Consulta: subjectFacets:"Metacyclic trypomastigotes"
Registros recuperados: 2
Data/hora: 02/06/2024 10:37:11

Early and late molecular and morphologic changes that occur during the in vitro transformation of Trypanosoma cruzi metacyclic trypomastigotes to amastigotes
Provedor de dados: 48
Autores: CONTRERAS,VÍCTOR T; NAVARRO,MARÍA C; DE LIMA,ANA R; DURAN,FRANCY; ARTEAGA,ROSA; FRANCO,YUNAIMY.
The amastigogenesis primary of T. cruzi occurs naturally when metacyclic trypomastigotes transform into amastigotes within the cells of the mammalian host. The in vitro study of the macromolecular changes that occur over several days during the transformation process should provide significant indications of how the parasite adapts to the mammalian host environment. We show here that metacyclic trypomastigotes pre-incubated at 37&amp;deg; C in a protein-rich medium reach a high degree of transformation to amastigotes when re-incubated in the fresh medium. Giemsa-stained smears show that during the pre-incubation phase, the metacyclic trypomastigotes undergo lengthening at the posterior end and a thinning out of the entire body. SDS-PAGE analysis of...
Tipo: Journal article
Palavras-chave: Amastigogenesis; Differentiation; Macromolecular changes; Metacyclic trypomastigotes; Trypanosoma cruzi.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100008

Signal transduction induced in Trypanosoma cruzi metacyclic trypomastigotes during the invasion of mammalian cells
Provedor de dados: 56
Autores: Yoshida,N.; Favoreto Jr.,S.; Ferreira,A.T.; Manque,P.M.
Penetration of Trypanosoma cruzi into mammalian cells depends on the activation of the parasite's protein tyrosine kinase and on the increase in cytosolic Ca2+ concentration. We used metacyclic trypomastigotes, the T. cruzi developmental forms that initiate infection in mammalian hosts, to investigate the association of these two events and to identify the various components of the parasite signal transduction pathway involved in host cell invasion. We have found that i) both the protein tyrosine kinase activation, as measured by phosphorylation of a 175-kDa protein (p175), and Ca2+ mobilization were induced in the metacyclic forms by the HeLa cell extract but not by the extract of T. cruzi-resistant K562 cells; ii) treatment of parasites with the tyrosine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Signal transduction; Metacyclic trypomastigotes; Protein kinase; Calcium response; Cell invasion.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300003