Consulta: subjectFacets:"In vitro propagation"
Registros recuperados: 18
Data/hora: 03/06/2024 19:14:54

Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker
Provedor de dados: 69
Autores: Tiwari,Jagesh Kumar; Saurabh,Shaumaya; Chandel,Poonam; Singh,Bir Pal; Bhardwaj,Vinay.
Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum. Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) were applied to detect the genetic and epigenetic changes, respectively in the somatic hybrids mother plants (1st cycle) and their regenerants (30th cycles sub-cultured). Results: To detect genetic changes, eight AFLP primer combinations yielded a total of 329 scorable bands of which 49 bands were polymorphic in both mother plants and regenerants. None of the scorable bands were observed in term of loss of original band of mother plant or gain of novel band...
Tipo: Journal article
Palavras-chave: AFLP; DNA methylation; In vitro propagation; MSAP; Potato; Somatic hybrids.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600005

Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture
Provedor de dados: 69
Autores: Parida,Reena; Mohanty,Sujata; Kuanar,Ananya; Nayak,Sanghamitra.
An efficient protocol has been established for rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga L, a rare medicinal plant. Different plant growth regulators like Benzyladenine (BA), Indoleacetic acid (IAA), Indolebutyric acid (IBA), Napthaleneacetic acid (NAA) and adenine sulphates (Ads) have been tried for induction of multiple shoots using lateral bud of rhizome as explants. The highest rate of shoot multiplication (11.5 ± 0.6) shoot/explant as well as leaf biomass production (7.4 ± 0.3) gram/explant was observed on Murashige and Skoog medium supplemented with Benzyladenine (1 mg/l) and Indoleacetic acid (0.5 mg/l). Data of shoot multiplication and leaf biomass production were statistically analysed. Upon excission of...
Tipo: Journal article
Palavras-chave: Growth regulators; Kaempferia; In vitro propagation; Leaf biomass.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400005

Selection of reference genes for normalization of quantitative real-time PCR in cell cultures of Cyclamen persicum
Provedor de dados: 69
Autores: Hoenemann,Claudia; Hohe,Annette.
As a prerequisite for gene expression analyses in cell cultures of the ornamental crop Cyclamen persicum basic parameters for quantitative real-time polymerase chain reaction (qRT-PCR) have been established including the selection of reference genes using the software tools &amp;#8216;geNorm&amp;#8217; and &amp;#8216;NormFinder&amp;#8217;. Five potential reference genes have been tested (elongation factor tu (Ef-Tu), putative ABC transporter ATPase, putative conserved oligomeric Golgi (COG) complex component, V-ATPase G subunit 1 and Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4)). &amp;#8216;NormFinder&amp;#8217; as well as &amp;#8216;geNorm&amp;#8217; identified Ef-Tu to be the least stable reference gene while the ranking of the most stable genes...
Tipo: Journal article
Palavras-chave: Gene expression analysis; In vitro propagation; Primer design; Somatic embryogenesis.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100012

Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion
Provedor de dados: 75
Autores: Sivanesan,Iyyakkannu; Kyoung,Kim E; Kyoung,Kim M; Young,Ko E; Park,Se W.
ABSTRACT: The study was undertaken to examine the effect of two synthetic auxins on somatic embryogenesis and plant regeneration from mature embryo explants of 16 onion cultivars. Cotyledons were removed from the embryos and remaining portions were cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 5.0 mg/L picloram alone or in combination (1.0 mg/L 2,4-D + 2.5 mg/L picloram) to produce embryogenic callus. MS medium supplemented with 5.0 mg/L picloram was found to be the best one for both embryogenic callus induction (85%) and callus diameter (3.8 mm). Of the 16 cultivars studied, Yeoeuijuhwang exhibited the lowest frequency of embryogenic callus induction (50.5%), whereas all the other 15 cultivars showed more than 60% embryogenic callus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Auxin; Embryogenic callus; In vitro propagation; Picloram.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362015000400441

Automacao e racionalizacao na micropropagacao industrial.
Provedor de dados: 14
Autores: FARI, M.; MELO, N. F. de.
Tendencias da mecanizacao na propagacao in vitro em larga escala, automacao das etapas dos metodos tradicionais de cultura de tecidos, novastecnologias baseadas na utilizacao de sistemas de propagacao em meio liquido, desenvolvimento de sistemas de cultivo in vitro alternativos, sistema CLONER: uma nova iniciativa de racionalizacao da bancada de micropropagacao.
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Propagacao in vitro; In vitro propagation; Biotecnologia; Cultura de Tecido; Meristema; Micropropagação; Biotechnology; Micropropagation; Tissue culture.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/131977

Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden &amp;amp; Reichb.f. (Orchidaceae)
Provedor de dados: 109
Autores: Salazar-Mercado,Seir Antonio; Vega-Contreras,Nelson Alfonso.
Abstract Cattleya trianae (Linden &amp; Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organic additives; Coconut water; Pineapple juice; In vitro propagation.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000400544

In vitro germination and growth of Vriesea incurvata Gaudich. (Bromeliaceae)
Provedor de dados: 109
Autores: Pulido-Rueda,Edwin Ernesto; Milaneze-Gutierre,Maria Auxiliadora; Negrelle,Raquel.
Abstract Vriesea incurvata in the natural environment shows some difficulties associated to the low seed germination capacity. Aiming to provide basis for the seedlings production, the results of the seeds germination percentage and the initial growth of V. incurvata seedlings in vitro conditions in different culture media, are reported. Completely randomized design was comprised of eight treatments and eight replications. The treatments were as culture media: MS (Murashige &amp; Skoog); MS%; KC (Knudson); KC%; MS + activated carbon (AC); MSV2 + AC; KC + AC; and KCV2 + AC. The germination percentage was calculated from the division between the number of seeds with primary root extrusion by the total number of sowed seeds. The initial growth was evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliaceae; Culture media; In vitro propagation; Native plant; Seed germination.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000100140

Evaluation of the micropropagation potential of curauá pineapple hybrids for fiber production
Provedor de dados: 36
Autores: SOUZA,Cintia Paula Feitosa; SOUZA,Everton Hilo de; LEDO,Carlos Alberto da Silva; SOUZA,Fernanda Vidigal Duarte.
ABSTRACT Plant fiber is a renewable and biodegradable material that can be used effectively to reinforce various composites. Pineapple hybrids selected for their fiber quality are in the phase of agronomic validation in Brazil by the Embrapa Cassava and Fruits research unit. The selection of a hybrid for large-scale fiber production depends on obtaining a large number of seedlings. This study evaluated the morphogenetic response and propagation potential of eight hybrids of Ananas comosus var. erectifolius, for the purpose of producing high-quality seedlings on a large scale. Stem and crown buds were reduced and placed in MS nutritive medium supplemented with BAP at 0.5 mg L-1, NAA at 0.01 mg L-1 and Phytagel® at 2.5 g L-1. After 45 days, the number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus (L.) Merr.; In vitro propagation; Lignocellulosic fibers; Poisson log-linear model; Geometric growth rates.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672018000400290

Abiotic environmental conditions for germination and development of gametophytes of Cyathea phalerata Mart. (Cyatheaceae)
Provedor de dados: 39
Autores: Marcon,Catiuscia; Silveira,Tatieli; Schmitt,Jairo Lizandro; Droste,Annette.
ABSTRACT In order to successfully establish themselves in their natural environment, ferns need habitats with abiotic conditions that are suitable for spore germination and gametophyte development. The objective of this study was to assess the influence of abiotic factors on the initial development of Cyathea phalerata cultivated in vitro. Spore germination and gametophyte development were assessed under varying conditions of surface sterilization, pH, temperature and photoperiod. Exogenous contamination was eliminated by sterilizing spores with 2.5 % NaClO for 15 min and sowing them into a culture medium supplemented with nystatin. Spores germinated at all pHs tested. Gametophytic development was faster in acidic pHs. Cultures at 25 °C exhibited the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic factors; Arborescent ferns; In vitro propagation; Reproduction; Spores.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100058

Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
Provedor de dados: 3
Autores: Gomes, Lucas Roberto Pereira; Franceschi, Cristina do Rosário Batista; Ribas, Luciana Lopes Fortes.
An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotecnologia; Botânica epiphytic orchid; In vitro propagation; Protocorms; Protocorm-like body 2.03.00.00-0.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276

Micropropagation of Glandularia perakii Cov. et Schn. (Verbenaceae): a native species with ornamental potential
Provedor de dados: 47
Autores: Marino,Concepción; Ponce,María T.; Videla,María E.; Fioretti,Sonia; Cirrincione,Miguel.
Glandularia perakii is a perennial species with beautiful violet flowers that grows in the stony soil of Mendocine pedemont. A plentiful and prolonged flowering confers it an important ornamental potential. In this paper, a method of propagation of G. perakii from nodal segments is reported. Proliferating microshoot cultures were obtained by placing nodal segment on Murashige and Skoog medium (MS) supplemented with 20 g.L-1 of sucrose without growth regulators. In this medium multiplication rate after 20 days was 7.9. Rooted plants were acclimatized successfully.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tissue culture; In vitro propagation; Nodal segment; Glandularia perakii.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100007

Pigment production and growth of Alternanthera plants cultured in vitro in the presence of tyrosine
Provedor de dados: 52
Autores: Kleinowski,Alítcia Moraes; Brandão,Isabel Rodrigues; Einhardt,Andersom Milech; Ribeiro,Márcia Vaz; Peters,José Antonio; Braga,Eugenia Jacira Bolacel.
The aim of the present study was to investigate the influence of tyrosine on the in vitro growth and the production of the betacyanin pigment in Alternanthera philoxeroides and A. tenella. Nodal segments were inoculated in MS medium containing different concentrations of tyrosine (0, 25, 50 and 75 μM), and the number of sprouts and buds, height, root length, fresh matter of shoots and roots and betacyanin content were evaluated. In A. philoxeroides , the highest production of betacyanin (51.30 mg 100 g-1 FM) was in the stems with the addition of approximately 45 μM tyrosine, while the increase in the leaves was proportional to the tyrosine concentration, and the best average was obtained with a tyrosine concentration of 75 μM (15.32 mg 100 g-1 FM). Higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Betacyanin; Elicitors; In vitro propagation; Alligator weed; Joy weed.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200014

In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots
Provedor de dados: 117
Autores: García-Gonzáles,Rolando; Delgado,Miladys; González,Yailín; González,Aníbal; Garriga,Miguel; Caligari,Peter  D.S; Carrasco,Basilio; Quiroz,Karla.
Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to develop new plantations and assist in establishing improvement programs for this species. The aim of this study was to develop a protocol to establish in vitro conditions and to micropropagate this species from nodal explants from juvenile cuttings taken from field trees. Disinfection of node explants with 5% propiconazole CE 25 during 3 min resulted in 100% explant disinfection and 60% morphogenic response on those established explants. Shoot development was optimized by cultivating in vitro node explants in Murashige and Skoog basal medium...
Tipo: Journal article
Palavras-chave: Organogenesis; In vitro propagation; In vitro rooting; Plant growth regulators.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300005

In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile
Provedor de dados: 118
Autores: Paredes,Kelly; Delaveau,Catherine; Carrasco,Pedro; Baeza,Carlos; Mora,Freddy; Uribe,Matilde E.
K. Paredes, C. Delaveau, P. Carrasco, C. Baeza, Freddy Mora, and M.E. Uribe. 2014. In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile. Cien. Inv. Agr. 41(2): 207-214. Critically endangered, Traubia modesta is endemic to Chile and belongs to the family Amaryllidaceae. In this research, a propagation protocol was developed for the in vitro cultivation of vegetative organs for this species. The twin scale explants were cultured in a Murashige and Skoog (MS) medium, supplemented with the growth regulators naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) in different concentrations. Plant preservative mixture (PPM) and polyvinyl pyrrolidone (PVP) were used as a biocide and an antioxidant,...
Tipo: Journal article
Palavras-chave: Bulbing; Critically endangered species; In vitro propagation; Traubia modesta; Twin scales.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000200007

Micropropagation of Chapada Diamantina ornamental bromeliad
Provedor de dados: 65
Autores: Lima,Andressa Priscila Piancó Santos; Brito,Alone Lima; Santana,José Raniere Ferreira de.
ABSTRACT: Sincoraea mucugensis (Wand. &amp; A.A. Conc.) LOUZADA &amp; WAND, an endangered bromeliad, is confined to the central region of the Chapada Diamantina, in the municipality of Mucugê, Brazil. From various researches, it is evident that for the propagation of this species, the in vitro technique is a feasible option. However, due to the low multiplication rates reported in various papers, this study aimed to establish a micropropagation protocol of direct organogenesis for S. mucugensis. First, the inoculation of the stem explants was done in MS ½ culture medium which contained different levels of BAP (0.00; 6.66; 8.88; 11.10; 13.20 µM) and NAA (0.00; 2.60; 5.20 µM). These shoots were then subjected to a couple of distinct rooting periods (of 30-...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro propagation; Acclimatization; Sincoraea mucugensis; NAA.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200400

Microshoots encapsulation and plant conversion of Musa sp. cv. 'Grand Naine'
Provedor de dados: 65
Autores: Sandoval-Yugar,Edgar Wilfredo; Dal Vesco,Lírio Luiz; Steinmacher,Douglas André; Stolf,Elaine Cristina; Guerra,Miguel Pedro.
Synthetic seed technologies are useful tools for the field delivery of in vitro derived plantlets. In the present study, different encapsulation procedures and their efficacy in the plantlet regeneration using microshoots of banana cv. 'Grand Naine' were evaluated. Two encapsulation systems were evaluated: i) single encapsulation in beads or droplet hardening method; and ii) double layer or hollow beads. The use of different compounds to enhance the capsule conservation and the conversion to plantlets was also evaluated. The conversion capacity was assessed in vitro on water-agar culture medium or in ex vitro conditions with Gerbox® boxes. The single encapsulation system showed 80% conversion. The capsules with MS saline formulation treated with 100mM KNO3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artificial seeds; Banana; In vitro propagation; Pharmaceutical capsules.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000400007

In vitro germination and acclimatization of cambui tree type seedlings
Provedor de dados: 65
Autores: Lédo,Ana da Silva; Barin,Luciana Borin; Silva,Ana Veruska Cruz da; Sá,Francielen Paola de; Machado,Caroline de Araújo.
There are few reports in literature on the in vitro behavior of cambui tree (Myrciaria tenella O. Berg) and acclimatization conditions. The aim of this study was to evaluate the effect of culture media on in vitro germination and the effect of different substrates on the acclimatization of two Myrciaria tenella types. The study was carried out at the Embrapa Tabuleiros Costeiros Laboratory of Plant Tissue Culture, Aracaju, SE. Seeds were extracted from fruits of two Myrciaria tenella types: Orange and Purple Types. The seeds were inoculated in the following culture media: T1 - MS medium + 30g L -1  sucrose, T2 - 1/2 MS medium + 15g L -1 sucrose and T3 - control without MS salts. To study the effect of substrates on acclimatization, seedlings were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrciaria tenella O. Berg; In vitro propagation; Substrates; Fruticulture.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801355

In vitro development of Encyclea cordigera in different concentrations of honey
Provedor de dados: 65
Autores: Mantovani,Cibele; Pivetta,Kathia Fernandes Lopes.
ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige &amp; Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian orchid; In vitro propagation; Carbohydrate source.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400590