Consulta: subjectFacets:"Lipopolysaccharide"
Registros recuperados: 25
Data/hora: 09/06/2024 03:46:10

NMR Structure of rALF-Pm3, an Anti-Lipopolysaccharide Factor from Shrimp: Model of the Possible Lipid A-Binding Site
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Yang, Yinshan; Boze, Helene; Chemardin, Patrick; Padilla, Andre; Moulin, Guy; Tassanakajon, Anchalee; Pugniere, Martine; Roquet, Francoise; Destoumieux Garzon, Delphine; Gueguen, Yannick; Bachere, Evelyne; Aumelas, Andre.
The anti-lipopolysaccharide factor ALF-Pm3 is a 98-residue protein identified in hemocytes from the black tiger shrimp Penaeus monodon. It was expressed in Pichia pastoris from the constitutive glyceraldehyde-3-phosphate dehydrogenase promoter as a folded and N-15 uniformly labeled rALF-Pm3 protein. Its 3D structure was established by NMR and consists of three alpha-helices packed against a four-stranded beta-sheet. The C-34-C-55 disulfide bond was shown to be essential for the structure stability. By using surface plasmon resonance, we demonstrated that rALF-Pm3 binds to LPS, lipid A and to OM(R)-174, a soluble analogue of lipid A. Biophysical studies of rALF-Pm3/LPS and rALF-Pm3/OM(R)-174 complexes indicated rather high molecular sized aggregates, which...
Tipo: Text
Palavras-chave: Ultracentrifugation; Surface plasmon resonance; Septic shock; Structure; NMR; Lipid A; Lipopolysaccharide; Anti lipopolysaccharide factor.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6320.pdf

Resistance to Antimicrobial Peptides in Vibrios
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Destoumieux-garzón, Delphine; Duperthuy, Marylise; Vanhove, Audrey; Schmitt, Paulina; Wai, Sun.
Vibrios are associated with a broad diversity of hosts that produce antimicrobial peptides (AMPs) as part of their defense against microbial infections. In particular, vibrios colonize epithelia, which function as protective barriers and express AMPs as a first line of chemical defense against pathogens. Recent studies have shown they can also colonize phagocytes, key components of the animal immune system. Phagocytes infiltrate infected tissues and use AMPs to kill the phagocytosed microorganisms intracellularly, or deliver their antimicrobial content extracellularly to circumvent tissue infection. We review here the mechanisms by which vibrios have evolved the capacity to evade or resist the potent antimicrobial defenses of the immune cells or tissues...
Tipo: Text
Palavras-chave: Vibrio; Lipopolysaccharide; Outer membrane vesicle; Membrane transporter; Innate immunity; Defensin; Cathelicidin; Bactericidal/permeability-increasing protein.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00276/38767/37308.pdf

Distribution and Expression of Visfatin-Positive Cells in the Spleen of Lipopolysaccharide-Stimulated Piglets
Provedor de dados: International Journal of Morphology
Autores: Xiao,Ke; Qiu,Chang-Wei; Chen,Min; Yang,Zhi; Wang,Jing; Peng,Ke-Mei; Song,Hui.
The histological changes in the spleen and the immunohistochemical expression of visfatin in lipopolysaccharide-stimulated piglets are reported to examine the relation between visfatin and inflammation. The results are as follows: (1) After LPS treated, the spleen displayed thicker capsules and trabecula, the thinner periarterial lymphatic sheath, and the more expandable splenic sinusoid, with an increase in the number of splenic nodules, lymphocytes, ellipsoids of the marginal zone, red blood cells and macrophagocytes. (2) Visfatin-positive cells were mainly distributed in the red pulp of the spleen, with less in splenic nodules and periarterial lymphatic sheath. In the LPS-treated group, the signal intensity and quantity of the visfatin-positive cells...
Tipo: Journal article
Palavras-chave: Visfatin; Spleen; Lipopolysaccharide; Piglets.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400052

Comparison of Bacterial Endotoxin Lipopolysaccharide Concentrations in the Blood, Ovarian Follicular Fluid and Uterine Fluid: A Clinical Case of Bovine Metritis
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: MAGATA, Fumie; ISHIDA, Yoshikazu; MIYAMOTO, Akio; FURUOKA, Hidefumi; INOKUMA, Hisashi; SHIMIZU, Takashi; 宮本, 明夫; 古岡, 秀文; 猪熊, 壽; 清水, 隆.
We investigated the concentration of the bacterial endotoxin lipopolysaccharide (LPS) in the blood, ovarian follicular fluid and uterine fluid of a clinical case of bovine metritis. A 2-year-old lactating Holstein cow exhibited continuous fever &gt;39.5°C for more than 2 weeks after normal calving. The cow produced a fetid, watery, red-brown uterine discharge from the vagina and was diagnosed with metritis. The LPS concentrations in plasma and uterine fluid were 0.94 and 6.34 endotoxin units (EU)/ml, respectively. One of seven follicles showed an extremely high level of LPS (12.40 EU/ml) compared to the other follicles (0.62–0.97 EU/ml). These results might suggest the presence of high concentration of LPS in follicles in cows with postpartum metritis.
Palavras-chave: Dairy cow; Follicle; Lipopolysaccharide; Metritis.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4043

Lipopolysaccharide (LPS) Inhibits Steroid Production in Theca Cells of Bovine Follicles In Vitro: Distinct Effect of LPS on Theca Cell Function in Pre- and Post-selection Follicles
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: MAGATA, Fumie; HORIUCHI, Maya; MIYAMOTO, Akio; SHIMIZU, Takashi; 宮本, 明夫; 清水, 隆.
In postpartum dairy cows, lipopolysaccharide (LPS) derived from gram-negative bacteria such as Escherichia coli causes uterine inflammation and leads to ovarian dysfunction. The aim of this study was to determine the effect of LPS on steroid production in bovine theca cells at different stages of follicular development. Theca cells isolated from pre- and post-selection follicles (PRFs, &lt;8.5 mm in diameter, and POFs, &gt;8.5 mm in diameter, respectively) of bovine ovaries were exposed to LPS under luteinizing hormone (LH) conditions, estradiol (E2) conditions or both conditions in vitro. Bovine theca cells expressed the LPS receptor gene complex: Toll-like receptor 4 (TLR4), CD14 and MD2. LPS suppressed progesterone (P4) and androstenedione (A4)...
Palavras-chave: Androstenedione; Dairy cow; Lipopolysaccharide; Progesterone; Theca cells.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3935

Effects of Dietary Supplementation with Tributyrin and Coated Sodium Butyrate on Intestinal Morphology, Disaccharidase Activity and Intramuscular Fat of Lipopolysaccharide-Challenged Broilers
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Xiong,J; Qiu,H; Bi,Y; Zhou,HL; Guo,S; Ding,B.
ABSTRACT The objectives of this study were to investigate the effects of tributyrin (TB) and coated sodium butyrate (CSB) on intestinal morphology, disaccharidase activity and intramuscular fat of broilers challenged with lipopolysaccharide (LPS). A total of 160 1-day-old healthy Cobb broilers were randomly allocated into four groups: (1) control; (2) LPS, in which broilers were fed a basal diet and intraperitoneally injected with 500 μg/kg LPS on days 38, 40 and 42; (3) TB, in which LPS-challenged broilers were fed basal diet supplemented with 500 mg/kg TB; and (4) CSB, in which LPS-challenged broilers were fed basal diet supplemented with 877 mg/kg CSB. Addition of TB and CSB inhibited (p&lt;0.05) the decrease in villus height in the duodenum and ileum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broilers; Coated sodium butyrate; Intestine; Lipopolysaccharide; Tributyrin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400707

Peracute bovine mastitis caused by Klebsiella pneumoniae
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Ribeiro,M.G.; Motta,R.G.; Paes,A.C.; Allendorf,S.D.; Salerno,T.; Siqueira,A.K.; Fernandes,M.C.; Lara,G.H.B.
Relata-se a ocorrência de graves sintomas de mastite hiperaguda em vaca, causada por Klebsiella pneumoniae, na terceira semana de lactação. Descrevem-se aspectos epidemiológicos, sintomas clínicos, procedimentos de diagnóstico microbiológico, conduta terapêutica e medidas de controle.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Milk; Klebsiella pneumoniae; Endotoxin; Lipopolysaccharide.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200031

Propofol pretreatment attenuates lipopolysaccharide-induced acute lung injury in rats by activating the phosphoinositide-3-kinase/Akt pathway
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhao,L.L.; Hu,G.C.; Zhu,S.S.; Li,J.F.; Liu,G.J.
The aim of this study was to investigate the effect of propofol pretreatment on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the role of the phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) pathway in this procedure. Survival was determined 48 h after LPS injection. At 1 h after LPS challenge, the lung wet- to dry-weight ratio was examined, and concentrations of protein, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) were determined using the bicinchoninic acid method or ELISA. Lung injury was assayed via lung histological examination. PI3K and p-Akt expression levels in the lung tissue were determined by Western blotting. Propofol pretreatment prolonged survival, decreased the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lung injury; Propofol; Lipopolysaccharide; PI3K/Akt pathway.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201062

Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gomes,N.E.; Brunialti,M.K.C.; Mendes,M.E.; Freudenberg,M.; Galanos,C.; Salomão,R.
Lipopolysaccharide (LPS) activates neutrophils and monocytes, inducing a wide array of biological activities. LPS rough (R) and smooth (S) forms signal through Toll-like receptor 4 (TLR4), but differ in their requirement for CD14. Since the R-form LPS can interact with TLR4 independent of CD14 and the differential expression of CD14 on neutrophils and monocytes, we used the S-form LPS from Salmonella abortus equi and the R-form LPS from Salmonella minnesota mutants to evaluate LPS-induced activation of human neutrophils and monocytes in whole blood from healthy volunteers. Expression of cell surface receptors and reactive oxygen species (ROS) and nitric oxide (NO) generation were measured by flow cytometry in whole blood monocytes and neutrophils. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipopolysaccharide; Neutrophils; Monocytes; Cell surface receptors; Reactive oxygen species; Nitric oxide.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900007

Galantamine protects against lipopolysaccharide-induced acute lung injury in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Li,G.; Zhou,CL.; Zhou,QS.; Zou,HD.
Lipopolysaccharide (LPS)-induced endotoxemia triggers the secretion of proinflammatory cytokines and can cause acute lung injury (ALI). The high mobility group box 1 (HMGB1) protein plays an important role as a late mediator of sepsis and ALI. Galantamine (GAL) is a central acetylcholinesterase inhibitor that inhibits the expression of HMGB1. This study evaluated the effects of GAL by measuring levels of inflammatory mediators and observing histopathological features associated with LPS-induced ALI. Sixty 8-10 week old male Sprague-Dawley rats (200-240 g) were randomized into three groups as follows: control group, LPS group (7.5 mg/kg LPS), and LPS+GAL group (5 mg/kg GAL before LPS administration). Histopathological examination of lung specimens obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galantamine; Acute lung injury; Lipopolysaccharide; HMGB1.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200601

Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Chen,Yan; Zheng,Yu; Zhou,Zhihong; Wang,Jinjun.
Tubular-interstitial nephritis (TIN) is characterized by tubular cell damage and inflammatory lesions of kidneys. Baicalein (BAI) is a flavonoid compound found in the roots of Scutellaria baicalensis Georgi. The present study was undertaken to explore the anti-inflammatory and anti-oxidative effects of BAI on TIN patients and a lipopolysaccharide (LPS)-induced TIN cell model. The expression levels of interleukin-6 (IL-6), IL-10, and tumor necrosis factor α in serum samples of TIN patients and culture supernatants of renal proximal tubular epithelial cells (RPTECs) were evaluated using enzyme-linked immunosorbent assay. Creatinine clearance was calculated using the Cockcroft-Gault equation. Activities of malondialdehyde, superoxide dismutase, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tubular-interstitial nephritis; Baicalein; Lipopolysaccharide; Anti-inflammatory effects; NF-κB pathway; MAPK pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000606

Collagen XVIII/endostatin expression in experimental endotoxemic acute renal failure
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cichy,M.C.; Rocha,F.G.G.; Tristão,V.R.; Pessoa,E.A.; Cenedeze,M.A.; Nürmberg Junior,R.; Schor,N.; Bellini,M.H.
Acute renal failure (ARF) is a frequent complication of Gram-negative sepsis, with a high risk of mortality. Lipopolysaccharide (LPS)-induced ARF is associated with hemodynamic changes that are strongly influenced by the overproduction of nitric oxide (NO) through the cytokine-mediated up-regulation of inducible NO synthase. LPS-induced reductions in systemic vascular resistance paradoxically culminate in renal vasoconstriction. Collagen XVIII is an important component of the extracellular matrix expressed in basement membranes. Its degradation by matrix metalloproteases, cathepsins and elastases results in the formation of endostatin, claimed to have antiangiogenic activity and to be a prominent vasorelaxing agent. We evaluated the expression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute renal failure; Lipopolysaccharide; Gene expression; Endostatin; CD31.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200005

Total phenolic, flavonoid, alkaloid and iridoid content and preventive effect of Lider-7-tang on lipopolysaccharide-induced acute lung injury in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Erdenechimeg,Ch.; Guiqide,A.; Dejidmaa,B.; Chimedragchaa,Ch.; Purevsuren,S.
Lider-7-tang, a medicine used for the treatment of respiratory diseases especially pneumonia and fever in Mongolian Traditional Medicine, was selected for this phytochemical and pharmacological study. The objectives of the study were to determine total biological active substances and analyze the effects of Lider-7-tang treatment in rats with acute lung injury (ALI). Quantitative determination of the total active constituents (phenolic, flavonoid, iridoid and alkaloid) of the methanol extract of Lider-7-tang was performed using Folin-Ciocalteu reagent, aluminum chloride reagent, Trim-Hill reagent, and Bromocresol green reagent, respectively. A total of fifty 8–10-week-old male Wistar rats (200–240 g) were randomized into three groups: control group,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Traditional medicine; Lider-7-tang; Phenolic; Acute lung injury; Lipopolysaccharide.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200609

Effects of lipopolysaccharide on low- and high-density cultured rabbit vascular smooth muscle cells: differential modulation of nitric oxide release, ERK1/ERK2 MAP kinase activity, protein tyrosine phosphatase activity, and DNA synthesis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Barbosa de Oliveira,L.C.; Rocha Oliveira,C.J.; Fries,D.M.; Stern,A.; Monteiro,H.P.
Previous studies have shown that exogenously generated nitric oxide (NO) inhibits smooth muscle cell proliferation. In the present study, we stimulated rabbit vascular smooth muscle cells (RVSMC) with E. coli lipopolysaccharide (LPS), a known inducer of NO synthase transcription, and established a connection between endogenous NO, phosphorylation/dephosphorylation-mediated signaling pathways, and DNA synthesis. Non-confluent RVSMC were cultured with 0, 5, 10, or 100 ng/ml of the endotoxin. NO release was increased by 86.6% (maximum effect) in low-density cell cultures stimulated with 10 ng/ml LPS as compared to non-stimulated controls. Conversely, LPS (5 to 100 ng/ml) did not lead to enhanced NO production in multilayered (high density) RVSMC. DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Lipopolysaccharide; Protein tyrosine phosphatases; Mitogen-activated protein kinases; DNA synthesis; Cell density.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200006

Inducible nitric oxide synthase and tumor necrosis factor-alpha in delayed gastric emptying and gastrointestinal transit induced by lipopolysaccharide in mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Inada,T.; Hamano,N.; Yamada,M.; Shirane,A.; Shingu,K.
Gastrointestinal motility disturbances during endotoxemia are probably caused by lipopolysaccharide (LPS)-induced factors: candidates include nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-1ß, and interleukin-6. Flow cytometry was used to determine the effects of LPS and these factors on gastric emptying (evaluated indirectly by determining percent gastric retention; %GR) and gastrointestinal transit (GIT) in male BALB/c mice (23-28 g). NO (300 µg/mouse, N = 8) and TNF-alpha (2 µg/mouse, N = 7) increased (P &lt; 0.01) GR and delayed GIT, mimicking the effect of LPS (50 µg/mouse). During early endotoxemia (1.5 h after LPS), inhibition of inducible NO synthase (iNOS) by a selective inhibitor, 1400 W (150 µg/mouse, N = 11), but not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endotoxin; Inducible nitric oxide synthase; Tumor necrosis factor-alpha; Gastric emptying; Gastrointestinal transit; Lipopolysaccharide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100006

Exogenous normal lymph reduces liver injury induced by lipopolysaccharides in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhao,Z.G.; Zhang,L.L.; Niu,C.Y.; Zhang,J.
The liver is one of the target organs damaged by septic shock, wherein the spread of endotoxins begins. This study aimed to investigate the effects of exogenous normal lymph (ENL) on lipopolysaccharide (LPS)-induced liver injury in rats. Male Wistar rats were randomly divided into sham, LPS, and LPS+ENL groups. LPS (15 mg/kg) was administered intravenously via the left jugular vein to the LPS and LPS+ENL groups. At 15 min after the LPS injection, saline or ENL without cell components (5 mL/kg) was administered to the LPS and LPS+ENL groups, respectively, at a rate of 0.5 mL/min. Hepatocellular injury indices and hepatic histomorphology, as well as levels of P-selectin, intercellular adhesion molecule 1 (ICAM-1), myeloperoxidase (MPO), and Na+-K+-ATPase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exogenous normal lymph; Lipopolysaccharide; Liver injury; Intercellular adhesion molecule 1; Myeloperoxidase; Na+-K+-ATPase.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200128

The effect of bacterial lipopolysaccharide on gastric emptying in rats suffering from moderate renal insufficiency
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rigatto,S.Z.P.; Collares,E.F.
The objective of the present study was to evaluate the response of rats suffering from moderate renal insufficiency to bacterial lipopolysaccharide (LPS, or endotoxin). The study involved 48 eight-week-old male SPF Wistar rats (175-220 g) divided into two groups of 24 animals each. One group underwent 5/6 nephrectomy while the other was sham-operated. Two weeks after surgery, the animals were further divided into two subgroups of 12 animals each and were fasted for 20 h but with access to water ad libitum. One nephrectomized and one sham-treated subgroup received E. coli LPS (25 µg/kg, iv) while the other received a sterile, pyrogen-free saline solution. Gastric retention (GR) was determined 10 min after the orogastric infusion of a standard saline test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Lipopolysaccharide; Endotoxin; Renal insufficiency; Rats.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400006

Noncrystalline uric acid inhibits proteoglycan and glycosaminoglycan synthesis in distal tubular epithelial cells (MDCK)
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Borges,F.T.; Dalboni,M.A.; Michelacci,Y.M.; Schor,N.
Hyperuricemia is associated with renal stones, not only consisting of uric acid (UrAc) but also of calcium oxalate (CaOx). Glycosaminoglycans (GAGs) are well-known inhibitors of growth and aggregation of CaOx crystals. We analyzed the effect of noncrystalline UrAc on GAG synthesis in tubular distal cells. MDCK (Madin-Darby canine kidney) cells were exposed to noncrystalline UrAc (80 µg/mL) for 24 h. GAGs were labeled metabolically and characterized by agarose gel electrophoresis. The expression of proteoglycans and cyclooxygenase 2 (COX-2) was assessed by real-time PCR. Necrosis, apoptosis and prostaglandin E2 (PGE2) were determined by acridine orange, HOESCHT 33346, and ELISA, respectively. CaOx crystal endocytosis was evaluated by flow cytometry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Noncrystalline uric acid; Glycosaminoglycan; MDCK; Lipopolysaccharide; Apoptosis; Proteoglycan.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000006

Impairment of the hematological response and interleukin-1β production in protein-energy malnourished mice after endotoxemia with lipopolysaccharide
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Fock,R.A.; Vinolo,M.A.R.; Blatt,S.L.; Borelli,P.
The objectives of this study were to determine if protein-energy malnutrition (PEM) could affect the hematologic response to lipopolysaccharide (LPS), the interleukin-1β (IL-1β) production, leukocyte migration, and blood leukocyte expression of CD11a/CD18. Two-month-old male Swiss mice were submitted to PEM (N = 30) with a low-protein diet (14 days) containing 4% protein, compared to 20% protein in the control group (N = 30). The total cellularity of blood, bone marrow, spleen, and bronchoalveolar lavage evaluated after the LPS stimulus indicated reduced number of total cells in all compartments studied and different kinetics of migration in malnourished animals. The in vitro migration assay showed reduced capacity of migration after the LPS stimulus in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood; Bone marrow; Spleen; Interleukin-1β; Lipopolysaccharide; Malnutrition.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200009

Immune cells and oxidative stress in the endotoxin tolerance mouse model
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Melo,E.S.; Barbeiro,H.V.; Ariga,S.; Goloubkova,T.; Curi,R.; Velasco,I.T.; Vasconcelos,D.; Soriano,F.G.
Sepsis is a systemic inflammatory response that can lead to tissue damage and death. In order to increase our understanding of sepsis, experimental models are needed that produce relevant immune and inflammatory responses during a septic event. We describe a lipopolysaccharide tolerance mouse model to characterize the cellular and molecular alterations of immune cells during sepsis. The model presents a typical lipopolysaccharide tolerance pattern in which tolerance is related to decreased production and secretion of cytokines after a subsequent exposure to a lethal dose of lipopolysaccharide. The initial lipopolysaccharide exposure also altered the expression patterns of cytokines and was followed by an 8- and a 1.5-fold increase in the T helper 1 and 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sepsis; Cytokines; Immune tolerance; Lipopolysaccharide; Murine sepsis model; T helper cells.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100009

Glycyrrhizin attenuates endotoxin- induced acute liver injury after partial hepatectomy in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Tang,B.; Qiao,H.; Meng,F.; Sun,X.
Massive hepatectomy associated with infection induces liver dysfunction, or even multiple organ failure and death. Glycyrrhizin has been shown to exhibit anti-oxidant and anti-inflammatory activities. The aim of the present study was to investigate whether glycyrrhizin could attenuate endotoxin-induced acute liver injury after partial hepatectomy. Male Wistar rats (6 to 8 weeks old, weighing 200-250 g) were randomly assigned to three groups of 24 rats each: sham, saline and glycyrrhizin. Rats were injected intravenously with lipopolysaccharide (LPS) 24 h after 70% hepatectomy. Glycyrrhizin, pre-administered three times with 24 h intervals 48 h before hepatectomy, prolonged the survival of rats submitted to partial hepatectomy and LPS injection, compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycyrrhizin; Lipopolysaccharide; Hepatectomy; Inflammatory cytokine; Apoptosis; Endotoxemia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200007

Lipopolysaccharide-induced dental pulp cell apoptosis and the expression of Bax and Bcl-2 in vitro
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Yang,H.; Zhu,Y.T.; Cheng,R.; Shao,M.Y.; Fu,Z.S.; Cheng,L.; Wang,F.M.; Hu,T.
Lipopolysaccharide exerts many effects on many cell lines, including cytokine secretion, and cell apoptosis and necrosis. We investigated the in vitro effects of lipopolysaccharide on apoptosis of cultured human dental pulp cells and the expression of Bcl-2 and Bax. Dental pulp cells showed morphologies typical of apoptosis after exposure to lipopolysaccharide. Flow cytometry showed that the rate of apoptosis of human dental pulp cells increased with increasing lipopolysaccharide concentration. Compared with controls, lipopolysaccharide promoted pulp cell apoptosis (P &lt; 0.05) from 0.1 to 100 μg/mL but not at 0.01 μg/mL. Cell apoptosis was statistically higher after exposure to lipopolysaccharide for 3 days compared with 1 day, but no difference was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental pulp cells; Lipopolysaccharide; Apoptosis; Bax; Bcl-2.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100003

Effect of bacterial lipopolysaccharide on gastric emptying of liquids in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
The objectives of the present investigation were 1) to study the effect of bacterial lipopolysaccharide (LPS) on rat gastric emptying (GE) and 2) to investigate a possible involvement of the vagus nerve in the gastric action of LPS. Endotoxin from E. coli (strain 055:B5) was administered sc, ip or iv to male Wistar rats (220-280 g body weight) at a maximum dose of 50 µg/kg animal weight. Control animals received an equivalent volume of sterile saline solution. At a given time period after LPS administration, GE was evaluated by measuring gastric retention 10 min after the orogastric infusion of a test meal (2 ml/100 g animal weight), which consisted of 0.9% NaCl plus the marker phenol red (6 mg/dl). One group of animals was subjected to bilateral...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gastric emptying; Lipopolysaccharide; Vagus nerve.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200008

Propofol exerts anti-inflammatory effects in rats with lipopolysaccharide-induced acute lung injury by inhibition of CD14 and TLR4 expression
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ma,Ling; Wu,Xiu-Ying; Zhang,Li-Hong; Chen,Wei-Min; Uchiyama,Akinori; Mashimo,Takashi; Fujino,Yuji.
We investigated the effect of propofol (Prop) administration (10 mg kg-1 h-1, intravenously) on lipopolysaccharide (LPS)-induced acute lung injury and its effect on cluster of differentiation (CD) 14 and Toll-like receptor (TLR) 4 expression in lung tissue of anesthetized, ventilated rats. Twenty-four male Wistar rats were randomly divided into three groups of 8 rats each: control, LPS, and LPS+Prop. Lung injury was assayed via blood gas analysis and lung histology, and tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) levels were determined in bronchoalveolar lavage fluid using ELISA. Real-time polymerase chain reaction was used to detect CD14 and TLR4 mRNA levels, and CD14 and TLR4 protein expression was determined by Western blot. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CD14; Toll-like receptors; Propofol; Lipopolysaccharide; Lung injury.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300299

The reaction of dental pulp to Escherichia coli lipopolysaccharide and Enterococcus faecalis lipoteichoic acid
Provedor de dados: Brazilian Journal of Microbiology
Autores: Oliveira,Laudimar Alves de; Barbosa,Sérgio Valmor.
This research evaluates the effects of the lipopolysaccharides (LPS) from Escherichia coli and lipoteichoic acid (LTA) from Enterococcus faecalis on dental pulp. These molecules are components of the Gram-negative and Gram-positive bacteria cell wall, respectively. Ten dogs were used in the experiment. Inoculation in surgically opened pulp and coronal restoration with glass ionomer was the method chosen. The evaluation times were 1, 7, 15, 30 and 60 days. The results showed that the LPS and LTA, at 150 µg/ml, produced a negative interference in the pulp leading to destruction. LTA caused less damage than LPS.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipopolysaccharide; Endotoxin; Lipoteichoic acid; Dental pulp; Bacteria.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200018