Consulta: subjectFacets:"Immunofluorescence"
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Data/hora: 11/06/2024 05:29:31

Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
Provedor de dados: 69
Autores: Pavlov,Maria Soledad; Eissler,Yoanna; Espinoza,Juan Carlos; Garland,David E; Kuznar,Juan.
A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody.
Tipo: Journal article
Palavras-chave: Digital image analysis; Fluorescence microscope methods; Immunofluorescence; Infectious pancreatic necrosis virus (IPNV); SYBR-Green I.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011

Production of polyclonal antisera to manganese superoxide dismutase expressed in downy mildew resistant pearl millet and its application for immunodiagnosis
Provedor de dados: 69
Autores: Urs Babitha,Mysore Pandurangaraj; Madhu,Devaiah; Sripathi P,Harishchandra; Shekar Shetty,Hunthrike.
Level of superoxide dismutase (SOD) in the seedlings of downy mildew resistant pearl millet genotype (IP 18293) increased 3-fold upon inoculation with Sclerospora graminicola. Mn-SOD was purified from pearl millet by ion-exchange and gel filtration chromatography. Polyclonal antibody was raised in rabbit against Mn-SOD of pearl millet. Anti-Mn SOD had an antibody titer of 1:20,000. ELISA results revealed a 1.5-fold increase in pure Mn-SOD preparation compared to the crude preparation. The antibody reactivity as detected by ELISA revealed that Mn-SOD is higher at 4 hrs post-inoculation in resistant pearl millet seedlings. The reactivity also showed the Mn-SOD is more in roots of inoculated resistant seedlings. SOD reactivity was also determined by DIBA...
Tipo: Journal article
Palavras-chave: DIBA; Downy mildew; ELISA; Immunofluorescence; Manganese superoxide dismutase; Pearl millet; Sclerospora graminicola; Western blot.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200007

Noções de sorologia aplicadas à fitovirologia.
Provedor de dados: 14
Autores: ALMEIDA, A. M. R.
Aspectos imunológicos; Anticorpos; Determinantes antigênicos; Produção de antissoros; Métodos sorológicos; Detecção e quantificação de vírus pelo teste de ELISA; Purificação de imunoglobulina; Preparo do conjugado; Preparação de F(ab')2; Soluções tamponantes utilizadas no teste de ELISA; Interpretação dos resultados de ELISA; Anticorpos monoclonais; Anexos: Produção de massa micelial, imunização e produção de antissoro contra fungos; Purificação de antígenos por eletroforese; Produção de células bacterianas, imunização e produção de antissoro; Determinação do teor de proteína pelo método de Bradford; Modelo de ficha para controle de imunização de animais; Modelo de caixa para o manuseio de coelhos durante o processo de sangria; Modelo de ficha para...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Serological methods; Viral plant pathogens; Identification; Imunoelectrophoresis; Immunofluorescence; Sorology; Brasil; Parana; Sorologia; Imunoeletroforese; Imunoflorescencia; Plant; Disease; Fungus.; Bactéria; Elisa; Doença; Fungo; Planta; Vírus.; Brazil; Detection.
Ano: 1995
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/449395

The Na+/K+/2Cl(-) cotransporter in the sea bass Dicentrarchus labrax during ontogeny: involvement in osmoregulation
Provedor de dados: 5
Autores: Lorin, N; Boulo, Viviane; Bodinier, C; Charmantier, G.
This study combines a cellular and molecular analysis of the Na+/K+/2Cl(-) cotransporter (NKCC) to determine the osmoregulatory role of this protein in different tissues during the ontogeny of the sea bass. We have characterized the complete sequence of the NKCC1 isoform isolated from the sea bass gills and have identified, by immunofluorescence, NKCC1, and other isoforms, within the epithelium of the major osmoregulatory organs. Different (absorptive and secretory) functions have been attributed to this protein according to the tissue and salinity. The effects of short- (1-4 days), medium- (7-21 days) and long (6 months)-term freshwater (FW) adaptations were investigated, in comparison with seawater (SW)-maintained sea bass. In adult sea bass after...
Tipo: Text
Palavras-chave: Quantitative expression; Immunofluorescence; Osmoregulation; Ontogeny; Dicentrarchus labrax; Na+/K+/2Cl( ) cotransporter.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-3531.pdf

Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom
Provedor de dados: 80
Autores: Costal-Oliveira,Fernanda; Guerra-Duarte,Clara; Oliveira,Maira Souza; Castro,Karen Larissa Pereira de; Lopes-de-Sousa,Leticia; Lara,Aline; Gomes,Enéas Ricardo de Morais; Bonilla,Cesar; Guatimosim,Sílvia; Melo,Marília Martins; Chávez-Olórtegui,Carlos.
Abstract  Background Hadruroides lunatus is the most abundant scorpion species in the Peruvian central coast, where most of the accidents involving humans are registered. In spite of its prevalence, there are only very few studies on H. lunatus envenomation. The aim of the present study was to analyze the cardiorespiratory alterations caused by H. lunatus envenomation in rodents.  Methods  Wistar rats injected with H. lunatus scorpion venom were submitted to electrocardiography. After euthanasia, rat lungs were collected and histopathologically analyzed. Mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. Data were analyzed by ANOVA or Student’s t-test. The significance level was set at p&lt; 0.05.  Results  It was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hadruroides lunatus venom; Cardiorespiratory alterations; Electrocardiography; Immunofluorescence; Calcium transient.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100301

Diagnostic Methods for the Detection of Cryptosporidium parvum Oocysts in Faeces
Provedor de dados: 19
Autores: Webster, K. A.; Green, J. A.; Dawson, C.; Giles, M.; Catchpole, J.
Palavras-chave: Cryptosporidium parvum; Diagnosis; Immunofluorescence; ELISA; Coproscopical.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/253

Protein and mRNA expression of gonadotropin-releasing hormone receptor in yaks during estrus
Provedor de dados: 96
Autores: Huo,Sheng-dong; Zhang,Tao-jie; Abudureyimu,Ayimuguli; Liu,Jun-lin; Zhang,Guo-hua; Ma,Zhong-ren.
ABSTRACT To demonstrate the role of gonadotropin-releasing hormone (GnRH) in yaks (Bos grunniens), we characterized the expression of gonadotropin-releasing hormone receptor (GnRHR) mRNA and protein. The level of GnRHR mRNA in the hypothalamus was higher than that in the pineal gland, pituitary gland, and ovary during estrus. Immunofluorescence analysis showed that GnRHR was expressed in the pinealocyte, synaptic ribbon, and synaptic spherules of the pineal gland and that melatonin interacts with GnRHR via nerve fibers. In the hypothalamus, GnRHR was expressed in the magnocellular neurons and parvocellular neurons. In the pituitary gland, GnRHR was expressed in acidophilic cells and basophilic cells. In the ovary, GnRHR was present in the ovarian follicle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos grunniens; Gonadotropin-releasing hormone receptor; Immunofluorescence; Real-time PCR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100401

Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)
Provedor de dados: 96
Autores: Huo,Shengdong; Yuan,Qijian; Zhang,Taojie; Liu,Junlin; Abudureyimu,Ayimuguli; Yang,Jutian.
ABSTRACTThe objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens). The analysis showed that the level of expression of ER mRNA was greater in the pituitary gland tissue than in other glands during estrus. Immunofluorescence analyses showed that ER proteins were located in the pineal cells, synaptic ribbon, and synaptic spherules of the pineal gland. In the hypothalamus, ER proteins were located in the magnocellular and parvocellular neurons. The ER proteins were located in acidophilic cells and basophilic cells in the pituitary gland. In the ovary, ER proteins were present in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunofluorescence; Real-time PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350

Comparative study of direct and indirect immunofluorescence for diagnosis of canine pemphigus foliaceus
Provedor de dados: 45
Autores: Severo,J.S.; Aoki,V.; Santana,A.E.; Mantovani,M.M.; Michalany,N.S.; Larsson Junior,C.E.; Larsson,C.E.
ABSTRACT Pemphigus foliaceus (PF) is the most common autoimmune skin disease in dogs. It is characterized by pustules, erosions, and crusts which occur due to the presence of autoantibodies that target intercellular adhesion. Histopathological examination is considered the gold standard pattern in the diagnosis, but may sometimes be inconclusive, especially when the characteristic findings are not identified. New diagnostic tests are continuously being developed and immunofluorescence assays, could be a valuable alternative diagnostic tool. This study aimed to evaluate the applicability of direct and indirect immunofluorescence (DIF and IIF) tests for the diagnosis of canine PF. Twenty eight dogs were divided into two groups: Group I with 14 dogs with PF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunofluorescence; DIF; IIF; Pemphigus foliaceus.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000300649

Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
Provedor de dados: 48
Autores: Soler,Maritza C; Molina,Jessica L; Díaz,Hugo A; Pinto,Vivian C; Barrios,Yasenka L; He,Kan; Roller,Marc; Weinstein-Oppenheimer,Caroline R.
Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine...
Tipo: Journal article
Palavras-chave: Cimicifuga foetida; Cytotoxicity; Hsp-27; Immunofluorescence; MCF-7 cells; RT-PCR.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005

Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients
Provedor de dados: 55
Autores: Carraro,Emerson; Pasternak,Jacyr; Perosa,Ana; Siqueira,Itacy; Martino,Marines Dalla Vale.
Cytomegalovirus (CMV) antigenemia is still one of the two major assays available for diagnosis and monitoring of CMV infections. A commercial rapid test recently available in Brazil for quantification of human cytomegalovirus pp65 antigenemia revealed by immunofluorescence technique was compared with the original in-house method revealed by immunoperoxidase in patients receiving solid organ transplants. Of 80 blood samples tested for CMV antigenemia, 34 (42.5%) were positive: commercial assay detected 33 (97%) and in-house assay detected 20 (58.8%) samples. The numbers of positive cells in the two assays were different, with a median of 4.5 and 12 positive cells obtained by in-house and commercial kit, respectively. Discrepancies between assays occurred in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytomegalovirus; Antigenemia assay; Immmunoperoxidase; Immunofluorescence.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300023

Comparison of immunoperoxidase and immunofluorescence assays for pp65 cytomegalovirus antigen in immunocompromised patients
Provedor de dados: 55
Autores: Debur,Maria do Carmo; Vidal,Luine R. Rosele; Nogueira,Meri Bordignon; Pereira,Luciane Aparecida; Takahashi,Gislene R.A.; Rotta,Indianara; Almeida,Sérgio Monteiro de; Raboni,Sonia Mara.
We compared the pp65 antigen detection by an in house method (immunoperoxidase assay) and by a commercial kit (immunofluorescence assay) available for cytomegalovirus infection diagnosis in immunocompromised patients. Sixty-four blood samples were analyzed in duplicate for both techniques. Eight-six percent of the samples had concordant qualitative results. The discordant results occurred more frequently in samples with low quantity of positive cells. There were no significant differences with qualitative and quantitative results of the methods.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunoperoxidase; Immunofluorescence; Cytomegalovirus; Immunocompromised patients; Bone marrow transplantation; Solid organ transplant.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200014

High seroprevalence of Simkania negevensis in Jordan
Provedor de dados: 58
Autores: Al-Younes,Hesham M.; Paldanius,Mika.
The bacterium Simkania negevensis is a germ associated with respiratory diseases. This study aims at estimating the prevalence of Simkania in the Jordanian population. Serum samples from 664 Jordanian males and females, aged 2 to 86 years were collected. IgG and IgM Simkania-specific antibodies were detected using an indirect immunofluorescence test. Seropositivity titers for IgG and IgM were defined as 1:8 and 1:10, respectively. The overall prevalence of IgG antibody in all examined Jordanian nationals was 58.4%. IgG seropositivity was low in children under the age of 10 years (34.2%), and increased rapidly with age and ranged between 49.4% and 72%. Simkania-specific IgM was detected in 24.8% of subjects. IgM prevalence in children under 10 years was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jordan; Immunofluorescence; Prevalence; Simkania.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400038

Human respiratory syncytial virus detection in children admitted at a community hospital in Botucatu, SP, Brazil
Provedor de dados: 58
Autores: Bosso,Patrícia Alves Ramos; Candeias,João Manuel Grisi; Paduan,Karina dos Santos; Ricchetti,Sandra Mara Queiroz; Miranda,Antero Frederico Macedo de; Rugolo,Lígia Maria Suppo de Souza; Durigon,Edison Luiz; Ventura,Armando Morais.
Human Respiratory Syncytial Virus (HRSV) is recognized as the single most important pathogen responsible for acute viral infection of the lower respiratory tract in young children. In this study, 84 samples from children under two years of age presenting acute respiratory symptoms were obtained from September 2000 to November 2001. Analysis by immunofluorescence and reverse-transcription followed by PCR, revealed that 18% (15/84) of all the samples were positive for HRSV, in which 80% (12/15) of the cases was observed in children under six months of age and the subgroups A and B co-circulated in the studied period. These are the first data obtained for Botucatu city and seasonality is evident by the higher circulation of the virus between May and July.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human Respiratory Syncytial Virus (HRSV); RT-PCR; Immunofluorescence; Seasonality.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300015

DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS IN CALVES OF RIO GRANDE DO SUL, BRAZIL
Provedor de dados: 65
Autores: Gonçalves,Ivan Paulo Demartini; Simanke,Agda Theisen; Jost,Heidy Christine; Hôtzel,Isidro; Soglio,Adriana Dal; Moojen,Valéria.
During 20 months of the 1987-1990 period, lung tissue samples from 351 calves were obtained at a slaughterhouse. These calves were from counties nearby Porto Alegre. The direct and indirect fluorescent antibody tests (FAT) using polyclonal and monoclonal antibody conjugates were performed on frozen lung sections. Eighteen (5.13%) of the calf lung samples were positive for the Bovine Respiratory Syncytial Virus (BRSV). The BRSV was isolated from FAT positive samples.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Bovine respiratory syncytial virus; Immunofluorescence.
Ano: 1993
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000300029