Consulta: subjectFacets:"Kinetic parameters"
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Batch culture growth of Chlorella zofingiensis on effluent derived from two-stage anaerobic digestion of two-phase olive mill solid waste
Provedor de dados: Electronic Journal of Biotechnology
Autores: Travieso Córdoba,Lissette; Domínguez Bocanegra,Alma Rosa; Rincón Llorente,Bárbara; Sánchez Hernández,Enrique; Benítez Echegoyen,Francisco; Borja,Rafael; Raposo Bejines,Francisco; Colmenarejo Morcillo,Manuel Francisco.
This paper presents the use of an effluent derived from two-stage anaerobic digestion of two-phase olive mill solid waste (OMSW) as a substrate for the production of Chlorella zofingiensis in batch mode. Chlorella zofingiensis when grown autotrophycally can accumulate significant quantities of valuable carotenoids which are used as an additive in fish and poultry farming, as colorants in foods and in health care products. It was found that two-phase OMSW previously treated by two-stage anaerobic digestion and further sterilized may be used as a culture medium for the microalgae Chlorella zofingiensis. Typical growth curves were obtained using both the above-mentioned anaerobic effluent and a synthetic medium. Total chemical oxygen demand (TCOD) and soluble...
Tipo: Journal article
Palavras-chave: Batch culture; Chlorella zofingiensis; Kinetic parameters; Substrate; Two-phase olive mill solid waste; Two-stage anaerobic digestion.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200003

Exoproteolytic activity in an Atlantic pond (France): estimates of in situ activity
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Crottereau, C; Delmas, Daniel.
In order to estimate in situ bacterial exoproteolysis and its contribution to bacterial secondary production (BSP), we studied microbial activities (BSP and kinetics of exoproteolytic activity) in relation to dissolved combined amino acids (DCAA) during the survey of an Atlantic marine pond. In this pond, high concentrations of DCAA (mean = 4.27 mu M), probably generated by zooplankton grazing, allowed large bacterial biomass development and high production (max = 10(11) cells l(-1) and 18.8 x 10(9) cells l(-1) d(-1) respectively). Exoproteolytic enzyme activity (mean V-m = 55.9 mu M d(-1)) was tightly cou pled to bacterial production, suggesting that protein utilization was essential for bacterial growth. Calculation, according to the Michaelis-Menten...
Tipo: Text
Palavras-chave: Bacterioplankton; In situ exoproteolytic hydrolysis rate; Kinetic parameters; DCAA; Marine pond.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/00446/55803/62288.pdf

Comparison in waterborne Cu, Ni and Pb bioaccumulation kinetics between different gammarid species and populations: Natural variability and influence of metal exposure history
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Urien, N.; Farfarana, A.; Uher, E.; Fechner, Lise; Chaumot, A.; Geffard, O.; Lebrun, J. D.
Kinetic parameters (uptake from solution and elimination rate constants) of Cu, Ni and Pb bioaccumulation were determined from two Gammarus pulex and three Gammarus fossrum wild populations collected from reference sites throughout France in order to assess the inter-species and the natural inter-population variability of metal bioaccumulation kinetics in that sentinel organism. For that, each population was independently exposed for seven days to either 2.5 mu g L-1 Cu (39.3 nM), 40 mu g L-1 Ni (681 nM) or 10 mu g L-1 Pb (48.3 nM) in laboratory controlled conditions, and then placed in unexposed microcosms for a 7-day depuration period. In the same way, the possible influence of metal exposure history on subsequent metal bioaccumulation kinetics was...
Tipo: Text
Palavras-chave: Amphipods; Trace metals; Life history; Kinetic parameters; Inter-population variability.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00417/52840/54270.pdf

KINETICS OF SOIL UREASE AFFECTED BY UREASE INHIBITORS AT CONTRASTING MOISTURE REGIMES
Provedor de dados: Revista de la Ciencia del Suelo y Nutrición Vegetal
Autores: Juan,Y.H; Chen,L.J; Wu,Z.J; Wang,R.
With black soil (Pachic Udic Argiboroll) of Northeastern China as the test object, an incubation test was conducted to investigate the effects of urease inhibitors, hydroquinone (HQ), phenyl phosphorodiamidate (PPD) and N-(n-Butyl) thiophosphoric triamide (NBPT), on the kinetic characteristics of soil urease under normal moisture and waterlogged conditions, aimed to study the changes of catalytic potential of soil urease and the inhibition mechanisms. The results showed that test urease exhibited typical Michaelis-Menten kinetic behaviors, and all test inhibitors increased the Km and decreased the Vmax of soil urease, behaving as mixed inhibitors to soil urease. Under both normal and waterlogged conditions, compared with HQ, PPD and NBPT made Km increase...
Tipo: Journal article
Palavras-chave: Kinetic parameters; Mixed inhibition; Moisture regime; Soil urease.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912009000200004

Screening of an adapted culture medium composed by different carbon sources for heterotrophic cultivation of Chlorella vulgaris using a microplate assay
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Fonseca, Gustavo Graciano; Silva, Alisson Alves da.
  The ability of microalgae to grow heterotrophically lies in the ability to oxidize organic compounds present in the external environment. The aim of this work was to evaluate the growth of Chlorella vulgaris under heterotrophic culture conditions using Basal Bold and NPK media supplemented with different sole carbon sources (glucose, fructose, sucrose, glycerol or acetate). The kinetic parameters obtained were maximum specific growth rate (µmax), doubling time (DT), maximal absorbance, which was also converted to cell concentration values using a linear relation, and cell productivity (PX). Among all the treatments analyzed, the highest maximum specific growth rate found was 0.030 hour-1 (0.72 day-1) in the treatment using Basal Bold medium supplemented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.12.00.00-9; 2.12.01.00-5 microalga; Physiology; Kinetic parameters; Growth rate. Biotecnologia.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/39401

Optimized acid hydrolysis of the polysaccharides from the seaweed Solieria filiformis (Kützing) P.W. Gabrielson for bioethanol production
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Castro, George Meredite Cunha de; Benevides, Norma Maria Barros; Cabral, Maulori Curié; Miranda, Rafael de Souza; Gomes Filho, Enéas; Rocha, Maria Valderez Ponte; Araújo, Marjory Lima Holanda.
 The seaweeds are bio-resource rich in sulfated and neutral polysaccharides. The tropical seaweed species used in this study (Solieria filiformis), after dried, shows 65.8% (w/w) carbohydrate, 9.6% (w/w) protein, 1.7% (w/w) lipid, 7.0% (w/w) moisture and 15.9% (w/w) ash. The dried seaweed was easily hydrolyzed under mild conditions (0.5 M sulfuric acid, 20 min.), generating fermentable monosaccharides with a maximum hydrolysis efficiency of 63.21%. Galactose and glucose present in the hydrolyzed were simultaneously fermented by Saccharomyces cerevisiae when the yeast was acclimated to galactose and cultivated in broth containing only galactose. The kinetic parameters of the fermentation of the seaweed hydrolyzed were Y(P⁄S) = 0.48 ± 0.02 g.g−1, PP = 0.27 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ciências da Vida  Bioquímica iota-carrageenan; Cellulose; Galactose; Kinetic parameters; Fermentation; Acclimation; Sulfuric acid. Biotecnologia.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37227

Molecular and functional characterization of ferredoxin NADP(H) oxidoreductase from Gracilaria chilensis and its complex with ferredoxin
Provedor de dados: Biological Research
Autores: Vorphal,María Alejandra; Bruna,Carola; Wandersleben,Traudy; Dagnino-Leone,Jorge; Lobos-González,Francisco; Uribe,Elena; Martínez-Oyanedel,José; Bunster,Marta.
Abstract  Backgroud Ferredoxin NADP(H) oxidoreductases (EC 1.18.1.2) (FNR) are flavoenzymes present in photosynthetic organisms; they are relevant for the production of reduced donors to redox reactions, i.e. in photosynthesis, the reduction of NADP+ to NADPH using the electrons provided by Ferredoxin (Fd), a small FeS soluble protein acceptor of electrons from PSI in chloroplasts. In rhodophyta no information about this system has been reported, this work is a contribution to the molecular and functional characterization of FNR from Gracilaria chilensis, also providing a structural analysis of the complex FNR/Fd.  Methods The biochemical and kinetic characterization of FNR was performed from the enzyme purified from phycobilisomes enriched fractions. The...
Tipo: Journal article
Palavras-chave: Ferredoxin NADP+ reductase; Ferredoxin; Sequence; Kinetic parameters; Structural features.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100228

Determination of kinetic parameters of a lab-scale upflow anaerobic sludge blanket reator (uasb) removing organic loading from swine manure effluents
Provedor de dados: Ciência e Agrotecnologia
Autores: Campos,Cláudio Milton Montenegro; Saléh,Bruno Botelho; Carmo,Fernanda Ribeiro do.
The present work aimed at determining and evaluating the kinetic parameters from the UASB reactor treating swine manure effluent in a lab-scale experiment. The research was carried out in the Laboratory of Water Analysis at the Engineering Department (LAADEG) at the campus of Federal University of Lavras - UFLA. The system was assembled with an acidification and equalization tank (AET), an UASB reactor and an aerated facultative pond (AFP). The hydraulic retention time (HRT) adopted in the UASB reactor were: 55; 39; 34; 24; 17; and 16 hours. The operational average temperature in the UASB reactor was 25 ± 2ºC. The kinetic studies used the following parameters: Chemical Oxygen Demand (COD T), Total Volatile Solids (TVS), Temperature, Flowrate and Total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine effluents; UASB reactor; Kinetic parameters.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000500020

Removal of copper in cachaças using clays
Provedor de dados: Ciência e Agrotecnologia
Autores: Duarte,Felipe Cimino; Cardoso,Maria das Graças; Magriotis,Zuy Maria; Santiago,Wilder Douglas; Mendonça,João Guilherme Pereira; Rodrigues,Leonardo Milani Avelar.
Increased production and cachaça consumption has stimulated research regarding its quality. It is known that copper can be harmful to human health when it is in concentrated form in the beverage and is ingested in large amounts. Thus, there is a great concern among researchers and producers about the use of adsorbent substances for the removal of excess copper. The kinetic parameters and the adsorption isotherms for copper on kaolinite were studied to evaluate the physical-chemical quality of the cachaça before and after adsorption. K01, K03 and K04 kaolinites presented a maximum adsorption capacity of 97, 95 and 90%, respectively. The kinetic rates followed the pseudo-first-order model, and the adsorption isotherm was best adjusted to the Freundlich...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beverage; Kinetic parameters; Isotherms; Adsorption.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000400009