Consulta: subjectFacets:"M. fortuitum"
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Data/hora: 12/06/2024 16:00:08

An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
Provedor de dados: Brazilian Journal of Microbiology
Autores: Marassi,Carla Dray; Silva,Marley; Oelemann,Walter Martin Roland; Fonseca,Leila de Souza; Ristow,Paula; Lilenbaum,Walter.
ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ &gt; 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacteria; ELISA; M. fortuitum; M. phlei.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300019

Evaluation of INNO-LiPA mycobacteria v2 assay for identification of rapidly growing mycobacteria
Provedor de dados: Brazilian Journal of Microbiology
Autores: García-Agudo,Lidia; Jesús,Iría; Rodríguez-Iglesias,Manuel; García-Martos,Pedro.
A total of 54 rapidly growing mycobacteria (RGM) isolated from patients attended in the two hospitals of Cádiz Bay (Spain) were selected during a seven-year-period (2000-2006) in order to evaluate the INNO-LiPA Mycobacteria v2 assay for mycobacterial identification, based on the reverse hybridization principle. The strains were cultured in Löwenstein-Jensen and Middlebrook 7H9 media and identified to the species level by sequencing of the 16S rRNA, PCR-restriction enzyme analysis of the hsp65 gene, conventional tests and INNO-LiPA Mycobacteria v2 assay. By the molecular methods we identified a total of 12 different species: 23 Mycobacterium fortuitum, 11 M. chelonae, 10 M. abscessus, 2 M. senegalense, 1 M. alvei, 1 M. brumae, 1 M. mageritense, 1 M....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacteria; Rapidly growing mycobacteria; M. fortuitum; M. chelonae; M. abscessus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300048