Consulta: subjectFacets:"Kaurenoic acid"
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Data/hora: 10/06/2024 15:18:44

Kaurene diterpene induces apoptosis in U87 human malignant glioblastoma cells by suppression of anti-apoptotic signals and activation of cysteine proteases
Provedor de dados: 56
Autores: Lizarte Neto,F.S.; Tirapelli,D.P.C.; Ambrosio,S.R.; Tirapelli,C.R.; Oliveira,F.M.; Novais,P.C.; Peria,F.M.; Oliveira,H.F.; Carlotti Junior,C.G.; Tirapelli,L.F.
Gliomas are the most common and malignant primary brain tumors in humans. Studies have shown that classes of kaurene diterpene have anti-tumor activity related to their ability to induce apoptosis. We investigated the response of the human glioblastoma cell line U87 to treatment with ent-kaur-16-en-19-oic acid (kaurenoic acid, KA). We analyzed cell survival and the induction of apoptosis using flow cytometry and annexin V staining. Additionally, the expression of anti-apoptotic (c-FLIP and miR-21) and apoptotic (Fas, caspase-3 and caspase-8) genes was analyzed by relative quantification (real-time PCR) of mRNA levels in U87 cells that were either untreated or treated with KA (30, 50, or 70 µM) for 24, 48, and 72 h. U87 cells treated with KA demonstrated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kaurenoic acid; Glioblastoma; MiR-21; C-FLIP; Apoptosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100071