Consulta: subjectFacets:"Isochrysis"
Registros recuperados: 4
Data/hora: 16/06/2024 17:05:28

Effects of blue light on the biochemical composition and photosynthetic activity of Isochrysis sp. (T-iso)
Provedor de dados: 5
Autores: Marchetti, Julie; Bougaran, Gael; Jauffrais, Thierry; Lefebvre, Sebastien; Rouxel, Catherine; Saint-jean, Bruno; Lukomska, Ewa; Robert, Rene; Cadoret, Jean-paul.
In aquaculture, particularly in bivalve hatcheries, the biochemical composition of algal diets has a strong influence on larval and post-larval development. Biochemical composition is known to be related to culture conditions, among which light represents a major source of variation. The effects of blue light on biochemical composition and photosynthetic rate of Isochrysis sp. (T-iso) CCAP 927/14 were assessed in chemostat at a single irradiance (300 μmol photons m−2 s−1) and compared with white light. Two different dilution (renewal) rates were also tested: 0.7 and 0.2 d−1. Relative carbohydrate content was lower under blue light than under white light at both dilution rates, whereas chlorophyll a and photosynthesis activity were higher. In contrast,...
Tipo: Text
Palavras-chave: Isochrysis; Blue light; Photosynthesis; Proximate composition; Chemostat.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00085/19599/17237.pdf

Use of a lipid rich strain reveals mechanisms of nitrogen limitation and carbon partitioning in the haptophyte Tisochrysis lutea
Provedor de dados: 5
Autores: Garnier, Matthieu; Bougaran, Gael; Pavlovic, Marija; Berard, Jean-baptiste; Carrier, Gregory; Charrier, Aurelie; Le Grand, Fabienne; Lukomska, Ewa; Rouxel, Catherine; Schreiber, Nathalie; Cadoret, Jean-paul; Rogniaux, Helene; Saint-jean, Bruno.
Haptophytes are a diverse monophyletic group with a worldwide distribution, known to be significantly involved in global climate regulation in their role as a carbon sink. Because nitrogen is a major limiting macronutrient for phytoplankton in oceans and for cultures of microalgae, understanding the involvement of nitrogen availability in haptophyte carbon partitioning is of global and biotechnological importance. Here, we made an ecophysiological study coupled with comprehensive large scale proteomic analysis to examine differences of behavior in reaction to nitrogen availability changes between a wild type strain of Tisochrysis lutea (WTc1) and a mutant strain (2Xc1) known to accumulate more storage lipids. Strains were grown in chemostats and studied...
Tipo: Text
Palavras-chave: Algae; Isochrysis; Lipids; Nitrogen; Reverse genomic: proteomic.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00356/46702/46904.pdf

Transient initial phase in continuous culture of Isochrysis galbana affinis Tahiti
Provedor de dados: 5
Autores: Bougaran, Gael; Le Dean, Loic; Lukomska, Ewa; Kaas, Raymond; Baron, Regis.
Starting microalgae continuous culture generally includes a preliminary batch culture to obtain sufficient cell density. It is possible to apply continuous regime from the beginning of the culture (IC mode) rather than to begin by an initial batch (IB mode). It is our purpose to check that both initial modes lead to identical steady-state cell characteristics. The microalga Isochrysis galbana affinis Tahiti was used for this comparative study. With an initial cell density of 5 x 10(5) cell ml(-1) and a dilution rate of about 1.0 d(-1), both IB and IC modes led to identical cell density once steady-state is reached between 6 and 8 d after inoculation in the two cases. Cell concentration of chlorophyll a and pheopigment a were found to be similar for IB and...
Tipo: Text
Palavras-chave: Isochrysis; Cell quota; Photosynthesis; Steady state; Continuous culture.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-597.pdf

Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure
Provedor de dados: 5
Autores: Bougaran, Gael; Rouxel, Catherine; Dubois, Nolwenn; Kaas, Raymond; Grouas, Sophie; Lukomska, Ewa; Le Coz, Jean-rene; Cadoret, Jean-paul.
Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...)
Tipo: Text
Palavras-chave: Strain selection; Isochrysis; Neutral lipid; Ultra violet; Flux cytometry; Biofuel.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00085/19643/17418.pdf