Consulta: subjectFacets:"Mannheimia haemolytica"
Registros recuperados: 5
Data/hora: 09/06/2024 19:04:57

A fast and simple assay to quantify bacterial leukotoxin activity
Provedor de dados: Electronic Journal of Biotechnology
Autores: Oppermann,Tobias; Schwarz,Stefan; Busse,Nadine; Czermak,Peter.
Background: Mannheimia haemolytica is the primary bacterial pathogen in causing bovine respiratory disease with tremendous annual losses in the cattle industry. The leukotoxin from M. haemolytica is the predominant virulence factor. Several leukotoxin activity assays are available but not standardized regarding sample preparation and cell line. Furthermore, these assays suffer from a high standard error, a prolonged time consumption and often complex sample pretreatments, which is important from the bioprocess engineering point of view. Results: Within this study, an activity assay based on the continuous cell line BL3.1 combined with a commercial available adenosine triphosphate viability assay kit was established. The leukotoxin activity was found to be...
Tipo: Journal article
Palavras-chave: ATP assay; Bacterial pathogen; Bovine respiratory disease; Cattle industry; Critical parameters; Lipopolysaccharides; Mannheimia haemolytica; Microbial biotechnology; Polymyxin B; Quantification; Virulence.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600006

Resistencia a antimicrobianos en cepas de Mannheimia haemolytica aisladas de exudado nasal de bovinos productores de leche
Provedor de dados: Revista Veterinaria México
Autores: Samaniego B.,María Luisa; Contreras J.,José Luis; Jaramillo-Arango,Carlos J.; Aguilar-Romero,Francisco; Vázquez Navarrete,Jesús; Hernández-Castro,Rigoberto; Suárez-Güemes F.,Francisco; Trigo Tavera,Francisco.
Se emplearon 201 cepas de Mannheimia haemolytica provenientes de muestras de exudado nasal de bovinos productores de leche, 123 de bovinos clínicamente sanos (CS) y 78 de bovinos enfermos de neumonía (CE), obtenidas de un complejo lechero en la región de Tizayuca, Hidalgo, México, las cuales fueron identificadas previamente mediante pruebas convencionales de cultivo y bioquímicas, y serotipificadas mediante la prueba de hemaglutinación indirecta. Se les realizó la prueba de difusión en placa para determinar la resistencia a diversos antimicrobianos como ampicilina, gentamicina, ceftiofiur, penicilina, estreptomicina, trimetoprim con sulfametoxazol, tetraciclina y eritromicina. Las frecuencias más altas en la resistencia a antimicrobianos se presentaron a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mannheimia haemolytica; Resistencia; Antimicrobianos; Antibiograma; Exudado nasal; Bovinos.
Ano: 2012
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922012000200004

Estudo clínico e laboratorial da pneumonia de bezerros induzida pela inoculação intrabronquial de Mannheimia haemolytica
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Fagliari,J.J.
Foram utilizados 14 bezerros da raça Holandesa entre 15 e 30 dias de idade, sete inoculados com 5ml de solução salina fosfato-tamponada Dulbecco (DPBS) e sete inoculados com 5x10(9) UFC de Mannheimia haemolytica/5 ml de DPBS, via intrabronquial. O exame clínico e as colheitas de sangue e de lavado broncoalveolar foram realizados antes e 4, 6, 12 e 24 horas após a inoculação. O exame histopatológico dos pulmões revelou lesões de pneumonia fibrinosa hemorrágica. Os sinais clínicos surgiram quatro horas após a inoculação das bactérias. Os sete bezerros enfermos apresentavam apatia, respiração abdominal, alteração dos ruídos respiratórios, hipertermia, elevação das freqüências cardíaca e respiratória e arritmia cardíaca. Os exames de laboratório mostraram...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bezerro; Pneumonia; Mannheimia haemolytica; Diagnóstico.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000100002

Ocorrência de Mannheimia haemolytica e de Pasteurella multocida em ovinos sadios e com enfermidade respiratória
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Viana,L.; Gonçalves,R.C.; Oliveira Filho,J.P.; Paes,A.C.; Amorim,R.M.
Frequency of Mannheimia haemolytica and Pasteurella multocida in the respiratory tract of lambs in the region of Botucatu, SP, Brazil, was studied. Nasopharingeal and oropharingeal swabs were obtained from 262 animals: 180 from healthy and 82 from animals with respiratory diseases. M. haemolytica was the most prevalent (47%), followed by the association of M. haemolytica and P. multocida (27%), and P. multocida (11%). Animals with respiratory disease presented higher occurrence of P. multocida in the nasopharynx as compared to healthy animals (P&lt;0.05). No significant difference in isolation rate of M. haemolytica, P. multocida, and association of these microorganisms in the oropharynx of healthy and affected animals was observed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovino; Mannheimia haemolytica; Pasteurella multocida; Doenças respiratória.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600035

Use of metaphylactic protocols based on the risk to develop bovine respiratory diseases in feedlot cattle
Provedor de dados: Ciência Rural
Autores: Magalhães,Layane Queiroz; Baptista,Anderson Lopes; Fonseca,Pedro de Almeida; Menezes,Guilherme Lobato; Nogueira,Geison Morel; Headley,Selwyn Arlington; Fritzen,Juliana Torres Tomazi; Alfieri,Amauri Alcindo; Saut,João Paulo Elsen.
ABSTRACT: Bovine respiratory diseases (BRD) affect production rates negatively because it compromise health and well-being of the affected animal. The hypothesis of this study was that the use of metaphylactic protocols based on the risk to develop BRD would reduce morbidity and pulmonary lesions. For this purpose, the aims of this study were to evaluate the effect of two metaphylactic protocols on the morbidity of feedlot cattle with a known sanitary history, occurrence of pulmonary lesions at slaughter, and the possible participation of Mannheimia haemolytica, Histophilus somni, Bovine alphaherpesvirus 1 (BoHV-1) and bovine respiratory syncytial virus (BRSV) in the development of BRD. An experimental study was designed in which 3,094 adult, male, cattle,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histophilus somni; Mannheimia haemolytica; Pulmonary lesions.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800454