Consulta: subjectFacets:"Liver regeneration"
Registros recuperados: 7
Data/hora: 02/06/2024 07:28:18

Expression analysis on 14-3-3 proteins in regenerative liver following partial hepatectomy
Provedor de dados: 74
Autores: Xue,Deming; Xue,Yang; Niu,Zhipeng; Guo,Xueqiang; Xu,Cunshuan.
Abstract 14-3-3 proteins play a vital part in the regulation of cell cycle and apoptosis as signaling integration points. During liver regeneration, the quiescent hepatocytes go through hypertrophy and proliferation to restore liver weight. Therefore, we speculated that 14-3-3 proteins regulate the progression of liver regeneration. In this study, we analyzed the expression patterns of 14-3-3 proteins during liver regeneration of rat to provide an insight into the regenerative mechanism using western blotting. Only four isoforms (γ, ε, σ and τ/θ) of the 14-3-3 proteins were expressed in regenerative liver after partial hepatectomy (PH). The dual effects, the significant down-regulation of 14-3-3ε and the significant up-regulation of 14-3-3τ/θ at 2 h after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver regeneration; 14-3-3 proteins; Western blotting.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500855

DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy
Provedor de dados: 74
Autores: Li,Deming; Fan,Jinyu; Li,Ziwei; Xu,Cunshuan.
Epidermal growth factor (EGF), a multifunctional growth factor, is a regulator in a wide variety of physiological processes. EGF plays an important role in the regulation of liver regeneration. This study was aimed at investigating the methylation level of EGF gene throughout liver regeneration. DNA of liver tissue from control rats and partial hepatectomy (PH) rats at 10 time points was extracted and a 354 bp fragment including 10 CpG sites from the transcription start was amplified after DNA was modified by sodium bisulfate. The result of sequencing suggested that methylation ratio of four CpG sites was found to be significantly changed when PH group was compared to control group, in particular two of them were extremely striking. mRNA expression of EGF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylation dynamics; Epidermal growth factor; Liver regeneration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300017

Alterations in DNA repair gene expression and their possible regulation in rat-liver regeneration
Provedor de dados: 74
Autores: Wang,Gai-Ping; Xu,Cun-Shuan.
Rapidly proliferating tissue may require enhanced DNA repair capacity in order to avoid fixation of promutagenic DNA lesions to mutations. Partial hepatectomy (PH) triggers cell proliferation during liver regeneration (LR). However, little is known on how DNA repair genes change and how they are regulated at the transcriptional level during LR. In the present study, the Rat Genome 230 2.0 array was used to detect the expression profiles of DNA repair genes during LR, and differential expression of selected genes was confirmed by real-time RT-PCR. 69 DNA repair genes were found to be associated with LR, more than half of which distributed in a cluster characterized by a gradual increase at 24-72h and then returning to normal. The expression of base excision...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Partial hepatectomy; Rat genome array; DNA repair genes; Liver regeneration.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200023

Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver
Provedor de dados: 47
Autores: Martín,Carlos A.; Surur,José Miguel; García,Marcela N.; Corrons,Félix; Badrán,Amado F.
Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 ± 16.1 SE; AgNORs 77.3 ± 3.4 SE) located at 16:00/54 Time of Day / Hours Post- Hepatectomy (TD/HPH), which were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver regeneration; Sinusoid littoral cell; Bromodeoxyuridine; AgNOR; Immunohistochemistry.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100005

The use of light-emitting diodes to stimulate mitochondrial function and liver regeneration of partially hepatectomized rats
Provedor de dados: 56
Autores: Castro-e-Silva,T.; Castro-e-Silva,O.; Kurachi,C.; Ferreira,J.; Zucoloto,S.; Bagnato,V.S.
The biostimulating effect of laser radiation has been observed in many areas of Medicine. However, there are still several questions to be answered, among them the importance of light coherence in the stimulatory process. In the present study, we used light-emitting diodes (LED) to promote the stimulation of liver regeneration after partial hepatectomy in rats. Fourteen male Wistar rats weighing 200-250 g were submitted to partial hepatectomy (70%) followed by LED light irradiation (630 nm) of the remaining part of the liver at two doses, i.e., 10 (N = 7) and 140 (N = 7) J/cm². A group irradiated with laser, 590 nm (N = 7, 15 J/cm²) was performed for the study of proliferating cell nuclear antigen-labeling index. Data are reported as mean ± SEM....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver; Mitochondria; Laser; Light-emitting diode; Liver regeneration; Proliferating cell nuclear antigen.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800006

Effect of hyperbaric oxygenation on the regeneration of the liver after partial hepatectomy in rats
Provedor de dados: 56
Autores: Kurir,T.T.; Markotic,A.; Katalinic,V.; Bozanic,D.; Cikes,V.; Zemunik,T.; Modun,D.; Rincic,J.; Boraska,V.; Bota,B.; Salamunic,I.; Radic,S.
The aim of the present study was to assess the influence of hyperbaric oxygenation (HBO) on rat liver regeneration before and after partial hepatectomy. Rats were sacrificed 54 h after 15% hepatectomy, liver and body weights were measured, and serum alanine transaminase (ALT) and aspartate transaminase (AST) activity and albumin levels were determined. The lipid peroxide level, as indicated by malondialdehyde production in the remnant liver was measured, and liver sections were analyzed by light microscopy. Five groups of 10 rats in each group were studied. The preHBO and pre-hyperbaric pressure (preHB) groups were treated before partial hepatectomy with 100% O2 and 21% O2, respectively, at 202,650 pascals, daily for 3 days (45 min/day). The control group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Partial hepatectomy; Hyperbaric oxygenation; Liver regeneration; Alanine transaminase; Aspartate transaminase.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800014

Angiotensin-converting enzyme inhibition by lisinopril enhances liver regeneration in rats
Provedor de dados: 56
Autores: Ramalho,F.S.; Ramalho,L.N.Z.; Castro-e-Silva Júnior,O.; Zucoloto,S.; Corrêa,F.M.A.
Bradykinin has been reported to act as a growth factor for fibroblasts, mesangial cells and keratinocytes. Recently, we reported that bradykinin augments liver regeneration after partial hepatectomy in rats. Angiotensin-converting enzyme (ACE) is also a powerful bradykinin-degrading enzyme. We have investigated the effect of ACE inhibition by lisinopril on liver regeneration after partial hepatectomy. Adult male Wistar rats underwent 70% partial hepatectomy (PH). The animals received lisinopril at a dose of 1 mg kg body weight-1 day-1, or saline solution, intraperitoneally, for 5 days before hepatectomy, and daily after surgery. Four to six animals from the lisinopril and saline groups were sacrificed at 12, 24, 36, 48, 72, and 120 h after PH. Liver...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Angiotensin-converting enzyme inhibitors; Lisinopril; Bradykinin; Liver regeneration; Cell proliferation; Hepatocyte.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100016