Consulta: subjectFacets:"Kinins"
Registros recuperados: 6
Data/hora: 10/06/2024 04:36:45

Scorpion (Buthus tamulus) venom toxicity on cardiopulmonary reflexes involves kinins via 5-HT3 receptor subtypes
Provedor de dados: 79
Autores: BAGCHI,S.; DESHPANDE,S. B.
The mechanisms underlying the action of Indian red scorpion Buthus tamulus (BT) venom-induced augmentation of cardiopulmonary reflexes elicited by intravenous injection of 5-HT were examined in urethane anaesthetized rats. The 5-HT produced a concentration-dependent increase in time-response area of bradycardiac response, with the responses at submaximal concentrations shifted to the left after exposure to BT venom (20 µg/kg, IV). Aprotinin (6000 kallikrein inactivating unit, IV) as such had no effect on 5-HT reflex responses (bradycardia, hypotension, and apnea), but blocked the venom-induced reflex augmentation. While ondansetron (10 µg/kg, IV) completely blocked the 5-HT reflex responses, these reappeared partially after venom exposure (20 µg/kg)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bezold-Jarisch reflex; Aprotinin; Bradykinin; Indian red scorpion; Ondansetron; Buthus tamulus; Kinins; 5-HT3 receptors; Cardiopulmonary reflexes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000100003

T-kininogen inhibits kinin-mediated activation of ERK in endothelial cells
Provedor de dados: 48
Autores: LEIVA-SALCEDO,ELIAS; PEREZ,VIVIANA; ACUÑA-CASTILLO,CLAUDIO; WALTER,ROBIN; SIERRA,FELIPE.
Serum levels of T-kininogen increase dramatically as rats approach the end of their lifespan. Stable expression of the protein in Balb/c 3T3 fibroblasts leads to a dramatic inhibition of cell proliferation, as well as inhibition of the ERK signaling pathway. T-kininogen is a potent inhibitor of cysteine proteinases, and we have described that the inhibition of ERK activity occurs, at least in part, via stabilization of the MAP kinase phosphatase, MKP-1. Since fibroblasts are not a physiological target of T-kininogen, we have now purified the protein from rat serum, and used it to assess the effect of T-kininogen on endothelial cells. Adding purified T-kininogen to EAhy 926 hybridoma cells resulted in inhibition of basal ERK activity levels, as estimated...
Tipo: Journal article
Palavras-chave: Aging; Endothelial cells; ERK pathway; Kininogen; Kinins.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200020

High expression of human carboxypeptidase M in Pichia pastoris: Purification and partial characterization
Provedor de dados: 56
Autores: Craveiro,R.B.; Ramalho,J.D.; Chagas,J.R.; Wang,P.H.M.; Casarini,D.E.; Pesquero,J.L.; Araújo,R.C.; Pesquero,J.B.
Carboxypeptidase M (CPM) is an extracellular glycosylphosphatidyl-inositol-anchored membrane glycoprotein, which removes the C-terminal basic residues, lysine and arginine, from peptides and proteins at neutral pH. CPM plays an important role in the control of peptide hormones and growth factor activity on the cell surface. The present study was carried out to clone and express human CPM in the yeast Pichia pastoris in order to evaluate the importance of this enzyme in physiological and pathological processes. The cDNA for the enzyme was amplified from total placental RNA by RT-PCR and cloned in the vector pPIC9, which uses the methanol oxidase promoter and drives the expression of high levels of heterologous proteins in P. pastoris. The cpm gene, after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kinins; Human carboxypeptidase M; Recombinant protein; Pichia pastoris.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200007

Molecular biology of the kallikrein-kinin system: from structure to function
Provedor de dados: 56
Autores: Pesquero,J.B.; Bader,M.
The participation of the kallikrein-kinin system, comprising the serine proteases kallikreins, the protein substrates kininogens and the effective peptides kinins, in some pathological processes like hypertension and cardiovascular diseases is still a matter of controversy. The use of different experimental set-ups in concert with the development of potent and specific inhibitors and antagonists for the system has highlighted its importance but the results still lack conclusivity. Over the last few years, transgenic and gene-targeting technologies associated with molecular biology tools have provided specific information about the elusive role of the kallikrein-kinin system in the control of blood pressure and electrolyte homeostasis. cDNA and genomic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kallikrein; Kinins; Bradykinin; Molecular biology; Transgenics.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900013

Bi-directional actions of estrogen on the renin-angiotensin system
Provedor de dados: 56
Autores: Brosnihan,K.B.; Senanayake,P.S.; Li,P.; Ferrario,C.M.
Estrogen stimulates the renin-angiotensin system by augmenting both tissue and circulating levels of angiotensinogen and renin. We show, however, that angiotensin converting enzyme (ACE) activity in the circulation and in tissues is reduced in two animal models of postmenopausal chronic hormone replacement. We observed a reduction of ACE activity in association with a significant increase in plasma angiotensin I (Ang I) and hyperreninemia in ovariectomized monkeys treated with Premarin (conjugated equine estrogen) replacement for 30 months. Plasma angiotensin II (Ang II) levels were not increased in monkeys treated with estrogen, suggesting that the decrease in ACE curtailed the formation of the peptide. The Ang II/Ang I ratio, an in vivo index of ACE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin; Angiotensin converting enzyme; Angiotensin peptides; Angiotensin receptors; Bradykinin; Nitric oxide; Kinins; Vasodilation; Hormone replacement.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400001

Evidence for the presence of a kininogen-like species in a case of total deficiency of low and high molecular weight kininogens
Provedor de dados: 56
Autores: Veloso,D.
Low and high molecular weight kininogens (LK and HK), containing 409 and 626 amino acids with masses of ~65 and 120 kDa after glycosylation, respectively, are coded by a single gene mapped to the human chromosome 3 by alternative splicing of the transcribed mRNA. The NH2-termini Glu1-Thr383 region, identical in LK and HK, contains bradykinin (BK) moieties Arg363-Arg371. LK, HK and their kinin products Lys-BK and BK are involved in several biologic processes. They are evolutionarily conserved and only 7 patients, all apparently normal, have been reported to lack them. In one of these patients (Williams' trait), a codon mutation (Arg178 &lt;FONT FACE="Symbol"&gt;®&lt;/FONT&gt; stop) has been blamed for the absence of LK and HK. However, using Western blots...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kininogen deficiency; Kinins; Antibodies; Plasma; Kininogen-like species; Human and nonhuman primates.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700004