patógeno. Las técnicas moleculares son una herramienta útil en tal sentido. Se caracterizaron a través ITS y corte de los fragmentos ITS amplificados y de RAPDs, un grupo de 50 aislamientos representativos de diferentes regiones del país, razas y características culturales. Los ITS rindieron un producto para todos los aislamientos de 850pb y la digestión de los mismos solo fue posible con la enzima MspI. A pesar de obtenerse...
Tipo: Journal article
Palavras-chave: Phytophthora nicotianae; Caracterización molecular; RAPD; ITS.
Ano: 2008
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522008000300008

Molecular identification of Aspergillus spp. isolated from coffee beans
Provedor de dados: 63
Autores: Magnani,Marciane; Fernandes,Thiago; Prete,Cássio Egidio Cavenaghi; Homechim,Martin; Ono,Elisabete Yurie Sataque; Vilas-Boas,Laurival Antonio; Sartori,Daniele; Furlaneto,Márcia Cristina; Fungaro,Maria Helena Pelegrinelli.
Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ITS; Ribosomal DNA; Ochratoxin; Mycotoxin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100009

Genotypic diversity among brazilian isolates of Macrophomina phaseolina revealed by RAPD
Provedor de dados: 102
Autores: Almeida,Álvaro M. R.; Abdelnoor,Ricardo V.; Arias,Carlos A. Arrabal; Carvalho,Valdemar P.; Jacoud Filho,David S.; Marin,Silvana R. R.; Benato,Luís C.; Pinto,Mauro C.; Carvalho,Cláudio G. P.
Macrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD; ITS; PCR-RFLP; Soybean charcoal rot.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000300009

Diversidade genética de isolados de Rhizoctonia solani coletados em feijão-caupi no Estado de Roraima
Provedor de dados: 102
Autores: Sartorato,Aloísio; Nechet,Kátia L.; Halfeld-Vieira,Bernardo A.
O feijão-caupi (Vigna unguiculata) é uma das principais fontes de proteína para a população de baixa renda, principalmente nas Regiões Norte e Nordeste do Brasil. Esta leguminosa é suscetível a várias doenças incluindo a mela ou murcha-da-teia-micélica, cujo agente causal é o fungo Rhizoctonia solani (teleomorfo: Thanatephorus cucumeris). Embora Rhizoctonia solani seja um agente causal de doença muito importante, no Brasil inexiste qualquer informação sobre as características de seus isolados associados ao feijão-caupi. O objetivo do presente estudo foi avaliar, utilizando-se das técnicas Random Amplified Polymorphic DNA (RAPD) e RFLP-ITS (Internal Transcribed Spacer), a diversidade genética de isolados de R. solani coletados de plantas de feijão-caupi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD; ITS; Vigna unguiculata; Feijão de corda; Feijão macassar; Mela; Murcha-da-teia-micélica.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582006000300009

Genetic analyses of Rhizoctonia solani isolates from Phaseolus vulgaris grown in the Atlantic Rainforest Region of São Paulo, Brazil
Provedor de dados: 102
Autores: MEINHARDT,LYNDEL W.; WULFF,NELSON A.; BELLATO,CLÁUDIA M.; TSAI,SIU M.
Rhizoctonia solani isolates obtained from common beans (Phaseolus vulgaris) grown in the mountainous Atlantic Rainforest (Mata Atlântica) region of São Paulo, Brazil, were analyzed to determine their genetic diversity using internal transcribed spacer (ITS), microsatellite and telomere sequence-based PCR primers. Restriction digestion of the ITS1/5.8S/ITS2 ribosomal regions yielded unique banding patterns specific for AG4 and its subgroups. The ITS restriction digestion (ITS/RFLP), telomere and microsatellite primers identified five to 11 genotypes within the isolates of R. solani. While all isolates were pathogenic on beans, there was no correlation found between genotypic differences and pathogenicity. The different PCR primers revealed a number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mata Atlântica; Genetic biodiversity; Soil-borne fungi; ITS; Telomere.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300004

Ceratobasidium como hongo micorrízico de orquídeas en Colombia
Provedor de dados: 109
Autores: Mosquera-Espinosa,Ana T.; Bayman,Paul; Otero,J. Túpac.
Las orquídeas se caracterizan por su relación micorrízica obligada para la germinación de las semillas. El micosimbionte es principalmente del género-forma Rhizoctonia, al igual que sus teleomorfos de los géneros Ceratobasidium, Tulasnella, Thanatephorus y Sebacina. En Colombia hasta la fecha son muy pocos los reportes sobre hongos micorrízicos de orquídeas. Para el presente estudio se planteó el aislamiento e identificación de hongos micorrízicos de algunas orquídeas de diferentes há bitats en Colombia, siguiendo la metodología de contaje de núcleos en células de hifas jóvenes y la secuenciación de la región ITS de los genes ribosomales nucleares. Se identificaron doce aislamientos provenientes de ocho plantas de especies diferentes de orquídeas. Tanto...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ceratobasidium; Rhizoctonia; Hongos endófitos; Micorríza en orquídeas; ITS; Orchidaceae.
Ano: 2010
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122010000300007

Identificación molecular de aislamientos de Moniliophthora roreri en huertos de cacao de Norte de Santander, Colombia
Provedor de dados: 109
Autores: Suárez Contreras,Liliana Yanet.
El hongo fitopatógeno Moniliophthora roreri produce la moniliasis, una enfermedad que destruye la mazorca de cacao (Theobroma cacao L.) y, por tanto, reduce la producción del cultivo y los ingresos de los agricultores. En el departamento de Norte de Santander, Colombia, las pérdidas por esta enfermedad pueden alcanzar hasta 60% de la producción. El conocimiento sobre la biología del hongo es escaso y actualmente se están realizando estudios de identificación macroscópica, microscópica, y comportamiento in vitro frente a antagonistas como: Trichoderma asperellum, T. longibrachiatum, Paecilomyces sp. y Bacillus brevis. En ocho municipios y corregimientos del departamento (El Zulia, Cúcuta, Tibú, Sardinata, Bucarasica, Teorama, Agua clara y El Tarra) se...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moniliophthora roreri; Theobroma cacao; Moniliasis; ITS.
Ano: 2016
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122016000100008

DIVERSIDAD GENÉTICA DE Peronospora sparsa (PERONOSPORACEAE) EN CULTIVOS DE ROSA DE COLOMBIA
Provedor de dados: 37
Autores: AYALA-VÁSQUEZ,MARILUZ; ARGEL-ROLDAN,LUZ EDITH; JARAMILLO-VILLEGAS,SONIA; MARÍN-MONTOYA,MAURICIO.
El mildeo velloso de la rosa es la enfermedad más limitante de este cultivo en Colombia . Aunque algunos trabajos de investigación se han adelantado recientemente sobre la epidemiología de esta enfermedad, uno de los campos menos estudiados es el nivel de variabilidad de su agente causal. El conocimiento de este aspecto es fundamental para definir las estrategias de manejo, especialmente aquellas relacionadas con la resistencia genética y el control químico. Los objetivos de esta investigación fueron confirmar la identidad taxonómica del agente causal del mildeo velloso de la rosa y profundizar en el conocimiento de su estructura poblacional en Colombia . Para esto se colectaron 34 aislamientos del pseudohongo en cultivos de rosas ubicados en Antioquia y...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADNr; Flores; ITS; Marcadores moleculares; Mildeo velloso; Variabilidad genética.
Ano: 2008
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2008000100005

Isolation and molecular characterization of Rhizoctonia-like fungi associated with orchid roots in the Quadrilátero Ferrífero and Zona da Mata regions of the state of Minas Gerais, Brazil
Provedor de dados: 39
Autores: Nogueira,Ricardo Eustáquio; Berg,Cassio van den; Pereira,Olinto Liparini; Kasuya,Maria Catarina Megumi.
Mycorrhizal associations can be considered required for orchids, which depend on the fungi for germination and establishment in natural conditions. Knowledge of the mycorrhizal fungi is important for programs aimed at the reintroduction, conservation and management of orchid species. The objective of this study was the molecular characterization of Rhizoctonia-like fungi from orchids in the Quadrilátero Ferrífero ("Iron Quadrangle") and Zona da Mata ("Forest Zone") regions of the state of Minas Gerais, Brazil. The affinities of these fungi were studied by comparing the rRNA internal transcribed spacer region with that of other isolates and sequences in GenBank. Three isolates had an affinity for Epulorhiza repens, and one was the holotypeof E. epiphytica.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Epulorhiza repens; E. epiphytica; ITS; Tulasnella calospora; Orchidaceae; Mycorrhiza.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200017

Análise filogenética de Phytophthora capsici Leonian do Estado de São Paulo baseada na seqüência de nucleotídeos da região ITS-5.8S rDNA - DOI: 10.4025/actascibiolsci.v25i2.2033
Provedor de dados: 3
Autores: Rosa, Daniel Dias; UNESP; Marque, Janaina Marianno de; UNESP; Fenille, Roseli Chela; Laboratorio de Apoio Vegetal - MAPA; Souza, Nilton Luiz de; UNESP; Kuramae, Eiko Eurya; Fungal Biodiversity Center.
Análise filogenética através de características morfológicas e culturais é considerada inconsistente e de difícil aceitação cientiífica, uma vez que não há como estabelecer a direção da evolução de caracteres morfológicos. Os mMarcadores moleculares são mais apropriados para análises filogenéticas. O seqüeênciamento das regiões ITS1 e ITS2 (Internal Transcribed Spacer) e o gene 5.8S rDNA forami utilizados para estimar a variação e a distância genética entre 10 isolados de Phytophthora capsici. A quantidade de variação genética entre os isolados de P. capsici de diferentes regiões do estadoEstado de São Paulo foi de 0,1% a 1,6% e de 0,1% a 1,1% nas regiões ITS1 e ITS2, respectivamente, e uma distância filogenética de 78,5% entre os isolados de P. capsici e...
Palavras-chave: 2.01.00.00-0    Biologia Geral DNA ribossomal; ITS; FFilogenia; Phytophthora sp 2.01.00.00-0    Biologia Geral.
Ano: 2003
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2033

Polymerase chain reaction (PCR) identification of terverticillate Penicillium species isolated from agricultural soils in eskişehir province
Provedor de dados: 52
Autores: Demirel,Rasime; Sariozlu,Nalan Yilmaz; İlhan,Semra.
In the present study, nine terverticillate Penicillium isolates (P. griseofulfum, P. puberulum, P. crustosum, P. aurantiogriseum, P. chrysogenum, P. primulinum, P. expansum, P. viridicatum, Eupenicillium egyptiacum) from 56 soil samples were characterized genetically by a PCR method. The DNAs of the strains were isolated using the glass beads and vortexing extraction method and then used for PCR amplification with the internal transcribed spacer 1 (ITS1) and ITS4 universal fungal specific primers. The ITS regions of fungal ribosomal DNA (rDNA) were sequenced through the CEQ 8000 Genetic Analysis System. ITS-5.8S sequences obtained were compared with those deposited in the GenBank Database. The results indicated that the identification of Penicillium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillium; Identification methods; PCR; ITS.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600013

Pandan (Pandanus sp), Rotan (Calamus sp), and Rengas (Gluta sp) from Kajuik Lake, Riau Province, Indonesia
Provedor de dados: 52
Autores: Roslim,Dewi Indriyani.
ABSTRACT Pandan (Pandanus sp), Rotan (Calamus sp), and Rengas (Gluta sp) are the three most important plants growing at Kajuik Lake, Langgam, Riau Province, Indonesia; however, their species names have not been identified. This study aimed to identify their species names using nuclear internal transcribed spacer (ITS) and psbA-trnH intergenic spacer sequences. The method employed was DNA isolation from fresh leaves, PCR using primer pairs of ITS region for Pandanus sp and psbA-trnH intergenic spacer for Calamus sp and Gluta sp, electrophoresis, sequencing, and data analysis using BLASTn program and MEGA software version 6.0. Pandanus tectorius was the only one accession that was similar to Pandanus sp with the identity was 90%, however the query cover was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calamus sp; Gluta sp; ITS; Pandanus sp; PsbA-trnH intergenic spacer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100408

Screening of endophytic fungi stored in a culture collection for taxol production
Provedor de dados: 58
Autores: Andrade,Hortência Farias de; Araújo,Lívia Caroline Alexandre de; Santos,Bruno Souza dos; Paiva,Patrícia Maria Guedes; Napoleão,Thiago Henrique; Correia,Maria Tereza dos Santos; Oliveira,Maria Betânia Melo de; Lima,Gláucia Manoella de Souza; Ximenes,Rafael Matos; Silva,Túlio Diego da; Silva,Girliane Regina da; Silva,Márcia Vanusa da.
Abstract In this work, four isolates of endophytic fungi (Alternaria alternata, Colletotrichum gloesporioides, Glomerella cingulata and Nigrospora sphaerica), deposited in the culture collection ‘University Recife Mycologia’ (URM) at the Universidade Federal de Pernambuco, were characterized for the genes ITS 1 and 4 (region 5.8 S) and evaluated for taxol production.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Paclitaxel; Taxol route genes; ITS; Colletotrichum gloesporioides.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500059

Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal
Provedor de dados: 58
Autores: Ragonezi,Carla; Caldeira,A. Teresa; Rosário Martins,M.; Salvador,Cátia; Santos-Silva,Celeste; Ganhão,Elsa; Klimaszewska,Krystyna; Zavattieri,Amely.
Stone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (Scop.) Rauschert and Lactarius deliciosus (L. ex Fr.) S.F. Gray fungi, were collected, pure cultured and used in in vitro co-culture with stone pine microshoots. Samples of P. arhizus and L. deliciosus for the in vitro co-cultures were collected from the pine stands southwest Portugal. The in situ characterization was based on their morphotypes. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pisolithus arhizus; Lactarius deliciosus; Pinus pinea; M13-PCR; ITS.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200047

Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits
Provedor de dados: 65
Autores: Barbosa,Marcílio Souza; Barbosa,Suzanne Nunes; Nagamoto,Nilson Satoru; Forti,Luiz Carlos; lmeida,Cícero.
ABSTRACT: Atta sexdens rubropilosa (leaf-cutter ants) has a symbiotic association with a fungus and has a negative interaction with other fungi due to parasitism of the fungus cultivated by ants; also, there are several other fungi with no exact known role occurring in their cultivated fungus garden. In the present study, we use the ITS region (internal transcribed spacer) to identify fungi in colonies treated with toxic baits. Experiments using two toxic baits were carried out: 0.75g of sulfluramid [0.3%] and 0.75g fipronil [0.003%]. Samples of fungi were collected and cultured in Czapek medium for seven days to allow fungal growth and subsequent identification. Total DNA was isolated from 100-150 mg of mycelium using the CTAB method and using PCR, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ITS; DNA barcoding; Leaf-cutter; Ants.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500150

Identificação e variabilidade genética de isolados de Colletotrichum causando antracnose em inflorescências de plantas ornamentais tropicais
Provedor de dados: 65
Autores: Barguil,Beatriz Meireles; Oliveira,Sônia Maria Alves de; Coelho,Rildo Sartori Barbosa; Beserra Júnior,José Evando Aguiar.
A antracnose afeta a qualidade de inflorescências de plantas ornamentais tropicais, e a espécie fúngica Colletotrichum gloeosporioides tem sido relacionada a essa doença apenas por análises morfológicas. Por isso, o presente trabalho teve como objetivos identificar isolados de Colletotrichum coletados em plantas de antúrio (Anthurium andraeanum), bastão do imperador (Etlingera elatior) e helicônia (Heliconia spp.), por meio de caracteres morfológicos e reação em cadeia da polimerase (PCR), e avaliar a variabilidade genética por meio de oligonucleotídeos arbitrários (AP-PCR). Pelas características morfológicas de tamanho de conídio e de apressório, todos os isolados foram identificados como C. gloeosporioides. Um fragmento de 450pb específico para C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthurium andraeanum; AP-PCR; Etlingera elatior; Heliconia spp.; ITS.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000600002