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An Assessment of Environmental Metabarcoding Protocols Aiming at Favoring Contemporary Biodiversity in Inventories of Deep-Sea Communities
Provedor de dados: 5
Autores: Brandt, Miriam; Trouche, Blandine; Henry, Nicolas; Liautard-haag, Cathy; Maignien, Lois; De Vargas, Colomban; Wincker, Patrick; Poulain, Julie; Zeppilli, Daniela; Arnaud-haond, Sophie.
The abyssal seafloor covers more than 50% of planet Earth and is a large reservoir of still mostly undescribed biodiversity. It is increasingly targeted by resource-extraction industries and yet is drastically understudied. In such remote and hard-to-access ecosystems, environmental DNA (eDNA) metabarcoding is a useful and efficient tool for studying biodiversity and implementing environmental impact assessments. Yet, eDNA analysis outcomes may be biased toward describing past rather than present communities as sediments contain both contemporary and ancient DNA. Using commercially available kits, we investigated the impacts of five molecular processing methods on eDNA metabarcoding biodiversity inventories targeting prokaryotes (16S), unicellular...
Tipo: Text
Palavras-chave: Environmental metabarcoding; RNA versus DNA; Extracellular DNA; Deep-sea biodiversity; Benthic ecology; Biomonitoring; Method testing.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00630/74165/73774.pdf