Consulta: subjectFacets:"Lymphocytes"
Registros recuperados: 16
Data/hora: 04/06/2024 12:01:01

MLL leukemia-associated rearrangements in peripheral blood lymphocytes from healthy individuals
Provedor de dados: 74
Autores: Brassesco,María Sol; Montaldi,Ana Paula; Gras,Diana Ester; Queiroz,Rosane Gomes de Paula; Martinez-Rossi,Nilce Maria; Tone,Luiz Gonzaga; Sakamoto-Hojo,Elza Tiemi.
Chromosomal translocations are characteristic of hematopoietic neoplasias and can lead to unregulated oncogene expression or the fusion of genes to yield novel functions. In recent years, different lymphoma/leukemia-associated rearrangements have been detected in healthy individuals. In this study, we used inverse PCR to screen peripheral lymphocytes from 100 healthy individuals for the presence of MLL (Mixed Lineage Leukemia) translocations. Forty-nine percent of the probands showed MLL rearrangements. Sequence analysis showed that these rearrangements were specific for MLL translocations that corresponded to t(4;11)(q21;q23) (66%) and t(9;11) (20%). However, RT-PCR failed to detect any expression of t(4;11)(q21;q23) in our population. We suggest that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genomic instability; Lymphocytes; MLL rearrangements.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200005

Acute Effects of Sulphur Mustard Gas on the Number of Lymphocytes in the Rat's Spleen
Provedor de dados: 77
Autores: Mehrdad,Jahanshahi; Bahadoran,H; Asadi,M. H.
Sulphur mustard (SM), commonly known as mustard gas is an alkylating agent that causes serious blisters upon contact with human skin. SM is frequently used as a chemical warfare agent. There is some evidence for sulfur mustard-induced lymph system effects in humans. Between 2000-2001, 42 male albino Wistar rats were used. After accommodation with environment, we divided rats to control, sham and experimental groups (2.5 mg/kg, 5 mg/kg, 10 mg/kg, 20 mg/kg and 40 mg/kg). Then we injected sulphur mustard oil in rat's intraperitoneal space. Then their spleens were removed for histological verification. Our results showed that significant difference in lymphocytes number in experimental groups after 24 hours. The number of lymphocytes in 5, 10, 20 and 40 mg/kg...
Tipo: Journal article
Palavras-chave: Mustard gas; Lymphocytes; Spleen.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000200031

Different Activation of Lymphocytes in Reaction to Babesia microti Infection in iNOS-/- and Wildtype Mice
Provedor de dados: 19
Autores: Remer, Katharina; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行.
Palavras-chave: Lymphocytes; Babesia microti; Spleen; INOS-/- mice; CD4+ cells; CD8+ cells; IFN-γ; Antibody-titer.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/263

In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens
Provedor de dados: 89
Autores: Zimmermann,CEP; Machado,AK; Cadoná,FC; Jaques,JAS; Schlemmer,KB; Lautert,C; Cruz,IBM; Zanette,RA; Leal,DBR; Santurio,JM.
The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p &lt; 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p &lt; 0.01 and p &lt; 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p &lt; 0.001). ROS formation significantly increased at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxin B1; Broilers; Lymphocytes; PicoGreen; Reactive oxygen species.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300012

Active caspase-3 expression levels as bioindicator of individual radiosensitivity
Provedor de dados: 42
Autores: SANTOS,NEYLIANE F.G. DOS; SILVA,RAFAEL F.; PINTO,MARCELA M.P.L.; SILVA,EDVANE B. DA; TASAT,DEBORAH R.; AMARAL,ADEMIR.
ABSTRACT Several molecules and events involved in cell response to radiation-induced damage have been investigated towards a personalized radiotherapy. Considering the importance of active caspase-3 in the proteolytic cascade that ensures radiation-induced apoptosis execution, this research was designed to evaluate the expression levels of this protein as a bioindicator of individual radiosensitivity. Peripheral blood samples of 10 healthy individuals were gamma-irradiated (cobalt-60 source) with 1, 2 and 4 Gy (control: non-irradiated samples), and active caspase-3 expression levels were measured in lymphocytes, by flow cytometry, ex vivo and after different times of in vitro incubation (24, 48 and 72 hours). Short-term incubation of 24 h was the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Active caspase-3; Apoptosis; Flow cytometry; Ionizing radiation; Lymphocytes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200649

Immunology and immunopathology of African trypanosomiasis
Provedor de dados: 42
Autores: Vincendeau,Philippe; Bouteille,Bernard.
Major modifications of immune system have been observed in African trypanosomiasis. These immune reactions do not lead to protection and are also involved in immunopathology disorders. The major surface component (variable surface glycoprotein,VSG) is associated with escape to immune reactions, cytokine network dysfunctions and autoantibody production. Most of our knowledge result from experimental trypanosomiasis. Innate resistance elements have been characterised. In infected mice, VSG preferentially stimulates a Th 1-cell subset. A response of &lt;FONT FACE=Symbol&gt;gd&lt;/FONT&gt; and CD8 T cells to trypanosome antigens was observed in trypanotolerant cattle. An increase in CD5 B cells, responsible for most serum IgM and production of autoantibodies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosome; African trypanosomiasis; Immunology; Macrophage; Lymphocytes; Nitric oxide; Cytokine; Autoantibodies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400004

Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
Provedor de dados: 42
Autores: Esteves,Mabel B.; Marques-Santos,Luis F.; Affonso-Mitidieri,Ottília R.; Rumjanek,Vivian M.
Lymphocytes activated by mitogenic lectins display changes in transmembrane potential, an elevation in the cytoplasmic Ca2+ concentrations, proliferation and/or activation induced cell death. Low concentrations of ouabain (an inhibitor of Na+,K+-ATPase) suppress mitogen-induced proliferation and increases cell death. To understand the mechanisms involved, a number of parameters were analyzed using fluorescent probes and flow cytometry. The addition of 100nM ouabain to cultures of peripheral blood lymphocytes activated with 5µg/ml phytohemagglutinin (PHA) did not modify the increased expression of the Fas receptor or its ligand FasL induced by the mitogen. However, treatment with ouabain potentiated apoptosis induced by an anti-Fas agonist antibody. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocytes; Ouabain; PHA; Apoptosis; Membrane depolarization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000200008

Light microscope observation of circulating human lymphocytes cultured in vitro
Provedor de dados: 52
Autores: Oliveira,Naila Francis Paulo de; Dolder,Mary Anne Heidi; Genari,Selma Candelária.
The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood; In vitro; Lymphocytes; Microscopy; Morphology; Percoll.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013

Chromosome damage in underground coal miners: detection by conventional cytogenetic techniques and by submitting lymphocytes of unexposed individuals to plasma from at-risk groups
Provedor de dados: 54
Autores: Agostini,J.M.S.; Otto,P.A.; Wajntal,A.
Chromosome abnormalities and the mitotic index in lymphocyte cultures and micronuclei in buccal mucosa cells were investigated in a sample of underground mineral coal miners from Southern Brazil. A decreased mitotic index, an excess of micronuclei and a higher frequency of chromosome abnormalities (fragments, polyploidy and overall chromosome alterations) were observed in the miners when compared to age-paired normal controls from the same area. An alternative assay for clastogenesis in occupational exposition was tested by submitting lymphocytes from non-exposed individuals to a pool of plasmas from the exposed population. This assay proved to be very convenient, as the lymphocytes obtained from the same individuals can be used as target as well as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome damage; Underground coal miners; Cytogenetic; Lymphocytes; Plasma; At-risk groups.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400021

Immunophenotype of normal and leukemic bone marrow B-precursors in a Brazilian population. A comparative analysis by quantitative fluorescence cytometry
Provedor de dados: 56
Autores: Rego,E.M.; Garcia,A.B.; Carneiro,J.J.; Falcão,R.P.
The distinction between normal and leukemic bone marrow (BM) B-precursors is essential for the diagnosis and treatment monitoring of acute lymphoblastic leukemia (ALL). In order to evaluate the potential use of quantitative fluorescence cytometry (QFC) for this distinction, we studied 21 normal individuals and 40 patients with CD10+ ALL. We characterized the age-related changes of the CD10, CD19, TdT, CD34 and CD79a densities in normal and leukemic BM. Compared to normal adults, the B-precursors from normal children expressed significantly lower values of CD34-specific antibody binding capacity (SABC) (median value of 86.6 vs 160.2 arbitrary units (a.u.) in children and adults, respectively). No significant age-related difference was observed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lymphoblastic leukemia; Lymphocytes; Quantitative fluorescence cytometry; Flow cytometry; Age-related changes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200005

Cytokine profile and lymphocyte subsets in type 2 diabetes
Provedor de dados: 56
Autores: Francisco,C.O.; Catai,A.M.; Moura-Tonello,S.C.G.; Arruda,L.C.M.; Lopes,S.L.B.; Benze,B.G.; Del Vale,A.M.; Malmegrim,K.C.R.; Leal,A.M.O.
Type 2 diabetes mellitus (T2D) is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the immune system in T2D patients' peripheral blood by quantifying the frequency of lymphocyte subsets and intracellular pro- and anti-inflammatory cytokine production by T cells. Clinical data and blood samples were collected from 22 men (51.6±6.3 years old) with T2D and 20 nonsmoking men (49.4±7.6 years old) who were matched for age and sex as control subjects. Glycated hemoglobin, high-sensitivity C-reactive protein concentrations, and the lipid profile were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; Cytokines; Lymphocytes; Inflammation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400707

Metabolic fate of glutamine in lymphocytes, macrophages and neutrophils
Provedor de dados: 56
Autores: Curi,R.; Newsholme,P.; Pithon-Curi,T.C.; Pires-de-Melo,M.; Garcia,C.; Homem-de-Bittencourt Jr.,P.I.; Guimarães,A.R.P.
Eric Newsholme's laboratory was the first to show glutamine utilization by lymphocytes and macrophages. Recently, we have found that neutrophils also utilize glutamine. This amino acid has been shown to play a role in lymphocyte proliferation, cytokine production by lymphocytes and macrophages and phagocytosis and superoxide production by macrophages and neutrophils. Knowledge of the metabolic fate of glutamine in these cells is important for the understanding of the role and function of this amino acid in the maintenance of the proliferative, phagocytic and secretory capacities of these cells. Glutamine and glucose are poorly oxidized by these cells and might produce important precursors for DNA, RNA, protein and lipid synthesis. The high rate of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamine; Lymphocytes; Macrophages; Neutrophils; Metabolic fate.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100002

Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
Provedor de dados: 56
Autores: Machado,C.G.; Calado,R.T.; Garcia,A.B.; Falcão,R.P.
The multidrug resistance P-glycoprotein is a transmembrane efflux pump expressed by lymphocytes and is involved in their cytolytic activity. In the present study, we investigated the age-related changes of P-glycoprotein function in normal peripheral blood lymphocytes. Blood samples from 90 normal volunteers (age range, 0 to 86 years) were analyzed. P-glycoprotein function was assessed by the flow cytometric rhodamine 123 assay. P-glycoprotein function was highest in cord blood and progressively declined with age in peripheral blood T CD4+ and CD8+ cells. In contrast, P-glycoprotein function did not vary with age in CD19+ B or CD16+CD56+ natural killer cells. These data suggest that the decline in P-glycoprotein function in T CD4+ and CD8+ lymphocytes as a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P-glycoprotein; Multidrug resistance; Lymphocytes; Rhodamine 123; Flow cytometry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006

Inflammatory response of the spinal cord to multiple episodes of blood-brain barrier disruption and toxic demyelination in Wistar rats
Provedor de dados: 56
Autores: Fernandes,C.G.; Graça,D.L.; Pereira,L.A.V.D.
Multiple episodes of blood-brain barrier disruption were induced by sequential intraspinal injections of ethidium bromide. In addition to the barrier disruption, there was toxic demyelination and exposure of myelin components to the immune system. Twenty-seven 3-month-old Wistar rats received 2, 3 or 4 injections of 1 µl of either 0.1% ethidium bromide in normal saline (19 rats) or 0.9% saline (8 rats) at different levels of the spinal cord. The time intervals between the injections ranged from 28 to 42 days. Ten days after the last injection, all rats were perfused with 2.5% glutaraldehyde. The spinal sections were evaluated macroscopically and by light and transmission electron microscopy. All the lesions demonstrated a mononuclear phagocytic infiltrate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocytes; Multiple sclerosis; Central nervous system; Blood-brain barrier; Ethidium bromide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700008

Polymerase chain reaction detection of adenovirus DNA sequences in human lymphocytes
Provedor de dados: 58
Autores: Araújo,Aufra A.; Yokosawa,Jonny; Durigon,Edison L.; Ventura,Armando M.
Human lymphoid cells frequently carry adenoviruses and DNA sequences have been identified in peripheral blood lymphocytes by Southern blot and PCR, although these cells are not permissive for virus replication, suggesting persistence of the viral genome. In order to investigate this phenomenon we screened non-symptomatic volunteers for adenovirus DNA presence and E1A gene expression. DNA samples extracted from peripheral blood mononuclear cells of 51 volunteers were submitted to PCR using primers for a conserved hexon sequence, followed by nested PCR. Adenovirus sequences were detected in 27 samples (52.9%). After more than one year, new samples of these positive volunteers were analyzed and in 70.8% of the cases the result was maintained. Since this could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adenoviral infection; Viral persistence; Lymphocytes; Nested PCR assay.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200017

Immunohistochemical expression of B and T-lymphocytes and TGF-β in experimentally transplanted canine venereal tumor
Provedor de dados: 65
Autores: Silveira,Ana Carolina Trompieri; Gerardi,Daniel; Mouro,Juliana Vitti; Costa,Mirela Tinucci; Alessi,Antonio Carlos.
The tumor/host relationship may have a determining role in the progression or remission of a tumor. Greater infiltration of leukocytes into tumors has been associated with a better prognosis, although controversy regarding whether these cells have a central role in antitumor immunity still exists. Canine transmissible venereal tumor (TVT) is an experimentally transplantable type of tumor that has been used as an experimental model for the tumor/host relationship. The aim of this study was to evaluate the infiltration of T-lymphocytes (CD3, CD4, CD8) and B lymphocytes (CD79-α) and the expression of the cytokine TGF-β in TVT, by means of immunohistochemistry (ABC method). The experimental tumors were composed of puppies that developed TVT after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocytes; TVT; Immunohistochemistry; Dog.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000400029