Consulta: subjectFacets:"Leptospira"
Registros recuperados: 29
Data/hora: 07/06/2024 17:31:55

Anti-leptospirose agglutinins in equine sera, from São Paulo, Goias, and Mato Grosso do Sul, Brazil, 1996-2001
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Langoni,H.; Da Silva,A.V.; Pezerico,S.B.; De Lima,V.Y.
Equine leptospirosis can present a non-symptomatic form, an acute clinical form, or even develop chronically, causing reproductive alterations, such as abortion and recurrent uveitis. Since the prevalence of leptospirosis in several countries and regions is widely reported, the objective of this study was to verify the prevailing equine leptospirosis in different regions of Brazil. Sera from 1402 blood samples from horses of different age, sex, breed, and purpose were examined. These samples came from southeastern and central west states of Brazil. The method utilized was the Microscopic Agglutination Test (MAT), with 12 different Leptospira serovars. From the sera tested, 754 (54%) were positive for one (385) or more (372) serovars. These results were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Horse; Serology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300003

Response activity of alveolar macrophages in pulmonary dysfunction caused by Leptospira infection
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Marinho,M.; Oliveira-Júnior,I. S.; Perri,S. H.V; Peiró,J. R.; Pavanelli,T. F.; Salomão,R.
Leptopspirosis is a syndrome with different clinical manifestations including the most severe and often fatal forms of pulmonary disease of unknown etiology. Pulmonary injury during the inflammatory process has been associated with the excessive number of alveolar macrophages (AMs) and polymorphonuclear leukocytes stimulated in the lungs and with the production of reactive oxygen and nitrogen intermediates and other inflammatory mediators. The aim of the present work was to evaluate the cellular immune response of AMs or inflammatory cells of hamsters during leptospirosis. The activity of AMs was determined by measuring nitric oxide (NO) and protein production as well as inflammatory cell infiltration in bronchoalveolar lavage (BAL) fluid. Pulmonary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alveolar macrophages; Bronchoalveolar lavage; Gasometry; Pulmonary dysfunction; Hamsters; Leptospira.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100005

Ovine leptospirosis in Brazil
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Lucheis,SB; Ferreira Jr.,RS.
Leptospirosis is a zoonosis distributed worldwide, endemic mainly in humid subtropical and tropical countries, with epidemic potential. It affects a range of both wild and domestic animals, including sheep, which transport leptospires in their urine and, therefore, can infect other animals and humans who deal with them. Therefore, leptospirosis is characterized as an occupational zoonosis. In individual herds leptospirosis can cause severe economic loss due to miscarriages and outbreaks of mastitis with a significant reduction of milk production. The disease is caused by Leptospira interrogans, which was reclassified into 13 pathogenic species, and distributed into more than 260 serovars classified into 23 serogroups. The clinical signs of infection may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sheep; Leptospira; Diagnosis; Animal diseases.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400006

Detección de Leptospira santarosai y L. kirschneri en bovinos: nuevos aislados con potencial impacto en producción bovina y salud pública
Provedor de dados: Revista Veterinaria México
Autores: Carmona-Gasca,Carlos Alfredo; León Lara,Lemuel; Castillo-Sánchez,Luz Olivia; Ramírez-Ortega,José Manuel; Ko,Albert; Luna Palomera,Carlos; Peña-Moctezuma,Alejandro de la.
La leptospirosis bovina causa grandes pérdidas económicas a la ganadería por problemas reproductivos y también es un riesgo de salud pública. En México, desde el siglo pasado se ha registrado la presencia de anticuerpos contra serovariedades de Leptospira por la técnica de aglutinación microscópica (AM), en bovinos y otras especies animales. En muy pocos casos, la enfermedad fue demostrada por el aislamiento de Leptospira, y en tales casos, su caracterización se basó en métodos inmunológicos lentos que requirieron la comparación con cepas de referencia y que fueron realizados fuera de México. En el presente trabajo se realizó la caracterización molecular mediante la secuenciación de locus múltiples (MLST), de aislados de Leptospira obtenidos de riñones de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Tarassovi; Mini; Grippotyphosa; Bovinos; Aislamiento; MLST.
Ano: 2011
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922011000400003

Isolamento de Leptospira interrogans de espécies selvagens de vida livre em regiões semiáridas do nordeste brasileiro
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Silva, F. J.; Silva, T. R.; Loffler, S. G.; Santos, C. E. P.; Brihuega, B.; Vasconcellos, S. A.; Mathias, L. A.
O objetivo do presente trabalho é descrever o isolamento de Leptospira interrogans de espécies selvagens de vida livre em regiões semiáridas do Nordeste brasileiro. Houve aprovação da Comissão de Ética no Uso de Animais, protocolo 027958/10. Entre fevereiro e março de 2012, em pequenas propriedades rurais de Sobral, CE, e Sobradinho, BA, foram colhidas amostras de urina de um Cavea aperea, dois Euphractus sexcinctus e um Cerdocyon thous. A pesquisa de leptospiras foi efetuada por cultivos de amostras de urina semeadas nos meios de Fletcher e de Ellinghausen - McCullough - Johnson - Harris (EMJH). Os cultivos apresentando crescimento de leptospiras foram encaminhados ao Instituto Nacional de Tecnologia Agropecuária, Buenos Aires, Argentina, e as estirpes de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caatinga; Leptospira; MLVA.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/32178

Identificação de cães portadores de leptospiras patogênicas mantidos em abrigos coletivos
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Miotto, B. A.; Guilloux, A. G. A.; Hora, A. S.; Tozzi, B. F.; Penteado, M. S.; Hagiwara, M. K.
A leptospirose é uma zoonose de distribuição cosmopolita causada pela infecção de bactérias patogênicas do gênero Leptospira. Cães podem tornar-se portadores, eliminando leptospiras pela urina de forma assintomática. Quando albergados em abrigos coletivos, podem atuar inadvertidamente como fonte de infecção para outros cães, tratadores e adotantes. Esse estudo foi delineado para investigar a presença de cães portadores de leptospiras patogênicas mantidos em abrigos coletivos. Foram coletadas amostras de urina e sangue de 92 cães albergados no canil da Prefeitura do Campus Universitário da USP, São Paulo, entre julho e dezembro de 2013. Os cães foram avaliados por qPCR quanto à presença de leptospiras em amostras de urina e sangue por meio da amplificação...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cães; Leptospira; Abrigo para animais.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/32174

Isolamento de leptospira borgpetersenii de cebus apella em propriedade rural de agricultura familiar da Chapada dos Guimarães, MT
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Silva, F. J.; Santos, C. E. P.; Loffler, S. G.; Silva, T. R.; Brihuega, B.; Vasconcellos, S. A.; Mathias, L. A.
Primatas não humanos de vida livre são importantes fontes de infecção de leptospiras para humanos e animais domésticos. Por serem astutos, suas capturas são difíceis e pouco frequentes, situação que oferece especial desafio ao estudo de isolamento de leptospiras. O objetivo do trabalho é descrever um caso de isolamento de Leptospira borgpetersenii de um espécime de Cebus apella. Houve aprovação da Comissão de Ética no Uso de Animais, protocolo 027958/10. Por cistocentese, foi realizada a colheita de urina de um macho adulto capturado em uma pequena propriedade rural da Chapada dos Guimarães, MT, em agosto de 2012. A pesquisa de Leptospira spp. na urina foi efetuada por cultivos nos meios de Fletcher e de Ellinghausen - McCullough - Johnson - Harris (EMJH)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; MLVA; Primatas.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/32177

Casos de leptospirose em bovinos de leite em uma propriedade da região nordeste do Ceará
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Moura, Gabriela Hemylin Ferreira; Lima, Mikael Almeida; Gadelha, Ivana Cristina Nunes.
O objetivo deste trabalho foi relatar a ocorrência de reações sorológicas para os sorovares de Leptospira Tarassovi Castellonis em bovinos leiteiros da região nordeste do Ceará, tendo em vista inexistência de relatos nesta região. A leptospirose é uma zoonose bacteriana causada por espiroquetas do gênero Leptospira, sendo sua distribuição geográfica cosmopolita, predominante em clima tropical e subtropical, tendo maior incidência em períodos com altos níveis pluviométricos. Foram atendidos dois bovinos de uma propriedade da região, no município de Limoeiro do Norte, fêmeas, com aproximadamente quatro anos de idade e produtoras de leite. Destes animais foram coletadas amostras de 5mL de sangue, por punção da veia jugular utilizando-se agulhas descartáveis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Tarassovi; Castellonis.
Ano: 2014
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/23751

Anticorpos aglutinantes pós-vacinais antileptospirose em rebanhos de bovinos e equinos naturalmente infectados por Leptospira spp
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Martins, G.; Loureiro, A. P.; Narduche, L.; Correia, L.; Pinto, P.; Libonati, H.; Grapiglia, J.; Cavalcante, E.; Oliveira, C.; Lilenbaum, W.
A vacinação sistemática contra a leptospirose dos rebanhos de bovinos e equinos é um recurso profilático indicado para a redução dos agravos relacionados a essa infecção e dos prejuízos econômicos que ela determina. Nesse contexto, o presente trabalho investigou os níveis de anticorpos aglutinantes induzidos pela vacinação contra a leptospirose em rebanhos de bovinos e equinos naturalmente infectados por Leptospira spp. Foram trabalhados dois rebanhos: um de bovinos (162 vacas) e um de equinos (54 éguas), ambos acometidos por leptospirose. Os animais foram divididos em grupos vacinados e não vacinados e foram utilizadas vacinas comerciais, de duas marcas em bovinos e uma em equinos. Foram efetuadas colheitas de sangue e urina durante 120 dias para...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Bovinos; Equinos.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/32170

Pesquisa serológica de Leptospira en roedores silvestres, bovinos, equinos y caninos en el noreste de México
Provedor de dados: Revista de Salud Animal
Autores: Méndez,C; Benavides,L; Esquivel,A; Aldama,A; Torres,J; Gavaldón,D; Meléndez,P; Moles,L.
La estrecha convivencia de las especies domésticas con los roedores silvestres en ecosistemas modificados, hace relevante estudiar la participación de estos en el mantenimiento de distintas serovariedades de Leptospira. El propósito de este trabajo fue determinar la distribución de anticuerpos antileptospira en roedores silvestres y en tres especies domésticas que comparten el mismo hábitat en un área endémica de leptospirosis bovina al NE de México. Se determinaron anticuerpos antileptospira en roedores, bovinos, equinos y caninos. Se analizaron muestras de suero de 24 roedores, 220 bovinos, 24 equinos y 6 caninos con la prueba de aglutinación microscópica empleando 12 serovariedades de Leptospira de las más comunes en México ; excepto los roedores que se...
Tipo: Journal article
Palavras-chave: Leptospira; Anticuerpos antileptospira; Roedores; Bovinos; Equinos; Caninos.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000100004

LA LEPTOSPIROSIS CANINA Y SU PROBLEMÁTICA EN MÉXICO
Provedor de dados: Revista de Salud Animal
Autores: Luna,A.M.A; Moles,C.L.P; Gavaldón,R.D; Nava,V.C; Salazar,G.F.
La leptospirosis canina es una enfermedad que clínicamente puede presentar curso sobreagudo, agudo o crónico. Es de difícil diagnóstico por la gran variedad de manifestaciones clínicas, ya que no involucra necesariamente ictericia, se manifiesta principalmente en forma subclínica, por lo que son más frecuentes los hallazgos serológicos, que la manifestación de la enfermedad. En casos crónicos, puede ocasionar la muerte por insuficiencia renal y hepática. Los animales recuperados quedan en estado de portador en el que diseminan a la bacteria a través de la orina, representando un factor de riesgo y un importante eslabón en la cadena epidemiológica de la leptospirosis humana. La situación de la leptospirosis canina en México es poco conocida y es conveniente...
Tipo: Journal article
Palavras-chave: Leptospira; Leptospirosis; Perros; Canideos; Canicola; Icterohaemorrhagiae.
Ano: 2008
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000100001

Freqüência de aglutininas anti-Leptospira interrogans em eqüídeos, em Minas Gerais, 2003 a 2004
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Chiareli,D.; Moreira,E.C.; Gutiérrez,H.O.D.; Rodrigues,R.O.; Marcelino,A.P.; Meneses,J.N.C.; Almeida,V.M.A.
The goal of this research was to find the frequency and spatial distribution of infection caused by Leptospira interrogans in equidae in Minas Gerais State from September 2003 to March 2004. Samples of blood serum (6,475) were analyzed by microscopic agglutination test. From the total, 381 samples were positive (5.9 %), with title equal or superior to 1:200 for one or more serovars of leptospira. The most frequent serovars were Hardjo (Norma), Pomona, Bratislava, and Batavie. The higher frequency of equidae reagents were recorded at the North and Northeast region of Minas Gerais, followed by Triângulo Mineiro and Alto Paranaíba, then Central, West, Metropolitan area of Belo Horizonte, and South/Southwest.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eqüideos; Leptospira; Freqüência; Distribuição espacial.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600043

Culture and molecular identification of microorganisms from Digital Dermatitis lesions in dairy cattle: Leptospira, an unexpected finding
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Diniz,S.; Sandes,S.H.C.; Bomfim,M.R.Q.; Santos,P.C.; Cruz,F.D.; Moreira,T.F.; Carvalho,M.R.S.; Cisalpino,P.S.; Moreira,E.S. A.
ABSTRACT Bovine digital dermatitis (BDD) is an infectious and contagious disease characterized by ulcerative and proliferative lesions affecting the skin on the bulbs of the heel or the interdigital cleft in dairy cattle, often associated with lameness. Evidences on the etiology of BDD indicate that it is multifactorial, involving environmental factors and multiple bacterial colonization. We isolated and identified microorganisms from BDD biopsy samples obtained from five Holstein Friesian and two Jersey cows by cultivation and molecular identification of bacterial isolates using 16S rRNA gene sequence analysis. We identified six bacterial species: Spirochetes as Treponema pedis and Leptospira broomi/L. fainei, L. licerasiae/L. wolffii; Corynebacterium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine digital dermatitis; Molecular identification; 16S rRNA gene analysis; Treponema; Leptospira.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300559

Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
Provedor de dados: Biological Research
Autores: SCHREIER,STEFAN; TRIAMPO,WANNAPONG; DOUNGCHAWEE,GALAYANEE; TRIAMPO,DARAPOND; CHADSUTHI,SUDARAT.
Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for the establishment of standard curves. They were found to be linear and slightly different in gradient for each serovar. The ease, rapidity, and reliability of these two adapted and optimized counting techniques may provide a useful alternative enumeration technique...
Tipo: Journal article
Palavras-chave: Leptospirosis; Leptospira; Enumeration; Spectroscopy; Indirect counting.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100001

Detection of Leptospira spp. from pure cultures and from experimentally contaminated bovine semen by polymerase chain reaction
Provedor de dados: Brazilian Jounarl of Veterinary
Autores: HEINEMANN,Marcos Bryan; GARCIA,José Fernando; NUNES,Cáris Maroni; HIGA,Zenaide Maria Moraes; VASCONCELLOS,Silvio Arruda; Richtzenhain,Leonardo José.
Due to the high importance of the venereal transmission of bovine leptospirosis, this study aimed to test the ability of PCR to detect Leptospira interrogans serovar hardjo DNA in experimentally contaminated bovine semen. Employing primers directed to the 16S rRNA gene, 10 bacteria/ml of semen could be detected by PCR. Results achieved in this work show that PCR can have a great potential to detect Leptospira spp. in insemination centers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Leptospira; Semen; Polimerase Chain Reaction; Bovine.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000100002

Baypamun® action in hamsters experimentally infected with Leptospira interrogans serogroup Canicola
Provedor de dados: Brazilian Jounarl of Veterinary
Autores: LANGONI,Hélio; SEABRA JÚNIOR,Rui; CABRAL,Kenio Gouveia; CUNHA,Eva Laurice Pereira.
Baypamun® is an immunity modulator recommended as prophylactic and therapeutic use. In the present study, this product was used in the hamsters experimentally infected with Leptospira interrogans serogroup canicola. The animals were divided in 5 groups, with 20 animals each. This product was used as a therapeutic, prophylactic and as adjuvant. As a therapeutic use all the animals died. Despite of eight (40.0%) survivors, the prophylactic use wasn't also totally favorable due to leptospire recovering from kidneys of those animals. Based on the analysis of antibodies levels among animals that received vaccine and vaccine with Baypamun® as adjuvante, this product didn't conferred superior humoral answer. However, other studies should be accomplished utilizing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunomodulators; Leptospira; Immunity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000600010

Immune strategies using single-component LipL32 and multi-component recombinant LipL32-41-OmpL1 vaccines against leptospira
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Feng,C.Y.; Li,Q.T.; Zhang,X.Y.; Dong,K.; Hu,B.Y.; Guo,X.K.
Genes encoding lipoproteins LipL32, LipL41 and the outer-membrane protein OmpL1 of leptospira were recombined and cloned into a pVAX1 plasmid. BALB/c mice were immunized with LipL32 and recombined LipL32-41-OmpL1 using DNA-DNA, DNA-protein and protein-protein strategies, respectively. Prime immunization was on day 1, boost immunizations were on day 11 and day 21. Sera were collected from each mouse on day 35 for antibody, cytokine detection and microscopic agglutination test while spleen cells were collected for splenocyte proliferation assay. All experimental groups (N = 10 mice per group) showed statistically significant increases in antigen-specific antibodies, in cytokines IL-4 and IL-10, as well as in the microscopic agglutination test and splenocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immune strategy; Recombinant vaccine; Leptospira; Lipoproteins LipL32; LipL41.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900005

Isolation of leptospira Serovars Canicola and Copenhageni from cattle urine in the state of Paraná, Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Zacarias,Francielle Gibson da Silva; Vasconcellos,Silvio Arruda; Anzai,Eleine Kuroki; Giraldi,Nilson; Freitas,Julio Cesar de; Hartskeerl,Rudy.
In 2001, 698 urine samples were randomly collected from cattle at a slaughterhouse in the State of Paraná, Brazil. Direct examination using dark field microscopy was carried out immediately after collection. Five putative positive samples were cultured in modified EMJH medium, yielding two positive cultures (LO-14 and LO-10). Typing with monoclonal antibodies revealed that the two isolates were similar to Canicola (LO-14) and Copenhageni (LO-10). Microscopic agglutination test results show that Hardjo is the most common serovar in cattle in Brazil. Rats and dogs are the common maintenance hosts of serovars Copenhageni and Canicola. The excretion of highly pathogenic serovars such as Copenhageni and Canicola by cattle can represent an increasing risk for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Culture; Leptospirosis; Diagnosis; Cattle.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400028

Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Jung,Lenice Roteia Cardoso; Bomfim,Maria Rosa Quaresma; Kroon,Erna Geessien; Nunes,Álvaro Cantini.
Leptospires are usually classified by methods based on DNA-DNA hybridization and the conventional cross-agglutination absorption test, which uses polyclonal antibodies against lipopolysaccharides. In this study, the amplification of the rpoB gene, which encodes the beta-subunit of RNA polymerase, was used as an alternative tool to identify Leptospira. DNA extracts from sixty-eight serovars were obtained, and the hypervariable region located between 1990 and 2500-bp in the rpoB gene was amplified by polymerase chain reaction (PCR). The 600-bp amplicons of the rpoB gene were digested with the restriction endonucleases TaqI, Tru1I, Sau3AI and MslI, and the restriction fragments were separated by 6% polyacrylamide gel electrophoresis. Thirty-five fragment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; RpoB gene; RFLP; Serovar; DNA typing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200465

Human leptospirosis: occurrence of serovars of Leptospira spp. in the state of Minas Gerais, Brazil, from 2008 to 2012
Provedor de dados: Brazilian Journal of Microbiology
Autores: Oliveira,Marluce Aparecida Assunção; Leal,Élida Aparecida; Correia,Max Assunção; Serufo Filho,José Carlos; Dias,Ricardo Souza; Serufo,José Carlos.
Abstract  Background: Leptospirosis is an infectious and acute disease caused by Leptospira spp. that have high epidemic potential. This study verified the main Leptospira spp. serovars detected by MAT from serum of patients with suspicion of leptospirosis from 2008 to 2012 in Minas Gerais State.  Methods: The laboratory received sera from 4654 patients. All serum were screened by IgM-ELISA according to the manufacturer's instructions. Each sample reactive or indeterminate were tested against twenty-four serovars of Leptospira by MAT.  Results: In this study, 597 patients were classified as reactive on MAT. Only 301 patients were confirmed by laboratory test. It was not possible confirmation by laboratory diagnosis of 296 patients. Among the samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Serovar; Seroprevalence; Epidemiology; MAT.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300483

Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry
Provedor de dados: Brazilian Journal of Microbiology
Autores: Karcher,Daniel; Grenfell,Rafaella C.; Moreno,Andrea Micke; Moreno,Luisa Zanolli; Vasconcellos,Silvio Arruda; Heinemann,Marcos B.; Almeida Junior,Joao N. de; Juliano,Luiz; Juliano,Maria A.
ABSTRACT Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e.g., Leptospira interrogans and Leptospira kirschneri) may not be discriminated using this technology. Herein, we report an in-house Leptospira reference spectra database using Leptospira reference strains that were validated with a collection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Brazil; Identification; Mass spectrometry; MALDI-TOF.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400900

Cross neutralizing antibodies in hamsters vaccinated with leptospiral bacterins produced with three serovars of serogroup Sejroe
Provedor de dados: Brazilian Journal of Microbiology
Autores: Tabata,Rosana; Scanavini Neto,Hugo; Zuanaze,Marcelo Alexandre Fagnani; Oliveira,Eugenia Márcia de Deus; Dias,Ricardo Augusto; Morais,Zenaide Maria de; Ito,Fumio Honma; Vasconcellos,Silvio Arruda.
Three leptospiral bacterins, produced with different serovars of Serogroup Sejroe, namely the hardjo (bacterin A), wolffi (bacterin B) and guaricura (bacterin C), were evaluated in male hamsters (Mesocricetus auratus) by comparing the agglutinating and neutralizing antibodies titers using microscopic agglutination (MAT) and in vitro growth inhibition (GIT) tests. The immunization schedule was based on two 1.0 mL doses of non-diluted formalininactivated whole culture bacterin given through subcutaneous route with 10-day interval. The challenge was performed ten days after the second vaccine dose, when the animals were inoculated with 0.2 mL of non-inactivated cultures of each serovar through intraperitoneal route. On the 21st post-challenge day (PCD), all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Bacterins; Hamsters; Neutralizing antibodies; Serogroup Sejroe.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300016

Molecular characterization of the first leptospires isolated from goats in Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Lilenbaum,Walter; Kremer,Frederico; Ristow,Paula; Dellagostin,Odir; Bourhy,Pascale; Hartskeerl,Rudy; Vasconcellos,Silvio.
Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Serogrouping; Sequencing; PFGE; Rrs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050

Leptospirosis in slaughtered sows: serological and histopathological investigation
Provedor de dados: Brazilian Journal of Microbiology
Autores: Delbem,Ádina Cléia Botazzo; Freitas,Julio Cesar de; Bracarense,Ana Paula F. R. L.; Müller,Ernst Eckehardt; Oliveira,Rosângela Claret de.
Swine leptospirosis is recognized world wide as an important cause of reproductive failure. Serum and histo-pathological examinations have been constantly used to diagnose this disease. This study was carried out on slaughtered sows from Northern Paraná State to compare serum findings with kidney, liver, ovary and uterus histo-pathological examinations. The microscopic serum-agglutination carried out on 36 animals detected 24 with titles &gt; 100 and 12 negative (&lt; 100). The histopathological examinations made on livers, kidneys, ovaries and uterus stained with hematoxylin-eosin, were carried out on 16 randomly chosen animals, 11 from the groups with &gt; 100 serological titles and five from the negative group. The results showed that all the 16 animals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Swine; Diagnostic.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200016

Production of reactive oxygen (H2O2) and nitrogen (NO) intermediates and TNF-α in mice genetically selected for high (H) and low (L) antibody response and experimentally infected with Leptospira serovar pomona
Provedor de dados: Brazilian Journal of Microbiology
Autores: Haanwinckel,Maria Cristina Santos; Oliveira,Silvio Luis de.
The aim of the present study was to evaluate the activity of macrophages, and the production of TNF-α and antibodies against experimental infection by Leptospira serovar Pomona in mice genetically selected for High (H) or Low (L) humoral immune response. To evaluate macrophagic activity, peritoneal and splenic lavages were performed for determination of oxygen (H2O2) and nitrogen (NO) intermediates. The production of the tumor necrosis factor (TNF-α) was investigated through bioassays in serum and homogenates of splenic and hepatic cells of control and infected animals, as was as specific antibodies production. The immune response against serovar Pomona in those lines, was characterized by high antibody production, especially in later periods of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunological aspects; Biozzi mice; Leptospira; Macrophages; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200041

Characterization of a virulent Leptospira interrogans strain isolated from an abandoned swimming pool
Provedor de dados: Brazilian Journal of Microbiology
Autores: Forster,Karine M.; Hartwig,Daiane D.; Seixas,Fabiana K.; McBride,Alan J.A.; Monte,Leonardo G.; Recuero,Ana Lúcia C.; Brod,Claudiomar S.; Hartleben,Cláudia P.; Amaral,Marta; Dellagostin,Odir A.
Pathogenic Leptospira spp. are the etiological agents of leptospirosis, an important disease of both humans and animals. In urban settings, L. interrogans serovars are the predominant cause of disease in humans. The purpose of this study was to characterize a novel Leptospira isolate recovered from an abandoned swimming pool. Molecular characterization through sequencing of the rpoB gene revealed 100% identity with L. interrogans and variable-number tandem-repeat (VNTR) analysis resulted in a banding pattern identical to L. interrogans serogroup Icterohaemorrhagiae, serovar Copenhageni or Icterohaemorrhagiae. The virulence of the strain was determined in a hamster model of lethal leptospirosis. The lethal dose 50% (LD50) was calculated to be two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Leptospirosis; Virulent; VNTR; RpoB.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100024

Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires
Provedor de dados: Brazilian Journal of Microbiology
Autores: Seyffert,Núbia; Silva,Éverton Fagonde da; Bermudez,Viviane Lorenzato; Brod,Claudiomar Soares.
In leptospirosis, the Microscopic Agglutination Test (MAT) is the basis of serological diagnosis and the most widely used. In our study, the MAT was applied to evaluate the influence of temperature, incubation time, antigen density and age of the culture in the agglutinability of Leptospires and the interference of these factors in diagnosis. Three serum samples with titers of 100, 800 and 25.600 to the serovar tande (local isolate) of canicola serogroup were used with different combinations among the four factors. There was a significant relation among all factors, specially between the antigen density and age of the culture.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Microscopic agglutination; Leptospirosis; Laboratorial diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500025

Levantamento sorológico da leptospirose em caprinos leiteiros no Rio Grande do Sul, Brasil
Provedor de dados: Ciência Rural
Autores: Schmidt,Verônica; Arosi,Alessandra; Santos,Airton da Rosa dos.
Um estudo sorológico para Leptospira spp. foi realizado em caprinos leiteiros de 15 municípios do Rio Grande do Sul, Brasil. Foram examinados 354 soros frente a 12 sorovares de Leptospira pela técnica da aglutinação microscópica. No momento da coleta do sangue, todos os animais examinados possuíam aspecto saudável e não apresentavam sinais clínicos sugestivos de leptospirose. Dos animais amostrados, 3,4% apresentaram título 100 ou maior. Os sorovares icterohaemorragiae, pomona e hardjo foram encontrados nos animais soropositivos. Estes resultados confirmam a presença da infecção de caprinos por Leptospira no Rio Grande do Sul e indicam a existência de mais de um sorovar predominantemente nesta espécie na região.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirose; Caprinos; Leptospira.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000400010

Aglutininas antileptospíricas em búfalos do Vale do Ribeira, Estado de São Paulo
Provedor de dados: Ciência Rural
Autores: Langoni,Hélio; Del Fava,Claudia; Cabral,Kenio de Gouvêa; Silva,Aristeu Vieira da; Chagas,Sérgio Aguiar Pacheco.
Foram estudadas aglutininas antileptospíricas em 403 amostras de soro de búfalos, provenientes de sete Municípios do Vale do Ribeira, Estado de São Paulo, coletadas no período de janeiro de 1992 a junho de 1993. Utilizou-se o teste de soroaglutinação microscópica, considerando-se positivas as amostras cujo título fosse igual ou superior a 100. O maior título encontrado foi 1600 para o sorovar bratislava (1 amostra), seguido de 800 para wolffi (4 amostras). Do total, 152 (37,7%) das amostras foram positivas, sendo que, dentre os sorovares testados, a prevalência em ordem decrescente foi: wolffi (68, 44,8%), icterohaemorrhagiae (51, 33,6%), hardjo (51, 33,6%), castellonis (25, 16,5%), djasiman (12, 7,9%), grippotyphosa (10, 6,6%), pomona (8, 5,2%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Soroaglutinação microscópica; Búfalos.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000200019