Consulta: subjectFacets:"Immobilization"
Registros recuperados: 54
Data/hora: 05/06/2024 04:32:55

Natural carriers in bioremediation: A review
Provedor de dados: Electronic Journal of Biotechnology
Autores: Dzionek,Anna; Wojcieszy&amp;#324;ska,Danuta; Guzik,Urszula.
Bioremediation of contaminated groundwater or soil is currently the cheapest and the least harmful method of removing xenobiotics from the environment. Immobilization of microorganisms capable of degrading specific contaminants significantly promotes bioremediation processes, reduces their costs, and also allows for the multiple use of biocatalysts. Among the developed methods of immobilization, adsorption on the surface is the most common method in bioremediation, due to the simplicity of the procedure and its non-toxicity. The choice of carrier is an essential element for successful bioremediation. It is also important to consider the type of process ( n s tu or ex s tu), type of pollution, and properties of immobilized microorganisms. For these reasons,...
Tipo: Journal article
Palavras-chave: Adsorption; Bioethanol production; Contamination; Heavy metals; Immobilization; Immobilized microorganisms; Natural carriers; Wine alterations; Wood barrels; Xenobiotics.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500008

Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843
Provedor de dados: Electronic Journal of Biotechnology
Autores: Riansa-ngawong,Wiboon; Suwansaard,Maneewan; Prasertsan,Poonsuk.
Palm pressed fiber (PPF) is a clean and renewable lignocellulosic material. The PPF and delignified PPF (DPPF) were used as a carrier for immobilization of Candida shehatae TISTR5843 in bioethanol production. PPF was pre-treated by milling to obtain small particles, whereas DPPF was the delignification of PPF using NaClO2. C. shehatae TISTR5843 was grown in modified yeast extract- malt (YM) medium at 30 ± 2ºC on an orbital shaker at 150 rpm for batch and repeated batch fermentation. In the batch system, immobilized cells on a small size, less than 0.5 mm, of DPPF (sDPPF) gave the maximum ethanol production of 11.5 g L-1 at 24 hrs cultivation period. The ethanol concentration and ethanol yield of sDPPF were 6.2% and 6.8% higher (ethanol production 11.5 g...
Tipo: Journal article
Palavras-chave: Candida shehatae; Ethanol production; Immobilization; Palm pressed fiber.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600001

Preparation and characterization of &amp;#954;-carrageenase immobilized onto magnetic iron oxide nanoparticles
Provedor de dados: Electronic Journal of Biotechnology
Autores: Xiao,Anfeng; Xu,Caiyun; Lin,Yan; Ni,Hui; Zhu,Yanbing; Cai,Huinong.
Background Carboxyl-functionalized magnetic nanoparticles were synthesized via chemical co-precipitation method and modified with oleic acid which was oxidized by potassium permanganate, and &amp;#954;-carrageenase from Pseudoalteromonas sp. ASY5 was subsequently immobilized onto them. The immobilization conditions were further optimized, and the characterizations of the immobilized &amp;#954;-carrageenase were investigated. Results The &amp;#954;-carrageenase was immobilized onto magnetic iron oxide nanoparticles, and the bonding was verified by Fourier transform infrared spectroscopy. The optimal conditions for &amp;#954;-carrageenase immobilization were 2.5% (w/v) glutaraldehyde, 13.9 U &amp;#954;-carrageenase for 20 mg of magnetic nanoparticles, a 2-h...
Tipo: Journal article
Palavras-chave: Carrageenase; Characterization; Immobilization; Nanoparticles.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100001

Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology
Provedor de dados: Electronic Journal of Biotechnology
Autores: Zhang,Li-Jing; Zheng,Â· Xi; Jin,Â· Zhi-Hua; Hu,Â· Sheng; He,Â· Mei-Ru.
Response surface methodology was used to optimize the fermentation conditions for the production of pristinamycin by immobilization of Streptomyces pristinaespiralis F213 in shaking &amp;#64258;ask cultivation. Seed medium volume, fermentation medium volume and shaking speed of seed culture were found to have signi&amp;#64257;cant effects on pristinamycin production by the Plackett-Burman design. The steepest ascent method was adopted to approach the vicinity of optimum space, followed by central composite design for further optimization. A quadratic model was built to &amp;#64257;t the pristinamycin production. The optimum conditions were found to be seed medium volume of 29.5 ml, fermentation medium volume of 28.8 ml, and shaking speed of seed culture at...
Tipo: Journal article
Palavras-chave: Fermentation conditions; Immobilization; Pristinamycin; Response surface methodology; Streptomyces pristinaespiralis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400006

Cyclodextrin glucanotransferase immobilization onto functionalized magnetic double mesoporous core-shell silica nanospheres
Provedor de dados: Electronic Journal of Biotechnology
Autores: Ibrahim,Abdelnasser S.S.; Al-Salamah,Ali A; El-Toni,Ahmed Mohamed; El-Tayeb,Mohamed A; Elbadawi,Yahya B.
Background Cyclodextrin glucanotransferase (CGTase) from Amphibacillus sp. NPST-10 was covalently immobilized onto amino-functionalized magnetic double mesoporous core-shell silica nanospheres (mag@d-SiO2@m-SiO2-NH2), and the properties of the immobilized enzyme were investigated. The synthesis process of the nanospheres included preparing core magnetic magnetite (Fe3O4) nanoparticles, coating the Fe3O4 with a dense silica layer, followed by further coating with functionalized or non-functionalized mesoporous silica shell. The structure of the synthesized nanospheres was characterized using TEM, XRD, and FT-IR analyses. CGTase was immobilized onto the functionalized and non-functionalized nanospheres by covalent attachment and physical adsorption. Results...
Tipo: Journal article
Palavras-chave: Amphibacillus sp; Cyclodextrin glucanotransferase; Cyclodextrins; Double mesoporous core-shell silica nanospheres; Immobilization.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200001

Kinetic analysis and effect of culture medium and coating materials during free and immobilized cell cultures of Bifidobacterium animalis subsp. lactis Bb 12
Provedor de dados: Electronic Journal of Biotechnology
Autores: Jalili,Hasan; Razavi,Hadi; Safari,Mohammad; Amrane,Abdeltif.
Microencapsulation technique appears helpful for more protection of Bifidobacteria against acid inhibitory effect. The effect of medium composition and product inhibitory in free cell culture, as well as the effect of the coating materials in immobilized cells, on biomass growth, acid production and substrate utilization kinetics of Bifidobacterium animalis subsp. lactis Bb 12 in uncontrolled batch fermentation was examined. The Monod and the Luedeking and Piret equations with a product inhibition term involving toxic power terms improved model efficiency for both growth and production. The model showed that media and coating materials had an effect on toxic power terms. Cell immobilization had a positive impact on B. animalis culture. Kinetic analysis...
Tipo: Journal article
Palavras-chave: Bifidobacterium; Immobilization; Kinetic models; Medium composition.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300002

Immobilization and stability studies of a lipase from thermophilic Bacillus sp: The effect of process parameters on immobilization of enzyme
Provedor de dados: Electronic Journal of Biotechnology
Autores: Nawani,Neerupma; Singh,Rajvinder; Kaur,Jagdeep.
A thermostable lipase was partially purified from the culture supernatant of a thermophilic Bacillus sp. The enzyme is optimally active at 60&amp;ordm;C and pH 8.0. The enzyme showed enhancement in activity in presence of benzene or hexane (30% v/v each). The activity (assayed by determining the release of pNP from pNP laurate) was stimulated up to 60% of these solvents in enzyme reaction mixture. The catalytic properties of this thermostable enzyme can be further improved via the use of different immobilization techniques and reaction conditions. Enzyme was immobilized on different solid supports and their enzyme activity and stability was compared. The enzyme was adsorbed on silica and HP-20 beads followed by cross-linking with gluteraldehyde on HP-20,...
Tipo: Journal article
Palavras-chave: Esterification; Immobilization; Lipase; Thermostability.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500011

The whole-cell immobilization of D-hydantoinase-engineered Escherichia coli for D-CpHPG biosynthesis
Provedor de dados: Electronic Journal of Biotechnology
Autores: Jin,Yuan-yuan; Li,Ya-dong; Sun,Wan; Fan,Shuai; Feng,Xiao-zhou; Wang,Kang-you; He,Wei-qing; Yang,Zhao-yong.
Background: D-Hydroxyphenylglycine is considered to be an important chiral molecular building-block of antibiotic reagents such as pesticides, and &amp;#946;-lactam antibiotics. The process of its production is catalyzed by D-hydantoinase and D-carbamoylase in a two-step enzyme reaction. How to enhance the catalytic potential of the two enzymes is valuable for industrial application. In this investigation, an Escherichia coli strain genetically engineered with D-hydantoinase was immobilized by calcium alginate with certain adjuncts to evaluate the optimal condition for the biosynthesis of D-carbamoyl-p-hydroxyphenylglycine (D-CpHPG), the compound further be converted to D-hydroxyphenylglycine (D-HPG) by carbamoylase. Results: The optimal medium to produce...
Tipo: Journal article
Palavras-chave: Calcium alginate; D-Carbamoyl-p-hydroxyphenylglycine; D-Hydantoinase; D-Hydroxyphenylglycine; Immobilization; Whole cell.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300004

Immobilization of cyclodextrin glucanotransferase on aminopropyl-functionalized silica-coated superparamagnetic nanoparticles
Provedor de dados: Electronic Journal of Biotechnology
Autores: Ibrahim,Abdelnasser S.S.; Al-Salamah,Ali A; El-Toni,Ahmed Mohamed; El-Tayeb,Mohamed A; Elbadawi,Yahya B.
Background: Cyclodextrin glycosyltransferase (CGTase) from Amphibacillus sp. NPST-10 was successfully covalently immobilized on aminopropyl-functionalized silica coated superparamagnetic nanoparticles; and the properties of immobilized enzyme were investigated. The synthesis process included preparing of core magnetic magnetite (Fe3O4) nanoparticles using solvothermal synthesis; followed by coating of Fe3O4 nanoparticles with dense amino-functionalized silica (NH2-SiO2) layer using in situ functionalization method. The structure of synthesized Fe3O4@NH2-SiO2 nanoparticles was characterized using TEM, XRD, and FT-IR analysis. Fe3O4@NH2-SiO2 nanoparticles were further activated by gluteraaldehyde as bifunctional cross linker, and the activated nanoparticles...
Tipo: Journal article
Palavras-chave: Amphibacillus sp. NPST-10; Cyclodextrin glucanotransferase; Immobilization; Magnetic nanoparticle; Silica.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600010

Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Landreau, M.; Duthoit, Frederique; Claeys-bruno, M.; Vandenabeele-trambouze, O.; Aubry, T.; Godfroy, Anne; Le Blay, Gwenaelle.
Aims  The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results  The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (&lt;0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect...
Tipo: Text
Palavras-chave: (hyper)thermophilic marine micro-organisms; Anaerobiosis; Entrapment; Gellan; Immobilization; Xanthan.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00332/44352/44000.pdf

Cultivation of an immobilized (hyper)thermophilic marine microbial community in a bioreactor
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Landreau, M.; Duthoit, Frederique; Roussel, Erwan; Schonherr, S.; Georges, Myriam; Godfroy, Anne; Le Blay, G.
Cultivation in a bioreactor of immobilized deep-sea hydrothermal microbial community was tested in order to assess the stability and reactivity of this new system. A community composed of 8 hydrothermal strains was entrapped in a polymer matrix that was used to inoculate a continuous culture in a gas-lift bioreactor. The continuous culture was performed for 41 days at successively 60°C, 55°C, 60°C, 85°C and 60°C, at pH 6.5, in anaerobic condition and constant dilution rate. Oxic stress and pH variations were tested at the beginning of the incubation. Despite these detrimental conditions, 3 strains including 2 strict anaerobes were maintained in the bioreactor. High cell concentrations (3 × 108 cells mL−1) and high ATP contents were measured in both liquid...
Tipo: Text
Palavras-chave: Immobilization; Anaerobiosis; Marine (hyper)thermophiles; Continuous culture.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00347/45851/45516.pdf

45-and 70-base DNA supramolecular polymerizations on quartz crystal microbalance biosensor
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Antoine, Elisabeth; Compere, Chantal.
Supramolecular polymerizations of 45- and 70- base DNAs on the surface of an in-situ time-resolved 27 MHz quartz crystal microbalance biosensor.
Tipo: Text
Palavras-chave: Sensor; Design; Kinetics; Immobilization; Hybridization.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1710.pdf

Utilización de la κ-carragenina como soporte de inmovilización de microorganismos marinos degradadores de hidrocarburos.
Provedor de dados: Ocean Docs: e-repository of  publications
Autores: Fonseca, E. L.; Núñez, R. R.; Villaverde, M. J; Barbán, O. M.; Ferrer, O. B.; Ramos, I.; Valdés, O.
Una marea negra provocada por el vertido deliberado o accidental de crudos de petróleo, aceite combustible, fangos o residuos petrolíferos y productos refinados, provoca un gran impacto sobre los ecosistemas marinos y costeros. En este trabajo, se desarrolla un producto compuesto por bacterias marinas inmovilizadas por atrapamiento en k- carragenina semirrefinada y en cuya formulación no se incluyen nutrientes. Los resultados muestran que la variante de inmovilización más ventajosa se obtuvo cuando fue empleada la κ-carragenina al 1% gelificada con una solución de KCL 1.5 M. Mediante análisis de Cromatografía Gaseosa de Alta Resolución y de espectroscopia (FTIR), se demostró la alta capacidad de degradación del bioproducto frente a petróleo del Yacimiento...
Tipo: Preprint
Palavras-chave: Immobilization; Oil; Immobilization; Http://aims.fao.org/aos/agrovoc/c_27550.
Ano: 2006
URL: http://hdl.handle.net/1834/3662

Maize response to nitrogen fertilization timing in two tillage systems in a soil with high organic matter content
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Sangoi,Luis; Ernani,Paulo Roberto; Silva,Paulo Regis Ferreira da.
No-tillage systems, associated to black oat as preceding cover crop, have been increasingly adopted. This has motivated anticipated maize nitrogen fertilization, transferring it from the side-dress system at the stage when plants have five to six expanded leaves to when the preceding cover crop is eliminated or to maize sowing. This study was conducted to evaluate the effects of soil tillage system and timing of N fertilization on maize grain yield and agronomic efficiency of N applied to a soil with high organic matter content. A three-year field experiment was conducted in Lages, state of Santa Catarina, from 1999 onwards. Treatments were set up in a split plot arrangement. Two soil tillage systems were tested in the main plots: conventional tillage (CT)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Nitrogen; Immobilization; Leaching; Grain yield.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832007000300011

Amplitude no fósforo microbiano em um Argissolo em pastagem nativa submetida à roçada e à introdução de espécies forrageiras com fertilização fosfatada em diferentes épocas - DOI: 10.4025/actasciagron.v30i4.5319
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Santos, Danilo Rheinheimer; UFSM; Martinazzo, Rosane; UFRGS; Gatiboni, Luciano Colpo; UDESC; Kaminski, João; UFSM; Silva, Leandro Souza da; UFSM.
Em solos com elevado grau de intemperismo, a imobilização temporária de fósforo pela biomassa microbiana do solo (BMS) pode ser um mecanismo de retardamento das reações de adsorção. O objetivo do presente trabalho foi avaliar a amplitude no fósforo microbiano (Pm) em um Argissolo em pastagem nativa submetida à roçada, introdução de forrageiras exóticas e adubadas com fertilizantes fosfatados. O estudo foi desenvolvido a partir de um experimento instalado em 1997 que constava de cinco tratamentos de fosfato e calagem (testemunha sem fósforo; hiperfosfato de gafsa; superfosfato triplo; superfosfato triplo + calcário e superfosfato simples + calcário), distribuídos em quatro blocos ao acaso. Em junho de 2002, a pastagem nativa remanescente foi roçada, semeada...
Palavras-chave: 5.01.00.00-9    Agronomia biomassa microbiana; Imobilização; Adubos fosfatados; Pastagens nativas; Disponibilidade de fósforo. microbial biomass; Immobilization; Phosphate fertilizers; Natural pasture; Phosphorus availability.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5319

Production of ethanol by Saccharomyces cerevisiae and Zymomonas mobilis Coimmobilized: proposal for the use of organic waste
Provedor de dados: Agrociencia
Autores: Ruiz-Marín,Alejandro; Canedo-López,Yunuén; Narváez-García,Asteria; Robles-Heredia,J. Carlos.
Abstract: Fermentation technologies were developed to improve the production of ethanol and an alternative is the immobilization technology, which offers advantages in comparison with free cells culture, such as the possibility of efficiently incorporating symbiotic bacteria in the same matrix. The aim of this study was to evaluate, in equivalent medium, the use of mango (Mangifera indica) waste to produce ethanol. The study compared the production of ethanol by using Zymomonas mobilis and Saccharomyces cerevisiae in free cells culture, immobilized and coimmobilized in calcium alginate in a culture medium equivalent to mango juice. It also included an evaluation of the effect of glucose concentration on productivity in a coimmobilized system. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangifera indica; Immobilization; Coimmobilization; Ethanol; Zymomonas mobilis; Saccharomyces cerevisiae.
Ano: 2016
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952016000500551

Advances in enzyme bioelectrochemistry
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: PEREIRA,ANDRESSA R.; SEDENHO,GRAZIELA C.; SOUZA,JOÃO C. P. DE; CRESPILHO,FRANK N.
ABSTRACT Bioelectrochemistry can be defined as a branch of Chemical Science concerned with electron-proton transfer and transport involving biomolecules, as well as electrode reactions of redox enzymes. The bioelectrochemical reactions and system have direct impact in biotechnological development, in medical devices designing, in the behavior of DNA-protein complexes, in green-energy and bioenergy concepts, and make it possible an understanding of metabolism of all living organisms (e.g. humans) where biomolecules are integral to health and proper functioning. In the last years, many researchers have dedicated itself to study different redox enzymes by using electrochemistry, aiming to understand their mechanisms and to develop promising bioanodes and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioelectrocatalysis; Biofuel cells; Bioelectrochemistry; Immobilization; Protein-electrode interactions; Redox enzymes.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200825

Magnetic Parkia pendula seed gum as matrix for Concanavalin A lectin immobilization and its application in affinity purification
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: RÊGO,MOACYR J.B.M.; ALMEIDA,SINARA M.; BEZERRA,SÉRGIO A.; CARVALHO JÚNIOR,LUIZ B.; BELTRÃO,EDUARDO I.C.
The present work aimed to magnetize Parkia pendula seeds gum and use it as a matrix for Concanavalin A covalent immobilization. This composite was applied in affinity purification of glycoconjugates. Parkia pendula seeds were hydrated and the gum provenient from the supernatant was precipitated and washed with ethanol and dried. The gum was magnetized in co-precipitation using solutions of Fe+2 and Fe+3. Matrix activation was accomplished with NaIO4. Magnetized Parkia pendula seeds gum with covalently immobilized Concanavalin A was used as an affinity matrix for the recognition of bovine serum fetuin glycoprotein. Fetuin elution was carried out with a solution of glucose (300mM) and evaluated through SDS-PAGE. The efficiency of lectin immobilization and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Magnetization; Parkia pendula seed gum; Concanavalin A.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301351

Adsorption, immobilization and activity of cellulase in soil: the impacts of maize straw and its humification
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Sinegani,Ali Akbar Safari; Sinegani,Mahboobe Safari.
The present work aimed to study some aspects of sorption and immobilization of cellulase molecules on soil components by the analysis of the reactions of cellulase in a soil treated with different levels of maize residue and incubated for 90 days. The analysis of variance showed that the effects of the treatments of maize straw, incubation time and their interaction on cellulase adsorption, desorption and immobilization were statistically significant. The adsorption and immobilization capacities of soil by application of maize straw increased significantly. However they decreased with decreasing the soil organic matter (SOM) after 45 days of incubation. The desorption of adsorbed cellulase molecules from the soil by washing with distilled water depended on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maize straw; Humification; Cellulase; Sorption; Immobilization; Activity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600001

Metabolic and morphometric alterations inherent to neuromuscular electric stimulation in the antagonist muscle submitted to ankle joint immobilization
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Durigan,João Luiz Quagliotti; Cancelliero,Karina Maria; Bosi,Paula Lima; Delfino,Gabriel Borges; Montebelo,Maria Imaculada de Lima; Guirro,Rinaldo Roberto de Jesus; Silva,Carlos Alberto da; Polacow,Maria Luiza Ozores.
The aim of this work was to investigate the effect of the neuromuscular electrical stimulation (ES) on the metabolic and morfometric profile of the tibialis anterior muscle, antagonist to the soleus muscle which was stimulate, under the joint immobilization condition of the ankle for 7 and 15 days. The immobilization promoted the reduction in the muscle mass (I7: 17.36%, I15: 20.83%), in the glycogen content (I7: 48%, I15: 48%), in the muscle fibers areas (I7: 27%, I15: 40%) and increase in intramuscular connective tissue density (I7: 122%, I15: 206%). The EE didn't promote significant alterations in the mass of the immobilized groups, however, promoted increase in the glycogen (IEE7: 31.25%; IEE15: 56.25%), reduction in the muscle fibers areas (IEE7: 14%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Muscle disuse; Metabolic; Morphometric; Neuromuscular electrical stimulation; Rehabilitation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100011

Immobilization of Beauveria bassiana Lipase on Silica Gel by Physical Adsorption
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Sugahara,Vanessa Hitomi; Varéa,Geni da Silva.
Extracellular lipase from Beauveria bassianastrain CG481 was immobilized by using thirteen different immobilization protocols. Silica gel was chosen as the most suitable adsorbent with 94.8% of activity yield. The adsorption on silica gel did not change the optimum pH (8.5) and temperature (45ºC) values of the free lipase (FL) for lipolytic activity, and it showed higher activities in extreme conditions (pH 9.0 to 10.5, 60ºC). The lipase immobilized on silica gel (ILS) showed enhanced stability at pH 7.0 after 120 h incubation (69.0%) when compared to FL (33.3%). The thermal stability was also enhanced by immobilization at 60ºC in aqueous (64.6%) and organic medium (95.1%), while FL showed only 40.6% of residual activity in aqueous medium and exhibited no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Lipase; Beauveria bassiana; Silica gel.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600842

Role of Glutaraldehyde in Imparting Stability to Immobilized β-Galactosidase Systems
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Satar,Rukhsana; Jafri,Mohammad Alam; Rasool,Mahmood; Ansari,Shakeel Ahmed.
ABSTRACT This review article highlights the role of glutaraldehyde as a matrix activator/stabilizer in imparting higher operational and thermal stability to β-galactosidase (βG) for biotechnological applications. Glutaraldehyde has been used extensively as a crosslinking agent as well as for functionalization of matrices to immobilize β-galactosidase. Immobilized β-galactosidase systems (IβGS) obtained as a result of glutaraldehyde treatment has been employed to hydrolyze whey and milk lactose in batch reactors, continuous packed-bed and fluidized bed reactors under various operational conditions. Moreover, these IβGS have also been utilized for the production of galactooligosaccharides in food, dairy and fermentation industries. It was observed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-galactosidase; Crosslinking; Glutaraldehyde; Immobilization; Stability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100447

Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Marques,Leila Larisa Medeiros; Buzato,João Batista; Celligoi,Maria Antonia Pedrine Colabone.
This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Invertase; Luffa cylindrica; Raffinose; Saccharomyces cerevisiae; Ultrasound.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700003

A comparative study on fungal laccases immobilized on chitosan
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Araújo,José Hilton Bernardino de; Uemura,Vinicius Oliveira; Moraes,Flavio Faria de; Barbosa,Aneli de Melo; Zanin,Gisella Maria.
The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laccases; Immobilization; Chitosan.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001

Immobilization of the Magnetic Nanoparticles with Alkaline Protease Enzyme Produced by Enterococcus hirae and Pseudomonas aeruginosa Isolated from Dairy Effluents
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Masi,Chandran; Chandramohan,C.; Ahmed,M.Fazil.
ABSTRACT Protease is an enzyme which has a wide range of applications in various fields. Extracellular protease was produced from Pseudomonas aeruginosa and Enterococcus hirae which were isolated from the effluents of diary industries. Protease immobilized with super paramagnetic nanoparticles was characterized by DLS, XRD and TEM methods in relation to their size and structure. The protease enzyme was bound to magnetic nanoparticles via surface transformation technique including Silica coated magnetic Nano composite, amine and cysteine functioned Nano composite formation. Successful binding of protease onto the particles was confirmed by TEM imaging. The maximal enzyme activity of immobilized protease was determined using universal protease assay and was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease; Super paramagnetic Nanoparticle; Nano composite; Immobilization; Activity and Stability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100462

Use of Coffee Capsules as Support Material in Upflow Anaerobic Fixed Bed Reactors
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Souza,Gabriela Rezende de; Oliveira,Luiz Fernando Coutinho de; Bello,Italoema Pinheiro; Siniscalchi,Luciene Alves Batista; Fia,Ronaldo; Gandia,Rômulo Marçal.
Abstract The aim of this work is to evaluate the performance of upflow anaerobic fixed bed reactors filled with espresso coffee capsules to treat sanitary sewage. Three reactors (R1, R2 and R3) were constructed in blue PVC pipes measuring 30 cm height and 150 mm diameter and filled with coffee capsules made of aluminum and plastic. The sewage from the pre-treatment phase of the wastewater treatment plant of the Federal University of Lavras fed the system. Temperature, pH, alkalinity and volatile acids concentration, COD, TS, TVS and TSS of the influent and effluent were analyzed to evaluate the reactors performances. Statistics tests were run in the software Statistica 10. Changes occurred in the organic loading rates caused two different operating phases,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaerobic filter; Biomass; Immobilization; Support material; Domestic sewage.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100223

Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Bruno,Laura Maria; Lima Filho,José Luiz de; Castro,Heizir Ferreira de.
Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida lipase; Mucor lipase; Hybrid matrix; Immobilization.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003

Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by  Streptomyces lincolnensis Immobilized Cells
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Abdelwahed,Nayera A.M.; Gomaa,Eman Zakaria; Hassan,Amira A.
ABSTRACT Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation. Screening of fermentation medium components to find their relative effect on lincomycin production was done using Plackett-Burman design. Malt extract, dextrin, soluble starch and (NH4)2SO4 were the most significant nutrient influenced on lincomycin production. Central composite design was applied to determine optimal concentrations of these factors and the effect of their mutual interactions. The interaction between soluble starch and (NH4)2SO4 was found to enhance the production, whereas malt extract and dextrin exhibited an influence independent from the other two factors. Using this statistical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Optimization; Lincomycin; Streptomyces lincolnensis; Response surface methodology; Immobilization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100405

The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Vignoli,Josiane Alessandra; Celligoi,Maria Antonia Colabone; Silva,Rui Sérgio Ferreira da; Barros,Márcio de.
The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative effect upon the production of sorbitol, while the time of cultivation and the immobilization process were significant and positive. The results demonstrated that the cell immobilization and the time of cultivation of 36 h presented higher production of sorbitol.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Permeabilization; Immobilization; Sucrose; Z. mobilis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500020

Hydrolysis of whey lactose by immobilized β-Galactosidase
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Mariotti,Marcela Panaro; Yamanaka,Hideko; Araujo,Angela Regina; Trevisan,Henrique Celso.
Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-galactosidase; Immobilization; Lactase; Whey; Lactose; Hydrolysis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019

Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Danial,Enas N.; Hamza,Amal H.; Mahmoud,Rasha H.
This study evaluated the biological importance of immobilized urease enzyme over the free urease. The support material used for urease immobilization was alginate. Generally, the immobilization of urease in alginate gel showed a marked increase in Km and Vmax. However, the immobilized urease showed higher thermal stability than that of free enzyme. The rate of thermal inactivation of the immobilized enzyme decreased due to entrapment in gel matrix. Also, the activity of the immobilized urease was more stable in retention than that of the free enzyme during the storage in solution, although the activity of the immobilized enzyme was lower in comparison with the free enzyme. A stable immobilized system and long storage life are convenient for applications...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urease; Immobilization; Kinetic; Reusability; Storage stability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200147

Histomorphometric analysis of the response of rat skeletal muscle to swimming, immobilization and rehabilitation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nascimento,C.C.F.; Padula,N.; Milani,J.G.P.O.; Shimano,A.C.; Martinez,E.Z.; Mattiello-Sverzut,A.C.
The objective of the present study was to determine to what extent, if any, swimming training applied before immobilization in a cast interferes with the rehabilitation process in rat muscles. Female Wistar rats, mean weight 260.52 ± 16.26 g, were divided into 4 groups of 6 rats each: control, 6 weeks under baseline conditions; trained, swimming training for 6 weeks; trained-immobilized, swimming training for 6 weeks and then immobilized for 1 week; trained-immobilized-rehabilitated, swimming training for 6 weeks, immobilized for 1 week and then remobilized with swimming for 2 weeks. The animals were then sacrificed and the soleus and tibialis anterior muscles were dissected, frozen in liquid nitrogen and processed histochemically (H&amp;E and mATPase)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swimming training; Female rat skeletal muscles; Immobilization; Morphometry; ATPase.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900013

Rat hindlimb joint immobilization with acrylic resin orthoses
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: da Silva,C.A.; Guirro,R.R.J.; Polacow,M.L.O.; Cancelliero,K.M.; Durigan,J.L.Q.
The objective of the present study was to propose an orthosis of light material that would be functional for the animal and that would maintain only the ankle joint immobilized. Male Wistar rats (3 to 4 months old, 250-300 g) were divided into 2 groups (N = 6): control and immobilized for 7 days. Rats were anesthetized with sodium pentobarbital (40 mg/kg weight) and the left hindlimb was immobilized with the orthoses composed of acrylic resin model, abdominal belt and lateral supports. The following analyses were performed: glycogen content of the soleus, extensor digitorum longus, white gastrocnemius, red gastrocnemius, and tibialis anterior muscles by the phenol sulfuric method, and the weight, fiber area and intramuscular connective tissue of the soleus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Hypotrophy; Joint; Orthoses; Physical therapy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700016

Cyclosporin-A does not affect skeletal muscle mass during disuse and recovery
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Aoki,M.S.; Miyabara,E.H.; Soares,A.G.; Salvini,T.F.; Moriscot,A.S.
Cyclosporin-A (CsA) is an immunosuppressive drug that acts as an inhibitor of calcineurin, a calcium phosphatase that has been suggested to play a role in skeletal muscle hypertrophy. The aim of the present study was to determine the effect of CsA administration (25 mg kg-1 day-1) on skeletal muscle mass and phenotype during disuse and recovery. Male Wistar rats received vehicle (N = 8) or CsA (N = 8) during hind limb immobilization (N = 8) and recovery (N = 8). Muscle weight (dry/wet) and cross-sectional area were evaluated to verify the effect of CsA treatment on muscle mass. Muscle phenotype was assessed by histochemistry of myosin ATPase. CsA administration during immobilization and recovery did not change muscle/body weight ratio in the soleus (SOL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcineurin; Immobilization; Recovery; Skeletal muscle; Hypertrophy; Cyclosporin-A.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200011

Limb immobilization alters functional electrophysiological parameters of sciatic nerve
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Alves,J.S.M.; Leal-Cardoso,J.H.; Santos-Júnior,F.F.U.; Carlos,P.S.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Ceccatto,V.M.; Barbosa,R.
Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Sciatic nerve; Compound action potential; Conduction velocity; Rheobase; Chronaxy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715

Analysis of fracture healing in osteopenic bone caused by disuse: experimental study
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Paiva,A.G.; Yanagihara,G.R.; Macedo,A.P.; Ramos,J.; Issa,J.P.M.; Shimano,A.C.
Osteoporosis has become a serious global public health issue. Hence, osteoporotic fracture healing has been investigated in several previous studies because there is still controversy over the effect osteoporosis has on the healing process. The current study aimed to analyze two different periods of bone healing in normal and osteopenic rats. Sixty, 7-week-old female Wistar rats were randomly divided into four groups: unrestricted and immobilized for 2 weeks after osteotomy (OU2), suspended and immobilized for 2 weeks after osteotomy (OS2), unrestricted and immobilized for 6 weeks after osteotomy (OU6), and suspended and immobilized for 6 weeks after osteotomy (OS6). Osteotomy was performed in the middle third of the right tibia 21 days after tail...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Osteotomy; Fracture healing; Immobilization; Rats.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300708

Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gianelo,M.C.S.; Polizzelo,J.C.; Chesca,D.; Mattiello-Sverzut,A.C.
The aim of this study was to determine the effects of intermittent passive manual stretching on various proteins involved in force transmission in skeletal muscle. Female Wistar weanling rats were randomly assigned to 5 groups: 2 control groups containing 21- and 30-day-old rats that received neither immobilization nor stretching, and 3 test groups that received 1) passive stretching over 3 days, 2) immobilization for 7 days and then passive stretching over 3 days, or 3) immobilization for 7 days. Maximal plantar flexion in the right hind limb was imposed, and the stretching protocol of 10 repetitions of 30 s stretches was applied. The soleus muscles were harvested and processed for HE and picrosirius staining; immunohistochemical analysis of collagen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Vimentin; Desmin; Intermittent stretching; Collagen.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200603

Sulfated fucan as support for antibiotic immobilization
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Araújo,P.M.; Oliveira,G.B.; Córdula,C.R.; Leite,E.L.; Carvalho Jr.,L.B.; Silva,M.P.C.
Xylofucoglucuronan from Spatoglossum schröederi algae was tested as a support for antibiotic immobilization. The polysaccharide (20 mg in 6 ml) was first activated using carbodiimide, 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide methiodide (20 mg in 2 ml), under stirring for 1 h at 25ºC and pH from 4.5 to 5.0. After adjusting the pH to 8.0, either gentamicin or amikacin (62.5 mg in 1.25 ml) was then immobilized on this chemically modified polysaccharide with shaking for 24 h in a cold room. Infrared spectra of the activated carbodiimide xylofucoglucuronan showed two bands to carbonyl (C = O at 1647.9 and 1700.7 cm-1) and to amide (C&lt;FONT FACE=Symbol&gt;Ý&lt;/FONT&gt;-NH2) groups (1662.8 and 1714.0 cm-1). Microbial characterization of the derivatives...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sulfated fucan; Immobilization; Antibiotics; Gentamicin; Amikacin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300002

Immobilization and therapeutic passive stretching generate thickening and increase the expression of laminin and dystrophin in skeletal muscle
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cação-Benedini,L.O.; Ribeiro,P.G.; Prado,C.M.; Chesca,D.L.; Mattiello-Sverzut,A.C.
Extracellular matrix and costamere proteins transmit the concentric, isometric, and eccentric forces produced by active muscle contraction. The expression of these proteins after application of passive tension stimuli to muscle remains unknown. This study investigated the expression of laminin and dystrophin in the soleus muscle of rats immobilized with the right ankle in plantar flexion for 10 days and subsequent remobilization, either by isolated free movement in a cage or associated with passive stretching for up to 10 days. The intensity of the macrophage response was also evaluated. One hundred and twenty-eight female Wistar rats were divided into 8 groups: free for 10 days; immobilized for 10 days; immobilized/free for 1, 3, or 10 days; or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Muscle stretching; Soleus muscle; Laminin; Dystrophin; Mechanotransduction.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600483

Effect of one stretch a week applied to the immobilized soleus muscle on rat muscle fiber morphology
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gomes,A.R.S.; Coutinho,E.L.; França,C.N.; Polonio,J.; Salvini,T.F.
We determined the effect of stretching applied once a week to the soleus muscle immobilized in the shortened position on muscle fiber morphology. Twenty-six male Wistar rats weighing 269 ± 26 g were divided into three groups. Group I, the left soleus was immobilized in the shortened position for 3 weeks; group II, the soleus was immobilized in the shortened position and stretched once a week for 3 weeks; group III, the soleus was submitted only to stretching once a week for 3 weeks. The medial part of the soleus muscle was frozen for histology and muscle fiber area evaluation and the lateral part was used for the determination of number and length of serial sarcomeres. Soleus muscle submitted only to immobilization showed a reduction in weight (44 ± 6%, P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle; Stretching; Immobilization; Sarcomere number; Muscle fiber area.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000005

Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Almeida,S.A.; Petenusci,S.O.; Anselmo-Franci,J.A.; Rosa-e-Silva,A.A.M.; Lamano-Carvalho,T.L.
We investigated whether chronic stress applied from prepuberty to full sexual maturity interferes with spermatogenic and androgenic testicular functions. Male Wistar rats (40 days old) were immobilized 6 h a day for 60 days. Following immobilization, plasma concentrations of corticosterone and prolactin increased 135% and 48%, respectively, while plasma luteinizing hormone and testosterone presented a significant decrease of 29% and 37%, respectively. Plasma concentration of follicle-stimulating hormone was not altered in stressed rats. Chronic stress reduced the amount of mature spermatids in the testis by 16% and the spermatozoon concentration in the cauda epididymidis by 32%. A 17% reduction in weight and a 42% decrease in DNA content were observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Spermatogenesis; LH; FSH; Testosterone; Prolactin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100013

The effect of a low dose of clenbuterol on rat soleus muscle submitted to joint immobilization
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cancelliero,K.M.; Durigan,J.L.Q.; Vieira,R.P.; Silva,C.A.; Polacow,M.L.O.
The aim of the present study was to evaluate the effect of joint immobilization on morphometric parameters and glycogen content of soleus muscle treated with clenbuterol. Male Wistar (3-4 months old) rats were divided into 4 groups (N = 6 for each group): control, clenbuterol, immobilized, and immobilized treated with clenbuterol. Immobilization was performed with acrylic resin orthoses and 10 µg/kg body weight clenbuterol was administered subcutaneously for 7 days. The following parameters were measured the next day on soleus muscle: weight, glycogen content, cross-sectional area, and connective tissue content. The clenbuterol group showed an increase in glycogen (81.6%, 0.38 ± 0.09 vs 0.69 ± 0.06 mg/100 g; P &lt; 0.05) without alteration in weight,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Clenbuterol; Connective tissue; Glycogen content; Morphometry; Rat soleus muscle.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200003

"A comparison between sugar consumption and ethanol production in wort by immobilized Saccharomyces Cerevisiae, Saccharomyces Ludwigii and Saccharomyces Rouxii on Brewer's Spent Grain"
Provedor de dados: Brazilian Journal of Microbiology
Autores: Mohammadi,Aniseh; Razavi,Seyyed Hadi; Mousavi,Seyyed Mohammad; Rezaei,Karamatollah.
The immobilization of Saccharomyces cerevisiae DSM 70424, Saccharomyces ludwigii DSM 3447 and Saccharomyces rouxii DSM 2531 on brewer's spent grain and then ethanol production and sugar consumption of these immobilized yeasts were investigated. The aim of this study was to investigate the abilities of these three immobilized yeasts for producing alcohol for brewing at two temperatures (7 and 12 °C) using two different sugar levels (one at original level supplied in the brewery and one with 2.5% (w/v), added glucose to the wort). Increasing both parameters resulted in higher alcohol production by all the yeasts studied. At 7 °C and with original wort density the ethanol content at the end of fermentation was 2.7% (v/v) for S. cerevisiae, 1.7% for S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brewer's spent grain; Fermentation; Immobilization; Saccharomyces cerevisiae; Saccharomyces ludwigii; Saccharomyces rouxii.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200025

Whole cells in enantioselective reduction of benzyl acetoacetate
Provedor de dados: Brazilian Journal of Microbiology
Autores: Ribeiro,Joyce Benzaquem; Ramos,Aline de Souza; Lopes,Raquel de Oliveira; Silva,Gabriela Veloso Vieira da; Souza,Rodrigo Octavio Mendonça Alves de.
The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotransformation; Chiral reduction; Β-ketoester; Kluyveromyces marxianus; Immobilization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300023

Biodegradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1
Provedor de dados: Brazilian Journal of Microbiology
Autores: Tallur,Preeti N.; Mulla,Sikandar I.; Megadi,Veena B.; Talwar,Manjunatha P.; Ninnekar,Harichandra Z.
Pyrethroid pesticide cypermethrin is a environmental pollutant because of its widespread use, toxicity and persistence. Biodegradation of such chemicals by microorganisms may provide an cost-effective method for their detoxification. We have investigated the degradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1 in various matrices such as, polyurethane foam (PUF), polyacrylamide, sodium alginate and agar. The optimum temperature and pH for the degradation of cypermethrin by immobilized cells of Micrococcus sp. were found to be 30 °C and 7.0, respectively. The rate of degradation of 10 and 20 mM of cypermethrin by freely suspended cells were compared with that of immobilized cells in batches and semi-continuous with shaken...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Degradation; Immobilization; Cypermethrin; Polyurethane foam; Micrococcus sp. strain CPN 1.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300667

Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase
Provedor de dados: Brazilian Journal of Microbiology
Autores: Araújo,Maria Elisa Melo Branco de; Campos,Paula Renata Bueno; Alberto,Thiago Grando; Contesini,Fabiano Jares; Carvalho,Patrícia de Oliveira.
Abstract The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w) for Aspergillus niger lipase and 1:5 (w/w) for Rhizopus javanicus lipase using Amberlite MB-1. Both lipases maintained constant activity for 6 months at 4 °C. Reaction time, sardine-free fatty acids:soybean oil mole ratio and initial water content of the lipase were investigated to determine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipase; Immobilization; Soybean oil; Polyunsaturated fatty acids.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000401006

Thiocyanate degradation by pure and mixed cultures of microorganisms
Provedor de dados: Brazilian Journal of Microbiology
Autores: Souza-Fagundes,Elaine M.; Rosa,Luiz H.; Gomes,Newton C.M.; Santos,Mércia H.; Pimentel,Patricia F.
A mixed culture and a pure bacterial strain (BMV8) were isolated from a bioreactor for thiocyanate treatment. Both cultures removed 5 mM of thiocyanate from the medium in 36 hours. The mixed culture was able to tolerate concentrations up to 60 mM. The efficiency of thiocyanate degradation decreased when the cells were immobilized.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradation; Cyanate; Immobilization; Microorganisms; Thiocyanate.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300012

Biodegradation of acetonitrile by cells of Candida guilliermondii UFMG-Y65 immobilized in alginate, kappa-carrageenan and citric pectin
Provedor de dados: Brazilian Journal of Microbiology
Autores: Dias,João Carlos T.; Rezende,Rachel P.; Linardi,Valter R.
Different encapsulation matrices were tested for immobilized cells of Candida guilliermondii UFMG-Y65 used for acetonitrile degradation. Acetonitrile degradation by free cells and cells immobilized in Ba-alginate, kappa-carrageenan and citric pectin was studied. The rate of acetonitrile degradation was monitored for 120 h by measuring yeast growth and ammonia concentration. Different alginate concentrations did not affect cell viability, but the period of incubation in BaCl2 solution reduced the number of viable cells. Likewise, the gel nature and the matrix structure of the support resulting from the cell immobilization conditions were of fundamental importance for biocatalyst activity and performance, affecting substantially the patterns of microbial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida guilliermondii; Acetonitrile; Biodegradation; Immobilization.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100015

Efficiency of decolorization of different dyes using fungal biomass immobilized on different solid supports
Provedor de dados: Brazilian Journal of Microbiology
Autores: Przystaś,Wioletta; Zabłocka-Godlewska,Ewa; Grabińska-Sota,Elżbieta.
Abstract Different technologies may be used for decolorization of wastewater containing dyes. Among them, biological processes are the most promising because they seem to be environmentally safe. The aim of this study was to determine the efficiency of decolorization of two dyes belonging to different classes (azo and triphenylmethane dyes) by immobilized biomass of strains of fungi (Pleurotus ostreatus - BWPH, Gleophyllum odoratum - DCa and Polyporus picipes - RWP17). Different solid supports were tested for biomass immobilization. The best growth of fungal strains was observed on the washer, brush, grid and sawdust supports. Based on the results of dye adsorption, the brush and the washer were selected for further study. These solid supports adsorbed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Decolorization; Fungi; Azo dyes; Triphenylmethane dyes.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200285

Production of Poly-γ-Glutamate (PGA) Biopolymer by Batch and Semicontinuous Cultures of Immobilized Bacilluslicheniformis strain-R
Provedor de dados: Brazilian Journal of Microbiology
Autores: Berekaa,Mahmoud M.; El Aassar,Samy A.; El-Sayed,Samia M.; EL Borai,Aliaa M.
Production of Polyglutamate (PGA) biopolymer by immobilized Bacilluslicheniformis strain-R was intensively investigated. Preliminary experiments were carried out to address the most suitable immobilization methodology. Entrapment of Bacillus cells in alginate-agar led optimal PGA production (36.75 g/l), with 1.32- and 2.18-fold increase in comparison with alginate- or K-carrageenan-immobilized cells, respectively. During semicontinuous cultivation of agar-alginate gel-cell mixture, production of PGA by 10 ml mixture was increased from 2nd to 3rd run whereas, increased till the 4th run using 15ml mixture. Adsorption was the most suitable immobilization technique for production of PGA and the sponge cubes was the preferred matrix recording 43.2 g/l of PGA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Poly-g-glutamate; Bacillu slicheniformis; Immobilization; Bioreactor; Semicontinuous production.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400001

Immobilization of ammonia-oxidizing bacteria by polyvinyl alcohol and sodium alginate
Provedor de dados: Brazilian Journal of Microbiology
Autores: Dong,Yuwei; Zhang,Yanqiu; Tu,Baojun.
Abstract Ammonia-oxidizing bacteria were immobilized by polyvinyl alcohol (PVA) and sodium alginate. The immobilization conditions and ammonia oxidation ability of the immobilized bacteria were investigated. The following immobilization conditions were observed to be optimal: PVA, 12%; sodium alginate, 1.1%; calcium chloride, 1.0%; inoculum concentration, 1.3 immobilized balls/mL of immobilized medium; pH, 10; and temperature, 30 °C. The immobilized ammonia-oxidizing bacteria exhibited strong ammonia oxidation ability even after being recycled four times. The ammonia nitrogen removal rate of the immobilized ammonia-oxidizing bacteria reached 90.30% under the optimal immobilization conditions. When compared with ammonia-oxidizing bacteria immobilized by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammonia-oxidizing bacteria; Immobilization; Polyvinyl alcohol; Sodium alginate; Ammonia oxidation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300515

Stability of free and immobilized Lactobacillus acidophilus and Bifidobacterium lactis in acidified milk and of immobilized B. lactis in yoghurt
Provedor de dados: Brazilian Journal of Microbiology
Autores: Grosso,Carlos Raimundo Ferreira; Fávaro-Trindade,Carmen Sílvia.
This study evaluated the stability of Bifidobacterium lactis (Bb-12) and of Lactobacillus acidophilus (La-05) both free and immobilized in calcium alginate, in milk and in acidified milk (pH 5.0, 4.4 and 3.8). The stability of immobilized B. lactis in yoghurt (fermented to pH 4.2), during 28 days of refrigerated storage was also evaluated. The efficiency of two culture media (modified MRS agar and Reinforced Clostridial Agar plus Prussian Blue) for counting of B. lactis in yoghurt was determined. Lee's agar was used to count Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus when B. lactis were counted in the MRS medium. B. lactis and L. acidophilus in both free and immobilized forms presented satisfactory rates of survival in milk...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotics; Immobilization; Alginate; Lactobacillus acidophilus; Bifidobacterium lactis; Yoghurt; Milk; Lactobacillus bulgaricus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100025

Simultaneous biodegradation of phenol and cyanide present in coke-oven effluent using immobilized Pseudomonas putida and Pseudomonas stutzeri
Provedor de dados: Brazilian Journal of Microbiology
Autores: Singh,Utkarsh; Arora,Naveen Kumar; Sachan,Preeti.
ABSTRACT Discharge of coke-oven wastewater to the environment may cause severe contamination to it and also threaten the flora and fauna, including human beings. Hence before dumping it is necessary to treat this dangerous effluent in order to minimize the damage to the environment. Conventional technologies have inherent drawbacks however, biological treatment is an advantageous alternative method. In the present study, bacteria were isolated from the soil collected from the sites contaminated by coke-oven effluent rich in phenol and cyanide. Nucleotides sequence alignment and phylogenetic analysis showed the identity of the selected phenol and cyanide degrading isolates NAUN-16 and NAUN-1B as Pseudomonas putida and Pseudomonas stutzeri, respectively....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 16S rRNA; Biodegradation; Immobilization; Pseudomonas; Saw dust.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100038

Continuous ethanol production using immobilized yeast cells entrapped in loofa-reinforced alginate carriers
Provedor de dados: Brazilian Journal of Microbiology
Autores: Bangrak,Phoowit; Limtong,Savitree; Phisalaphong,Muenduen.
A culture of Saccharomyces cerevisiae M30 entrapped in loofa-reinforced alginate was used for continuous ethanol fermentation in a packed-bed reactor with initial sugar concentrations of 200-248 g/L. Maximum ethanol productivity of 11.5 g/(L·h) was obtained at an ethanol concentration of 57.4 g/L, an initial sugar concentration of 220 g/L and a dilution rate (D) of 0.2 h-1. However, a maximum ethanol concentration of 82.1 g/L (productivity of 9.0 g/(L·h)) was obtained at a D of 0.11 h-1. Ethanol productivity in the continuous culture was 6-8-fold higher than that in the batch culture. Due to the developed carrier's high biocompatibility, high porosity, and good mechanical strength, advantages such as cell regeneration, reusability, altered mechanical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Loofa; Alginate; Immobilization; Continuous.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200032