Consulta: subjectFacets:"Internode"
Registros recuperados: 3
Data/hora: 03/06/2024 17:58:58

Isolation of total RNA from hard bamboo tissue rich in polyphenols and polysaccharides for gene expression studies
Provedor de dados: 69
Autores: Rai,Vineeta; Ghosh,Jayadri Sekhar; Dey,Nrisingha.
RNA isolation from hard and woody internodal bamboo (Bambusa balcooa) tissue is very difficult due to the presence of secondary metabolites, polysaccharides, and polyphenolics. These compounds often co-precipitate with isolated RNA and hinder downstream applications. We have developed an efficient, cost effective and reproducible RNA isolation method from hard tissue of bamboo internode. This protocol includes an additional organic solvent refinement steps to remove endogenous phenolic compounds and acidic phenol (pH 4.2) to critically stabilize RNA in extraction buffer. In addition to these, two 2M Lithium chloride washing steps were introduced to eliminate DNA and polysaccharides contamination. The RNA isolated from the present protocol was found to be...
Tipo: Journal article
Palavras-chave: Acid phenol; Bambusa balcooa; Fiber specific genes; Internode; RNA extraction.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500022

Morphological development of Alfagraze cultivar and Crioula alfalfa populations selected at the seedling stage
Provedor de dados: 96
Autores: Favero,Daniela; Scheffer-Basso,Simone Meredith; Carneiro,Cerci Maria.
The objective of this study was to compare two populations of Crioula alfalfa cultivars, selected by the length of the seedling second internode (Crioula-LI, long internode = 2.5 cm; Crioula-SI, short internode = 1.5 cm) with Alfagraze cultivar in order to verify the seedling morphology value for the selection of more prostrate plants. The plants were grown individually in pots in a semi-protected environment and harvested at 74, 104, 134, 164, 194 and 224 days. Crioula populations varied in terms of height, leaf area, root volume, dry matter yield, and the Crioula-LI population outnumbered that of Crioula-SI. For height, length of internode and primary stem, Alfagraze (45.1 cm, 2.1 cm and 16.7 cm) was similar to Crioula-SI (37.6 cm, 2.3 cm and 18.2 cm);...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding; Grazing-type; Hay-type; Internode.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000400003

Involvement of peroxidases in structural changes of barley stem
Provedor de dados: 53
Autores: Lidija,Begović; Lepeduš,Hrvoje; Lalić,Alojzije; Štolfa,Ivna; Jurković,Zorica; Kovačević,Josip; Cesar,Vera.
ABSTRACT The aim of this study was to investigate anatomical characteristics of barley (Hordeum vulgare L.) internodes and to reveal the connection with activity of guaiacol-peroxidases, their isoenzyme profiles, lignin, and cellulose content. Three lower internodes were sampled at elongation stage and differed in their anatomical features by showing that lignin deposition follows developmental pattern. Epidermis and hypodermis in the first internode were characterized by continuous layer of the lignified cells while in the second and third internode thin walled parenchyma cells could be observed in the hypodermis. These observations were in accordance with increment of peroxidase activity from the first to the third internode. Two acidic and 2 basic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lignin; Cell wall; Internode; Hordeum vulgare L.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000300352