Consulta: subjectFacets:"Low temperature"
Registros recuperados: 9
Data/hora: 11/06/2024 04:13:30

Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA
Provedor de dados: 69
Autores: Vergara,Mauricio; Becerra,Silvana; Díaz-Barrera,Alvaro; Berrios,Julio; Altamirano,Claudia.
We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamster ovary cells in batch and semi-perfusion cultures. In batch cultures using glucose as a carbon source, decreasing the temperature caused a reduction in cell growth and an increase in specific productivity of rh-tPA of 32% at 34ºC and 55% at 31ºC, compared to cultures at 37ºC. Similar behaviour was observed in cultures at 34ºC using galactose as a carbon source. Nonetheless, at 31ºC, the specific productivity of rh-tPA strongly decreased (about 58%) compared to...
Tipo: Journal article
Palavras-chave: CHO cells; Galactose; Glutamate; Low temperature; Perfusion culture; Rh-tPA.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600002

Development of eggs and the planktonic stages of salmon lice (Lepeophtheirus salmonis) at low temperatures
Provedor de dados: 16
Autores: Boxaspen, Karin; Næss, Tore.
To verify if and to what extent egg and nauplii development of the salmon lice take place during winter, the development from egg to the copepodid stage at 2,3,4,5 and 10°C was examined. Newly extruded egg strings from a winter population of salmon lice were individually placed in 6 ml stagnant hatching systems. Initially, no significant differences in egg development time were found between these and larger aerated systems, though a tendency for less synchronised hatching of the total egg string was detected in the stagnant systems (difference&amp;lt; 12 h). In light versus dark conditions the time to hatching was significantly prolonged by darkness (10-15%). The use of small stagnant experimental units was a pronounced simplification of hatching methods...
Tipo: Article / Letter to the editor
Palavras-chave: Salmon lice; Low temperature; Development of early life stages.
Ano: 2000
URL: http://www.repository.naturalis.nl/record/534414

COMPARISON OF RNA EXTRACTION METHODS FOR Passiflora edulis SIMS LEAVES
Provedor de dados: 94
Autores: LUZ,ANNY CAROLYNE DA; PRETTI,IRANY RODRIGUES; BATITUCCI,MARIA DO CARMO PIMENTEL.
ABSTRACT  Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich in secondary metabolites that interfere with RNA extraction primarily by promoting oxidative processes and by precipitating with nucleic acids. This study aimed to analyse three RNA extraction methods, Concert™ Plant RNA Reagent (Invitrogen, Carlsbad, CA, USA), TRIzol® Reagent (Invitrogen) and TRIzol® Reagent (Invitrogen)/ice -commercial products specifically designed to extract RNA, and to determine which method is the most effective for extracting RNA from the leaves of passion fruit plants. In contrast to the RNA extracted using the other 2 methods, the RNA extracted using TRIzol® Reagent (Invitrogen) did not have acceptable A260/A280 and A260/A230...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora edulis; RNA extraction; Concert™; TRIzol®; Low temperature.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000100226

Inheritance of rice cold tolerance at the seedling stage
Provedor de dados: 63
Autores: Cruz,Renata Pereira da; Duarte,Ismael Tiago de Lima; Cabreira,Caroline.
Rice (Oryza sativa L.) cold tolerance at the initial stages of development is a highly desirable trait to be incorporated into the state of Rio Grande do Sul, Brazil, cultivars, but selection for this trait must be performed under controlled temperature conditions, which limits the number of lines that can be evaluated. Knowledge of the inheritance of this trait is important to define breeding strategies. So the aim of this paper was to study the genetic basis of rice cold tolerance at the vegetative stage. Six genotypes with constrasting cold tolerance reactions were crossed in a diallel scheme without the reciprocals. The parents and the F1 and F2 generations were cultivated in a greenhouse until the V4 stage, when they were submitted to 10°C for ten...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa L.; Genetics; Low temperature; Vegetative stage.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000600008

Cold tolerance at the germination stage of rice: methods of evaluation and characterization of genotypes
Provedor de dados: 63
Autores: Cruz,Renata Pereira da; Milach,Sandra Cristina Kothe.
Rice cold tolerance at the germination stage is important in Rio Grande do Sul (RS) where temperatures below 15°C prevent or reduce germination and plant establishment in early sowings. The present study aimed at identifying an adequate method for cold tolerance evaluation of the rice germination stage and at verifying the variability among 24 rice genotypes of different origins. Cold tolerance was evaluated in experiment I, germination under two conditions: 13°C for 28 days and 28°C for seven days, and in experiment II, germination under 28°C for 72 hours, 13°C for 96 hours and again 28°C for 72 hours. In experiment I measured characteristics were germination index, percentage of seeds with coleoptile length superior to 5 mm and percentage of reduction in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa L.; Low temperature; Variability; Indica and Japonica subspecies.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000100001

Simulating the vernalization response of the "Snow Queen" lily (Lilium longiflorum Thunb.)
Provedor de dados: 63
Autores: Streck,Nereu Augusto; Schuh,Mariângela.
Vernalization is a process required by certain plant species, including lilies (Lilium spp.), to enter the reproductive phase, through an exposure to low, non-freezing temperatures. The objective of this study was to evaluate a nonlinear vernalization response function for the "Snow Queen" lily. An experiment was carried out in Santa Maria, RS, Brazil, to provide an independent data set to evaluate the performance of the model. Lily bulbs were vernalized at -0.5, 4.0, and 10ºC during two, four, six, and eight weeks. The daily vernalization rate (fvn) for each treatment was calculated with a beta function, and the effective vernalization days (VD) were calculated by accumulating fvn. The thermal time from plant emergence to visible buds at different VD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low temperature; Flowering; Bulb; Model; Plant development.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200004

Viability of Nezara viridula (L.) eggs for parasitism by Trissolcus basalis (Woll.), under different storage techniques in liquid nitrogen
Provedor de dados: 44
Autores: Corrêa-Ferreira,Beatriz S.; Oliveira,Maria C. N. de.
Different egg conservation techniques were assessed under low temperature conditions to multiply the parasitoid of stink bug eggs Trissolcus basalis (Woll.) (Hymenoptera:Scelionidae). Masses of fresh Nezara viridula (L.) eggs were stored for 12 months in liquid nitrogen (-196ºC), wrapped in aluminum foil, in microcentrifuge tubes ("eppendorf") or in vacuum sealed double plastic bags (500 eggs/technique/month). Every 30 days, 10 egg masses from each of the different storage techniques, were taken out, thawed immediately and subjected to parasitism by T. basalis. Green stink bug eggs, stored at -196ºC presented conservation conditions and viability for T. basalis development, with high parasitism rates and emergence in the eggs stored under the three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insect; Biological control; Stink bug egg conservation; Low temperature; Parasitoid.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000100013

Cotton zoning based on sowing periods of lower risk in Parana state, Brazil
Provedor de dados: 52
Autores: Wrege,Marcos Silveira; Caramori,Paulo Henrique; Gonçalves,Sergio Luiz; Almeida,Wilson Paes de; Marur,Celso Jamil; Pires,José Ricoy; Yamaoka,Ruy Seiji.
Cotton is cultivated in the North and West of Parana State, southern Brazil, under conditions of climatic risk variable in space and time. Risks of temperature below 15ºC at the establishment period, daily average temperature below 20ºC at the stage of cotton boll opening, and soil water deficit for both plant establishment and flowering periods, were estimated to identify homogeneous zones with sowing periods of lower climatic risk. The time interval with adequate temperature, associated with minimum risks of the other factors and yield data from field experiments allowed the identification of seven distinct zones, with best sowing periods ranging from September 20 to November 20. Official credit to the farmers is conditioned by the Central Bank of Brazil...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotton; Climatic risk; Planting dates; Zoning; Low temperature.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100010

Electrical conductivity and mineral composition of the imbibition solution of bean seeds during storage
Provedor de dados: 64
Autores: Silva,Claudia Denise da; Pazeto,Mariana Silva Rosa; Vieira,Roberval Daiton.
The electrical conductivity test has been used to evaluate seed vigour in many species, especially legumes, such as peas, beans and soybeans. The objective of this research was to evaluate the effect of temperature and storage period on the results of the electrical conductivity test and the mineral composition of the imbibition solution of bean seeds (Phaseolus vulgaris L.). Three seed lots of the cultivar IAPAR - 81 and cultivar IPR - TIZIU were stored at 10, 25 and 25-10º C (six months at 10º C and six months at 25º C) The following evaluations were made every three months for a year: seed water content, germination and vigour (accelerated ageing and electrical conductivity) and the potassium, calcium and magnesium contents of the imbibition solution....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris L; Vigour; Leaching; Low temperature.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000200002