Consulta: subjectFacets:"Microalgues"
Registros recuperados: 13
Data/hora: 05/06/2024 14:12:03

La métabolomique et les biotoxines marines
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mondeguer, Florence.
Metabolomics is a new approach to assess the contamination of organisms, environmental compartments or foodstuffs, and the impact marine pollution, (natural or anthropogenic), can have on them. Through the analysis of the variation of the metabolite profiles present in the cell, tissues, organs or even the whole organism it is possible to define a physiological state, a stage of development, or the level and nature of a contamination. Generally, analysis of metabolites correlates to experimental design built around a clear biological or environmental question, needs to be supported by sensitive and reproducible tools, and requires access to libraries "house" or international. Often, the non-targeted approach allows for evaluation of many hypotheses. Thus,...
Tipo: Text
Palavras-chave: Biotoxines; Biomarqueurs; Métabolomique; Microalgues; Moules; Spectrométrie de masse haute résolution (HRMS); Workflow.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00358/46892/46777.pdf

The food quality of Tetraselmis suecica slurry for Crassostrea gigas spat
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Robert, Rene; Parisi, Guilina; Pastorelli, Roberta; Poli, Bianca Maria; Tredici, Mario.
In an attempt to provide cost-effective alternatives to live microalgae and to simplify hatchery-nursery procedures, concentrated microalgae appear to be very promising. Due to its high preservation ability, Prasinophytes appear to be good candidates for such alternative diets. Nevertheless, in a previous study the utilization of Tetraselmis suecica was only conceivable for old Crassostrea gigas larvae. In the present work its nutritional quality has been accordingly searched on ]apanese oyster's juveniles. Whatever the assemblages and condition, fresh or preserved microalgae, T. suecica did not allow an adequate postlarval development in C. gigas.
Tipo: Text
Palavras-chave: Nutritional quality; Crassostrea gigas; Tetraselmis suecica; Microalgae; Valeur alimentaire; Crassostrea gigas; Tetraselmis suecica; Microalgues.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-6202.PDF

Maitrise de la culture de masse de la diatomée Skeletonema costatum en système ouvert et continu sur un milieu en "eau de mer"
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Brossard, Nicolas; Hussenot, Jerome.
Une culture extérieure en masse de la diatomée Skeletonema costatum, en système ouvert et continu, a été réalisée en automne 1996, au marais expérimental du CREMA I'Houmeau. Le but était d'apporter une nourriture complémentaire aux huîtres en période d'affinage.
Tipo: Text
Palavras-chave: Phytoplancton; Microalgues; Diatomées; Skeletonema costatum; Culture de masse; Culture continue; Affinage d'huîtres; Phytoplankton; Diatoms; Skeletonema costatum; Mass culture; Continuous culture; Oyster fattening.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00020/13117/10126.pdf

Etude de nouvelles cibles moleculaires de cancer bronchopulmonaire non a petites cellules pharmacomodulées par des substances originales naturelles et synthetiques
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Moreau, Dimitri.
Still rare disease at the beginning of the 20th century, the lung cancer is at the origin of more than 6 % of the deaths in France currently. It occupies there the first rank of the deaths by cancer whereas France is regarded as a country at the average risk among the Western countries. The discovery of new anti-cancer treatments increasingly more effective and specific, is thus today of primary importance.  The validation of the capacity of a molecule to being able to inhibit the cancerous proliferation, passes by several stages of tests starting with the description of an activity at the cellular level. One of the strategies used for the discovery of new compounds antiproliferativs, passes by an investigation of the bioactiv potential of a great number...
Tipo: Text
Palavras-chave: Microalgae; Natural products; Apoptosis; Antiproliferativ; Gene expression; Regulatory RNA; Molecular targets; Bronchopulmonary cancer; Microalgues; Molécules naturelles; Apoptose; Antiprolifératif; Expression génique; ARN régulateur; Cibles moléculaires; Cancer bronchopulmonaire.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/these-2196.pdf

Test écotoxicologique sur microalgues et lecteur de plaques : mesures d'activités enzymatiques sur cellules in vivo. Application à l'évaluation de la qualité du milieu
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gilbert, Franck; Galgani, Francois.
A new method for the assessment of several contaminant effects on marine microalgae (Tetraselmis suesica, Skeletonema costatum) has been developped. The method is based on the measure of cells esterase activity using a fluorimetric stain, fluorescein diacetate (FDA). The experiments are performed in microplates using a fluorimetric microplate reader. The different steps of the experiments and the data processing are controlled by an original specific software. Application of the system for rapid ecotoxicological tests (determination of LC 50) and for assessment of environment quality by observation of water samples are proposed.
Tipo: Text
Palavras-chave: Environnement; Écotoxicologie; CL 50; Estérase; Microalgues; FDA; Microplaques; Environment; Ecotoxicology; Le 50; Esterase; Microalgae; FDA; Microplates.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00156/26727/24808.pdf

Caractérisation morphologique et physiologique des microalgues utilisées pour la nutrition larvaire de l'huître creuse, Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Gourrierec, Gaétane.
En aquaculture, sur une cinquantaine d'algues testées, seulement une douzaine présente un réel intérêt en écloserie-nurserie de bivalves (Chrétiennot-Dinet et al, 1986). Le premier but de notre travail est de caractériser 11 espèces de microalgues au cytomètre en flux afin de fournir un outil descriptif, facilement réutilisable, de ces espèces et d' évaluer l' influence de l'état physiologique et de la méthode de fixation sur les signatures cytométriques. Les paramètres mesurés au cytomètre en flux sont la FSC, la FL3 et la SSC liées respectivement à la taille, la fluore scence et la structure des cellules phytoplanctoniques. Les résultats montrent que les signatures sont extrêmement variables entre espèces et à l' intérieur d' une espèce. Seules, deux...
Tipo: Text
Palavras-chave: Microalgues; Cytométrie en flux; Caractérisation; Viabilité cellulaire; Larves d'huîtres.
Ano: 2002
URL: https://archimer.ifremer.fr/doc/00448/55962/57446.pdf

Influence de la matière organique dissoute naturelle et d’herbicides, seuls et combinés, sur des microalgues marines et d’eau douce
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Coquillé, Nathalie.
Microalgae are at the basis of aquatic food webs and may be the indirect target of herbicides from agricultural and urban uses. They also interact with other compounds from their environment such as natural dissolved organic matter (DOM), which can itself interact with herbicides. This thesis aimed to study, in laboratory controlled conditions, the influence of natural DOM on herbicide toxicity to microalgae by using monospecific, non-axenic cultures. The experiments included: natural freshwater and marine DOM, three herbicides (irgarol, diuron and S-metolachlor) singly and in mixtures, two freshwater microalgae (Gomphonema gracile and Sphaerellopsis sp.) and two marine microalgae (Chaetoceros calcitrans and Tetraselmis suecica). Effects were evaluated on...
Tipo: Text
Palavras-chave: Microalgues; Matière organique dissoute naturelle; MOD; Herbicides; Transformations chimiques; Toxicité.; Microalgae; Natural dissolved organic matter; DOM; Herbicides; Chemical transformations; Toxicity.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00461/57245/59250.pdf

Interactions microalgues-bactéries en système expérimental bispécifique : effets sur la croissance de Dunaliella sp.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Chevanton, Myriam.
The use of algae-bacteria mixed culture has been suggested to improve performance of industrial microalgae culture. The present study aims to investigate impact of selected bacteria on Dunaliella sp. growth. 97 bacteria strains were isolated from a variety of marine microalgae cultures. The diversity of strains revealed members of α-and γ-proteobacteria, Cytophaga-Flavobacterium-Bacteroides and grampositive. A two-phase approach was then implemented to select bacteria that improve Dunaliella sp. growth, using an original high-throughput experimental device. Although a majority of bacteria induced performance reduction for Dunaliella sp., some resulted in significant improvements. In nitrogenlimited batch culture, the Alteromonas sp. bacteria demonstrated a...
Tipo: Text
Palavras-chave: Interactions; Microalgues; Bactéries; Dunaliella; Axénie; Interactions; Microalgae; Bacteria; Dunaliella; Axenic.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00187/29839/28291.pdf

Microalgues marines : les enjeux de la recherche
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Muller-feuga, Arnaud.
Industrial potentialities related to biotechnology of microalgae are examined, highlighting a wide spectrum of applications which concern principally nutrition, health, environment and energy. The french situation of research on microalgae, especially at Ifremer, is assessed, showing that the main axis are fine chemistry, aquaculture, environment, bio-process engineering. It covers the majority of the applications, but leaves in shade certain important aspects such as human food and CO2 fixation. Concerning the general approach, some significant improvements can be expected from a more accurate control of bacteriological and genetic quality of cultures. About the industrial aspects, a national initiative seems in progress. The variety of potential...
Tipo: Text
Palavras-chave: Process engineering; Energy; Environment; Health; Food; Aquaculture; Biotechnology; Microalgae; Génie des procédés; Energie; Environnement; Santé; Alimentation; Aquaculture; Biotechnologie; Microalgues.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/rapport-1092.pdf

Suivi des efflorescences phytoplanctoniques en Charente-Maritime en 1988
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Burgeot, Thierry; Fillon, Alain; Ratiskol, Gilles; Vayne, Jean-jacques.
La synthèse des informations collectées en 1988 en Charente Maritime, en matière de développement microalgaux a pour but de mettre en évidence, à partir des données recueillies au cours d'une année, des tendances et niveaux de contaminations phytoplanctoniques, ainsi qu'une certaine identité du secteur surveillé. L'année 1988 est marquée par une efflorescence monospécifique de Gymnodinium sp. en période estivale et par l'apparition du Dinophysis à des concentrations supérieures au seuil toxique sans qu'aucne toxicité ne soit décelée. Le réseau de suivi confirme, à travers la bonne représentativité des stations de prélèvement, la spécificité des pertuis charentais
Tipo: Text
Palavras-chave: Microalgues; Pertuis charentais; Efflorescences monospécifiques.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00443/55496/57039.pdf

Les besoins écophysiologiques des larves d'huitres creuse Crassostrea gigas en conditions contrôlees : effet de la température, de la nourriture et modélisation de la croissance
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rico Villa, Benjamin.
Nowadays, spat production of Pacific oyster Crassostrea gigas relies on rearing techniques under controlled conditions in hatchery. In this context, an extensive research in aquaculture is necessary because larval development and metamorphosis are critical for hatchery management. Firstly, a diet consisted in a Haptophyceae: Isochrysis affinis galbana (clone T-ISO) and a Bacillariophyceae: Chaetoceros calcitrans forma pumilum demonstrated its efficiency to supply the nutritional needs throughout the whole larval rearing. Then, a flow-through rearing system was developed and coupled with a continuous hydrobiological data recorder to provide basic information for ecophysiology research through different trophic levels and/or temperature throughout larval...
Tipo: Text
Palavras-chave: Crassostrea gigas; Larve; Huître; Croissance; Ecophysiologie; Microalgues; Température; Système d’élevage; Modélisation; Crassostrea gigas; Larvae; Oyster; Growth; Ecophysiology; Microalgae; Temperature; Rearing System; Modelling.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00012/12308/9102.pdf

Allocation du carbone et métabolisme azoté chez l'haptophyte Tisochrysis lutea
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Garnier, Matthieu.
The aim of this thesis is to improve knowledge on mechanisms involved in the response to nitrogen limitation and in lipid accumulation in the microalgae haptophyte Tisochrysis lutea. The wild type strain and a lipid accumulating mutant strain were grown under different nitrogen limitation and starvation and analyzed by functional genomics. Four genes of high-affinity nitrate/nitrite transporter (Nrt2) were identified and characterized to reveal the mechanisms involved in mineral absorption in this species. Transcriptomes of both strains were sequenced and proteins affected by nitrogen starvation and differentially expressed between the two strains were identified. We so identified the functions regulated by nitrogen deficiency and potentially involved in...
Tipo: Text
Palavras-chave: Microalgues; Métabolisme; Azote; Carbone; Lipides; Genomique fonctionelle; Protéomique; Transcriptomique; Microalgae; Metabolism; Nitrogen; Carbon; Lipids; Functional genomics; Proteomics; Transcriptomics.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00353/46378/46000.pdf

Valeur alimentaire de Tetraselmis striata et T. chui pour les larves de Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ponis, Emanuele; Parisi, Guilina; Robert, Rene.
In an attempt to simplify mollusc hatchery-nursery procedures and to provide cost-effective alternative diets, the use of concentrated and preserved microalgae is an alternative to the use of fresh microalgae. Because of their ability for preservation, Prasinophytes appears to be good candidates for remote algal production. In the present work, the food value of fresh and preserved Tetraselmis chui and T. striata for umboned Crassostrea gigas larvae was studied. During the first experiment, fresh T. striata led to the best larval development, but these performances were not confirmed subsequently. When used as monospecific diets the food value of both Prasinophytes is unsufficient to support a good C. gigas larval development. However in bispecific diets a...
Tipo: Text
Palavras-chave: Food value; Microalgae; Crassostrea gigas; Tetraselmis chui; Tetraselmis striata; Valeur alimentaire; Microalgues; Crassostrea gigas; Tetraselmis chui; Tetraselmis striata.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-6203.PDF