Consulta: subjectFacets:"Integrin"
Registros recuperados: 13
Data/hora: 09/06/2024 05:27:15

Integrins, cancer and snake toxins (mini-review)
Provedor de dados: 80
Autores: Rádis-Baptista,G.
Integrins encompass a family of transmembrane heterodimeric proteins of adhesion that maintain cells attached to other cells and to the extracellular matrix (ECM). Integrins work as bi-directional mechanotransducers, conveying mechanical signal from outside to inside the cell through a cascade of phosphorylation signals. On the other hand, the signal from inside to outside controls the strength and affinity of integrin adhesion. As proteins of focal contact, integrins are involved in diverse cell functions, such as cell activation, migration, growth, and survival. In the development of neoplastic disease and metastatic tumor, integrins can influence cancer invasiveness and progression, as well as mediate the formation of new blood vessels (angiogenesis)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Integrin; Angiogenesis; Cancer; Apoptosis; Snake toxin; Snake venom C-type lectin; Metalloprotease; Disintegrin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300002

Alternagin-C binding to α2β1 integrin controls matrix metalloprotease-9 and matrix metalloprotease-2 in breast tumor cells and endothelial cells
Provedor de dados: 80
Autores: Moritz,Milene Nóbrega de Oliveira; Eustáquio,Lívia Mara Santos; Micocci,Kelli Cristina; Nunes,Ana Carolina Caetano; Santos,Patty Karina dos; Vieira,Tamires de Castro; Selistre-de-Araujo,Heloísa Sobreiro.
Abstract  Background Matrix metalloproteinases (MMPs) are key players in tumor progression, helping tumor cells to modify their microenvironment, which allows cell migration to secondary sites. The role of integrins, adhesion receptors that connect cells to the extracellular matrix, in MMP expression and activity has been previously suggested. However, the mechanisms by which integrins control MMP expression are not completely understood. Particularly, the role of α2β1 integrin, one of the major collagen I receptors, in MMP activity and expression has not been studied. Alternagin-C (ALT-C), a glutamate-cysteine-aspartate-disintegrin from Bothrops alternatus venom, has high affinity for an α2β1 integrin. Herein, we used ALT-C as a α2β1 integrin ligand to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ALT-C; Α2β1; Integrin; Cancer; Tumor microenvironment; MMP; C-Myc.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100307

Integrin-linked kinase is involved in lactoferrin-induced anchorage-independent cell growth and survival in PC12 cells
Provedor de dados: 19
Autores: Ishii, Toshiaki; Uto, Tomoka; Mori, Kaori; Fujikawa, Ryu; 石井, 利明.
Aims: Bovine lactoferrin (bLf) causes anchorage-independent cell growth in PC12 cells. The present study investigated the mechanisms involved in bLf-induced anchorage-independent cell growth and survival in PC12 cells. Main methods: The number of adherent cells and suspended cells was estimated separately by using a methyl thiazol tetrazolium (MTT) assay, and the sum of both optical density (O.D.) (570 nm) values was used as a measure of the total number of cells. Key findings: Integrin-linked kinase (ILK) plays an important role in integrin and growth factor signaling pathways. Stable transfection of PC12 cells with a dominant negative kinase-deficient mutant of ILK (DN-ILK) inhibited bLf-induced anchorage-independent cell growth. The ILK activity in the...
Palavras-chave: Bovine lactoferrin; PC12 cell; Integrin-linked kinase; Integrin; P38 MAPK; Cell suspension; Cell adhesion; Cell growth; Cell viability; Dominant-negative mutant; Anchorage-independent growth.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2675

Glycosynapses: microdomains controlling carbohydrate-dependent cell adhesion and signaling
Provedor de dados: 42
Autores: Hakomori,Senitiroh.
The concept of microdomains in plasma membranes was developed over two decades, following observation of polarity of membrane based on clustering of specific membrane components. Microdomains involved in carbohydrate-dependent cell adhesion with concurrent signal transduction that affect cellular phenotype are termed "glycosynapse". Three types of glycosynapse have been distinguished: "type 1" having glycosphingolipid associated with signal transducers (small G-proteins, cSrc, Src family kinases) and proteolipids; "type 2" having O-linked mucin-type glycoprotein associated with Src family kinases; and "type 3" having N-linked integrin receptor complexed with tetraspanin and ganglioside. Different cell types are characterized by presence of specific types...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clustering; Carbohydrate-to-carbohydrate interaction; Carbohydrate-binding protein; Integrin; Tetraspanin; Growth factor receptor.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000300010

Integrin-&amp;#946;1, not integrin-&amp;#946;5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
Provedor de dados: 48
Autores: Zeng,Qiangcheng; Guo,Yong; Liu,Yongming; Li,Ruixin; Zhang,Xinchang; Liu,Lu; Wang,Yang; Zhang,Xizheng; Zou,Xianqiong.
BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-&amp;#946; (&amp;#946;1, &amp;#946;5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-&amp;#946; in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-&amp;#946;1 siRNA or integrin-&amp;#946;5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µ&amp;#949;) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted...
Tipo: Journal article
Palavras-chave: Tensile strain; Osteoblast; Extracellular matrix; Integrin; SiRNA.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025

Differential expression of integrin subunits on adherent and nonadherent mast cells
Provedor de dados: 56
Autores: Grodzki,A.C.G.; Dávila Pástor,M.V.; Sousa,J.F.; Oliver,C.; Jamur,M.C.
Mast cell progenitors arise in bone marrow and then migrate to peripheral tissues where they mature. It is presumed that integrin receptors are involved in their migration and homing. In the present study, the expression of various integrin subunits was investigated in three systems of adherent and nonadherent mast cells. Mesentery mast cells, freshly isolated bone marrow-derived mast cells (BMMC) and RBL-2H3 cells grown attached to tissue culture flasks are all adherent mast cells and peritoneal mast cells, and cultured BMMC and RBL-2H3 cells grown in suspension represent nonadherent mast cell populations. Pure populations of mast cells were immunomagnetically isolated from bone marrow, mesentery and peritoneal lavage using the mast cell-specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mast cells; Integrin; Mouse.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800017

Dimensions and dynamics in integrin function
Provedor de dados: 56
Autores: Yamada,K.M.; Pankov,R.; Cukierman,E.
Integrins play crucial roles in cell adhesion, migration, and signaling by providing transmembrane links between the extracellular matrix and the cytoskeleton. Integrins cluster in macromolecular complexes to generate cell-matrix adhesions such as focal adhesions. In this mini-review, we compare certain integrin-based biological responses and signaling during cell interactions with standard 2D cell culture versus 3D matrices. Besides responding to the composition of the matrix, cells sense and react to physical properties that include three-dimensionality and rigidity. In routine cell culture, fibroblasts and mesenchymal cells appear to use focal adhesions as anchors. They then use intracellular actomyosin contractility and dynamic, directional integrin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Cell adhesion; Integrin; Fibronectin; Signal transduction; Migration.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800001

Simultaneous changes in the function and expression of beta 1 integrins during the growth arrest of poorly differentiated colorectal cells (LISP-1)
Provedor de dados: 56
Autores: Roela,R.A.; Brentani,M.M.; Katayama,M.L.H.; Reis,M.; Federico,M.H.H.
Cells usually lose adhesion and increase proliferation and migration during malignant transformation. Here, we studied how proliferation can affect the other two characteristics, which ultimately lead to invasion and metastasis. We determined the expression of ß1 integrins, as well as adhesion and migration towards laminin-1, fibronectin, collagens type I and type IV presented by LISP-1 colorectal cancer cells exposed to 2.5% dimethyl sulfoxide (DMSO), an agent capable of decreasing proliferation in this poorly differentiated colorectal cell line. Untreated cells (control), as shown by flow cytometry and monoclonal antibodies, expressed alpha2 (63.8 ± 11.3% positive cells), alpha3 (93.3 ± 7.0%), alpha5 (50.4 ± 12.0%) and alpha6 (34.1 ± 4.9%) integrins but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell adhesion molecules; Cell migration; Integrin; Cell cycle; DMSO.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800016

Snake venom metalloproteinases and disintegrins: interactions with cells
Provedor de dados: 56
Autores: Kamiguti,A.S.; Zuzel,M.; Theakston,R.D.G.
Metalloproteinases and disintegrins are important components of most viperid and crotalid venoms. Large metalloproteinases referred to as MDC enzymes are composed of an N-terminal Metalloproteinase domain, a Disintegrin-like domain and a Cys-rich C-terminus. In contrast, disintegrins are small non-enzymatic RGD-containing cysteine-rich polypeptides. However, the disintegrin region of MDC enzymes bears a high degree of structural homology to that of the disintegrins, although it lacks the RGD motif. Despite these differences, both components share the property of being able to recognize integrin cell surface receptors and thereby to inhibit integrin-dependent cell reactions. Recently, several membrane-bound MDC enzymes, closely related to soluble venom MDC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom; Metalloproteinase; Disintegrin; Platelet; Integrin; Signaling.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700001

A role for angiogenesis in rheumatoid arthritis
Provedor de dados: 56
Autores: Stupack,D.G.; Storgard,C.M.; Cheresh,D.A.
Rheumatoid arthritis (RA) is a chronic debilitating disease characterized by distinct autoimmune, inflammatory and fibrovascular components which lead to synovial proliferation and joint destruction. However, existing treatments specifically target only autoimmune and inflammatory components despite the fact that neovascularization of the inflamed synovium is a hallmark of rheumatoid arthritis. Angiogenesis may contribute to synovial growth, leukocyte recruitment and tissue remodeling, thus potentiating disease progression. Although no therapies currently target angiogenesis, several existing therapies have anti-angiogenic activity. Recent advances in anti-angiogenic strategies in oncology, including the identification of integrin &lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiogenesis; Endothelium; Arthritis; Integrin.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500011

Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation
Provedor de dados: 56
Autores: Mermelstein,C.S.; Rebello,M.I.L.; Amaral,L.M.; Costa,M.L.
Although much is known about the molecules involved in extracellular Ca2+ regulation, the relationship of the ion with overall cell morphology is not understood. The objective of the present study was to determine the effect of the Ca2+ chelator EGTA on the major cytoskeleton components, at integrin-containing adhesion sites, and their consequences on cell shape. Control mouse cell line C2C12 has a well-spread morphology with long stress fibers running in many different directions, as detected by fluorescence microscopy using rhodamine-phalloidin. In contrast, cells treated with EGTA (1.75 mM in culture medium) for 24 h became bipolar and showed less stress fibers running in one major direction. The adhesion plaque protein alpha5-integrin was detected by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytoskeleton; Extracellular Ca2+; Cell shape; Microfilaments; Microtubules; Integrin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800018

Disintegrins: integrin selective ligands which activate integrin-coupled signaling and modulate leukocyte functions
Provedor de dados: 56
Autores: Barja-Fidalgo,C.; Coelho,A.L.J.; Saldanha-Gama,R.; Helal-Neto,E.; Mariano-Oliveira,A.; Freitas,M.S. de.
Extracellular matrix proteins and cell adhesion receptors (integrins) play essential roles in the regulation of cell adhesion and migration. Interactions of integrins with the extracellular matrix proteins lead to phosphorylation of several intracellular proteins such as focal adhesion kinase, activating different signaling pathways responsible for the regulation of a variety of cell functions, including cytoskeleton mobilization. Once leukocytes are guided to sites of infection, inflammation, or antigen presentation, integrins can participate in the initiation, maintenance, or termination of the immune and inflammatory responses. The modulation of neutrophil activation through integrin-mediated pathways is important in the homeostatic control of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Integrin; Disintegrin; Leukocytes; Activation; Proliferation; Signal transduction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000008

Leukocyte adhesion - a fundamental process in leukocyte physiology
Provedor de dados: 56
Autores: Gahmberg,C.G.; Valmu,L.; Tian,L.; Kotovuori,P.; Fagerholm,S.; Kotovuori,A.; Kantor,C.; Hilden,T.
Leukocyte adhesion is of pivotal functional importance. The adhesion involves several different adhesion molecules, the most important of which are the leukocyte ß2-integrins (CD11/CD18), the intercellular adhesion molecules, and the selectins. We and others have extensively studied the specificity and binding sites in the integrins and the intercellular adhesion molecules for their receptors and ligands. The integrins have to become activated to exert their functions but the possible mechanisms of activation remain poorly understood. Importantly, a few novel intercellular adhesion molecules have been recently described, which seem to function only in specific tissues. Furthermore, it is becoming increasingly apparent that changes in integrins and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukocyte; Adhesion; Integrin; ICAM; Membrane; Glycoprotein.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500003