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Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment
Provedor de dados: Brazilian Journal of Microbiology
Autores: Oliveira,I.C.; Almeida,R.C.C.; Hofer,E.; Almeida,P.F.
A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 10(8) pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria; Bacteriophage A511; Laser light; Detection; Skimmed milk.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300040