Consulta: subjectFacets:"Interferon-induced protein 44"
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Data/hora: 05/06/2024 04:27:50

Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1
Provedor de dados: 5
Autores: Renault, Tristan; Faury, Nicole; Barbosa Solomieu, Valérie; Moreau, Kevin.
Virus-induced genes were identified using suppression subtractive hybridisation (SSH) from Pacific cupped oyster, Crassostrea gigas, haemocytes challenged by OsHV-1. A total of 304 clones from SSH forward library were sequenced. Among these sequences, some homologues corresponded to (i) immune related genes (macrophage express protein, IK cytokine, interferon-induced protein 44 or multicopper oxidase), (ii) apoptosis related genes (Bcl-2) and (iii) cell signalling and virus receptor genes (glypican). Molecular characterization and phylogenic analysis of 3 immune-related genes (macrophage expressed protein, multicopper oxidase and immunoglobulin domain cell adhesion molecule) were performed. Finally, quantitative PCR revealed significant changes in the...
Tipo: Text
Palavras-chave: Pacific cupped oyster; Crassostrea gigas; OsHV-1; Immunity; Gene expression; Multicopper oxidase; Macrophage expressed protein; Ig cell adhesion molecule; Interferon-induced protein 44; Virus RNA.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00043/15466/12843.pdf