Consulta: subjectFacets:"LD50"
Registros recuperados: 9
Data/hora: 04/06/2024 07:32:58

METHODOLOGICAL CARE IN THE EVALUATION OF THE LD50 AND OF THE NEUTRALIZATION OF THE LETHAL EFFECT OF Crotalus durissus terrificus VENOM BY THE PLANT Peschiera fuchsiaefolia (APOCYNACEAE)
Provedor de dados: 79
Autores: BATINA,M. de F. C.; GIGLIO,J. R.; SAMPAIO,S. V.
In the present study, we demonstrate that the volumes in which a given protein mass of Crotalus durissus terrificus venom or of a lyophilized stabilized aqueous extract (LSAE) of Peschiera fuchsiaefolia, an antivenom agent injected intramuscularly, have a decisive influence on the results. The LD50 of C. d. terrificus venom injected i.m. in a final volume of 200µl (2µl/g) (saline solution, 0.9% NaCl) was 180µg/100g rat body weight (p&amp;lt;0.05, 161 to 202µg/100g body weight) and the LD50 of the venom injected i.m. in a volume of 50µl (0.5µl/g) was 120µg/100g body weight (p&amp;lt;0.05, 107 to 134 µg/100g body weight). The reduction of the final volume injected i.m. also required a reduced mass of LSAE necessary to neutralize the lethal effect of C. d....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LD50; Peschiera fuchsiaefolia; Crotalus durissus terrificus; Snake venom antidotes.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000100003

Effects of the European hornet ( Vespa crabro Linnaeus 1761) crude venom on its own species
Provedor de dados: 80
Autores: Nadolski,Jerzy.
Background Lethal dose 50% is a classical index of toxicity that usually employs small rodents as experimental animals. Therefore, scarce data are available on the effects of venom on invertebrates, particularly the impact of wasp venom on its own species. Findings In the present study, the lethality of Vespa crabro venom on its own species was studied. Lethal dose 50% values of crude venom on workers of hornet Vespa crabro were estimated to be 4.0 mg/kg of body weight. Conclusions Wasps can use their venom apparatus effectively when attacking foreign workers that appear in the immediate vicinity of their nest. The toxins released during stinging are potent enough to kill. The result of this study eliminates the popular myth that venomous animals can be...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: European hornet; Vespa crabro; Hornet venoms; LD50.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100401

Experimental Tityus serrulatus scorpion envenomation: age- and sex-related differences in symptoms and mortality in mice
Provedor de dados: 80
Autores: MB,Pucca; EC,Roncolato; LB,Campos; FS,Fernandes; GR,Mendes; TB,Bertolini; FA,Cerni; JE,Barbosa.
Among the various methods for evaluating animal venom toxicity, the calculation of the median lethal dose (LD50) is the most widely used. Although different protocols can be used to calculate the LD50, the source of the venom and the method of extraction, as well as the strain, age, and sex of the animal model employed, should be taken into consideration. The objective of the present study was to evaluate the influence of sex and age on the toxicity of Tityus serrulatus scorpion venom in Swiss mice. Although the symptoms of envenomation were similar in male and female animals, female mice proved to be more resistant to the venom. In females, age had no impact on the susceptibility to scorpion envenomation. Male mice were more sensitive to T. serrulatus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpions; LD50; Stings; Age factors; Gender.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300013

In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran
Provedor de dados: 80
Autores: Salmanizadeh,Hossein; Babaie,Mahdi; Zolfagharian,Hossein.
Background The venom of the family Viperidae, including the saw-scaled viper, is rich in serine proteinases and metalloproteinases, which affect the nervous system, complementary system, blood coagulation, platelet aggregation and blood pressure. One of the most prominent effects of the snake venom of Echis carinatus (Ec) is its coagulation activity, used for killing prey. Materials and methods Subfractions F1A and F1B were isolated from Ec crude venom by a combination of gel chromatography (Sephadex G-75) and ion exchange chromatography on a DEAE-Sepharose (DE-52). These subfractions were then intravenously (IV) injected into NIH male mice. Blood samples were taken before and after the administration of these subfractions. Times for prothrombin, partial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Procoagulant activity; Blood coagulation; Echis carinatus; Chromatography; LD50.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100302

Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production
Provedor de dados: 80
Autores: Nalbantsoy,A; Karabay-Yavasoglu,NU; Sayim,F; Deliloglu-Gurhan,I; Gocmen,B; Arikan,H; Yildiz,MZ.
The venomous Levantine viper, Macrovipera lebetina lebetina is endemic to Cyprus. The objective of this study was to investigate in vitro cytotoxicity, in vivo lethality, and antivenom production followed by a re-immunization schedule in mice against Macrovipera lebetina lebetina venom. The LD50 value was estimated as 7.58 mg/kg within 24 hours by different venom doses administrated intraperitoneally in mice. Freund's complete and incomplete adjuvants were used for first and second immunization of mice in antivenom production. A cell-based assay was performed to determine the effects of Macrovipera lebetina lebetina venom and antivenom neutralizing potency on L929 cell viability. The snake venom toxicity and cytotoxicity were examined and comparison of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrovipera lebetina lebetina; LD50; Lethality; Snake venom; Antivenom.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200011

Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
Provedor de dados: 80
Autores: Duan,Hai-long; He,Qi-yi; Zhou,Bin; Wang,Wen-wen; Li,Bo; Zhang,Ying-zheng; Deng,Qiu-ping; Zhang,Ying-feng; Yu,Xiao-dong.
Abstract  Background  Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom antibody (immunoglobulin Y - IgY) from leghorn chickens.  Methods  IgY in egg yolk from white leghorn chicken previously injected with T. albolabris venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified by SDS-PAGE, ELISA and Western blot, and its neutralizing assay was conducted on mice.  Results  Chickens injected multiple times...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgY; Egg yolk; Snake venom; Trimeresurus albolabris; LD50.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100316

Evaluation of Fenoxaprop-P-Ethyl Resistant Littleseed Canarygrass (Phalaris minor) in Pakistan
Provedor de dados: 87
Autores: ABBAS,T.; NADEEM,M.A.; TANVEER,A.; AHMAD,R.
ABSTRACT To test resistance status of Phalaris minor (littleseed canary grass) to fenoxaprop-P-ethyl in Pakistan, a field survey was conducted during 2014. Uncontrolled P. minor plants were selected for seed collection from wheat fields where fenoxaprop-P-ethyl was used to control P. minor. Seeds were collected from eight different locations near Fasialabad, Pakistan. Susceptible plants were also selected near Faisalabad having no history of fenoxaprop-P-ethyluse for comparison. These seeds were grown in pots for resistance confirmation using completely randomized design with factorial arrangement having four replicates. Four doses of fenoxaprop-P-ethylcontrol (0X), 46.9 (0.5X), 93.7 (1X) and 187 (2X) g a.i. ha-1 were sprayed at 3 to 4 leaf stage of P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACCase-inhibiter; LD50; Phalaris minor; Herbicide resistance; Resistance index; Survey.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000400833

การติดตามความต้านทานต่อสารเคมีป้องกันกำจัดแมลงของเพลี้ยกระโดดสีน้ำตาลในภาคตะวันออกเฉียงเหนือของไทย
Provedor de dados: 106
Autores: Sukanya Arunmit; Wantana Sriratanasak; Jintana Chaiwong; Urassaya Boonpramook.
Brown planthopper (BPH), Nilaparvata lugens (Stål), is a major insect pest of rice in Thailand.Because of their ability to adapt to host plant and environment, the BPH becomes an extremely destructive pest and difficult to control. The purpose of this research is to monitor the insecticide resistance of BPH in North Eastern (NE) Thailand. The BPH populations were collected from the outbreak areas in 9provinces during 2011 to 2012. The toxicity (LD50) of 10 insecticides was conducted using Topical bioassay method. The results showed that the BPH populations from 7 provinces were resistant to ethiprole and isoprocarb. The NongBua Lam Phu population was the widest spectrum of resistance to the tested insecticides,whereas, the BPH population from Sisaket was...
Tipo: PhysicalObject
Palavras-chave: Rice; BHP; Insecticide resistance; Toxicity level; LD50; North eastern; Thailand; ข้าว; เพลี้ยกระโดดสีน้ำตาล; ความต้านทานต่อสารเคมีป้องกันกำจัดแมลง; ระดับความเป็นพิษ; LD50; ภาคตะวันออกเฉียงเหนือ; ประเทศไทย.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5627

Acute Contact Toxicity Test of Oxalic Acid on Honeybees in the Southwestern Zone of Uruguay
Provedor de dados: 117
Autores: Carrasco-Letelier,Leonidas; Mendoza,Yamandu; Ramallo,Gustavo.
This work studies the acute contact toxicity of oxalic acid (OA) on a honeybee polyhybrid subspecies (Apis mellifera), which is the dominant biotype in southwestern zone of Uruguay (SWZU) and the country's most important honey-producing region. We determined the mean lethal dose (LD50), as well as the no observed effect level (NOEL) and the lowest observed effect level (LOEL) values. We also estimated the total number of honeybees per hive in the test area. The aim was to assess the relationship between the maximum OA dose used in Uruguay (3.1 g OA per hive) and the toxicological parameters of honeybees from SWZU. The current dose of 3.1 g OA per hive corresponds to 132.8 OA per honeybee since determined NOEL is 400 OA per honeybee; our results indicate...
Tipo: Journal article
Palavras-chave: LD50; Uruguay.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000200019