Consulta: subjectFacets:"Metabolomics"
Registros recuperados: 10
Data/hora: 08/06/2024 11:51:03

Exploring the chemodiversity of tropical microalgae for the discovery of natural antifouling compounds
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Réveillon, Damien; Tunin-ley, Alina; Grondin, Isabelle; Othmani, Ahlem; Zubia, Mayalen; Bunet, Robert; Turquet, Jean; Culioli, Gérald; Briand, Jean-françois.
Marine microalgae and cyanobacteria have largely been studied for their biotechnological potential and proved their ability to produce a wide array of bioactive molecules. We investigated the antifouling potential of unexplored benthic tropical microalgae using anti-adhesion and toxicity bioassays against two major micro- and ma crobiofoulers, namely bacteria and barnacles. Fifty strains belonging to six phyla [Cyanobacteria, Miozoa (Dinoflagellata), Bacillariophyta, Cryptophyta, Rhodophyta and Haptophyta] were isolated from southwestern Islands of the Indian Ocean. They were chosen in order to represent as much as possible the huge biodiversity of such a rich tropical ecosystem. The associated chemodiversity was highlighted by both NMR- and LC-MS-based...
Tipo: Text
Palavras-chave: Microalgae; Antifouling; Bioassay; Chemodiversity; Bioprospecting; Metabolomics.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00454/56608/58333.pdf

Preliminary metabolomic approach on cyanobacterial co-cultures: Chemically mediated interactions between Microcystis and Planktothrix
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mondeguer, Florence; Sibat, Manoella; Reubrecht, Sébastien; Amzil, Zouher; Bormans, Myriam; Hess, Philipp; Briand, Enora.
Cyanobacterial proliferation is one of the most harmful hazards, in both freshwater and marine ecosystems. Cyanobacteria are well known for their ability to produce a wide variety of bioactive compounds, some of which have been described as allelochemicals. There is growing evidence that these secondary metabolites play an important role in shaping community composition through biotic interactions; however, for the most part, their biological role and mode of regulation of the production are poorly understood. In temperate eutrophic freshwaters, Microcystis and Planktothrix often co-occur, with Planktothrix being an early colonizer and Microcystis appearing subsequently. By integrating LC-MS/MS molecular networking and an innovative experimental design, we...
Tipo: Text
Palavras-chave: Cyanobacteria; Cyanotoxins; Plankton interactions; Co_culture; LC_HRMS; Molecular Network; Metabolomics.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00405/51666/52215.pdf

Metabolomics based on UHPLC-QToF- and APGC-QToF-MS reveals metabolic pathways reprogramming in response to tidal cycles in the sub-littoral species Mimachlamys varia exposed to aerial emergence
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ory, Pascaline; Bonnet, Antoine; Mondeguer, Florence; Breitwieser, Marine; Dubillot, Emmanuel; Graber, Marianne.
Mimachlamys varia is a sub-littoral bivalve encountered from Norway to the Mediterranean Sea, which lives mostly byssally attached to rocks. During the low tide period, M. varia individuals, located highest on the shore, may experience short time of aerial exposure and face a low availability of oxygen. Here we report a comparative metabolomic profiling of gill samples of M. varia obtained by both LC-QToF and APGC-QToF mass spectrometry, to analyze metabolic changes occurring during emersion in comparison with immersion. Scallops were grown in aquaria with a simulated intertidal environment mimicking short-duration air exposure that they might experience during extreme tides: alternating 2 h emersion and 10 h immersion. Our results show a switch from...
Tipo: Text
Palavras-chave: Anaerobic; APGC-QToF mass spectrometry; Hypoxia; Intertidal; LC-QToF mass spectrometry; Metabolomics; Mimachlamys varia; PLS-DA.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00466/57741/59936.pdf

First insight on interactions between bacteria and the marine diatom Haslea ostrearia : Algal growth and metabolomic fingerprinting
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lepinay, Alexandra; Turpin, Vincent; Mondeguer, Florence; Grandet-marchant, Quentin; Capiaux, Herve; Baron, Regis; Lebeau, Thierry.
A first insight on the interactions between bacteria isolated from monospecific cultures of the marine diatom Haslea ostrearia and this microalgae is described in this work. From 28 bacterial isolates, the maximal growth rate and the maximal cell concentration were on average 58% and 27% higher (max. 67% for isolate B22). Isolates B5, B10 (both Saccharospirillum sp.) and B22 (Rhodobacter sp.) were the most efficient. The geographical origin of H. ostrearia from which bacteria were isolated had no impact on the algal growth performances, and a given bacterial isolate led to the same algal growth irrespective of the geographical origin of H. ostrearia. To explain the results obtained, a comprehensive integrative strategy was conducted that analyzed the...
Tipo: Text
Palavras-chave: Bacterial selection; Plant-growth-promoting bacteria; Phycosphere; Metabolomics.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00429/54036/55340.pdf

Metabolomic Profiles of Dinophysis acuminata and Dinophysis acuta Using Non-Targeted High-Resolution Mass Spectrometry: Effect of Nutritional Status and Prey
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Garcia-portela, Maria; Reguera, Beatriz; Sibat, Manoella; Altenburger, Andreas; Rodriguez, Francisco; Hess, Philipp.
Photosynthetic species of the genus Dinophysis are obligate mixotrophs with temporary plastids (kleptoplastids) that are acquired from the ciliate Mesodinium rubrum, which feeds on cryptophytes of the Teleaulax-Plagioselmis-Geminigera clade. A metabolomic study of the three-species food chain Dinophysis-Mesodinium-Teleaulax was carried out using mass spectrometric analysis of extracts of batch-cultured cells of each level of that food chain. The main goal was to compare the metabolomic expression of Galician strains of Dinophysis acuminata and D. acuta that were subjected to different feeding regimes (well-fed and prey-limited) and feeding on two Mesodinium (Spanish and Danish) strains. Both Dinophysis species were able to grow while feeding on both...
Tipo: Text
Palavras-chave: Dinophysis physiology; Lipophilic toxins; Non-targeted analysis; Metabolomics.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00439/55077/56508.pdf

Serum metabolic profiling of human gastric cancer based on gas chromatography/mass spectrometry
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Song,Hu; Peng,Jun-Sheng; Dong-Sheng,Yao; Yang,Zu-Li; Liu,Huan-Liang; Zeng,Yi-Ke; Shi,Xian-Ping; Lu,Bi-Yan.
Research on molecular mechanisms of carcinogenesis plays an important role in diagnosing and treating gastric cancer. Metabolic profiling may offer the opportunity to understand the molecular mechanism of carcinogenesis and help to non-invasively identify the potential biomarkers for the early diagnosis of human gastric cancer. The aims of this study were to explore the underlying metabolic mechanisms of gastric cancer and to identify biomarkers associated with morbidity. Gas chromatography/mass spectrometry (GC/MS) was used to analyze the serum metabolites of 30 Chinese gastric cancer patients and 30 healthy controls. Diagnostic models for gastric cancer were constructed using orthogonal partial least squares discriminant analysis (OPLS-DA). Acquired...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolomics; Gastric cancer; Gas chromatography/mass spectrometry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100013

Metabolomics as a promising tool for early osteoarthritis diagnosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: de Sousa,E.B.; dos Santos Junior,G.C.; Duarte,M.E.L.; Moura Neto,V.; Aguiar,D.P.
Osteoarthritis (OA) is the main cause of disability worldwide, due to progressive articular cartilage loss and degeneration. According to recent research, OA is more than just a degenerative disease due to some metabolic components associated to its pathogenesis. However, no biomarker has been identified to detect this disease at early stages or to track its development. Metabolomics is an emerging field and has the potential to detect many metabolites in a single spectrum using high resolution nuclear magnetic resonance (NMR) techniques or mass spectrometry (MS). NMR is a reproducible and reliable non-destructive analytical method. On the other hand, MS has a lower detection limit and is more destructive, but it is more sensitive. NMR and MS are useful...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NMR; Metabolomics; Osteoarthritis; Synovial Fluid.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100301

Evaluation of sample extracting methods of FCSM by Lactobacillus acidophilus based on a UPLC-Q-TOF-MS global metabolomics analysis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Wang,Yongqiang; Zhang,Wenju; Nie,Cunxi; Chen,Cheng; Zhang,Xiaoyang; Hu,Jianhe.
Abstract The study of metabolomics requires extracting as many metabolites as possible from a biological sample. This study aimed to determine the optimal method for the extraction of metabolites from solid-state fermented cottonseed meal (FCSM). The UPLC-Q-TOF-MS global metabolomics technology was used to detect the metabolites in FCSM, and the extraction quantity and extraction efficiency of seven different extraction methods, specifically the WA, 50MeOH, 50MeOHB, 50MeCNB, 80MeOHB, 80MeOH and AMF methods were evaluated. The results showed that the number of VIP metabolites extracted by AMF method are 196 and 184 in ESI+ and ESI- mode respectively, it is the largest number of all exacted methods; and the AMF methods also provided a higher extraction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolomics; Extract; Fermentation; FCSM; Lactobacillus acidophilus; UPLC-Q-TOF-MS.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200392

Metabolic analysis of the effect of rheum on a taurocholate-induced acute pancreatitis rat model
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Chen,Zhonghua; Shen,Li; Yang,Changyong; Jiang,Jinghui; Liu,Xing.
ABSTRACT The effects of rheum on serum parameters in a taurocholate-induced acute pancreatitis (AP) rat model were investigated using pathological and biochemical tests, and a proton nuclear magnetic resonance (1H NMR)-based metabonomic strategy. Healthy rats and rats with AP were either treated with rheum (7.5% at a dose of 1.5 g/kg) or left untreated. Serum samples were collected from the AP and rheum-treated groups at 6, 12, and 24 h after treatment. The effect of rheum on pathological changes in the pancreatic was investigated to validate the AP model. We obtained 1H NMR spectra and analyzed the results using the partial least squares discriminant method. The results of the pathological and metabolic analyses revealed an amelioration of multiple...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheum/effects; Acute pancreatitis/analysis; Metabolomics; 1H NMR; Pathological changes; Rats.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200633

Marsdenia tenacissima extract alters crucial metabolites in cancer, determined by 1 H NMR based metabolomics approach
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Roy,Debmalya; Chen,Cheng; Wang,Jun-song; Yuan,Shengtao; Sun,Li.
ABSTRACT Altered metabolites level in the biosystems, is the potential cause of cancer, the primary reason of alteration of metabolism is change in nutrient consumption and waste excretion, as a result genetic mutation leads to cancer initiation and progression. Aberration of specific metabolites such as fumarate, succinate, 2-hydroxyglutarate may alter cell signaling. We collected liver and kidney samples and prepared for 1H NMR analysis, then executed NMR spectroscopy. We used a set of domestic R scripts to perform an unsupervised principal component analysis (PCA) and a supervised orthogonal signal correction partial least-squares discriminant analysis (OSC-PLS-DA). It signifies class discrimination for getting a clear separation, whereas PCA scores...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marsdenia tenacissima extract (MTE); MDA-MB-231; 1H NMR; Metabolomics.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200603