Consulta: subjectFacets:"Metabolism"
Registros recuperados: 176
Data/hora: 08/06/2024 03:38:40

Advances in improving mammalian cells metabolism for recombinant protein production
Provedor de dados: 69
Autores: Altamirano,Claudia; Berrios,Julio; Vergara,Mauricio; Becerra,Silvana.
Background: The production of recombinant proteins for therapeutic use represents a great impact on the biotechnology industry. In this context, established mammalian cell lines, especially CHO cells, have become a standard system for the production of such proteins. Their ability to properly configure and excrete proteins in functional form is an enormous advantage which should be contrasted with their inherent technological limitations. These cell systems exhibit a metabolic behaviour associated with elevated cell proliferation which involves a high consumption of glucose and glutamine, resulting in the rapid depletion of these nutrients in the medium and the accumulation of ammonium and lactate. Both phenomena contribute to the limitation of cell...
Tipo: Journal article
Palavras-chave: Aerobic glycolysis; Cell engineering; CHO cells; Glutaminolysis; Metabolism.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300010

Maternal transmission of mitochondrial diseases
Provedor de dados: 74
Autores: Chiaratti,Marcos R.; Macabelli,Carolina H.; Augusto Neto,José Djaci; Grejo,Mateus Priolo; Pandey,Anand Kumar; Perecin,Felipe; Collado,Maite del.
Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oocyte; Germline; Mitochondrial dynamics; MtDNA; Metabolism.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200308

Purine and pyrimidine nucleotide metabolism in Mollicutes
Provedor de dados: 74
Autores: Bizarro,Cristiano Valim; Schuck,Desirée Cigaran.
Several mollicute genome projects are underway, offering unique opportunities to study genes and metabolic pathways on a genome-wide scale. Here, we have analyzed the conservation and diversity of purine and pyrimidine metabolism in mycoplasmas. An evaluation of discrepancies between genomic analysis and enzymatic data revealed interesting aspects about these organisms. We found important examples in which enzyme activity was reported without the annotation of a corresponding gene. An interesting example concerns phosphopentomutase. In Mollicutes, we have identified CDSs orthologous to sequences recently identified as new phosphopentomutases in archaeobacteria that are structurally related to phosphomannomutases. It is suggested that these sequences could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mollicutes; Purine; Pyrimidine; Metabolism; Metabolic pathways.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200005

Gender-dependent association of HSD11B1 single nucleotide polymorphisms with glucose and HDL-C levels
Provedor de dados: 74
Autores: Turek,Luciane Viater; Leite,Neiva; Souza,Ricardo Lehtonen Rodrigues; Lima,Jovana Karoline; Milano,Gerusa Eisfeld; Timossi,Luciana da Silva; Osiecki,Ana Claudia Vecchi; Osiecki,Raul; Alle,Lupe Furtado.
In this study, we investigated the influence of two SNPs (rs846910 and rs12086634) of the HSD11B1 gene that encodes 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1), the enzyme that catalyzes the conversion of cortisol to cortisone, on variables associated with obesity and metabolic syndrome in 215 individuals of both sexes from southern Brazil. The HSD11B1 gene variants were genotyped using the TaqMan SNP genotyping assay. Glucose, triglycerides, total cholesterol, HDL-cholesterol and LDL-cholesterol were measured by standard automated methods. Significant results were found in women, with carriers of the G allele of SNP rs12086634 having higher glucose levels than non-carriers. Carriers of the A allele of SNP rs846910 had higher levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HSD11B1 gene; Men; Metabolism; Metabolic syndrome; Women.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400003

Metabolic adjustments in Satanoperca aff. jurupari (Perciformes: Cichlidae)
Provedor de dados: 74
Autores: Chippari-Gomes,Adriana R.; Leitão,Marco Aurélio B.; Paula-Silva,Maria de Nazaré; Mesquita-Saad,Lenise S. B. de; Almeida-Val,Vera Maria F.
In this paper, we describe the enzyme levels and isozyme distribution in skeletal and heart muscle of Satanoperca aff. jurupari, (Cichlidae, subgroup Geophaginae). LDH and CS were measured in skeletal and heart muscle. Starch gel electrophoresis was used to determine the isozyme/allozyme patterns in different tissues; LDH, MDHs, PGM, PGI, ADH, G-6-PDH and SOD were screened for the numbers of loci, presence of alleles, and tissue specificity. The LDH/CS ratio in heart and skeletal muscle were 173.36 and 6.1, respectively, indicating anaerobic metabolism in the former and aerobic metabolism in the latter muscle. No inhibition by pyruvate (based on the ratios of LDH activity with 1 mM and 10 mM pyruvate) was detected in heart and skeletal muscle, indicating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cichlid; Plasticity; Metabolism; Enzymes; Amazon.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100005

Using the FORESTS and KEGG databases to investigate the metabolic network of Eucalyptus
Provedor de dados: 74
Autores: Mombach,José C.M.; Lemke,Ney; Silva,Norma M. da; Ferreira,Rejane A.; Isaia Filho,Eduardo; Barcellos,Cláudia K.; Ormazabal,Rodrigo J.
In this work we apply a bioinformatics approach to determine the most important enzymes of the metabolic network of Eucalyptus to determine the coverage of the genome in the FORESTS library. We conclude that the library does not cover completely the metabolism of the organism. However, some important pathways could be analyzed, especially the lignin synthesis. We found that four of the most important enzymes predicted are involved in this pathway.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Eucalyptus; Metabolism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400018

Genome-wide comparison of genes involved in the biosynthesis, metabolism, and signaling of juvenile hormone between silkworm and other insects
Provedor de dados: 74
Autores: Cheng,Daojun; Meng,Meng; Peng,Jian; Qian,Wenliang; Kang,Lixia; Xia,Qingyou.
Juvenile hormone (JH) contributes to the regulation of larval molting and metamorphosis in insects. Herein, we comprehensively identified 55 genes involved in JH biosynthesis, metabolism and signaling in the silkworm (Bombyx mori) as well as 35 in Drosophila melanogaster, 35 in Anopheles gambiae, 36 in Apis mellifera, 47 in Tribolium castaneum, and 44 in Danaus plexippus. Comparative analysis showed that each gene involved in the early steps of the mevalonate (MVA) pathway, in the neuropeptide regulation of JH biosynthesis, or in JH signaling is a single copy in B. mori and other surveyed insects, indicating that these JH-related pathways or steps are likely conserved in all surveyed insects. However, each gene participating in the isoprenoid branch of JH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juvenile hormone; Biosynthesis; Metabolism; Signaling; Gene duplication.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300018

Differential metabolism of Mycoplasma species as revealed by their genomes
Provedor de dados: 74
Autores: Arraes,Fabricio B.M.; Carvalho,Maria José A. de; Maranhão,Andrea Q.; Brígido,Marcelo M.; Pedrosa,Fábio O.; Felipe,Maria Sueli S.
The annotation and comparative analyses of the genomes of Mycoplasma synoviae and Mycoplasma hyopneumonie, as well as of other Mollicutes (a group of bacteria devoid of a rigid cell wall), has set the grounds for a global understanding of their metabolism and infection mechanisms. According to the annotation data, M. synoviae and M. hyopneumoniae are able to perform glycolytic metabolism, but do not possess the enzymatic machinery for citrate and glyoxylate cycles, gluconeogenesis and the pentose phosphate pathway. Both can synthesize ATP by lactic fermentation, but only M. synoviae can convert acetaldehyde to acetate. Also, our genome analysis revealed that M. synoviae and M. hyopneumoniae are not expected to synthesize polysaccharides, but they can take...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mollicutes; Mycoplasma; Metabolism; Comparative genomics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200004

Acephate and methamidophos residues in greenhouse and in field grown tomatoes
Provedor de dados: 75
Autores: Trevizan,Luiz Roberto P.; Baptista,Gilberto C. de; Papa,Geraldo.
Increasing horticultural production under protected cultivation requires more detailed studies about the occurence of pesticide residues, due to the special and peculiar environment under controlled conditions, in which some pests, that are of little concern in the field, become important. The insecticide acephate and its methamidophos metabolite residues were evaluated in greenhouse-grown tomatoes and compared to an open-field tomato crop. The treatments: (a) check; (b) one application of 75 g a.i. acephate.100 L-1 water; (c) one application of 150 g a.i.100 L-1 water; (d) four applications of 75 g a.i.100 L-1 water were evaluated. Fruit, leaf and soil samples were taken at (-1), zero, 1, 3, 7, 14, 21 days after the last or only application. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycopersicon esculentum; Pesticide residues; Metabolism; Greenhouse.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362005000100008

Энергетический баланс и чистая эффективность роста плоской устрицы (Ostrea edulis L.)
Provedor de dados: 13
Autores: Золотницкий, А. П.; Сытник, Н. А.
Исследованы энергетический баланс и чистая эффективность роста (коэффициент К2) плоской устрицы (Ostrea edulis L.) в онтогенезе. Выявлены общие тенденции изменений отдельных его компонентов в процессе роста и распределение энергетических трат на индивидуальную продукцию, энергетический обмен, ассимилированную и потреблённую пищу. Дана характеристика сезонных изменений чистой эффективности роста, связанных с температурой воды и массой тела, динамика которых описывается уравнением множественной регрессии. Показано, что максимальные значения К2 у исследованного вида могут составлять 0.63 – 0.67.
Tipo: Journal Contribution
Palavras-chave: Устрица; Энергетический баланс; Рост; Метаболизм; Ассимиляция; Рацион; Эффективность; Устриця; Енергетичний баланс; Зростання; Метаболізм; Асиміляція; Раціон; Ефективність; Oyster; Energy balance; Growth; Metabolism; Assimilation; Ration; Efficiency.
Ano: 2010
URL: http://repository.ibss.org.ua/dspace/handle/99011/1919

Seasonal variation of the effect of high-carbohydrate and high-protein diets on the intermediate metabolism of Parastacus brasiliensis (Crustacea, Decapoda, Parastacidae) maintained in the laboratory
Provedor de dados: 76
Autores: Dutra,Bibiana K.; Silva,Karina M. da; Zank,Caroline; Conter,Maria R.; Oliveira,Guendalina T.
The goal of this study was to evaluate the effect of a high-carbohydrate diet (HC) and a high-protein diet (HP) on the metabolism of the crayfish Parastacus brasiliensis (Von Martens, 1869), collected in different seasons and maintained in the laboratory for 15 days. Crayfish were collected monthly from January 2002 to January 2004 at São Francisco de Paula, Southern Brazil, in Guarapirá stream. In the laboratory, the animals were kept submerged in aquariums under controlled conditions. They were fed ad libitum, for 15 days with either a HC or HP diet. At the end of this period, haemolymph samples were collected, as were hepatopancreas, gills, and abdominal muscle that were removed for determination of glycogen, free glucose, lipids, and triglycerides. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crayfish; Experimental culture; Diet; Metabolism; Seasonality.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212008000400003

Physiological responses of anti-predation in prey fish to the threat of piscivorous fish in different underwater visibility conditions
Provedor de dados: 76
Autores: Fiori,Leandro Fabrício; Figueiredo,Bruno R. S.; Pavanello,Audrei; Alves,Vander Silva; Mathias,Paulo Cezar de Freitas; Benedito,Evanilde.
ABSTRACT Considering that the anti-predation behaviour of prey fishes may vary with predator hunting strategy, we experimentally investigated the physiological responses of Astyanax bimaculatus (Linnaeus, 1758) shoals to the presence of two piscivorous fish species with different hunting modes: sit-and-wait and active pursuit. In addition, we evaluated the influence of underwater visibility conditions on the interaction between predator and prey. We tested the hypotheses that: (i) prey plasma cortisol and glucose levels vary according to piscivore hunting strategy, and (ii) no reduction in plasma glucose and cortisol levels takes place in turbid environments due to the inability of prey to accurately recognise predators by non-visual signals. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolism; Hoplias aff. malabaricus; Astronotus crassipinnis; Astyanax bimaculatus; Turbidity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212018000100232

Transferência de supressividade de solos a Rhizoctonia solani e efeito de metabolismos voláteis.
Provedor de dados: 14
Autores: GHINI, R.
A possibilidade de transferencia da supressividade a R. solani GA 4 foi testada atraves da mistura de um solo supressivo com um solo conducente nas concentracoes de 0; 0,1; 0,5; 1; 5; 10 e 100%. O aumento da concentracao do solo supressivo resultou numa reducao do crescimento micelial do patogeno. No houve transferencia da supressividade apos o tratamento biocida do solo (fumigacao com cloroformio), comprovando o efeito dos microrganismos na supressividade. A presenca de metabolitos volateis foi outro mecanismo de supressividade observado.
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Solo supressivo; Metabolismo; Queima da Saia; Rhizoctonia Solani; Solo; Metabolism; Soil.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1111024

Defasagem entre as produtividades real e potencial do algodoeiro herbáceo: limitações morfológicas, fisiológicas e ambientais.
Provedor de dados: 14
Autores: BELTRÃO, N. E. de M.; AZEVEDO, D. M. P. de.
Fatores limitantes relacionados com a morfologia, a organografia e arquiteture do algodoeiro herbaceo; fatores limitantes relacionados com -a fisiologia e a bioquimica da planta do algodoeiro herbaceo; fatores limitantes relacionados com as condicoes do ambiente; consideracoes holoticas envolvendo fatores da planta e do ambiente.
Tipo: Livro técnico (INFOTECA-E)
Palavras-chave: Herbaceo; Taxas de Crescimento; Cultura; Fotorrespiracao; Trofofilos; Dossel; Balanco; Assimilado; Limitacao incontrolavel; Desenvolvimento; Brasil; Paraiba; Annual cotton; Production; Growth rate; Reespiration; Assimilation; Crop; Levll; Not controllable; Limitation; Growth; Deelopment.; Algodão; Bioquímica; Crescimento; Fotossíntese; Fisiologia Vegetal; Gossypium Hirsutum; Metabolismo; Respiração.; Produção; Biochemistry; Canopy; Leaves; Physiology.; Photosynthesis; Photorespiration; Metabolism.
Ano: 1993
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/264934

Ação hormonal da leptina em ruminantes.
Provedor de dados: 14
Autores: SALMAN, A. K. D.; COSTA, R. B.
A leptina, chamada de hormônio da obesidade, é produzida e secretada pelos adipócitos. É um hormônio capaz de alterar o consumo de alimento e o gasto de energia, por parte do animal, pois informa o cérebro sobre a quantidade de energia armazenada em forma de gordura. Propõe-se, nesta Documentos, explicar a ação da leptina em ruminantes. O trabalho apresenta os seguintes tópicos: Polimorfismos no gene da leptina; Expressão gênica da leptina; Os receptores da leptina; Controle do apetite pela leptina; Modulação fisiológica da leptina; Ação da leptina sobre a reprodução.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Leptina; Animal hormone.; Bovino; Hormônio Animal.; Metabolismo Animal; Metabolism; Cattle; Leptin.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/710735

The planktonic stages of the salmon louse ( Lepeophtheirus salmonis) are tolerant of end-of-century p CO2 concentrations
Provedor de dados: 5
Autores: Thompson, Cameron R.s.; Fields, David M.; Bjelland, Reidun M.; Chan, Vera Bin San; Durif, Caroline M.f.; Mount, Andrew; Runge, Jeffrey A.; Shema, Steven D.; Skiftesvik, Anne Berit; Browman, Howard I.
The copepod Lepeophtheirus salmonis is an obligate ectoparasite of salmonids. Salmon lice are major pests in salmon aquaculture and due to its economic impact Lepeoph- theirus salmonis is one of the most well studied species of marine parasite. However, there is limited understanding of how increased concentration of pCO2 associated with ocean acidification will impact host-parasite relationships. We investigated the effects of increased pCO2 on growth and metabolic rates in the planktonic stages, rearing L. salmonis from eggs to 12 days post hatch copepodids under three treatment levels: Control (416 matm), Mid (747 matm), and High (942 matm). The pCO2 treatment had a significant effect on oxygen consumption rate with the High treatment animals exhibiting...
Tipo: Text
Palavras-chave: Salmon lice; Copepod; Ocean acidification; Parasite; Energetics; Metabolism; Growth; Lipid; Lepeophtheirus salmonis; Aquaculture.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00588/69998/67911.pdf

Effets d’une alimentation végétale sur la biosynthèse des acides gras et le métabolisme du bar européen (Dicentrarchus labrax)
Provedor de dados: 5
Autores: Geay, Florian.
In the context of the fish farming industry needing to substitute the animal food (meal and oil) by vegetable ingredients, the main objective of this thesis was to study the long-term effects of a diet exclusively composed by plant products on the metabolism of the european sea bass (Dicentrarchus labrax) and more particularly, on its capacity to biosynthesis the Highly Unsaturated Fatty Acids (HUFA) from the PolyUnsaturated Fatty acids (PUFA) brought by this vegetal diet. Our transcriptomic approaches showed that the use of a plant food disrupted the lipids metabolism (cholesterol and fatty acids) and the energy metabolism (carbohydrates, amino acids) in the liver, but also affected other physiological processes such as the immune system. In spite of the...
Tipo: Text
Palavras-chave: Aquaculture; Nutrition; Métabolisme; Lipides; Désaturase; Delat-6-désaturase (D6D); Huile végétale; Farine végétale; Acides gras à longues chaînes carbonées polyinsaturés (AGLPI); Aquaculture; Nutrition; Metabolism; Lipids; Desaturase; Fatty acids desaturase 2 (fads2); Vegetable oil; Vegetable meal; Highly unsaturated fatty acids (HUFA).
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00048/15925/13352.pdf

Seasonal and diurnal variability in carbon respiration, calcification and excretion rates of the abalone Haliotis tuberculata L.
Provedor de dados: 5
Autores: Chapperon, Coraline; Clavier, Jacques; Dugué, Clément; Amice, Erwan; Le Goff, Marion; Roussel, Sabine.
Abalone (Haliotis spp.) are commercially important marine shellfish species worldwide. Knowledge about the physiology of abalone that impacts life-history traits is important for a better understanding of the biology of the species and the impact of stressful husbandry procedures at different seasons. The present study quantified the seasonal and diurnal variations in four physiological parameters of the European species Haliotis tuberculata, i.e. carbon aerial and aquatic respiration, calcification and excretion rates, and the effect of prolonged aerial exposure upon abalone aerial respiration. We also investigated the effect of individual size upon these physiological parameters. Aquatic respiration and calcification rates showed an allometric...
Tipo: Text
Palavras-chave: Ormer; Metabolism; Carbon fluxes; Physiology.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00431/54243/55886.pdf

Zooplankton metabolism in South African estuaries: does habitat type influence ecological strategies?
Provedor de dados: 5
Autores: Tagliarolo, Morgana; Porri, F; Garvie, C D; Lechman, K; Scharler, U M.
Zooplankton community composition, biomass and metabolism can vary drastically over space and time in subtropical estuaries. Changing environmental conditions can affect communities differently, depending on the characteristics of the species involved. In the present study, we compared the rates of oxygen consumption of the dominant zooplankton taxa living in permanently open and in temporally open/closed estuaries. The metabolic response was tested at four temperatures experienced by the animals in their natural environment. Zooplankton from the temporally open/closed estuary showed low activation energies and low rate of metabolism at the highest temperature tested. Animals from the permanently open estuary had higher respiration rates at increased...
Tipo: Text
Palavras-chave: Metabolism; Zooplankton; Respiration; Estuary; Thermal sensitivity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00513/62425/66904.pdf

Experimental shift in diet Delta13C: A potential tool for ecophysiological studies in marine bivalves
Provedor de dados: 5
Autores: Paulet, Yves-marie; Lorrain, Anne; Richard, Joëlle; Pouvreau, Stephane.
To test the potential of diet switching experiments in ecophysiological studies of marine invertebrates, stable carbon isotope ratios were measured at different seasons in the gonad, adductor muscle, digestive gland and gills of scallops (Pecten maximus) and oysters (Crassostrea gigas) held for 15 days on a constant diet of phytoplankton depleted in 13C. The aim of this study was to determine if differences in carbon incorporation could be detected among species, seasons and organs, and if so, whether it was consistent with their known energy-allocation patterns. After offering the new diet, isotope values of the different organs gradually shifted and significant differences among organs, seasons and species were found. A carbon incorporation index (CII)...
Tipo: Text
Palavras-chave: Crassostrea gigas; Pecten maximus; Carbon isotopes; Metabolism; Energy allocation.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1202.pdf

Etude experimentale preliminaire sur les echanges gazeux chez Pinna nobilis
Provedor de dados: 5
Autores: Vicente, N; Riva, A; Butler, A.
Breathing exchanges of Pinna nobilis L. are studied in situ and in the laboratory with two methods. First method consist in introducing samples of Pinna under a 40l plexiglass bell equipped with haps. The bell is positionned by diving. The consumption of oxygen is observed with water samples and the oxygen concentration is measured with an electronic oxymeter. Experiments which are carried out during nycthemeral cycles are reproduced in the laboratory with the same type of bell. A second method carry into effect an apparatus more sophisticated, who put into circulation a current of water in an airtight thermostated cellule, where a specimen of young Pinna is introduced. This sample is gradually adaptated to the conditions of the medium. Breathing...
Tipo: Text
Palavras-chave: Pinna nobilis; Bivalvia; Temperature; Metabolism; Oxygen consumption; Experimental research; Breathing mixtures; Marine molluscs.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/acte-1761.pdf

Production and consumption of B group vitamins in situ.
Provedor de dados: 5
Autores: Kurata, A.
The changes in concentration of thiamine, biotin and vitamin B sub(12) in water of the soaked bottles for 6 h in situ in the north basin of Lake Biwa were examined successively over 30 hours. The concentrations of the vitamins changed comparatively during 6 h. soaking in situ. Generally, the concentrations of thiamine, biotin and vitamin B sub(12) were in high levels in the morning and in low levels in evening both in the soaked bottles and in the surrounding water. The fluctuation ranges in concentration of vitamins in a 5 m deep layer were greater than those in thermocline. From the data obtained with the soaked bottles in situ, it was supposed that the decrease of vitamins in concentration during the daytime surpass consumption of vitamins by...
Tipo: Text
Palavras-chave: Phytoplankton; Microorganisms; Bacteria; Metabolism; Biological production; Vitamin B; Fresh water.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-958.pdf

Etude de la diversité métabolique des micro-organismes des sources hydrothermales
Provedor de dados: 5
Autores: Byrne, Nathalie.
Since the discovery of oceanic hydrothermal vents, microbial diversity of this extreme environment was studied using both cultivation and molecular approaches. In this study the microbial diversity of active chimney from both TAG and Lucky Strike sites (Mid Atlantic Ridge) was investigated using both molecular survey and continuous enrichment culture in a bioreactor. To reproduce as close as possible environmental conditions the cultures were performed in oligotrophic conditions, in the presence of hydrogen and carbondioxide in the gas sweeping mix or using in situ sampled fluid/sea water mix as culture medium. The effect of environmental changes such as temperature and the absence or presence of sulphur was also investigated. Archaeal and Bacterial...
Tipo: Text
Palavras-chave: Culture; Molecular diversity; Metabolism; Hydrothermal vents; Microorganisms; Microbiology; Culture; Diversité moléculaire; Métabolisme; Sources hydrothermales; Micro organismes; Microbiologie.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/these-7295.pdf

Methods for microbial biomass, community structure and metabolic activities on surfaces.
Provedor de dados: 5
Autores: White, D.
Microbes in nature are often concentrated on surfaces where they exist in microcolonies of mixed composition. The assay of these mixed microbial consortia by utilizing biochemical components eliminates the problems associated with cultural selectivity of viable counts or the requirement for quantitative release from surfaces required in some microscopic techniques. The assay of cellular components restricted to subsets of the microbial community gives insights into the community structure of the biofilm whereas assay of cellular components common to all cells can be used for biomass estimations. Formation of endogenous storage materials can be utilized as measures of nutritional status and rates of formation or turnover of specific "signatures" from...
Tipo: Text
Palavras-chave: Bacteria; Fouling; Bioassays; Metabolism; Surfaces; Bacteria collecting devices; Culture media; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-963.pdf

Substitution of fish meal with plant protein sources and energy budget for white shrimp Litopenaeus vannamei (Boone, 1931)
Provedor de dados: 5
Autores: Suarez, J. A.; Gaxiola, G.; Mendoza, R.; Cadavid, S.; Garcia, G.; Alanis, G.; Suarez, A.; Faillace, J.; Cuzon, Gerard.
Fish meal (FM) has long been considered an essential ingredient for the formulation of shrimp feeds. Until recently, 12%FM was considered a minimum amount, below which weight gain would become reduced due to a reduction in feed intake. In order to test this perceived minimum value, an experiment was conducted on a long-term basis with 4 replicates. Four practical isonitrogenous and isoenergetic diets were formulated for Litopenaeus vannamei juveniles. Survival, weight gain, feed conversion ratio (FCR) and protein efficiency ratio were determined for 0, 6, 10 and 15% FM diets and a reference diet. Shrimp survival ranged from 84 to 86.5%. Shrimp fed a diet with 0% FM showed lower average weight and SGR compared to the other treatments (P&lt;0.05). FCR of...
Tipo: Text
Palavras-chave: Metabolism; Growth; Plant proteins; Substitution fish meal; L. vannamei.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6630.pdf

Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection
Provedor de dados: 5
Autores: Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie.
Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters...
Tipo: Text
Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf

Nutrition phosphoree chez Chondrus crispus (Rhodophycees)
Provedor de dados: 5
Autores: Chopin, T; Floc'H, J; Bodeau, B; Penot, M.
Phosphorated nutrition of the red alga Chondrus crispus Stackh. is studied is two morphologically very dissimilar forms of the thallus. Phosphorus measurements, which were monthly carried out at different parts of the alga, showed first that the distribution of this element is not homogeneous along the thallus, and second that seasonal variations occur. It is suggested that the classical polymorphism of the species can be taken into account as concerns its physiology. Besides, from absorption and exsorption experiments which were performed with super(32)P as a tracer, it is shown that the high concentration factor of phosphorus in the alga as compared to the environmental seawater (up to 17.9 x 10 super(4)) can be explained as an effect of metabolic process.
Tipo: Text
Palavras-chave: Chondrus crispus; Algae; Distribution; Metabolism; Seasonal variations; Food absorption; Phosphorus; Plant nutrition.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1175.pdf

L'adaptation ecophysiologique et sanitaire de l'ecrevisse turque Astacus leptodactylus a des milieux saumatres: Contribution a l'astaciculture
Provedor de dados: 5
Autores: Barbe, D; Lautier, J; Vernet, G; Vey, A.
This influence of salt media on different aspects of biological cycle and on most common pathogenic agents of Turkish freshwater crayfish Astacus leptodactylus is investigated survival and growing of juveniles O super(+) and intermediary individuals 1 super(+) are also studied. Total osmotic metabolism and reproduction, have been recorded. It appears that youngest individuals are particularly sensitive to low salinities (7 ppt.). Intermediary ones are more resistant nevertheless salinity does not improve neither survival nor growing. Copulation, laying and pleopod egg fixation are particularly affected by salt waters. The only promising application of salinity parameter on astaciculture could be whether prophylactic or curative treatment with concentrated...
Tipo: Text
Palavras-chave: Astacus leptodactylus; Malacostraca; Infectious diseases; Sexual reproduction; Growth; Mortality; Survival; Osmotic adaptations; Crayfish culture; Pathology; Metabolism; Salinity; Ecophysiology; Astaciculture; Régulation osmotique; Reproduction; Survie; Croissance; Pathologie; Ecophysiologie; Salinité; Astacus leptodactylus; Ecrevisse.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1185.pdf

Effects of exposure to petroleum hydrocarbons upon the metabolism of the common sole Solea solea
Provedor de dados: 5
Autores: Davoodi, Fariba; Claireaux, Guy.
On December 12th, 1999, the oil tanker Erika sank off the southern coast of Brittany (France), releasing 20 000–30 000 tons of heavy oil in the open sea. Among the affected coastal habitats were important nurseries for numerous flatfish species and particularly for the common sole, Solea solea. To investigate the potential impact of the spill on this economically significant resource, we employed Fry's concept of metabolic scope for activity to assess the deleterious effect of fuel exposure on the functional integrity of juvenile sole. Fish were captured from uncontaminated areas and experimentally exposed to contamination conditions mimicking those encountered during the weeks that followed the Erika spill. Using respirometry techniques we measured basal...
Tipo: Text
Palavras-chave: Solea solea; Environmental adaptation; Metabolism; Fuel exposure; Erika oil spill.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2798.pdf

Metabolic response to hypoxia in European sea bass (Dicentrarchus labrax) displays developmental plasticity
Provedor de dados: 5
Autores: Cadiz, Laura; Zambonino-infante, Jose-luis; Quazuguel, Patrick; Madec, Lauriane; Le Delliou, Herve; Mazurais, David.
Several physiological functions in fish are shaped by environmental stimuli received during early life. In particular, early-life hypoxia has been reported to have long-lasting effects on fish metabolism, with potential consequences for fish life history traits. In the present study, we examine whether the synergistic stressors hypoxia (40% and 100% air saturation) and temperature (15° and 20 °C), encountered during early life, could condition later metabolic response in European sea bass (Dicentrarchus labrax) juveniles. Growth rate and metabolic parameters related to carbohydrate and lipid metabolism in the liver were investigated at the juvenile stage under normoxic and chronic hypoxic conditions. Juvenile growth rates were significantly lower...
Tipo: Text
Palavras-chave: Hypoxia; Developmental plasticity; Metabolism; European sea bass.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00404/51536/52126.pdf

Activity and composition of the benthic fauna in the Whittard Canyon and the adjacent continental slope (NE Atlantic)
Provedor de dados: 5
Autores: Duineveld, Gérard; Lavaleye, Marc; Berghuis, Eilke; De Wilde, Peter.
We compared the sediment and its community on the Celtic continental slope (Goban Spur) with those in a branch of the nearby Whittard Canyon in search for evidence of canyon mediated transport of (labile) organic matter. We studied the megabenthos and macrobenthos biomass and taxonomic composition, measured in situ sediment community oxygen consumption and determined sediment concentrations of particulate organic carbon, phytopigments, and nucleic acids. While the sediment community and activity on the canyon fan was similar to that on the abyssal station near Goban Spur, the sediment within the canyon had relatively enhanced sediment community oxygen consumption rates and higher levels of phytopigments. particulate organic carbon and nucleic acids,...
Tipo: Text
Palavras-chave: Zone abyssale; Canyon; Macrofaune; Métabolisme; Pigments végétaux; Deep-sea; Canyon; Macrofauna; Metabolism; Phytopigments.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00323/43370/42840.pdf

Biomass, metabolism and nutrition of zooplankton in the Gulf of Lions (NW Mediterranean)
Provedor de dados: 5
Autores: Gaudy, R; Youssara, F; Diaz, F; Raimbault, P.
Zooplankton biomass was measured at 20 stations located along five coastal-offshore transects in the Gulf of Lions (NW Mediterranean) during spring 1998 and winter 1999, using two nets equipped with 200 and 80 Pm mesh. Biomass was twice as abundant in spring. It increased toward the coast and the west of the gulf and was positively correlated to chlorophyll, primary production and organic particulate matter and negatively to temperature and salinity. The enrichment effect of Rhone river input and the oligotrophic influence of the north Mediterranean current (NMC) were the main distribution factors of zooplankton biomass. Higher biomass was obtained in coastal stations with the 80 mum net, which sampled more efficiently young larval stages and nauplii....
Tipo: Text
Palavras-chave: Zooplankton; Métabolisme; Cycle saisonnal; Golfe du Lion; Zooplankton; Metabolism; Seasonal cycle; Gulf of Lions.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00321/43262/42993.pdf

Transferts d'acides amines dans un systeme eau-sediment: Etude preliminaire en milieu experimental
Provedor de dados: 5
Autores: Hermin, M; Daumas, R; Drogue, N.
The bacterial activity of the superficial sediment layer was evaluated by incorporation super(14)C-labelled amino-acids mixed an hydrolysate of chlorella cells. Estimation of super(14)C-CO sub(2) super(14)C and super(14)C-A.A. by H.P.L.C. give some informations on the utilization of free dissolved. A.A. and on the transport of labelled compounds from water to sediment. The adsorption mechanism of A.A. on the mineral particles draw to the sediment a larger part of A.A. in the first period; but 28% of this adsorbed A.A. were discharged by an acid treatment. A part of super(14)C-A.A. was incorporated in unhydrolysable organic matter. Di-COOH A.A. are more resistant to bacterial degradation than the other A.A., but arginine is completely metabolized in ten...
Tipo: Text
Palavras-chave: Biogeochemistry; Bacteria; Chromatographic techniques; Metabolism; Amino acids; Sediment water exchanges.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-945.pdf

Short term variations in feeding and metabolism of Acartia tonsa (pelagic copepod) in the Berre lagoon (France)
Provedor de dados: 5
Autores: Gaudy, R; Pagano, M; Cervetto, G; Saintjean, L; Verriopoulos, G; Beker, B.
Feeding, respiration, and ammonia excretion of Acartia tonsa were studied in a brackish lagoon, near Marseilles (Southern France). The experiments were performed during the same season in two successive years, using naturally occurring particles as food. Strong variations in both quantity and quality of food particles were recorded between the two series of experiments. The ingestion of particles according to their size was studied by means of the Coulter Counter technique. Females ingested more food than males. In both sexes, there was a tendency to select median to large particles, regardless of the shape of the particles-size spectrum. In 1990, the specific production rate, deduced from the energy budget, was equivalent to or greater than the observed...
Tipo: Text
Palavras-chave: Acartia tonsa; Feeding; Metabolism; Production.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00096/20731/18368.pdf

Hepatic phosphoenolpyruvate carboxykinase gene expression is not repressed by dietary carbohydrates in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Panserat, Stéphane; Plagnes Juan, E; Breque, Jeanine; Kaushik, Sadasivam.
Phosphoenolpyruvate carboxykinase (PEPCK) is a rate-limiting enzyme in hepatic gluconeogenesis and therefore plays a central role in glucose homeostasis. The aim of this study was to analyse the nutritional regulation of PEPCK gene expression in rainbow trout (Oncorhynchus mykiss), which are known to use dietary carbohydrates poorly. A full-length hepatic PEPCK cDNA (2637 base pairs with one open reading frame putatively encoding a 635-residue protein) was cloned and found to be highly homologous to mammalian PEPCKs. The presence of a putative peptide signal specific to a mitochondrial-type PEPCK in the deduced amino acid sequence suggests that this PEPCK gene codes for a mitochondrial form, In gluconeogenic tissues such as liver, kidney and intestine,...
Tipo: Text
Palavras-chave: Gluconeogenesis; Nutrition; Metabolism; Glucose; Oncorhynchus mykiss; Rainbow trout; Phosphoenolpyruvate carboxykinase.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-414.pdf

Cultivating the uncultured: limits, advances and future challenges
Provedor de dados: 5
Autores: Alain, Karine; Querellou, Joel.
Since the invention of the Petri dish, there have been continuous efforts to improve efficiency in microbial cultivation. These efforts were devoted to the attainment for diverse growth conditions, simulation of in situ conditions and achievement of high-throughput rates. As a result, prokaryotes catalysing novel redox reactions as well as representatives of abundant, but not-yet cultured taxa, were isolated. Significant insights into microbial physiology have been made by studying the small number of prokaryotes already cultured. However, despite these numerous breakthroughs, microbial cultivation is still a low-throughput process. The main hindrance to cultivation is likely due to the prevailing lack of knowledge on targeted species. In this review, we...
Tipo: Text
Palavras-chave: High throughput cultivation; Cell cell communication; Microbial community; Metabolism; Isolation; Culture.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6820.pdf

Energy-protein management by some warmwater finfishes.
Provedor de dados: 5
Autores: Luguet, P; Moreau, Y.
If warmwater finfish protein requirements as level in diet is low, absolute intake per day is similar to those of coldwater species. But relation of this requirement with specific growth rate differs for strictly warmwater fishes as Tilapias. Analysis of partition between protein and non-protein energy retention shows that these species with Clarias make a better use of non-protein energy provided and consequently improve their protein retention. Better carbohydrate digestibility and metabolism are propounded as part of the explanation.
Tipo: Text
Palavras-chave: Clarias; Oreochromis mossambicus; Oreochromis aureus; Oreochromis niloticus; Tilapia; Cichlidae; Pisces; Nutritional requirements; Digestibility; Diets; Proteins; Metabolism; Carbohydrates; Nutrition; Feeding; Growth rate.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/1989/acte-1424.pdf

Influence of water temperature and oxygenation on the aerobic metabolic scope of Atlantic cod (Gadus morhua)
Provedor de dados: 5
Autores: Claireaux, Guy; Webber, D; Lagardere, Jean-paul; Kerr, S.
Environmental influences (temperature and oxygenation) on cod metabolism and their impact on the ecology of this species were investigated. Limiting oxygen concentration curves (O-2 level ranging between 15 and 100% air saturation) were established at 2, 5 and 10 degreesC. The standard metabolic rate (SMR), the maximum metabolic rate and the metabolic score were then modelled as functions of temperature and/or oxygen saturation. The mean SMR at 2, 5 and 10 degreesC were 19.8 +/- 4.9, 30.8 +/- 6.1 and 54.3 +/- 4.1 mg O-2 h(-1) kg(-1). respectively. Between 2 and 5 degreesC, the active metabolic rate of cod almost doubled from 65 to 120 mg O-2 h(-1) kg(-1), to reach 177 mg O-2 h(-1) kg(-1) at 10 degreesC. In terms of metabolic scope (MS), the temperature...
Tipo: Text
Palavras-chave: Oxygen; Temperature; Physiological ecology; Metabolism; Atlantic cod.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-428.pdf

Maintenance of discrete bacterial populations in adjacent marine habitats.
Provedor de dados: 5
Autores: Robb, F; Muir, D; Davis, C.
Some mechanisms of selection which operate on marine Vibrio and Pseudomonas strains during survival in dilute environments have been examined. Mannitol uptake and utilisation systems are more efficient in the Vibrio strain compared with the Pseudomonas strain during starvation survival. A Vibrio mutant strain lacking mannitol uptake showed no greater loss of viability during starvation survival than mannitol positive strains. The Vibrio strain appears to be constitutive with respect to mannitol uptake systems, whereas the Pseudomonas strain examined is inducible. Active uptake systems for glucose, glutamate, alanine and aspartic acid were efficiently maintained during starvation survival in the Vibrio strain. Results suggest that membrane uptake systems...
Tipo: Text
Palavras-chave: Pseudomonas; Vibrio; Bacteria; Physiology; Alcohols; Food conversion; Survival; Starvation; Metabolism.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-960.pdf

Effects of O-2 supersaturation on metabolism and growth in juvenile turbot (Scophthalmus maximus L.)
Provedor de dados: 5
Autores: Person, Jeannine; Pichavant, Karine; Vacher, Coralie; Le Bayon, Nicolas; Severe, Armelle; Boeuf, Gilles.
Effects of O-2 supersaturation on metabolism and growth were studied in juvenile turbot (Scophthalmus maximus L.). When fish were reared for 30 days in water containing O-2 at 147% or 223% air saturation, there were no significant differences in food intake, growth, food conversion or protein utilization compared to fish exposed to normoxia (100% air saturation in water outlet). Exposure to hyperoxia resulted in increased body fat deposition. Daily rates of O-2 consumption of resting fish were not affected by O-2-concentrations, and there were no significant differences in rates of nitrogenous excretion among fish exposed to the different O-2-concentrations. Turbot tolerated severe hyperoxia, 350% air saturation, for 10 days.There were changes in acid-base...
Tipo: Text
Palavras-chave: Ion regulation; Acid base; Metabolism; Growth; Hyperoxia; Turbot.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-627.pdf

Metabolism of cyanobacteria in anaerobic marine sediments.
Provedor de dados: 5
Autores: Stal, L; Krumbein, W.
Two cyanobacteria isolated from marine microbial mats metabolized endogenous carbon reserves anaerobically in the dark. Microcoleus chthonoplastes reduced elemental sulfur to sulfide and Oscillatoria sp. additionally produced lactate in the absence of elemental sulfur. Under nitrogen fixing conditions, however, no sulfur reduction occurs. Lactate fermentation appeared to be the mechanism of anaerobic carbon degradation under these conditions. With a nitrogenase-reducible substrate, e.g. acetylene, added, lactate fermentation as well as sulfur reduction stops in cultures of Oscillatoria containing nitrogenase. In this case only ethylene production was observed. These cyanobacteria seem to possess the capability to carry out anaerobic dark metabolism...
Tipo: Text
Palavras-chave: Oscillatoria; Microcoleus chthonoplastes; Cyanophyta; Metabolism; Chemical cycles; Algae; Anoxic sediments; Anaerobic respiration; Algal mats; Biogeochemistry; Fermentation; Sulfur; Nitrogen fixation; Nitrogen cycle.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-974.pdf

Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas
Provedor de dados: 5
Autores: Da Costa Gonzalez, Fiz; Robert, Rene; Quere, Claudie; Wikfors, Gary H.; Soudant, Philippe.
Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5...
Tipo: Text
Palavras-chave: Fatty acid; Larvae; Lipids; Metabolism; Oyster; Synthesis.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00268/37962/36086.pdf

Reduced lipid intake leads to changes in digestive enzymes in the intestine but has minor effect on key enzymes of hepatic intermediary metabolism in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Ducasse Cabanot, S; Zambonino, Jose-luis; Richard, N; Medale, Francoise; Corraze, Genevieve; Mambrini, M; Robin, Jean; Cahu, Chantal; Kaushik, Sadasivam; Panserat, S.
For sustainable aquaculture, the removal of marine resource ingredients in fish diets is an important objective. While most studies focus on the replacement of fish oil by vegetable oil, little is known on the nutritional effects of presence (which corresponds to the control diet) or absence of dietary fish oil. We studied fatty acid composition of brush-border membranes and digestive enzyme activities of the intestine and measured the expression and activities of several enzymes involved in the hepatic intermediary metabolism of rainbow trout (Oncorhynchus mykiss) fed for 7 weeks with or without fish oil. The diets were pair-fed to ensure that fish fed either diet had comparable carbohydrate and protein intakes. Absence of fish oil significantly reduced...
Tipo: Text
Palavras-chave: Rainbow trout; Metabolism; Fish oil; Digestion; Aquaculture.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3506.pdf

Modulation des performances cardio-respiratoires du bar europeen (Dicentrarchus labrax) par l'apport d'acides gras alimentaires: une etude integree des effets cardiaques in vivo au courant calcique de type L des myocytes ventriculaires
Provedor de dados: 5
Autores: Chatelier, Aurelien.
Diet quality, and particularly fatty acids composition, is an environmental factor that is emerging as a significant source of physiological diversity. Indeed, numerous recent studies show that the fatty acid composition of the diet influences cardiac and metabolic performances of organisms. Because it is involved in the control and regulation of internal flow of matter and energy, the heart is believed to play a central role in environmental adaptation in fish. Within the double context of adaptation of individuals to their ecological niche and species evolution, understanding sources of interindividual variability and particularly fatty acid influences on the cardiorespiratory system is essential.  The objective of the study was to investigate in the sea...
Tipo: Text
Palavras-chave: Dicentrarchus labrax; Arythmie cardiaque; Hypoxie; Acide oléique; Canal calcique de type L; Calcium; Nage; Métabolisme; Coeur; Acides gras; Dicentrarchus labrax; Cardiac arrhythmias; Hypoxia; Oleic acid; L type calcium channel; Calcium; Metabolism; Heart; Fatty acid.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-2197.pdf

In modelling effects of global warming, invalid assumptions lead to unrealistic projections
Provedor de dados: 5
Autores: Lefevre, Sjannie; Mckenzie, David J.; Nilsson, Goeran E.
Tipo: Text
Palavras-chave: Climate change; Fish; Growth; Metabolism; Modelling; Oxygen uptake; Scaling; Warming.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00426/53762/54697.pdf

Transcriptomic analysis reveals specific metabolic pathways of enterohemorrhagic Escherichia coli O157:H7 in bovine digestive contents
Provedor de dados: 5
Autores: Segura, Audrey; Bertoni, Marine; Auffret, Pauline; Klopp, Christophe; Bouchez, Olivier; Genthon, Clemence; Durand, Alexandra; Bertin, Yolande; Forano, Evelyne.
Background The cattle gastrointestinal tract (GIT) is the main enterohemorrhagic Escherichia coli (EHEC) reservoir. In order to identify nutrients required for the survival or multiplication of EHEC in the bovine GIT, we compared the transcriptomes of the EHEC O157:H7 reference strain EDL933 cultured in vitro in bovine digestive contents (DCs) (rumen, small intestine and rectum) using RNA-sequencing. Results Gene expression profiles showed that EHEC EDL933 activated common but also specific metabolic pathways to survive in the different bovine DCs. Mucus-derived carbohydrates seem important in EHEC nutrition in posterior DCs (small intestine and rectum) but not in rumen content. Additional carbohydrates (xylose, ribose, mannitol, galactitol) as well as...
Tipo: Text
Palavras-chave: EHEC O157:H7; Bovine gastrointestinal tract; RNA-seq; Metabolism; Carbon sources; Mucus-derived carbohydrates; Ethanolamine; Respiration.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00464/57563/59734.pdf

Biologie de la cellule normale et pathologique : explorer la fonction des protéines
Provedor de dados: 5
Autores: Corporeau, Charlotte.
J’appartiens aux scientifiques qui étudient le monde animal, et non pas le végétal (moins bons souvenirs…). Mon échelle de travail est toute petite, c’est le compartiment intracellulaire, car j’étudie les protéines. Comment comprendre leur rôle ? Comment régulent-elles le fonctionnement normal de la cellule ? Comment renseignent-elles sur l’environnement cellulaire? Quel impact du stress sur leur fonction? Comment différencier les réponses physiologiques des réponses pathologiques ? Des modèles vertébrés et invertébrés, plusieurs types cellulaires, la découverte de fonctions: je vais décrire dans mon HDR les ingrédients qui ont produit la science à laquelle j’ai participée depuis 1995. Je vais exposer quelques scénarios de ces épisodes passés en...
Tipo: Text
Palavras-chave: Protéomiuqe; Invertébrés marins; Métabolisme; Découverte de nouveaux médicament; Proteomics; Marine inverebrate; Metabolism; Drug discovery.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00676/78781/80970.pdf

Effets des caractéristiques physico-chimiques et biorhéologiques de l'eau sur la physiologie, les performances adaptatives et la sélection de l'habitat chez la sole commune Solea solea (Linnaeus, 1758)
Provedor de dados: 5
Autores: Couturier, Christine.
Shellfish culture can lead to an accumulation of exopolysaccharides (EPS) over the surface of intertidal mudflats. EPS are rheologically active polymers that change water viscosity and water flow over the branchial basket of the juvenile flatfish that colonise intertidal mudflats. The aim of this study was to assess the impact of water EPS content on various aspects of the physiology and behaviour of the common sole Solea solea. Field studies revealed that water total EPS can be up to 15 mg l 1 in the field. In sole juveniles (aged class 0+) ventilatory distress was observed at concentrations above 4 mg l-1, and the intensity of the constraint decreased with fish size. Nonetheless, neither standard metabolic rate nor tolerance of hypoxia were influenced...
Tipo: Text
Palavras-chave: Flatfish; SDA; Growth; Habitat selection; Metabolism; Hypoxia; EPS; Viscosity; Common sole; Poissons plats; SDA; Croissance; Sélection d'habitat; Métabolisme; Hypoxie; EPS; Viscosité; Sole commune.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/these-3461.pdf

Metabolism of CO and CH sub(4) by nitrifiers and the determination of the nitrification rate.
Provedor de dados: 5
Autores: Morita, R; Jones, R.
The nitrifying bacteria were found to survive 24 weeks in the absence of ammonium without decreasing their number of cell size. Because H sub(2), CO, and CH sub(4) are present in the marine environment, these substrates were investigated as a possible source of the energy of maintenance for the nitrifying bacteria. super(14)CO and super(14)CH sub(4) were found to be oxidized by the nitrifiers. N-serve was found to inhibit the oxidation of CO. Using the nitrifiers' ability to oxide CO, a method for the determination of the nitrification rate was developed. The ability of nitrifiers to oxidize CO may play a significant role in the cycling of CO sub(2) in the marine environment. Whether CO and CH sub(4) oxidation play a role in the survival of nitrifiers in...
Tipo: Text
Palavras-chave: Methane; Carbon dioxide; Survival; Bacteria; Nitrification; Nitrogen cycle; Oxidation; Carbon cycle; Biogeochemistry; Metabolism.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-975.pdf

Comparative analysis of oxygen consumption rates between cupped oyster spat of Crassostrea gigas of French, Japanese, Spanish and Taiwanese origins
Provedor de dados: 5
Autores: Goulletquer, Philippe; Wolowicz, Maciej; Latala, Adam; Geairon, Philippe; Huvet, Arnaud; Boudry, Pierre.
Respiration rates of various geographical strains of Crassostrea gigas were compared to assess the respiratory expenditure as a physiological indicator of catabolism (cost of maintenance). Parental oysters, sampled in France (Marennes-Oleron), Japan (Hiroshima), Taiwan (Tunkang) and Spain (Cadix), were differentiated by both their geographic origin and by mitochondrial DNA markers, allowing the distinction between the two closely related taxa Crassostrea gigas and Crassostrea angulata. After reproduction of these parental oysters, respiration rates of spat of each strain, reared under common controlled conditions, were individually estimated at 20 degrees C by using a volumetric microrespirometer. Our results demonstrated that physiological variability...
Tipo: Text
Palavras-chave: Pacific Ocean; NE Atlantic; Oyster strains; Hybrids; Crassostrea angulata; Crassostrea gigas; Metabolism; Respiration rates; Océan Pacifique; Atlantique NE; Souches génétiques; Hybrides; Crassostrea angulata; Crassostrea gigas; Métabolisme; Taux de respiration.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-859.pdf

Differential protein expression profile in the liver of pikeperch (Sander lucioperca) larvae fed with increasing levels of phospholipids
Provedor de dados: 5
Autores: Hamza, Neila; Silvestre, Frederic; Mhetli, Mohamed; Ben Khemis, Ines; Dieu, Marc; Raes, Martine; Cahu, Chantal; Kestemont, Patrick.
A comparative proteomic approach was used to assess the protein expression profile in the liver of 34 days old pikeperch larvae fed from day 10 post hatching, with three isoproteic and isolipidic formulated diets varying by their phospholipid (PL) contents (% dry diet weight): 1.4% (PL1), 4.7% (PL5) and 9.5% (PL9). Using 2D-DIGE minimal labelling of liver extracts, we were able to show 56 protein spots with a differential intensity (p&lt;0.05) depending on the dietary PL content. Among these spots, 11 proteins were un-ambiguously identified using nanoLC-MS/MS tandem mass spectrometry. In the PL9 larvae, our results indicate that the glycolytic pathway could be down-regulated due to the under-expression of the fructose biphosphate aldolase B and the...
Tipo: Text
Palavras-chave: 2D-DIGE; Metabolism; Phospholipid; Pikeperch; Proteomics.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00007/11837/8575.pdf

Etude des liens trophiques au sein d'une communaute bacterienne selectionnee a partir d'un sediment marin sur le cyclohexene-4 dicarboximide-1,2 (THPI)
Provedor de dados: 5
Autores: Terrom, G; Gil, G; Lepetit, J.
A bacterial community has been selected from a marine sediment on 4-cyclohexene-1,2 dicarboximide or THPI (fungicide). This community consists of nine strains. The "principal" strain, Photobacterium mandapamensis , degrades THPI by different metabolic pathways many of which require co-oxidative sequences. The other eight "auxiliary" strains occur at different states along the trophic chain formed by the "principal" strain, so increasing velocity and intensity of the THPI biodegradation.
Tipo: Text
Palavras-chave: Bacteria; Marine pollution; Microorganisms; Metabolism; Fungicides; Biodegradation; Sediment pollution; Trophic relationships.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1021.pdf

Etude de la composition corporelle de la sole (Solea solea ) au cours du jeune: Influence de la temperature
Provedor de dados: 5
Autores: Richard, P; Djabali, M; Lagardere, Francoise; Lagardere, J.
The effects of starvation on Solea solea at 10 and 20 degree C in sea water, were studied. At 20 degree C fishes start to die on week 4; at 10 degree C they support a longer starvation period (9 weeks). Proximate analysis shows that protein are more steadily used at 20 degree C than at 10 degree C, contrarily to the corporal lipids which are used at the same rate. Starvation results in a reduction of liver lipids and glycogen. This reduction is more pronounced at 20 degree C : 70% of hepatic glycogen is used at this temperature within the first week of starvation against 30% at 10 degree C.
Tipo: Text
Palavras-chave: Solea solea; Soleidae; Pisces; Survival; Tissues; Biochemical composition; Temperature effects; Proteins; Lipids; Starvation; Metabolism.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1246.pdf

Differential gene expression after total replacement of dietary fish meal and fish oil by plant products in rainbow trout (Oncorhynchus mykiss) liver
Provedor de dados: 5
Autores: Panserat, S.; Hortopan, G. A.; Plagnes-juan, E.; Kolditz, C.; Lansard, M.; Skiba-cassy, S.; Esquerre, D.; Geurden, I.; Medale, Francoise; Kaushik, Sadasivam; Corraze, Genevieve.
Our objective was to analyse the hepatic transcriptomes of juvenile rainbow trout fed with a plant-based diet. We focused our analysis on the total replacement of fish meal (FM) and fish oil (FO) by a 100% plant-based diet (0% FM, 0% FO). We analysed the postprandial hepatic transcriptomes of rainbow trout fed the two diets 8 h after feeding. Six total hepatic RNAs from each dietary group were hybridised against a trout cDNA microarray (9K). After treatment of the data respecting the standard MIAME (Minimum Information About a Microarray Experiment) protocol, we found that 176 hepatic genes were differentially expressed between fish fed the two diets: 96 and 80 were over-expressed and under-expressed, respectively, in trout fed the plant-based diet. A...
Tipo: Text
Palavras-chave: Rainbow trout; Transcriptome; Metabolism; Liver; Vegetable products; Marine ingredients.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6684.pdf

Cobalamin Scarcity Modifies Carbon Allocation and Impairs DMSP Production Through Methionine Metabolism in the Haptophyte Microalgae Tisochrysis lutea
Provedor de dados: 5
Autores: Nef, Charlotte; Henry, Céline; Nicolau, Elodie; Bérard, Jean-baptiste; Hervé, Fabienne; Caruana, Amandine; Kaas, Raymond; Mairet, Francis; Garnier, Matthieu.
Cobalamin (vitamin B12) is a cobalt-containing enzymatic cofactor involved in methionine synthesis. Provided only by select bacteria and archaea in marine systems, this vitamin is known to limit primary production in different oceanic areas. Understanding the consequences of cobalamin limitation on phytoplankton physiology is of great interest, notably for cobalamin-dependent haptophytes that significantly contribute to oceanic carbon fixation and sulfur cycle through dimethyl sulfonio propionate (DMSP) synthesis. Here, the effect of cobalamin limitation was compared to nitrogen limitation on the model haptophyte Tisochrysis lutea grown in chemostats, combining comparative proteomics with the analysis of major macromolecules and specific osmolytes. Our...
Tipo: Text
Palavras-chave: Cobalamin; Vitamin B-12; Haptophyte microalgae; Tisochrysis lutea; DMSP; Chemostat; Comparative proteomics; Metabolism.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00654/76575/77711.pdf

Etude cytologique ultrastructurale des anomalies hepatiques du loup, de la daurade, et de l'anguille, induites par une alimentation artificielle
Provedor de dados: 5
Autores: Bac, N; Biagianti, S; Brusle, J.
In the three species Dicentrarchus labrax, Sparus auratus , and Anguilla anguilla an artificial feeding (dry pellets) disturbs liver cells as revealed at ultrastructural level. In hepatocytes, anomalies consist of lipids storage (liposomes) considered as deviation in fatty acids metabolism. Moreover are observed cellular alterations (mitochondrial vacuolization, nuclear pycnosis) related to degenerative process. It is of great interest to understand wether liver modifications are just a reaction to an unbalanced feeding or a true trophic pathology.
Tipo: Text
Palavras-chave: Dicentrarchus labrax; Serranidae; Pisces; Natural populations; Fish culture; Lipids; Fatty acids; Cytology; Ultrastructure; Cells; Diseases; Pellet feeds; Metabolism; Liver; Artificial feeding; Nutrition disorders.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1250.pdf

Influence of CH4 and H2S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel Bathymodiolus azoricus
Provedor de dados: 5
Autores: Riou, V.; Halary, S.; Duperron, S.; Bouillon, S.; Elskens, M.; Bettencourt, R.; Santos, R. S.; Dehairs, F.; Colaco, A.
High densities of mussels of the genus Bathymodiolus are present at hydrothermal vents of the Mid-Atlantic Ridge. It was previously proposed that the chemistry at vent sites would affect their sulphide- and methane-oxidizing endosymbionts' abundance. In this study, we confirmed the latter assumption using fluorescence in situ hybridization on Bathymodiolus azoricus specimens maintained in a controlled laboratory environment at atmospheric pressure with one, both or none of the chemical substrates. A high level of symbiosis plasticity was observed, methane-oxidizers occupying between 4 and 39% of total bacterial area and both symbionts developing according to the presence or absence of their substrates. Using (HCO3-)-C-13 in the presence of sulphide, or...
Tipo: Text
Palavras-chave: Mid-atlantic ridge; Hydrocarbon-seep mussel; Deep-sea mussels; Hydrothermal-vent; Puteoserpentis bivalvia; Mytilidae; Methane; Endosymbionts; Bacteria; Metabolism.
Ano: 2008
URL: https://archimer.ifremer.fr/doc/00207/31865/30271.pdf

Microbial Diversity and Biosignatures: An Icy Moons Perspective
Provedor de dados: 5
Autores: Jebbar, Mohamed; Hickman-lewis, Keyron; Cavalazzi, Barbara; Taubner, Ruth-sophie; Rittmann, Simon K.-m. R.; Antunes, Andre.
The icy moons of the outer Solar System harbor potentially habitable environments for life, however, compared to the terrestrial biosphere, these environments are characterized by extremes in temperature, pressure, pH, and other physico-chemical conditions. Therefore, the search for life on these icy worlds is anchored on the study of terrestrial extreme environments (termed “analogue sites”), which harbor microorganisms at the frontiers of polyextremophily. These so-called extremophiles have been found in areas previously considered sterile: hot springs, hydrothermal vents, acidic or alkaline lakes, hypersaline environments, deep sea sediments, glaciers, and arid areas, amongst others. Such model systems and communities in extreme terrestrial environments...
Tipo: Text
Palavras-chave: Extremophiles; Prokaryotes; Metabolism; Diversity; Adaptation; Space explorat.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00606/71771/70275.pdf

Effect of an herbivorous diet on energy balance of Litopenaeus vannamei at selected ontogenetic stages
Provedor de dados: 5
Autores: Maldonado, Carlos; Cuzon, Gerard; Guzman, Emilio; Brito, Roberto; Soto, Luis; Arena, Leticia; Gaxiola, Gabriela.
Herbivorous (20% vegetable protein, 40% carbohydrate) and carnivorous diets (40% marine animal protein, 20% carbohydrate) were experimentally tested to assess their effect on the energy balance and energetic substrates utilized by postlarvae (PL's /15 days) and juvenile shrimp (3-6 g) of Litopenaeus vannamei. Postlarval stage 60 (PL60, early juveniles) shrimps fed HeD and CaD diets, then late juveniles (3-6 g) acclimated to the same diets were tested for their respective energy partitioning potential. No significant differences (p&gt;0.05) on growth were obtained in early juveniles (mean final wet weight of 0.19 g). However in late juvenile stages a significant difference (p&lt;0.05) in growth rate was observed. In terms of energy partitioning, both early...
Tipo: Text
Palavras-chave: Energy; Juveniles; Metabolism; Protein sources; Shrimp.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-7402.pdf

Oxygen and carbon dioxide fluxes at the water-sediment interface of a tropical lagoon
Provedor de dados: 5
Autores: Boucher, Guy; Clavier, Jacques; Garrigue, Claire.
Oxygen demand, carbon dioxide release and total alkalinity shift were calculated from changes in oxygen, pH and total alkalinity produced by bottom dark incubations at the water-sediment interface of the 3 bottom types identified in a southwest lagoon in New Caledonia. Total sediment oxygen demand (AO?) was corrected from nonbioloyical oxygen demand (NBOD) In order to obtain the apparent biological activity (AOlO)T.o tal carbon dioxide flux (AC02)w as corrected from total alkalinity shift in order to estimate organic carbon processes. The resulting mean carbon dioxide flux (ACOzO= 2.58 mm01 m-' h-', SE = 0.12) exceeded biological oxygen demand (AO," = 1.60 mm01 m-' h-', SE = 0.08). The highly significant ratio estimates from functional regression lines of...
Tipo: Text
Palavras-chave: Carbon flux; Metabolism; Tropical lagoon.
Ano: 1994
URL: https://archimer.ifremer.fr/doc/00296/40745/39738.pdf

Sprint swimming performance of juvenile European sea bass
Provedor de dados: 5
Autores: Nelson, J; Claireaux, Guy.
Despite its potential for determining animal success in a variety of ecologically important situations, very little is known about the sprint swimming performance of fishes. To evaluate whether selection on sprint swimming performance occurs in the wild, one must first demonstrate measurable and reproducible variation of sprint performance. In this study, we employ a computer-controlled laser detection system to study the sprint performance of 75 juvenile European sea bass Dicentrarchus labrax raised under uniform conditions in the laboratory. This percichthyid is a prized sport and food fish in Europe and similar ecologically to the North American striped bass Morone saxatilis. Individual European sea bass were subjected to endurance swimming performance...
Tipo: Text
Palavras-chave: Dicentrarchux labrax; Juveniles; Computer laser detection; Endurance; Metabolism; Swimming performance; Fish.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-887.pdf

Effects of environmental conditions on the sessile existence of an estuarine sediment bacterium.
Provedor de dados: 5
Autores: Geesey, G; Salas, S; Mittelman, M.
A submerged glass coverslip technique was developed to determine dissolved organic nutrient uptake by adherent cells of a sediment isolate of Enterobacter cloacae . Cells which colonized the coverslips in swirling culture flasks during anaerobic growth remained firmly attached during manipulations employed to determine uptake of radiolabeled glucose by the adherent population. The attached cells were capable of a more rapid rate of glucose uptake than free cells. Lineweaver-Burk plots demonstrated different glucose uptake kinetics for the 2 cell populations. The data suggest that physiological changes occur in cells soon after they become attached to surfaces. These changes appear to enhance the metabolic activity of the adherent population.
Tipo: Text
Palavras-chave: Enterobacter cloacae; Adhesion; Glucose; Environmental effects; Bacteria; Sediments; Estuaries; Sessile species; Culture media; Metabolism; Dissolved oxygen; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-967.pdf

Models projecting the fate of fish populations under climate change need to be based on valid physiological mechanisms
Provedor de dados: 5
Autores: Lefevre, Sjannie; Mckenzie, David J.; Nilsson, Goran E.
Some recent modelling papers projecting smaller fish sizes and catches in a warmer future are based on erroneous assumptions regarding (i) the scaling of gills with body mass and (ii) the energetic cost of 'maintenance'. Assumption (i) posits that insurmountable geometric constraints prevent respiratory surface areas from growing as fast as body volume. It is argued that these constraints explain allometric scaling of energy metabolism, whereby larger fishes have relatively lower mass-specific metabolic rates. Assumption (ii) concludes that when fishes reach a certain size, basal oxygen demands will not be met, because of assumption (i). We here demonstrate unequivocally, by applying accepted physiological principles with reference to the existing...
Tipo: Text
Palavras-chave: Aerobic scope; Gill surface area; Growth; Metabolism; Oxygen consumption; Respiration; Scaling.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00395/50621/51449.pdf

Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Goulletquer, Philippe; Wolowicz, Maciej; Latala, Adam; Brown, Craig; Cragg, Simon.
The application of a simple, fast and reliable volumetric microrespirometric method to assess respiration of bivalve larvae is discussed. As a model, C. gigas larvae of various sizes were used. Metabolic activity of veliger larvae was assessed by measuring respiratory rate for use in ecophysiological modelling. As an example of the application of this approach, additional measurements of veliger respiratory rates were carried out to assess the effect on larval metabolism of different concentrations of leachate from wood treated with chromated copper-arsenate (CCA). Veligers of Crassostrea gigas (length from 95 to 331 pm) were fed with a mixture of Isochrysis galbana) and Chaetoceros pumilum cells. Experiments were performed in a 20 degreesC constant...
Tipo: Text
Palavras-chave: Crassostrea gigas; Leachate; CCA; Volumetric micro respirometry; Larvae; Metabolism; Respiratory rate.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-399.pdf

Allocation du carbone et métabolisme azoté chez l'haptophyte Tisochrysis lutea
Provedor de dados: 5
Autores: Garnier, Matthieu.
The aim of this thesis is to improve knowledge on mechanisms involved in the response to nitrogen limitation and in lipid accumulation in the microalgae haptophyte Tisochrysis lutea. The wild type strain and a lipid accumulating mutant strain were grown under different nitrogen limitation and starvation and analyzed by functional genomics. Four genes of high-affinity nitrate/nitrite transporter (Nrt2) were identified and characterized to reveal the mechanisms involved in mineral absorption in this species. Transcriptomes of both strains were sequenced and proteins affected by nitrogen starvation and differentially expressed between the two strains were identified. We so identified the functions regulated by nitrogen deficiency and potentially involved in...
Tipo: Text
Palavras-chave: Microalgues; Métabolisme; Azote; Carbone; Lipides; Genomique fonctionelle; Protéomique; Transcriptomique; Microalgae; Metabolism; Nitrogen; Carbon; Lipids; Functional genomics; Proteomics; Transcriptomics.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00353/46378/46000.pdf

Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa
Provedor de dados: 5
Autores: Demoy-schneider, Marina; Schmitt, Nelly; Le Pennec, Gael; Suquet, Marc; Cosson, Jacky.
High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J...
Tipo: Text
Palavras-chave: Spermatozoa quality; Cryopreservation; Pinctada margaritifera; Motility; Metabolism; CASA.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00453/56421/58114.pdf

Low pH reduced survival of the oyster Crassostrea gigas exposed to the Ostreid herpesvirus 1 by altering the metabolic response of the host
Provedor de dados: 5
Autores: Fuhrmann, Marine; Richard, Gaëlle; Quere, Claudie; Petton, Bruno; Pernet, Fabrice.
Environmental change in the marine realm has been accompanied by emerging diseases as new pathogens evolve to take advantage of hosts weakened by environmental stress. Here we investigated how an exposure to reduced seawater pH influenced the response of the oyster Crassostrea gigas to an infection by the Ostreid herpesvirus type I (OsHV-1). Oysters were acclimated at pH 8.1 or pH 7.8 and then exposed to OsHV-1. Their survival was monitored and oyster tissues were sampled for biochemical analyses. The survival of oysters exposed to OsHV-1 at pH 7.8 was lower (33.5%) than that of their counterparts at pH 8.1 (44.8%) whereas levels of OsHV-1 DNA were similar. Energetic reserves, fatty acid composition and prostaglandin levels in oyster did not vary...
Tipo: Text
Palavras-chave: Acidification; Bivalve; Disease; Environment; Metabolism; Mortality risk.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00472/58334/60887.pdf

Chitin/toxicant interactions and microbial degradation within estuarine ecosystems.
Provedor de dados: 5
Autores: Portier, R; Meyers, S.
The environmental effect and fate of several agricultural and industrial source toxicants were evaluated in controlled laboratory microcosm systems in the presence of the aminopolysaccharide polymer, chitin. Toxicants examined included methyl parathion, phenol, pentachlorophenol, 2,4 dichlorophenol, 1,2 dichloroethane and Kepone. Microcosm operating parameters, including pH/Eh, salinity, temperature, flow rate and sediment/water interface conditions, were based on comparable in situ measurements. Fate analysis predictions were based on mineralization of the parent toxicant compound as measured by chromatographic analysis or super(14)CO sub(2) expiration. Chitin, amended to continuous flow microcosms, promoted either cometabolic or cooxidative...
Tipo: Text
Palavras-chave: Controlled conditions; Chemical pollutants; Industrial wastes; Pesticides; Metabolism; Food webs; Biodegradation; Ecosystems; Microorganisms; Environmental effects; Brackishwater pollution; Estuaries; Toxicants; Chitin.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1011.pdf

Reconstructing individual food and growth histories from biogenic carbonates
Provedor de dados: 5
Autores: Pecquerie, Laure; Fablet, Ronan; De Pontual, Helene; Bonhommeau, Sylvain; Alunno-bruscia, Marianne; Petitgas, Pierre; Kooijman, Sebastiaan A. L. M.
Environmental conditions experienced by aquatic organisms are archived in biogenic carbonates such as fish otoliths, bivalve shells and coral skeletons. These calcified structures present an accretionary growth and variations in optical properties - color or opacity - that are used to reconstruct time. Full and reliable exploitation of the information extracted from these structures is, however, often limited as the metabolic processes that control their growth and their optical properties are poorly understood. Here, we propose a new modeling framework that couples both the growth of a biogenic carbonate and its optical properties with the metabolism of the organism. The model relies on well-tested properties of Dynamic Energy Budget (DEB) theory. It is...
Tipo: Text
Palavras-chave: Otolith; Calcification; Metabolism; Bioenergetic model; Food reconstruction; Dynamic Energy Budget theory.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00060/17082/14593.pdf

Expression of candidate genes related to metabolism, immunity and cellular stress during massive mortality in the American oyster Crassostrea virginica larvae in relation to biochemical and physiological parameters
Provedor de dados: 5
Autores: Genard, Bertrand; Moraga, Dario; Pernet, Fabrice; David, Elise; Boudry, Pierre; Tremblay, Rejean.
Quantification of mRNA of genes related to metabolism, immunity and cellular stress was examined in relation to a massive mortality event during the culture of American oyster larvae, Crassostrea virginica which was probably, in regard to previous microbiological analysis, induced by vibrio infection. To document molecular changes associated with the mortality event, mRNA levels were compared to biochemical and physiological data, previously described in a companion paper. Among the 18 genes studied, comparatively to the antibiotic control, 10 showed a lower relative gene expression when the massive mortality occurred. Six of them are presumed to be related to metabolism, corroborating the metabolic depression associated with the mortality event suggested...
Tipo: Text
Palavras-chave: Massive mortality; Relative gene expression; Metabolism; Immunity; Cellular stress; Larvae.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00075/18585/16138.pdf

Effects of Heating on Proportions of Azaspiracids 1–10 in Mussels (     Mytilus edulis     ) and Identification of Carboxylated Precursors for Azaspiracids 5, 10, 13, and 15
Provedor de dados: 5
Autores: Kilcoyne, Jane; Mccarron, Pearse; Hess, Philipp; Miles, Christopher O.
Azaspiracids (AZAs) are marine biotoxins that induce human illness following the consumption of contaminated shellfish. European Union regulation stipulates that only raw shellfish are tested, yet shellfish are often cooked prior to consumption. Analysis of raw and heat-treated mussels (Mytilus edulis) naturally contaminated with AZAs revealed significant differences (up to 4.6-fold) in AZA1–3 (1–3) and 6 (6) values due to heat-induced chemical conversions. Consistent with previous studies, high levels of 3 and 6 were detected in some samples that were otherwise below the limit of quantitation before heating. Relative to 1, in heat-treated mussels the average (n = 40) levels of 3 (range, 11–502%) and 6 (range, 3–170%) were 62 and 31%, respectively. AZA4...
Tipo: Text
Palavras-chave: Azaspiracid; Decarboxylation; Hydroxylation; Chemical conversion; Heating mass spectrometry; Metabolism.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00303/41433/41005.pdf

Métabolisme et interactions bactériennes en lien avec la vitamine B12 chez la microalgue haptophyte Tisochrysis lutea
Provedor de dados: 5
Autores: Nef, Charlotte.
Vitamin B12 is an enzymatic cofactor that enables methionine synthesis and whose availability can control phytoplankton development in marine systems. This vitamin is synthesized only by select species of bacteria and archaea. Haptophyte microalgae form a widespread group with major ecological importance. However, little is known about their utilization of vitamin B12 nor their interactions with B12 producers. Our main objectives were to characterize mechanisms of acquisition and utilization of vitamin B12 by Haptophytes and the effect of B12 limitation on their metabolism by considering the model species Tisochrysis lutea. Analysis of the repartition of B12 dependency in haptophytes by molecular approaches suggest that this group would be the first to...
Tipo: Text
Palavras-chave: Métabolisme; Vitamine B12; Interactions microbiennes; Microalgues haptophytes; Metabolism; Vitamin B12; Microbial interactions; Haptophyte microalgae.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00598/71003/69268.pdf

Variations of zooplankton metabolism and feeding in the frontal area of the Alboran Sea (western Mediterranean) in winter
Provedor de dados: 5
Autores: Gaudy, R; Youssara, F.
Respiration, excretion (NH4 and PO4) and feeding of zooplankton from the upper 200 m of the Almeria-Oran frontal region (western Mediterranean), were studied during winter 1997. Eight sites belonging to the three main hydrodynamic structures of the area (Mediterranean water, geostrophic Atlantic jet and associated anticyclonic gyre) were considered. Lowest values of respiration, PO4 excretion, O/P atomic ratio and particle ingestion were found in Mediterranean waters. At the frontal limit, respiration and ammonium excretion were enhanced compared to the adjacent structures (Mediterranean water and Atlantic jet). In the gyre water, lowest O/N and highest N/P values were observed and maximum faecal pellet production and reproduction activity (inferred from...
Tipo: Text
Palavras-chave: Mer d'Alboran; Front; Zooplancton; Métabolisme; Nutrition; Alboran sea; Front; Zooplankton; Metabolism; Feeding.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00322/43275/43006.pdf

Effects of water viscosity upon ventilation and metabolism of a flatfish, the common sole Solea solea (L.)
Provedor de dados: 5
Autores: Couturier, Christine; Rouault, Alice; Mckenzie, David; Galois, Robert; Robert, Serge; Joassard, Lucette; Claireaux, Guy.
The French Atlantic coast contains large highly productive intertidal mudflats that are colonised by juveniles of numerous flatfish species, including the common sole (Solea solea, L.). These ecosystems are also heavily exploited by the shellfish farming industry. Intensive bivalve culture is associated with substantial biodeposition (1-6 t-dw ha(-1) day(-1)), which directly or indirectly contributes to increase exopolysaccharide (EPS) concentrations at the interface between water column and seabed. EPS are long-chain molecules organised into colloids, which influence rheological properties of water, particularly viscosity. Increased water viscosity had consequences for ventilatory activity of juvenile flatfish, whereby the minimal pressure required to...
Tipo: Text
Palavras-chave: EPS; Hypoxia; Metabolism; Water viscosity; Biodeposition; Solea solea.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2801.pdf

Role of bacteria in the gastral cavity of Anthozoa.
Provedor de dados: 5
Autores: Herndl, G; Velimirov, B.
Bacterial density of the coelenteric fluid (CF) of some anthozoan species was determined in situ and under laboratory conditions. In all species examined bacterial density of the CF was higher in the gastral cavity than in ambient ranging from 10 super(5) up to 5 x 10 super(6) cells. ml super(-1). Incubation experiments with the CF indicate that a bacterial population within the coelenteron is efficiently controlled by the CF, favoring bacterial growth if bacterial densities are low in the CF and showing clearance activity at high bacterial densities. Feeding of pre-starved anthozoans caused a rapid response of coelenteric rod-shaped bacteria. Evidence is presented that coelenteric bacteria are digested periodically although they contribute 1% to the...
Tipo: Text
Palavras-chave: Anthozoa; Coelomic fluids; Bacteria; Microorganisms; Interspecific relationships; Metabolism; Nutrition.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-989.pdf

Effects of repeated hypoxic shocks on growth and metabolism of turbot juveniles
Provedor de dados: 5
Autores: Person, Jeannine; Lacut, Anne-sophie; Le Bayon, Nicolas; Le Roux, Annick; Pichavant, Karine; Quemener, Loic.
Turbot juveniles (45 g) were exposed for 41 d (17 C, 34parts per thousand salinity) to constant normoxic (100-100% air saturation, 100-100) or moderate hypoxic (75-75% air saturation, 75-75) conditions and to repeated hypoxic shocks (20% saturation for 1 h, 5 d per week) from normoxic (100-20% air saturation. 100-20) or moderate hypoxic (75-20% air saturation, 75-20) conditions. A normoxic group was feed restricted (100-FR). Mass increase of 100-100 and 75-75 groups fed to satiation was not significantly different. In comparison, it was significantly lower in the 100-20 and 75-20 groups (NS between the two hypoxic shocks groups). Intermediate results were obtained in the 100-100-FR group. The lowest mass increase under hypoxic shocks was explained by a...
Tipo: Text
Palavras-chave: Turbot; Metabolism; Growth; Hypoxic shock.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-571.pdf

L'absorption intestinale des lipides chez la truite arc-en-ciel (Salmo gairdneri )
Provedor de dados: 5
Autores: Vernier, J; Sire, M.
Though the absorption of dietary lipids is much slower in S. gairdneri than in mammals, the mechanism does not differ fundamentally. The long chain fatty acids resulting from intraluminal hydrolysis are esterified by the epithelial cells and transferred as VLDL-like particles and chylomicrons to lymph. The size of granules depends on the nature of the ingested fatty acids. The intracellular stages of this lipoprotein synthesis are defined.
Tipo: Text
Palavras-chave: Salmonidae; Pisces; Lymph; Fatty acids; Nutrition; Lipoproteins; Lipids; Intestines; Food absorption; Metabolism.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1245.pdf

Metabolism of the Pacific oyster,     Crassostrea gigas     , is influenced by salinity and modulates survival to the Ostreid herpesvirus OsHV-1
Provedor de dados: 5
Autores: Fuhrmann, Marine; Delisle, Lizenn; Petton, Bruno; Corporeau, Charlotte; Pernet, Fabrice.
The Pacific oyster, Crassostrea gigas, is an osmoconforming bivalve exposed to wide salinity fluctuations. The physiological mechanisms used by oysters to cope with salinity stress are energy demanding and may impair other processes, such as defense against pathogens. This oyster species has been experiencing recurrent mortality events caused by the Ostreid herpesvirus 1 (OsHV-1). The objectives of this study were to investigate the effect of salinity (10, 15, 25 and 35‰) on energetic reserves, key enzyme activities and membrane fatty acids, and to identify the metabolic risk factors related to OsHV-1-induced mortality of oysters. Acclimation to low salinity led to increased water content, protein level, and energetic reserves (carbohydrates and...
Tipo: Text
Palavras-chave: Bivalve; Disease; Environment; Metabolism; Mortality risk; Salinity.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00427/53886/54958.pdf

Biomass distribution and physiological capabilities of bacteria in the water column above a sea grass system.
Provedor de dados: 5
Autores: Velimirov, B; Herndl, G; Kavka, G.
The distribution pattern of bacteria in the water column above and within a Posidonia oceanica bed near Ischia (off the Gulf of Naples) was investigated and correlated with DOC and POM data for autumn and winter. Data from samples of 5 stations along a transect from shallow to deep showed rather low bacterial densities (10 super(4).ml super(-1)) for both seasons. The number of attached bacteria was higher in late winter as compared to autumn when the leaves are shed from the stands. Except for occasional peaks DOC ranged between 1.5 and 4.5 mg C/1 while POM concentrations ranged form 4 to 14 mg AFDW.1 super(-1). Hydrolytic capabilities of isolated bacterial colonies showed that more carbohydrate substrates could be fermented in winter as compared to...
Tipo: Text
Palavras-chave: Posidonia oceanica; Microorganisms; Physiology; Seasonal distribution; Algae; Bacteria; Particulate organic matter; Metabolism; Water column; Fermentation; Sea grass.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-954.pdf

Allelochemicals from Alexandrium minutum induce rapid inhibition of metabolism and modify the membranes from Chaetoceros muelleri
Provedor de dados: 5
Autores: Long, Marc; Tallec, Kevin; Soudant, Philippe; Le Grand, Fabienne; Donval, Anne; Lambert, Christophe; Sarthou, Geraldine; Jolley, Dianne F.; Hegaret, Helene.
Allelochemical interactions are likely to be a contributing factor explaining the success of large blooms of the harmful marine dinoflagellate Alexandrium, however, the physiological mechanisms of allelochemical interactions remain poorly described. Here we investigated the sub-lethal effects (on an hourly scale) of a filtrate containing allelochemicals from Alexandrium minutum on the physiology of the common diatom Chaetoceros muelleri. The filtrate induced deleterious effects to the diatom physiology within only 30 min of exposure. Esterase activity and photosynthesis were drastically inhibited, with up to 34% of the population being metabolically inactive and up to 30% reduction in photosystem II quantum yield when exposed to the filtrate. In addition,...
Tipo: Text
Palavras-chave: Allelopathy; Alexandrium; Metabolism; Photoinhibition; Lipids; Pigments.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00460/57133/59748.pdf

Mise au point d'un outil de caractérisation de l'état physiologique des cheptels d'huîtres. Etude expérimentale sur des familles génétiques de l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: De Ambroggi, Céline.
This study is in keeping with the comprehension of summer mortalities that affects Crassostrea gigas Pacific cupped oysters' spa!. The aim is to characterize genetic families in a physiologic way, and to compare these families qualities with the noticed mortalities. The employed approach consists, on the one hand, in characterising Iivestocks qualities with instantaneous measurings of respiration and biometry. They respectively allow to estimate basic metabolism and to calculate state index. On the other hand, it consists in measuring differently degrees of resistance by the observation of respiration and mortality after thermal stresses. This study allowed to show that the environment and the stresses only act as indications of a pre-existent wealilless,...
Tipo: Text
Palavras-chave: Mortalité; Crassostrea gigas; Familles gééntiques; Métabolisme; Respiration; Mortality; Crassostrea gigas; Genetic family; Metabolism; Respiration.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00412/52321/53090.pdf

Divergence in physiological factors affecting swimming performance between anadromous and resident populations of brook charr     Salvelinus fontinalis
Provedor de dados: 5
Autores: Crespel, Amelie; Dupont-prinet, A.; Bernatchez, L.; Claireaux, Guy; Tremblay, R.; Audet, C.
In this study, an anadromous strain (L) and a freshwater-resident (R) strain of brook charr Salvelinus fontinalis as well as their reciprocal hybrids, were reared in a common environment and submitted to swimming tests combined with salinity challenges. The critical swimming speeds (Ucrit) of the different crosses were measured in both fresh (FW) and salt water (SW) and the variations in several physiological traits (osmotic, energetic and metabolic capacities) that are predicted to influence swimming performance were documented. Anadromous and resident fish reached the same Ucrit in both FW and SW, with Ucrit being 14% lower in SW compared with FW. The strains, however, seemed to use different underlying strategies: the anadromous strain relied on its...
Tipo: Text
Palavras-chave: Hybrids; Local adaptation; Metabolism; Swimming performance.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00376/48703/49288.pdf

Étude des voies de bioactivation du benzo[a]pyrène et du fluoranthène chez la sole commune (Solea solea) : Profil métabolique et génotoxicité
Provedor de dados: 5
Autores: Wessel, Nathalie.
The aim of this Ph‟D was to study the biotransformation and the genotoxicity of two PAH of interest, namely the benzo[a]pyrene (BaP) and the fluoranthene (Fluo), in the common sole Solea solea, which is a benthic species sensitive to chemical pollution. The use of in vitro exposure models and the application of two different chemical analytical techniques allowed demonstrating that, as in higher vertebrates, the dihydrodiols and the phenols of BaP and Fluo are the major metabolites produced by sole liver enzymes. In vitro, the genotoxic potential of BaP and Fluo was demonstrated in sole, by the production of DNA adducts and oxidative DNA damage. The genotoxicity of Fluo appeared to be 50 fold less important than the genotoxicity of BaP. In the case of BaP,...
Tipo: Text
Palavras-chave: Écotoxicologie; Hydrocarbures Aromatiques Polycycliques; Solea solea; Métabolisme; Génotoxicité; Ecotoxicology; Polycyclic Aromatic Hydrocarbons; Solea solea; Metabolism; Genotoxicity.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00032/14326/11609.pdf

Reduction du nitrate dans les sediments marins. Comparaison des activites mesurees in vitro et in situ
Provedor de dados: 5
Autores: Esteves, J; Bonin, P; Blanc, F; Mille, G; Bertrand, J.
In vitro nitrate reduction in hydrocarbon-polluted marine sediments has been studied by flow-through system. By analysing the rate of nitrate comsumption as a function of nitrate concentration the authors determined the Michaelis-Menten kinetics parameters. (km = 330 mu M; vax = 2.0 mu M/g super(-1)/h super(-1). The authors found a value of 60 mu M/m super(-2)/h super(-1) for the rate of in vitro nitrate consumption derived from the above kinetic parameters and from the nitrate concentration in the sediment. In parallel a value of 67.4 mu M/m super(-2)/h super(-1) was found for the rate of nitrate consumption under in situ conditions. The great similarity between both results would favor the in vitro technique as a valid and more convenient one.
Tipo: Text
Palavras-chave: MED; Biogeochemistry; Oil pollution; Methodology; Environmental conditions; Experimental research; Comparative studies; Metabolism; Bacteria; Sediment chemistry; Reduction; Nitrates; Denitrification; Nitrogen cycle.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-979.pdf

Associations between tissue fatty acid composition and physiological traits of performance and metabolism in the seabass (Dicentrarchus labrax)
Provedor de dados: 5
Autores: Chatelier, Aurelien; Mckenzie, David; Prinet, Aurelie; Galois, Robert; Robin, Jean; Zambonino, Jose-luis; Claireaux, Guy.
Seabass were fed for 4 months with diets where the lipid was provided as either canola oil (CO), palm oil ( PO) or fish oil (FO), to generate diversity in their tissue fatty acid (FA) composition and investigate how this influenced major traits of exercise performance, cardiac performance and respiratory metabolism. In particular, based upon previous observations, we investigated the hypothesis that enriching the fish tissues with oleic and linoleic acids (OA, 18:1n-9 and LA, 18:2n-6, respectively) from the CO and PO diets would improve maximum exercise and cardiac performance, and increase aerobic metabolic scope. This proved to be the case; exercise respirometry on bass fitted with cardiac flow probes revealed that those fed CO and PO diets had a...
Tipo: Text
Palavras-chave: Diet; Fatty acid; Cardiovascular performance; Metabolism; Swimming; Dicentrarchus labrax; Seabass.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1860.pdf

Deciphering the molecular adaptation of the king scallop (Pecten maximus) to heat stress using transcriptomics and proteomics
Provedor de dados: 5
Autores: Artigaud, Sebastien; Richard, Joelle; Thorne, Michael A. S.; Lavaud, Romain; Flye-sainte-marie, Jonathan; Jean, Fred; Peck, Lloyd S.; Clark, Melody S.; Pichereau, Vianney.
Background: The capacity of marine species to survive chronic heat stress underpins their ability to survive warming oceans as a result of climate change. In this study RNA-Seq and 2-DE proteomics were employed to decipher the molecular response of the sub-tidal bivalve Pecten maximus, to elevated temperatures. Results: Individuals were maintained at three different temperatures (15, 21 and 25 degrees C) for 56 days, representing control conditions, maximum environmental temperature and extreme warming, with individuals sampled at seven time points. The scallops thrived at 21 degrees C, but suffered a reduction in condition at 25 degrees C. RNA-Seq analyses produced 26,064 assembled contigs, of which 531 were differentially expressed, with putative...
Tipo: Text
Palavras-chave: Marine biology; Metabolism; DNA repair; Transcription regulation; Apoptosis; Energy reserves.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00301/41173/40360.pdf

Variations metaboliques des communautes bacteriennes lors d'une pollution par petrole brut, avec ou sans traitement
Provedor de dados: 5
Autores: Cauchi, B.
Metabolic potentialities of bacterial communities in marine water after Crude Oil pollution (Arabian Light) and in marine water after pollution and treatment (enrichment: Inipol EAP 22) have been studied and compared. With data analysis: regressions, hierarchical ascending classification and multifactor correspondence analysis, the treatment gives rise to conservation and one increase of enzymatic pool (second day) of the bacterial sample as a combined metabolic homogeneity contrary to pollution impact. The direct assimilation of hydrocarbons looks more regular and slow without treatment.
Tipo: Text
Palavras-chave: Bacteria; Data processing; Pollution effects; Crude oil; Marine pollution; Physiology; Metabolism; Microbiology.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1023.pdf

Metabolic substrates are not mobilized from the osmoregulatory organs (gills and kidney) of the estuarine pufferfishes Sphoeroides greeleyi and S. testudineus upon short-term salinity reduction
Provedor de dados: 83
Autores: Prodocimo,Viviane; Souza,Carine F.; Pessini,Cristina; Fernandes,Luiz Claudio; Freire,Carolina A.
The marine-estuarine species of pufferfishes Sphoeroides testudineus and S. greeleyi are very efficient osmoregulators. However, they differ with respect to their tolerance of salinity reduction. During low tide S. testudineus remains in diluted estuarine waters, whereas S. greeleyi returns to seawater (SW). The hypothesis tested here was that the short-term mobilization of metabolic substrates stored in their main osmoregulatory organs would correlate with this differential tolerance. Fishes exposed to 5‰ (for 6 h) were compared to those kept in 35‰. Branchial and renal contents of triglycerides, protein and glycogen were evaluated, and total ATPase activity accounted for the tissues' metabolism. Plasma osmolality, chloride and glucose, hematocrit, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish gill; Fish kidney; Glycogen; Metabolism; Osmoregulation; Tetraodontidae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000400009

Intermittent sustained swimming in 'matrinxã' Brycon amazonicus (Bryconidae: Bryconinae): hematological and metabolic responses
Provedor de dados: 83
Autores: Fabrizzi,Fernando; Moraes,Gilberto; Hackbarth,Araceli; Almeida,Luciana Cristina de; Arbeláez-Rojas,Gustavo; Nunes,Cleujosí da Silva.
In fish, studies on a wide variety of physiological effects of exercise have been reported since a long time. It has been attributed special attention to some types of exercise, however, its application as a healthful practice in the rearing and welfare of farming fish is rising in last few years. In this particular, long-term intermittent sustained swimming (ISS) has been not yet explored. In this work, the freshwater fish Brycon amazonicus was submitted to (ISS) for 30 days at velocity of 1.0 body-length sec-1 for 12h interspaced by 12h under still water. Hematology and metabolism were evaluated. Exercised fish decreased 30% the erythrocyte number and hemoglobin was unvaried. The stores of liver glycogen and muscular triacylglycerol (TAG) were increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adaptation; Exercise; Fish; Glycogen; Metabolism; Triacylglycerol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000200425

Temperature affects the hypoxia tolerance of neotropical Cichlid Geophagus brasiliensis
Provedor de dados: 83
Autores: Pereira,Jéssica A.; Veronez,Alexandra C.S.; Coppo,Gabriel C.; Duca,Charles; Chippari-Gomes,Adriana R.; Gomes,Levy C.
ABSTRACT Oxygen and temperature are the most limiting factors in aquatic environments. Several species are exposed to variations of these factors in water because of physical, chemical and biological processes. The objective of this study was to evaluate the metabolic profile and the tolerance to the hypoxia of Geophagus brasiliensis exposed to changes in temperature and oxygen availability. The fish were exposed to 20 and 90% of oxygen saturation combined with different temperatures (20°, 24° and 28° C) for 8 h. Hepatic and muscular glycogen, as well as the activities of lactate dehydrogenase (LDH), malate dehydrogenase (MDH), citrate synthase (CS) and their ratios were evaluated. Both glycogen and MDH activity showed a significant difference in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aerobic and anaerobic pathways; Behavior; Metabolism; Oxygen.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100202

Effect of salinity on survival, growth and biochemical parameters in juvenile Lebranch mullet Mugil liza (Perciformes: Mugilidae)
Provedor de dados: 83
Autores: Lisboa,Viviana; Barcarolli,Indianara Fernanda; Sampaio,Luís André; Bianchini,Adalto.
Teleost fish growth may be improved under isosmotic condition. Growth and metabolic performance of juvenile Mugil liza (isosmotic point: 12‰) were evaluated after 40 days in different salinities (0, 6, 12 and 24‰). Tests were performed in quadruplicate (30 fish/tank; 0.48 ± 0.1 g body weight; 3.27 ± 0.1 cm total length) under controlled water temperature (28.2 ± 0.1ºC) and oxygen content (&gt;90% saturation). Fish were fed on artificial diet (50% crude protein) four times a day until apparent satiation. Results showed that salinity influenced juvenile mullet growth. Fish reared at salinity 24‰ grew better than those maintained in freshwater (salinity 0‰). Gill Na+,K+-ATPase activity and whole body oxygen consumption showed an U-shape-type response over the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycogen; Growth; Metabolism; Na+; K+-ATPase.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000200447

Consumo de oxígeno, crecimiento y utilización del alimento durante el desarrollo larval del besugo Pagrus pagrus (Linné, 1758)(Pisces:Sparidae).
Provedor de dados: 20
Autores: Aristizabal, E.O.
Knowledge of the life history of fish larvae is of practical importance to understand variations in the survival rate not only for cultivated fish but also for fishery biology studies. With the aim of recognizing critical changes during the first days of life of red porgy larvae, the oxygen consumption, growth, and food utilization were studied. After hatching, the larvae were transferred to two 400 L fiberglass tanks with microalgae (Nannochloropsis oculata)and enriched rotifers (Brachionus plicatilis)as initial food. During the incubation and larval rearing, water temperature was kept at 18.0 ± 0.5ºC and salinity ranged from 33.0 to 34.0 psu. Results indicate a decrease in larval growth between day 3 and 4 after hatching, when larvae opened their mouths,...
Tipo: Journal Contribution
Palavras-chave: Fish culture; Marine fish; Fish larvae; Metabolism; Oxygen consumption; Fish culture; Metabolism; Fish larvae; Oxygen consumption; Http://aims.fao.org/aos/agrovoc/c_2918; Http://aims.fao.org/aos/agrovoc/c_4769; Http://aims.fao.org/aos/agrovoc/c_2924; Http://aims.fao.org/aos/agrovoc/c_5478.
Ano: 2006
URL: http://hdl.handle.net/1834/1557

Métabolisme benthique des herbiers du banc d'arguin (Mauritanie)
Provedor de dados: 20
Autores: Diagne, Ahmed.
Tipo: Theses and Dissertations
Palavras-chave: Biological oceanography; Metabolism; Benthic algae; Sea grass; Atlantic Ocean; Algae.
Ano: 2010
URL: http://hdl.handle.net/1834/4753

Redes tróficas y flujos de energía en comunidades de pozas de marea de Mar del Plata
Provedor de dados: 20
Autores: Fueyo Sanchez, L.
El estudio de la estructura de las redes tróficas reviste gran importancia en la comprensión de los ecosistemas por las relaciones que hay entre dicha estructura y las funciones ecosistémicas. Además ofrece una visión global de la distribución de las diferentes especies, sus interacciones y los flujos de energía que entre ellas se producen. Varios autores han observado que las redes tróficas presentan regularidades en su estructura (Briand y Cohen 1987, Cohen y Newman 1988, Pimm et al. 1991, Chen y Cohen 2001, Montoya y Solé 2003). Sin embargo, el cuerpo teórico existente hace hincapié en la descripción de la estructura de las redes y presta menos atención a su funcionamiento y a los flujos de materia y energía implicados, aunque algunos autores han hecho...
Tipo: Theses and Dissertations
Palavras-chave: Trophic relationships; Intertidal environment; Metabolism; Food webs; Intertidal environment; Metabolism; Food webs; Http://aims.fao.org/aos/agrovoc/c_3923; Http://aims.fao.org/aos/agrovoc/c_4769; Http://aims.fao.org/aos/agrovoc/c_10973.
Ano: 2008
URL: http://hdl.handle.net/1834/3879

Bioenergética del besugo Pagrus pagrus.
Provedor de dados: 20
Autores: Aristizabal, E.O.
The utilization of the energy of food by red porgy (Pagrus pagrus Linnaeus, 1758) from the Argentine Sea (SW Atlantic) was studied. From the energetic point of view, it is proposed that the strategy of patitioning, utilization and storage of the energy of food, is an indicator of the organism response to the environmental conditions. The main objective was to know the proportion of the energy of food derived to the different components of the bioenergetics budget. In order to estimate the monthly energy investment in reproductive growth, chemical analysis of gonads from fishes caught by the fishery fleet and from laboratory studies were performed. Two indexes were used to calculate the energy investment in female ovaries: the proportion of net daily energy...
Tipo: Theses and Dissertations
Palavras-chave: Fish culture; Bioenergetics; Eggs; Fish larvae; Food consumption; Energy budget; Metabolism; Oxygen consumption; Fish culture; Eggs; Food consumption; Metabolism; Fish larvae; Oxygen consumption; Http://aims.fao.org/aos/agrovoc/c_2918; Http://aims.fao.org/aos/agrovoc/c_2502; Http://aims.fao.org/aos/agrovoc/c_3016; Http://aims.fao.org/aos/agrovoc/c_4769; Http://aims.fao.org/aos/agrovoc/c_2924; Http://aims.fao.org/aos/agrovoc/c_5478.
Ano: 2003
URL: http://hdl.handle.net/1834/1466

Preliminary Observations on Livestock Productivity in Sheep Fed Exclusively on Haulms from Eleven Cultivars of Groundnut
Provedor de dados: 30
Autores: Blümmel, M.
Palavras-chave: Body weight; Groundnuts; Organic matter; Viruses; Aromatic compounds; Nitrogen; Germplasm; Metabolism; Quality; Biological phenomena.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3255

Stover fodder quality traits for dual-purpose sorghum genetic improvement
Provedor de dados: 30
Autores: Blümmel, M.
Palavras-chave: Nitrogen; Sorghum; Organic matter; Body weight; Bioreactors; Genetic improvement; Sampling; Aromatic compounds; Forecasting; Metabolism.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3197

The metabolic status of diapause embryos of &lt;i&gt;Artemia franciscana&lt;/i&gt; (SFB)
Provedor de dados: 27
Autores: Clegg, J.S.; Drinkwater, L.E.; Sorgeloos, P.
The brine shrimp &lt;i&gt;&lt;i&gt;Artemia&lt;/i&gt; franciscana&lt;/i&gt; is widely used in aquaculture and basic research in areas ranging from molecular biology to evolution and ecology. A key of its life history involves the production of encysted embryos whose development is halted (enters diapause) at the gastrula stage. These shelled embryos are released into the aqueous environment where diapause continues until terminated by suitable conditions that produce an "activated embryo", which then can resume development when conditions permit. Very little is known about the metabolism of diapause embryos, in contrast to activated embryos, which have been studied extensively. We have examined selected features of metabolism in diapause embryos produced in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diapause; Embryos; Metabolism; Artemia franciscana.
Ano: 1996
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2924

Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana
Provedor de dados: 86
Autores: Cangahuala-Inocente,Gabriela Claudia; Steiner,Neusa; Maldonado,Sara Beatriz; Guerra,Miguel Pedro.
The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid-Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total sugars; Starch; Pineapple guava; Metabolism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000300001

VERIFICATION OF THE MECHANISM OF GLYPHOSATE RESISTANCE IN ITALIAN RYEGRASS BIOTYPES
Provedor de dados: 87
Autores: VARGAS,L.; RUCHEL,Q.; AGOSTINETTO,D.; LAMEGO,F.P.; LANGARO,A.C.; PIESANTI,S.R.
ABSTRACT The intense use of glyphosate for weed control led to the emergence of several cases of resistance to this herbicide. Weeds can survive the application of herbicides due to several factors, which may or may not be related to the herbicide site of action. The objectives of this study were to quantify the accumulation of shikimate in ryegrass biotypes in response to glyphosate application; investigate possible mutations on the EPSPs gene in susceptible and resistant biotypes; and evaluate the response of ryegrass biotypes to the application of glyphosate after treatment with a metabolism inhibitor of cyt P450 monooxygenase. The seeds of ryegrass biotypes with suspected resistance came from the municipality of São Valentim, RS (SVA 1 and SVA 4) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shikimic acid; EPSPs; Lolium multiflorum; Metabolism.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300565

Digestible Threonine Levels in the Starter Diet of Broilers Derived from Breeders of Different Ages
Provedor de dados: 89
Autores: Tanure,CBGS; Santos,JS; Oliveira,EM; Laboissiere,M; Racanicci,AMC; Mc Manus,CM; Café,MB; Stringhini,JH.
ABSTRACT The aim of this study was to evaluate the effect of digestible threonine supplementation in the starter diet on the performance, intestinal parameters, and nutrient metabolism of broilers derived from breeders of different ages. In total, 480 one-day-old Cobb chicks, derived from 38-or 49-week-oldbreeders, were housed in experimental battery cages until 21 days of age and fed four different threonine levels (800, 900, 1,000, or 1,100 mg/kg) in the starter feed. A completely randomized experimental design in a 2x4 factorial arrangement (breeder age x threonine levels) was applied, totaling eight treatments with five replicates of 12 birds each. Broilers from older breeders fed 800 mg digestible threonine/kg of diet presented higher weight gain,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Development; Metabolism; Nutrition; Poultry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015001000031

Effect of an Enzymatic Blend and Yeast on the Performance, Carcass Yield and Histomorphometry of the Small Intestine in Broilers from 21 to 42 Days of Age
Provedor de dados: 89
Autores: Sousa,RF; Dourado,LRB; Lopes,JB; Fernandes,ML; Kato,RK; Nascimento,DCN; Sakomura,NK; Lima,SBP; Ferreira,GJBC.
ABSTRACT The present study was conducted to evaluate the performance, carcass yield, and histomorphometry of the small intestine of broilers fed yeast sugarcane supplemented with enzymatic blend from 22 to 42 days of age. Seven hundred broiler chicks were distributed in a completely randomized design in a factorial design (2x3+1), two levels of the enzyme blend (0 and 200 g/ton), three protein levels (0%, 6% and 12%) and a control diet. The level of 6% yeast showed higher feed intake and weight gain after 33 days. From 22 to 42 days there was no significant effect (p&lt;0.05) to the use of yeast in animal performance, carcass yield and cuts. At 42 days an interaction between the factors for width and crypt depth in the duodenum and jejunum, respectively,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cane sugar; Microscopy; Metabolism; Saccharomyces cerevisiae; Xylanase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200300

Feeding high-moisture corn grain silage to broilers fed alternative diets and maintained at different environmental temperatures
Provedor de dados: 89
Autores: Cruz-Polycarpo,VC; Sartori,JR; Gonçalves,JC; Pinheiro,DF; Madeira,LA; Polycarpo,GV; Zanetti,LH; Santos,TS; Pezzato,AC.
The effects of the dietary substitution of dry corn by high-moisture corn grain silage (HMCGS) were evaluated on the performance, nutrient digestibility and serum biochemical parameters of broilers reared in an alternative production system and submitted to different environmental temperatures. A total of 288 one-day-old male Cobb chicks were distributed according to a randomized block design in a 3x4 factorial arrangement: three environmental temperatures (hot, thermoneutral or cold) and four levels of HMCGS in substitution of dry corn (0%, 20%, 40% or 60%). The acid analysis showed that the evaluated HMCGS contained average percentage values of ethanol, lactic acid, and acetic acid (expressed in 100% of dry matter) of 0.7690, 2.7320 and 0.0249%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Absorption; Alternative feeds; Poultry; Cholesterol; Metabolism; Triglycerides.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000400017

Determination of apparent metabolizable energy of crude glycerin in broilers chickens
Provedor de dados: 89
Autores: Gianfelici,MF; Ribeiro,AML; Penz Jr,AM; Kessler,AM; Vieira,MM; Machinsky,T.
The objective of the experiment was to determine the Apparent Metabolizable Energy corrected for nitrogen (AMEn) of a glycerin product derived from biodiesel production for broilers. A number of 35 male Ross 308 broilers, with 35 days of age was fed ad libitum diets containing 0, 5, 10, 15, or 20% glycerin. AMEn was determined using total excreta collection, with four days of adaptation and three days of collection. Increasing glycerin intakes resulted in increased water excretion. Glycerin AMEn varied as a function of dietary inclusion levels, and a cubic equation was identified by regression analysis. Maximum AMEn level- 4890 kcal/kg DM- was obtained with 15% glycerin inclusion. However, this level caused excessive water excretion, making its use...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycerol; Metabolism; Water excretion.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2011000400006

Comparison of the effects of semi-refined rice oil and soybean oil on meat oxidative stability, carcass yield, metabolism, and performance of broilers
Provedor de dados: 89
Autores: Moraes,ML de; Ribeiro,AML; Kessler,A de M; Cortés,MM; Ledur,VS; Cura,E.
Two experiments (EXP 1 and EXP 2) were conducted to compare soybean oil (SO) and semi-refined rice oil (RBO) added to broilers diets. In EXP 1, 400 male Ross x Ross 308 broilers were reared in battery cages, and their performance was evaluated. A metabolism assay was performed. In EXP 2, 1344 broilers from the same strain were reared in floor pens with rice husks litter. In addition to performance, carcass yield and meat oxidative stability were evaluated. In both EXP, birds were distributed in a 2x4 factorial arrangement, with two types of oils (SO or RBO) and four oil inclusion levels (1%, 2.5%, 4%, or 5.5%). Two periods were considered: starter (1 to 21 days of age) and grower (22 to 42 days). In both EXP, oil type had no influence on starter...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler; Carcass; Metabolism; Rice oil; Soybean oil; Performance.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300004

Effect of dietary fiber, genetic strain and age on the digestive metabolism of broiler chickens
Provedor de dados: 89
Autores: Krás,RV; Kessler,A de M; Ribeiro,AML; Henn,JD; Bockor,L; Sbrissia,AF.
In this study, 360 male broilers, out of which 240 of a fast-growing strain (Cobb500), and 120 of a slow-growing strain (Label Rouge), were used to evaluate the effect of dietary fiber on digesta transit time and digestive metabolism during the period of 1 to 42 days of age. A completely randomized experimental design with a 3x2 factorial arrangement was applied, consisting of three groups of birds (slow-growing - SG; fast-growing fed ad libitum - FGAL; and fast-growing pair-fed with SG broilers - FGPF) and two iso-protein diets (a 3100 kcal ME/kg low-fiber diet - LFD- and a 2800 kcal ME/kg high-fiber diet - HFD- with 14% wheat bran and 4% oat hulls). HFD-fed birds presented lower ME retention (p &lt; 0.001) and lower dry matter metabolizability (DMM) (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Age; Fiber; Metabolism; Pair-fed; Strain.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000200003

Effect of an Enzyme Blend on the Performance, Diet Metabolizability, Phosphorous Retention, and Bone Mineralization of Broilers Fed Diets Containing Defatted Rice Bran
Provedor de dados: 89
Autores: Moraes,ML; Ledur,VS; Kessler,AM; Machado,PHM; Della,MP; Ribeiro,AML.
An experiment was conducted to evaluate the effect of an enzyme blend (EB) on the performance, diet metabolizability, phosphorus (P) retention, and bone mineralization of broilers fed diets containing 10% defatted rice bran (DRB). In total, 432 one- to 38-d-old male Cobb broilers were evaluated according to a completely randomized experimental design in 3 x 2 factorial arrangement. Three diets were tested with two nutrient reductions (NR) in the matrix (standard diet; NR I of 75 kcal/kg ME, 0.1% Ca and 0.1% available P; and NR II of 100 kcal/kg ME, 0.1% Ca and 0.1% available P) with or without the addition of an EB (200 g/t). The coefficients of total tract apparent retention (CTTAR) of the diets and P retention were determined by collecting excreta during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler chicken; Defatted rice bran; Enzyme blend; Metabolism; Mineral retention; Performance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000200191

PERFORMANCE AND NUTRIENT METABOLIZABILITY IN BROILERS FED DIETS CONTAINING CORN CONTAMINATED WITH FUMONISIN B1 AND ESTERIFIED GLUCOMANNAN
Provedor de dados: 89
Autores: Oliveira,EM; Tanure,CBGS; Castejon,FV; Castro,RMAD; Rocha,FRT; Carvalho,FB; Andrade,MA; Stringhini,JH.
ABSTRACTAn experiment was conducted to evaluate the performance and nutrient metabolizability of broilers fed diets containing fumonisin B1 (FB1) and an esterified glucomannan (EGM). In total, 420 male broilers were distributed according to a 3 x 2 + 1 factorial arrangement, corresponding to three FB1 exposure times (seven, 21, or 35 days), two dietary glucomannan addition levels (0 or 0.1% EGM), and control diet, totaling seven treatments. The following diets were fed: 1) Control diet, 2) pre-starter diet containing FB1, 3) pre-starter diet containing FB1 and 0.1% EGM, 4) starter diet containing FB1, 5) starter diet containing FB1 and 0.1% EGM, 6) grower diet containing FB1, and 7) grower diet containing FB1 and 0.1% EGM. On d 7, broilers fed FB1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adsorbent; Fungi; Grains; Metabolism; Nutrition; Poultry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000300313

Foliar carbohydrates content and invertase activity in vines at São Francisco River Valley - Brazil
Provedor de dados: 94
Autores: Dantas,Barbara França; Ribeiro,Luciana de Sá; Silva,Alexandro Pereira da; Luz,Sara Raquel de Souza.
The irrigated agriculture at the São Francisco River Valley, Northeast Brazil, shows an increasing production of grapes for winery. Among the wines produced there the one obtained from Vitis vinifera L., cultivar Syrah, stands out due to its adaptation to the climatic conditions of the region. However, little is known about carbohydrates metabolism of vines cultivated in this region. The objective of this work was to evaluate sugar and starch contents and the invertase activity in vines leaves during two consecutive growing seasons. The experiment was carried out at Embrapa Semi-Árido and at Santa Maria Winery, respectively located in Petrolina and Lagoa Grande, Pernambuco-Brazil. Leaves were collected weekly from January to December of 2003 and assessed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grape; Metabolism; Sugar.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452005000200004

Residues of carbosulfan and its carbofuran metabolites and 3-hydroxy-carbofuran in oranges
Provedor de dados: 94
Autores: Trevisan,Marcos José; Baptista,Gilberto Casadei de; Trevizan,Luiz Roberto Pimentel; Papa,Geraldo.
The objectives of this study were to evaluate the residues of the insecticide carbosulfan and its carbofuran metabolites and 3-hydroxy-carbofuran in orange compartments (whole fruit, bagasse and juice) and comparison between the residual levels found in fruits with the maximum residue level and the safety interval established by the Brazilian legislation. Two field experiments were carried out, both with the following treatments: a-check; b-one application of 10 g of carbosulfan . 100 L-1 of water; c-one application with twice the rate applied in treatment b; d-four applications with the same rate applied in treatment b. Samples were taken at (-1), zero, 1, 3, 7, 14, 21 and 28 days after the last or unique application. The quantitative determinations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus sinensis; Metabolism; Pesticide residues in citrus; Maximum residue level; Safety interval.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452004000200012

Meal size effects on the postprandial metabolic response of Bothrops alternatus (Serpentes: Viperidae)
Provedor de dados: 103
Autores: Gavira,Rodrigo S. B.; Andrade,Denis V.
We examined the effects of meal size on the postprandial metabolic response of the lancehead Bothrops alternatus (Duméril, Bibron &amp;amp; Duméril, 1894), fed mice equaling to 5, 10, 20, and 40% of the snake's body mass. The maximum O2 consumption rates measured during digestion increased with meal size, reaching levels up to 2.8-7.8-fold higher than the metabolic rate measured during fasting. Specific Dynamic Action (SDA) duration also increased with meal size, lasting from 54 to 212 hours to complete. Under our experimental conditions, 30ºC, the majority of our snakes failed to completely digest prey with a relative size of 40%. The SDA coefficient ranged from 17 to 27% of the energy content of the meal and was not affected by meal size.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolism; Postprandial; Prey size; Snakes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702013000300005

Metabolic, productive and reproductive responses to postpartum short-term supplementation in primiparous beef cows
Provedor de dados: 96
Autores: Astessiano,Ana Laura; Pérez-Clariget,Raquel; Espasandín,Ana Carolina; López-Mazz,Carlos; Soca,Pablo; Carriquiry,Mariana.
The objective of this study was to evaluate the effect of a short-term supplementation with rice bran (2 kg/cows/day) on the endocrine and metabolic profiles and hepatic gene expression, associated with the reproductive response in beef cows in grazing conditions. Thirty-eight primiparous beef cows (Hereford, Angus and Hereford × Angus) were used in a randomized block design according to genotype, calving date and body condition score at calving (BCS). At 64±14 days postpartum (day 0 of the experiment), cows were assigned to two nutritional treatments: i) control, grazing native pastures (CON, n=19) and ii) supplemented (SUP, n = 19) for 21 days (days 1 to 21 of the experiment). Blood samples were collected at 0, 7 and 22 days and liver biopsies were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GH-IGFI axis; Metabolism; MRNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000400003

Prediction equations for energy values of animal meals obtained using meta-analysis
Provedor de dados: 96
Autores: Oliveira,Camilla Roana Costa de; Rabello,Carlos Bôa-Viagem; Souza,Janete Gouveia de; Nascimento,Guilherme Rodrigues do; Lopes,Elainy Cristina; Silva,Almir Ferreira da; Silva,Jaqueline de Cássia Ramos da; Macambira,Gabriel Miranda.
ABSTRACT The objective of this study was to determine prediction equations to estimate the nitrogen-corrected apparent metabolizable energy (AMEn) values of animal meals used in broiler diets through meta-analysis. A bibliographic review was undertaken with studies conducted in Brazil from 2000 to 2016 to catalogue information on AMEn values and the following chemical elements in the composition of the feedstuffs: crude protein (CP), ether extract (EE), gross energy (GE), mineral matter (MM), calcium (Ca), and phosphorus (P). Groups were also catalogued and formed according to sex and age of birds. Chemical correlations were analyzed, and a multiple linear regression model with the stepwise procedure was used to examine the association between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AMEn; Broiler; Meat and bone meal; Metabolism; Prediction.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100537

Effect of pectin extracted from citrus pulp on digesta characteristics and nutrient digestibility in broilers chickens
Provedor de dados: 96
Autores: Silva,Vanessa Karla; Morita,Viviane de Souza; Boleli,Isabel Cristina.
The experiment was carried out to evaluate the effects of continuous ingestion of pectin on intestinal viscosity, intestinal transit time, excreta moisture content, nutrient digestibility and energy metabolism of broilers at starter and growth phases. We used 240 one-day-old Cobb male broiler chicks, distributed in a completely randomized experimental design. Treatments consisted of four concentrations of pectin (0, 10, 30 and 50 g kg-1) with six replicates of 10 birds each. The ingestion of pectin supplied in the feed by broilers at the starter phase increased intestinal viscosity and intestinal transit time, reduced excreta moisture, improved the use of apparent metabolizable energy, nitrogen-corrected apparent metabolizable energy, coefficient of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Development; Feed additive; Metabolism; Soluble fiber; Viscosity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000800007

Urea in sugarcane-based diets for dairy cows
Provedor de dados: 96
Autores: Santiago,Alberto Magno Ferreira; Campos,José Maurício de Souza; Oliveira,André Soares de; Valadares Filho,Sebastião de Campos; Santos,Stefanie Alvarenga; Souza,Shirley Motta de; Santiago,Iara Furtado.
We evaluated the effect of adding four levels (0, 4, 8 and 12 g/kg, as fed) of a mixture (9:1) of urea and ammonium sulfate (UAs) to sugarcane on feed intake and digestibility, productive performance and metabolism of nitrogen compounds of dairy cows. Twelve multiparous Holstein cows (12.6±0.5 kg/d of milk, 225±90 days in milk) were distributed in three 4 × 4 Latin squares, receiving diets with the same amount of nitrogen (125 g crude protein/kg of dry matter). Concentrate feed was supplied at a ratio of 1 kg for each 3 kg of milk produced. The sugarcane presented 21.9 ºBrix. The level of UAs did not affect intake, total digestibility of diet components, milk production or milk components. Increasing UAs level linearly increased concentration of plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolism; Milk production; Nitrogen.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000600010

Antioxidants and dairy production: the example of flax
Provedor de dados: 96
Autores: Petit,Hélène V.
This manuscript reports on the main problems decreasing productivity of dairy cows (e.g. fatty liver syndrome and poor fertility) and how antioxidants could enhance it. High producing dairy cows are prone to oxidative stress, and the situation can be exacerbated under certain environmental, physiological, and dietary conditions. Antioxidants have important effects on the expression of genes involved in the antioxidant status, which may enhance animal health and reproduction. Moreover, antioxidants may contribute to decrease the incidence of spontaneous oxidized flavour in milk enriched in polyunsaturated fatty acids. Plant lignans are strong antioxidants and flax is the richest source of plant lignans. Flax lignans are converted in the mammalian lignans...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Bovine; Flaxseed; Metabolism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300035

Energy utilization of light and heavy weaned piglets subjected to different dietary energy levels
Provedor de dados: 96
Autores: Ribeiro,Andréa Machado Leal; Farina,Giovani; Vieira,Marcia de Souza; Perales,Valentino Arnaiz; Kessler,Alexandre de Mello.
ABSTRACT This study was conducted to evaluate the effects of dietary metabolisable energy (ME: 3.25, 3.40, 3.55, or 3.70 Mcal kg−1) and weaning weight (WW: light 4.0±0.7 kg, and heavy: 6.3±0.6 kg) on productive response and energy utilization of weaned piglets. Sixty-four male piglets were housed in 32 metabolic cages (two animals per cage) during the first 14 d postweaning. At day 15, only one animal per cage was kept until day 28. Body composition, energy, and nutrient deposition rates and energy utilization efficiency were measured through a comparative slaughter procedure. Piglets with light WW had a poorer feed conversion ratio and lower weight gain and feed intake when expressed per live weight. Increased ME led to greater daily fat deposition in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolism; Pig nutrition; Protein:energy ratio; Tissue deposition.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000900532

Insulin addition to swine semen diluted and cooled at 15 ºC
Provedor de dados: 96
Autores: Cunha,Evandro César Pereira; Zangeronimo,Márcio Gilberto; Murgas,Luis David Solis; Braga,Douglas Evangelista; Pereira,Bárbara Azevedo; Rocha,Luiz Gustavo Pessoa; Faria,Bruno Generoso; Pereira,Luciano José.
The objective of this study was to evaluate the effect of adding different doses of insulin to swine semen processed and stored at 15 ºC. The experiment used sixteen ejaculates from four commercial breeding pigs, distributed in a randomized block design (ejaculate) with split plot along time (0, 24, 48 and 72 hours of storage) with four treatments (insulin levels - 0.0 4.0 8.0 and 12.0 IU per dose) and 16 repetitions. The experimental unit was made of two insemination doses of 100 mL each, with 3×10(9) spermatozoids. Insulin used was NPH-human, added at the time of processing the doses. The addition of insulin did not affect motility, sperm viability, the percentage of abnormal cells, the osmotic resistance or the degradation rate of motility in 120...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artificial insemination; Boar; Hormone; Metabolism; Sperm; Reproduction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000400031

Chemical composition and energy value of guava and tomato wastes for broilers chickens at different ages
Provedor de dados: 96
Autores: Lira,Rosa Cavalcante; Rabello,Carlos Bôa-Viagem; Silva,Edney Pereira da; Ferreira,Paulo Vanderlei; Ludke,Maria do Carmo Mohaupt Marques; Costa,Edgenes Vital.
The chemical composition and energy value of guava and tomato wastes for broilers at different ages were determined in this research. The metabolism assays were carried out by using the methodology of total excreta collection to calculate the chemical composition of wastes collected in different months. A total of 270 COBB broiler chicks was used: 150 in the period from 1 to 8 days of age (phase 1) and 120 chicks were used in the period from 10 to 17 days of age (phase 2). The trials were analyzed as completely randomized design with three treatments with five replications of 10 and 8 birds at the respective ages. The treatments consisted of different diets: one reference diet, one with 30% tomato meal and another with 20% guava meal, both partially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative food; Chemical composition; Metabolism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000500012

Evaluation of blood metabolites in dairy cows grazing under two pasture allowances and supplemented with corn silage under restricted grazing conditions
Provedor de dados: 96
Autores: Morales,Álvaro; Grob,Daniela; Wittwer,Fernando; Müller,Ananda; Balocchi,Óscar; Pulido,Rubén.
ABSTRACT This study was conducted to evaluate the influence of management tools - daily pasture allowance and corn silage supplementation - during periods of forage shortage on the metabolism of dairy cows grazing low-mass pasture in temperate regions. Forty lactating Holstein cows were used during an experimental period of seventy days (April 15 to June 23, 2012). Blood metabolites and milk production were determined in fall-calving dairy cows grazing under two daily pasture allowances (PA) (moderate, 17 kg vs. high, 25 kg dry matter (DM)) and supplemented with corn silage (CS) (low, 4.5 kg vs. high, 9.0 kg DM). All cows received 3 kg DM of concentrate. Plasma concentrations of β-hydroxybutyrate (BHBA), non-esterified fatty acids (NEFA), and urea were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cow; Grazing system; Metabolism; Restricted feeding; Supplementation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016001100686

Live weight and metabolic hormone profile in steers moved in drives
Provedor de dados: 96
Autores: D'Oliveira,Marcella Cândia; Souza,Maria Inês Lenz; Ítavo,Camila Celeste Brandão Ferreira; Corrêa Filho,Ruy Alberto Caetano; Morais,Maria da Graça; Negrão,João Alberto; Franco,Gumercindo Loriano.
The objective was to determine the effects of driving on the growth of steers during 55 days of moving by drive and 84 days after the arrival. Twenty-five steers were randomly chosen from a herd of 1,000 Nellore cattle, which were evaluated in two periods: the driving period, in which animals were moved a distance of 700 km on foot for 55 d; and the post-driving period, which lasted 84 d. Serum samples were obtained for hormone dosing (T3, T4, cortisol, and IGF-I) by radioimmunoassay using commercial kits. The data was collected at different time points during the experiment. No changes in live weight were observed between days 0 and 55. During the post-driving period, on the 28th, 56th, and 84th d, the average weights were 226.10 kg, 224.28 kg, and 242.6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compensatory gain; Metabolism; Post-driving; Transportation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014001000551

Energetic and nutrient metabolizability values of corn obtained with nutritional corrections for broilers
Provedor de dados: 96
Autores: Makiyama,Letícia; Alvarenga,Renata Ribeiro; Rodrigues,Paulo Borges; Zangeronimo,Márcio Gilberto; Oliveira,Evelyn Cristina de; Bernardino,Verônica Maria Pereira; Garcia Júnior,Antônio Amandio Pinto; Bertechini,Antônio Gilberto.
The experiment was carried out to evaluate the influence of nutritional correction on the partial diet replacement or pure-food methodology to determine the energy values (apparent metabolizable energy - AME and nitrogen-correct apparent metabolizable energy - AMEn) and coefficient of metabolizability of nutrients of maize. The method of total excreta collection was used, with 120 female Cobb 500® broilers weighing 1,339±3 g and at 28 days of age distributed in a completely randomized design with five replicates and four birds per experimental unit. The treatments were pure maize with three levels of nutritional correction (0, 50 and 100%) and a maize-replaced basal diet (400 g/kg) with two levels of correction (0 and 100%). The corrections were made in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolism; Metabolizable energy; Replacement methodology; Total collection.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000500031

Metabolic and histologic responses of pacu (Piaractus mesopotamicus) fed diets supplemented with increasing concentrations of ractopamine
Provedor de dados: 96
Autores: Drumond,Mariana Martins; Naves,Luciana de Paula; Ribeiro,Paula Adriane Perez; Oliveira,Marinez Moraes de; Okamura,Daniel; Resende,Anaise Emanuele; Cantarelli,Vinicius de Souza; Rosa,Priscila Vieira.
ABSTRACT An experiment was conducted during 60 days with forty pacu males fed diets supplemented with increasing concentrations of ractopamine (0.00, 11.25, 22.50, 33.75, and 45.00 mg kg−1). Eight fish were evaluated for each experimental diet. Performance and survival rate of the fish were measured. At the end of the experiment, blood was collected to determine the levels of cortisol, triacylglycerol, and protein. Moreover, the liver was collected to determine the activities of glucose-6-phosphate dehydrogenase and malic enzymes. The fillets were collected to determine chemical composition, and histologic cuts were analyzed to verify muscle growth and deposition of adipose tissue between muscle fibers. Increasing concentrations of dietary ractopamine did...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta-adrenergic agonist; Enzyme; Metabolism; Stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100100

Intake, nutrient apparent digestibility and ruminal constituents of sheep fed diets with canola, sunflower or castor oils
Provedor de dados: 96
Autores: Maia,Michelle de Oliveira; Susin,Ivanete; Ferreira,Evandro Maia; Nolli,Cristine Paduan; Gentil,Renato Shinkai; Pires,Alexandre Vaz; Mourão,Gerson Barreto.
The objective in this experiment was to determine the effects of feeding diets with canola, sunflower or castor oils on intake, nutrient apparent digestibility and ruminal constituents of crossbred Dorper × Santa Inês sheep. Four rumen-cannulated animals of 90.2±11.4 kg average body weight were assigned to a 4 × 4 latin square. Animals remained individually in cages for the metabolism assay and were fed diets containing roughage at 500 g/kg and concentrate based on ground corn and soybean meal also at 500 g/kg. No oil was added to the control diet, whereas the others had canola, sunflower or castor oils at 30 g/kg (DM basis). There was no difference for the intake of DM and nutrients, except for ether extract, which was greater when animals received oil....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammonia; Lipids; Metabolism; Short-chain fatty acids.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001100008

Energetic metabolism in fasting sheep: regularization of metabolic profile by treatment with oral glucose, with prior handling of gastric groove
Provedor de dados: 96
Autores: Martín-Alonso,María José; Escalera-Valente,Francisco; Cal-Pereyra,Luis G.; Benech,Alejandro; Alonso,María Pilar; González-Montaña,José Ramiro.
ABSTRACT The objective of this research was to evaluate a possible corrective measure against negative metabolic states, as occurs in the advanced stage of gestation in ewes, and that sometimes produces a disease called pregnancy toxaemia. In the present research, we found that the joint administration of i.v. lysine-vasopressin (0.08 IU/kg body weight, BW) and an oral glucose solution (50 g) produces an increase in blood glucose, which persists for some time (up to 6 h); therefore, it could be used in the treatment of pregnancy toxaemia. This therapy is based on the fact that lysine-vasopressin induces gastric groove closure in adult ruminants, enabling orally administered glucose to reach the abomasum directly, from where it rapidly passes into the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ewes; Gastric groove; Glycaemia; Metabolism; Pregnancy; Toxaemia; Vasopressin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100723

Physiological responses of growing pigs to high ambient temperature and/or inflammatory challenges
Provedor de dados: 96
Autores: Campos,Paulo Henrique Reis Furtado; Le Floc'h,Nathalie; Noblet,Jean; Renaudeau,David.
ABSTRACT Global warming is one of the major environmental threats facing the world in the 21st century. This fact will have a significant impact on pig production due to its direct effects on welfare, health, and performance of pigs. Besides, the effects of high temperatures will presumably become more important over the next decades due to the development of pig production in developing countries mainly located in tropical and subtropical areas, where animals are often exposed to ambient temperatures above their thermal comfort zone. Furthermore, pigs reared in tropical areas are often confronted to sanitary challenges including poor hygiene conditions, lack of respect for sanitary rules, and pathogens. This results in the stimulation of the immune system...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal biometereology; Heat stress; Metabolism; Physiology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000600537

Growth performance and metabolic response of Nile tilapia fed rations supplemented with autolized yeast and zinc
Provedor de dados: 96
Autores: Signor,Altevir; Pezzato,Luiz Edivaldo; Padilha,Pedro de Magalhães; Padovani,Carlos Roberto; Barros,Margarida Maria.
This research evaluated the influence of yeast and zinc on growth performance and metabolic responses of Nile tilapia. Diets were formulated to contain 32.0% digestible protein and 3,240 kcal DE/kg diet and the following autolized yeast (%):zinc (mg/kg)relationships: 0.0:0.0; 0.0:79.5; 2.0:0.0; 0.795:79.5; 2.0:200; 4.0:400; 6.0:600; 12.0:1,200 and 14.0:1,400. It was used 135 fingerlings (7.27 ± 0.19 g), distributed in a complete random design in 27 50-L aquaria and they were fed ad libitum four times a day for 128 days. It was evaluated growth performance and metabolic responses, weight gain, apparent feed conversion; protein efficiency and survival rate; percentage of protein, ether extract, dry weight and ashes in the muscle and in the bones; ammonia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fingerlings; Growth; Metabolism; Metabolites; Minerals.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001200002

Residual feed intake and hematological and metabolic blood profiles of lle de France lambs
Provedor de dados: 96
Autores: Paula,Edson Ferraz Evaristo de; Souza,Damaris Ferreira de; Monteiro,Alda Lúcia Gomes; Santana,Miguel Henrique de Almeida; Gilaverte,Susana; Rossi Junior,Paulo; Locatelli Dittrich,Rosangela.
The objectives of this study were to estimate the phenotypic correlations of residual feed intake (RFI) and gross feed efficiency (GFE) with hematological and metabolic blood profiles of lambs and to determine the differences for these traits in animals of different RFI classes. Twenty Ile de France male lambs, 115±8 days of age and 31.3±4.1 kg of body weight (means ± SD), were individually housed and their dry matter intake was measured over 65 days. They were weighed every 13 days to determine the average daily weight gain and two blood samples were collected at the last two weighings (at 07h30) for analysis of blood variables. The animals were divided into two classes: negative RFI (most efficient: &lt;0.5 SD below the mean; n = 6) and positive RFI...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood biochemistry; Feed efficiency; Hematology; Metabolism; Sheep.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013001100007

Specific fatty acids as metabolic modulators in the dairy cow
Provedor de dados: 96
Autores: Pires,J.A.A.; Grummer,R.R.
This review summarizes recent developments on the utilization of specific fatty acids to modulate bovine energy metabolism, with emphasis on the periparturient dairy cow. A number of experiments have assessed the effects of polyunsaturated fatty acids on bovine hepatic energy metabolism using in vitro and in vivo models. Treatment of hepatocytes with specific fatty acids altered energy metabolism in vitro. For example, linolenic acid seemed to decrease hepatocyte triacylglycerol accumulation. This effect was confirmed in vivo, using parenteral infusions of emulsions derived from different fat sources to feed-restricted non-lactating cows. Additionally, polyunsaturated fatty acids can increase whole body response to insulin, potentially enhancing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; CLA; Linseed; Fish oil; Metabolism; Polyunsaturated fatty acid.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982008001300033

Phytogenic additives and organic acids in broiler chicken diets
Provedor de dados: 96
Autores: Fascina,Vitor Barbosa; Sartori,José Roberto; Gonzales,Elisabeth; Carvalho,Fabyola Barros de; Souza,Ivan Mailinch Gonçalves Pereira de; Polycarpo,Gustavo do Valle; Stradiotti,Ana Cristina; Pelícia,Vanessa Cristina.
The experiment evaluated the influence of isolated or associated phytogenic additives (PA) and organic acids (OA) on nutrient digestibility, performance and carcass characteristics of broiler chickens. Two experiments were conducted in a completely randomized design with a 2 × 2 + 1 factorial arrangement of treatments (with or without PA × with or without OA + antibiotic performance enhancer and anticoccidial). In the first experiment, two metabolic tests were conducted to determine the metabolizability coefficients of the nutrients of starter and growth diets. In the second experiment, 2520 one-day-old chicks were housed in 40 experimental units to evaluate the performance and carcass characteristics. The phytogenic additives and organic acids, isolated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acidifier; Additive; Antibiotics; Herbal extract; Metabolism; Sanitary challenge.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000008

New approaches, development, and improvement of methodologies for the assessment of B-vitamin requirements in dairy cows
Provedor de dados: 96
Autores: Girard,Christiane L.
ABSTRACT Studies on B-vitamin requirements of cattle, conducted more than 60 years ago, concluded that mature ruminants do not require B-vitamin supplements because the amounts of vitamins provided by the diet and synthesized by the ruminal microflora were sufficient to prevent emergence of deficiency symptoms. As a result, the impact of subclinical deficiency on maintenance of normal and efficient metabolism has been disregarded and very little research effort has been devoted at defining dairy cow requirements for B vitamins. However, emergence of deficiency symptoms is the last stage of the deficiency; deficiency appears as soon as the supply is inferior to the needs, leading to a loss of metabolic efficiency. As B vitamins play critical roles in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B-vitamin; Dairy cow; Metabolism; Requirement; Rumen.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000700614

Nutrient reduction in rations with phytase for growing pigs
Provedor de dados: 96
Autores: Rodrigues,Valéria Vânia; Cantarelli,Vinícius de Souza; Amaral,Nikolas de Oliveira; Zangeronimo,Márcio Gilberto; Brito,Jeronimo Ávito Gonçalves de; Fialho,Elias Tadeu.
Two experiments were conducted to evaluate the performance, the energy and nutrient balance and economic viability of production of growing pigs fed diets with reduced levels and phytase. In experiment 1, 60 barrows and gilts (initial weight 25 ± 1.72 kg) were housed in a randomized block design with three diets, ten replicates and two animals per experimental unit (one male and one female). One of the diets was formulated with a reduced level with phytase and evaluated in comparison to a positive-control diet that was formulated according to the nutritional recommendations for animals of this breed, and a negative-control diet without phytase. The different diets did not affect feed intake, but the diet with phytase promoted better final weight, daily...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diet formulation; Enzyme; Metabolism; Nutrition; Performance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000200019

Diet-induced developmental plasticity in life histories and energy metabolism in a beetle
Provedor de dados: 97
Autores: URREJOLA,SERGIO; NESPOLO,ROBERTO; LARDIES,MARCO A.
Adaptive phenotypic plasticity, has been recognized as an important strategy by which organisms maximize fitness in variable environments, which vary through development. A disassociation among stages should represent a null effect of the environment experienced during early ontogeny in the expression of adult traits. Food quality greatly influences survival, development and reproduction in many arthropod herbivores. We examined the effects of diet protein in physiological and life-history traits in the yellow mealworm beetle Tenebrio molitor through ontogeny. We established four experimental treatments: Low Protein (LP), Low Protein Control (LPC), High Protein (HP), and High Protein Control (HPC) with recently eclosioned larvae each. Individuals were...
Tipo: Journal article
Palavras-chave: CO2 production; Complex life-cycle; Food quality; Metabolism; Ontogeny.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2011000400005

Implications of the use of glycerin on fed of finishing pigs: review
Provedor de dados: 22
Autores: Corassa, Anderson; Leite, Rafaeli Gonçalves; Silva, Daniela Rocha da; Santos, Tatiane Izabel Silva dos.
Brazilian pig production has been highlighted worldwide, currently the country has occupied the fourth place both in production and export of pork. The the Brazilian governmen has required the addition of 7% biodiesel in fossil diesel, this requirement also increased the amount of glycerin which is a byproduct from production of biodiesel by transesterification. Glycerin has interesting nutritional characteristics for animal feed, and reduce the cost of diets to have energy value similar to corn. For each 90 m3 of biodiesel produced by transesterification of vegetable oils are generated 10 m3 of glycerin. Glycerol is a nutritional component of dietary fat (triglycerides) in the diet can be absorbed as monoglycerides constituent after a partial hydrolysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coproducts; Metabolism; Glycerol; Performance.
Ano: 2017
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/14372

Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures
Provedor de dados: 63
Autores: Nascimento,Valkirea Matos; Silva,Luiziana Ferreira; Gomez,José Gregório Cabrera; Fonseca,Gustavo Graciano.
ABSTRACT Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyhydroxyalkanoates (PHAs); Metabolism; Kinetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500429

Sodium chloride added to transport water and physiological responses of Matrinxã Brycon amazonicus (Teleost: Characidae)
Provedor de dados: 36
Autores: Urbinati,Elisabeth Criscuolo; Carneiro,Paulo César Falanghe.
The addition of salt to the water has been used to mitigate stress and improve survival in fishes. This study investigated the effects of sodium chloride (0.0, 1.0, 3.0 and 6.0 g/l) on levels of plasma cortisol, glucose, tryacilglycerol, total protein, hematocrit, hemoglobin, erythrocyte number, liver glycogen and lipid, and muscle lipid in adult matrinxã(Brycon amazonicum) after a 4-h transport and during a 96-h recovery period. Fish were sampled before and after transport, and 24 and 96 h of the recovery period. Plasma cortisol was higher than initial condition immediately after transportation, except in fish transported in 3.0 and 6.0 g/l of salt. A similar pattern was observed for blood glucose but fish transported in water with 0.0, 1.0 and 3.0 g/l of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salt; Transport; Metabolism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672006000400020

Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides
Provedor de dados: 1
Autores: Cechin, Joanei; Vargas, Leandro; Agostinetto, Dirceu; Lamego, Fabiane Pinto; Mariani, Franciele; Dal Magro, Taísa.
Acetolactate synthase inhibitors are the main group of herbicides used in winter crops in Southern Brazil where their intensive use has selected for herbicide-resistant biotypes of radish. The resistance affects the efficacy of herbicides, and identifying the resistance mechanism involved is important for defining management strategies. The aim of this study was to elucidate the resistance mechanism of radish biotypes by quantifying the enzyme activity, ALS gene sequencing and evaluating the response of biotypes to iodosulfuron and imazethapyr herbicide application after treatment with a cytochrome P450 monooxygenase inhibitor. The susceptible (B1) and resistant (B4 and B13) biotypes were from wheat fields in the Northwest of Rio Grande do Sul State. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.01.02.05-2 Raphanus sativus; Mechanism of resistence; ALS enzyme activity; Gene mutation; Metabolism; Fitossanidade; Herbologia e Resistência de plantas daninhas.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32521

Brazil nut oil in diets for breeder cocks
Provedor de dados: 2
Autores: Rufino, João Paulo Ferreira; Cruz, Frank George Guimarães; Melo, Ramon Duque; Feijó, Julmar da Costa; Melo, Lucas Duque; Costa, Ana Paula Guimarães Cruz; Bezerra, Natalia dos Santos.
 This study aimed to evaluate the inclusion of Brazil nut oil in diets for breeder cocks on performance, semen analyses, biochemical serum profile and testicular morphology. Forty-two Rhode Island Red breeder cocks with 40 weeks of age were used. The experimental design was completely randomized consisting of seven levels of Brazil nut oil (0; 0.30; 0.60; 0.90; 1.20; 1.50 and 1.80%) in the diets. Data collected were evaluated by polynomial regression. Differences (p &lt; 0.05) were detected in feed conversion, semen volume, motility, vigor, pH, swirling and concentration, in which the inclusion of Brazil nut oil in the diets improved the reproductive performance. Differences (p &lt; 0.05) were also detected in the biochemical serum profile (triglycerides,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Additive; Fatty acids; Metabolism; Reproductive performance.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/37472

Farelo da vagem de algaroba associado a níveis de ureia na alimentação de ovinos: balanço de nitrogênio, N-ureico no plasma e parâmetros ruminais - doi: 10.4025/actascianimsci.v34i3.12759
Provedor de dados: 2
Autores: Alves, Evanilton Moura; Instituto Federal Baiano; Pedreira, Márcio dos Santos; Estadual do Sudoeste da Bahia; Pereira, Mara Lúcia Albuquerque; Estadual do Sudoeste da Bahia; Almeida, Paulo José Presidio; Estadual do Sudoeste da Bahia; Gonsalves Neto, João; Estadual do Sudoeste da Bahia; Freire, Leile Daiane Ribeiro; Estadual do Sudoeste da Bahia.
Objetivou-se neste trabalho avaliar os efeitos da inclusão de ureia em dietas contendo farelo da vagem de algaroba sobre o balanço de nitrogênio, N-uréico no plasma e parâmetros ruminais em ovinos. Foram utilizados oito animais, machos castrados, com peso médio de 33,5 kg, distribuídos em dois quadrados latinos 4 x 4. Os tratamentos constituíram da inclusão de níveis de ureia na dieta, sendo: 0; 0,5; 1,0 e 1,5% da MS total. A dieta foi fornecida como dieta total na relação volumoso:concentrado de 40:60 com base na MS total da dieta. Foi realizada a coleta total de urina e fezes e determinada a excreção de nitrogênio. Foi coletado sangue, seguido com a extração do plasma e determinação do N-uréico. Para avaliação do pH e N-amoniacal no líquido ruminal foram...
Tipo: Pesquisa de campo; análise laboratorial
Palavras-chave: Alimento alternativo; Confinamento; Metabolismo; Nitrogênio não protéico; Pequenos ruminantes alternative feed; Confinement; Metabolism; Non-proteic nitrogen; Small ruminants.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/12759

Supplementation of extract of Lafoensia pacari in the diet of semi heavy laying hens
Provedor de dados: 2
Autores: Moreira, Janaina da Silva; Stringhini, José Henrique; Conceição, Edemilson Cardoso da; Arhnold, Emmanuel; Noleto, Raiana Almeida; Carvalho, Fabyola Barros de.
 It was intended to evaluate the supplementation of Lafoensia pacari standardized in tannins extract in the diet of laying hens on the performance, internal and external quality of eggs and metabolism of the feed nutrients. A total of 168 Isa Brown laying hens, aged 24 weeks, with the mean weight of 2.6 kg and the mean posture rate of 87% were used during 4 periods of 28 days each. The treatments consisted of Halquinol performance-enhancing antibiotic, Mannanoligosaccharide (MOS) prebiotic and three levels of pacari extract – 1,000, 2,000 and 3,000 mg kg-1of feed. The experimental design was completely randomized, with six treatments and seven replicates of four hens each. The pacari standardized in tannin extract presented a percentage of albumen and an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.04.00.00-2; 5.04.03.00-1 egg production; Metabolism; Performance; Phytogenic additive.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/32209

Ploidy influences on metabolic substrate deposition of rainbow trout
Provedor de dados: 2
Autores: Gomes, Aline Dal`Olio; Vieira, Vanessa Aparecida Rocha Oliveira; Tabata, Yara Aiko; Takahashi, Neuza Sumico; Moreira, Renata Guimarães; Ribeiro, Cristiele Silva.
Triploid fish are usually sterile. Thus, the energy and nutrients intended for sexual maturation may be available to enhance flesh quality and physical growth. The present study aimed to investigate differences in the metabolic substrates, lipids and proteins, between storage tissues from diploid and triploid female rainbow trout. Monthly, metabolic substrates were quantified in liver, muscle, and ovaries, which were collected during the first reproductive cycle. In general, it was possible to identify a seasonal and similar deposition of metabolites in different tissues of 2n and 3n females, mainly at early stages of gonadal maturation. However, from the stages 5-6, the ovaries showed great differences between ploidies, with higher concentration of lipids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolism; Reproduction; Triploidy; Somatic indexes; Oncorhynchus mykiss.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/44580

Dry and wet diets with and without phytase for growing and finishing pigs
Provedor de dados: 2
Autores: Oliveira, Luiz Alberto Garcia de; Universidade Federal de Goias; Nunes, Romão da Cunha; Universidade Federal de Goias; Rufino, Luciana Moura; Universidade Federal de Goias; Roner, Márcia Nunes Bandeira; Universidade Federal de Sergipe; Stringhini, José Henrique; Universidade Federal de Goias; Di Castro, Izabela Cruvinel; Universidade Federal de Goias.
The present study assessed the effect of adding water and phytase to middling diets on digestibility, metabolism, performance, carcass characteristics, and the bone mineralization of growing and finishing pigs. For the digestibility trial, 16 pigs were distributed in randomized blocks, placed in digestibility cages following a 2 x 2 factorial design (dry and wet diets, with and without phytase). For the performance trial, 48 piglets were distributed into a complete randomized design. During the growing period, wet diets presented higher values for digestibility and energy metabolism. In the finishing stage, the addition of water to the feed caused a greater weight gain and interfered with the carcass length. The balance of the mineral matter and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.04.03.00-1 carcass length; Feed conversion; Digestibility; Metabolism.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/22860

Digestibility of the cottonseed meal with or without addition of protease and phytase enzymes in swine diet - doi: 10.4025/actascianimsci.v34i3.12360
Provedor de dados: 2
Autores: Lorena-Rezende, Izaura Maria Barros de; Universidade Federal Rural de Pernambuco; Dutra Junior, Wilson Moreira; Universidade Federal Rural de Pernambuco; Rezende, Fabio Monteiro; Universidade Federal Rural de Pernambuco; Palhares, Liliane Olimpio; Universidade Federal Rural de Pernambuco; Ludke, Maria do Carmo Mouhaupt Marques; Universidade Federal Rural de Pernambuco; Rabello, Carlos Boa-Viagem; Universidade Federal Rural de Pernambuco.
This study evaluated the digestibility of cottonseed meal with or without addition of enzymes (phytase and protease) for growing pigs. It was used 18 barrows, housed in metabolism cages, distributed in a completely randomized design, standardizing body weight (bw) with average of 25.8 ± 3.6 kg, with three treatments and six repetitions. The treatments consisted of a reference diet based on corn and soybean meal, the second treatment with replacement of 30% of the reference diet by cottonseed meal without enzymes, and the third with 30% of the reference diet replaced by cottonseed meal with added enzymes. Was determined the digestible protein, digestible energy, digestibility of dry matter, energy and protein. It was also registered the balance of nitrogen...
Tipo: Pesquisa de campo
Palavras-chave: Nutrição Animal alternative food; Exogenous enzymes; Metabolism; Non-ruminants.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/12360

Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus
Provedor de dados: 42
Autores: Alves,Gutemberg G.; Marinho-Carvalho,Monica M.; Atella,Georgia C.; Silva-Neto,Mario A.C.; Sola-Penna,Mauro.
6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg) than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, as measured by western-blotting. Muscle extracts are more responsible than fat body to ATP and fructose 6-phosphate, both substrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphate presented a singular pattern for each tissue. Optimal pH (8.0-8.5)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphofructokinase; Metabolism; Insect.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100008

Oral insulin improves metabolic parameters in high fat diet fed rats
Provedor de dados: 42
Autores: LIPINSKI,LEANDRO C.; KMETIUK,LOUISE B.; MATHIAS,PAULO C.F.; MALTA,ANANDA; FAVERO,GIOVANI M.; RIBEIRO,TATIANE A.; TOLEDO,ALCEU; NETTO,MARIO R. MONTEMOR; RODRIGUES,MARCOS R.S.
ABSTRACT Introduction/Aim: The gut has shown to have a pivotal role on the pathophysiology of metabolic disease. Food stimulation of distal intestinal segments promotes enterohormones secretion influencing insulin metabolism. In diabetic rats, oral insulin has potential to change intestinal epithelium behavior. This macromolecule promotes positive effects on laboratorial metabolic parameters and decreases diabetic intestinal hypertrophy. This study aims to test if oral insulin can influence metabolic parameters and intestinal weight in obese non-diabetic rats. Methods: Twelve weeks old Wistar rats were divided in 3 groups: control (CTRL) standard chow group; high fat diet low carbohydrates group (HFD) and HFD plus daily oral 20U insulin gavage (HFD+INS)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral insulin; Intestine; Diabetes; Obesity; Metabolism; Pancreatic beta-cell.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401699

Neutrotoxic effects of fructose administration in rat brain: implications for fructosemia
Provedor de dados: 42
Autores: Macongonde,Ernesto A.; Costa,Naithan L.F.; Ferreira,Bruna K.; Biella,Mairis S.; Frederico,Marisa J.S.; Oliveira,Marcos R. de; Ávila Júnior,Silvio; Silva,Fátima R.M.B.; Ferreira,Gustavo C.; Streck,Emilio L.; Schuck,Patrícia F.
Fructose accumulates in tissue and body fluids of patients affected by hereditary fructose intolerance (HFI), a disorder caused by the deficiency of aldolase B. We investigated the effect of acute fructose administration on the biochemical profile and on the activities of the Krebs cycle enzymes in the cerebral cortex of young rats. Rats received a subcutaneous injection of NaCl (0.9 %; control group) or fructose solution (5 μmol/g; treated group). Twelve or 24 h after the administration, the animals were euthanized and the cerebral cortices were isolated. Peripheral blood (to obtain the serum) and cerebral spinal fluid (CSF) from the animals were also collected. It was observed that albumin levels were decreased and cholesterol levels were increased in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain damage; Fructose; Fructosemia; Hereditary fructose intolerance; Krebs cycle; Metabolism.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301451

Metabolism and secretory function of white adipose tissue: effect of dietary fat
Provedor de dados: 42
Autores: Nascimento,Cláudia M. Oller do; Ribeiro,Eliane B.; Oyama,Lila M.
Approximately 40% of the total energy consumed by western populations is represented by lipids, most of them being ingested as triacylglycerols and phospholipids. The focus of this review is to analyze the effect of the type of dietary fat on white adipose tissue metabolism and secretory function, particularly on haptoglobin, TNF-α, plasminogen activator inhibitor-1 and adiponectin secretion. Previous studies have demonstrated that the duration of the exposure to the high-fat feeding, amount of fatty acid present in the diet and the type of fatty acid may or may not have a significant effect on adipose tissue metabolism. However, the long-term or short-term high fat diets, especially rich in saturated fatty acids, probably by activation of toll-like...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipokines; High fat diets; Metabolism; White adipose tissue.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300010

Effects of insulin and actin on phosphofructokinase activity and cellular distribution in skeletal muscle
Provedor de dados: 42
Autores: Silva,Ana Paula P.; Alves,Gutemberg G.; Araújo,Alexandre H.B.; Sola-Penna,Mauro.
In this work, we report evidences that the association of phosphofructokinase and F-actin can be affected by insulin stimulation in rabbit skeletal muscle homogenates and that this association can be a mechanism of phos-phofructokinase regulation. Through co-sedimentation techniques, we observed that on insulin-stimulated tissues, approximately 70% of phosphofructokinase activity is co-located in an actin-enriched fraction, against 28% in control. This phenomenon is accompanied by a 100% increase in specific phosphofructokinase activity in stimulated homogenates. Purified F-actin causes an increase of 230% in phosphofructokinase activity and alters its kinetic parameters. The presence of F-actin increases the affinity of phosphofructokinase for fructose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphofructokinase; Hormone; Rabbit; Actin; Metabolism; Glycolysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000300008

Phytogenic as feed additive for suckling dairy calves’ has a beneficial effect on animal health and performance
Provedor de dados: 42
Autores: VOLPATO,ANDREIA; CRECENCIO,REGIANE B.; TOMASI,THAINÃ; GALLI,GABRIELA M.; GRISS,LUIZ GUSTAVO; SILVA,ANIÉLEN D. DA; SCHETINGER,MARIA ROSA C.; SCHOGOR,ANA LUIZA B.; BALDISSERA,MATHEUS D.; STEFANI,LENITA M.; SILVA,ALEKSANDRO S. DA.
Abstract: The aim of this study was to evaluate whether the use phytogenic as feed additive of Holstein calves could exert beneficial effects on animal health and performance. Thirty newborn calves were randomly divided into two groups (control and treated), and the treated consisted of 10 g of the phytogenic product diluted in milk once a day for 30 days. Blood and fecal samples were collected, and fecal score was performed daily in order to determine the occurrence of diarrhea. Treated calves showed higher hematocrit on days 45 and 60 of life. Serum levels of urea, total proteins, globulins and glucose were lower in the treated group on day 60 of life, while triglycerides were lower on days 15 and 60 of life. Serum glutathione S-transferase activity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feed; Metabolism; Calves; Oils; Nutrition; Animal health.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700804

Metabolic dynamics of native lambs fed with Tifton 85 hay or Maniçoba hay associated with the spineless cactus
Provedor de dados: 45
Autores: Soares,P.C.; Gouveia,L.N.F.; Silva,T.G.P.; O. Filho,E.F.; Carvalho,F.F.R.; Batista,Â.M.V.; Lima Júnior,D.M.; Cardoso,D.B.
ABSTRACT The objective of this study was to evaluate the metabolic response of native lambs fed Tifton 85 hay (Cynodon dactylon) or Maniçoba hay (Manihot pseudoglaziovii) associated with spineless cactus (Nopalea cochenillifera Salm Dyck). Sixteen Morada Nova lambs were used, with a mean initial weight of 19.36±1.48kg, distributed in a randomized block design, with two treatments and eight replications. Dry matter intake was higher for lambs fed Maniçoba hay compared to Tifton hay, while the body weight at slaughter was not influenced by diets. Regarding the blood metabolites, only the serum concentration of urea was influenced, with higher value observed in the lambs that fed the diet containing Tifton hay. When analyzing blood biomarkers, in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood biomarkers; Clinical biochemistry; Metabolism; Native breeds; Nutrition; Small ruminants.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100215

Glycolysis and gluconeogenesis in the liver of catfish fed with different concentrations of proteins, lipids and carbohydrates
Provedor de dados: 45
Autores: Melo,J.F.B.; Lundstedt,L. M; Inoue,L.A.K.; Metón,I.; Baanante,I.V.; Moraes,G.
ABSTRACT The activities of enzymes from a number of metabolic pathways have been used as a tool to evaluate the best use of nutrients on fish performance. In the present study the catfish Rhamdia quelen was fed with diets containing crude protein-lipid-carbohydrate (%) as follows: treatment (T) T1: 19-19-44; T2: 26-15-39; T3: 33-12-33; and T4: 40-10-24. The fish were held in tanks of re-circulated, filtered water with controlled temperature and aeration in 2000L experimental units. The feeding experiment lasted 30 days. The following enzymes of the carbohydrate metabolism were determined: Glucokinase (GK), Phosphofructokinase 1 (PFK-1), Pyruvate kinase (PK), Fructose-1,6-biphosphatase 1 (FBP-1). The activities of 6 phosphogluconate dehydrogenase (6PGDH)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolism; Enzymes; Nutrition; Adaptation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501251

Mastitis in the transition period: identification of potential blood markers
Provedor de dados: 45
Autores: Santos,K.R.; Souza,F.N.; Blagitz,M.G.; Batista,C.F.; Bertagnon,H.G.; Gomes,R.C.; Molinari,P.C.C.; Diniz,S.A.; Silva,M.X.; Haddad,J.P.A.; Heinemann,M.B.; Libera,A.M.M.P.D.
ABSTRACT This study identified potential blood markers associated with mastitis in dairy cows under different dry therapies during the transition period, using a logistic regression model. Thirty-four Holstein dairy cows were divided into three groups: untreated controls (13 cows, 42 quarters); animals that received an antimicrobial at drying-off (10 cows, 40 quarters); and animals that were administered an internal teat sealant at drying-off (11 cow, 44 quarters). Blood and quarter milk samples were collected 60 days before the expected day of calving, on the day of calving, and three, seven, 15, 21, and 30 days after calving. Milk samples were submitted for bacteriological analysis and somatic cell count. Blood samples were collected for analyses of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intramammary infection; Risk factor; Drying-off; Periparturient period; Metabolism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401120

A model on biological flow of phosphorus in growing pigs
Provedor de dados: 45
Autores: Lopes,J.B.; Moreira,J.A.; Kebreab,E.; Vitti,D.M.S.S.; Abdalla,A.L.; Crompton,L.A.; France,J.
Ten crossbred barrows weighting 20kg were allocated in five groups fed and on diets consisted of different total phosphorus (P) levels: 0.30%, 0.40%, 0.51%, 0.65%, and 0.73%. The animals were intravenously injected 7.4MBq of radioactive P (32P) and after seven days they were slaughtered for further analysis. The evaluated parameters were intake, excretion, specific activities, and flow of P in compartments (1-gut, 2-blood, 3-bone, and 4-soft tissue). Besides fecal losses, the kidneys played an import role in P regulation. The flows of P from tissue and gut to the blood were affected by P intake.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig; Metabolism; Balance; True absorption; Radio-phosphorus; Kinetic.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000300024

Symmorphosis in the proximal pathway for oxygen in the leaf-eared mouse Phyllotis darwini
Provedor de dados: 48
Autores: CANALS,MAURICIO; FIGUEROA,DANIELA; SABAT,PABLO.
In this report, we explore the matching of structures to functional needs by comparing previously reported data of maximal oxygen consumption and the development of the lung in the leaf-eared mouse Phyllotis darwini in warm and cold environments. We discuss whether the state of structural design is commensurate with functional needs from regulated morphogenesis as predicted by the hypothesis of symmorphosis. We found a close match between respiratory structures and functional needs during postnatal development, expressed as safety factors close to unity. However, in the adult stage the safety factors were greater than two, which suggests that adult animals acquired a structure greater than that required considering their maximum capacities. A high safety...
Tipo: Journal article
Palavras-chave: Metabolism; Ontogeny; Phyllotis darwini; Symmorphosis.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100009

Increasing the rate of drying reduces metabolic imbalance, lipid peroxidation and critical water content in radicles of garden pea (Pisum sativum L.)
Provedor de dados: 48
Autores: Ntuli,Tobias M; Pammenter,Norman W; Berjak,Patricia.
Orthodox seeds become desiccation-sensitive as they undergo germination. As a result, germinating seeds serve as a model to study desiccation sensitivity in plant tissues. The effects of the rate of drying on the viability, respiratory metabolism and free radical processes were thus studied during dehydration and wet storage of radicles of Pisum sativum. For both drying regimes desiccation could be described by exponential and inverse modified functions. Viability, as assessed by germination capacity and tetrazolium staining, remained at 100% during rapid (&lt; 24 h) desiccation. However, it declined sharply at c. 0.26 g g¹ dm following slow (c. 5 days) drying. Increasing the rate of dehydration thus lowered the critical water content for survival. Rapid...
Tipo: Journal article
Palavras-chave: Antioxidant; Desiccation; Drying rate; Free radical processes; Lipid peroxidation; Metabolism; Respiration; Pisum sativum; Seed storage.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200002

Metabolic and behavioral effects of ractopamine at continuous low levels in rats under stress
Provedor de dados: 52
Autores: Lopes,Edna; Sousa,Raimundo Vicente de; Zangeronimo,Márcio Gilberto; Pereira,Andressa Naira de Jesus; Coelho,Mariana de Resende; Ferreira,Matheus Soares da Silva; Lima,Renato Ribeiro; Marcondes,Fernanda Klein; Napimoga,Marcelo Henrique; Pereira,Luciano José.
This study aimed at evaluating the effect of ractopamine (RAC) on metabolism, zootechnical performance, body composition, and behavior in Wistar rats submitted to acute and chronic restrain stress. The oral dose of 5 mg/kg of RAC was administered in periods of 0, 7, 14, 21, and 28 days. The elevated plus-maze test (EPMT) was used for behavioral assessment. Blood, carcass and viscera characteristics were evaluated. Insulin-dependent glucose transporters (GLUT-4) were semi-quantified by Western Blot in epididymal adipocytes. RAC periods associated with chronic stress increased the GLUT-4 protein expression in adipose tissue in a time-dependent manner (P=0.01), i.e., the longer the RAC addition period, the higher the GLUT-4 concentration in chronically...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-adrenergic agonist; Ractopamine; Zootechnical performance; Metabolism; Elevated Plus-Maze Tests.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300406

Use of metabolism and swimming activity to evaluate the sublethal toxicity of surfactant (LAS-C12) on Mugil platanus
Provedor de dados: 52
Autores: Barbieri,Edison.
This study aimed to investigate the toxicological effects of the LAS-C12 on Mugil platanus (mullet). Fishes exposed to 1.0 mg.L-1 for 24, 48, 72, 96 and 120 hours presented significant increase in specific routine metabolism. At the concentration of 0.5 mgL-1, the active metabolism presented a decreasing trend from 48 h of exposure on. However, only the consumption averages for 72 h were statistically different from the ones obtained for other periods of exposure. The lowest oxygen consumption in this concentration was observed for 24, 48 and 72 hours of exposure. Significant differences between the control and the concentration of 2.5 mgL-1 were observed for the different periods of exposure. It was not possible to measure the consumption of oxygen for 96...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LAS; Swimming performance; Oxygen consumption; Metabolism; Detergents.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100012

Diets Rich in Polyunsaturated Fatty Acids With Different Omega-6/Omega-3 Ratio Decrease Liver Content of Saturated Fatty Acids Across Generations of Wistar Rats
Provedor de dados: 52
Autores: Halfen,Simone; Jacometo,Carolina Bespalhok; Mattei,Patrícia; Fenstenseifer,Samanta Regine; Pfeifer,Luiz Francisco Machado; Pino,Francisco Augusto Burkert Del; Santos,Marco Aurélio Ziemann; Pereira,Cláudio Martin Pereira de; Schmitt,Eduardo; Corrêa,Marcio Nunes.
Our study evaluated how the consumption of diets with low (LOW group - 0.4/1) or high (CON group - 13.6/1) omega-6/omega-3 ratio across generations (F1 and F2) can modulate liver fatty acid (FA) profile and blood biomarkers. Liver content of α-linolenic acid was higher in animals always fed with LOW diet than animals that changed from CON to LOW diet, which by your time was higher than animals always fed with CON diet. Liver saturated FA concentration decreased in both groups from F1 to F2. In conclusion, both diets were efficient in decreasing the saturated FA liver content across generations, the LOW ratio diet was more effective in reducing blood triglycerides and non-esterified fatty acids, and there was a multigenerational effect of the LOW ratio...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-linolenic acid; Essential fatty acid; Lipid; Metabolism; Nutrition; Rats.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100503

Bone mineral density and vitamin D concentration: the challenges in taking care of children and adolescents infected with HIV
Provedor de dados: 55
Autores: Carmo,Fabiana Bononi; Terreri,Maria Teresa; Succi,Regina Célia de Menezes; Beltrão,Suenia Vasconcelos; Gouvea,Aida de Fátima Tomé Barbosa; Paulino,Erica Regina Cruz; Machado,Daisy Maria.
ABSTRACT  Background: The increase in life expectancy for patients living with human immunodeficiency virus (HIV) infection has resulted in health complications related to a chronic disease.  Objectives: To evaluate the prevalence of bone mineral density (BMD) alterations and vitamin D concentrations in HIV-infected children and adolescents and to verify the variations in those parameters during a 12-month interval.  Methods: A prospective cohort study with a dual period of evaluation was conducted in 57 patients perinatally HIV-infected and one patient with sexual abuse in early infancy. Demographic, anthropometric, pubertal stage, viral load, T CD4+ cell count and antiretroviral therapy were evaluated. Biochemical tests and total body (TB) and lumbar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Antiretroviral; Infant; Diagnosis; Metabolism; Bone.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300270

Chlorophyll treatment combined with photostimulation increases glycolysis and decreases oxidative stress in the liver of type 1 diabetic rats
Provedor de dados: 56
Autores: Wunderlich,A.L.M.; Azevedo,S.C.S.F.; Yamada,L.A.; Bataglini,C.; Previate,C.; Campanholi,K.S.S.; Pereira,P.C.S.; Caetano,W.; Kaplum,V.; Nakamura,C.V.; Nakanishi,A.B.S.; Comar,J.F.; Pedrosa,M.M.D.; Godoi,V.A.F.
Photodynamic therapy (PDT) promotes cell death, and it has been successfully employed as a treatment resource for neuropathic complications of diabetes mellitus (T1DM) and hepatocellular carcinoma. The liver is the major organ involved in the regulation of energy homeostasis, and in pathological conditions such as T1DM, changes in liver metabolic pathways result in hyperglycemia, which is associated with multiple organic dysfunctions. In this context, it has been suggested that chlorophyll-a and its derivatives have anti-diabetic actions, such as reducing hyperglycemia, hyperinsulinemia, and hypertriglyceridemia, but these effects have not yet been proven. Thus, the biological action of PDT with chlorophyll-a on hepatic parameters related to energy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photopigment; Diabetes; Metabolism; Free Radicals; Phototherapy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100601

A case of severe glutathione synthetase deficiency with novel GSS mutations
Provedor de dados: 56
Autores: Xia,H.; Ye,J.; Wang,L.; Zhu,J.; He,Z.
Glutathione synthetase deficiency (GSSD) is a rare inborn error of glutathione metabolism with autosomal recessive inheritance. The severe form of the disease is characterized by acute metabolic acidosis, usually present in the neonatal period with hemolytic anemia and progressive encephalopathy. A case of a male newborn infant who had severe metabolic acidosis with high anion gap, hemolytic anemia, and hyperbilirubinemia is reported. A high level of 5-oxoproline was detected in his urine and a diagnosis of generalized GSSD was made. DNA sequence analysis revealed the infant to be compound heterozygous with two mutations, c.738dupG in exon 8 of GSS gene resulting in p.S247fs and a repetitive sequence in exon 3 of GSS gene. Treatment after diagnosis of GSSD...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Glutathione synthetase; 5-oxoprolinuria; Newborn; Metabolism; Mutation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300501

Glutamine and glutamate as vital metabolites
Provedor de dados: 56
Autores: Newsholme,P.; Lima,M.M.R.; Procopio,J.; Pithon-Curi,T.C.; Doi,S.Q.; Bazotte,R.B.; Curi,R.
Glucose is widely accepted as the primary nutrient for the maintenance and promotion of cell function. This metabolite leads to production of ATP, NADPH and precursors for the synthesis of macromolecules such as nucleic acids and phospholipids. We propose that, in addition to glucose, the 5-carbon amino acids glutamine and glutamate should be considered to be equally important for maintenance and promotion of cell function. The functions of glutamine/glutamate are many, i.e., they are substrates for protein synthesis, anabolic precursors for muscle growth, they regulate acid-base balance in the kidney, they are substrates for ureagenesis in the liver and for hepatic and renal gluconeogenesis, they act as an oxidative fuel for the intestine and cells of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamine; Glutamate; Glucose; Metabolism; Cell function.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200002

Classification of brain tumor extracts by high resolution ¹H MRS using partial least squares discriminant analysis
Provedor de dados: 56
Autores: Faria,A.V.; Macedo Jr.,F.C.; Marsaioli,A.J.; Ferreira,M.M.C.; Cendes,F.
High resolution proton nuclear magnetic resonance spectroscopy (¹H MRS) can be used to detect biochemical changes in vitro caused by distinct pathologies. It can reveal distinct metabolic profiles of brain tumors although the accurate analysis and classification of different spectra remains a challenge. In this study, the pattern recognition method partial least squares discriminant analysis (PLS-DA) was used to classify 11.7 T ¹H MRS spectra of brain tissue extracts from patients with brain tumors into four classes (high-grade neuroglial, low-grade neuroglial, non-neuroglial, and metastasis) and a group of control brain tissue. PLS-DA revealed 9 metabolites as the most important in group differentiation: γ-aminobutyric acid, acetoacetate, alanine,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain; Tumor; Magnetic resonance spectroscopy; Spectroscopy; Metabolism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200009

Involvement of the TRPV1 channel in the modulation of spontaneous locomotor activity, physical performance and physical exercise-induced physiological responses
Provedor de dados: 56
Autores: Hudson,A.S.R.; Kunstetter,A.C.; Damasceno,W.C.; Wanner,S.P.
Physical exercise triggers coordinated physiological responses to meet the augmented metabolic demand of contracting muscles. To provide adequate responses, the brain must receive sensory information about the physiological status of peripheral tissues and organs, such as changes in osmolality, temperature and pH. Most of the receptors involved in these afferent pathways express ion channels, including transient receptor potential (TRP) channels, which are usually activated by more than one type of stimulus and are therefore considered polymodal receptors. Among these TRP channels, the TRPV1 channel (transient receptor potential vanilloid type 1 or capsaicin receptor) has well-documented functions in the modulation of pain sensation and thermoregulatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Afferent; Capsaicin; Hyperthermia; Metabolism; Physical activity; Thermoregulation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600301

Ingestion of a moderate dose of alcohol enhances physical exercise-induced changes in blood lactate concentration
Provedor de dados: 56
Autores: Teixeira-Coelho,F.; Santos,D.F.C.; Santos,G.A.; Sousa,T.F.; Moreira,S.R.; Souza,M.V.C.; Wanner,S.P.
The consumption of alcoholic beverages influences carbohydrate and lipid metabolism, although it is not yet clear whether metabolism during physical exercise at different intensities is also affected. This was the objective of the present study. Eight young and healthy volunteers performed a treadmill test to identify the running speed corresponding to a lactate concentration of 4 mM (S4mM). At least 48 h later, they were subjected to two experimental trials (non-alcohol or alcohol) in which they performed two 1-km running sessions at the following intensities: 1) S4mM; 2) 15% above S4mM. In both trials, blood lactate, triglycerides, and glucose concentrations were measured before and after exercise. The acute alcohol intake increased triglycerides, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endurance; Ethanol; Glucose; Lactate; Metabolism; Treadmill running.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400601

Effect of carbohydrate availability on time to exhaustion in exercise performed at two different intensities
Provedor de dados: 56
Autores: Lima-Silva,A.E.; De-Oliveira,F.R.; Nakamura,F.Y.; Gevaerd,M.S.
This study examined the effects of pre-exercise carbohydrate availability on the time to exhaustion for moderate and heavy exercise. Seven men participated in a randomized order in two diet and exercise regimens each lasting 3 days with a 1-week interval for washout. The tests were performed at 50% of the difference between the first (LT1) and second (LT2) lactate breakpoint for moderate exercise (below LT2) and at 25% of the difference between the maximal load and LT2 for heavy exercise (above LT2) until exhaustion. Forty-eight hours before each experimental session, subjects performed a 90-min cycling exercise followed by 5-min rest periods and a subsequent 1-min cycling bout at 125% VO2max/1-min rest periods until exhaustion to deplete muscle glycogen....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Time to exhaustion; Muscle glycogen; Metabolism; Lactate thresholds.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500002

Metabolism of diglycine and triglycine by non-filtering kidneys
Provedor de dados: 56
We have studied the metabolism of diglycine and triglycine in the isolated non-filtering rat kidney. Kidneys from adult male Wistar Kyoto rats weighing 250-350 g were perfused with Krebs-Henseleit solution containing either 1 mM diglycine or triglycine. The analysis of the peptide residues and their components was performed using an amino acid microanalyzer utilizing ion exchange chromatography. Diglycine was degraded to a final concentration of 0.09 mM after 120 min (91%); this degradation occurred predominantly during the first hour, with a 56% reduction of the initial concentration. The metabolism of triglycine occurred similarly, with a final concentration of 0.18 mM (82%); during the first hour there was a 67% reduction of the initial concentration of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Non-filtering kidney; Dipeptide; Tripeptide; Metabolism; Peptidases.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200005

Oxygen uptake, respiratory exchange ratio, or total distance: a comparison of methods to equalize exercise volume in Wistar rats
Provedor de dados: 56
Autores: Paes,L.S.; Borges,J.P.; Cunha,F.A.; Souza,M.G.C.; Cyrino,F.Z.G.A.; Bottino,D.A.; Bouskela,E.; Farinatti,P.
This study compared strategies to equalize the volume of aerobic exercise performed with different intensities by Wistar rats, based on the distance covered during exercise bouts and energy expenditure (EE, isocaloric sessions) obtained from oxygen uptake (V̇O2) or respiratory exchange ratio (RER). Thirty-three male rats (270.5±12.8 g) underwent maximal exercise tests to determine V̇O2 reserve (V̇O2R), being randomly assigned to three groups: moderate-intensity continuous exercise at speed corresponding to 50% V̇O2R (MIC; n=11); high-intensity continuous exercise at 80% V̇O2R (HIC; n=11); and high-intensity intermittent exercise (HII; n=11) at 60% V̇O2R (3 min) and 80% V̇O2R (4 min). Exercise duration was calculated individually to elicit EE of 5 kcal in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Energy expenditure; Aerobic exercise; Exercise testing; Metabolism; Fitness.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800607

Meal-feeding scheme: twenty years of research in Brazil
Provedor de dados: 56
Autores: Bazotte,R.B.; Batista,M.R.; Curi,R.
Naomi Shinomiya Hell was the first researcher to investigate the physiological adaptations to a meal-feeding scheme (MFS) in Brazil. Over a period of 20 years, from 1979 to 1999, Naomi's group determined the physiological and metabolic adaptations induced by this feeding scheme in rats. The group showed the persistence of such adaptations even when MFS is associated with moderate exercise training and the performance to a session of intense physical effort. The metabolic changes induced by the feeding training were discriminated from those caused by the effective fasting period. Naomi made an important contribution to the understanding of the MFS but a lot still has to be done. One crucial question still remains to be satisfactorily answered: what is the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meal-feeding scheme; Metabolism; Exercise; Fasting; Liver.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900001

Biotransformation of bromhexine by Cunninghamella elegans, C. echinulata and C. blakesleeana
Provedor de dados: 58
Autores: Dube,Aman K.; Kumar,Maushmi S.
Abstract Fungi is a well-known model used to study drug metabolism and its production in in vitro condition. We aim to screen the most efficient strain of Cunninghamella sp. among C. elegans, C. echinulata and C. blakesleeana for bromhexine metabolites production. We characterized the metabolites produced using various analytical tools and compared them with mammalian metabolites in Rat liver microsomes (RLM). The metabolites were collected by two-stage fermentation of bromhexine with different strains of Cunninghamella sp. followed by extraction. Analysis was done by thin layer chromatography, high performance thin layer chromatography, Fourier transform infrared spectroscopy, high performance liquid chromatography and Liquid chromatography–mass...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cunninghamella sp.; Bromhexine; RLM; CYP3A4; Inhibitor; Metabolism.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200259

Lysine catabolism: flow, metabolic role and regulation
Provedor de dados: 61
Autores: Fornazier,Ricardo Francisco; Azevedo,Ricardo Antunes; Ferreira,Renato Rodrigues; Varisi,Vanderlei Aparecido.
Lysine is an essential amino acid, synthesized in plants in the aspartic acid pathway. The lysine catabolism is performed by the action of two consecutive enzymes, lysine 2-oxoglutarate reductase (LOR) and saccharopine dehydrogenase (SDH). The steady state of lysine is controlled by both, synthesis and catabolism rates, with the final soluble lysine concentration in cereal seeds a direct result of these processes. In the last 40 years, the enzymes involved in lysine biosynthesis have been purified and characterized from some plant species such as carrot, maize, barley, rice, and coix. Recent reports have revealed that lysine degradation might be related to various physiological processes, for instance growth, development and response to environmental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acids; Lysine; Metabolism; Mutants.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000100002

Increasing levels of phytase in diets formulated with reduced available phosphorus content supplied to male and female broilers
Provedor de dados: 65
Autores: Naves,Luciana de Paula; Rodrigues,Paulo Borges; Teixeira,Levy do Vale; Bertechini,Antônio Gilberto; Alvarenga,Renata Ribeiro; Bernardino,Verônica Maria Pereira; Santos,Luziane Moreira dos; Natividade,Yuri Pereira Efrem.
The reduction of the available phosphorus (avP) content in the broiler diet must be followed by the supplementation of adequate phytase. One experiment was conducted with broilers from 15 to 28 days of age to determine the level of necessary phytase when the mashed diet is formulated with 0.213% of avP to enable the results of performance, tibia ash content, and calcium (Ca) utilization similar to those determined for birds fed with diet formulated to meet their nutritional requirements. Thus, 120 broilers were distributed in (4+1) x2 factorial arrangement corresponding to four deficient diets in avP (0.213%) supplemented with phytase (0; 750; 1,500 or 2,250FTU kg-1) plus one positive control diet without phytase (0.426% of avP), supplied to male and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aviculture; Bone ash; Enzyme; Metabolism; Performance; Phytate.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801479

Visceral metabolism and efficiency of energy use by ruminants
Provedor de dados: 65
Autores: Kozloski,Gilberto Vilmar; Rocha,João Batista Teixeira da; Ciocca,Maria de Lourdes Santorio.
The visceral system (liver and portal-drained viscera) represents an interface between diet and the animal, and it acts as the main site of regulation of nutrients that are used for maintenance, growth, lactation, reproduction, and physical activities of animals. However the functions carried out by visceral organs have, however, a significant energetic cost and are influenced by a variety of factors, such as the level of feed intake and diet composition, among others. As a result, variable quantities of substances are metabolized by them and, thus, the pattern and the quantity of nutrients available to the peripheral tissues can be quite different from those absorbed at the intestinal lumen. Probably, the major source of variation in the efficiency of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Portal- drained viscera; Liver; Metabolism; Energy; Efficiency.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000500030

Levels of true digestible lisiane and metabolizable energy for growing pigs: effect on nitrogen and energy balance
Provedor de dados: 64
Autores: Chiaradia,Rafael Coutinho Finamor; Zangeronimo,Márcio Gilberto; Fialho,Elias Tadeu; Lima,José Augusto de Freitas; Rodrigues,Paulo Borges; Sousa,Raimundo Vicente de.
An experiment was conducted at the Department of Animal Husbandry of Universidade Federal de Lavras aiming to study the nitrogen and energy balance in diets with reduced crude protein rates (CP) and different levels of energy and lysine for growing pigs. 52 castrated pigs (initial weight = 36.13 ± 2.8 kg) of high genetic potential were used, housed individually in metabolism cages (experimental unit). The treatments consisted of 12 diets with three levels of metabolizable energy (ME) (3060, 3230 and 3400 kcal / kg) and four of true digestible lysine (TDL) (0.7, 0.9, 1.1 and 1, 3%) with 14% CP plus a control diet with 3230 kcal ME / kg, 1.03% TDL and 18% CP. We used a DBC in time 3x4 +1 factorial design with four replications to verify the absorbed nitrogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synthetic amino acids; Nutrition; Metabolism; Ideal protein.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000600028

Nitrogen, calcium and phosphorus balance of broilers fed diets with phytase and crystalline amino acids
Provedor de dados: 64
Autores: Gomide,Elisangela Minati; Rodrigues,Paulo Borges; Zangeronimo,Márcio Gilberto; Bertechini,Antônio Gilberto; Santos,Luziane Moreira dos; Alvarenga,Renata Ribeiro.
The objective of this study was to evaluate the effect of reduced-crude protein (CP) and available phosphorus (aP) diets, added with phytase and amino acids for broilers in the growing (14 to 21 days old) and retirement (35 to 42 days) phases on nitrogen, potassium and phosphorus balance. Ninety Cobb broilers were distributed in a completely randomized design with three treatments and six replications with five and three birds per experimental unit in the growing and retirement phases, respectively. Diets were elaborated to contain reduced CP and aP levels (19 and 17% CP in the growing phase, with 0.34% aP; and 18 and 16% CP in the retirement phase, with 0.30% aP), supplemented with crystalline amino acids and 500 FTU of phytase/kg diet. These were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme; Phytate; Environment; Metabolism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000300022

Diets with reduced levels of nutrients supplemented with phytase and amino acids for broilers
Provedor de dados: 64
Autores: Gomide,Elisangela Minati; Rodrigues,Paulo Borges; Naves,Luciana de Paula; Bernardino,Verônica Maria Pereira; Santos,Luziane Moreira dos; Garcia,Antonio Amandio Pinto.
One performance experiment and another metabolism experiment were conducted simultaneously with broilers from 22 to 35 days of age, evaluating five diets in six replicates. The control diet, without phytase, contained 19% of crude protein (CP); 0.412% of available phosphorus (aP) and 0.824% of calcium (Ca). The other diets were supplemented with phytase and were formulated with 0.262% of aP and 0.524% of Ca, with the CP level ranging from 19 to 16%. In the performance assay, 750 birds were used, in which were evaluated the performance, the tibia ash content and yields of carcass, cuts, and abdominal fat at 35 days of age. The metabolism assay was conducted with 90 birds, evaluating the apparent metabolizable energy corrected by the nitrogen balance; the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tibia ash; Carcass yield; Ideal protein; Metabolism; Performance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000100013