Consulta: subjectFacets:"Klebsiella pneumoniae"
Registros recuperados: 27
Data/hora: 01/06/2024 18:26:12

The dispersal of nitrogen-fixing Enterobacteriaceae from sewage into the waters and sediments of Morecambe Bay, UK.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Jones, K; Betaieb, M.
The sediments of Morecambe Bay contain large numbers of salinity-tolerant, nitrogen-fixing Enterobacteriaceae (NFEs) mainly Klebsiella pneumoniae ) which appear to be derived from sewage. Sewage effluents, regardless as to whether they have received primary or secondary treatment, contain huge numbers of NFEs with salinity-tolerant and freshwater strains present in roughly equal quantities. The bacteria are discharged into the rivers and estuaries and carried out to sea. Once in the sea the salinity-tolerant strains retain their viability better than the freshwater strains so that the incoming tide, which doses the sediments, contains mainly salinity-tolerant NFEs. It is probable that similar processes occur all round the U.K. coast, as inshore coastal...
Tipo: Text
Palavras-chave: Klebsiella pneumoniae; Enterobacteriaceae; Salinity tolerance; Microbial contamination; Pathogenic bacteria; Bacteria; Sewage; Nitrogen fixation; Sediment pollution; Sea water.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1003.pdf

Assessment of genetic relationship between Klebsiella pneumoniae and Klebsiella oxytoca samples isolated from a dental office
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Pimenta-Rodrigues,MV; Fusco-Almeida,AM; Bertoni,BW; Pietro,RCLR.
The present study aimed to analyze the genetic similarity between genomic profiles of thirteen Klebsiella oxytoca and seven Klebsiella pneumoniae samples isolated from two different collections carried out in different places of dental offices. Random amplified polymorphic DNA (RAPD) technique and similarity coefficients (calculated by Sorensen-Dice and simple matching) were applied to determine their genetic profile of randomic DNA sequences. The majority of the isolates of K. pneumoniae and K. oxytoca presented similar coefficient values (e" 0.80). Thus, it was possible to identify that strain dissemination occurred mainly via the hands of the surgeon-dentists and, finally, to determine the genetic similarity of the strains from dental office environments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella oxytoca; Klebsiella pneumoniae; RAPD; Dental office.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400012

Análisis poblacional de células vegetativas de Bacillus thuringiensis en interacción in vitro con bacterias del suelo
Provedor de dados: Agrociencia
Autores: Rojas-Ruiz,Norma E.; Vázquez-Cruz,Candelario; Sánchez-Alonso,Patricia; Sansinenea-Royano,Estibaliz.
La bacteria Bacillus thuringiensis se usa como bioinsecticida agrícola, pero se conoce poco sobre su persistencia en el suelo. El objetivo de este trabajo fue analizar la interacción de las células vegetativas o no esporuladas de B. thuringiensis, con 11 microorganismos nativos de suelo. Las bacterias fueron confrontadas por cocultivo de pares en condiciones controladas, y se evaluaron cinéticas de crecimiento por conteo celular en placa de los microorganismos estudiados. Los resultados mostraron que el desarrollo de B. thuringiensis es afectado por siete de los 11 microorganismos de suelo evaluados. De estos antagonistas, Pseudomonas fluorescens fue uno de los que redujeron más la población de células vegetativas de B. thuringiensis, a 28 °C versus 37 °C;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antagonismo microbiano; Pseudomonas fluorescens; Klebsiella pneumoniae.
Ano: 2010
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000800007

Peracute bovine mastitis caused by Klebsiella pneumoniae
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Ribeiro,M.G.; Motta,R.G.; Paes,A.C.; Allendorf,S.D.; Salerno,T.; Siqueira,A.K.; Fernandes,M.C.; Lara,G.H.B.
Relata-se a ocorrência de graves sintomas de mastite hiperaguda em vaca, causada por Klebsiella pneumoniae, na terceira semana de lactação. Descrevem-se aspectos epidemiológicos, sintomas clínicos, procedimentos de diagnóstico microbiológico, conduta terapêutica e medidas de controle.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Milk; Klebsiella pneumoniae; Endotoxin; Lipopolysaccharide.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200031

Perfil de resistência antimicrobiana de Klebsiella pneumoniae isoladas de animais domésticos e silvestres
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Sousa,A.T.H.I.; Makino,H.; Bruno,V.C.M.; Candido,S.L.; Nogueira,B.S.; Menezes,I.G.; Nakazato,L.; Dutra,V.
RESUMO Klebsiella pneumoniae é um patógeno oportunista, responsável por diversos tipos de infecções nosocomiais, e é considerado um microrganismo multirresistente. Dados na literatura que forneçam informações a respeito da resistência desse microrganismo a antimicrobianos em amostras de animais são escassos. Dessa forma, o objetivo deste trabalho foi avaliar o perfil e o seu aumento das resistências a antimicrobianos dentro da medicina veterinária. Um total de 67 isolados de K. pneumoniae, provenientes de diferentes sítios de isolamento de animais domésticos (39/67) e silvestres (28/67), foi confirmado por sequenciamento do gene 16S rRNA. O maior percentual de isolamento de K. pneumoniae foi de amostras de urina, com 16% (11/67), fezes, com 15% (10/67), e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Multirresistência; Perfil de resistência; MRD.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200584

An outbreak of acute bovine mastitis caused by Klebsiella pneumoniae in a dairy herd
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Silva,N.; Costa,G.M.
An outbreak of coliform mastitis is described in a dairy herd from the State of Rio de Janeiro, Brazil. During a four-month period 14 fatal cases of Klebsiella pneumoniae-related mastitis were observed in a herd of 104 lactating cows. The symptoms included peracute enterotoxemia in which the cows died 6 to 12 h after the detection of mastitis by CMT. Staphylococcus aureus and Streptococcus agalactiae Streptococcus agalactiae were also isolated although could not be associated with cases of acute fatal mastitis. Milking practices were also evaluated. The milking machine was being used correctly and adequate precautions for hygiene and pre-milking and post-milking teat dipping were used. The organism was sensitive to gentamicin. Therapy for acute toxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cow; Acute bovine mastitis; Outbreak; Klebsiella pneumoniae; Vaccination; Treatment.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000400001

Risk factors for KPC-producing Klebsiella pneumoniae bacteremia
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Tuon,Felipe F.; Rocha,Jaime L.; Toledo,Paula; Arend,Lavinia N.; Dias,Camila H.; Leite,Talita M.; Penteado-Filho,Sergio R.; Pilonetto,Marcelo; Zavascki,Alexandre P.
The molecular epidemiology of carbapenemase-producing Klebsiella pneumoniae (KPC) has been largely investigated, but limited clinical information is available. A case-control study was performed to evaluate the risk factors for KPC bacteremia in hospitalized patients. Cases were patients with KPC bacteremia and controls were patients with non-KPC bacteremia. A total of 85 patients were included, 18 (21.2%) were KPC, and 67 (78.8%) were non-KPC (40 [59.7%] of them were extended-spectrum beta-lactamase producers). All KPC isolates were type 2 producers. These isolates belong to five distinct clones. Multivariate analysis showed that age (odds ratio [OR], 1.06; 95% confidence interval [CI], 1.02 - 1.11; p = 0.004), presence of mechanical ventilation (OR,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Bacteremia risk factors; Klebsiella pneumoniae; Carbapenemases; Carbapenems.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500002

Klebsiella pneumoniae with multiple antimicrobial resistance
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Mendes,Caio; Kiffer,Carlos; Segura,Adília; Ribeiro,Julival; Turner,Philip.
A Klebsiella pneumoniae strain was isolated from the urine of a patient at one of the centers participating in the 2001 edition of the MYSTIC program in Brazil. The initial phenotypic findings of the isolated K. pneumoniae presented an unusual MIC of 8 mug/mL to meropenem, 2 mug/mL to imipenem, elevated MICs to broad spectrum cephalosporins (ceftazidime/cefotaxime/cefepime MIC &gt; 256 mug/mL), aminoglycosides (gentamycin &gt; 256 mug/mL and tobramycin = 48 mug/mL), piperacillin/tazobactam (MIC &gt; 256 mug/mL) and susceptibility to ciprofloxacin (MIC = 0.25 mug/mL). The strain also tested positive for ESBL production with double-disk and E-test methodologies. More detailed investigation revealed that the strain produced a SHV-4 type enzyme and also lacked...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Klebsiella pneumoniae; Drug effects; Drug resistance; Carbapenems.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100008

Risk factors for infection by extended-spectrum beta-lactamase producing Klebsiella pneumoniae in a tertiary hospital in Salvador, Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Silva,Nanci; Oliveira,Márcio; Bandeira,Antonio Carlos; Brites,Carlos.
Nosocomial infection caused by extended-spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-Kp) have been frequently reported worldwide. We have no information on such problems in Bahia, Brazil. OBJECTIVES: Evaluate the risk factors for nosocomial infections caused by ESBL-Kp, in a tertiary hospital, in Bahia, Brazil. MATERIAL AND METHODS: We evaluated all reported cases of nosocomial infections caused by ESBL-Kp in a private, tertiary hospital, in Salvador, Brazil, from 2000 through 2004. We compared patients with a diagnosis of ESBL-Kp (cases) and patients infected by non-ESBL producing K. pneumoniae (controls). Mean age, underlying disease, and frequency of invasive procedures were compared between the two groups. History of previous use of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nosocomial infections; ESBL; Klebsiella pneumoniae; Bacterial resistance.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000300007

Characterization of the genetic environment of the bla KPC-2 gene among Klebsiella pneumoniae isolates from a Chinese Hospital
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Shen,Pinghua; Zhang,Ying; Li,Gang; Jiang,Xiaofei.
Abstract Infection caused by carbapenem-resistant Klebsiella pneumoniae has become a major healthcare threat and KPC-2 enzyme is a dominant factor mediating carbapenems resistance in K. pneumoniae. This study was designed to determine the genetic environment of bla KPC-2, which prevailed in clinical K. pneumoniae isolates recovered in Huashan Hospital, Shanghai, China. Forty-two clinical isolates were included in this study by bla KPC-2 screening. After multilocus sequence typing and plasmid analyses of PCR-based replicon typing (PBRT), junction PCR, mapping PCR and crossing PCR assays, primer walking, and amplicon sequencing were used to analyze the genetic environment of the bla KPC-2 gene. ST423, ST65, ST977, and ST11 were all detected in...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Klebsiella pneumoniae; BlaKPC; Genetic environment.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400384

Fatal case of donor-derived colistin-resistant carbapenemase-producing Klebsiella pneumoniae transmission in cardiac transplantation
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Galvão,Lisia Miglioli; Oliveira,Anna Paula Romero de; Ibanês,Aline Santos; Monteiro,Jussimara; Inoue,Fernanda; Dantas,Daniel Chagas; Sanchez,Flavio; Santos,Daniel Wagner; Abboud,Cely Saad.
ABSTRACT Herein we report a fatal case of donor-derived transmission of XDR-resistant carbapenemase-producing Klebsiella pneumoniae (KPC-Kp) in cardiac transplantation. A 59-year-old male patient with non-obstructive hypertrophic cardiomyopathy underwent heart transplantation. On day 5 post-operation, blood cultures from the donor were positive for colistin-resistant carbapenemase-producing K. pneumoniae (ColR KPC-Kp) susceptible only to amikacin. Recipient blood cultures were also positive for ColR KPC-Kp with the same sensitivity profile as the donor isolate with an identical PFGE pattern. The patient was treated with double-carbapenems and amikacin. The patient evolved to pericarditis, osteomyelitis, and pulmonary necrosis, all fragment cultures...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Cardiac transplantation; Klebsiella pneumoniae; Donor-derived infection; Carbapenem-resistant.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300235

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Chagas,Thiago Pavoni Gomes; Alves,Ronaldo Mendes; Vallim,Deyse Christina; Seki,Liliane Miyuki; Campos,Leila Carvalho; Asensi,Marise Dutra.
OBJECTIVE: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. MATERIAL AND METHODS: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC50 and MIC90 for ESBL-producing isolates were determined by the Etest® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for blaTEM, blaSHV, blaCTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Β-lactamases; Genotype.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500002

Klebsiella ESBL bacteremia-mortality and risk factors
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Tuon,Felipe Francisco; Kruger,Margot; Terreri,Marcos; Penteado-Filho,Sergio R; Gortz,Lucas.
Background: Extended spectrum β-lactamase (ESBL)-producing bacteria have become recognized as a problem in South America. The aim of this study was to evaluate risk factors and mortality rate in bacteremia caused by ESBL-producing Klebsiella pneumoniae in a Brazilian hospital. Methods: A three-year retrospective cohort study with 104 cases of K. pneumoniae bacteremia (61 ESBL and 43 non-ESBL). Several clinical and laboratory variables were evaluated. The outcome of interest was 30-day mortality. The adequate treatment was evaluated according to antibiotic susceptibility. Results: Multivariable analysis showed that central venous catheter and mechanical ventilation were independent risk factors for ESBL. The duration of hospitalization before the bacteremia...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Klebsiella pneumoniae; Beta-lactamases; Bacteremia; Carbapenems.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600016

Differential activity of a lectin from Solieria filiformis against human pathogenic bacteria
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Holanda,M.L.; Melo,V.M.M.; Silva,L.M.C.M.; Amorim,R.C.N.; Pereira,M.G.; Benevides,N.M.B.
A lectin isolated from the red alga Solieria filiformis was evaluated for its effect on the growth of 8 gram-negative and 3 gram-positive bacteria cultivated in liquid medium (three independent experiments/bacterium). The lectin (500 µg/mL) stimulated the growth of the gram-positive species Bacillus cereus and inhibited the growth of the gram-negative species Serratia marcescens, Salmonella typhi, Klebsiella pneumoniae, Enterobacter aerogenes, Proteus sp, and Pseudomonas aeruginosa at 1000 µg/mL but the lectin (10-1000 µg/mL) had no effect on the growth of the gram-positive bacteria Staphylococcus aureus and B. subtilis, or on the gram-negative bacteria Escherichia coli and Salmonella typhimurium. The purified lectin significantly reduced the cell density...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Red alga; Solieria filiformis; Human pathogenic bacteria; Lectin; Antibacterial activity; Klebsiella pneumoniae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200005

Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Li,Chao; Fu,Gongyu; Shi,Yaoqiang; Zhang,A-Mei; Xia,Xueshan; Fang,Yue; Mao,Xiaoqin; Jiang,Jie; Song,Yuzhu; Yang,Guangying.
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loop-mediated isothermal amplification; Polymerase chain reaction; Klebsiella pneumoniae; Novel specific gene; Specific; Sensitive method.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300609

Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA
Provedor de dados: Brazilian Journal of Microbiology
Autores: Ashayeri-Panah,Mitra; Eftekhar,Fereshteh; Ghamsari,Maryam Mobarak; Parvin,Mahmood; Feizabadi,Mohammad Mehdi.
In this study, the discriminatory power of pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were compared. All isolates were typeable by RAPD, while 3.6% of them were not typeable by PFGE. The repeatability of both typing methods were 100% with satisfying reproducibility (≥ 95%). Although the discriminatory power of PFGE was greater than RAPD, both methods showed sufficient discriminatory power (DI &gt; 0.95) which reflects the heterogeneity among the K. pneumoniae isolates. An optimized RAPD protocol is less technically demanding and time consuming that makes it a reliable typing method and competitive with PFGE.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Pulsed field gel electrophoresis; Random amplified polymorphic DNA; Genotyping.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300024

Performance of “RESIST-3 O.K.N. K-SeT” immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey
Provedor de dados: Brazilian Journal of Microbiology
Autores: Sağıroğlu,Pınar; Hasdemir,Ufuk; Gelmez,Gülşen Altınkanat; Aksu,Burak; Karatuna,Onur; Söyletir,Güner.
ABSTRACT In this study, the performance of the “RESIST-3 O.K.N. K-SeT” (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n = 22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for bla KPC, bla IMP, bla VIM, bla NDM, and bla OXA-48. The rates of bla NDM, bla OXA-48, and bla KPC in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both bla NDM and bla OXA-48 were found in six (5.9%) isolates. The results of the assay showed 100% concordance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Immunochromatographic test; NDM; KPC; OXA-48.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400885

Atypical phenotypic characteristics of klebsiella pneumoniae isolates from an outbreak in a neonatal intensive care unit in Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Otman,J.; Perugini,M.E.; Tognim,M.C.B.; Vidotto,M.C.
Extended-spectrum beta-Lactamase-producing (ESBL) Klebsiella sp.isolates from an outbreak in a Neonatal Intensive Care Unit (NICU) at a teaching hospital in Londrina, Paraná State, Brazil, presented atypical phenotypic characteristics that hampered their identification and the distinction between Klebsiella and Enterobacter species. Ten isolates were identified as K. pneumoniae due to negative reactions for motility and inducible beta-lactamase test (ESBL and AmpC) despite being positive for ornithyne descarboxilase. These isolates were genotyped by ribotyping and polymerase chain reaction (PCR) with repetitive extragenic palindromic sequences (REP). Ribotyping by means of an automated instrument and EcoRI and Pvu II as restriction enzymes resulted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; REP-PCR; Genetic variation; Ornithinolytic.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200016

Resistance of Klebsiella Pneumoniae clinical isolates: linkage of outer membrane proteins (omps) with production esbls
Provedor de dados: Brazilian Journal of Microbiology
Autores: Marques,Lívia Érika Carlos; Oliveira,Danielle Ferreira de; Marques,Márcia Maria Mendes; Silva,Ana Raquel Araújo da; Alves,Carlucio Roberto; Guedes,Maria Izabel Florindo.
Three isolates of Klebsiella pneumoniae, collected from the University Hospital in Fortaleza, Brazil, were analyzed to determine their resistance to multiple antibiotics. The results of this study showed that the resistance of the clinically isolated bacteria is associated with the production of extended-spectrum beta-lactamases (ESLBs) and loss of outer membrane proteins.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Β-lactamase and porin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200009

Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae
Provedor de dados: Brazilian Journal of Microbiology
Autores: Mobarak-Qamsari,Maryam; Ashayeri-Panah,Mitra; Eftekhar,Freshteh; Feizabadi,Mohammad Mehdi.
The present study describes integron mediated multiple antibiotic resistance in extended-spectrum β-lactamase producing clinical isolates of Klebsiella pneumoniae. One hundred and four clinical isolates of K. pneumoniae from two Iranian hospitals were screened for extended-spectrum β-lactamase production and susceptibility of the extended-spectrum β-lactamase producing isolates was determined to 17 antibiotics by disc diffusion. Presence of integron classes 1, 2 and 3 was detected by PCR and integrase specific primers. Isolates harboring class 1 integron were then screened for variable regions using PCR. Fifty isolates (48%) produced extended-spectrum β-lactamases among which, 22 (44%) harbored class 1, 3 (6%) carried class 2 and none contained class 3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Extended-spectrum β-lactamase; Multiple antibiotic resistance; Integron.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300028

Molecular characterization of multidrug-resistant Klebsiella pneumoniae isolates
Provedor de dados: Brazilian Journal of Microbiology
Autores: Hou,Xiang-hua; Song,Xiu-yu; Ma,Xiao-bo; Zhang,Shi-yang; Zhang,Jia-qin.
Klebsiella pneumoniae is an important cause of healthcare-associated infections worldwide. Selective pressure, the extensive use of antibiotics, and the conjugational transmission of antibiotic resistance genes across bacterial species and genera facilitate the emergence of multidrug-resistant (MDR) K. pneumoniae. Here, we examined the occurrence, phenotypes and genetic features of MDR K. pneumoniae isolated from patients in intensive care units (ICUs) at the First Affiliated Hospital of Xiamen University in Xiamen, China, from January to December 2011. Thirty-eight MDR K. pneumoniae strains were collected. These MDR K. pneumoniae isolates possessed at least seven antibiotic resistance determinants, which contribute to the high-level resistance of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Multidrug resistance; Molecular characterization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300759

Isolation and characterization of arsenic resistant bacteria from wastewater
Provedor de dados: Brazilian Journal of Microbiology
Autores: Abbas,Syed Zaghum; Riaz,Mehwish; Ramzan,Naseem; Zahid,M. Tariq; Shakoori,Farah R.; Rafatullah,Mohd.
The present study proposed the isolation of arsenic resistant bacteria from wastewater. Only three bacterial isolates (MNZ1, MNZ4 and MNZ6) were able to grow in high concentrations of arsenic. The minimum inhibitory concentrations of arsenic against MNZ1, MNZ4 and MNZ6 were 300 mg/L, 300 mg/L and 370 mg/L respectively. The isolated strains showed maximum growth at 37 ºC and at 7.0 pH in control but in arsenite stress Luria Bertani broth the bacterial growth is lower than control. All strains were arsenite oxidizing. All strains were biochemically characterized and ribotyping (16S rRNA) was done for the purpose of identification which confirmed that MNZ1 was homologous to Enterobacter sp. while MNZ4 and MNZ6 showed their maximum homology with Klebsiella...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arsenite oxidizing bacteria; Bioremediation; Enterobacter sp.; Klebsiella pneumoniae.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400022

KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Biberg,Camila Arguelo; Rodrigues,Ana Claudia Souza; Carmo,Sidiane Ferreira do; Chaves,Claudia Elizabeth Volpe; Gales,Ana Cristina; Chang,Marilene Rodrigues.
The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Carbapenems; Drug-resistant bacteria.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501

Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process
Provedor de dados: Brazilian Journal of Microbiology
Autores: Fesharaki,Parisa Jafari; Nazari,Pardis; Shakibaie,Mojtaba; Rezaie,Sassan; Banoee,Maryam; Abdollahi,Mohammad; Shahverdi,Ahmad Reza.
The use of biologically derived metal nanoparticles for various proposes is going to be an issue of considerable importance; thus, appropriate methods should be developed and tested for the biological synthesis and recovery of these nanoparticles from bacterial cells. In this research study, a strain of Klebsiella pneumoniae was tested for its ability to synthesize elemental selenium nanoparticles from selenium chloride. A broth of Klebsiella pneumoniae culture containing selenium nanoparticles was subjected to sterilization at 121ºC and 17 psi for 20 minutes. Released selenium nanoparticles ranged in size from 100 to 550 nm, with an average size of 245 nm. Our study also showed that no chemical changes occurred in selenium nanoparticles during the wet...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Selenium nanoparticles; Sterilization; Klebsiella pneumoniae; Synthesis; Recovery.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200028

Carbapenem and cefoxitin resistance of Klebsiella pneumoniae strains associated with porin OmpK36 loss and DHA-1 β-lactamase production
Provedor de dados: Brazilian Journal of Microbiology
Autores: Shi,Weifeng; Li,Kun; Ji,Yun; Jiang,Qinbo; Wang,Yuyue; Shi,Mei; Mi,Zuhuang.
Clinical isolates of carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae) strains are being increased worldwide. Five pan-resistant K. pneumoniae strains have been isolated from respiratory and ICU wards in a Chinese hospital, and reveal strong resistance to all β-lactams, fluoroquinolones and aminoglycosides. Totally 27 β-lactamase genes and 2 membrane pore protein (porin) genes in 5 K. pneumoniae strains were screened by polymerase chain reaction (PCR). The results indicated that all of 5 K. pneumoniae strains carried blaTEM-1 and blaDHA-1 genes, as well as base deletion and mutation of OmpK35 or OmpK36 genes. Compared with carbapenem-sensitive isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the resistant isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; AmpC β-Lactamase; Porin; Molecular biology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200015

Extended-spectrum β-lactamases producing Klebsiella pneumoniae isolated in two hospitals in Goiânia/Brazil: detection, prevalence, antimicrobial susceptibility and molecular typing
Provedor de dados: Brazilian Journal of Microbiology
Autores: Santos,Daniella Fabíola dos; Pimenta,Fabiana Cristina; Alves,Rodrigo; Montalvão,Edlaine Rodrigues; Santos,Daniela Braz dos; Carmo Filho,José Rodrigues do.
This study was developed to evaluate the prevalence of extended-spectrum β-lactamases (ESBL) producing Klebsiella pneumoniae in two hospitals (A and B) in Goiânia, GO, Brazil. The antimicrobial susceptibility of the isolates was determined using the MicroScan WalkAway (Dade Behring, USA). Tests to evaluate the genetic correlation between the isolates were also performed. For the ESBL phenotypic test, the Double-disk diffusion (DD) method was used. The strainsisolated in Hospital B were submitted to DNA analysis by pulsed-field gel electrophoresis (PFGE). The study showed high prevalence of ESBL-producing K. pneumoniae (25% in hospital A and 66.7% in hospital B), with high rates of antimicrobial resistance. The most active compound was imipenem (100%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extended-spectrum β-lactamases (ESBL); Klebsiella pneumoniae; Β-lactamases; Bacterial resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400002

Detection and analysis of different interactions between resistance mechanisms and carbapenems in clinical isolates of Klebsiella pneumoniae
Provedor de dados: Brazilian Journal of Microbiology
Autores: Dalmolin,Tanise Vendruscolo; Bianchini,Bianca Vendruscolo; Rossi,Grazielle Guidolin; Ramos,Ana Carolina; Gales,Ana Cristina; Trindade,Priscila de Arruda; Campos,Marli Matiko Anraku de.
Abstract Carbapenems are considered last-line agents for the treatment of serious infections caused by Klebsiella pneumoniae, and this microorganism may exhibit resistance to β-lactam antibiotics due to different mechanisms of resistance. We evaluated 27 isolates of K. pneumoniae resistant to carbapenems recovered from inpatients at the University Hospital of Santa Maria-RS from July 2013 to August 2014. We carried out antimicrobial susceptibility, carbapenemase detection, testing for the presence of efflux pump by broth microdilution and loss of porin by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Genetic similarity was evaluated by ERIC-PCR. High levels of resistance were verified by the minimum inhibitory concentration for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Carbapenemases; Loss of porins; Efflux pump.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300493