Consulta: subjectFacets:"MRSA"
Registros recuperados: 35
Data/hora: 09/06/2024 01:46:56

The Ancestral N-Terminal Domain of Big Defensins Drives Bacterially Triggered Assembly into Antimicrobial Nanonets
Provedor de dados: 5
Autores: Loth, Karine; Vergnes, Agnes; Barreto, Cairé; Voisin, Sébastien N; Meudal, Hervé; Da Silva, Jennifer; Bressan, Albert; Belmadi, Nawal; Bachère, Evelyne; Aucagne, Vincent; Cazevielle, Chantal; Marchandin, Hélène; Rosa, Rafael Diego; Bulet, Philippe; Touqui, Lhousseine; Delmas, Agnès F.; Destoumieux-garzón, Delphine.
Big defensins, ancestors of β-defensins, are composed of a β-defensin-like C-terminal domain and a globular hydrophobic ancestral N-terminal domain. This unique structure is found in a limited number of phylogenetically distant species, including mollusks, ancestral chelicerates, and early-branching cephalochordates, mostly living in marine environments. One puzzling evolutionary issue concerns the advantage for these species of having maintained a hydrophobic domain lost during evolution toward β-defensins. Using native ligation chemistry, we produced the oyster Crassostrea gigas BigDef1 (Cg-BigDef1) and its separate domains. Cg-BigDef1 showed salt-stable and broad-range bactericidal activity, including against multidrug-resistant human clinical isolates...
Tipo: Text
Palavras-chave: MRSA; Antimicrobial peptides; Antimicrobial resistance; Defensins; Fibrils; Innate immunity; Mechanisms of action; Nuclear magnetic resonance.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00588/70057/68000.pdf

Nasal carriage of resistant Staphylococcus aureus in a medical student community
Provedor de dados: 42
Autores: GUSHIKEN,CAROLINA Y.; MEDEIROS,LILIANE B.; CORREIA,BRUNA P.; SOUZA,JOYCE M.; MORIS,DANIELA V.; PEREIRA,VALERIA C.; GIUFFRIDA,ROGERIO; RODRIGUES,MARCUS V.P.
ABSTRACT Staphylococcus aureus can cause a variety of infections due to its high transmissibility, high pathogenic potential and resistance to multiple drugs, factors that contribute to the relevance of infections in healthcare services. The aim of this study was to document phenotypic and genotypic resistance factors of Staphylococcus aureus strains, isolated from nasal mucosa of medical students. A nasal swab was collected from the nares (nostrils) of 222 medical students. After collection, the samples were submitted to isolation and identification procedures. From 204 valid samples, 20.6% (42 samples) were positive for S. aureus. For the assessment of phenotypic resistance by disk-diffusion technique, from 42 samples, 95.2% showed resistance to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multidrug resistance; Colonization; MLSB resistance; MRSA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401501

Detecção dos genes de Staphylococcus aureus, enterotoxinas e de resistência à meticilina em leite
Provedor de dados: 45
Autores: Dias,N.L.; Silva,D.C.B.; Oliveira,D.C.B.S.; Fonseca Junior,A.A.; Sales,M.L.; Silva,N.
Realizou-se a detecção do gene de Staphylococcus aureus, de enterotoxinas e de resistência à meticilina com extração de DNA feita diretamente de amostras de leite. Das 200 amostras estudadas, 145 (72,5%) amplificaram o gene femA, e estas foram analisadas quanto à presença dos genes sea, seb, sec e mecA. Os genes das enterotoxinas mais prevalentes foram: sea (60%), seb (37,9%) e sec (6,9%). Foram encontradas 18 amostras de leite (11,0 %) com S. aureus portadores do gene mecA. A detecção de S. aureus diretamente do leite, sem a necessidade de isolamento bacteriano e a caracterização do potencial enterotoxigênico, demonstra que a técnica de PCR é muito útil para estudos epidemiológicos das infecções estafilocócicas da glândula mamária. O alto percentual...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Enterotoxinas; MRSA; PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600036

Application of RAPD-PCR for Determining the Clonality of Methicillin Resistant Staphylococcus aureus Isolated from Different Hospitals
Provedor de dados: 52
Autores: Idil,Neslihan; Bilkay,Isil Seyis.
Randomly amplified polymorphic DNA (RAPD)-PCR was applied with ten random 10-mer primers to examine the molecular diversity among methicillin resistant Staphylococcus aureus (MRSA) strains in the hospitals and to investigate the epidemiological spread of these strains between different hospitals. The main objective of the study was to identify appropriate primers, which successfully established the clonality of MRSA. Three of the primers yielded particularly discriminatory patterns and they were used to perform the RAPD analysis which revealed different bands ranging from 200 to 1500 bp. Dendogram was created by the un-weighted pair group method using arithmetic (UPGMA) average clustering and it was constructed based on the combination results of the new...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; RAPD-PCR; Clonal dissemination; UPGMA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400548

Local circulating clones of Staphylococcus aureus in Ecuador
Provedor de dados: 55
Autores: Zurita,Jeannete; Barba,Pedro; Ortega-Paredes,David; Mora,Marcelo; Rivadeneira,Sebastián.
ABSTRACT The spread of pandemic Staphylococcus aureus clones, mainly methicillin-resistant S. aureus (MRSA), must be kept under surveillance to assemble an accurate, local epidemiological analysis. In Ecuador, the prevalence of the USA300 Latin American variant clone (USA300-LV) is well known; however, there is little information about other circulating clones. The aim of this work was to identify the sequence types (ST) using a Multiple-Locus Variable number tandem repeat Analysis 14-locus genotyping approach. We analyzed 132 S. aureus strains that were recovered from 2005 to 2013 and isolated in several clinical settings in Quito, Ecuador. MRSA isolates composed 46.97% (62/132) of the study population. Within MRSA, 37 isolates were related to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; MSSA; Epidemic clones; MLVA typing.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600525

Characterization of the Brazilian endemic clone of methicillin-resistant Staphylococcus aureus (MRSA) from hospitals throughout Brazil
Provedor de dados: 55
Autores: Oliveira,Geraldo A.; Faria,Juarez B.; Levyand,Carlos E.; Mamizuka,Elsa M.
The objective of this study was to characterize patterns of the Brazilian endemic clone of methicillin-resistant Staphylococcus aureus (MRSA) from hospitals throughout Brazil. We studied 83 MRSA strains isolated from patients hospitalized in 27 public and private hospitals in 19 cities located in 14 Brazilian states from September, 1995, to June, 1997. The MRSA strains were typed using antibiograms, bacteriophage typing and pulsed field gel electrophoresis (PFGE). The analysis of genomic DNA by PFGE showed that 65 isolates presented the same PFGE pattern. This pattern was present in all of the hospitals studied indicating the presence of an endemic MRSA clone widely disseminated throughout Brazilian hospitals (BEC). All isolates belonging to the BEC proved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococus aureus; MRSA; Epidemiology; Brazilian endemic clone.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000400001

Detection of the mecA gene and identification of Staphylococcus directly from blood culture bottles by multiplex polymerase chain reaction
Provedor de dados: 55
Autores: Rocchetti,Taisa Trevizani; Martins,Katheryne Benini; Martins,Patricia Yoshida Faccioli; Oliveira,Rogério Antonio de; Mondelli,Alessandro Lia; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da.
ABSTRACT  Introduction: Staphylococcus spp. – both S. aureus, including methicillin-resistant strains (MRSA) and coagulase negative staphylococci (CoNS) – are relevant agents of healthcare-associated infections. Therefore, the rapid recognition of MRSA and methicillin-resistant CoNS from blood stream infections is critically important for patient management. It is worth noting that inappropriate empiric therapy has been associated with higher in-hospital mortality.  Material and methods: In this study we evaluated a multiplex polymerase chain reaction (multiplex PCR) standardized to detect Staphylococcus spp., S. aureus, and mecA gene-encoded oxacillin resistance directly from blood culture bottles. A total of 371 blood cultures with Gram-positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus spp.; Staphylococcus aureus; MRSA; MecA gene; Blood cultures; Multiplex PCR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200099

Worrisome trends in rising minimum inhibitory concentration values of antibiotics against methicillin resistant Staphylococcus aureus - Insights from a tertiary care center, South India
Provedor de dados: 55
Autores: Niveditha,Nagasundaram; Sujatha,Sistla.
ABSTRACT  INTRODUCTION:  Appearance of isolated reports of resistance to anti-methicillin-resistantStaphylococcus aureus (MRSA) drugs is worrisome underscoring the need to continuously monitor the susceptibility of clinical MRSA isolates to these drugs. Hence, the present study is conducted to determine the susceptibility of MRSA isolates to various classes of anti-MRSA drugs such as vancomycin (glycopeptide), daptomycin (lipopeptide), tigecycline (glycylcycline), and linezolid (oxazolidinone) to determine the MIC50 and MIC90 values, and to observe MIC creep over a three year period, if any, with respect to these drugs.  METHODS:  A total of 200 isolates of MRSA obtained from clinical specimens were included. MIC was determined by E-test for anti-MRSA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; VISA; MIC creep; Vancomycin.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600585

Molecular epidemiology of heteroresistant vancomycin-intermediate Staphylococcus aureus in Brazil
Provedor de dados: 55
Autores: Silveira,Alessandro Conrado de Oliveira; Cunha,Gabriela Rosa da; Caierão,Juliana; Cordova,Caio Mauricio Mendes de; d'Azevedo,Pedro Alves.
ABSTRACTTo determine the epidemiological and molecular characteristics of 12 Staphylococcus aureus isolates presenting heteroresistance to vancomycin in laboratories of two cities in Santa Catarina, southern Brazil. Epidemiological data, including the city of isolation, health institution, and date of isolation were considered, as well as the associated clinical specimen. For molecular characterization, we analyzed the staphylococcal cassette chromosome types, the erm gene presence, and the genomic diversity of isolates using pulsed-field gel electrophoresis. The 12 isolates of S. aureus were previously confirmed as heteroresistance to vancomycin using the population analysis profile-area under curve. Regarding genetic variability, two clones were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Small colony variants; Antimicrobial resistance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500466

Epidemiology and surveillance of methicillin-resistant staphylococcus aureus in Latin America
Provedor de dados: 55
Autores: Mejía,Carlos; Zurita,Jeannete; Guzmán-Blanco,Manuel.
Surveillance systems monitoring the spread and divergence of methicillin-resistant Staphylococcus aureus (MRSA) strains are critical if preventive and therapeutic measures targeting MRSA infection are to be employed optimally. Surveillance provides information on the spread of MRSA, on the emergence of new strains within hospitals and communities, on the antibiotic resistance profile and virulence of strains, and on the risk factors associated with infection. These data help clinicians to provide appropriate empiric treatment of infections circulating in their region, leading to improved patient outcomes. While information on MRSA epidemiology in Latin America is growing, significant gaps exist in the available data, especially in local areas where fewer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Epidemiology surveillance Latin America resistance.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000800003

Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) among patients visiting the emergency room at a tertiary hospital in Brazil
Provedor de dados: 55
Autores: Ribeiro,Julival; Boyce,John M.; Zancanaro,Pedro Q.
We surveyed patients seen at the emergency room at a tertiary hospital in Brazil from August to November 1997. All patients' (n = 600) anterior nares were cultured for MRSA; the results were confirmed by oxacillin disk diffusion methods and by detection of the mecA gene with PCR. Bacteria were found in 93.3% (560) of the patients and MRSA in 0.7% (n = 4). One patient had community-acquired MRSA. We concluded that MRSA is uncommon among patients visiting the emergency room. The presence of community-acquired MRSA can lead to serious medical and epidemiological issues, although initial clinical presentation may not differ from that of infections with other staphylococci. The empirical use of vancomycin for suspected community-acquired infections is seldom...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Staphylococcus aureus; Emergency room; Brazil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100009

Molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in two metropolitan areas of São Paulo State, southeast Brazil
Provedor de dados: 55
Autores: Cury,Gisele Gentile; Mobilon,Cristiane; Stehling,Eliana Guedes; Lancellotti,Marcelo; Ramos,Marcelo de Carvalho; Martinez,Roberto; Brocchi,Marcelo; Silveira,Wanderley Dias da.
One hundred and fifty-one methicillin-resistant z (MRSA) strains have been isolated from patients admitted in tertiary care hospitals in two metropolitan areas (Campinas City and Ribeirão Preto City) in the southeast region of Brazil and analyzed through PCR-based techniques [(PCR amplification of spa, coa, and housekeeping genes (arcC, aroE, gmk, pta, tpi, yqiL)] and further restriction fragment typing of coa and of housekeeping genes. The heterogeneity of spa gene was determined directly by agarose gel electrophoresis migration. The results obtained indicate the existence of three (A, B, C) main clusters. Since the strain distribution in these three clusters is much characteristic, it denotes the existence of three main clones. All strains isolated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Nosocomial; Molecular characterization; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300002

Temporal evolution of the prevalence of methicillin-resistant Staphylococcus aureus in a tertiary hospital in Bahia, Brazil: a nine-year evaluation study
Provedor de dados: 55
Autores: Brites,Carlos; Silva,Nanci; Sampaio- Sá,Márcia.
Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have become an increasing problem in Brazilian hospitals within the last years. In Bahia, there is scarce information on the epidemiological characteristics of MRSA infections and their determinants. The objective of this study was to evaluate the temporal evolution of MRSA infections in a private, tertiary hospital, in Salvador, the capital of the state of Bahia. We reviewed the microbiological records of bacterial isolates from the Hospital Espanhol, a 300 bed, general hospital, in Salvador. We analyzed the frequency of positive cultures for S. aureus during the last nine years, the prevalence of MRSA, and the spatial distribution of the isolates in the hospital. We also evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; MRSA; Bacterial resistance.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000400003

Methicillin-resistant Staphylococcus aureus isolated from an intensive care unit in Minas Gerais, Brazil, over a six-year period
Provedor de dados: 55
Autores: Nascimento,Thiago C.; Diniz,Cláudio G.; Silva,Vânia L.; Ferreira-Machado,Alessandra B.; Fajardo,Marina O.; de Oliveira,Tamara Lopes R.; Ferreira,Dennis de C.; Cavalcante,Fernanda S.; Santos,Kátia R. Netto dos.
ABSTRACT To characterize methicillin-resistant Staphylococcus aureus isolates from an intensive care unit of a tertiary-care teaching hospital, between 2005 and 2010. A total of 45 isolates were recovered from patients admitted to the intensive care unit in the study period. Resistance rates higher than 80% were found for clindamycin (100%), erythromycin (100%), levofloxacin (100%), azithromycin (97.7%), rifampin (88.8%), and gentamycin (86.6%). The SCCmec typing revealed that the isolates harbored the types III (66.7%), II (17.8%), IV (4.4%), and I (2.2%). Four (8.9%) isolates carried non-typeable cassettes. Most (66.7%) of the isolates were related to the Brazilian endemic clone from CC8/SCCmec III, which was prevalent (89.3%) between 2005 and 2007,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Clonal complex; MRSA; PFGE lineages; SCCmec.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000100055

Diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus in Latin America
Provedor de dados: 55
Autores: Zurita,Jeannete; Mejía,Carlos; Guzmán-Blanco,Manuel.
Strategies to monitor and control the spread of methicillin-resistant Staphylococcus aureus (MRSA) infections are dependent on accurate and timely diagnosis of MRSA in both hospital and community settings. In Latin America, significant diversity in diagnostic and susceptibility testing procedures exists at the regional, national and local levels. Various tests for S. aureus and MRSA are available in clinical settings, but the application of these techniques differs between and within countries, and quality control measures are not uniformly applied to verify diagnoses. To optimize the diagnosis of MRSA infections across Latin America, a more consistent approach is required. This may include: adoption and appropriate adaption of specific guidelines for MRSA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Diagnosis; Susceptibility testing; Latin America.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000800005

Efficacy of a program of prevention and control for methicillin-resistant Staphylococcus aureus infections in an intensive-care unit
Provedor de dados: 55
Autores: Moreira,Marina; Freitas,Marise R.; Martins,Sinaida T.; Castelo,Adauto; Medeiros,Eduardo Alexandrino Servolo.
Methicillin-resistant Staphylococcus aureus (MRSA) is endemic in most Brazilian Hospitals, and there are few studies which show the efficacy of control measures in such situations. This study evaluated intensive care unit (ICU) patients, in two years divided in control, intervention and post-intervention group. Intervention measures: hands-on educational programs for healthcare workers; early identification of MRSA infected or colonized patients, labeled with a bed-identification tag for contact isolation; nasal carriers, patients, and healthcare professionals treated with topical mupirocin for five days. The hospital infection rates in the control period were compared to the ones in the post-intervention period. Hospital infection rates were found by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Methicillin-resistant Staphylococcus aureus; Intensive care unit infections.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100015

The changing pattern of methicillin-resistant staphylococcus aureus clones in Latin America: implications for clinical practice in the region
Provedor de dados: 55
Autores: Rodríguez-Noriega,Eduardo; Seas,Carlos.
Methicillin-resistant Staphylococcus aureus (MRSA) clones belonging to the Brazilian, Pediatric, Cordobes/Chilean and New York/Japan clonal complexes are widely distributed across Latin America, although their individual distribution patterns and resistance to antimicrobial drugs are constantly changing. Furthermore, clones with increased virulence are beginning to appear more frequently both in hospital and community settings, and there is evidence that virulence factors can be transferred between hospital- and community-associated clones through recombination. These changing patterns have significant implications for clinical practice in the region. Most importantly, clinicians need to be aware of the changing antimicrobial resistance profile of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Clones; Molecular epidemiology; Latin America.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000800004

Detection of methicillin resistance in Staphylococcus aureus isolated from pediatric patients: is the cefoxitin disk diffusion test accurate enough?
Provedor de dados: 55
Autores: Mimica,M.J.; Berezin,E.N.; Carvalho,R.L.B.; Mimica,I.M.; Mimica,L.M.J.; Sáfadi,M.A.P.; Schneider,E.; Caiaffa-Filho,H.H.
We evaluated the performance of several methods for the detection of methicillin resistance in Staphylococcus aureus using 101 clinical S. aureus isolates from pediatric patients in a tertiary hospital in Brazil; 50 isolates were mecA-positive and 51 were mecA-negative. The Etest and oxacillin agar screening plates were 100% sensitive and specific for mecA presence. Oxacillin and cefoxitin disks gave sensitivities of 96 and 92%, respectively, and 98% specificity. Alterations of CLSI cefoxitin breakpoints increased sensitivity to 98%, without decreasing specificity. Our results highlight the importance of a continuing evaluation of the recommended microbiological methods by different laboratories and in different settings. If necessary, laboratories should...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Disc diffusion; Cefoxitin; MecA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000400009

Treatment of methicillin-resistant Staphylococcus aureus in Latin America
Provedor de dados: 55
Autores: Luna,Carlos M; Rodríguez-Noriega,Eduardo; Bavestrello,Luis; Gotuzzo,Eduardo.
The global spread of methicillin-resistant Staphylococcus aureus (MRSA) means it is now a pathogen of worldwide public health concern. Within Latin America, MRSA is highly prevalent, with the proportion of S. aureus isolates that are methicillin-resistant on the rise, yet resources for managing the infection are limited. While several guidelines exist for the treatment of MRSA infections, many are written for the North American or European setting and need adaptation for use in Latin America. In this article, we aim to emphasize the importance of appropriate treatment of MRSA in the healthcare and community settings of Latin America. We present a summary of the available guidelines and antibiotics, and discuss particular considerations for clinicians...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Treatment; Antibiotic therapy; Latin America.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000800007

MRSA outbreak at a transplantation unit
Provedor de dados: 55
Autores: Romanelli,RMC; Clemente,WT; Lima,SSS; Rezende,EM; Martinho,GH; Paiva,LFR; Neves,FAC; Madeira,JGC; Amâncio,GCS; Lima,AS; Faria,LC; Coutinho,RL.
Methicillin-resistant Staphylococcus aureus (MRSA) infections frequently complicate the post-operative course of transplant recipients, and despite nasal carriage and endemic colonization, MRSA outbreaks are not commonly described. This study reports a case of MRSA outbreak and discusses infection control measures and recommendations for this situation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Outbreak; Surgical site infection; Liver transplantation; RAPD.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100011

Prevention strategies for methicillin-resistant Staphylococcus aureus (MRSA) in Latin America
Provedor de dados: 55
Autores: Alvarez,Carlos; Labarca,Jaime; Salles,Mauro.
After the first reports of the emergence of methicillin-resistant Staphylococcus aureus (MRSA) in the 1970s, numerous measures intended to prevent its transmission were initiated in hospitals. However, in most cases, large-scale measures failed to be implemented and the transmission of MRSA has since led to a global pandemic. Presently, doubts still remain about the best approach to prevent and control MRSA and more often than not, control measures are not implemented. Therefore, we review here the current situation in Latin America with respect to existing policies for control of MRSA, and evaluate the evidence for control measures in hospitals and the community. We look at the risk factors for infection and transmission of MRSA between hospital patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Prevention; Infection control; Latin America.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000800006

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from a Brazilian university hospital
Provedor de dados: 55
Autores: Martins,André; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Cunha,Maria de Lourdes Ribeiro de Souza da.
The aim of this study was to perform SCCmec typing in Staphylococcus aureus isolates and to characterize the clonal profile of these isolates. Forty-six mecA gene-positive strains isolated between 2002 and 2006 were submitted to antimicrobial resistance testing by the E-test, SCCmec typing by multiplex PCR, and clonal profile analysis by pulsed-field gel electrophoresis. Forty-one (89.1%) isolates were typed as SCCmec III and five (10.9%) as SCCmec IV. Four circulating clones were detected, one of them comprising isolates related to the Brazilian epidemic clone. This clone was detected throughout the study period. The SCCmec III isolates were associated with a high rate of multidrug resistance and clonal dissemination of methicillin-resistant S. aureus in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Staphylococcus aureus; MRSA; SCCmec; BEC.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300331

In vitro susceptibility of methicillin-resistant Staphylococcus aureus isolates from skin and soft tissue infections to vancomycin, daptomycin, linezolid and tedizolid
Provedor de dados: 55
Autores: Múnera,Johanna Marcela Vanegas; Ríos,Ana María Ocampo; Urrego,Daniela Montoya; Jiménez Quiceno,Judy Natalia.
Abstract  Introduction Treatment of multidrug-resistant Gram-positive infections caused by Staphylococcus aureus remains as a clinical challenge due to emergence of new resistance mechanisms. Tedizolid is a next-generation oxazolidinone, recently approved for skin and soft tissues infections. We conducted a study to determine in vitro susceptibility to vancomycin, daptomycin, linezolid and tedizolid in MRSA clinical isolates from adult patients with skin and soft tissue infections.  Material and methods Methicillin-resistant S. aureus isolates were collected in three tertiary-care hospitals of Medellin, Colombia, from February 2008 to June 2010 as part of a previous study. Clinical characteristics were assessed by medical records and MIC values were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Skin and soft tissues infections; Tedizolid; Linezolid; Vancomycin; Daptomycin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500493

Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil
Provedor de dados: 56
Autores: Sousa-Junior,F.C. de; Silva-Carvalho,M.C.; Fernandes,M.J.B.C.; Vieira,M.F.P.; Pellegrino,F.L.P.C.; Figueiredo,A.M.S.; Melo,M.C.N. de; Milan,E.P.
Methicillin-resistant Staphylococcus aureus (MRSA) is a major agent of hospital infections worldwide. In Brazil, a multiresistant MRSA lineage (ST239-SCCmecIIIA), the so-called Brazilian epidemic clone (BEC), has predominated in all regions. However, an increase in nosocomial infections caused by non-multiresistant MRSA clones has recently been observed. In the present study, 45 clinical isolates of MRSA obtained from a university hospital located in Natal city, Brazil, were identified by standard laboratory methods and molecularly characterized using staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was carried out using CLSI methods. The MRSA isolates studied displayed a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methicillin-resistant Staphylococcus aureus; MRSA; Antimicrobial resistance; Brazilian epidemic clone; Pediatric clone; SCCmecIV.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000002

Emergence of clonal complex 5 (CC5) methicillin-resistant Staphylococcus aureus (MRSA) isolates susceptible to trimethoprim-sulfamethoxazole in a Brazilian hospital
Provedor de dados: 56
Autores: Teixeira,M.M.; Araújo,M.C.; Silva-Carvalho,M.C.; Beltrame,C.O.; Oliveira,C.C.H.B.; Figueiredo,A.M.S.; Oliveira,A.G.
In this study, genotyping techniques including staphylococcal chromosomal cassette mec (SCCmec) typing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and restriction-modification tests were used to compare the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered at two times within a 10-year interval (1998 and 2008) from a tertiary Brazilian hospital. In addition, the antimicrobial susceptibility profiles were analyzed. All 48 MRSA isolates from 1998 and 85.7% from 2008 (48/56 isolates) displayed multidrug-resistance phenotypes and SCCmec III. All but one of the 13 representative SCCmec III isolates belonged to CC8 and had PFGE patterns similar to that of the BMB9393 strain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Antimicrobial susceptibility; CC5; Cordobes clone; Trimethoprim-sulfamethoxazole.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700011

The first report in Brazil of severe infection caused by community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA)
Provedor de dados: 56
Autores: Rozenbaum,R.; Sampaio,M.G.; Batista,G.S.; Garibaldi,A.M.; Terra,G.M.F.; Souza,M.J.; Vieira,E.N.; Silva-Carvalho,M.C.; Teixeira,L.A.; Figueiredo,A.M.S.
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emergent pathogen in Brazil. However, there are no data on the prevalence of CA-MRSA. We report here the first well-characterized case of severe life-threatening CA-MRSA infection in a child living in Rio de Janeiro city. The patient had many complications including hematogenous osteomyelitis and involvement of multiple sites requiring drainage of soft-tissue abscess, and pleural and pericardial empyema. The MRSA isolates recovered were genotyped using PFGE, SCCmec typing and multilocus sequence typing. Disk diffusion tests were performed following Clinical and Laboratory Standards Institute recommendations. In addition, the presence of Panton-Valentine leukocidin (PVL) was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CA-MRSA; MRSA; PVL; S. aureus; Antimicrobial resistance.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800011

Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus
Provedor de dados: 56
Autores: Beltrame,C. O.; Côrtes,M. F.; Bandeira,P. T.; Figueiredo,A. M. S.
Biofilm formed by Staphylococcus aureus is considered an important virulence trait in the pathogenesis of infections associated with implantable medical devices. Gene expression analyses are important strategies for determining the mechanisms involved in production and regulation of biofilm. Obtaining intact RNA preparations is the first and most critical step for these studies. In this article, we describe an optimized protocol for obtaining total RNA from sessile cells of S. aureus using the RNeasy Mini Kit. This method essentially consists of a few steps, as follows: 1) addition of acetone-ethanol to sessile cells, 2) lysis with lysostaphin at 37°C/10 min, 3) vigorous mixing, 4) three cycles of freezing and thawing, and 5) purification of the lysate in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA preparation; MRSA; Biofilm; Sessile cells; Staphylococcus aureus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201071

Mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swab specimens in Brazil
Provedor de dados: 58
Autores: Santos,Danielle Caldeira Martins dos; Costa,Thaina Miranda da; Rabello,Renata Fernandes; Alves,Fábio Aguiar; Mondino,Silvia Susana Bona de.
The isolation of mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swabs is reported. Among the 59 isolates, 9 (15%) isolates were mannitol-negative; all of these isolates were categorized as staphylococcal cassette chromosome mec (SCCmec) type IVa. This report emphasizes that mannitol fermentation on mannitol salt agar should not be used as the sole criterion when screening nasal swab specimens for S. aureus.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mannitol-negative; MRSA; Nasal swab surveillance; SCCmec.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200531

Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil
Provedor de dados: 58
Autores: Sousa Júnior,Francisco Canindé de; Néri,Gildelane da Silva; Silva,Ana Karine; Araújo,Bibiana Priscila Rodrigues Câmara de; Guerra,Myrian Júlia de Paiva Dourado; Fernandes,Maria José de Britto Costa; Milan,Eveline Pipolo; Melo,Maria Celeste Nunes de.
Many methods have been described for the detection of methicillin-resistant Staphylococcus aureus (MRSA), but the heterogeneous expression of methicillin resistance affects the reliability of these methods. The aim of the present study was to evaluate some methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil. Among the isolates, 15 were methicillin-susceptible and 45 were methicillin-resistant, including low-level heterogeneous resistance strains. Both the 30 µg-cefoxitin disk and PBP2a test had 100% sensibility/specificity and appear to be good options for the detection of MRSA in the clinical laboratory.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Methicillin resistance; Heterogeneous resistance; Cefoxitin; PBP2a.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200009

Conventional versus molecular tests (Multiplex PCR and PCR mecA gene) for detection of methicillin resistant Staphylococcus aureus
Provedor de dados: 58
Autores: Ribas,Rosineide Marques; Gontijo-Filho,Paulo Pinto; Darini,Ana Lúcia da Costa.
In this study, for detection of methicillin-resistant S. aureus (MRSA), a mecA multiplex PCR-based amplification was compared with the 1 µg oxacillin disk diffusion test, detection of minimal inhibitory concentration (MIC), and screening in agar with 4% NaCl and 6 µg/mL oxacillin. Among 24 isolates obtained from blood, mecA gene was detected in only 16 (66.7%) isolates by multiplex PCR. The MIC test showed a range of resistance to oxacillin from 0.19 to 512 µg/mL, among these isolates. Data obtained by screening and dilution tests showed that sensitivity to methicillin was 80.0% and 72.8%, respectively, when compared with the presence of mecA gene (multiplex). All isolates, including the negatives, when revaluated for mecA gene by PCR were positive....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiplex PCR; MRSA; Beta-lactamase; MecA gene.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500012

Epidemiology of diabetic foot infections in a reference tertiary hospital in India
Provedor de dados: 58
Autores: Saseedharan,Sanjith; Sahu,Manisa; Chaddha,Roonam; Pathrose,Edwin; Bal,Arun; Bhalekar,Pallavi; Sekar,Priyadharshini; Krishnan,Padma.
Abstract  Introduction The present study attempts to examine the microbial profile and antibiotic susceptibility of diabetic foot infections in the intensive care unit of a tertiary referral centre for diabetic foot. As part of the study, we also attempted to find the prevalence of blaNDM-like gene among carbapenem-resistant gram negative infections.  Methodology A prospective study of 261 patients with diabetic foot infections was performed during the period between January 2014 and June 2014.  Results A total of 289 isolates were obtained from 178 tissue samples from 261 patients, 156 (59.7%) males and 105 (40.2%) females, with a mean age of 58 years (-15 years), having diabetic foot infection. No growth was seen in thirty eight (17.6%) tissue samples....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetic foot infection; BlaNDM; MRSA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200401

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from blood in Rio de Janeiro displaying susceptibility profiles to non-β-lactam antibiotics
Provedor de dados: 58
Autores: Zuma,Alexandra Vidal Pedinotti; Lima,Danielle Ferreira; Assef,Ana Paula D'Alincourt Carvalho; Marques,Elizabeth Andrade; Leão,Robson Souza.
Abstract The distinction between healthcare-associated MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA) infections has become increasingly blurred. We assessed the molecular characterization and antimicrobial resistance profile for MRSA isolates from blood. Most of all (81.9%) isolates are related to known HA-MRSA and CA-MRSA epidemic lineages, such as, USA300, USA400, USA600, USA800 and USA1100. This is the first multicenter study in Rio de Janeiro.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; MLST; USA800; USA400; USA1100.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200237

Antimicrobial activity of allylic thiocyanates derived from the Morita-Baylis-Hillman reaction
Provedor de dados: 58
Autores: Sá,Marcus Mandolesi; Ferreira,Misael; Lima,Emerson Silva; Santos,Ivanildes dos; Orlandi,Patrícia Puccinelli; Fernandes,Luciano.
Bacterial resistance to commonly used antibiotics has been recognized as a significant global health issue. In this study, we carried out the screening of a family of allylic thiocyanates for their action against a diversity of bacteria and fungi with a view to developing new antimicrobial agents. Allylic thiocyanates bearing halogenated aryl groups, which were readily obtained in two steps from the Morita-Baylis-Hillman adducts, showed moderate-to-high activity against selective pathogens, including a methicillin-resistant S. aureus (MRSA) strain. In particular cases, methyl (Z)-3-(2,4-dichlorophenyl)-2-(thiocyanomethyl)-2-propenoate exhibited antimicrobial activity comparable to the reference antibiotic Imipenem.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial; Allylic thiocyanate; Morita-Baylis-Hillman reaction; MRSA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300007

Antibacterial susceptibility patterns and cross-resistance of methicillin resistant and sensitive Staphyloccus aureus isolated from the hospitalized patients in Shiraz, Iran
Provedor de dados: 58
Autores: Japoni,Aziz; Ziyaeyan,Mazyar; Jmalidoust,Marzieh; Farshad,Shohreh; Alborzi,Abdolvahab; Rafaatpour,Noraladdin; Badiee,Parisa.
Nosocomial infections caused by methicillin-resistant staphylococci (MRSA) pose a serious problem in many countries. This study aimed to determine the antibacterial susceptibility patterns of methicillin sensitive and resistant Staphylococcus aureus isolates from the hospitalized patients. Totally 356 isolates of Staphylococcus aureus (S. aureus) including 200, 137 and 19 corresponding to MSSA, MRSA, and intermediate MRSA strains, respectively were isolated. Antibacterial susceptibility patterns of the isolates to 14 antibiotics were examined using Kirby-Bauer method. MICs of 15 antibiotics to 156 MRSA isolates were determined by E test method. Cross-resistances of MRSA isolates (137+19) to the other tested antibiotics were also determined. S.aureus with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Minimum inhibitory concentration; Empirical therapy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300005

Newly-synthesized chalcones-inhibition of adherence and biofilm formation of methicillin-resistant Staphylococcus aureus
Provedor de dados: 58
Autores: Bozic,Dragana D.; Milenkovic,Marina; Ivkovic,Branka; Cirkovic,Ivana.
Biofilm formation and adherence of bacteria to host tissue are one of the most important virulence factors of methicillin-resistant strains of Staphylococcus aureus (MRSA). The number of resistant strains is seriously increasing during the past years and bacteria have become resistant, not only to methicillin, but also to other commonly used antistaphylococcal antibiotics. There is a great need for discovering a novel antimicrobial agent for the treatment of staphylococcal infections. One of the most promising groups of compounds appears to be chalcones. In present study we evaluated the in vitro effect of three newly synthesized chalcones: 1,3-Bis-(2-hydroxy-phenyl)-propenone, 3-(3Hydroxy-phenyl)-1-(2-hydroxy-phenyl)-propenone and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chalcones; Glycocalyx; Biofilms; MRSA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100038