Consulta: subjectFacets:"Leukocytes"
Registros recuperados: 32
Data/hora: 08/06/2024 01:31:24

Effect of PHA and LPS on the Activity of Nonspecific Cytotoxic Cells and Macrophages of Rainbow Trout (Oncorhynchus mykiss)
Provedor de dados: Gayana
Autores: Gómez,Leonardo A; Cortés,Raúl; Smith,Carlos T.
This work studied the effect of the lectin phytohaemagglutinin (PHA) and bacterial lipopolysaccharide (LPS) on the activity of nonspecific cytotoxic cells (NCCs) and macrophages of peripheral blood in the rainbow trout. NCCs are cells of the innate immunity, homologues to Natural Killer (NK) cells of mammals, capable of eliminating cells infected with intracellular pathogens and also tumoral cells. Their lytic activity is mediated by the perforin/granzyme system and the Fas receptor-Fas ligand (FasR-FasL) system. Activity of NCCs was evaluated by their capacity to lyse HL-60 tumor cells. On the other hand, macrophages eliminate microorganisms by several mechanisms, including the production of reactive oxygen species (ROS) and nitric oxide (NO). Results...
Tipo: Journal article
Palavras-chave: Interleukins; Innate immunity; FasR-FasL; Lectins; Leukocytes.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382013000100007

FISH IMMUNE SYSTEM
Provedor de dados: Gayana
Autores: Olabuenaga,Susana E.
The defence against infections is the essential function of the immune system of all Vertebrates. Fish present an efficient and developed immunologic response and in the case of Teleosts, with some similarities to the higher vertebrates. It can be divided into two types: innate or non specific and adquired or specific, both with a humoral and cellular compartments. Their relative importance can change according to the age of fish and is always under the influence of different factors. The most relevant components are mentioned here. There are two factors in fishes which strongly influence the immune response and antibody production, seasonal changes and temperature. The main lymphoid organs are described here: the thymus, the kidney and the spleen, as well...
Tipo: Journal article
Palavras-chave: Antigen; Antibody; Leukocytes; Phagocytosis; Lymphokines; Inflammation; Immunostimulants.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200010

Les parasites d'Arnoglossus laterna (Walbaum, 1792), Bothidae, dans le golfe du Lion (cote francaise de la Mediterranee) et leur influence sur les formules leucocytaires des poissons hotes
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Douellou, L; Bastide-guillaume, C; Romestand, B; Trilles, J.
Arnoglossus laterna is only infested by intestinal parasites. Each parasite distribution is independent of the distribution of other parasites and also independent of the sex of host. When a fish is infested by a great number of the two helminths Acanthocephaloides and Bothriocephalus clavibothrium , the proportion of lymphocytes tends to decrease and the proportion of neutrophils tends to increase (inflammatory response). Important infestation by Scolex pleuronectis induces an increase of the thrombocyte count for 100 counted leucocytes. After infestation by the nematode Thynnascaris sp. alone or with another parasite, even by small number, the proportion of lymphocytes decreases and the proportion of neutrophils increases.
Tipo: Text
Palavras-chave: Arnoglossus laterna; Leukocytes; Hematology; Parasitic diseases; Parasitism.
Ano: 1985
URL: http://archimer.ifremer.fr/doc/1985/publication-1767.pdf

Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration
Provedor de dados: International Journal of Morphology
Autores: Deveci,Engin; Deveci,Ienay.
Leukocyte-endothelial cell interactions have a pivotal role in immune responses. In our study Ten adult wistar albino rats weighing 180-200 g were used to obtain tissue samples. The animals were sacrificed by decapitation under ether anesthesia. The lymph nodes of the animals were then quickly removed. Specimens were immersed in the zinc iodide-osmium tetroxide (ZIO) solutation by Niebauer et al. (1969) and kept in the dark for 2 hours in this solution from temperature for fixation/staining. Samples were processed according to routine plastic embedding tecnique. Semithin sections of 1µm thick were cut and stained with methilene blue-azure II for light microscopic examination. We used zinc iodide-osmium tetroxide staining tecnique to distinguish endothelial...
Tipo: Journal article
Palavras-chave: Leukocytes; Margination; Migration.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300037

Morphological Characterization of the Leukocytes in Circulating Blood of the Turtle (Phrynops hilarii)
Provedor de dados: International Journal of Morphology
Autores: Pitol,Dimitrius Leonardo; Issa,Joao Paulo Mardegan; Caetano,Flávio Henrique; Lunardi,Laurelúcia Orive.
The Phrynops hilarii specie of turtle has its characterization not well defined in the literature, it was proposed in this study the leukocyte characterization of the blood, stained by Leishman and analyzed under light and transmission electron microscope. It was not observe any cellular type with similar characteristics to neutrophils in mammalian group. We believed, based on the data obtained in this study that the heterophils have a morphofuncional analogy with another neutrophils belonged to mammalian group. This conclusion is being supported in many recent studies found in the literature
Tipo: Journal article
Palavras-chave: Turtle; Morphology; Leukocytes; Blood.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400002

A C-type lectin from Bothrops jararacussu venom can adhere to extracellular matrix proteins and induce the rolling of leukocytes
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Elífio-Esposito,S. L.; Hess,P. L.; Moreno,A. N.; Lopes-Ferreira,M.; Ricart,C. A. O.; Souza,M. V.; Hasselman-Zielinski,F.; Becker,J. A.; Pereira,L. F.
Purification of a lectin from Bothrops jararacussu venom (BjcuL) was carried out using agarose-D-galactose affinity gel. MALDI-TOF gave a major signal at m/z 32028, suggesting the presence of a dimmer composed of two identical subunits. Divalent cations were required for the lectin activity, as complete absence of such ions reduced hemagglutination. BjcuL was more effective at neutral pH and showed total loss of activity at pH values below 4.0 and above 9.0. Its agglutinating activity remained stable at 25°C until 60min, but increased when at 35°C for at least 15min. Adhesion assays to extracellular matrix (ECM) glycoproteins showed that the biotinylated lectin (0.039-5.0µg/100µl) was capable of binding to fibronectin and vitronectin in a dose-dependent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venoms; Fibronectins; Vitronectin; Snakes; Intravital microscopy; Leukocytes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400009

Weight-length relationship, condition factor and blood parameters of farmed Cichla temensis Humboldt, 1821 (Cichlidae) in central Amazon
Provedor de dados: Neotropical Ichthyology
Autores: Tavares-Dias,Marcos; Monteiro,Adriana Maria de Castro; Affonso,Elizabeth Gusmão; Amaral,Kárlia Dalla Santa.
Farming of native carnivore fish species has drawn attention due to their promising use in aquaculture. Among these species, tucunaré of the genus Cichla stand out, them being of high economical interest for sport fishing and Amazon's industry of ornamental fish. The present study describes the weight-length relationship (WLR), relative condition factor (Kn), red blood cell parameters, thrombocytes and leukocytes count of Cichla temensis Humboldt, 1821, farmed in central Amazon. Fish that underwent food training during fingerling culture received extruded ration containing 45% of crude protein during fattening, and had Kn with values from 0.925-1.199, which indicated good health condition during the culture. The equation obtained from the WLR was W =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culture; Freshwater fish; Hematology; Leukocytes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100009

Hematological profile of beef cattle with divergent residual feed intake, following feed deprivation
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Gomes,Rodrigo da Costa; Siqueira,Renata Farinelli de; Ballou,Michael Alan; Stella,Tiago Roberto; Leme,Paulo Roberto.
The objective of this work was to characterize the hematological profiles of steers and bulls, according to residual feed intake (RFI), after feed deprivation. Twenty‑month‑old Nellore steers and bulls were fed feedlot diets for 70 and 56 days, respectively. RFI was calculated as the difference between actual feed intake and expected feed intake. More and less efficient steers and bulls, according to RFI, were subjected to 24 hours of food deprivation. Blood was sampled prior to and following the withdrawal period. Hematological analyses included total and differential white blood cell count, red blood cell count and morphology, and plasma glucose concentration. Variation in RFI did not influence plasma glucose concentration or blood cell profiles. Glucose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos indicus; Fasting; Feed efficiency; Leukocytes; Stress.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900019

Substitution of blood plasma with soy protein concentrate in piglet diet
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Resende,Moisés Queiroz; Mascarenhas,Alessandra Gimenez; Mello,Heloisa Helena de Carvalho; Arnhold,Emmanuel; Teixeira,Karla Andrade; Rufino,Luciana Moura; Nunes,Romão da Cunha.
ABSTRACT In this study, we evaluated the effects of substitution of partial and total spray-dried blood plasma (BP) with soy protein concentrate (SPC) in the diet of post-weaning piglets on their performance, intestinal histomorphometry, and blood parameters. Fifty-four castrated male piglets, weaned at an average age of 21 days and an initial average weight of 7.01±0.294 kg, were distributed in a completely randomized block design, with three diets (0%SPC-5%BP, 2.5%SPC-2.5%BP, and 5%SPC-0%BP), six replicates, and three piglets per experimental unit. The experimental feeds were formulated to meet the requirements of the piglets at 21-32 and 33-42 days of age (pre-starter diet) and 43-66 days of age (starter diet). From 21 to 32 and 21 to 42 days of age,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feed; Performance; Leukocytes; Swine; Villus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000400324

Características hematológicas do tambaqui Colossoma macropomum Cuvier (Osteichthyes, Characidae) em sistema de monocultivo intensivo: II. Leucócitos
Provedor de dados: Zoologia
Autores: Tavares-Dias,Marcos; Sandrim,Elziane F. Silva; Campos-Filho,Eugênio de.
The leukocytes parameters in approximately one-year-old freshwater fish Colossoma macropomum Cuvier, 1818 (tambaqui) kept in an intensive monobreeding system as well as the correlation among these parameters and the biometric data (total weight and standard length) were investigated. The mean value of the white blood cell count (WBC) in the peripheral blood of tambaqui was 2663.3±1288µl and in the differential count, the following means were observed: neutrophils (1566.2±754µl); lymphocytes (973.6±447µl); monocytes (86.7±123µl) and special granulocitic cells (7.8±144µl). The blood parameters studied were positively correlated among one another, but were negatively correlated with the standard length. However, no correlation was with the weigth of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characidae; Colossoma macropomum; Freshwater fish; Leukocytes; Tambaqui.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000100012

Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion
Provedor de dados: Acta Amazonica
Autores: Tavares-Dias,Marcos; Oliveira-Júnior,Antônio Aderson; Marcon,Jaydione Luiz.
The aim of this paper is to compare three different methods for counting white blood cells [WBC] (Natt and Herrick method, estimation with 1,000 and 2,000 erythrocytes) and three methods for counting total thrombocytes [TT] (Wojtaszek method, estimation with 1,000 and 2,000 erythrocytes) in a South American freshwater turtle species, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae). Direct WBC counts using the Natt and Herrick method showed limitations, which are discussed here. The WBC and TT counts using 1,000 erythrocytes from blood smears are not recommended for Amazon turtles nor other reptilian species, since wide variation in counts can be observed. Estimation methods for determining WBC and TT based on 2,000 erythrocytes of blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon turtles; Blood; Leukocytes; Podocnemis expansa; Thrombocytes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000200020

Hematological and biochemical responses of pirarucu (Arapaima gigas, Arapaimidae) fed with diets containing a glucomannan product derived from yeast and algae
Provedor de dados: Acta Amazonica
Autores: HOSHINO,Maria Danielle Figueiredo Guimarães; MARINHO,Renata das Graças Barbosa; PEREIRA,Diogle Farias; YOSHIOKA,Eliane Tie Oba; TAVARES-DIAS,Marcos; OZORIO,Rodrigo Otavio de Almeida; RODRIGUEZ,Anselmo Fortunato Ruiz; RIBEIRO,Ricardo Amaral; FARIA,Fernando Sérgio Escócio Drummond Viana de.
ABSTRACT The hematological and biochemical responses of pirarucu fingerlings (Arapaima gigas) fed with diets containing different concentrations of a glucomannan product derived from yeast and algae were evaluated in order to ascertain the effect of these diets on fish physiology. Four treatments were conducted, with three replications, with 12 fish in each tank. The product evaluated (MycosorbA+(r)) was incorporated into the commercial diet, at four concentrations: 0, 1, 2 and 4 g.kg-1, called M0%, M0.1%, M0.2% and M0.4%, respectively. After 45 days of feeding, blood samples from six fish in each replicate were collected to perform the analyses. Their weight and length were determined to calculate the condition factor and weight gain, but no differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorella vulgaris; Saccharomyces cerevisiae; Leukocytes; Albumin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000200087

Comparative study on hematological parameters of farmed matrinxã, Brycon amazonicus Spix and Agassiz, 1829 (Characidae: Bryconinae) with others Bryconinae species
Provedor de dados: Acta Amazonica
Autores: Tavares-Dias,Marcos; Affonso,Elizabeth Gusmão; Oliveira,Sarah Ragonha; Marcon,Jaydione Luiz; Egami,Mizue Imoto.
The aim of this study was not only to determine the red blood cells parameters, thrombocyte and leukocyte counts in farmed Brycon amazonicus (matrinxã), to compare these parameters among Bryconinae species from literature, and also to investigate the presence of special granulocytic cells in these fish. The results of the blood cells parameters here established for farmed B. amazonicus, a species of great economic importance in Brazilian aquaculture, could help a better understanding of the blood features in natural populations of this Amazon species. Blood parameters varied between Bryconinae species investigated, mainly the red blood cell counts, hemoglobin, hematocrit and mean corpuscular volume (MCV). The presence of the blood granulocytes, neutrophils...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukocytes; Freshwater fish; Blood; Amazon; Brycon amazonicus; Bryconinae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000400026

Haematological assessment of four amazonian ornamental armoured catfish (Teleostei, Loricariidae)
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Neves, Mikaelle de Souza; Barbas, Luis André Luz; Couto, Márcia Valeria Silva do; Lopes, Josiane Nazaré Silva; Tavares-Dias, Marcos; Fujimoto, Rodrigo Yudi.
The aim of this study was to characterize the haematological profile of four Amazonian ornamental freshwater armoured catfish: acari-bola (Peckoltia oligospila - L06), acari-pleco (Cochliodon sp. - L145), acari-canoa (Lasiancistrus saetiger - L323) and acari-assacu (Pseudocanthicus spinosus - L160). The highest blood glucose levels (72.47 ± 28.7 mg dL-1) and erythrocyte counts (0.51 ± 0.2 x 106 cel. μL-1) were recorded for acari-canoa. The acari-bola and acari-pleco presented similar concentrations of total plasma protein (TPP) (7.96 ± 1.8 and 7.93 ± 1.8 g dL-1, respectively) against lower TPP concentrations observed in acari-canoa (4.87 ± 1.5 g dL-1) and acari-assacu (6.55 ± 1.5 g dL-1). The acari-assacu had lower total haemoglobin concentration (5.88 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquicultura; Hematológia de peixes blood profile; Leukocytes; Fish; Pleco. aquicultura.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/39554

Ecotoxicity of natural insecticide based on tobacco plant extract and hematological effects on the Nile tilapia (Oreochromis niloticus).
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Millan, Debora Cristina; Centro Universitário Central Paulista; Shiogiri, Natália Sayuri; Universidade Federal de São Carlos; Souza, Naiara Elisabete da Silva de; Universidade Federal de São Carlos; Silva, Hugo Ribeiro da; Universidade Federal de São Carlos; Fernandes, Marisa Narciso; Universidade Federal de São Carlos.
Natural insecticides derived from plant extracts have been used as an alternative to synthetic products in order to reduce environmental contamination. The present study aimed to examine the effects of Fumydro®, a natural insecticide based in the tobacco plant Nicotiana tabacum, on the Nile tilapia (Oreochromis niloticus) by determining the 48-h LC50 and evaluating their effects on hematological variables. Adult specimens of O. niloticus were exposed to four Fumydro® concentrations (200, 300, 400 and 500 μL L-1). The 48-h LC50 of Fumydro® was determined as 370 ± 50 μL L-1. Surviving fish showed increasing in the red blood cells, hemoglobin concentration, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration. The thrombocytes did not...
Palavras-chave: Ecologia toxicity; Erythrocytes; Leukocytes; Teleost; Thrombocytes 2.05.03.00-8.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14131

Evaluation of genotoxic and cytotoxic effects of hydroalcoholic extract of Euphorbia tirucalli (Euphorbiaceae) in cell cultures of human leukocytes
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Machado,Michel M.; Oliveira,Luis F.S. de; Zuravski,Luisa; Souza,Raul O. de; Fischer,Paula; Duarte,Jonathaline A.; Rocha,Manoelly O.; Güez,Camila M.; Boligon,Aline A.; Athayde,Margareth L.
ABSTRACT Euphorbia tirucalli (L.), commonly known as aveloz, has been indiscriminately used in popular medicine to treat various illnesses. However, some components can have devastating consequences. Injury to a cell's genetic material can cause mutations, cancer, and cell death. Our main goal in this work was to evaluate the genotoxic and cytotoxic effects of E. tirucalli extract on human leukocytes. For this purpose, we performed a phytochemical analysis to evaluate the plant's components. In the second step, we treated cultured human leukocytes with different concentrations of the dry extract of the plant and then evaluated the oxidative and genotoxic profiles of these leukocytes. We found that at 1% and 10% concentrations, the aveloz extract acted as a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aveloz; Euphorbia tirucalli; Leukocytes; Genotoxic; Cytotoxic.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100017

Relationship between periodontal outcomes and serum biomarkers changes after non-surgical periodontal therapy
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: LOBÃO,WALDER J.M.; CARVALHO,ROSANA C.C. DE; LEITE,SANDRA A.M.; RODRIGUES,VANDILSON P.; BATISTA,JOSÉ EDUARDO; GOMES-FILHO,ISAAC S.; PEREIRA,ANTONIO L.A.
Abstract: The systemic effect of chronic periodontitis (CP) has been suggested by several studies as an etiologic factor and modulator of diseases based on the changes in the inflammatory marker levels. This study aimed to investigate the relationship between the changes in clinical periodontal outcomes and serum biomarkers (CRP, IL-6, albumin and percentage of leukocytes) after non-surgical periodontal therapy in systemically healthy adults. An interventional study was conducted with a sample of 29 individuals without CP (control group) and 33 with CP (CP group). Periodontal clinical variables were recorded, and the serum levels of inflammatory markers were measured. Statistical analysis included the chi-square and Student’s t-tests and Pearson’s...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic periodontitis; Inflammation mediators; Interleukin-6; C-reactive protein; Leukocytes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300805

Hematological and biometric traits of tuvira Gymnotus inaequilabiatus (Valenciennes, 1839) (Gymnotiformes: Gymnotidae) from the Brazilian Pantanal
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: RODRIGUES,ROBSON A.; SILVA,EDUARDA S.; MARCONDES,SANDRIELY F.; GALINDO,GIZELA M.; OLIVEIRA,GUSTAVO G. DE; SOUZA,ALDA I. DE; RAGUSA-NETTO,JOSÉ; FERNANDES,CARLOS E.
ABSTRACT This study describes the hematological and biometric characteristics of male and female Gymnotus species from the Pantanal, Mato Grosso do Sul state, Brazil. Fifty adult specimens of Gymnotus inaequilabiatus were weighed, measured, and then euthanized. Blood was collected by puncturing the celiac mesenteric vein to determine the hematocrit, hemoglobin content, number of erythrocytes, mean corpuscular volume, mean corpuscular hemoglobin concentration, glucose level, absolute value of leukocytes, and relative value of leukocytes and thrombocytes. Body weight and relative condition factor did not differ (P &gt; 0.05) between the sexes, as well as erythrogram and the blood glucose values. Hematocrit ranged from 18.0% to 54.0%; hemoglobin from 1.1 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood glucose; Erythrogram; Leukocytes; Sex differences; Thrombocytes.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100049

Composto homeopático reduz a liberação de ânion superóxido pelas células mononucleares de ema (Rhea americana)
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Bertoldo,W.R; França,J.L; Fernandes,L.T.O; França-Botelho,A.C.
The action of the "Stress Factor Ostrich (Arenales - Fauna and Flora)" was tested in the release of superoxide anion by cells in the peripheral blood of rhea (Rhea americana). Sixteen samples of 0.5mL of venous blood were collected through the jugular vein in the morning and placed in heparinized tubes. The leukocytes were separated into polymorphonuclear (PMN) and mononuclear (MN). The production of superoxide anion by phagocytes of peripheral blood was determined using the chromogen ferricytochrome C. There was a reduction of superoxide by MN cells in the presence of "Stress Factor Ostrich" indicating a positive influence of product against oxidative stress. Furthermore, future researches, such as the evaluation of other reactive oxygen intermediates and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ema; Estresse; Superóxido; Leucócitos; Rhea americana; Stress; Superoxide; Leukocytes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500027

Hepatocytes, rather than leukocytes reverse DNA damage in vivo induced by whole body y-irradiation of mice, as shown by the alkaline comet assay
Provedor de dados: Biological Research
Autores: PINCHEIRA,JUANA; CARRERA,PILAR; MARCELAIN,KATHERINE; DE LA TORRE,CONSUELO.
DNA damage repair was assessed in quiescent (G0) leukocytes and in hepatocytes of mice, after 1 and 2 hours recovery from a single whole body y-irradiation with 0.5, 1 or 2 Gy. Evaluation of single-strand breaks (SSB) and alkali-labile sites together were carried out by a single-cell electrophoresis at pH&gt;13.0 (alkaline comet assay). In non-irradiated (control) mice, the constitutive, endogenous DNA damage (basal) was around 1.5 times higher in leukocytes than in hepatocytes. Irradiation immediately increased SSB frequency in both cell types, in a dose-dependent manner. Two sequential phases took place during the in vivo repair of the radio-induced DNA lesions. The earliest one, present in both hepatocytes and leukocytes, further increased the SSB...
Tipo: Journal article
Palavras-chave: Alkaline comet assay; Ionizing radiation; Hepatocytes; Leukocytes; In vivo DNA repair.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200011

Use of Inert Gases for the Preservation of Nuclear Blood Cells
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Khudyakov,Andrey Nikolaevich; Polezhaeva,Tatyana Vitalyevna; Zaitseva,Oksana Olegovna; Sergushkina,Marta Igorevna; Solomina,Olga Nurzadinovna.
Abstract The subject of the study was the stability of human white blood cell membranes subject to noble gases (xenon ad krypton, 0.6 mPa) clathrate cryoanabiosis (‒80°C). A unique portable stainless steel low pressure container with a compartment for flexible plastic container was designed to ensure that the cells are saturated with gases. The samples were warmed after 1 and 30 days in a water bath (+38°C) for 35-50 sec, while the container was being tilted (2-3 times per second), until the temperature of the biological object reached +3±1°C. It was demonstrated that after 30 days of clathrate anabiosis (-80°C) over 95% (of the original number) of leukocytes remain viable, and cell membranes of 54.5±3.4% of them is resistant to trypan blue; granulocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clathrates; Cryopreservation; Xenon; Krypton; Leukocytes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100404

Immunomodulation of Juvenile Pacu, Piaractus mesopotamicus, by Different β(1-3)(1-6)-D glucan Products
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Sabioni,Rafael Estevan; Zanuzzo,Fábio Sabbadin; Cyrino,José Eurico Possebon.
Abstract Stress in intensive fish farming hamper immune function of fish and cause losses by disease outbreaks, a situation that can be minimized, but cannot be completely circumvented, by the use of immunomodulators. Addition of immunomodulators to aquafeeds has thus become a common practice. β-glucan (BG) is one of most studied and effective immunomodulators, aquaculture purposes included. Extracted from cell walls of bacteria, fungi and selected cereals, BG activity depends on the source and extraction methods. This study evaluated effects of two BG products (BG1 and BG2), extracted from Saccharomyces cerevisiae under varying extraction methods and with different immune activity, on the feeding of pacu Piaractus mesopotamicus juveniles. BG1 provided...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunostimulant; Aquaculture; Stress; Aeromonas hydrophila; Leukocytes; Lysozyme.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100410

Hank’s balanced salt solution: an alternative resuspension medium to label autologous leukocytes. Experience in inflammatory bowel disease
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Martin-Comin,Joseph; Cardoso,Valbert Nascimento; Plaza,Pedro; Roca,Manoel.
In this work Hank's balanced salt solution (HBSS) has been used, as resuspension medium, instead of leukocyte poor plasma (LPP) to label autologous white blood cells in 28 patients with suspicion af active inflammatory bowel disease.Labelled cells were reinjected and anterior and caudo-craneal views were obtained at 30 min, 2 h and 6 h p.i. Regions of interest were outlined on liver, spleen, lung, bone marrow (spine), background and lesions and the organ/background activity ratios were calculated in all scans. Patients were classified into 2 groups: Group 1: LPP, 30 patients and Groups 2: HBSS, 28 patients. Labelling efficiency was higher in HBBS group (89.0 ± 3.2 %) than in the LPP group (6.5 ± 6.3%). Organ/background activity ratios were similar in both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 99mTechnetium; Leukocytes; Hank' s solution; Inflammatory.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500006

Polyomaviruses BK and JC DNA infection in peripheral blood cells from blood donors
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Haghighi,Malihe Fathi; Seyyedi,Noorossadat; Farhadi,Ali; Zare,Farahnaz; Kasraian,Leila; Dehbidi,Gholam Reza Refiei; Ranjbaran,Reza; Behzad-Behbahani,Abbas.
ABSTRACT  Objectives: To investigate the prevalence of human polyomavirus (BK and JC viruses) infection in peripheral blood mononuclear cells of healthy blood donors.  Methods: The study included 250 healthy blood donors. Five-milliliter blood was drawn into sterile EDTA tubes and PBMCs were isolated from whole blood. The isolated PBMCs were counted and stored at −70 °C for future investigation. DNA was extracted and subjected to simple, sensitive and specific semi-nested PCR as well as QPCR using both general and specific primers for different assays.  Results: Of 250 blood samples, 66 (26.4%) were positive for BKV DNA (146-34,514 copies/106 cells). JC DNA was found in 45 (18%) blood samples (65-21,250 copies/106 cells). Co-infection with these viruses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood donors; Leukocytes; Mononuclear; Polyomavirus; Real-time polymerase chain reaction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100022

Arachidonic acid triggers an oxidative burst in leukocytes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pompeia,C.; Cury-Boaventura,M.F.; Curi,R.
The change in cellular reducing potential, most likely reflecting an oxidative burst, was investigated in arachidonic acid- (AA) stimulated leukocytes. The cells studied included the human leukemia cell lines HL-60 (undifferentiated and differentiated into macrophage-like and polymorphonuclear-like cells), Jurkat and Raji, and thymocytes and macrophages from rat primary cultures. The oxidative burst was assessed by nitroblue tetrazolium reduction. AA increased the oxidative burst until an optimum AA concentration was reached and the burst decreased thereafter. In the leukemia cell lines, optimum concentration ranged from 200 to 400 µM (up to 16-fold), whereas in rat cells it varied from 10 to 20 µM. Initial rates of superoxide generation were high,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachidonic acid; NADPH oxidase; Nitroblue tetrazolium; Leukocytes; Reactive oxygen species.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100013

Effect of fatty acids on leukocyte function
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pompéia,C.; Lopes,L.R.; Miyasaka,C.K.; Procópio,J.; Sannomiya,P.; Curi,R.
Fatty acids have various effects on immune and inflammatory responses, acting as intracellular and intercellular mediators. Polyunsaturated fatty acids (PUFAs) of the omega-3 family have overall suppressive effects, inhibiting lymphocyte proliferation, antibody and cytokine production, adhesion molecule expression, natural killer cell activity and triggering cell death. The omega-6 PUFAs have both inhibitory and stimulatory effects. The most studied of these is arachidonic acid that can be oxidized to eicosanoids, such as prostaglandins, leukotrienes and thromboxanes, all of which are potent mediators of inflammation. Nevertheless, it has been found that many of the effects of PUFA on immune and inflammatory responses are not dependent on eicosanoid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty acids; Immune system; Inflammation; Dietary lipids; Leukocytes.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100001

Structure and function of the selectin ligand PSGL-1
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cummings,R.D.
P-selectin glycoprotein ligand-1 (PSGL-1) is a dimeric mucin-like 120-kDa glycoprotein on leukocyte surfaces that binds to P- and L-selectin and promotes cell adhesion in the inflammatory response. The extreme amino terminal extracellular domain of PSGL-1 is critical for these interactions, based on site-directed mutagenesis, blocking monoclonal antibodies, and biochemical analyses. The current hypothesis is that for high affinity interactions with P-selectin, PSGL-1 must contain O-glycans with a core-2 branched motif containing the sialyl Lewis x antigen (NeuAc&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;2&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;3Galß1&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;4[Fuc&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;1&lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P-selectin; L-selectin; E-selectin; PSGL-1; O-glycosylation; Glycoprotein; Mucin; Tyrosine sulfate; Cell adhesion; Leukocytes; Neutrophils.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500004

Indole ring oxidation by activated leukocytes prevents the production of hypochlorous acid
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ximenes,V.F.; Paino,I.M.M.; Faria-Oliveira,O.M.M. de; Fonseca,L.M. da; Brunetti,I.L.
Hypochlorous acid (HOCl) released by activated leukocytes has been implicated in the tissue damage that characterizes chronic inflammatory diseases. In this investigation, 14 indole derivatives, including metabolites such as melatonin, tryptophan and indole-3-acetic acid, were screened for their ability to inhibit the generation of this endogenous oxidant by stimulated leukocytes. The release of HOCl was measured by the production of taurine-chloramine when the leukocytes (2 x 10(6) cells/mL) were incubated at 37ºC in 10 mM phosphate-buffered saline, pH 7.4, for 30 min with 5 mM taurine and stimulated with 100 nM phorbol-12-myristate acetate. Irrespective of the group substituted in the indole ring, all the compounds tested including indole,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukocytes; Myeloperoxidase; Hypochlorous acid; Indole ring; Melatonin; Indole-3-acetic acid.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100003

Contribution of CD4+ T cells to the early mechanisms of ischemia- reperfusion injury in a mouse model of acute renal failure
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pinheiro,H.S.; Camara,N.O.S.; Noronha,I.L.; Maugeri,I.L.; Franco,M.F.; Medina,J.O.A.P.; Pacheco-Silva,A.
Renal ischemia-reperfusion (IR) injury is the major cause of acute renal failure in native and transplanted kidneys. Mononuclear leukocytes have been reported in renal tissue as part of the innate and adaptive responses triggered by IR. We investigated the participation of CD4+ T lymphocytes in the pathogenesis of renal IR injury. Male mice (C57BL/6, 8 to 12 weeks old) were submitted to 45 min of ischemia by renal pedicle clamping followed by reperfusion. We evaluated the role of CD4+ T cells using a monoclonal depleting antibody against CD4 (GK1.5, 50 µ, ip), and class II-major histocompatibility complex molecule knockout mice. Both CD4-depleted groups showed a marked improvement in renal function compared to the ischemic group, despite the fact that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemia-reperfusion; Kidney inflammation; Leukocytes; CD4+ T lymphocytes; Kidney transplantation; Acute renal failure.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400015

Disintegrins: integrin selective ligands which activate integrin-coupled signaling and modulate leukocyte functions
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Barja-Fidalgo,C.; Coelho,A.L.J.; Saldanha-Gama,R.; Helal-Neto,E.; Mariano-Oliveira,A.; Freitas,M.S. de.
Extracellular matrix proteins and cell adhesion receptors (integrins) play essential roles in the regulation of cell adhesion and migration. Interactions of integrins with the extracellular matrix proteins lead to phosphorylation of several intracellular proteins such as focal adhesion kinase, activating different signaling pathways responsible for the regulation of a variety of cell functions, including cytoskeleton mobilization. Once leukocytes are guided to sites of infection, inflammation, or antigen presentation, integrins can participate in the initiation, maintenance, or termination of the immune and inflammatory responses. The modulation of neutrophil activation through integrin-mediated pathways is important in the homeostatic control of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Integrin; Disintegrin; Leukocytes; Activation; Proliferation; Signal transduction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000008

Expression of the chemokine receptor CXCR4 on lymphocytes of leprosy patients
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Mendonça,V.A.; Alvim de Melo,G.E.B.; Araújo,M.G.; Borges,V.O.; Costa,R.D.; Martins-Filho,O.A.; Teixeira- Carvalho,A.; Sathler-Avelar,R.; Teixeira,M.M.; Teixeira,A.L.
Leprosy is caused by Mycobacterium leprae, which induces chronic granulomatous infection of the skin and peripheral nerves. The disease ranges from the tuberculoid to the lepromatous forms, depending on the cellular immune response of the host. Chemokines are thought to be involved in the immunopathogenesis of leprosy, but few studies have investigated the expression of chemokine receptors on leukocytes of leprosy patients. In the present study, we evaluated 21 leprosy patients (M/F: 16/5) with a new diagnosis from the Dermatology Outpatient Clinic of the University Hospital, Federal University of Minas Gerais. The control group was composed of 20 healthy members (M/F: 15/5) of the community recruited by means of announcements. The expression of CCR2,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leprosy; Chemokine receptors; CXCR4; Leukocytes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200009

WBC count and functional changes induced by co-administration of clofazimine and clarithromycin, in single and multiple doses, in Wistar rats
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Paina,Flávia Aparecida; Miranda,Julise Cunha; Tavares,Cristiane Fernandes de Freitas; Queiroz,Regina Helena da Costa; Souza,Ana Maria de.
Clofazimine and clarithromycin are used to treat leprosy and infections caused by Mycobacterium avium complex. Little data on the toxicity of co-administration of these two drugs are available. Here we evaluated the potential adverse effects of polytherapy with these two drugs in male Wistar rats by determining WBCs counts and other blood cell counts, neutrophilic phagocytosis, and burst oxidative, by flow cytometry. We observed an increase in WBCs, in multiple-dose regimens, and in polymorphonuclear cells, in both single- clarithromycin only and multiple dose regimens. We also observed a reduction in mononuclear cell counts in single and multiple doses. The drugs seem to reverse the mononuclear and polymorphonuclear cell ratio. An increase in oxidative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clofazimine/adverse effects; Clofazimine/experimental study; Clarithromycin/adverse effects; Clarithromycin/experimental study; Leprosy/treatment; Mycobacterium avium/infections; Mycobacterium avium/treatment; Leukocytes; Phagocytosis; Oxidative burst.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300008