Consulta: subjectFacets:"Immunochromatographic test"
Registros recuperados: 3
Data/hora: 13/06/2024 04:38:03

Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa
Provedor de dados: 19
Autores: NGUYEN, Thu-Thuy; MOTSIRI, Mono Sophie; TAIOE, Moeti Oriel; MTSHALI, Moses Sibusiso; GOTO, Yasuyuki; KAWAZU, Shin-Ichiro; Molifi THEKISOE, Oriel Matlhahane; INOUE, Noboru; 河津, 信一郎; 井上, 昇.
A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant...
Palavras-chave: Animal African trypanosomosis; ELISA; Immunochromatographic test; Serodiagnosis; South Africa.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4034

Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
Provedor de dados: 55
Autores: Paulini,Inarei; Siqueira-Silva,Joselma; Thomaz,Luciana; Rocha,Leticia; Harsi,Charlotte; Bellei,Nancy; Granato,Celso.
Abstract Human adenoviruses comprise an important group of etiologic agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adenovirus; Monoclonal antibodies; Immunochromatographic test.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500500

Performance of “RESIST-3 O.K.N. K-SeT” immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey
Provedor de dados: 58
Autores: Sağıroğlu,Pınar; Hasdemir,Ufuk; Gelmez,Gülşen Altınkanat; Aksu,Burak; Karatuna,Onur; Söyletir,Güner.
ABSTRACT In this study, the performance of the “RESIST-3 O.K.N. K-SeT” (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n = 22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for bla KPC, bla IMP, bla VIM, bla NDM, and bla OXA-48. The rates of bla NDM, bla OXA-48, and bla KPC in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both bla NDM and bla OXA-48 were found in six (5.9%) isolates. The results of the assay showed 100% concordance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Immunochromatographic test; NDM; KPC; OXA-48.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400885