Consulta: subjectFacets:"Mastitis"
Registros recuperados: 59
Data/hora: 11/06/2024 09:03:37

microRNAs and the mammary gland: a new understanding of gene expression
Provedor de dados: 74
Autores: Gigli,Isabel; Maizon,Daniel Omar.
MicroRNAs (miRNAs) have been identified in cells as well as in exosomes in biological fluids such as milk. In mammary gland, most of the miRNAs studied have functions related to immunity and show alterations in their pattern of expression during lactation. In mastitis, the inflammatory response caused by Streptococcus uberis alters the expression of miRNAs that may regulate the innate immune system. These small RNAs are stable at room temperature and are resistant to repeated freeze/thaw cycles, acidic conditions and degradation by RNAse, making them resistant to industrial procedures. These properties mean that miRNAs could have multiple applications in veterinary medicine and biotechnology. Indeed, lactoglobulin-free milk has been produced in transgenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactation; Mastitis; Milk; MiRNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400001

Sensibilidade antimicrobiana de Staphylococcus aureus isolados no leite de cabras com mastite subclínica.
Provedor de dados: 14
Autores: SOUZA, V. de; MARTINS, P. Y. F.; PINTO, D. dos S.; FERNANDES, D. R.; LIMA, A. R.
Os resultados obtidos ressaltam a importância da avaliação da sensibilidade antimicrobiana in vitro para cepas de Staphylococcus aureus isolados de casos de mastite, antes da indicação do tratamento para os animais, para dificultar a seleção de estirpes resistentes.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Bacterial diseases; Caprino; Cabra leiteira; Doença animal; Mamite; Staphylococcus Aureus; Bactéria; Antibiótico; Goats; Mastitis; Mammary gland diseases; Antibiotics.
Ano: 2017
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1087468

Mastite caprina: algumas medidas de prevenção.
Provedor de dados: 14
Autores: SILVA, E. R. da; ALVES, F. S. F.; PINHEIRO, R. R.
bitstream/item/26694/1/COT-31.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Prevention; Caprino; Mamite; Doença animal; Profilaxia; Goats; Mastitis; Animal diseases.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/515153

Uso da condutividade elétrica do leite para detecção de mastite subclínica caprina.
Provedor de dados: 14
Autores: SOUZA, V. de; LIMA, A. R.; MOURA, J. W. F.; ANGELO, F. F.; ALCINDO, J. F.; MESQUITA, F. L. T. de.
O trabalho avalia a utilização do teste da condutividade elétrica como ferramenta para o diagnóstico da mastite em amostras de leite obtidas em diferentes rebanhos de caprinos leiteiros.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Caprino leiteiro; Teste de Mamite; Doença Animal; Bactéria; Glândula Mamaria; Goat diseases; Brazil.; Mastitis; Bacterial infections; Disease diagnosis; Staphylococcus; Mammary gland diseases.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1116655

Relação entre contagem de células somáticas e a composição do leite de cabra.
Provedor de dados: 14
Autores: SOUZA, V. de; LIMA, A. R.; MARTINS, P. Y. F.; MOURA, J. W. F.; FERNANDES, D. R.; SILVA, L. M. F. da.
Diante da importância da qualidade do leite e dos derivados, esta publicação avalia a correlação existente entre a Contagem de Células Somáticas e os parâmetros de composição química presentes no leite de cabra.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Leite de Cabra; Composição Química; Caprino; Mamite; Goat milk; Goats; Chemical composition; Somatic cell count; Mastitis.
Ano: 2018
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1101514

Mastite ovina: desafio para a ovinocultura de corte.
Provedor de dados: 14
Autores: SANTANA, R. C. M.; ZAFALON, L. F.; RIBEIRO, M. G.; ESTEVES, S. N.
bitstream/item/151463/1/Circula74.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Mastite ovina; Ovino de corte; Prevenção da mastite; Mamite; Mastitis.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1058248

Mamite.
Provedor de dados: 14
Autores: VIDAL, C. E. S.; SANTOS, I. M. dos; VASCONCELOS, H. E. M.
Como evitar; Como tratar.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Mastite; Caprino; Doença animal; Mamite; Goats; Animal diseases; Mastitis; Mammary gland diseases.
Ano: 1990
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/514293

Teste de resistência antimicrobiana in vitro de Staphylococcus aureus isolados no leite de cabras com mastite.
Provedor de dados: 14
Autores: MENEZES, L. M. F.; ÂNGELO, F. F.; ALCINDO, J. F.; SÁ, D. M. A. T.; SOUZA, V. de.
Resumo: A mastite é uma das doenças mais comuns em propriedades leiteiras causando prejuízos, principalmente pela redução na produção de leite decorrente da invasão do agente infeccioso no epitélio secretor da glândula mamária. O uso de antibióticos adequados é uma importante ferramenta para o tratamento da enfermidade, sendo que deverão ser empregados de forma criteriosa, uma vez que a resistência bacteriana aos fármacos é a principal causa de falha de terapias. O presente estudo teve como objetivo avaliar o perfil de sensibilidade antimicrobiana in vitro de cepas de Staphylococcus aureus isolados de amostras de leite de cabras, obtidas a partir de casos de mastite subclínica de um rebanho do Estado de Minas Gerais e São Paulo. Verificou-se que a...
Tipo: Livro técnico (INFOTECA-E)
Palavras-chave: Ampicilina; Rifampicina; Doença Animal; Mamite; Antibiótico; Tratamento; Saúde Pública; Goat milk; Mastitis; Antibiotics; Chemical treatment.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1125037

Diagnóstico de mastite subclínica caprina por meio da detecção de Staphylococcus aureus utilizando a técnica de RT-PCR.
Provedor de dados: 14
Autores: MONTEIRO, J. P.; SOUZA, V. de.
A presente publicação descreve os procedimentos necessários para a identificação e confirmação molecular de estirpes de S. aureus causadoras de mastite subclínica, provenientes de amostras de leite de cabra, por meio da técnica de RT-PCR.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: RT-PCR; PCR.; Leite de cabra; Caprino; Teste de mamite; Staphylococcus aureus; Diagnóstico; Infecção; Doença animal; Goat milk; Mastitis; Diagnostic techniques; Animal diseases.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1047787

Avaliação do teor de cloretos e da condutividade elétrica no leite de cabra como métodos auxiliares de diagnóstico na mastite subclínica caprina.
Provedor de dados: 14
Autores: SOUZA, V. de; FACCIOLI-MARTINS, P. Y.; MOURA, J. W. F.; FERNANDES, D. R.; LIMA, A. R.
Esta publicação avalia os testes de detecção do teor de cloretos e da condutividade elétrica (CE) em relação ao isolamento de Staphylococcus spp.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Diagnosis.; Doença animal; Teste de mamite; Staphylococcus aureus; Caprino; Diagnóstico; Cloreto; Condutividade elétrica; Animal diseases; Mastitis; Goat milk; Chlorides; Electrical conductivity.
Ano: 2017
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1086974

Mastite caprina: informações para prevenção e controle da doença.
Provedor de dados: 14
Autores: SOUZA, V. de.
bitstream/item/223690/1/CNPC-2013-Arq-01.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Controle; Caprino; Mamite; Doença animal; Profilaxia; Goats; Mammary gland diseases; Mastitis; Disease control.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1010154

Contagem de células somáticas e california mastitis test no diagnóstico da mastite caprina subclínica.
Provedor de dados: 14
Autores: SILVA, E. R. da; SAUKAS, T. N.; ALVES, F. S. F.; PINHEIRO, R. R.
A mastite e uma doenca altamente prevalente em rebalnhos leiteiros de todo o mundo. Os agentes causais das mastites em caprinos sao semelhantes aqueles da doenca em bovinos, sendo os Staphylococcus coagulase positiva os mais frequentes patogenos associados a forma clinica e os Staphylococcus coagulase negativa a forma subclinica da mastite caprina. O diagnostico da mastite clinica e facil, baseado primariamente nos sinais clinicos; entretanto, o diagnostico da forma subclinica e um problema, tendo-se como metodos auxiliares os testes fisico-quimicos do leite e os baseados no aumento do conteudo celular da glandula mamaria. Por serem rapidos e de facil execucao, os testes baseados no aumento celular do leite sao muito utilizados, sendo o California Mastitis...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Diagnostic.; Caprino; Mamite; Diagnóstico; Goats; Mastitis; California mastitis test.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/514270

Etiologia, fatores de risco e aspectos clínicos da mastite ovina.
Provedor de dados: 14
Autores: SANTIAGO, L. B.; PINHEIRO, R. R.; ALVES, F. S. F.; CHAPAVAL, L.
bitstream/CNPC-2010/22751/1/doc87.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Ovino; Doença animal; Mamite; Glândula mamária; Infecção; Sheep; Animal diseases; Mammary gland diseases; Mastitis.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/658600

Controle integrado de mastite bovina.
Provedor de dados: 14
Autores: BRITO, J. R. F.; BRESSAN, M.
bitstream/item/132469/1/Livro-Controle-integrado-da-mastite-bovina.pdf
Tipo: Livro técnico (INFOTECA-E)
Palavras-chave: Mastite; Integrated control.; Controle Integrado; Leite.; Mastitis; Milk.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/592224

Diagnóstico da mastite subclínica caprina.
Provedor de dados: 14
Autores: SOUZA, V. de; COSTA, V. M. D.; FERNANDES, D. R.; LIMA, A. R.; PINHEIRO, R. R.
bitstream/item/140671/1/CNPC-2015-Cot150.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Cabrito; Caprino; Doença animal; Mamite; Diagnóstico; Goats; Mastitis; Mammary gland diseases; Animal diseases.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1039709

Detección de diferentes subtipos genéticos de Mycoplasma bovis en Argentina
Provedor de dados: 78
Autores: Estanguet,A.A.; Tamiozzo,P.J.; Tirante,L.; Chaves,J.; Raviolo,J.; Giraudo,J.; Ambrogi,A.
Mycoplasma bovis es el mycoplasma causante de mastitis más importante en vacas lecheras y ha sido diagnosticado en nuestro país así como a nivel mundial. Para realizar estudios epidemiológicos de brotes, el análisis por MLVA parece ser una herramienta adecuada para la tipificación genética del patógeno. En el presente trabajo se analizaron 7 cepas de M. bovis provenientes de 2 tambos distintos (muestras de leche de tanque de enfriado, y cuartos mamarios de vacas con y sin signos de mastitis) que habían sido previamente identificadas. Se estudiaron por PCR 4 regiones que comprenden secuencias repetidas en tándem (TR). Dentro de los loci TR analizados, sólo se encontró variabilidad en TR 52, en el cual se observaron 2 posibles alelos en muestras que...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ganado lechero; Mastitis; Mycoplasma bovis; Subtipos genéticos; MLVA.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982015000200003

Determinación de biomarcadores moleculares en leche y su posible aplicación en el diagnóstico y tratamiento de la mastitis bovina
Provedor de dados: 78
Autores: Caggiano,N; Bottini,J.M; Lorenzo Smirnoff,A; De Simone,E.A; Chiappe Barbará,M.A.
La mastitis es la patología con mayor morbilidad y mortalidad dentro de los rodeos lecheros, afectando seriamente a la producción láctea y la calidad de la leche. Es por eso que en este trabajo se evaluó la actividad de las Metaloproteasas 2 y 9, involucradas en la degradación de la matriz extracelular, en leche de vacas y vaquillonas con mastitis clínica y subclínica y en animales sanos. Se evaluaron también distintas fracciones proteicas y la actividad caseinolítica total. Las fracciones proteicas se evaluaron mediante SDS-PAGE y a actividad de las Metaloproteasas 2 y 9 fue evaluada mediante zimografía al igual que la actividad caseinolítica total. Los resultados arrojaron en la actividad caseinolítica total diferencias significativas entre el grupo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Metaloproteasas; Caseína.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982016000200004

Valores predictivos y sensibilidad a nivel de rodeo de mastitis a partir de las características de las pruebas diagnósticas individuales y el tamaño del muestreo
Provedor de dados: 78
Autores: Signorini,M.L.; Canavesio,V.R.; Neder,V.E.; Molineri,A.I.; Vitulich,C.A.; Tarabla,H.D.; Calvinho,L.F.
El objetivo del presente trabajo fue ilustrar las dificultades para interpretar los resultados de las principales pruebas diagnósticas individuales para infecciones intramamarias (IIM) y su relación con el estatus sanitario del rodeo y el tamaño del muestreo. Los datos empleados correspondieron a muestreos realizados en tambos de las provincias de Santa Fe, Córdoba y Buenos Aires (Argentina) entre mayo de 1999 y agosto de 2007, seleccionados sobre la base de presentar problemas de IIM. Los resultados refuerzan la importancia que debe darse al tipo de prueba empleada para estudios a nivel de rodeo y las consideraciones que deben realizarse para interpretar la distribución de las prevalencias de las enfermedades antes de realizar cualquier extrapolación a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Prueba diagnóstica; Sensibilidad; Especificidad; Valor predictivo.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982008000200004

DAIRY HEIFER MORBIDITY, MORTALITY, AND HEALTH MANAGEMENT FOCUSING ON PREWEANED HEIFERS
Provedor de dados: 31
Autores: Ott,   Stephen L.
The National Dairy Heifer Evaluation Project ( NDHEP ) was conducted by the National Animal Health Monitoring System (NAHMS ) from April 1991 through July 1992. A total of 921 producers monitored calves on a daily basis from the first through the eighth week of life; results were extrapolated to the U.S. dairy population. Mortality of heifer calves was examined according to region ( west, midwest, northeast, southeast ). Mortality was highest in the western region ( 9.1 percent ) and lowest in the midwestern region ( 5.6 percent ). Calf deaths were highest in the two quarters October-December and January-March ( 8.4 percent each quarter ) and lowest in the quarter April-June ( 5.2 percent ). The overall average of stillborn calves was 1.6 percent. Calving...
Tipo: Report
Palavras-chave: NAHMS; Dairy; Cattle; Monitoring; Epidemiology; Preweaning; Heifer; Production; Diseases; Morbidity; Mortality; Death; Illness; Respiratory; Reproduction; Digestive; Musculoskeletal; Nervous system; Mastitis; Maternity; Injections; Antibiotics; Milk replacer; Livestock Production/Industries.
Ano: 1993
URL: http://purl.umn.edu/32756

DAIRY '96, PART III: REFERENCE OF 1996 DAIRY HEALTH AND HEALTH MANAGEMENT
Provedor de dados: 31
Autores: Ott,   Stephen L.
The NAHMS Dairy '96 Study was designed to provide both participants and the industry with information on the nation's dairy animal population for education and research.  The USDA's National Agricultural Statistics Service (NASS) collaborated with NAHMS to select a statistically valid sample yielding 2,542 producers.  Included in the study were 20 states that represented 83.1 percent of the U.S. milk cows as of January 1, 1996.  Veterinary Medical Officers (VMO's) and Animal Health Technicians (AHT's) collected data for Part III from 1,219 operations that had 30 or more milk cows on January 1, 1996, from February 20 through May 24, 1996.   Contact for this paper: Steven Ott
Tipo: Report
Palavras-chave: NAHMS; Dairy; Cattle; Health; Mastitis; Labor; Antibiotics; Biosecurity; Vaccination; Manure management; Livestock Production/Industries.
Ano: 1996
URL: http://purl.umn.edu/32752

Incidencia y costo de la Mastitis en un establo del Municipio de Santa Ana, Sonora
Provedor de dados: 31
Autores: Gerlach B.,   Francisco Arturo; Ayala Alvarez,   Felix; Denogean Ballesteros,   Francisco G.; Moreno Medina,   Salomon; Gerlach B.,   Luis Ernesto.
With the objective to know the mastitis cost for milk producer, was developed this study, with duration of a year in a milk farm of the North region of Sonora. The cost of the clinical and subclinical mastitis was estimated, taking into account the milk production, treatment costs and commercial milk value. The information was recorded monthly, the incidence of subclinical mastitis was obtained using California MastitisTest (CMT) and clinical mastitis incidence was obtained from farm health records. Was an incidence average of 18,3% of subclinical mastitis, whereas the incidence of the clinical form of mastitis was of 5,35%. The average monthly cost of each animal with mastitis was $185.40; altogether the mastitis had a cost of $30.966,34 annually,...
Tipo: Journal Article
Palavras-chave: Milk; Mastitis; Diseases costs; Dairy cattle; Agribusiness.
Ano: 2009
URL: http://purl.umn.edu/48607

Association of the occurrence of some diseases with reproductive performance and milk production of dairy herds in southern Brazil
Provedor de dados: 96
Autores: Scheid Filho,Virgilio Balduíno; Schiavon,Raquel Schiavon; Gastal,Gustavo Desire Antunes; Timm,Cláudio Dias; Lucia Jr.,Thomaz.
This study analyzed associations between the occurrence of mastitis, metritis and retained placenta with the reproductive performance and milk production of four Holstein dairy herds of southern Brazil. Calving-to-conception intervals (CCI) and daily milk production were compared across herds, number of artificial inseminations and clinical occurrences and occurrence of mastitis, metritis and retained placenta. The CCI of cows with three or more clinical occurrences was longer than for those with no occurrences, but shorter for cows with retained placenta than for those without it. Cows with clinical mastitis up to 60 days post-partum had shorter CCI than those with mastitis after 60 days. Cows with retained placenta also had higher frequency of metritis....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dairy; Mastitis; Production; Retained placenta.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200033

Aplicación de plantas medicinales para la antisepsia de pezones de vacas posordeño
Provedor de dados: 98
Autores: Almeida Schiavon,Diane Bender; Damé Schuch,Luiz Filipe; Bastos Oyarzabal,Marta Elaine; de Souza Prestes,Luciana; Zani,João Luiz; de Andrade Hartwig,Carla.
Introducción: el sistema de producción de leche agroecológico está limitado por alternativas sostenibles de promoción a la salud animal. El control de la mastitis con plantas medicinales puede ser viable de modo económico y ecológicamente. Objetivos: evaluar la aplicación de antisépticos en la desinfección de pezones posordeño. Métodos: se comparó un convencional y extracto de plantas medicinales en un rebaño comercial y tuvo como principal encierre las nuevas infecciones intramamarias. Resultados: la prevalencia semanal del California Mastitis Test varió en el grupo 1 entre 29,5 y 17,1 %, y en el grupo 2 de 29,7 a 19,6 %, no diferenciándose de modo significativo, en ninguna de las semanas. La incidencia de cultura positiva para...
Tipo: Journal article
Palavras-chave: Mastitis; Plantas medicinales; Tagetes minuta.
Ano: 2011
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962011000300005

Actividad antimicrobiana de aceite esencial de Origanum vulgare L. ante bacterias aisladas en leche de bovino
Provedor de dados: 98
Autores: Bastos Oyarzabal,Marta Elaine; Damé Schuch,Luiz Filipe; de Souza Prestes,Luciana; Bender Almeida Schiavon,Diane; Alves Rodrigues,Maria Regina; Braga de Mello,João Roberto.
Introducción: la mastitis bovina es el mayor problema en la producción lechera, causada principalmente por bacterias grampositivas. La búsqueda de principios activos que actúen en esos microrganismos es creciente, sobre todo por la ocurrencia de multirresistencia bacteriana. Objetivos: evaluar la concentración bactericida mínima del aceite esencial de Origanum vulgare L. (orégano) frente a bacterias aisladas de leche mastítica. Métodos: se evaluó la actividad antimicrobiana del aceite esencial de O. vulgare frente a 71 bacterias aisladas de leche bovina, de los géneros Streptococcus, Staphylococcus y Corynebacterium; y 3 cepas patrón de Pseudomonas aeruginosa, Staphylococcus aureus y Escherichia coli. La técnica utilizada fue de dilución en microplaca....
Tipo: Journal article
Palavras-chave: Origanum vulgare; Mastitis; Antimicrobiano.
Ano: 2011
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962011000300006

Actividad antibacteriana de los extractos de Syzygium cumini (L.) Skeels (jambolán) frente a los microorganismos asociados a la mastitis bovina
Provedor de dados: 98
Autores: Voigt Mota,Fernanda; Damé Schuch,Luiz Filipe; Lambrecht Gonçalves,Carolina; Faccin,Ângela; Almeida Schiavon,Diane Bender; Conrad Bohm,Bianca; Ferreira Lessa,Lisiane.
Introducción: la aparición de cepas bacterianas resistentes a los antibióticos ha estimulado el desarrollo de nuevas líneas de investigación, basadas en las propiedades antibacterianas de plantas utilizadas en medicina popular. La medicina veterinaria ha estado tomando para sí los beneficios de la medicina tradicional, en un intento de sustituir el uso de antibióticos en el tratamiento y la prevención de la mastitis. Objetivo: evaluar la actividad antibacteriana de los extractos hidralcohólicos de Syzygium cumini (L.) Skeels, en comparación con las bacterias relacionadas en la mastitis bovina. Métodos: se obtuvieron extractos de hojas frescas con alcohol a 92,8° y de hojas secas con alcohol a 70°. La actividad antimicrobiana de plantas se evaluó utilizando...
Tipo: Journal article
Palavras-chave: Planta medicinal; Syzygium cumini; Extractos hidroalcohólicos; Mastitis.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962013000300016

Detección de mastitis bovina subclínica por micoplasmosis mediante ELISA indirecta y aislamiento
Provedor de dados: 108
Autores: Núñez D,César; Morales Salinas,Elizabeth; Martínez Maya,José Juan; Hernández A,Laura.
El objetivo de este trabajo fue determinar la efectividad de una prueba de ELISA en suero para el diagnóstico de la mastitis bovina subclínica causada por Mycoplasma bovis, teniendo como prueba de referencia el aislamiento del microorganismo a partir de muestras de leche. Se obtuvieron 225 muestras de sangre y de leche provenientes de hatos del Estado de México, Coahuila e Hidalgo, todos en México. Ciento treinta y nueve muestras (61.8%) resultaron positivas a mastitis subclínica mediante la prueba de Wisconsin, y de ellas sólo en seis se aisló M. bovis. A través de la prueba de ELISA, 72 muestras resultaron positivas (32.0%). Todos los animales con aislamiento positivo también fueron positivos a la prueba de ELISA. Con un punto de corte mayor o igual a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma bovis; Mastitis; Pruebas Diagnósticas; Serología.
Ano: 2008
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922008000200005

History of mastitis clinical classification
Provedor de dados: 195
Autores: Gregory, Lilian; Birgel, Eduardo Harry; Hoedemaker, Martina; Grunert, Eberhard.
This review will expose an account of the history of mastitis ranging from the 18th century until present. The bibliography was found in the library of the Tierfutzliche Hochschule (School of Veterinary Medicine) of Hannover, Germany.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Veterinary clinical practice; Bovine mastite; Clínica médica veterinária; Bovinos.
Ano: 2001
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3303

The importance of mastitis in dairy production in Brazil
Provedor de dados: 195
Autores: Costa, Elizabeth Oliveira da.
Mastitis is an inflammation of the mammary gland that causes severe economic losses due to reduced milk production, expence on pharmaceuticals, veterinary care, discarding of milk after treatment and discarding of affected animals. This inflammatory process may have distinct origins, but all cases of economic importance are caused by microorganisms. According to their origin and means of transmission, the main etiological agents were put together in two groups, contagious microorganisms (Streptococcus agalactiae, Staphylococcus sp, e Corynebacterium bovis) transmitted mainly during rnilking and environmental microorganisms (Streptococcus uberis, Enterobacteriaceae, fungos, algae of the genus Prototheca, etc.).Clinical mastitis is characterized by visible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Bovine; Mammary gland; Microorganisms; Control mastite; Bovinos; Glândula mamária; Microrganismos; Controle.
Ano: 1998
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3381

Modernization trends in the dairy industry: milk quality monitoring by somatic cell counts
Provedor de dados: 195
Autores: Langoni, Hélio.
The dairy industry suffers heavy losses as a result of mastitis, the main infection of dairy cattle. Itsmultiple etiology makes prophylaxis very difficult. The present study reflects the importance ofmastitis control, and discusses aspects of the somatic cell counts in the monitoring of the mammarygland's health, with the objective of obtaining better quality milk, with less infection risks forconsumers, because both milk and dairy products can transmit zoonosis agents.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Somatic cells; Trends; Monitoring mastites; Células somáticas; Tendências; Monitoramento.
Ano: 2000
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3332

PREVALENCIA DE MASTITIS BOVINA SUBCLÍNICA Y MICROORGANISMOS ASOCIADOS: COMPARACIÓN ENTRE ORDEÑO MANUAL Y MECÁNICO, EN PERNAMBUCO, BRASIL
Provedor de dados: 101
Autores: Ruiz,A.K; Ponce,P; Gomes,G; Mota,R.A; Elizabeth,Sampaio; Lucena,E.R; Benone,S.
Se estudiaron 11 propiedades productoras de leche bovina del estado de Pernambuco, Brasil; seis con ordeño manual y cinco con ordeño mecánico. Los rebaños estaban constituidos por animales de varias razas, edades y periodos de lactación. Se tomaron muestras de 185 vacas, resultando en 708 cuartos muestreados. Antes de colectar se realizó la prueba de California (CMT), con independencia de este resultado fueron colectadas muestras para cultivo microbiológico y conteo de células somáticas (CCS) y composición. Se obtuvieron prevalencias de 39,3% y 54,8% (CMT), 57,2% y 63,3% (diagnóstico microbiológico), 33,4% y 49,4% (CCS), para ordeño manual y mecánico respectivamente. Se aislaron microorganismos patógenos en el 61% de las muestras con mayor prevalencia para...
Tipo: Journal article
Palavras-chave: Mastitis; Ordeño manual; Ordeño mecánico; Prevalencia; Staphylococcus spp.; Corynebacterium spp.; Streptococcus spp.; Sensibilidad antibióticos.
Ano: 2011
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2011000100009

CONTROL INTERNO DE LA CALIDAD APLICADO AL CENMAST, PRODUCTO PARA DETECTAR MASTITIS
Provedor de dados: 101
Autores: Betancourt,A; Ramírez,Anledys; Navarro,Verónica; González,Daisy; López,Yanet; Linares,Anel.
El CENMAST es un diagnosticador con un principio semejante a la prueba de California, durante su producción por el Centro Nacional de Sanidad Agropecuaria, el laboratorio de control de la calidad comprueba las especificaciones del producto, con las determinaciones del principio activo, pH y densidad, cuyos resultados están bajo un programa de control interno de la calidad. Se evaluó un lote del diagnosticador como material de referencia de trabajo, para el uso como muestra de control de la calidad, también se empleó el mismo en la validación de la precisión de los ensayos en condiciones de repetibilidad y condiciones intermedias variando los analistas, día de trabajo, equipos y la calibración. Se halló una adecuada precisión entre las réplicas, se...
Tipo: Journal article
Palavras-chave: Control interno de la calidad; Diagnosticador; Mastitis.
Ano: 2010
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2010000300004

Reducción de la mastitis subclínica con el empleo de implante de catgut en el punto de acupuntura Estómago 29
Provedor de dados: 101
Autores: Farray González,Jesús Francisco; Rubio García,Juan Luís; Fabré Rodríguez,Yolexis; Alonso Rodríguez,Julio César; Suárez Fernández,Yolanda E.
Se realizó un estudio observacional de diseño transversal en la población bovina del Rancho Lupita, Aguascalientes, México, en el periodo junio-julio de 2013, con el propósito de reducir la prevalencia de mastitis e incrementar la recuperación de cuartos y animales, a través del empleo de acciones y procedimientos de prevención y control. Para determinar la prevalencia, se diagnosticó la mastitis por examen físico individual, mediante la prueba de California, por el conteo de células somáticas y por aislamiento bacteriológico. Para la reducción de la mastitis se instauraron medidas preventivas y de control de la enfermedad y se evaluó la recuperación de los cuartos y animales afectados por medio del implante de catgut en el punto acupuntural E-29. Se logró...
Tipo: Journal article
Palavras-chave: Mastitis; Implante; Vacas; Estómago 29; Mastitis; Implant; Cows; Stomach 29.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000200003

Relationship between somatic cell counts and milk production and composition in Jersey cows
Provedor de dados: 101
Autores: Ramos Garcia,Roberto; Bufon Maion,Vinicius; Molin de Almeida,Kelly; Walter de Santana,Elsa Helena; Rezende Costa,Marcela; Fagnani,Rafael; Ludovico,Agostinho.
The main components in milk from cows that are affected by mastitis can be in different amounts according to the racial characteristics. Thus, the association between the somatic cell counts (SCC) and these components may also differ in milk from Jersey cows. This study evaluated the effect of the SCC in milk from Jersey cows on milk production and its physicochemical composition. For 71 months, data from 3244 milking and 300 cows were evaluated considering the effect of the SCC on milk production (kg cow day-1), percentage of fat, protein, lactose, total solids, and nonfat solids. The probable factors associated with fluctuation in milk production based on somatic cell score, lactation number and rainfall were also evaluated by regression models. Milk...
Tipo: Journal article
Palavras-chave: Productivity; Mastitis; Lactose; Protein; Losses.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000300001

Evolution and factors influencing somatic cell count in raw milk from farms in Viçosa, state of Minas Gerais
Provedor de dados: 2
Autores: Quintão, Leonardo Cotta; Cunha, Adriano França da; Bragança, Lindomar José; Coelho, Kamila Soares; Nunes, Mariana Fonseca; Saraiva, Luís Henrique Gouvêa.
The somatic cell count (SCC) of refrigerated raw milk reflects the occurrence of mastitis in the herd and the management of rural property. Therefore, this study aimed to evaluate the SCC of the refrigerated raw milk and its determinant factors in dairy properties of Viçosa, State of Minas Gerais. Monthly, milk samples were taken from 44 cooling tanks during 2012. A structured questionnaire was applied to evaluate the production and management characteristics of the herds. There was a significant variation (p &lt; 0.05) in the mean SCC of the milk, which was lower from June to August. Higher mean SCC and percentages of samples above the limit set by the legislation were observed in months of higher rainfall and ambient temperature (p &lt; 0.05). Mechanical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.05.05.00-9 environment; SCC; Mastitis; Milking; Seasonality.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/35364

Relationship between milk production system and milk traits and somatic cell counts in Brazilian Murrah buffaloes: a multivariate analysis
Provedor de dados: 2
Autores: Barbosa, Severino Benone Paes; Modesto, Elisa Cristina; Lopes, Fabiana de Araújo; Silva, Elizabete Cristina da; Abad, Atzel Cândido Acosta.
The purpose of this study was to evaluate the monthly milk production and quality of buffaloes from two milk production systems in the Brazilian northeast using the multivariate analysis: principal component analysis (PCA). A total of 2,506 individual milk recordings were performed in two production systems, containing information on milk production (kg day-1), fat, protein, lactose and total solids counts and somatic cell count (SCC). There were positive correlations between the fat content and the contents of total solids (TS) and protein, and of TS and protein. From the PCA, two main components (PC1 and PC2) were identified, explaining 67.71% of the total variation. The fat, protein, lactose and ST level, represented by PC1, explain 46.18% of the total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bubalus bubalis; Mastitis; Milk recording; Score of somatic cells.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/46522

Influence seasons of the year and somatic cell counts on physical-chemical and sensory parameters of milk and coalho cheese
Provedor de dados: 2
Autores: Lima, Edine Roberta de; Oliveira, Juliana Paula Felipe de; Bezerra, Maria de Fátima; Borba, Luis Henrique Fernandes; Bezerra, Joadilza da Silva; Rangel, Adriano Henrique do Nascimento.
 The objective of this study was to evaluate the influence seasons of the year and somatic cell counts (SCC) on physical-chemical and sensory parameters of cow’s milk from individual and collective refrigeration tanks with different milking systems. In addition to analyzing the sensory acceptance of pasteurized milk and coalho cheese developed with high (&gt; 800,000 cells mL-1) and low SCC levels (&lt;200,000 cells mL-1). Seasonality did not affect fat content, casein, lactose, total solids, defatted dry extract and SCC, but lower protein levels than May and June were observed from July to September. Based on the SCC results, milk from refrigeration tanks was classified as low SCC and high SCC. The samples were used to obtain pasteurized milk and coalho...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Qualidade do leite; Análise sensorial dairy products; Mastitis; Milk composition; Milk quality; Refrigeration tank; Shelf life.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/44666

APLICACIÓN DE UN MODELO MÚLTIPLE DE CURVA DE LACTANCIA PARA VACAS LECHERAS
Provedor de dados: 113
Autores: Pedraza G.,Carlos; Mansilla M.,Alberto; Jahn B.,Ernesto; Vidal V.,Agustín; Fajardo R.,Paola; Agüero E.,Hernán.
El objetivo del presente trabajo fue probar una nueva expresión matemática destinada a mejorar la interpretación de los recuentos de células somáticas y niveles de producción de leche. Un modelo múltiple de curva de lactancia se comparó con el modelo clásico de Wood, separadamente para cada uno de los rangos celulares (RC). Se trabajó con una base de datos depurada proveniente del control sistemático de cinco rebaños lecheros que representan una masa de 1200 vacas Holstein Friesian. Se ajustó una curva de producción de leche, separadamente para vaquillas y vacas, mediante la expresión y(t) = a t b e- c t. El modelo múltiple propuesto estuvo basado en la ecuación anterior, incorporando la variable RC en la forma y (t, RC = a t b e- c t e d RC + f RC2. El...
Tipo: Journal article
Palavras-chave: Modelo matemático; Células somáticas; Producción láctea; Mastitis.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072002000400003

Modified E-test by the addition of EDTA-Tris and dimethyl sulfoxide on the potentiation of the effects of some antimicrobials in Pseudomonas aeruginosa strains isolated from bovine mastitis
Provedor de dados: 45
Autores: Ribeiro,M.G.; Siqueira,A.K.; Langoni,H.; Paes,A.C.; Victória,C.; Silva,A.V. Da; Listoni,F.J.P.; Souza,A.H.
A concentração inibitória mínima-MIC em 30 estirpes de Pseudomonas aeruginosa isoladas de mastite bovina foi avaliada utilizando o E-test padrão e o método modificado, pela adição de Tris-EDTA e DMSO. Os métodos modificados apresentaram redução significativa da MIC das estirpes utilizando a gentamicina, a ciprofloxacina e a norfloxacina.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Mastitis; Pseudomonas aeruginosa; Minimal inhibitory concentration.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000500017

Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification
Provedor de dados: 45
Autores: Coimbra-e-Souza,V.; Brito,M.A.V.P.; Chamon,R.C.; Laport,M.S.; Giambiagi-deMarval,M.
ABSTRACT Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffalo; Mastitis; Staphylococcus; Milk; Coagulase test.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601669

TSST-1, enterotoxin and bacteriocin-like substance production by Staphylococcus aureus isolated from foods
Provedor de dados: 45
Autores: Carvalho,S.A.; Carmo,L.S.; Abreu,E.F.; Dias,R.S.; Apolônio,A.C.M.; Santos,S.G.; Farias,L.M.; Carvalho,M.A.R.
The production of Toxic Shock Syndrome Toxin-1 (TSST-1), enterotoxins and bacteriocin-like substances was evaluated in 95 strains of Staphylococcus aureus recovered from raw bovine milk (n=31) and from food samples involved in staphylococcal food poisoning (n=64). Enterotoxigenicity tests with the membrane over agar associated to optimal sensibility plate assays were performed and showed that 96.77% of strains recovered from milk and 95.31% from food samples produced enterotoxins A, B, C, D or TSST-1. Reference strains S. epidermidis, Bacillus cereus, Listeria monocytogenes, Lactobacillus casei, Pseudomonas aeruginosa, S. aureus, Salmonella Typhimurium, Escherichia coli, Enterococcus faecalis and Bacteroides fragilis were used as indicator bacteria in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Mastitis; Toxigenicity; Food poisoning; Bacteriocin.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500035

Clinical features of caprine arthritis-encephalitis infections
Provedor de dados: 45
Autores: Lara,M.C.C.S.H; Birgel Junior,E.H.; Gregory,L.; Birgel,E.H.
In a clinical survey of caprine arthritis-encephalitis in 14 naturally infected herds in the State of São Paulo, Brazil, 17.1% (64/374) of seroreagents presented the articular form of the disease and 6.6% (17/249) of the seroreagent females presented the mammary form of the disease.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprine; Arthritis- encephalitis; Mastitis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600005

Surto de mastite bovina causada por Arcanobacterium pyogenes
Provedor de dados: 45
Autores: Motta,R.G.; Ribeiro,M.G.; Perrotti,I.B.M.; Motta,D.G.; Domingues,P.F.; Lucas,T.M.; Zamprogna,T.O.; Listoni,F.J.P.
An uncommon outbreak of mastitis caused by Arcanobacterium pyogenes in 26 cows is reported. The epidemiological findings, clinical signs, microbiological exams, somatic cell count, in vitro susceptibility profile of strains, efficacy of intramammary treatment and control measures were discussed. Florfenicol (96.2%), cefoperazona (92.3%), cefaloxin (84.6%) and ceftiofur (84.6%) were the most effective antimicrobials, and neomicin (27.0%) and enrofloxacin (17.4%) the least effective antimicrobials.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cattle; Arcanobacterium pyogenes; Outbreak; Mastitis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300027

Conventional and Doppler ultrasonography on a goat with gangrenous mastitis
Provedor de dados: 45
Autores: Santos,V.J.C.; Simplício,K.M.M.G.; Sanchez,D.C.C.; Almeida,V.T.; Teixeira,P.P.M.; Coutinho,L.N.; Rodrigues,L.F.S.; Oliveira,M.E.F.; Feliciano,M.A.R.; Vicente,W.R.R.
Objetivou-se descrever os achados clínicos e ultrassonográficos de uma cabra leiteira apresentando mastite gangrenosa. Com a ultrassonografia convencional, observaram-se alterações consistentes com o avanço do quadro clínico, como alterações na ecogenicidade do leite e parênquima mamário. A ultrassonografia Doppler revelou aumento da velocidade de pico sistólico nos três momentos estudados, passando de 15 para 17 e 29cm/s. Houve redução, também, dos valores do índice de resistência, de 0,71 para 0,61 e 0,43. O índice de pulsatividade sofreu redução entre o segundo e o terceiro dia de observação, passando de 0,98 para 0,71. Foi possível estabelecer relação direta entre os achados ultrassonográficos e a gravidade da doença. A ultrassonografia convencional e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goats; Mastitis; Ultrasonography; B mode; Doppler.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601931

Mammary botryomycosis: two concomitant cases in mares: case report
Provedor de dados: 45
Autores: Belli,C.B.; Fonte,J.S.; Torres,L.N.; Gondin,M.R.; Maiorka,P.C.; Fernandes,C.B.
ABSTRACT Mammary disorders in mares are rare and usually only one animal per paddock is affected. In this report, two mares with 7 and 9 years old, were concomitantly diagnosis of chronic pyogranulomatous mastitis, associated with the Splendore-Hoeppli reaction, indicative of botryomycosis a rare, chronic suppurative disease with microabscess formation, characterized by in vivo formation of eosinophilic materials around microorganisms or biologically inert material. Various bacteria can cause botryomycosis in horses, and the most frequently isolated one is Staphylococcus sp., particularly S. aureus. This report confirms the role of Staphylococcus sp.; however, specifically S. hyicus and S. cohnii that prior to the current report, had not been associated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine; Mastitis; Mammary gland; Botryomycosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200342

Lactation stage and udder health status of Santa Ines ewes
Provedor de dados: 45
Autores: Blagitz,M.G.; Benites,N.R.; Melville,P.A.; Batista,C.F.; Betiol,P.S.; Azedo,M.R.; Gomes,V.; Souza,F.N.; Della Libera,A.M.M.P.
Avaliou-se o risco de infecção em diferentes fases da lactação em 33 ovelhas da raça Santa Inês. Inicialmente a glândula foi submetida ao exame físico e à prova de fundo escuro. Posteriormente, amostras de leite foram coletadas assepticamente para a realização de exame bacteriológico - California Mastitis teste -, e contagens microscópica e automática de células somáticas. Nenhuma diferença foi observada entre as distintas fases de lactação. Observou-se alta persistência de infecções intramamárias, e tendência a maior contagem de células somáticas no último período de lactação, que pode ser oriunda da maior resistência a infecções neste período.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ewe; Somatic cell count; Lactation stage; Mastitis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200034

Bovine mastitis in the metropolitan area of Curitiba: antibiotic resistance and antimicrobial control of the infection
Provedor de dados: 52
Autores: Parada,José Luis; Gonçalves,Dicezar; Soccol,Vanete Tomaz; Lima,Marisa; Soccol,Carlos Ricardo.
A study from cows with mastitis was performed and Staphylococcus aureus was the predominant pathogen in 46.4 % among 153 studied strains from 276 milk samples of infected cows. Antibiotic resistance of 71 S. aureus isolates was determined in order to search resistant strains to antibiotics of clinical interest, as well as to determine their degree of multi-resistance. It was found that 60% of the S. aureus strains presented resistance to β-lactams, but none to oxacillin, teicoplamin or vancomycin. On the other hand, with the aim of reducing the use of current antibiotics and their associated resistance, a new formulation was introduced. The antimicrobial compounds (P22-P32), demonstrated to be effective in 55% of the 76 mastitis cases studied. The use of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Staphylococcus aureus; Antibiotic resistance; Infection reduction.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400009

Comparative analysis of agr groups and virulence genes among subclinical and clinical mastitis Staphylococcus aureus isolates from sheep flocks of the Northeast of Brazil
Provedor de dados: 58
Autores: Almeida,Lara M. de; Almeida,Mayra Zilta P.R.B. de; Mendonça,Carla L. de; Mamizuka,Elsa M.
Staphylococcus aureus is one of the most frequent mastitis causative agents in small ruminants. The expression of most virulence genes of S. aureus is controlled by an accessory gene regulator (agr)locus. This study aimed to ascertain the prevalence of the different agr groups and to evaluate the occurrence of encoding genes for cytotoxin, adhesins and toxins with superantigen activity in S. aureus isolates from milk of ewes with clinical and subclinical mastitis in sheep flocks raised for meat production The agr groups I and II were identified in both cases of clinical and subclinical mastitis. Neither the arg groups III and IV nor negative agr were found. The presence of cflA gene was identified in 100% of the isolates. The frequency of hla and lukE-D...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Agr groups; Virulence genes; Mastitis; Sheep flock.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200026

Biochemical characteristics of typical and atypical Staphylococcus aureus in mastitic milk and environmental samples of Brazilian dairy farms
Provedor de dados: 58
Autores: Silva,Wladimir Padilha da; Destro,Maria Teresa; Landgraf,Mariza; Franco,Bernadette D.G.M.
This study reports results on the biochemical characteristics of 274 Staphylococcus aureus strains isolated from mastitic milk, hands of milkmen and milking machines. Features included colony morphology on Baird Parker agar (BPA), catalase, coagulase and thermonuclease tests. API-Staph (bioMérieux, France) was used as reference identification system. 39.4% of the colonies on BPA identified as S.aureus were atypical. They were more frequent in milk samples (94.4%). All S.aureus strains were coagulase positive but intensity of the test varied according to the source: environmental strains were 3+ or 4+, while 17.1% of strains isolated from milk presented only 1+ or 2+ results. The majority of atypical S.aureus strains presented a 4+ coagulase result. 2.2% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Atypical S.aureus; Coagulase; Thermonuclease; API-Staph; Baird-Parker agar; Milk; Mastitis; Environmental samples.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200008

Bacteriocins: exploring alternatives to antibiotics in mastitis treatment
Provedor de dados: 58
Autores: Pieterse,Reneé; Todorov,Svetoslav D.
Mastitis is considered to be the most costly disease affecting the dairy industry. Management strategies involve the extensive use of antibiotics to treat and prevent this disease. Prophylactic dosages of antibiotics used in mastitis control programmes could select for strains with resistance to antibiotics. In addition, a strong drive towards reducing antibiotic residues in animal food products has lead to research in finding alternative antimicrobial agents. In this review we have focus on the pathogenesis of the mastitis in dairy cows, existing antibiotic treatments and possible alternative for application of bacteriocins from lactic acid bacteria in the treatment and prevention of this disease.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Antibiotic; Milk; Bacteriocin; Food safety.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300003

Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis
Provedor de dados: 58
Autores: Ciftci,Alper; Findik,Arzu; Onuk,Ertan Emek; Savasan,Serap.
This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; IcaA; IcaD; Slime; Mastitis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200009

Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins
Provedor de dados: 58
Autores: Mesquita,Adriano Queiroz de; Rezende,Cintia Silva Minafra e; Mesquita,Albenones José de; Jardim,Eurione Antonio Garcia da Veiga; Kipnis,Ana Paula Junqueira.
The identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens, and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition. Twenty percent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TLR-4; Single nucleotide polymorphism; Mastitis; Milk quality.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200034

Occurrence of Staphylococcus aureus in raw milk produced in dairy farms in São Paulo state, Brazil
Provedor de dados: 58
Autores: Fagundes,Helena; Barchesi,Luciana; Nader Filho,Antonio; Ferreira,Luciano Menezes; Oliveira,Carlos Augusto Fernandes.
The objective of the present study was to evaluate the occurrence of Staphylococcus aureus in milk produced in 37 farms located in the regions of Ribeirão Preto and São Carlos, state of São Paulo, Brazil. Two-hundred and eight samples of milk from individual cows showing subclinical mastitis, and 37 samples of bulk tank milk were analyzed. S. aureus strains were detected in 18 (7.3%) milk samples: 14 (6.7%) from samples of individual cows, and 4 (10.8%) from bulk tank milk. Two individual milk samples (14.3%) and two bulk milk samples contained enterotoxigenic S. aureus. PFGE analysis revealed the genetic heterogeneity of the strains isolated from raw milk, which presented to 13 S. aureus patterns. Results confirmed the potential transmission of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Raw milk; Bovine; Mastitis; Enterotoxin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200018

Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
Provedor de dados: 58
Autores: Martins,Katheryne Benini; Faccioli-Martins,Patricia Yoshida; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Fernandes,Simone; Oliveira,Aline A.; Dantas,Ariane; Zafalon,Luiz Francisco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Sheep; Staphylococcus aureus; Antibiotic resistance; Virulence factors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535

Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis
Provedor de dados: 58
Autores: Marques,Viviane Figueira; Motta,Cássia Couto da; Soares,Bianca da Silva; Melo,Dayanne Araújo de; Coelho,Shana de Mattos de Oliveira; Coelho,Irene da Silva; Barbosa,Helene Santos; Souza,Miliane Moreira Soares de.
Abstract Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation. Biofilm production in microplates was evaluated considering the intervals determined along the bacterial growth curve. In addition, to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm; Agr types; Antimicrobial resistance; Mastitis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100118

Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms
Provedor de dados: 58
Autores: Souza,Viviane; Nader Filho,Antonio; Melo,Poliana de Castro; Ferraudo,Guilherme Moraes; Ferraudo,Antônio Sérgio; Conde,Sandra de Oliveira; Fogaça Junior,Flavio Augusto.
The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples' collection sites with the highest isolation frequency of Staphylococcus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Staphylococcus aureus; Bulk tank; PCR; PFGE.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400050

Direct identification of bovine mastitis pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in pre-incubated milk
Provedor de dados: 58
Autores: Barreiro,Juliana R.; Gonçalves,Juliano L.; Grenfell,Rafaella; Leite,Renata F.; Juliano,Luiz; Santos,Marcos V.
ABSTRACT The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were screened based on culture-positive results obtained by microbiological culture (reference method) with the following numbers of quarters positive per cow: 4 cows with 1, 8 cows with 2, 12 cows with 3 and 16 cows with 4 infected quarters per cow. For direct identification method, quarter milk samples (n = 120) were skimmed by centrifugation (10,000 × g/10 min) and pre-incubated at 37 ºC for 12 h. After pre-incubation, quarter milk samples were submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Milk; Bacteria; Mastitis; Mass spectrometry.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400801

Mammary adenocarcinoma in a mare
Provedor de dados: 65
Autores: Brito,Marilene de Farias; Seppa,Gilberto Santos; Teixeira,Luisa Gouvêa; Rocha,Thais Gomes; França,Ticiana do Nascimento; Hess,Tanja Maria; Peixoto,Paulo Vargas.
A non-lactating 17-year-old grey barren Mangalarga Marchador mare was referred to the Large Animal Veterinary Hospital of the Federal Rural University of Rio de Janeiro (UFRRJ), presenting enlargement of the mammary gland. The owner reported that the animal had a lesion in the mammary gland for at least two years, diagnosed and treated as chronic mastitis. Initially only the right gland was involved, presenting ulceration and exudation on the cutaneous surface. After 20 months, the left gland also became affected. The diagnosis of tubulo-papillary adenocarcinoma was based on the typical microscopic lesions. This work shows the importance of the histopathological examination in the differential diagnosis between the neoplasms and the chronic inflammation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine diseases; Mammary neoplasms; Mastitis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000200045

A SURVEY OF SHEEP MASTITIS IN SOUTHERN BRAZIL
Provedor de dados: 65
Autores: Vaz,Adil Knackfuss; Jones,James Eirug Thomas.
SUMMARY Ovine mastitis causes varying economic fosses in all countries where sheep are kept. A survey of 3128 breeding ewes in 22 farms in two areas with different management practices in Southern Brazil showed that the average prevalence of subclinical and chronic mastitis was 14.1% of ewes with at least one affected gland. From those, 4.49% were also bacteriologically positive. The prevalence in individual farms varied from 0 to 37% of ewes with clinical abnormalities of the udder. The overall prevalence was greater in Area II, where meat breeds predominate and animals are housed at night. It is concluded that subclinical and chronic mastitis is uniikely to be an economic problem in Area I, where wool production is the objective of the sheep industry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sheep; Ewes; Mastitis; Lambs; Brazil.
Ano: 1994
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000200022

Accurate identification of atypical Staphylococcus chromogenes plasma-clotting strains causing bovine mastitis
Provedor de dados: 65
Autores: Avellar-Costa,Pedro; Santos,Danielle Cabral dos; Lange,Carla Christine; Laport,Marinella Silva; Brito,Maria Aparecida Vasconcelos Paiva; Rossi,Ciro César; Giambiagi-deMarval,Marcia.
ABSTRACT:  We compared the potential of routine techniques used for the identification of Staphylococcus species, aiming to evaluate their accuracy in the detection of 43 Staphylococcus chromogenes strains isolated from bovine mastitis that, despite being a coagulase-negative species, are able to clot plasma. These strains could be mistakenly suspected to be S. aureus and lead to an unappropriated treatment of the disease. MALDI-TOF, PCR-RFLP of the chaperonine gene groEL, and sequencing of the 16S rRNA and elongation factor Tu gene tuf were employed. Results from the four methods were coincident for only half of the strains because of the low accuracy of the groEL PCR-RFLP (51.2% accuracy). Even though all the sequencing results were identical, the high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus chromogenes; Mastitis; MALDI-TOF; Tuf; GroEL.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600452